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Conserved domains on  [gi|86562726|ref|NP_001033350|]
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putative tRNA (uracil-O(2)-)-methyltransferase [Caenorhabditis elegans]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 4222)

class I SAM-dependent methyltransferase that catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to tRNA (uracil-O(2)-)-methyltransferase that catalyzes the formation of O(2)-methyluracil at position 44 (Um44) in tRNA(Ser)

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:0008168|GO:1904047
PubMed:  12504684|12826405
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdoMet_MTase super family cl06678
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 186-294 6.25e-22

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


The actual alignment was detected with superfamily member pfam07757:

Pssm-ID: 462255  Cd Length: 115  Bit Score: 91.24  E-value: 6.25e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726   186 LLDKESYIKTYRHIREDYGRGMIKGWT---ENSNPQKSIFEDCGIASYINEL-VNSDLLPKPNKFVDIGCGNGLLVHLLN 261
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHAKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELwKQTQSENKEFAFADLGCGNGLLVHLLL 80

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 86562726   262 KIGMSGYGIDVRHRNIWKTTLKDVD--LREMPVDP 294
Cdd:pfam07757  81 AEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
 
Name Accession Description Interval E-value
AdoMet_MTase pfam07757
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 186-294 6.25e-22

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


Pssm-ID: 462255  Cd Length: 115  Bit Score: 91.24  E-value: 6.25e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726   186 LLDKESYIKTYRHIREDYGRGMIKGWT---ENSNPQKSIFEDCGIASYINEL-VNSDLLPKPNKFVDIGCGNGLLVHLLN 261
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHAKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELwKQTQSENKEFAFADLGCGNGLLVHLLL 80

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 86562726   262 KIGMSGYGIDVRHRNIWKTTLKDVD--LREMPVDP 294
Cdd:pfam07757  81 AEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 229-306 1.96e-04

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 42.60  E-value: 1.96e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726 229 SYINELVNSDLLPKPNKFVDIGCGNG-LLVHLLNKIGMSGYGIDV--------RHRNIwKTTLKDVDLREMPVDpQIVVQ 299
Cdd:COG0500  13 GLAALLALLERLPKGGRVLDLGCGTGrNLLALAARFGGRVIGIDLspeaialaRARAA-KAGLGNVEFLVADLA-ELDPL 90


                ....*..
gi 86562726 300 NEPHFDL 306
Cdd:COG0500  91 PAESFDL 97
 
Name Accession Description Interval E-value
AdoMet_MTase pfam07757
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 186-294 6.25e-22

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


Pssm-ID: 462255  Cd Length: 115  Bit Score: 91.24  E-value: 6.25e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726   186 LLDKESYIKTYRHIREDYGRGMIKGWT---ENSNPQKSIFEDCGIASYINEL-VNSDLLPKPNKFVDIGCGNGLLVHLLN 261
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHAKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELwKQTQSENKEFAFADLGCGNGLLVHLLL 80

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 86562726   262 KIGMSGYGIDVRHRNIWKTTLKDVD--LREMPVDP 294
Cdd:pfam07757  81 AEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 229-306 1.96e-04

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 42.60  E-value: 1.96e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726 229 SYINELVNSDLLPKPNKFVDIGCGNG-LLVHLLNKIGMSGYGIDV--------RHRNIwKTTLKDVDLREMPVDpQIVVQ 299
Cdd:COG0500  13 GLAALLALLERLPKGGRVLDLGCGTGrNLLALAARFGGRVIGIDLspeaialaRARAA-KAGLGNVEFLVADLA-ELDPL 90


                ....*..
gi 86562726 300 NEPHFDL 306
Cdd:COG0500  91 PAESFDL 97
Methyltransf_23 pfam13489
Methyltransferase domain; This family appears to be a methyltransferase domain.
 227-306 1.31e-03

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 404385 [Multi-domain]  Cd Length: 162  Bit Score: 39.72  E-value: 1.31e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726   227 IASYINELvnSDLLPKPNKFVDIGCGNGLLVHLLNKIGMSGYGIDVRHRNIWKTTLKDVDLremPVDPQIVVQNEPHFDL 306
Cdd:pfam13489   9 LADLLLRL--LPKLPSPGRVLDFGCGTGIFLRLLRAQGFSVTGVDPSPIAIERALLNVRFD---QFDEQEAAVPAGKFDV 83
UbiG COG2227
2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and ...
 215-306 3.99e-03

2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase [Coenzyme transport and metabolism]; 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase is part of the Pathway/BioSystem: Ubiquinone biosynthesis


Pssm-ID: 441829 [Multi-domain]  Cd Length: 126  Bit Score: 37.69  E-value: 3.99e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726 215 SNPQKSIFEDCGIASYINelvnsDLLPKPNKFVDIGCGNGLLVHLLNKIGMSGYGIDV---------RHRNIWKTTLKDV 285
Cdd:COG2227   2 SDPDARDFWDRRLAALLA-----RLLPAGGRVLDVGCGTGRLALALARRGADVTGVDIspealeiarERAAELNVDFVQG 76

                        90       100
                ....*....|....*....|.
gi 86562726 286 DLREMPVDPQivvqnepHFDL 306
Cdd:COG2227  77 DLEDLPLEDG-------SFDL 90
Methyltransf_32 pfam13679
Methyltransferase domain; This family appears to be a methyltransferase domain.
 227-340 9.94e-03

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 379330 [Multi-domain]  Cd Length: 138  Bit Score: 36.78  E-value: 9.94e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 86562726   227 IASYINELVNSdllPKPNKFVDIGCGNGLLVHLLN--KIGMSGYGIDVRHRNIWKTTLKDVDLR---EMpVDPQIVVQNE 301
Cdd:pfam13679  13 IAPLLKELLDE---NGPITIVDHGAGKGYLGFILYylKYGVRVYGIDTRAELVEKANALAQKLGfnkRM-SFLEGTIAGS 88

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 86562726   302 PHFDLD--VDLLIGNHS-DELTPWIPVMAAKLNCNF-FLIPCC 340
Cdd:pfam13679  89 TPVELPdrVDVVTALHAcDTATDDALRFALAKQARAiVLVPCC 131
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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