NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|442632302|ref|NP_001261838|]
View 

stonewall, isoform B [Drosophila melanogaster]

Protein Classification

SANT/Myb-like DNA-binding domain-containing protein( domain architecture ID 11045548)

SANT (SWI3, ADA2, N-CoR and TFIIIB)/Myb-like DNA-binding domain-containing protein binds DNA and may function as a transcription factor

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
BESS pfam02944
BESS motif; The BESS motif is named after the proteins in which it is found (BEAF, Suvar(3)7 ...
 566-599 1.20e-09

BESS motif; The BESS motif is named after the proteins in which it is found (BEAF, Suvar(3)7 and Stonewall). The motif is 40 amino acid residues long and is composed of two predicted alpha helices. Based on the protein in which it is found and the presence of conserved positively charged residues it is predicted to be a DNA binding domain. This domain appears to be specific to drosophila.


:

Pssm-ID: 460758  Cd Length: 35  Bit Score: 54.32  E-value: 1.20e-09
                          10        20        30
                  ....*....|....*....|....*....|....
gi 442632302  566 SDNYFLEMIKPQMQEMNPRQKMHFKKKVFQALME 599
Cdd:pfam02944   2 SDDLFLLSLLPTMKRLPPKQKLKFKIKILQLLQE 35
GT1 super family cl23759
GT1, myb-like, SANT family; GT-1, a myb-like protein, is one of the GT trihelix transcription ...
 13-60 7.58e-09

GT1, myb-like, SANT family; GT-1, a myb-like protein, is one of the GT trihelix transcription factors. GT-1 binds the GT cis-element of rbcS-3A, a light-induced gene, as a dimer. Arabidopsis GT-1 is a trans-activator and acts in the stabilization of components of the transcription pre-initiation complex comprised of TFIIA-TBP-TATA. The isolated GT-1 DNA-binding domain is sufficient to bind DNA. This region closely resembles the myb domain, but with longer helices. It has been proposed that GT-1 may respond to light signals via calcium-dependent phosphorylation to create a light-modulated molecular switch. These proteins are members of the SANT/myb group. SANT is named after 'SWI3, ADA2, N-CoR and TFIIIB', several factors that share this domain. The SANT domain resembles the 3 alpha-helix bundle of the DNA-binding Myb domains and is found in a diverse set of proteins.


The actual alignment was detected with superfamily member smart00595:

Pssm-ID: 474047  Cd Length: 89  Bit Score: 53.52  E-value: 7.58e-09
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 442632302    13 EKCKRRWRQLRNDYTRWCNADANRRRNGQRRLAYPLADELRFLDRHLN 60
Cdd:smart00595  42 EECKKRWKNLRDRYRRELKRLQNGKSGGGKKSKWEYFDRLSFLRPVIR 89
COG5177 super family cl34927
Uncharacterized conserved protein [Function unknown];
93-229 6.05e-03

Uncharacterized conserved protein [Function unknown];


The actual alignment was detected with superfamily member COG5177:

Pssm-ID: 227504 [Multi-domain]  Cd Length: 769  Bit Score: 40.45  E-value: 6.05e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442632302  93 QASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEK-------------LNDLEESDEPEKV 159
Cdd:COG5177  302 QYEQTIREIFADRATKLELDLQTVFESNMNRDTLDEYAPEGEDLRSDYDEDFEYdglttvriddhgfLPGREQTSKKAAV 381

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 442632302 160 PELDSFLQS------DNEDDECMDEEHLEDLEGFDFDLEQSNQEKELTPEKSLEKNNTDSTVSqPEFFVKQTRDPR 229
Cdd:COG5177  382 PKGTSFYQAkwaedeEEEDGQCNDEESTMSAIDDDDPKENDNEEVAGDEESAIDDNEGFEELS-PEEEERQLREFR 456
 
Name Accession Description Interval E-value
BESS pfam02944
BESS motif; The BESS motif is named after the proteins in which it is found (BEAF, Suvar(3)7 ...
 566-599 1.20e-09

BESS motif; The BESS motif is named after the proteins in which it is found (BEAF, Suvar(3)7 and Stonewall). The motif is 40 amino acid residues long and is composed of two predicted alpha helices. Based on the protein in which it is found and the presence of conserved positively charged residues it is predicted to be a DNA binding domain. This domain appears to be specific to drosophila.


Pssm-ID: 460758  Cd Length: 35  Bit Score: 54.32  E-value: 1.20e-09
                          10        20        30
                  ....*....|....*....|....*....|....
gi 442632302  566 SDNYFLEMIKPQMQEMNPRQKMHFKKKVFQALME 599
Cdd:pfam02944   2 SDDLFLLSLLPTMKRLPPKQKLKFKIKILQLLQE 35
MADF smart00595
subfamily of SANT domain;
13-60 7.58e-09

subfamily of SANT domain;


Pssm-ID: 214738  Cd Length: 89  Bit Score: 53.52  E-value: 7.58e-09
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 442632302    13 EKCKRRWRQLRNDYTRWCNADANRRRNGQRRLAYPLADELRFLDRHLN 60
Cdd:smart00595  42 EECKKRWKNLRDRYRRELKRLQNGKSGGGKKSKWEYFDRLSFLRPVIR 89
MADF_DNA_bdg pfam10545
Alcohol dehydrogenase transcription factor Myb/SANT-like; The myb/SANT-like domain in Adf-1 ...
 1-55 6.25e-07

Alcohol dehydrogenase transcription factor Myb/SANT-like; The myb/SANT-like domain in Adf-1 (MADF) is an approximately 80-amino-acid module that directs sequence specific DNA binding to a site consisting of multiple tri-nucleotide repeats. The MADF domain is found in one or more copies in eukaryotic and viral proteins and is often associated with the BESS domain. It is likely that the MADF domain is more closely related to the myb/SANT domain than it is to other HTH domains.


Pssm-ID: 463144  Cd Length: 84  Bit Score: 48.05  E-value: 6.25e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 442632302    1 MMGDFLFENSCLEKCKRRWRQLRNDYTRWCNAdANRRRNGQRRLAYPLADELRFL 55
Cdd:pfam10545  31 EIAEELGSDVPVEDCKKRWKNLRDQYRRELRR-KRTNSGELYKSRWYYYEELSFL 84
COG5177 COG5177
Uncharacterized conserved protein [Function unknown];
93-229 6.05e-03

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227504 [Multi-domain]  Cd Length: 769  Bit Score: 40.45  E-value: 6.05e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442632302  93 QASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEK-------------LNDLEESDEPEKV 159
Cdd:COG5177  302 QYEQTIREIFADRATKLELDLQTVFESNMNRDTLDEYAPEGEDLRSDYDEDFEYdglttvriddhgfLPGREQTSKKAAV 381

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 442632302 160 PELDSFLQS------DNEDDECMDEEHLEDLEGFDFDLEQSNQEKELTPEKSLEKNNTDSTVSqPEFFVKQTRDPR 229
Cdd:COG5177  382 PKGTSFYQAkwaedeEEEDGQCNDEESTMSAIDDDDPKENDNEEVAGDEESAIDDNEGFEELS-PEEEERQLREFR 456
PRK05901 PRK05901
RNA polymerase sigma factor; Provisional
 74-230 8.52e-03

RNA polymerase sigma factor; Provisional


Pssm-ID: 235640 [Multi-domain]  Cd Length: 509  Bit Score: 39.98  E-value: 8.52e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442632302  74 SSDKDRDNNRDSEGVDHHAQASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEKLNDLEES 153
Cdd:PRK05901  45 KKKTPEQIDQVLIFLSGMVKDTDDATESDIPKKKTKTAAKAAAAKAPAKKKLKDELDSSKKAEKKNALDKDDDLNYVKDI 124

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 442632302 154 DEPEKVPELDSFLQSDNEDDECMDEEHLEDLEGFDFDLEQSNQEKE-LTPEKSLEKNNTDSTVSQPEFFVKQTRDPRL 230
Cdd:PRK05901 125 DVLNQADDDDDDDDDDDLDDDDIDDDDDDEDDDEDDDDDDVDDEDEeKKEAKELEKLSDDDDFVWDEDDSEALRQARK 202
 
Name Accession Description Interval E-value
BESS pfam02944
BESS motif; The BESS motif is named after the proteins in which it is found (BEAF, Suvar(3)7 ...
 566-599 1.20e-09

BESS motif; The BESS motif is named after the proteins in which it is found (BEAF, Suvar(3)7 and Stonewall). The motif is 40 amino acid residues long and is composed of two predicted alpha helices. Based on the protein in which it is found and the presence of conserved positively charged residues it is predicted to be a DNA binding domain. This domain appears to be specific to drosophila.


Pssm-ID: 460758  Cd Length: 35  Bit Score: 54.32  E-value: 1.20e-09
                          10        20        30
                  ....*....|....*....|....*....|....
gi 442632302  566 SDNYFLEMIKPQMQEMNPRQKMHFKKKVFQALME 599
Cdd:pfam02944   2 SDDLFLLSLLPTMKRLPPKQKLKFKIKILQLLQE 35
MADF smart00595
subfamily of SANT domain;
13-60 7.58e-09

subfamily of SANT domain;


Pssm-ID: 214738  Cd Length: 89  Bit Score: 53.52  E-value: 7.58e-09
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 442632302    13 EKCKRRWRQLRNDYTRWCNADANRRRNGQRRLAYPLADELRFLDRHLN 60
Cdd:smart00595  42 EECKKRWKNLRDRYRRELKRLQNGKSGGGKKSKWEYFDRLSFLRPVIR 89
MADF_DNA_bdg pfam10545
Alcohol dehydrogenase transcription factor Myb/SANT-like; The myb/SANT-like domain in Adf-1 ...
 1-55 6.25e-07

Alcohol dehydrogenase transcription factor Myb/SANT-like; The myb/SANT-like domain in Adf-1 (MADF) is an approximately 80-amino-acid module that directs sequence specific DNA binding to a site consisting of multiple tri-nucleotide repeats. The MADF domain is found in one or more copies in eukaryotic and viral proteins and is often associated with the BESS domain. It is likely that the MADF domain is more closely related to the myb/SANT domain than it is to other HTH domains.


Pssm-ID: 463144  Cd Length: 84  Bit Score: 48.05  E-value: 6.25e-07
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 442632302    1 MMGDFLFENSCLEKCKRRWRQLRNDYTRWCNAdANRRRNGQRRLAYPLADELRFL 55
Cdd:pfam10545  31 EIAEELGSDVPVEDCKKRWKNLRDQYRRELRR-KRTNSGELYKSRWYYYEELSFL 84
COG5177 COG5177
Uncharacterized conserved protein [Function unknown];
93-229 6.05e-03

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227504 [Multi-domain]  Cd Length: 769  Bit Score: 40.45  E-value: 6.05e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442632302  93 QASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEK-------------LNDLEESDEPEKV 159
Cdd:COG5177  302 QYEQTIREIFADRATKLELDLQTVFESNMNRDTLDEYAPEGEDLRSDYDEDFEYdglttvriddhgfLPGREQTSKKAAV 381

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 442632302 160 PELDSFLQS------DNEDDECMDEEHLEDLEGFDFDLEQSNQEKELTPEKSLEKNNTDSTVSqPEFFVKQTRDPR 229
Cdd:COG5177  382 PKGTSFYQAkwaedeEEEDGQCNDEESTMSAIDDDDPKENDNEEVAGDEESAIDDNEGFEELS-PEEEERQLREFR 456
PRK05901 PRK05901
RNA polymerase sigma factor; Provisional
 74-230 8.52e-03

RNA polymerase sigma factor; Provisional


Pssm-ID: 235640 [Multi-domain]  Cd Length: 509  Bit Score: 39.98  E-value: 8.52e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442632302  74 SSDKDRDNNRDSEGVDHHAQASLKERASSTSKLVKEVKLASQVRKEKSSQDKRENWENPGDKQRSRKKSAEEKLNDLEES 153
Cdd:PRK05901  45 KKKTPEQIDQVLIFLSGMVKDTDDATESDIPKKKTKTAAKAAAAKAPAKKKLKDELDSSKKAEKKNALDKDDDLNYVKDI 124

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 442632302 154 DEPEKVPELDSFLQSDNEDDECMDEEHLEDLEGFDFDLEQSNQEKE-LTPEKSLEKNNTDSTVSQPEFFVKQTRDPRL 230
Cdd:PRK05901 125 DVLNQADDDDDDDDDDDLDDDDIDDDDDDEDDDEDDDDDDVDDEDEeKKEAKELEKLSDDDDFVWDEDDSEALRQARK 202
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH