NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|665402396|ref|NP_001286657|]
View 

uncharacterized protein Dmel_CG9313, isoform B [Drosophila melanogaster]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 413-640 1.40e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 56.96  E-value: 1.40e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 413 GVMCCDIHPTYPFLAViGLYDGNVAVYNLREDckEPLYVSRGvnckHGECVWQIKWgldMADGEVnFFSVSSDGRVFNWI 492
Cdd:cd00200   11 GVTCVAFSPDGKLLAT-GSGDGTIKVWDLETG--ELLRTLKG----HTGPVRDVAA---SADGTY-LASGSSDKTIRLWD 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 493 LMQNKL---------WVTTiITLYRENGLV------------DGPDGTKVTLKSG----GSCMVFHPvDNKIFLVGTECG 547
Cdd:cd00200   80 LETGECvrtltghtsYVSS-VAFSPDGRILssssrdktikvwDVETGKCLTTLRGhtdwVNSVAFSP-DGTFVASSSQDG 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 548 YIYKCSTAfSSKYLMTYYAHNMSVYRIDFNRfNSNIFVSCGADWMVKVWeDMRPDPL---FIFDLGaAVGDVKWAPySST 624
Cdd:cd00200  158 TIKLWDLR-TGKCVATLTGHTGEVNSVAFSP-DGEKLLSSSSDGTIKLW-DLSTGKClgtLRGHEN-GVNSVAFSP-DGY 232

                        250
                 ....*....|....*.
gi 665402396 625 VFAAVTTEGKVHVFDL 640
Cdd:cd00200  233 LLASGSEDGTIRVWDL 248
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 413-640 1.40e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 56.96  E-value: 1.40e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 413 GVMCCDIHPTYPFLAViGLYDGNVAVYNLREDckEPLYVSRGvnckHGECVWQIKWgldMADGEVnFFSVSSDGRVFNWI 492
Cdd:cd00200   11 GVTCVAFSPDGKLLAT-GSGDGTIKVWDLETG--ELLRTLKG----HTGPVRDVAA---SADGTY-LASGSSDKTIRLWD 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 493 LMQNKL---------WVTTiITLYRENGLV------------DGPDGTKVTLKSG----GSCMVFHPvDNKIFLVGTECG 547
Cdd:cd00200   80 LETGECvrtltghtsYVSS-VAFSPDGRILssssrdktikvwDVETGKCLTTLRGhtdwVNSVAFSP-DGTFVASSSQDG 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 548 YIYKCSTAfSSKYLMTYYAHNMSVYRIDFNRfNSNIFVSCGADWMVKVWeDMRPDPL---FIFDLGaAVGDVKWAPySST 624
Cdd:cd00200  158 TIKLWDLR-TGKCVATLTGHTGEVNSVAFSP-DGEKLLSSSSDGTIKLW-DLSTGKClgtLRGHEN-GVNSVAFSP-DGY 232

                        250
                 ....*....|....*.
gi 665402396 625 VFAAVTTEGKVHVFDL 640
Cdd:cd00200  233 LLASGSEDGTIRVWDL 248
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 557-597 1.91e-05

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 42.30  E-value: 1.91e-05
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|.
gi 665402396   557 SSKYLMTYYAHNMSVYRIDFNRfNSNIFVSCGADWMVKVWE 597
Cdd:smart00320   1 SGELLKTLKGHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
WD40 COG2319
WD40 repeat [General function prediction only];
426-641 3.19e-04

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 43.75  E-value: 3.19e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 426 LAVIGLYDGNVAVYNLREdcKEPLYVSRGvnckHGECVWQIKWGldmADGEVnFFSVSSDGRVFNWILMQNKLwvttIIT 505
Cdd:COG2319   92 LLASASADGTVRLWDLAT--GLLLRTLTG----HTGAVRSVAFS---PDGKT-LASGSADGTVRLWDLATGKL----LRT 157

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 506 LYRENGLVDG----PDGTkvTLKSGGScmvfhpvDNKIFLVGTECGyiykcstafssKYLMTYYAHNMSVYRIDFNRfNS 581
Cdd:COG2319  158 LTGHSGAVTSvafsPDGK--LLASGSD-------DGTVRLWDLATG-----------KLLRTLTGHTGAVRSVAFSP-DG 216

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 665402396 582 NIFVSCGADWMVKVWEDMRPDPLFIFDL-GAAVGDVKWAPYSSTVfAAVTTEGKVHVFDLN 641
Cdd:COG2319  217 KLLASGSADGTVRLWDLATGKLLRTLTGhSGSVRSVAFSPDGRLL-ASGSADGTVRLWDLA 276
WD40 pfam00400
WD domain, G-beta repeat;
558-597 3.77e-04

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 38.48  E-value: 3.77e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 665402396  558 SKYLMTYYAHNMSVYRIDFNrFNSNIFVSCGADWMVKVWE 597
Cdd:pfam00400   1 GKLLKTLEGHTGSVTSLAFS-PDGKLLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 413-640 1.40e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 56.96  E-value: 1.40e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 413 GVMCCDIHPTYPFLAViGLYDGNVAVYNLREDckEPLYVSRGvnckHGECVWQIKWgldMADGEVnFFSVSSDGRVFNWI 492
Cdd:cd00200   11 GVTCVAFSPDGKLLAT-GSGDGTIKVWDLETG--ELLRTLKG----HTGPVRDVAA---SADGTY-LASGSSDKTIRLWD 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 493 LMQNKL---------WVTTiITLYRENGLV------------DGPDGTKVTLKSG----GSCMVFHPvDNKIFLVGTECG 547
Cdd:cd00200   80 LETGECvrtltghtsYVSS-VAFSPDGRILssssrdktikvwDVETGKCLTTLRGhtdwVNSVAFSP-DGTFVASSSQDG 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 548 YIYKCSTAfSSKYLMTYYAHNMSVYRIDFNRfNSNIFVSCGADWMVKVWeDMRPDPL---FIFDLGaAVGDVKWAPySST 624
Cdd:cd00200  158 TIKLWDLR-TGKCVATLTGHTGEVNSVAFSP-DGEKLLSSSSDGTIKLW-DLSTGKClgtLRGHEN-GVNSVAFSP-DGY 232

                        250
                 ....*....|....*.
gi 665402396 625 VFAAVTTEGKVHVFDL 640
Cdd:cd00200  233 LLASGSEDGTIRVWDL 248
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 412-639 4.01e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 52.34  E-value: 4.01e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 412 CGVMCCDIHPTYPFLAViGLYDGNVAVYNLREdcKEPLYVSRGvnckHGECVWQIKWGLDmadgevNFFSVSS--DGRVf 489
Cdd:cd00200   94 SYVSSVAFSPDGRILSS-SSRDKTIKVWDVET--GKCLTTLRG----HTDWVNSVAFSPD------GTFVASSsqDGTI- 159

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 490 nwilmqnKLWVTTIITLYRenglvdgpdgtkvTL---KSGGSCMVFHPvDNKIFLVGTECG--YIYKCSTAFSskyLMTY 564
Cdd:cd00200  160 -------KLWDLRTGKCVA-------------TLtghTGEVNSVAFSP-DGEKLLSSSSDGtiKLWDLSTGKC---LGTL 215

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 665402396 565 YAHNMSVYRIDFNRfNSNIFVSCGADWMVKVWEDMRPDPLFIFDL-GAAVGDVKWAPySSTVFAAVTTEGKVHVFD 639
Cdd:cd00200  216 RGHENGVNSVAFSP-DGYLLASGSEDGTIRVWDLRTGECVQTLSGhTNSVTSLAWSP-DGKRLASGSADGTIRIWD 289
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 557-597 1.91e-05

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 42.30  E-value: 1.91e-05
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|.
gi 665402396   557 SSKYLMTYYAHNMSVYRIDFNRfNSNIFVSCGADWMVKVWE 597
Cdd:smart00320   1 SGELLKTLKGHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
WD40 COG2319
WD40 repeat [General function prediction only];
426-641 3.19e-04

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 43.75  E-value: 3.19e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 426 LAVIGLYDGNVAVYNLREdcKEPLYVSRGvnckHGECVWQIKWGldmADGEVnFFSVSSDGRVFNWILMQNKLwvttIIT 505
Cdd:COG2319   92 LLASASADGTVRLWDLAT--GLLLRTLTG----HTGAVRSVAFS---PDGKT-LASGSADGTVRLWDLATGKL----LRT 157

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 506 LYRENGLVDG----PDGTkvTLKSGGScmvfhpvDNKIFLVGTECGyiykcstafssKYLMTYYAHNMSVYRIDFNRfNS 581
Cdd:COG2319  158 LTGHSGAVTSvafsPDGK--LLASGSD-------DGTVRLWDLATG-----------KLLRTLTGHTGAVRSVAFSP-DG 216

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 665402396 582 NIFVSCGADWMVKVWEDMRPDPLFIFDL-GAAVGDVKWAPYSSTVfAAVTTEGKVHVFDLN 641
Cdd:COG2319  217 KLLASGSADGTVRLWDLATGKLLRTLTGhSGSVRSVAFSPDGRLL-ASGSADGTVRLWDLA 276
WD40 pfam00400
WD domain, G-beta repeat;
558-597 3.77e-04

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 38.48  E-value: 3.77e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 665402396  558 SKYLMTYYAHNMSVYRIDFNrFNSNIFVSCGADWMVKVWE 597
Cdd:pfam00400   1 GKLLKTLEGHTGSVTSLAFS-PDGKLLASGSDDGTVKVWD 39
WD40 COG2319
WD40 repeat [General function prediction only];
426-641 6.45e-04

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 42.98  E-value: 6.45e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 426 LAVIGLYDGNVAVYNLREDckEPLYVSRGvnckHGECVWQIKWGldmADGEVnFFSVSSDGRVfnwilmqnKLWvttiit 505
Cdd:COG2319  176 LLASGSDDGTVRLWDLATG--KLLRTLTG----HTGAVRSVAFS---PDGKL-LASGSADGTV--------RLW------ 231

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665402396 506 lyrenglvDGPDGTKVTLKSGGS----CMVFHPvDNKIFLVGTECGYIYKCSTAfSSKYLMTYYAHNMSVYRIDFNRfNS 581
Cdd:COG2319  232 --------DLATGKLLRTLTGHSgsvrSVAFSP-DGRLLASGSADGTVRLWDLA-TGELLRTLTGHSGGVNSVAFSP-DG 300

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 665402396 582 NIFVSCGADWMVKVWEDMRPDPLFIFD-LGAAVGDVKWAPYSSTVfAAVTTEGKVHVFDLN 641
Cdd:COG2319  301 KLLASGSDDGTVRLWDLATGKLLRTLTgHTGAVRSVAFSPDGKTL-ASGSDDGTVRLWDLA 360
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH