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Conserved domains on  [gi|1032528716|ref|NP_001314725|]
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uncharacterized protein LOC799792 [Danio rerio]

Protein Classification

THAP and DDE_Tnp_4 domain-containing protein( domain architecture ID 10659559)

protein containing domains THAP, HTH_Tnp_4, and DDE_Tnp_4

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 373-538 2.22e-41

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


:

Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 146.27  E-value: 2.22e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716 373 VDSFEICTQKGSSFKSSAHTFSHCKHHQTMKYLFGITPRGSISFISKGWEGCVSDKHVTENSGLLDQL----LPGDLVLA 448
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHHNTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALnlafGPGDYILA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716 449 DHGFEIghSGGLMCAELKITGGRrlLDAKDAVEKQQIAHLGVHVDRVTGCVRTKYIMLNGIVPVSMavpcegedMTFLDK 528
Cdd:pfam13359  81 DKGFPI--SDWLLVPYKKPGGAQ--LSPEEVEFNRRIASARIHVERVIGRLKGRFRILRGRLPISL--------PERLDK 148

                         170
                  ....*....|
gi 1032528716 529 IVTVCCALTN 538
Cdd:pfam13359 149 IVTVCCVLHN 158
THAP smart00980
The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It ...
 4-73 5.30e-06

The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


:

Pssm-ID: 214951  Cd Length: 80  Bit Score: 44.73  E-value: 5.30e-06
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716    4 RCSYGDCKTDERypeRVINIKFYPFPKpcRNLQKCLKWLRLCGGPNRQlnmdklrIHSKAKHIYVCSKHF 73
Cdd:smart00980   1 KCCVPGCGNRSK---KNPGLSFFRFPK--EDPELRKKWLENLGLPDDP-------NRKPKKRSRICSRHF 58
HTH_Tnp_4 super family cl16321
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
 291-343 5.43e-06

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


The actual alignment was detected with superfamily member pfam13613:

Pssm-ID: 463934  Cd Length: 53  Bit Score: 43.66  E-value: 5.43e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1032528716 291 HKLSPFQMLLLTLMRLKLNLPVQHIAYLFCVDQDTVSNTFKDTLGTMFQQLKS 343
Cdd:pfam13613   1 RKLSLEDQLLLTLVYLRLNLTFQDLAYRFGVSESTASRIFHKWLDLLAQRLPP 53
 
Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 373-538 2.22e-41

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 146.27  E-value: 2.22e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716 373 VDSFEICTQKGSSFKSSAHTFSHCKHHQTMKYLFGITPRGSISFISKGWEGCVSDKHVTENSGLLDQL----LPGDLVLA 448
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHHNTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALnlafGPGDYILA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716 449 DHGFEIghSGGLMCAELKITGGRrlLDAKDAVEKQQIAHLGVHVDRVTGCVRTKYIMLNGIVPVSMavpcegedMTFLDK 528
Cdd:pfam13359  81 DKGFPI--SDWLLVPYKKPGGAQ--LSPEEVEFNRRIASARIHVERVIGRLKGRFRILRGRLPISL--------PERLDK 148

                         170
                  ....*....|
gi 1032528716 529 IVTVCCALTN 538
Cdd:pfam13359 149 IVTVCCVLHN 158
THAP smart00980
The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It ...
 4-73 5.30e-06

The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 214951  Cd Length: 80  Bit Score: 44.73  E-value: 5.30e-06
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716    4 RCSYGDCKTDERypeRVINIKFYPFPKpcRNLQKCLKWLRLCGGPNRQlnmdklrIHSKAKHIYVCSKHF 73
Cdd:smart00980   1 KCCVPGCGNRSK---KNPGLSFFRFPK--EDPELRKKWLENLGLPDDP-------NRKPKKRSRICSRHF 58
HTH_Tnp_4 pfam13613
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
 291-343 5.43e-06

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


Pssm-ID: 463934  Cd Length: 53  Bit Score: 43.66  E-value: 5.43e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1032528716 291 HKLSPFQMLLLTLMRLKLNLPVQHIAYLFCVDQDTVSNTFKDTLGTMFQQLKS 343
Cdd:pfam13613   1 RKLSLEDQLLLTLVYLRLNLTFQDLAYRFGVSESTASRIFHKWLDLLAQRLPP 53
THAP pfam05485
THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a ...
 5-76 4.57e-05

THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion. It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 461662  Cd Length: 76  Bit Score: 41.75  E-value: 4.57e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1032528716   5 CSYGDCKTDERYPERvinIKFYPFPKpcrNLQKCLKWLRLCggpnrqlnmdKLRIHSKAKHIYVCSKHFVDG 76
Cdd:pfam05485   1 CSVPGCTNRKKKNPR---TSFHKFPK---DPERRKKWLNAC----------KRKDLPPPSNSYVCSLHFEEN 56
 
Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 373-538 2.22e-41

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 146.27  E-value: 2.22e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716 373 VDSFEICTQKGSSFKSSAHTFSHCKHHQTMKYLFGITPRGSISFISKGWEGCVSDKHVTENSGLLDQL----LPGDLVLA 448
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHHNTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALnlafGPGDYILA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716 449 DHGFEIghSGGLMCAELKITGGRrlLDAKDAVEKQQIAHLGVHVDRVTGCVRTKYIMLNGIVPVSMavpcegedMTFLDK 528
Cdd:pfam13359  81 DKGFPI--SDWLLVPYKKPGGAQ--LSPEEVEFNRRIASARIHVERVIGRLKGRFRILRGRLPISL--------PERLDK 148

                         170
                  ....*....|
gi 1032528716 529 IVTVCCALTN 538
Cdd:pfam13359 149 IVTVCCVLHN 158
THAP smart00980
The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It ...
 4-73 5.30e-06

The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 214951  Cd Length: 80  Bit Score: 44.73  E-value: 5.30e-06
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1032528716    4 RCSYGDCKTDERypeRVINIKFYPFPKpcRNLQKCLKWLRLCGGPNRQlnmdklrIHSKAKHIYVCSKHF 73
Cdd:smart00980   1 KCCVPGCGNRSK---KNPGLSFFRFPK--EDPELRKKWLENLGLPDDP-------NRKPKKRSRICSRHF 58
HTH_Tnp_4 pfam13613
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
 291-343 5.43e-06

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


Pssm-ID: 463934  Cd Length: 53  Bit Score: 43.66  E-value: 5.43e-06
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1032528716 291 HKLSPFQMLLLTLMRLKLNLPVQHIAYLFCVDQDTVSNTFKDTLGTMFQQLKS 343
Cdd:pfam13613   1 RKLSLEDQLLLTLVYLRLNLTFQDLAYRFGVSESTASRIFHKWLDLLAQRLPP 53
THAP pfam05485
THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a ...
 5-76 4.57e-05

THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion. It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 461662  Cd Length: 76  Bit Score: 41.75  E-value: 4.57e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1032528716   5 CSYGDCKTDERYPERvinIKFYPFPKpcrNLQKCLKWLRLCggpnrqlnmdKLRIHSKAKHIYVCSKHFVDG 76
Cdd:pfam05485   1 CSVPGCTNRKKKNPR---TSFHKFPK---DPERRKKWLNAC----------KRKDLPPPSNSYVCSLHFEEN 56
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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