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Conserved domains on  [gi|1063730688|ref|NP_001331317|]
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Isoprenylcysteine carboxyl methyltransferase (ICMT) family [Arabidopsis thaliana]

Protein Classification

phosphatidylethanolamine N-methyltransferase family domain-containing protein( domain architecture ID 229533)

phosphatidylethanolamine N-methyltransferase (PEMT) family domain-containing protein similar to Homo sapiens PEMT, which catalyzes the three sequential steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME), PMME to phosphatidyldimethylethanolamine (PDME), and PDME to phosphatidylcholine (PC)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ICMT super family cl21511
Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine ...
 82-174 2.16e-24

Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine o-methyltransferase (EC:2.1.1.100) family carry out carboxyl methylation of cleaved eukaryotic proteins that terminate in a CaaX motif. In Saccharomyces cerevisiae this methylation is carried out by Ste14p, an integral endoplasmic reticulum membrane protein. Ste14p is the founding member of the isoprenylcysteine carboxyl methyltransferase (ICMT) family, whose members share significant sequence homology.


The actual alignment was detected with superfamily member pfam04140:

Pssm-ID: 473892 [Multi-domain]  Cd Length: 95  Bit Score: 91.65  E-value: 2.16e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688  82 GLTMIILGEILRKTAIITAGRSFTHLIKIRREehHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNP--ISAIAFAVVV 159
Cdd:pfam04140   1 LLGLFLFGQILRKWAMVTLGRYWNHRVAILPD--HRLVTSGPYRYLRHPNYFGWFIWEIATQPLLCNAwyTALVFYALNA 78

                          90
                  ....*....|....*
gi 1063730688 160 WRFFAERIPYEEHYL 174
Cdd:pfam04140  79 WLLFSVRIRQEEKAL 93
 
Name Accession Description Interval E-value
ICMT pfam04140
Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine ...
 82-174 2.16e-24

Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine o-methyltransferase (EC:2.1.1.100) family carry out carboxyl methylation of cleaved eukaryotic proteins that terminate in a CaaX motif. In Saccharomyces cerevisiae this methylation is carried out by Ste14p, an integral endoplasmic reticulum membrane protein. Ste14p is the founding member of the isoprenylcysteine carboxyl methyltransferase (ICMT) family, whose members share significant sequence homology.


Pssm-ID: 427740 [Multi-domain]  Cd Length: 95  Bit Score: 91.65  E-value: 2.16e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688  82 GLTMIILGEILRKTAIITAGRSFTHLIKIRREehHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNP--ISAIAFAVVV 159
Cdd:pfam04140   1 LLGLFLFGQILRKWAMVTLGRYWNHRVAILPD--HRLVTSGPYRYLRHPNYFGWFIWEIATQPLLCNAwyTALVFYALNA 78

                          90
                  ....*....|....*
gi 1063730688 160 WRFFAERIPYEEHYL 174
Cdd:pfam04140  79 WLLFSVRIRQEEKAL 93
STE14 COG2020
Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein ...
 82-196 5.27e-23

Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441623 [Multi-domain]  Cd Length: 113  Bit Score: 88.68  E-value: 5.27e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688  82 GLTMIILGEILRKTAIITAGRSFTHLIKIRreEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWR 161
Cdd:COG2020     2 GLVLILLGLLLRLWAVLTLGRSWTTLVPPR--KAHRLVTTGPYRYVRHPMYLGFLLLLLGLALLLGSLLALLLALLLLLL 79

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1063730688 162 FFAeRIPYEEHYLKQFFGRQYVEYAQRVPSGVPFV 196
Cdd:COG2020    80 YVL-RIRREERRLRARFGEEYRAYAARVPRLIPRL 113
isopcys_mtase NF040696
protein-S-isoprenylcysteine O-methyltransferase;
101-196 1.45e-13

protein-S-isoprenylcysteine O-methyltransferase;


Pssm-ID: 468660  Cd Length: 190  Bit Score: 65.73  E-value: 1.45e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688 101 GRSFTHLIKIRreEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGR 180
Cdd:NF040696   97 GRNWSPSLEIR--EGHTLVTSGVYRYIRHPMYAAFWLWAIAQALLLPNWIAGLAGLVGFGILYFLRVPREEQMMLETFGD 174

                          90
                  ....*....|....*.
gi 1063730688 181 QYVEYAQRVPSGVPFV 196
Cdd:NF040696  175 EYRAYMQRTGRLIPKI 190
 
Name Accession Description Interval E-value
ICMT pfam04140
Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine ...
 82-174 2.16e-24

Isoprenylcysteine carboxyl methyltransferase (ICMT) family; The isoprenylcysteine o-methyltransferase (EC:2.1.1.100) family carry out carboxyl methylation of cleaved eukaryotic proteins that terminate in a CaaX motif. In Saccharomyces cerevisiae this methylation is carried out by Ste14p, an integral endoplasmic reticulum membrane protein. Ste14p is the founding member of the isoprenylcysteine carboxyl methyltransferase (ICMT) family, whose members share significant sequence homology.


Pssm-ID: 427740 [Multi-domain]  Cd Length: 95  Bit Score: 91.65  E-value: 2.16e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688  82 GLTMIILGEILRKTAIITAGRSFTHLIKIRREehHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNP--ISAIAFAVVV 159
Cdd:pfam04140   1 LLGLFLFGQILRKWAMVTLGRYWNHRVAILPD--HRLVTSGPYRYLRHPNYFGWFIWEIATQPLLCNAwyTALVFYALNA 78

                          90
                  ....*....|....*
gi 1063730688 160 WRFFAERIPYEEHYL 174
Cdd:pfam04140  79 WLLFSVRIRQEEKAL 93
STE14 COG2020
Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein ...
 82-196 5.27e-23

Protein-S-isoprenylcysteine O-methyltransferase Ste14 [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 441623 [Multi-domain]  Cd Length: 113  Bit Score: 88.68  E-value: 5.27e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688  82 GLTMIILGEILRKTAIITAGRSFTHLIKIRreEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWR 161
Cdd:COG2020     2 GLVLILLGLLLRLWAVLTLGRSWTTLVPPR--KAHRLVTTGPYRYVRHPMYLGFLLLLLGLALLLGSLLALLLALLLLLL 79

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 1063730688 162 FFAeRIPYEEHYLKQFFGRQYVEYAQRVPSGVPFV 196
Cdd:COG2020    80 YVL-RIRREERRLRARFGEEYRAYAARVPRLIPRL 113
isopcys_mtase NF040696
protein-S-isoprenylcysteine O-methyltransferase;
101-196 1.45e-13

protein-S-isoprenylcysteine O-methyltransferase;


Pssm-ID: 468660  Cd Length: 190  Bit Score: 65.73  E-value: 1.45e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688 101 GRSFTHLIKIRreEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGR 180
Cdd:NF040696   97 GRNWSPSLEIR--EGHTLVTSGVYRYIRHPMYAAFWLWAIAQALLLPNWIAGLAGLVGFGILYFLRVPREEQMMLETFGD 174

                          90
                  ....*....|....*.
gi 1063730688 181 QYVEYAQRVPSGVPFV 196
Cdd:NF040696  175 EYRAYMQRTGRLIPKI 190
PEMT pfam04191
Phospholipid methyltransferase; The S. cerevisiae phospholipid methyltransferase (EC:2.1.1.16) ...
 81-181 2.29e-07

Phospholipid methyltransferase; The S. cerevisiae phospholipid methyltransferase (EC:2.1.1.16) has a broad substrate specificity of unsaturated phospholipids.


Pssm-ID: 461218 [Multi-domain]  Cd Length: 105  Bit Score: 47.17  E-value: 2.29e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063730688  81 FGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVW 160
Cdd:pfam04191   5 LGLLLIALGLWLVLSSYRALGIFGTFYGDFFGILMDKLVTGGPYRYLNNPMYVGGTLGFLGLALITGSPAGLLLALLVLL 84

                          90       100
                  ....*....|....*....|.
gi 1063730688 161 RFFAERIPYEEHYLKQFFGRQ 181
Cdd:pfam04191  85 VYFIALKFVEEPHMAKIYGKR 105
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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