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Conserved domains on  [gi|1341269626|ref|NP_001347638|]
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apoptosis-enhancing nuclease isoform 1 [Mus musculus]

Protein Classification

3'-5' exonuclease family protein( domain architecture ID 1085)

3'-5' exonuclease family protein may cleave nucleotides one at a time from the end (exo) of a polynucleotide chain

CATH:  3.30.420.10
Gene Ontology:  GO:0003676
PubMed:  11988770|11222749
SCOP:  4000547

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
DnaQ_like_exo super family cl10012
DnaQ-like (or DEDD) 3'-5' exonuclease domain superfamily; The DnaQ-like exonuclease ...
 110-266 1.91e-88

DnaQ-like (or DEDD) 3'-5' exonuclease domain superfamily; The DnaQ-like exonuclease superfamily is a structurally conserved group of 3'-5' exonucleases, which catalyze the excision of nucleoside monophosphates at the DNA or RNA termini in the 3'-5' direction. It is also called the DEDD superfamily, after the four invariant acidic residues present in the catalytic site of its members. The superfamily consists of DNA- and RNA-processing enzymes such as the proofreading domains of DNA polymerases, other DNA exonucleases, RNase D, RNase T, Oligoribonuclease and RNA exonucleases (REX). The DnaQ-like exonuclease domain contains three conserved sequence motifs termed ExoI, ExoII and ExoIII, which are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The conservation patterns of the three motifs may vary among different subfamilies. DnaQ-like exonucleases are classified as DEDDy or DEDDh exonucleases depending on the variation of motif III as YX(3)D or HX(4)D, respectively. The significance of the motif differences is still unclear. Almost all RNase families in this superfamily are present only in eukaryotes and bacteria, but not in archaea, suggesting a later origin, which in some cases are accompanied by horizontal gene transfer.


The actual alignment was detected with superfamily member cd06149:

Pssm-ID: 447876  Cd Length: 157  Bit Score: 261.99  E-value: 1.91e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQGRVSELARCSVVSYSGDVLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVV 189
Cdd:cd06149     1 VAIDCEMVGTGPGGRESELARCSIVNYHGDVLYDKYIRPEGPVTDYRTRWSGIRRQHLVNATPFAVAQKEILKILKGKVV 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1341269626 190 VGHALHNDFQALKYVHPRSQTRDTTYVPnLLSQPSSL-IRTRVSLKDLALNLLHKKIQVGHQGHSSVEDAMTAMELYQ 266
Cdd:cd06149    81 VGHAIHNDFKALKYFHPKHMTRDTSTIP-LLNRKAGFpENCRVSLKVLAKRLLHRDIQVGRQGHSSVEDARATMELYK 157
 
Name Accession Description Interval E-value
ISG20 cd06149
DEDDh 3'-5' exonuclease domain of Interferon Stimulated Gene product of 20 kDa, and similar ...
 110-266 1.91e-88

DEDDh 3'-5' exonuclease domain of Interferon Stimulated Gene product of 20 kDa, and similar proteins; Interferon (IFN) Stimulated Gene product of 20 kDa (ISG20) is an IFN-induced antiviral exonuclease with a strong preference for single-stranded RNA and minor activity towards single-stranded DNA. It was also independently identified by its response to estrogen and was called HEM45 (human estrogen regulated transcript). ISG20 is a DEDDh-type DnaQ-like 3'-5' exonuclease containing three conserved sequence motifs termed ExoI, ExoII and ExoIII with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. ISG20 may be a major effector of innate immunity against pathogens including viruses, bacteria, and parasites. It is located in promyelocytic leukemia (PML) nuclear bodies, sites for oncogenic DNA viral transcription and replication. It may carry out its function by degrading viral RNAs as part of the IFN-regulated antiviral response.


Pssm-ID: 99852  Cd Length: 157  Bit Score: 261.99  E-value: 1.91e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQGRVSELARCSVVSYSGDVLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVV 189
Cdd:cd06149     1 VAIDCEMVGTGPGGRESELARCSIVNYHGDVLYDKYIRPEGPVTDYRTRWSGIRRQHLVNATPFAVAQKEILKILKGKVV 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1341269626 190 VGHALHNDFQALKYVHPRSQTRDTTYVPnLLSQPSSL-IRTRVSLKDLALNLLHKKIQVGHQGHSSVEDAMTAMELYQ 266
Cdd:cd06149    81 VGHAIHNDFKALKYFHPKHMTRDTSTIP-LLNRKAGFpENCRVSLKVLAKRLLHRDIQVGRQGHSSVEDARATMELYK 157
EXOIII smart00479
exonuclease domain in DNA-polymerase alpha and epsilon chain, ribonuclease T and other ...
 110-273 1.28e-38

exonuclease domain in DNA-polymerase alpha and epsilon chain, ribonuclease T and other exonucleases;


Pssm-ID: 214685 [Multi-domain]  Cd Length: 169  Bit Score: 134.74  E-value: 1.28e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626  110 VAIDCEMVGTGPQGrvSELARCSVVSYSGD---VLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKG 186
Cdd:smart00479   3 VVIDCETTGLDPGK--DEIIEIAAVDVDGGeiiEVFDTYVKPDRPITDYATEIHGITPEMLDDAPTFEEVLEELLEFLRG 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626  187 KV-VVGHALHNDFQALKYVHPR--------SQTRDT-TYVPNLLSQPSslirtRVSLKDLALNLLHKKIQVghqGHSSVE 256
Cdd:smart00479  81 RIlVAGNSAHFDLRFLKLEHPRlgikqppkLPVIDTlKLARATNPGLP-----KYSLKKLAKRLLLEVIQR---AHRALD 152

                          170
                   ....*....|....*..
gi 1341269626  257 DAMTAMELYQLVEVQWE 273
Cdd:smart00479 153 DARATAKLFKKLLERLE 169
RNase_T pfam00929
Exonuclease; This family includes a variety of exonuclease proteins, such as ribonuclease T ...
 110-265 8.23e-17

Exonuclease; This family includes a variety of exonuclease proteins, such as ribonuclease T and the epsilon subunit of DNA polymerase III.;


Pssm-ID: 395743 [Multi-domain]  Cd Length: 164  Bit Score: 76.62  E-value: 8.23e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQG-RVSELARCSVVSY--SGDVLYDKYIRPEMP--IVDYRTRWSGITRQHMHKAIPFQVAQKEILKLL 184
Cdd:pfam00929   1 VVIDLETTGLDPEKdEIIEIAAVVIDGGenEIGETFHTYVKPTRLpkLTDECTKFTGITQAMLDNKPSFEEVLEEFLEFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 185 -KGKVVVGH--------ALHNDFQALKYVHP-RSQTRDTTYvpnlLSQPSSLIRTRVSLKDLAlnllhKKIQVGHQG--H 252
Cdd:pfam00929  81 rKGNLLVAHnasfdvgfLRYDDKRFLKKPMPkLNPVIDTLI----LDKATYKELPGRSLDALA-----EKLGLEHIGraH 151

                         170
                  ....*....|...
gi 1341269626 253 SSVEDAMTAMELY 265
Cdd:pfam00929 152 RALDDARATAKLF 164
DnaQ COG0847
DNA polymerase III, epsilon subunit or related 3'-5' exonuclease [Replication, recombination ...
 110-266 2.06e-11

DNA polymerase III, epsilon subunit or related 3'-5' exonuclease [Replication, recombination and repair];


Pssm-ID: 440608 [Multi-domain]  Cd Length: 163  Bit Score: 61.35  E-value: 2.06e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGP-QGRVSELArcsVVSYSGDVLYDK---YIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLK 185
Cdd:COG0847     3 VVLDTETTGLDPaKDRIIEIG---AVKVDDGRIVETfhtLVNPERPIPPEATAIHGITDEDVADAPPFAEVLPELLEFLG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 186 GKVVVGH-------ALHNDFQALKYVHPRSQTRDTTyvpnLLSQpssliRTRVSLKDLALNLLHKKIQVGHQG-HSSVED 257
Cdd:COG0847    80 GAVLVAHnaafdlgFLNAELRRAGLPLPPFPVLDTL----RLAR-----RLLPGLPSYSLDALCERLGIPFDErHRALAD 150


                  ....*....
gi 1341269626 258 AMTAMELYQ 266
Cdd:COG0847   151 AEATAELFL 159
PRK08074 PRK08074
bifunctional ATP-dependent DNA helicase/DNA polymerase III subunit epsilon; Validated
 110-203 1.12e-05

bifunctional ATP-dependent DNA helicase/DNA polymerase III subunit epsilon; Validated


Pssm-ID: 236148 [Multi-domain]  Cd Length: 928  Bit Score: 47.25  E-value: 1.12e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGP-QG-RVSELArcSVVSYSGDVL--YDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLK 185
Cdd:PRK08074    6 VVVDLETTGNSPkKGdKIIQIA--AVVVEDGEILerFSSFVNPERPIPPFITELTGISEEMVKQAPLFEDVAPEIVELLE 83

                          90
                  ....*....|....*...
gi 1341269626 186 GKVVVGHALHNDFQALKY 203
Cdd:PRK08074   84 GAYFVAHNVHFDLNFLNE 101
 
Name Accession Description Interval E-value
ISG20 cd06149
DEDDh 3'-5' exonuclease domain of Interferon Stimulated Gene product of 20 kDa, and similar ...
 110-266 1.91e-88

DEDDh 3'-5' exonuclease domain of Interferon Stimulated Gene product of 20 kDa, and similar proteins; Interferon (IFN) Stimulated Gene product of 20 kDa (ISG20) is an IFN-induced antiviral exonuclease with a strong preference for single-stranded RNA and minor activity towards single-stranded DNA. It was also independently identified by its response to estrogen and was called HEM45 (human estrogen regulated transcript). ISG20 is a DEDDh-type DnaQ-like 3'-5' exonuclease containing three conserved sequence motifs termed ExoI, ExoII and ExoIII with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. ISG20 may be a major effector of innate immunity against pathogens including viruses, bacteria, and parasites. It is located in promyelocytic leukemia (PML) nuclear bodies, sites for oncogenic DNA viral transcription and replication. It may carry out its function by degrading viral RNAs as part of the IFN-regulated antiviral response.


Pssm-ID: 99852  Cd Length: 157  Bit Score: 261.99  E-value: 1.91e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQGRVSELARCSVVSYSGDVLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVV 189
Cdd:cd06149     1 VAIDCEMVGTGPGGRESELARCSIVNYHGDVLYDKYIRPEGPVTDYRTRWSGIRRQHLVNATPFAVAQKEILKILKGKVV 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1341269626 190 VGHALHNDFQALKYVHPRSQTRDTTYVPnLLSQPSSL-IRTRVSLKDLALNLLHKKIQVGHQGHSSVEDAMTAMELYQ 266
Cdd:cd06149    81 VGHAIHNDFKALKYFHPKHMTRDTSTIP-LLNRKAGFpENCRVSLKVLAKRLLHRDIQVGRQGHSSVEDARATMELYK 157
REX4_like cd06144
DEDDh 3'-5' exonuclease domain of RNA exonuclease 4, XPMC2, Interferon Stimulated Gene product ...
 110-266 2.32e-73

DEDDh 3'-5' exonuclease domain of RNA exonuclease 4, XPMC2, Interferon Stimulated Gene product of 20 kDa, and similar proteins; This subfamily is composed of RNA exonuclease 4 (REX4 or Rex4p), XPMC2, Interferon (IFN) Stimulated Gene product of 20 kDa (ISG20), and similar proteins. REX4 is involved in pre-rRNA processing. It controls the ratio between the two forms of 5.8S rRNA in yeast. XPMC2 is a Xenopus gene which was identified through its ability to correct a mitotic defect in fission yeast. The human homolog of XPMC2 (hPMC2) may be involved in angiotensin II-induced adrenal cell cycle progression and cell proliferation. ISG20 is an IFN-induced antiviral exonuclease with a strong preference for single-stranded RNA and minor activity towards single-stranded DNA. These proteins are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. REX proteins function in the processing and maturation of many RNA species, similar to the function of Escherchia coli RNase T.


Pssm-ID: 99847  Cd Length: 152  Bit Score: 223.55  E-value: 2.32e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQGRVSELARCSVVSYSGDVLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVV 189
Cdd:cd06144     1 VALDCEMVGVGPDGSESALARVSIVNEDGNVVYDTYVKPQEPVTDYRTAVSGIRPEHLKDAPDFEEVQKKVAELLKGRIL 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1341269626 190 VGHALHNDFQALKYVHPRSQTRDT-TYVPnlLSQPssLIRTRVSLKDLALNLLHKKIQVGHqgHSSVEDAMTAMELYQ 266
Cdd:cd06144    81 VGHALKNDLKVLKLDHPKKLIRDTsKYKP--LRKT--AKGKSPSLKKLAKQLLGLDIQEGE--HSSVEDARAAMRLYR 152
REX1_like cd06145
DEDDh 3'-5' exonuclease domain of RNA exonuclease 1, -3 and similar eukaryotic proteins; This ...
 110-264 1.21e-49

DEDDh 3'-5' exonuclease domain of RNA exonuclease 1, -3 and similar eukaryotic proteins; This subfamily is composed of RNA exonuclease 1 (REX1 or Rex1p), REX3 (or Rex3p), and similar eukaryotic proteins. In yeast, REX1 and REX3 are required for 5S rRNA and MRP (mitochondrial RNA processing) RNA maturation, respectively. They are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. REX1 is the major exonuclease responsible for pre-tRNA trail trimming and may also be involved in nuclear CCA turnover. REX proteins function in the processing and maturation of many RNA species, similar to the function of Escherichia coli RNase T.


Pssm-ID: 99848  Cd Length: 150  Bit Score: 162.65  E-value: 1.21e-49
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGpqgRVSELARCSVVSYSGDVLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIP-FQVAQKEILKLL-KGK 187
Cdd:cd06145     1 FALDCEMCYTT---DGLELTRVTVVDENGKVVLDELVKPDGEIVDYNTRFSGITEEMLENVTTtLEDVQKKLLSLIsPDT 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1341269626 188 VVVGHALHNDFQALKYVHPRsqTRDTTYVPNLLSQPssliRTRVSLKDLALNLLHKKIQVGHQGHSSVEDAMTAMEL 264
Cdd:cd06145    78 ILVGHSLENDLKALKLIHPR--VIDTAILFPHPRGP----PYKPSLKNLAKKYLGRDIQQGEGGHDSVEDARAALEL 148
DEDDh_RNase cd06137
DEDDh 3'-5' exonuclease domain of the eukaryotic exoribonucleases PAN2, RNA exonuclease (REX) ...
 110-266 1.66e-48

DEDDh 3'-5' exonuclease domain of the eukaryotic exoribonucleases PAN2, RNA exonuclease (REX)-1,-3, and -4, ISG20, and similar proteins; This group is composed of eukaryotic exoribonucleases that include PAN2, RNA exonuclease 1 (REX1 or Rex1p), REX3 (Rex3p), REX4 (or Rex4p), ISG20, and similar proteins. They are DEDDh-type DnaQ-like 3'-5' exonucleases containing three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. PAN2 is the catalytic subunit of poly(A) nuclease (PAN), a Pab1p-dependent 3'-5' exoribonuclease which plays an important role in the posttranscriptional maturation of pre-mRNAs. REX proteins are required for the processing and maturation of many RNA species, and ISG20 is an interferon-induced antiviral exonuclease with a strong preference for single-stranded RNA.


Pssm-ID: 99840  Cd Length: 161  Bit Score: 160.14  E-value: 1.66e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQGrvSELARCSVVSY-SGDVLYDKYIRPEMPIVDYRTRWSGITRQHM------HKAIP-FQVAQKEIL 181
Cdd:cd06137     1 VALDCEMVGLADGD--SEVVRISAVDVlTGEVLIDSLVRPSVRVTDWRTRFSGVTPADLeeaakaGKTIFgWEAARAALW 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 182 KLLKGK-VVVGHALHNDFQALKYVHPRsqTRDTTYVPNLLSQPSSLIRTRvSLKDLALNLLHKKIQVGHQGHSSVEDAMT 260
Cdd:cd06137    79 KFIDPDtILVGHSLQNDLDALRMIHTR--VVDTAILTREAVKGPLAKRQW-SLRTLCRDFLGLKIQGGGEGHDSLEDALA 155


                  ....*.
gi 1341269626 261 AMELYQ 266
Cdd:cd06137   156 AREVVL 161
EXOIII smart00479
exonuclease domain in DNA-polymerase alpha and epsilon chain, ribonuclease T and other ...
 110-273 1.28e-38

exonuclease domain in DNA-polymerase alpha and epsilon chain, ribonuclease T and other exonucleases;


Pssm-ID: 214685 [Multi-domain]  Cd Length: 169  Bit Score: 134.74  E-value: 1.28e-38
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626  110 VAIDCEMVGTGPQGrvSELARCSVVSYSGD---VLYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKG 186
Cdd:smart00479   3 VVIDCETTGLDPGK--DEIIEIAAVDVDGGeiiEVFDTYVKPDRPITDYATEIHGITPEMLDDAPTFEEVLEELLEFLRG 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626  187 KV-VVGHALHNDFQALKYVHPR--------SQTRDT-TYVPNLLSQPSslirtRVSLKDLALNLLHKKIQVghqGHSSVE 256
Cdd:smart00479  81 RIlVAGNSAHFDLRFLKLEHPRlgikqppkLPVIDTlKLARATNPGLP-----KYSLKKLAKRLLLEVIQR---AHRALD 152

                          170
                   ....*....|....*..
gi 1341269626  257 DAMTAMELYQLVEVQWE 273
Cdd:smart00479 153 DARATAKLFKKLLERLE 169
PAN2_exo cd06143
DEDDh 3'-5' exonuclease domain of the eukaryotic exoribonuclease PAN2; PAN2 is the catalytic ...
 110-266 1.80e-26

DEDDh 3'-5' exonuclease domain of the eukaryotic exoribonuclease PAN2; PAN2 is the catalytic subunit of poly(A) nuclease (PAN), a Pab1p-dependent 3'-5' exoribonuclease which plays an important role in the posttranscriptional maturation of pre-mRNAs. PAN catalyzes the deadenylation of poly(A) tails, which are initially synthesized to default lengths of 70 to 90, to mRNA-specific lengths of 55 to 71. Pab1p and PAN also play a role in the export and decay of mRNA. PAN2 contains a DEDDh-type DnaQ-like 3'-5' exonuclease domain with three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis.


Pssm-ID: 99846  Cd Length: 174  Bit Score: 103.08  E-value: 1.80e-26
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQGRVSE--------------LARCSVVSYSGD---VLY-DKYIRPEMPIVDYRTRWSGIT------RQ 165
Cdd:cd06143     1 VAIDAEFVKLKPEETEIRsdgtkstirpsqmsLARVSVVRGEGElegVPFiDDYISTTEPVVDYLTRFSGIKpgdldpKT 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 166 HMHKAIPFQVAQKEiLKLL--KGKVVVGHALHNDFQALKYVHPRSQTRDTTyvpNLLSQPSsliRTRVSLKDLALNLLHK 243
Cdd:cd06143    81 SSKNLTTLKSAYLK-LRLLvdLGCIFVGHGLAKDFRVINIQVPKEQVIDTV---ELFHLPG---QRKLSLRFLAWYLLGE 153

                         170       180
                  ....*....|....*....|...
gi 1341269626 244 KIQVGhqGHSSVEDAMTAMELYQ 266
Cdd:cd06143   154 KIQSE--THDSIEDARTALKLYR 174
RNase_T pfam00929
Exonuclease; This family includes a variety of exonuclease proteins, such as ribonuclease T ...
 110-265 8.23e-17

Exonuclease; This family includes a variety of exonuclease proteins, such as ribonuclease T and the epsilon subunit of DNA polymerase III.;


Pssm-ID: 395743 [Multi-domain]  Cd Length: 164  Bit Score: 76.62  E-value: 8.23e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGPQG-RVSELARCSVVSY--SGDVLYDKYIRPEMP--IVDYRTRWSGITRQHMHKAIPFQVAQKEILKLL 184
Cdd:pfam00929   1 VVIDLETTGLDPEKdEIIEIAAVVIDGGenEIGETFHTYVKPTRLpkLTDECTKFTGITQAMLDNKPSFEEVLEEFLEFL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 185 -KGKVVVGH--------ALHNDFQALKYVHP-RSQTRDTTYvpnlLSQPSSLIRTRVSLKDLAlnllhKKIQVGHQG--H 252
Cdd:pfam00929  81 rKGNLLVAHnasfdvgfLRYDDKRFLKKPMPkLNPVIDTLI----LDKATYKELPGRSLDALA-----EKLGLEHIGraH 151

                         170
                  ....*....|...
gi 1341269626 253 SSVEDAMTAMELY 265
Cdd:pfam00929 152 RALDDARATAKLF 164
DEDDh cd06127
DEDDh 3'-5' exonuclease domain family; DEDDh exonucleases, part of the DnaQ-like (or DEDD) ...
 110-266 8.15e-14

DEDDh 3'-5' exonuclease domain family; DEDDh exonucleases, part of the DnaQ-like (or DEDD) exonuclease superfamily, catalyze the excision of nucleoside monophosphates at the DNA or RNA termini in the 3'-5' direction. These proteins contain four invariant acidic residues in three conserved sequence motifs termed ExoI, ExoII and ExoIII. DEDDh exonucleases are classified as such because of the presence of specific Hx(4)D conserved pattern at the ExoIII motif. The four conserved acidic residues are clustered around the active site and serve as ligands for the two metal ions required for catalysis. Most DEDDh exonucleases are the proofreading subunits (epsilon) or domains of bacterial DNA polymerase III, the main replicating enzyme in bacteria, which functions as the chromosomal replicase. Other members include other DNA and RNA exonucleases such as RNase T, Oligoribonuclease, and RNA exonuclease (REX), among others.


Pssm-ID: 176648 [Multi-domain]  Cd Length: 159  Bit Score: 68.10  E-value: 8.15e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGP-QGRVSELArcsVVSYSGDVL----YDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLL 184
Cdd:cd06127     1 VVFDTETTGLDPkKDRIIEIG---AVKVDGGIEiverFETLVNPGRPIPPEATAIHGITDEMLADAPPFEEVLPEFLEFL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 185 KGKVVVGHALHNDFQALKYVHPRSQTR-------DTtyvpnlLSQPSSLIRTRVSLKDLALNLLHKKIQVgHQGHSSVED 257
Cdd:cd06127    78 GGRVLVAHNASFDLRFLNRELRRLGGPplpnpwiDT------LRLARRLLPGLRSHRLGLLLAERYGIPL-EGAHRALAD 150


                  ....*....
gi 1341269626 258 AMTAMELYQ 266
Cdd:cd06127   151 ALATAELLL 159
DnaQ COG0847
DNA polymerase III, epsilon subunit or related 3'-5' exonuclease [Replication, recombination ...
 110-266 2.06e-11

DNA polymerase III, epsilon subunit or related 3'-5' exonuclease [Replication, recombination and repair];


Pssm-ID: 440608 [Multi-domain]  Cd Length: 163  Bit Score: 61.35  E-value: 2.06e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGP-QGRVSELArcsVVSYSGDVLYDK---YIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLK 185
Cdd:COG0847     3 VVLDTETTGLDPaKDRIIEIG---AVKVDDGRIVETfhtLVNPERPIPPEATAIHGITDEDVADAPPFAEVLPELLEFLG 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 186 GKVVVGH-------ALHNDFQALKYVHPRSQTRDTTyvpnLLSQpssliRTRVSLKDLALNLLHKKIQVGHQG-HSSVED 257
Cdd:COG0847    80 GAVLVAHnaafdlgFLNAELRRAGLPLPPFPVLDTL----RLAR-----RLLPGLPSYSLDALCERLGIPFDErHRALAD 150


                  ....*....
gi 1341269626 258 AMTAMELYQ 266
Cdd:COG0847   151 AEATAELFL 159
PRK08074 PRK08074
bifunctional ATP-dependent DNA helicase/DNA polymerase III subunit epsilon; Validated
 110-203 1.12e-05

bifunctional ATP-dependent DNA helicase/DNA polymerase III subunit epsilon; Validated


Pssm-ID: 236148 [Multi-domain]  Cd Length: 928  Bit Score: 47.25  E-value: 1.12e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGP-QG-RVSELArcSVVSYSGDVL--YDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLK 185
Cdd:PRK08074    6 VVVDLETTGNSPkKGdKIIQIA--AVVVEDGEILerFSSFVNPERPIPPFITELTGISEEMVKQAPLFEDVAPEIVELLE 83

                          90
                  ....*....|....*...
gi 1341269626 186 GKVVVGHALHNDFQALKY 203
Cdd:PRK08074   84 GAYFVAHNVHFDLNFLNE 101
PolC COG2176
DNA polymerase III, alpha subunit (gram-positive type) [Replication, recombination and repair]; ...
 110-192 3.19e-04

DNA polymerase III, alpha subunit (gram-positive type) [Replication, recombination and repair];


Pssm-ID: 441779 [Multi-domain]  Cd Length: 181  Bit Score: 40.90  E-value: 3.19e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1341269626 110 VAIDCEMVGTGP-QGRVSELArcSVVSYSGDVL--YDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKG 186
Cdd:COG2176    11 VVFDLETTGLSPkKDEIIEIG--AVKVENGEIVdrFSTLVNPGRPIPPFITELTGITDEMVADAPPFEEVLPEFLEFLGD 88


                  ....*.
gi 1341269626 187 KVVVGH 192
Cdd:COG2176    89 AVLVAH 94
polC PRK00448
DNA polymerase III PolC; Validated
 142-192 7.05e-03

DNA polymerase III PolC; Validated


Pssm-ID: 234767 [Multi-domain]  Cd Length: 1437  Bit Score: 38.28  E-value: 7.05e-03
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|.
gi 1341269626  142 YDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVVVGH 192
Cdd:PRK00448   455 FEFFIKPGHPLSAFTTELTGITDDMVKDAPSIEEVLPKFKEFCGDSILVAH 505
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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