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Conserved domains on  [gi|1845978735|ref|NP_001369991|]
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Ubiquitin thioesterase otubain-like [Caenorhabditis elegans]

Protein Classification

OTU family ubiquitin thioesterase( domain architecture ID 10563197)

OTU family ubiquitin thioesterase catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin

EC:  3.4.19.12
Gene Ontology:  GO:0004843
MEROPS:  C65
SCOP:  4004307

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Peptidase_C65 pfam10275
Peptidase C65 Otubain; This family of proteins conserved from plants to humans is a highly ...
36-273 9.89e-117

Peptidase C65 Otubain; This family of proteins conserved from plants to humans is a highly specific ubiquitin iso-peptidase that removes ubiquitin from proteins. The modification of cellular proteins by ubiquitin (Ub) is an important event that underlies protein stability and function in eukaryote being a dynamic and reversible process. Otubain carries several key conserved domains: (i) the OTU (ovarian tumour domain) in which there is an active cysteine protease triad (ii) a nuclear localization signal, (iii) a Ub interaction motif (UIM)-like motif phi-xx-A-xxxs-xx-Ac (where phi indicates an aromatic amino acid, x indicates any amino acid and Ac indicates an acidic amino acid), (iv) a Ub-associated (UBA)-like domain and (v) the LxxLL motif.


:

Pssm-ID: 431191 [Multi-domain]  Cd Length: 240  Bit Score: 335.02  E-value: 9.89e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  36 DEQKSVPLVATLAPFSILCAEYDNEtSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKFIASS 115
Cdd:pfam10275   1 EEEAQGPLVSEKGPLSALEKEYAKA-DPIYLQKIQDLSEKYSGIRRTRGDGNCFYRAFGFSYLELLLESKDEIDRFKARV 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 116 RDWTRTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHT-EEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDE 194
Cdd:pfam10275  80 ESLKEALVALGFDEDTFEDFCDAFLELLKKVEDGVSTsESELLQAFNDQETSDYIVYFLRLLTSAYLKTHADEYEPFIDG 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 195 GMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHYDIPSDD--QQIAPEITLLYRPGHYDVIY 272
Cdd:pfam10275 160 GGTVEEFCQQEVEPMNKEADHLQIIALAEALGVPVRVEYLDRSAEGNTVNHHDFPGEDdtEEQAPFITLLYRPGHYDILY 239

                  .
gi 1845978735 273 K 273
Cdd:pfam10275 240 K 240
 
Name Accession Description Interval E-value
Peptidase_C65 pfam10275
Peptidase C65 Otubain; This family of proteins conserved from plants to humans is a highly ...
36-273 9.89e-117

Peptidase C65 Otubain; This family of proteins conserved from plants to humans is a highly specific ubiquitin iso-peptidase that removes ubiquitin from proteins. The modification of cellular proteins by ubiquitin (Ub) is an important event that underlies protein stability and function in eukaryote being a dynamic and reversible process. Otubain carries several key conserved domains: (i) the OTU (ovarian tumour domain) in which there is an active cysteine protease triad (ii) a nuclear localization signal, (iii) a Ub interaction motif (UIM)-like motif phi-xx-A-xxxs-xx-Ac (where phi indicates an aromatic amino acid, x indicates any amino acid and Ac indicates an acidic amino acid), (iv) a Ub-associated (UBA)-like domain and (v) the LxxLL motif.


Pssm-ID: 431191 [Multi-domain]  Cd Length: 240  Bit Score: 335.02  E-value: 9.89e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  36 DEQKSVPLVATLAPFSILCAEYDNEtSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKFIASS 115
Cdd:pfam10275   1 EEEAQGPLVSEKGPLSALEKEYAKA-DPIYLQKIQDLSEKYSGIRRTRGDGNCFYRAFGFSYLELLLESKDEIDRFKARV 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 116 RDWTRTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHT-EEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDE 194
Cdd:pfam10275  80 ESLKEALVALGFDEDTFEDFCDAFLELLKKVEDGVSTsESELLQAFNDQETSDYIVYFLRLLTSAYLKTHADEYEPFIDG 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 195 GMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHYDIPSDD--QQIAPEITLLYRPGHYDVIY 272
Cdd:pfam10275 160 GGTVEEFCQQEVEPMNKEADHLQIIALAEALGVPVRVEYLDRSAEGNTVNHHDFPGEDdtEEQAPFITLLYRPGHYDILY 239

                  .
gi 1845978735 273 K 273
Cdd:pfam10275 240 K 240
Otubain_C65 cd22749
Otubain subfamily of ubiquitin thioesterases; The otubain subfamily is composed of otubain-1 ...
43-272 1.64e-83

Otubain subfamily of ubiquitin thioesterases; The otubain subfamily is composed of otubain-1 (also called ubiquitin thioesterase OTUB1 or OTU domain-containing ubiquitin aldehyde-binding protein 1), otubain-2 (also called ubiquitin thioesterase OTUB2 or OTU domain-containing ubiquitin aldehyde-binding protein 2), and similar proteins. They function as deubiquitylases (DUBs)/ubiquitin thioesterases (EC 3.4.19.12). OTUB1 can specifically remove 'Lys-48'-linked conjugated ubiquitin from protein substrates, while OTUB2 mediates the deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. The otubain subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. Members of this subfamily are classified as family C65 cysteine proteases by MEROPS.


Pssm-ID: 438586 [Multi-domain]  Cd Length: 232  Bit Score: 250.33  E-value: 1.64e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  43 LVATLAPFSILCAEYdnETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIML--KDRARLEKFIASSRDWTR 120
Cdd:cd22749     1 LVGEKEPLSALAEEY--AGNPIFLQKIKELKKKYSGFRRVRGDGNCFYRAFAFSYLELLLknQDPAELERLLARLESLKN 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 121 TLVELGFPDWTCTDFCDFFIEFLEKIHSGV---HTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMT 197
Cdd:cd22749    79 LLEALGFEELVFEDFYEEFLELLKKLRNSKereLTEEELLELFNDEETSNYIVVFLRLLTSAYLKTNADDYEPFLFEGMS 158
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1845978735 198 VAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAApNGGWHYDIPSDDQQIAPEITLLYRPGHYDVIY 272
Cdd:cd22749   159 VEEFCEREVEPMGKEADHLQITALANALGVPVRVEYLDRSAG-GEVNFHEFPPEDSDSLPVITLLYRPGHYDILY 232
 
Name Accession Description Interval E-value
Peptidase_C65 pfam10275
Peptidase C65 Otubain; This family of proteins conserved from plants to humans is a highly ...
36-273 9.89e-117

Peptidase C65 Otubain; This family of proteins conserved from plants to humans is a highly specific ubiquitin iso-peptidase that removes ubiquitin from proteins. The modification of cellular proteins by ubiquitin (Ub) is an important event that underlies protein stability and function in eukaryote being a dynamic and reversible process. Otubain carries several key conserved domains: (i) the OTU (ovarian tumour domain) in which there is an active cysteine protease triad (ii) a nuclear localization signal, (iii) a Ub interaction motif (UIM)-like motif phi-xx-A-xxxs-xx-Ac (where phi indicates an aromatic amino acid, x indicates any amino acid and Ac indicates an acidic amino acid), (iv) a Ub-associated (UBA)-like domain and (v) the LxxLL motif.


Pssm-ID: 431191 [Multi-domain]  Cd Length: 240  Bit Score: 335.02  E-value: 9.89e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  36 DEQKSVPLVATLAPFSILCAEYDNEtSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKFIASS 115
Cdd:pfam10275   1 EEEAQGPLVSEKGPLSALEKEYAKA-DPIYLQKIQDLSEKYSGIRRTRGDGNCFYRAFGFSYLELLLESKDEIDRFKARV 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 116 RDWTRTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHT-EEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDE 194
Cdd:pfam10275  80 ESLKEALVALGFDEDTFEDFCDAFLELLKKVEDGVSTsESELLQAFNDQETSDYIVYFLRLLTSAYLKTHADEYEPFIDG 159
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 195 GMTVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHYDIPSDD--QQIAPEITLLYRPGHYDVIY 272
Cdd:pfam10275 160 GGTVEEFCQQEVEPMNKEADHLQIIALAEALGVPVRVEYLDRSAEGNTVNHHDFPGEDdtEEQAPFITLLYRPGHYDILY 239

                  .
gi 1845978735 273 K 273
Cdd:pfam10275 240 K 240
Otubain_C65 cd22749
Otubain subfamily of ubiquitin thioesterases; The otubain subfamily is composed of otubain-1 ...
43-272 1.64e-83

Otubain subfamily of ubiquitin thioesterases; The otubain subfamily is composed of otubain-1 (also called ubiquitin thioesterase OTUB1 or OTU domain-containing ubiquitin aldehyde-binding protein 1), otubain-2 (also called ubiquitin thioesterase OTUB2 or OTU domain-containing ubiquitin aldehyde-binding protein 2), and similar proteins. They function as deubiquitylases (DUBs)/ubiquitin thioesterases (EC 3.4.19.12). OTUB1 can specifically remove 'Lys-48'-linked conjugated ubiquitin from protein substrates, while OTUB2 mediates the deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. The otubain subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. Members of this subfamily are classified as family C65 cysteine proteases by MEROPS.


Pssm-ID: 438586 [Multi-domain]  Cd Length: 232  Bit Score: 250.33  E-value: 1.64e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  43 LVATLAPFSILCAEYdnETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIML--KDRARLEKFIASSRDWTR 120
Cdd:cd22749     1 LVGEKEPLSALAEEY--AGNPIFLQKIKELKKKYSGFRRVRGDGNCFYRAFAFSYLELLLknQDPAELERLLARLESLKN 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 121 TLVELGFPDWTCTDFCDFFIEFLEKIHSGV---HTEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMT 197
Cdd:cd22749    79 LLEALGFEELVFEDFYEEFLELLKKLRNSKereLTEEELLELFNDEETSNYIVVFLRLLTSAYLKTNADDYEPFLFEGMS 158
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1845978735 198 VAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAApNGGWHYDIPSDDQQIAPEITLLYRPGHYDVIY 272
Cdd:cd22749   159 VEEFCEREVEPMGKEADHLQITALANALGVPVRVEYLDRSAG-GEVNFHEFPPEDSDSLPVITLLYRPGHYDILY 232
OTUB1 cd22763
Ubiquitin Thioesterase Otubain-1; Otubain-1 is also called ubiquitin thioesterase OTUB1, ...
43-272 1.15e-71

Ubiquitin Thioesterase Otubain-1; Otubain-1 is also called ubiquitin thioesterase OTUB1, deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, or ubiquitin-specific-processing protease OTUB1. It is a deubiquitylase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that can specifically remove 'Lys-48'-linked conjugated ubiquitin from protein substrates. It is also capable of cleaving NEDD8 (neural-precursor-cell-expressed developmentally down-regulated 8), but not SUMO (small ubiquitin-related modifier) 1/2/3 and ISG15 (interferon-stimulated gene 15) conjugates. In addition, OTUB1 inhibits the DNA damage response independently of its catalytic activity by blocking ubiquitin transfer onto protein substrates via sequestration of E2 ubiquitin-conjugating enzymes. It also regulates many cancer-associated signaling pathways including MAPK, ERa, epithelial-mesenchymal transition (EMT), RHOa, mTORC1, FOXM1 and P53 to promote tumor cell survival, proliferation, invasiveness and therapeutic resistance. OTUB1 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C65 cysteine protease by MEROPS.


Pssm-ID: 438600  Cd Length: 224  Bit Score: 219.75  E-value: 1.15e-71
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  43 LVATLAPFSILCAEYDNEtSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKF---IASSRDwt 119
Cdd:cd22763     1 LVSEKEDLSVLEKEYAED-DPIYQAKIKDLKKKYSYIRRTRPDGNCFYRAFGFAYLESLLDDPEELQRFkevAAKSKD-- 77
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 120 rTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHTEEaVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVA 199
Cdd:cd22763    78 -ELVSLGFPSFTIEDFHDTFMEVLEKVEKGTSVEE-LLEIFNDQGTSDYLVVYLRLLTSGYLQKEADFFQNFIEGGRSVK 155
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1845978735 200 QYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGWHyDIPSDDQqiaPEITLLYRPGHYDVIY 272
Cdd:cd22763   156 EFCSQEVEPMYKESDHIHIIALTSALGVSVRVEYMDRGEGGTVNPH-DFPEGSE---PRIHLLYRPGHYDILY 224
AtOTU1-like cd22765
Arabidopsis thaliana Deubiquitinating enzyme OTU1 and similar plant proteins; This group ...
42-272 6.94e-60

Arabidopsis thaliana Deubiquitinating enzyme OTU1 and similar plant proteins; This group contains plant otibain-like proteins including Oryza sativa Japonica group otubain-like deubiquitinase and Arabidopsis thaliana deubiquitinating enzyme OTU1 (AtOTU1), also called OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 1 or OTU domain-containing protein 1. It is a deubiquitylase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that mediates the deubiquitination of protein substrates and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. AtOTU1 shows a preference for Met-1 and 'Lys-48' over 'Lys-63'-linked ubiquitin tetramers as substrates. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C65 cysteine protease by MEROPS.


Pssm-ID: 438602  Cd Length: 247  Bit Score: 190.65  E-value: 6.94e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  42 PLVATLAPFSILCAEYDNeTSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIML--KDRARLEKFIASSRDWT 119
Cdd:cd22765     1 PYVGDKEPLSALAAEYQS-GSPVFVAKIESLGETYGAIRRTRGDGNCFFRSFMFGYLEHLLetQDGAEVRRVLKRIEQCK 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 120 RTLVELGFPDWTCTDFCDFFIEFLEKIHSGVHTEEAVYTILN---DDGSANYILMFFRLITSAFLKQNSEEYAPFIdEGM 196
Cdd:cd22765    80 KKLVDLGYQELVFEDAMEILVEQLESIGQGDEESISIETLLEnmrDDMVSNYVVMFLRFVTSAEIQRRADFFEPFI-MGL 158
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 197 ---TVAQYCEQEIEPMWKDADHLAINSLIKAAGTRVRIEYMDRTAAPNGGW--------HYD-IPSD-DQQIAPEITLLY 263
Cdd:cd22765   159 snmTVEQFCRRSVEPMGEESDHVHIVALTDALQVPIRVVYLDRSSCDGAGGgaggvevnHHDfVPEGcPAAGRPRVHLLY 238

                  ....*....
gi 1845978735 264 RPGHYDVIY 272
Cdd:cd22765   239 RPGHYDILY 247
OTUB2 cd22764
Ubiquitin Thioesterase Otubain-2; Otubain-2 is also called ubiquitin thioesterase OTUB2, ...
58-272 2.64e-39

Ubiquitin Thioesterase Otubain-2; Otubain-2 is also called ubiquitin thioesterase OTUB2, deubiquitinating enzyme OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, or ubiquitin-specific-processing protease OTUB2. It is a deubiquitylase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that mediates the deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. OTUB2 plays a role in DNA double-strand break (DSB) response (DDR); it enhances RNF8-mediated ubiquitination in an early phase of the DDR and promotes faster DSB repair but suppresses homologous recombination. It also functions as a cancer stemness and metastasis-promoting factor that deubiquitinates and activates the transcriptional regulators YAP/TAZ, which play important roles in development, physiology, and tumorigenesis and are negatively controlled by the Hippo pathway. OTUB2 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C65 cysteine protease by MEROPS.


Pssm-ID: 438601  Cd Length: 222  Bit Score: 136.74  E-value: 2.64e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735  58 DNETSAAFLSKATELSEVYGEIRYIRGDGNCFYRAILVGLIEIMLKDRARLEKF----IASSRDwtrtLVELGFPDWTCT 133
Cdd:cd22764    14 EHPENPIYQRKLKDLSKRYASIRKTRGDGNCFYRALAFAYLESLLGNSREIQKFketvLQSKNE----LLAAGFEEHRFR 89
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 134 DFCDFFIEFLEKIHSGVHtEEAVYTILNDDGSANYILMFFRLITSAFLKQNSEEYAPFIDEGMTVAQYCEQEIEPMWKDA 213
Cdd:cd22764    90 NLFNTFVSVVELVEADGS-GSSLLKAFNDQTTSDSIVQYLRLLTSAFLQNRADFFQHFVEEGMNIKDFCTQEVEPMAMEC 168
                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 214 DHLAINSLIKAAGTRVRIEYMDRT-AAPNggwHYDIPSDDQqiaPEITLLYRPGHYDVIY 272
Cdd:cd22764   169 DHIQITALSQALGIPLQVEYVDEMdTALN---HHIFPEGAE---PSVYLLYKTSHYNILY 222
OTU_plant_OTU7-like cd22771
OTU (ovarian tumor) domain of Arabidopsis thaliana deubiquitinating enzyme OTU7 and similar ...
180-269 8.29e-06

OTU (ovarian tumor) domain of Arabidopsis thaliana deubiquitinating enzyme OTU7 and similar proteins; Arabidopsis thaliana deubiquitinating enzyme OTU7, also called OTU domain-containing protein 7, is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that shows a preference for 'Lys-63' over 'Lys-48' over 'Met-1'-linked ubiquitin (UB) tetramers as substrates. DUBs catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. OTU7 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438608 [Multi-domain]  Cd Length: 124  Bit Score: 44.08  E-value: 8.29e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1845978735 180 FLKQNSEEYAPFIDEGMTVAQYCEQeiepMWKD---ADHLAINSLIKAAGTRVRIEYMDRTAapnggwhYDIPSDDQQIA 256
Cdd:cd22771    39 YMEAHEEDFEPFFEDDETFEDYVSR----MREDgtwGGNLELQAASLVYRVNIVVHQLGQPR-------WEIENFPDKGA 107
                          90
                  ....*....|....
gi 1845978735 257 PEITLLYRPG-HYD 269
Cdd:cd22771   108 RTIHLSYHDGeHYN 121
OTU_CeDUB-like cd22755
OTU (ovarian tumor) domain of Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and ...
78-93 1.19e-03

OTU (ovarian tumor) domain of Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and OTU domains, and similar proteins; This subfamily is composed of mostly uncharacterized proteins containing an OTU domain, similar to Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and OTU domains. OTU domain-containing proteins function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438592 [Multi-domain]  Cd Length: 132  Bit Score: 38.40  E-value: 1.19e-03
                          10
                  ....*....|....*.
gi 1845978735  78 EIRYIRGDGNCFYRAI 93
Cdd:cd22755     2 KTIKIVGDGNCFFRAL 17
OTU cd22744
OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved ...
78-97 4.75e-03

OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation.


Pssm-ID: 438581 [Multi-domain]  Cd Length: 128  Bit Score: 36.26  E-value: 4.75e-03
                          10        20
                  ....*....|....*....|
gi 1845978735  78 EIRYIRGDGNCFYRAILVGL 97
Cdd:cd22744     1 RVVDVPGDGNCLFRALAHAL 20
OTU_OTUD3 cd22770
OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; OTU ...
65-128 5.64e-03

OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; OTU domain-containing protein 3 (OTUD3) is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It is an acetylation-dependent deubiquitinase that restricts innate antiviral immune signaling. It directly hydrolyzes lysine 63 (Lys63)-linked polyubiquitination of MAVS (mitochondrial antiviral-signaling protein) and shuts off innate antiviral immune response. OTUD3 can elicit tumor-suppressing or tumor-promoting activities in a cell- and tissue-dependent manner. It is a DUB for PTEN (phosphatase and tension homologue deleted on chromosome 10); the OTUD3-PTEN signaling axis plays a critical role in suppression of breast tumorigenesis. OTUD3 is also a DUB for glucose-regulated protein GRP78, stabilizing it and promoting lung tumorigenesis. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438607 [Multi-domain]  Cd Length: 145  Bit Score: 36.49  E-value: 5.64e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1845978735  65 FLSKATELSEVYGEIRYIRGDGNCFYRAI---LVG-----------LIEIMLKDRARLEKFIASSRDWTRTLVELGFP 128
Cdd:cd22770     2 FVSFANQLQALGLKLRDIPGDGNCLFRALgdqLEGhsrnhlkhrqeTVQYMIEHREDFEPFVEDDVPFDKHVANLSKP 79
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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