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Conserved domains on  [gi|1972256940|ref|NP_001379045|]
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FeS cluster biogenesis domain-containing protein [Caenorhabditis elegans]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 27-130 3.13e-38

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 124.88  E-value: 3.13e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  27 IKVTNKAASRLKEVVDNGER----LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGATVDF 102
Cdd:COG0316     2 ITLTDAAAKRIKRLLAKEGNpglgLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQ--DGVKVVVDPKSLPYLDGTEIDY 79

                          90       100
                  ....*....|....*....|....*...
gi 1972256940 103 VEDLMKSSFRIvNNPIAEKGCSCGSSFA 130
Cdd:COG0316    80 VEELLGSGFKF-NNPNAKSSCGCGESFS 106
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 27-130 3.13e-38

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 124.88  E-value: 3.13e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  27 IKVTNKAASRLKEVVDNGER----LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGATVDF 102
Cdd:COG0316     2 ITLTDAAAKRIKRLLAKEGNpglgLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQ--DGVKVVVDPKSLPYLDGTEIDY 79

                          90       100
                  ....*....|....*....|....*...
gi 1972256940 103 VEDLMKSSFRIvNNPIAEKGCSCGSSFA 130
Cdd:COG0316    80 VEELLGSGFKF-NNPNAKSSCGCGESFS 106
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 28-130 5.19e-33

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 111.52  E-value: 5.19e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  28 KVTNKAASRLKEVVDNGER----LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKssENGAEIVVDELSLGFLKGATVDFV 103
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEpnlgLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFE--QDGVKVVVDPKSLPYLDGSEIDYV 78

                          90       100
                  ....*....|....*....|....*..
gi 1972256940 104 EDLMKSSFRIVnNPIAEKGCSCGSSFA 130
Cdd:TIGR00049  79 EELLGSGFTFT-NPNAKGTCGCGKSFS 104
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 25-132 5.96e-31

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 108.48  E-value: 5.96e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  25 QEIKVTNKAASRLKEVVDN-----GERLRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGAT 99
Cdd:PLN03082   53 DAVHMTDNCIRRLKELQTSepsaeDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEK--DGVKLVVDNISYDFVKGAT 130

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1972256940 100 VDFVEDLMKSSFRIVNNPIAEKGCSCGSSFAPK 132
Cdd:PLN03082  131 VDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 163
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 26-126 6.66e-14

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 63.05  E-value: 6.66e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  26 EIKVTNKAASRLKEVVDNGER-LRLEVDGGG-------CS-GFEYKIRLDKKINND-DLLWKSseNGAEIVVDELSLGFL 95
Cdd:pfam01521   2 KITITDAAAERLKKLLAGDKKeLRLDVDDGGgpyskggCSiGGKFSLVLVDEPDPDyDEVIES--NGGPIYVDSYSLPFL 79

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1972256940  96 -KGATVDFVEDLMKSSFRIvNNPIAEKGCSCG 126
Cdd:pfam01521  80 dEGLTLDFVEDLGTLGLKS-DNGNLDGNVGCG 110
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 27-130 3.13e-38

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 124.88  E-value: 3.13e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  27 IKVTNKAASRLKEVVDNGER----LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGATVDF 102
Cdd:COG0316     2 ITLTDAAAKRIKRLLAKEGNpglgLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQ--DGVKVVVDPKSLPYLDGTEIDY 79

                          90       100
                  ....*....|....*....|....*...
gi 1972256940 103 VEDLMKSSFRIvNNPIAEKGCSCGSSFA 130
Cdd:COG0316    80 VEELLGSGFKF-NNPNAKSSCGCGESFS 106
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 28-130 5.19e-33

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 111.52  E-value: 5.19e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  28 KVTNKAASRLKEVVDNGER----LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKssENGAEIVVDELSLGFLKGATVDFV 103
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEpnlgLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFE--QDGVKVVVDPKSLPYLDGSEIDYV 78

                          90       100
                  ....*....|....*....|....*..
gi 1972256940 104 EDLMKSSFRIVnNPIAEKGCSCGSSFA 130
Cdd:TIGR00049  79 EELLGSGFTFT-NPNAKGTCGCGKSFS 104
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 25-132 5.96e-31

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 108.48  E-value: 5.96e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  25 QEIKVTNKAASRLKEVVDN-----GERLRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGAT 99
Cdd:PLN03082   53 DAVHMTDNCIRRLKELQTSepsaeDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEK--DGVKLVVDNISYDFVKGAT 130

                          90       100       110
                  ....*....|....*....|....*....|...
gi 1972256940 100 VDFVEDLMKSSFRIVNNPIAEKGCSCGSSFAPK 132
Cdd:PLN03082  131 VDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 163
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 27-130 4.81e-30

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 104.62  E-value: 4.81e-30
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  27 IKVTNKAASRLKEVVDNGE----RLRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGATVDF 102
Cdd:PRK13623   10 LVFTDAAAAKVKELIEEEGnpdlKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEK--QGVTLVVDPMSLQYLVGAEVDY 87

                          90       100
                  ....*....|....*....|....*...
gi 1972256940 103 VEDLMKSSFrIVNNPIAEKGCSCGSSFA 130
Cdd:PRK13623   88 TEGLEGSRF-VIKNPNAKTTCGCGSSFS 114
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 25-129 4.25e-18

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 73.71  E-value: 4.25e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  25 QEIKVTNKAASRLKEVVDNGER---LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKssENGAEIVVDELSLGFLKGATVD 101
Cdd:TIGR01997   1 AVITLTDAAAIHIRELVAKRPEavgIRLGVKKTGCAGMEYVLDLVSEPKKDDDLIE--HDGAKVFVAPEAVLFILGTQVD 78

                          90       100
                  ....*....|....*....|....*...
gi 1972256940 102 FVEDLMKSSFRIvNNPIAEKGCSCGSSF 129
Cdd:TIGR01997  79 FVRTTLRQGFKF-NNPNATSACGCGESF 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 25-129 4.03e-14

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 63.99  E-value: 4.03e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  25 QEIKVTNKAASRLKEVVDNGER---LRLEVDGGGCSGFEYKIRLDKKINNDDLLWKSseNGAEIVVDELSLGFLKGATVD 101
Cdd:PRK09504   16 QGLTLTPAAAAHIRELMAKQPGmkgVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEH--DGAKLFVPLQAMPFIDGTEVD 93

                          90       100
                  ....*....|....*....|....*...
gi 1972256940 102 FVEDLMKSSFRIvNNPIAEKGCSCGSSF 129
Cdd:PRK09504   94 YVREGLNQIFKF-HNPKAQNECGCGESF 120
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 27-129 6.06e-14

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 62.92  E-value: 6.06e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  27 IKVTNKAASRLKEVVDN---GERLRLEVDGGGCSGFEYKIRLDKKINNDDLLWKssENGAEIVVDELSLGFLKGATVDFV 103
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANrgkGFGLRLGVKTSGCSGMAYVLEFVDEPTPDDIVFE--DKGVKIVIDGKSLQYLDGTQLDFV 78

                          90       100
                  ....*....|....*....|....*.
gi 1972256940 104 EDLMKSSFRIvNNPIAEKGCSCGSSF 129
Cdd:TIGR02011  79 KEGLNEGFKF-TNPNVKDECGCGESF 103
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 26-126 6.66e-14

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 63.05  E-value: 6.66e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  26 EIKVTNKAASRLKEVVDNGER-LRLEVDGGG-------CS-GFEYKIRLDKKINND-DLLWKSseNGAEIVVDELSLGFL 95
Cdd:pfam01521   2 KITITDAAAERLKKLLAGDKKeLRLDVDDGGgpyskggCSiGGKFSLVLVDEPDPDyDEVIES--NGGPIYVDSYSLPFL 79

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1972256940  96 -KGATVDFVEDLMKSSFRIvNNPIAEKGCSCG 126
Cdd:pfam01521  80 dEGLTLDFVEDLGTLGLKS-DNGNLDGNVGCG 110
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
27-129 9.25e-14

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 62.58  E-value: 9.25e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1972256940  27 IKVTNKAASRLKEVVDN---GERLRLEVDGGGCSGFEYKIRLDKKINNDDLLWKssENGAEIVVDELSLGFLKGATVDFV 103
Cdd:PRK09502    3 ITLSDSAAARVNTFLANrgkGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFE--DKGVKVVVDGKSLQFLDGTQLDFV 80

                          90       100
                  ....*....|....*....|....*.
gi 1972256940 104 EDLMKSSFRIvNNPIAEKGCSCGSSF 129
Cdd:PRK09502   81 KEGLNEGFKF-TNPNVKDECGCGESF 105
PRK11190 PRK11190
iron-sulfur cluster biogenesis protein NfuA;
81-109 8.52e-04

iron-sulfur cluster biogenesis protein NfuA;


Pssm-ID: 183027 [Multi-domain]  Cd Length: 192  Bit Score: 37.30  E-value: 8.52e-04
                          10        20
                  ....*....|....*....|....*....
gi 1972256940  81 NGAEIVVDELSLGFLKGATVDFVEDLMKS 109
Cdd:PRK11190   59 DGFSAYVDELSAPFLEDAEIDFVTDQLGS 87
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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