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Conserved domains on  [gi|28178821|ref|NP_008830|]
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isocitrate dehydrogenase [NAD] subunit beta, mitochondrial isoform a precursor [Homo sapiens]

Protein Classification

isocitrate/isopropylmalate dehydrogenase family protein( domain architecture ID 296)

isocitrate/isopropylmalate dehydrogenase family protein such as isocitrate dehydrogenase that in the Krebs cycle catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2, and isopropylmalate dehydrogenase that in leucine biosynthesis catalyzes the oxidation and decarboxylation of 3-isopropyl-l-malate to 4-methyl-2-oxovalerate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Iso_dh super family cl00445
Isocitrate/isopropylmalate dehydrogenase;
47-380 1.13e-174

Isocitrate/isopropylmalate dehydrogenase;


The actual alignment was detected with superfamily member TIGR00175:

Pssm-ID: 444908  Cd Length: 333  Bit Score: 489.58  E-value: 1.13e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821    47 GSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEkLEQVLSSMKENKVAIIGKIHTPMEyKGELA 126
Cdd:TIGR00175   2 GKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDGKTEI-PDEAVESIKRNKVALKGPLETPIG-KGGHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   127 SYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYAT 206
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   207 KKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVG 286
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   287 GAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGY 366
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 28178821   367 STTTDFIKSVIGHL 380
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 47-380 1.13e-174

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 489.58  E-value: 1.13e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821    47 GSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEkLEQVLSSMKENKVAIIGKIHTPMEyKGELA 126
Cdd:TIGR00175   2 GKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDGKTEI-PDEAVESIKRNKVALKGPLETPIG-KGGHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   127 SYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYAT 206
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   207 KKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVG 286
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   287 GAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGY 366
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 28178821   367 STTTDFIKSVIGHL 380
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 50-381 1.46e-126

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 368.42  E-value: 1.46e-126
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   50 PVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHhlsEVQNmASEEKLEQVLSSMKENKVAIIGKIHTPMeyKGELASYD 129
Cdd:PLN00123  32 AVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERY---EVHG-DMKKVPEEVLESIRRNKVCLKGGLATPV--GGGVSSLN 105

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  130 MRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKG 209
Cdd:PLN00123 106 VQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNN 185

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  210 RGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAG 289
Cdd:PLN00123 186 RKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTG 265

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  290 VVPGESYSAEYAVFETGARH---PFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGY 366
Cdd:PLN00123 266 VMPGGNVGADHAVFEQGASAgnvGNEKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYRTKDLGGS 345

                        330
                 ....*....|....*
gi 28178821  367 STTTDFIKSVIGHLQ 381
Cdd:PLN00123 346 STTQEVVDAVIANLD 360
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 48-380 5.27e-101

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 302.70  E-value: 5.27e-101
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  48 SFPVTMLPGDGVGPELMHAVKEVFKAAA----VPVEFQEH-----HLSEVQNMASEEkleqVLSSMKENKVAIIGKIHTP 118
Cdd:COG0473   1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEAdiggaAYDKTGTPLPDE----TLEALRKADAILLGAVGGP 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 119 -MEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRH-----NNLDLVIIREQTEGEYSSLEHESARG----VIECLKI 188
Cdd:COG0473  77 kWDDGVRPESGLLALRKELDLYANLRPAKLYPGLPSPLkpeivEGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRV 156

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 189 VTRAKSQRIAKFAFDYAtKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLV 268
Cdd:COG0473 157 YTRKGIERIARYAFELA-RKRRKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIV 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 269 MPNLYGNIIDNLAAGLVGGAGVVPGESYSAEY-AVFEtgARH---PfaQAVGRNIANPTAMLLSASNMLRHLNLEYHSSM 344
Cdd:COG0473 236 TENLFGDILSDLAAGLTGSLGLAPSANIGDEGkALFE--PVHgsaP--DIAGKGIANPIATILSAAMMLRHLGEEEAADA 311

                       330       340       350
                ....*....|....*....|....*....|....*.
gi 28178821 345 IADAvKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
Cdd:COG0473 312 IEAA-VEKVLAEGVRTPDLGGKAGTSEMGDAIIAAL 346
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
51-376 4.63e-87

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 267.24  E-value: 4.63e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821    51 VTMLPGDGVGPELMHAVKEVFKAAAVPV----EFQEHHLSEVQNMASEEKL-EQVLSSMKENKVAIIGKIHTPMEYKGEL 125
Cdd:pfam00180   2 IAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAIDETGEPLpDETLEACKKADAVLLGAVGGPKWDPAGV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   126 ASYD--MRLRRKLDLFANVVHVKSLPG------YMTRHNNLDLVIIREQTEGEYSSLEHE---SARGVIECLKIVTRAKS 194
Cdd:pfam00180  82 RPENglLALRKELGLFANLRPAKVFPPlgdaspLKNEVEGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDEI 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   195 QRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYG 274
Cdd:pfam00180 162 ERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLFG 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   275 NIIDNLAAGLVGGAGVVPGESYSA-EYAVFEtgARHPFAQAV-GRNIANPTAMLLSASNMLRH-LNLEYHSSMIADAvKK 351
Cdd:pfam00180 242 DILSDEASMLTGSLGLLPSASLGAnGFGIFE--PVHGSAPDIaGKGIANPIATILSAAMMLRYsLGLEDAADKIEAA-VL 318

                         330       340
                  ....*....|....*....|....*...
gi 28178821   352 VIKVGKVRTRDMGG---YSTTTDFIKSV 376
Cdd:pfam00180 319 KVLESGIRTGDLAGsatYVSTSEFGEAV 346
 
Name Accession Description Interval E-value
mito_nad_idh TIGR00175
isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent ...
 47-380 1.13e-174

isocitrate dehydrogenase, NAD-dependent, mitochondrial type; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the multimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates. Mitochondrial NAD-dependent isocitrate dehydrogenases (IDH) resemble prokaryotic NADP-dependent IDH and 3-isopropylmalate dehydrogenase (an NAD-dependent enzyme) more closely than they resemble eukaryotic NADP-dependent IDH. The mitochondrial NAD-dependent isocitrate dehydrogenase is believed to be an alpha(2)-beta-gamma heterotetramer. All subunits are homologous and found by this model. The NADP-dependent IDH of Thermus aquaticus thermophilus strain HB8 resembles these NAD-dependent IDH, except for the residues involved in cofactor specificity, much more closely than it resembles other prokaryotic NADP-dependent IDH, including that of Thermus aquaticus strain YT1. [Energy metabolism, TCA cycle]


Pssm-ID: 272942  Cd Length: 333  Bit Score: 489.58  E-value: 1.13e-174
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821    47 GSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEkLEQVLSSMKENKVAIIGKIHTPMEyKGELA 126
Cdd:TIGR00175   2 GKYTVTLIPGDGIGPEISGSVKKIFRAANVPIEFEEIDVSPQTDGKTEI-PDEAVESIKRNKVALKGPLETPIG-KGGHR 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   127 SYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYAT 206
Cdd:TIGR00175  80 SLNVALRKELDLYANVVHCKSLPGFKTRHEDVDIVIIRENTEGEYSGLEHESVPGVVESLKVITRDKSERIARYAFEYAR 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   207 KKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVG 286
Cdd:TIGR00175 160 KNGRKKVTAVHKANIMKLADGLFLNVCREVAKEYPDITFESMIVDNTCMQLVSRPSQFDVMVMPNLYGNILSNLGAGLVG 239

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   287 GAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGY 366
Cdd:TIGR00175 240 GPGLVPGANIGRDYAVFEPGVRHTGPDIAGQNIANPTALILSSVMMLNHLGLKEHADRIQKAVLSTIAEGKNRTKDLGGT 319

                         330
                  ....*....|....
gi 28178821   367 STTTDFIKSVIGHL 380
Cdd:TIGR00175 320 ATTSDFTEAVIKRL 333
PLN00123 PLN00123
isocitrate dehydrogenase (NAD+)
 50-381 1.46e-126

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215065  Cd Length: 360  Bit Score: 368.42  E-value: 1.46e-126
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   50 PVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHhlsEVQNmASEEKLEQVLSSMKENKVAIIGKIHTPMeyKGELASYD 129
Cdd:PLN00123  32 AVTLIPGDGIGPLVTGAVEQVMEAMHAPVYFERY---EVHG-DMKKVPEEVLESIRRNKVCLKGGLATPV--GGGVSSLN 105

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  130 MRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKG 209
Cdd:PLN00123 106 VQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNN 185

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  210 RGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAG 289
Cdd:PLN00123 186 RKKVTAVHKANIMKLADGLFLESCREVAKKYPGIKYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTG 265

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  290 VVPGESYSAEYAVFETGARH---PFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGY 366
Cdd:PLN00123 266 VMPGGNVGADHAVFEQGASAgnvGNEKLVEQKKANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGKYRTKDLGGS 345

                        330
                 ....*....|....*
gi 28178821  367 STTTDFIKSVIGHLQ 381
Cdd:PLN00123 346 STTQEVVDAVIANLD 360
PLN00118 PLN00118
isocitrate dehydrogenase (NAD+)
 47-380 2.84e-123

isocitrate dehydrogenase (NAD+)


Pssm-ID: 215062  Cd Length: 372  Bit Score: 360.73  E-value: 2.84e-123
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   47 GSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSE-----VQNMASEEKLEqvlsSMKENKVAIIGKIHTPMEy 121
Cdd:PLN00118  40 TPITATLFPGDGIGPEIAESVKQVFTAAGVPIEWEEHYVGTtvdprTGSFLTWESLE----SVRRNKVGLKGPMATPIG- 114

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  122 KGElASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFA 201
Cdd:PLN00118 115 KGH-RSLNLTLRKELGLYANVRPCYSLPGYKTRYDDVDLVTIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYA 193

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  202 FDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLA 281
Cdd:PLN00118 194 FHYAKTHGRKRVSAIHKANIMKKTDGLFLKCCREVAEKYPEIVYEEVIIDNCCMMLVKNPALFDVLVMPNLYGDIISDLC 273

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  282 AGLVGGAGVVP-GESYSAEYAVFEtgARHPFAQAV-GRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVR 359
Cdd:PLN00118 274 AGLIGGLGLTPsCNIGENGLALAE--AVHGSAPDIaGKNLANPTALLLSAVMMLRHLKLNEQAEQIHNAILNTIAEGKYR 351

                        330       340
                 ....*....|....*....|.
gi 28178821  360 TRDMGGYSTTTDFIKSVIGHL 380
Cdd:PLN00118 352 TADLGGSSTTTDFTKAICDHL 372
LeuB COG0473
Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid ...
 48-380 5.27e-101

Isocitrate/isopropylmalate dehydrogenase [Energy production and conversion, Amino acid transport and metabolism]; Isocitrate/isopropylmalate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440241  Cd Length: 346  Bit Score: 302.70  E-value: 5.27e-101
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  48 SFPVTMLPGDGVGPELMHAVKEVFKAAA----VPVEFQEH-----HLSEVQNMASEEkleqVLSSMKENKVAIIGKIHTP 118
Cdd:COG0473   1 TYKIAVLPGDGIGPEVVAAALKVLEAAAerfgLDFEFEEAdiggaAYDKTGTPLPDE----TLEALRKADAILLGAVGGP 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 119 -MEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRH-----NNLDLVIIREQTEGEYSSLEHESARG----VIECLKI 188
Cdd:COG0473  77 kWDDGVRPESGLLALRKELDLYANLRPAKLYPGLPSPLkpeivEGIDLVIVRENTEGLYFGIGGRIGTGtgeeVAIDTRV 156

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 189 VTRAKSQRIAKFAFDYAtKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLV 268
Cdd:COG0473 157 YTRKGIERIARYAFELA-RKRRKKVTSVDKANVLKLTSGLWREVVREVAKEYPDVELDHMYVDAAAMQLVRNPEQFDVIV 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 269 MPNLYGNIIDNLAAGLVGGAGVVPGESYSAEY-AVFEtgARH---PfaQAVGRNIANPTAMLLSASNMLRHLNLEYHSSM 344
Cdd:COG0473 236 TENLFGDILSDLAAGLTGSLGLAPSANIGDEGkALFE--PVHgsaP--DIAGKGIANPIATILSAAMMLRHLGEEEAADA 311

                       330       340       350
                ....*....|....*....|....*....|....*.
gi 28178821 345 IADAvKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
Cdd:COG0473 312 IEAA-VEKVLAEGVRTPDLGGKAGTSEMGDAIIAAL 346
PRK08997 PRK08997
isocitrate dehydrogenase; Provisional
 51-380 4.72e-100

isocitrate dehydrogenase; Provisional


Pssm-ID: 181606  Cd Length: 334  Bit Score: 300.10  E-value: 4.72e-100
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFQehhLSEVQNMASEEKLE----QVLSSMKENKVAIIGKIHTPMeykGE-L 125
Cdd:PRK08997   5 ITVIPGDGIGPSIIDATLKILDKLGCDFEYE---FADAGLTALEKHGEllpqRTLDLIEKNKIALKGPLTTPV---GEgF 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  126 ASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHE-SARG-VIECLKIVTRAKSQRIAKFAFD 203
Cdd:PRK08997  79 TSINVTLRKKFDLYANVRPVLSFPGTKARYDNIDIITVRENTEGMYSGEGQTvSEDGeTAEATSIITRKGAERIVRFAYE 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  204 YATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAG 283
Cdd:PRK08997 159 LARKEGRKKVTAVHKANIMKSTSGLFLKVAREVALRYPDIEFEEMIVDATCMQLVMNPEQFDVIVTTNLFGDILSDLCAG 238

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  284 LVGGAGVVPGESYSAEYAVFEtgARHPFAQAV-GRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRD 362
Cdd:PRK08997 239 LVGGLGMAPGANIGRDAAIFE--AVHGSAPDIaGKNLANPTSVILAAIQMLEYLGMPDKAERIRKAIVAVIEAGDRTTRD 316

                        330
                 ....*....|....*...
gi 28178821  363 MGGYSTTTDFIKSVIGHL 380
Cdd:PRK08997 317 LGGTHGTTDFTQAVIDRL 334
Iso_dh pfam00180
Isocitrate/isopropylmalate dehydrogenase;
51-376 4.63e-87

Isocitrate/isopropylmalate dehydrogenase;


Pssm-ID: 425507  Cd Length: 346  Bit Score: 267.24  E-value: 4.63e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821    51 VTMLPGDGVGPELMHAVKEVFKAAAVPV----EFQEHHLSEVQNMASEEKL-EQVLSSMKENKVAIIGKIHTPMEYKGEL 125
Cdd:pfam00180   2 IAVLPGDGIGPEVMAAALKVLKAALEKAplefEFEERDVGGAAIDETGEPLpDETLEACKKADAVLLGAVGGPKWDPAGV 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   126 ASYD--MRLRRKLDLFANVVHVKSLPG------YMTRHNNLDLVIIREQTEGEYSSLEHE---SARGVIECLKIVTRAKS 194
Cdd:pfam00180  82 RPENglLALRKELGLFANLRPAKVFPPlgdaspLKNEVEGVDIVIVRELTGGIYFGIEKGikgSGNEVAVDTKLYSRDEI 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   195 QRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYG 274
Cdd:pfam00180 162 ERIARVAFELARKRGRKKVTSVDKANVLKSSDLWRKIVTEVAKAEYPDVELEHQLVDNAAMQLVKNPSQFDVIVTPNLFG 241

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   275 NIIDNLAAGLVGGAGVVPGESYSA-EYAVFEtgARHPFAQAV-GRNIANPTAMLLSASNMLRH-LNLEYHSSMIADAvKK 351
Cdd:pfam00180 242 DILSDEASMLTGSLGLLPSASLGAnGFGIFE--PVHGSAPDIaGKGIANPIATILSAAMMLRYsLGLEDAADKIEAA-VL 318

                         330       340
                  ....*....|....*....|....*...
gi 28178821   352 VIKVGKVRTRDMGG---YSTTTDFIKSV 376
Cdd:pfam00180 319 KVLESGIRTGDLAGsatYVSTSEFGEAV 346
PRK09222 PRK09222
NADP-dependent isocitrate dehydrogenase;
47-348 5.23e-86

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 236416 [Multi-domain]  Cd Length: 482  Bit Score: 269.07  E-value: 5.23e-86
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   47 GSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVE----------FQEHHLSEVqnmaSEEKLEqvlsSMKENKVAIIGKIH 116
Cdd:PRK09222   3 EKTPITVAYGDGIGPEIMEAVLKILEAAGAPLEietieigekvYKKGWTSGI----SPSAWE----SIRRTKVLLKAPIT 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  117 TPME--YKgelaSYDMRLRRKLDLFANVVHVKSL-PGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAK 193
Cdd:PRK09222  75 TPQGggYK----SLNVTLRKTLGLYANVRPCVSYhPFVETKHPNLDVVIIRENEEDLYAGIEHRQTPDVYQCLKLISRPG 150

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  194 SQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLY 273
Cdd:PRK09222 151 SEKIIRYAFEYARANGRKKVTCLTKDNIMKLTDGLFHKVFDEIAKEYPDIEAEHYIVDIGAARLATNPENFDVIVTPNLY 230

                        250       260       270       280       290       300       310
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 28178821  274 GNIIDNLAAGLVGGAGVVPGESYSAEYAVFEtgARHPFAQAV-GRNIANPTAMLLSASNMLRHLNLEYHSSMIADA 348
Cdd:PRK09222 231 GDILSDIAAEISGSVGLAGSANIGEEYAMFE--AVHGSAPDIaGKNIANPSGLLNAAVMMLVHIGQFDIAELIENA 304
PRK14025 PRK14025
multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional
 51-380 4.49e-78

multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase; Provisional


Pssm-ID: 184462  Cd Length: 330  Bit Score: 243.89  E-value: 4.49e-78
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFqehhlseVQNMASEEKLEQVLSSMKENKVAIIGKIH-TPMEYKGELA-SY 128
Cdd:PRK14025   4 ICVIEGDGIGKEVVPAALHVLEATGLPFEF-------VYAEAGDEVFEKTGKALPEETIEAAKEADaVLFGAAGETAaDV 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  129 DMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKK 208
Cdd:PRK14025  77 IVKLRRILDTYANVRPVKSYKGVKCLYPDIDYVIVRENTEGLYKGIEAEIADGVTVATRVITRKASERIFRFAFEMAKRR 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  209 ----GRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGL 284
Cdd:PRK14025 157 kkmgKEGKVTCAHKANVLKKTDGLFKKTFYEVAKEYPDIKAEDYYVDAMNMYIITRPQTFDVVVTSNLFGDILSDGAAGL 236

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  285 VGGAGVVPGESYSAEYAVFEtgARHPFA-QAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVrTRDM 363
Cdd:PRK14025 237 VGGLGLAPSANIGDKYGLFE--PVHGSApDIAGKGIANPTATILTAVLMLRHLGENEEADKVEKALEEVLALGLT-TPDL 313

                        330
                 ....*....|....*..
gi 28178821  364 GGYSTTTDFIKSVIGHL 380
Cdd:PRK14025 314 GGNLSTMEMAEEVAKRV 330
PRK00772 PRK00772
3-isopropylmalate dehydrogenase; Provisional
 51-383 2.99e-53

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 234832  Cd Length: 358  Bit Score: 180.30  E-value: 2.99e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   51 VTMLPGDGVGPELMHAVKEVFKAAA----VPVEFQEHHLS-----EVQNMASEEKLEQVLSSMkenkvAII-GKIHTPme 120
Cdd:PRK00772   5 IAVLPGDGIGPEVMAEAVKVLDAVAekfgFDFEFEEALVGgaaidAHGVPLPEETLEACRAAD-----AVLlGAVGGP-- 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  121 yKGELASYDMR-------LRRKLDLFANVVHVKSLPGYMT-------RHNNLDLVIIREQTEGEYS------SLEHESAR 180
Cdd:PRK00772  78 -KWDNLPPDVRpergllaLRKELGLFANLRPAKLYPGLADasplkpeIVAGLDILIVRELTGGIYFgeprgrEGLGGEER 156

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  181 GVIEClkIVTRAKSQRIAKFAFDYAtKKGRGKVTAVHKANIMKlGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 260
Cdd:PRK00772 157 AFDTM--VYTREEIERIARVAFELA-RKRRKKVTSVDKANVLE-SSRLWREVVTEVAKEYPDVELSHMYVDNAAMQLVRN 232

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE-YAVFETGarHPFA-QAVGRNIANPTAMLLSASNMLRH-LN 337
Cdd:PRK00772 233 PKQFDVIVTENLFGDILSDEAAMLTGSLGMLPSASLGESgPGLYEPI--HGSApDIAGKGIANPIATILSAAMMLRYsLG 310

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|....*....
gi 28178821  338 LEYHSSMIADAvKKVIKVGKVRTRDM---GGYSTTTDFIKSVIGHLQTK 383
Cdd:PRK00772 311 LEEAADAIEAA-VEKVLAQGYRTADIaegGGKVSTSEMGDAILAALAEG 358
leuB TIGR00169
3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including ...
 51-380 7.40e-51

3-isopropylmalate dehydrogenase; Several NAD- or NADP-dependent dehydrogenases, including 3-isopropylmalate dehydrogenase, tartrate dehydrogenase, and the dimeric forms of isocitrate dehydrogenase, share a nucleotide binding domain unrelated to that of lactate dehydrogenase and its homologs. These enzymes dehydrogenate their substates at a H-C-OH site adjacent to a H-C-COOH site; the latter carbon, now adjacent to a carbonyl group, readily decarboxylates.Among these decarboxylating dehydrogenases of hydroxyacids, overall sequence homology indicates evolutionary history rather than actual substrate or cofactor specifity, which may be toggled experimentally by replacement of just a few amino acids. 3-isopropylmalate dehydrogenase is an NAD-dependent enzyme and should have a sequence resembling HGSAPDI around residue 340. The subtrate binding loop should include a sequence resembling E[KQR]X(0,1)LLXXR around residue 115. Other contacts of importance are known from crystallography but not detailed here.This model will not find all isopropylmalate dehydrogenases; the enzyme from Sulfolobus sp. strain 7 is more similar to mitochondrial NAD-dependent isocitrate dehydrogenases than to other known isopropylmalate dehydrogenases and was omitted to improve the specificity of the model. It scores below the cutoff and below some enzymes known not to be isopropylmalate dehydrogenase. [Amino acid biosynthesis, Pyruvate family]


Pssm-ID: 272939  Cd Length: 346  Bit Score: 173.74  E-value: 7.40e-51
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821    51 VTMLPGDGVGPELMHAVKEVFKAAA----VPVEFQEHHLSEVQNMASEEKL-EQVLSSMKENKVAIIGKI------HTPM 119
Cdd:TIGR00169   2 IAVLPGDGIGPEVMAQALKVLKAVAerfgLKFEFEEHLIGGAAIDATGQPLpEETLKGCKEADAVLLGAVggpkwdNLPR 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   120 EYKGELASydMRLRRKLDLFANVVHVKSLPGYMTRH-------NNLDLVIIREQTEGEY--SSLEHESARGVIECLKiVT 190
Cdd:TIGR00169  82 DQRPEQGL--LKLRKSLDLFANLRPAKVFPGLEDLSplkeeiaKGVDFVVVRELTGGIYfgEPKGREGEGEAWDTEV-YT 158

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   191 RAKSQRIAKFAFDYATKKgRGKVTAVHKANIMKLGDgLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMP 270
Cdd:TIGR00169 159 VPEIERIARVAFEMARKR-RKKVTSVDKANVLESSR-LWRKTVEEIAKEYPDVELEHQYIDNAAMQLVKSPTQFDVVVTS 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   271 NLYGNIIDNLAAGLVGGAGVVPGESYSAE-YAVFE-TGARHPfaQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADA 348
Cdd:TIGR00169 237 NLFGDILSDEASVITGSLGMLPSASLGSDgFGLFEpVHGSAP--DIAGKGIANPIAQILSAAMLLRYSFNLEEAADAIEA 314

                         330       340       350
                  ....*....|....*....|....*....|..
gi 28178821   349 VKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHL 380
Cdd:TIGR00169 315 AVKKVLAEGYRTPDLGSSATTAVGTAEMGEEL 346
PRK03437 PRK03437
3-isopropylmalate dehydrogenase; Provisional
 53-369 7.46e-43

3-isopropylmalate dehydrogenase; Provisional


Pssm-ID: 179579  Cd Length: 344  Bit Score: 152.39  E-value: 7.46e-43
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   53 MLPGDGVGPELMH-AVKeVFKAAA---VPVEFQEHHLSEVQNMASEEKL-EQVLSSMKENKVAIIGKIHTP------MEy 121
Cdd:PRK03437   9 VIPGDGIGPEVVAeALK-VLDAVAaggPGVETTEYDLGARRYLRTGETLpDSVLAELRQHDAILLGAIGDPsvpsgvLE- 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  122 KGELasydMRLRRKLDLFANVVHVKSLPGYMTRHNN---LDLVIIREQTEGEYSS--------LEHESARGVieclKIVT 190
Cdd:PRK03437  87 RGLL----LKLRFALDHYVNLRPSKLYPGVTSPLAGpgdIDFVVVREGTEGPYTGnggalrvgTPHEVATEV----SVNT 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  191 RAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMP 270
Cdd:PRK03437 159 AFGVERVVRDAFERAQKRPRKHLTLVHKTNVLTFAGDLWQRTVDEVAAEYPDVTVDYQHVDAATIFMVTDPSRFDVIVTD 238

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  271 NLYGNIIDNLAAGLVGGAGVVPGESYSAEYA---VFE-TGARHPfaQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIA 346
Cdd:PRK03437 239 NLFGDIITDLAAAVTGGIGLAASGNINPTGTnpsMFEpVHGSAP--DIAGQGIADPTAAILSVALLLDHLGEEDAAARIE 316

                        330       340
                 ....*....|....*....|...
gi 28178821  347 DAvkkviKVGKVRTRDMGGYSTT 369
Cdd:PRK03437 317 AA-----VEADLAERGKMGRSTA 334
PRK08194 PRK08194
tartrate dehydrogenase; Provisional
 49-382 5.01e-41

tartrate dehydrogenase; Provisional


Pssm-ID: 181281  Cd Length: 352  Bit Score: 147.95  E-value: 5.01e-41
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   49 FPVTMLPGDGVGPELMHAVKEVFKAAA-----VPVEFQEHHLS-----EVQNMASEEKLEQvlssMKENKVAIIGKIHTP 118
Cdd:PRK08194   4 FKIAVIPGDGVGKEVVPAAVRVLKAVAevhggLKFEFTEFPWSceyylEHGEMMPEDGLEQ----LKQFDAIFLGAVGNP 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  119 MEYKGELASYDM--RLRRKLDLFANVVHVKSLPGY---MTRHNNLDLVIIREQTEGEYSSLEHESARGVIECL---KIVT 190
Cdd:PRK08194  80 KLVPDHISLWGLliKIRREFEQVINIRPAKQLRGIkspLANPKDFDLLVVRENSEGEYSEVGGRIHRGEDEIAiqnAVFT 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  191 RAKSQRIAKFAFDYATKKgRGKVTAVHKAN----IMKLGDGLFlqccEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDV 266
Cdd:PRK08194 160 RKGTERAMRYAFELAAKR-RKHVTSATKSNgivhSMPFWDEVF----QEVGKDYPEIETDSQHIDALAAFFVTRPEEFDV 234

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  267 LVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAE--Y-AVFEtgARHPFA-QAVGRNIANPTAMLLSASNMLRHLNLEYHS 342
Cdd:PRK08194 235 IVASNLFGDILTDIGAAIMGSIGIAPAANINVNgkYpSMFE--PVHGSApDIAGKGIANPIGQIWTAKLMLDHFGEEELG 312

                        330       340       350       360
                 ....*....|....*....|....*....|....*....|
gi 28178821  343 SMIADAvKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQT 382
Cdd:PRK08194 313 SHLLDV-IEDVTEDGIKTPDIGGRATTDEVTDEIISRLKK 351
PRK06451 PRK06451
NADP-dependent isocitrate dehydrogenase;
56-348 1.16e-37

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 235803  Cd Length: 412  Bit Score: 140.35  E-value: 1.16e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   56 GDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASE--EKL------EQVLSSMKENKVAIIGKIHTPMEyKGeLAS 127
Cdd:PRK06451  31 GDGIGPEITHAAMKVINKAVEKAYGSDREIKWVEVLAGDkaEKLtgnrfpKESEELIEKYRVLLKGPLETPIG-KG-WKS 108

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  128 YDMRLRRKLDLFANVVHVKSLPGY---MTRHNNLDLVIIREQTEGEYSSLEH----ESARGVIECL-------------- 186
Cdd:PRK06451 109 INVAIRLMLDLYANIRPVKYIPGIespLKNPEKIDLIIFRENTDDLYRGIEYpydsEEAKKIRDFLrkelgveveddtgi 188

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  187 --KIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCC---------------EEVAELYP------KI 243
Cdd:PRK06451 189 giKLISKFKTQRIARMAIKYAIDHKRKKVTIMHKGNVMKYTEGAFREWAyevalkefrdyvvteEEVTKNYNgvppsgKV 268

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  244 KFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFEtgARHPFA-QAVGRNIANP 322
Cdd:PRK06451 269 IINDRIADNMFQQIIIRPDEYDIILAPNVNGDYISDAAGALVGNIGMLGGANIGDTGGMFE--AIHGTApKYAGKNVANP 346

                        330       340
                 ....*....|....*....|....*.
gi 28178821  323 TAMLLSASNMLRHLNLEYHSSMIADA 348
Cdd:PRK06451 347 TGIIKGGELMLRFMGWDKAADLIDKA 372
Icd COG0538
Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part ...
 56-336 8.15e-35

Isocitrate dehydrogenase [Energy production and conversion]; Isocitrate dehydrogenase is part of the Pathway/BioSystem: TCA cycle


Pssm-ID: 440304  Cd Length: 409  Bit Score: 132.54  E-value: 8.15e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  56 GDGVGPELMHAVKEVFKAA-------AVPVEFQEHHLSEvqnMASEEKLEQV----LSSMKENKVAIIGKIHTPMEykGE 124
Cdd:COG0538  26 GDGIGPEITRAIWKVIDAAvekayggKRDIEWKEVDAGE---KARDETGDWLpdetAEAIKEYGVGIKGPLTTPVG--GG 100

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 125 LASYDMRLRRKLDLFAN---VVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHES----ARGVIECL----------- 186
Cdd:COG0538 101 WRSLNVTIRQILDLYVCrrpVRYFKGVPSPVKHPEKVDIVIFRENTEDIYAGIEWKAgspeALKLIFFLedemgvtvirf 180

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 187 --------KIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAE--------------LYP--- 241
Cdd:COG0538 181 pedsgigiKPVSDEGTERLVRAAIQYALDNKRKSVTLVHKGNIMKFTEGAFKDWGYEVAEeefgdkfitegpweKYKgpk 260

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821 242 ---KIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGE--SYSAeYAVFE----TGARHpfa 312
Cdd:COG0538 261 pagKIVYKDRIADDMLQQILLRPGEYDVIATKNLNGDYISDALAAQVGGLGIAPGAniGDDG-GAEFEathgTAPKY--- 336

                       330       340
                ....*....|....*....|....
gi 28178821 313 qaVGRNIANPTAMLLSASNMLRHL 336
Cdd:COG0538 337 --AGKDSTNPGSLILSGTMMLRHR 358
PRK07006 PRK07006
isocitrate dehydrogenase; Reviewed
 56-337 2.87e-34

isocitrate dehydrogenase; Reviewed


Pssm-ID: 180792  Cd Length: 409  Bit Score: 130.95  E-value: 2.87e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   56 GDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL----------EQVLSSMKENKVAIIGKIHTPMeyKGEL 125
Cdd:PRK07006  27 GDGIGPDITPAMLKVVDAAVEKAYKGERKISWMEIYAGEKATkvygedvwlpEETLDLIREYRVAIKGPLTTPV--GGGI 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  126 ASYDMRLRRKLDLFA---NVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHES----ARGVIECL------------ 186
Cdd:PRK07006 105 RSLNVALRQELDLYVclrPVRYFKGVPSPVKRPEDTDMVIFRENSEDIYAGIEWKAgsaeAKKVIKFLqeemgvkkirfp 184

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  187 -------KIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAEL-----------YPKIK---- 244
Cdd:PRK07006 185 etsgigiKPVSEEGTERLVRAAIEYAIDNDRKSVTLVHKGNIMKFTEGAFKDWGYQLAEEefgdelidggpWDKIKnpet 264

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  245 -----FETMIIDNCCMQLVQNPYQFDVLVMPNLYGNII-DNLAAGlVGGAGVVPGESYSAEYAVFEtgARHPFA-QAVGR 317
Cdd:PRK07006 265 gkeiiVKDSIADAFLQQILLRPAEYDVIATMNLNGDYIsDALAAQ-VGGIGIAPGANINDGHAIFE--ATHGTApKYAGL 341

                        330       340
                 ....*....|....*....|
gi 28178821  318 NIANPTAMLLSASNMLRHLN 337
Cdd:PRK07006 342 DKVNPGSVILSAEMMLRHMG 361
PLN02329 PLN02329
3-isopropylmalate dehydrogenase
 45-348 2.24e-28

3-isopropylmalate dehydrogenase


Pssm-ID: 215188  Cd Length: 409  Bit Score: 114.78  E-value: 2.24e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   45 VEGSFPVTMLPGDGVGPELMHAVKEVFKAAA----VPVEFQEHHLSEVQ-NMASEEKLEQVLSSMKENKVAIIGKIHTpm 119
Cdd:PLN02329  43 GKKRYNIALLPGDGIGPEVISVAKNVLQKAGslegLEFDFQEMPVGGAAlDLVGVPLPEETFTAAKQSDAILLGAIGG-- 120

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  120 eYKGELASYDMR-------LRRKLDLFANVVHVKSLPGYMTRHN-------NLDLVIIREQTEGEYSSLE-----HESAR 180
Cdd:PLN02329 121 -YKWDKNEKHLRpemalfyLRRDLKVFANLRPATVLPQLVDASTlkkevaeGVDMMIVRELTGGIYFGEPrgitiNENGE 199

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  181 GVIECLKIVTRAKSQRIAKFAFDYATKKgRGKVTAVHKANIMKlGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQN 260
Cdd:PLN02329 200 EVGVSTEIYAAHEIDRIARVAFETARKR-RGKLCSVDKANVLD-ASILWRKRVTALASEYPDVELSHMYVDNAAMQLIRD 277

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  261 PYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRH-LNLE 339
Cdd:PLN02329 278 PKQFDTIVTNNIFGDILSDEASMITGSIGMLPSASLGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLKYgLGEE 357


                 ....*....
gi 28178821  340 YHSSMIADA 348
Cdd:PLN02329 358 KAAKRIEDA 366
PRK07362 PRK07362
NADP-dependent isocitrate dehydrogenase;
56-336 2.23e-17

NADP-dependent isocitrate dehydrogenase;


Pssm-ID: 180944  Cd Length: 474  Bit Score: 83.22  E-value: 2.23e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821   56 GDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKL----------EQVLSSMKENKVAIIGKIHTPMeyKGEL 125
Cdd:PRK07362  36 GDGTGVDIWPATQKVLDAAVAKAYGGERKINWFKVYAGDEACdlygtyqylpEDTLEAIREYGVAIKGPLTTPI--GGGI 113

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  126 ASYDMRLRRKLDLFANVVHVKSLPGYMTRHNN---LDLVIIREQTEGEYSSLEHES----ARGVIECL------------ 186
Cdd:PRK07362 114 RSLNVALRQIFDLYSCVRPCRYYAGTPSPHKNpekLDVIVYRENTEDIYMGIEWEAgdeiGDKLIKHLneevipaspelg 193

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  187 ------------KIVTRAKSQRIAKFAFDYATK--KGRGKVTAVHKANIMKLGDGLF----------------------- 229
Cdd:PRK07362 194 krqiplgsgigiKPVSKTGSQRHIRRAIEHALRlpGDKRHVTLVHKGNIMKYTEGAFrdwgyelattefrdecvteresw 273

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28178821  230 --------------------------------LQCCEEVAELYPKI-------KFETMII------DNCCMQLVQNPYQF 264
Cdd:PRK07362 274 ilsnkeknpnisiednarmiepgydsltpekkAAICAEVKEVLDSIwsshgngKWKEKVLvddriaDSIFQQIQTRPQEY 353

                        330       340       350       360       370       380       390
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 28178821  265 DVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFEtgARHPFA-QAVGRNIANPTAMLLSASNMLRHL 336
Cdd:PRK07362 354 SILATLNLNGDYISDAAAAIVGGLGMAPGANIGDNAAIFE--ATHGTApKHAGLDRINPGSVILSGVMMLEYL 424
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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