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Conserved domains on  [gi|10383750|ref|NP_009863|]
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glutathione exchanger [Saccharomyces cerevisiae S288C]

Protein Classification

ARN family MFS transporter( domain architecture ID 13023295)

ARN family MFS transporter similar to yeast siderophore iron transporter and glutathione exchanger

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
MFS_ARN_like cd17322
Yeast ARN family of Siderophore iron transporters and similar proteins of the Major ...
59-571 0e+00

Yeast ARN family of Siderophore iron transporters and similar proteins of the Major Facilitator Superfamily; The ARN family of siderophore iron transporters includes ARN1 (or ferrichrome permease), ARN2 (or triacetylfusarinine C transporter 1 or TAF1), ARN3 (or siderophore iron transporter 1 or SIT1 or ferrioxamine B permease) and ARN4 (or Enterobactin permease or ENB1). They specifically recognize siderophore-iron chelates are expressed under conditions of iron deprivation. They facilitate the uptake of both hydroxamate- and catecholate-type siderophores. This group also includes glutathione exchanger 1 (Gex1p) and Gex2p, which are proton/glutathione antiporters that import glutathione from the vacuole and exports it through the plasma membrane. The ARN family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


:

Pssm-ID: 340880 [Multi-domain]  Cd Length: 514  Bit Score: 684.42  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  59 KILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTVQVINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMG 138
Cdd:cd17322   1 LIILFFSLFLCGFAIGLDGQIRYSYQAYATSSFSKHSLLSTINVVNNIVSAVSQPPYAKLSDVFGRLELFLVSLLLYVVG 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 139 TIIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIA 218
Cdd:cd17322  81 TIIMAASTNVQTYAAGSVFYNAGYTGLNLLLTLILADFSSLKNRGFASAIPSLPYIITTWIGGNIAQAALPNSNWRWGYG 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 219 MWAFIYPLSALPIIFLILYMKYKSSKTAEWRSLKEQARKERTGGLFENLVFLFWKLDIVGILLITVSLGCILVPLTLANE 298
Cdd:cd17322 161 MWAIILPLSSLPLVLCLLYMQRKARKDGEIKELKNQETEEQTGGLSQNLVKLFWKLDIFGVILLTAGLGLILVPLTLAGG 240
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 299 TSQKWHNSKIIATLVSGGCLFFIFLYWEAKFAKSPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVLLVSMKESS 378
Cdd:cd17322 241 AKSKWKNSSIIAMIVLGGVLLVAFLLWEAKFAKYPFLPFRLLKDRTILAACLIDFFYFFSYYIFSDYLYTVLQVAVGESI 320
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 379 TSAARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKYRGGSGSHEGVIAASVIMGLSGLLCSNSV 458
Cdd:cd17322 321 TSATRITNLYSFVSTVAGIFVGFLIRKTRRYKVLVIIGVPLYIVGMGLLIRYRGGSGSHGGIIGALILIGIGGGLFTYPV 400
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 459 IVILQAMTTHSRMAVITGIQYTFSKLGAAIGASVSGAIWTQTMPNQLYKNLGNDTLAEIAYASPYTF-ISDYPWGSPERD 537
Cdd:cd17322 401 QVSVQASVSHQEMAVVTALLLTVYSIGGAVGAAISGAIWTQTLPKKLLKNLPDSLKATAAYIFPSTFvILSYPWGTPERD 480
                       490       500       510
                ....*....|....*....|....*....|....
gi 10383750 538 AVVESYRYVQRIIMTVGLACTVPFFAFTMFMRDP 571
Cdd:cd17322 481 AIIESYRYVQRILLIVALVFSAPMFALMFFLRNP 514
 
Name Accession Description Interval E-value
MFS_ARN_like cd17322
Yeast ARN family of Siderophore iron transporters and similar proteins of the Major ...
59-571 0e+00

Yeast ARN family of Siderophore iron transporters and similar proteins of the Major Facilitator Superfamily; The ARN family of siderophore iron transporters includes ARN1 (or ferrichrome permease), ARN2 (or triacetylfusarinine C transporter 1 or TAF1), ARN3 (or siderophore iron transporter 1 or SIT1 or ferrioxamine B permease) and ARN4 (or Enterobactin permease or ENB1). They specifically recognize siderophore-iron chelates are expressed under conditions of iron deprivation. They facilitate the uptake of both hydroxamate- and catecholate-type siderophores. This group also includes glutathione exchanger 1 (Gex1p) and Gex2p, which are proton/glutathione antiporters that import glutathione from the vacuole and exports it through the plasma membrane. The ARN family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340880 [Multi-domain]  Cd Length: 514  Bit Score: 684.42  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  59 KILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTVQVINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMG 138
Cdd:cd17322   1 LIILFFSLFLCGFAIGLDGQIRYSYQAYATSSFSKHSLLSTINVVNNIVSAVSQPPYAKLSDVFGRLELFLVSLLLYVVG 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 139 TIIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIA 218
Cdd:cd17322  81 TIIMAASTNVQTYAAGSVFYNAGYTGLNLLLTLILADFSSLKNRGFASAIPSLPYIITTWIGGNIAQAALPNSNWRWGYG 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 219 MWAFIYPLSALPIIFLILYMKYKSSKTAEWRSLKEQARKERTGGLFENLVFLFWKLDIVGILLITVSLGCILVPLTLANE 298
Cdd:cd17322 161 MWAIILPLSSLPLVLCLLYMQRKARKDGEIKELKNQETEEQTGGLSQNLVKLFWKLDIFGVILLTAGLGLILVPLTLAGG 240
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 299 TSQKWHNSKIIATLVSGGCLFFIFLYWEAKFAKSPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVLLVSMKESS 378
Cdd:cd17322 241 AKSKWKNSSIIAMIVLGGVLLVAFLLWEAKFAKYPFLPFRLLKDRTILAACLIDFFYFFSYYIFSDYLYTVLQVAVGESI 320
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 379 TSAARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKYRGGSGSHEGVIAASVIMGLSGLLCSNSV 458
Cdd:cd17322 321 TSATRITNLYSFVSTVAGIFVGFLIRKTRRYKVLVIIGVPLYIVGMGLLIRYRGGSGSHGGIIGALILIGIGGGLFTYPV 400
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 459 IVILQAMTTHSRMAVITGIQYTFSKLGAAIGASVSGAIWTQTMPNQLYKNLGNDTLAEIAYASPYTF-ISDYPWGSPERD 537
Cdd:cd17322 401 QVSVQASVSHQEMAVVTALLLTVYSIGGAVGAAISGAIWTQTLPKKLLKNLPDSLKATAAYIFPSTFvILSYPWGTPERD 480
                       490       500       510
                ....*....|....*....|....*....|....
gi 10383750 538 AVVESYRYVQRIIMTVGLACTVPFFAFTMFMRDP 571
Cdd:cd17322 481 AIIESYRYVQRILLIVALVFSAPMFALMFFLRNP 514
TRI12 pfam06609
Fungal trichothecene efflux pump (TRI12); This family consists of several fungal specific ...
95-580 8.81e-11

Fungal trichothecene efflux pump (TRI12); This family consists of several fungal specific trichothecene efflux pump proteins. Many of the genes involved in trichothecene toxin biosynthesis in Fusarium sporotrichioides are present within a gene cluster.It has been suggested that TRI12 may play a role in F. sporotrichioides self-protection against trichothecenes.


Pssm-ID: 115279 [Multi-domain]  Cd Length: 598  Bit Score: 64.69  E-value: 8.81e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750    95 SLLSTVQVINAVVSVgsqVVYSRLSDHFGRlRLFLVAT-IFYIMGTIIQSQATRL-TMYAAGSVF-YNCGYVGTNlllTL 171
Cdd:pfam06609  81 GLFSTLWTMGQAVSI---LMMGRLTDRFGR-RPFVIAThIIGLVGAIVGCTANKFnTLLAAMTLLgVAAGPAGAS---PL 153
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   172 ILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIamwaFIYPL-SALPIIFLILYmkYKSSKTAEWRS 250
Cdd:pfam06609 154 FIGELMSNKTKFLGLLIVSAPTIAMNGAGPYFGQRLAIQGNWRWIF----YIYIImSAIAVLLIIIW--YHPPSFAQLHG 227
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   251 LKEQARKErtgglfenlvflFWKLDIVGILLITVSLGCILVPLTLANETSQKWHNSKIIATLVSGGCLFFIFLYWEAkFA 330
Cdd:pfam06609 228 KKARKRDE------------LAKLDWIGIFLVIAGTSLFLLGVSWGGKPNNPWNSAKIIGLISSGAGSLVIFALYEV-FG 294
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   331 K--SPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVLLVSMKESS----TSAARIVNLPDFVAATASPFYSLLVA 404
Cdd:pfam06609 295 KpeQPIIPPALFKDTRGFVCILIISSIMGAMHLCLTIIYPQQVINIFGSSlknwEETAWMSATASFGTGAGVMILGNLFH 374
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   405 KTRKLKLSVIGGcAAWMVCmglfykYRGGSGS-----HEGVIAASVIMGLSGLLCSNSVIVILQAMTTHSRMAVITGIQY 479
Cdd:pfam06609 375 LIRHIRWQILVG-ALWLTA------FLGAMSSinrdnKNAAIALSFFSGFVVAWAQDITMLLVQFITTDEDLGVAFAVVA 447
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   480 TFSKLGAAIGASVSGAIWTQTMPNQLYKNL-----GND-------TLAEIAYASPYTFISDYPWGSPERDAVVE------ 541
Cdd:pfam06609 448 AARPFAGSIFTAAFISLYSNQYPKEIASHLssalrGTDfpqasfpSLLEAAKSGRIDAVKALPGMTNEIAAVVSqamads 527
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 10383750   542 ---SYRYVQRIIMTVGLactVPFFAfTMFMRDPE--LIDKATHE 580
Cdd:pfam06609 528 ytaSYANVYYFAMALGV---IPIIA-SLCMRDFDcyLTDHVPHQ 567
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
60-264 1.78e-05

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 47.28  E-value: 1.78e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  60 ILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTvqvINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMGT 139
Cdd:COG2814  13 LALALGAFLSGLGIGIVLPALPLIAADLGASPAQAGLVVT---AYLLGAALGAPLAGRLADRFGRRRVLLLGLLLFALGS 89
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 140 IIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRM----FYQYASYWPYIIIPWISGNIITAanpqknWSW 215
Cdd:COG2814  90 LLCALAPSLWLLLAARFLQGLGAGALFPAALALIADLVPPERRGralgLLGAGLGLGPALGPLLGGLLADL------FGW 163
                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|.
gi 10383750 216 NIAMWAFIyPLSALPIIFLILYMK--YKSSKTAEWRSLKEQARKERTGGLF 264
Cdd:COG2814 164 RWVFLVNA-VLALLALLLLLRLLPesRPAARARLRGSLRELLRRPRLLLLL 213
 
Name Accession Description Interval E-value
MFS_ARN_like cd17322
Yeast ARN family of Siderophore iron transporters and similar proteins of the Major ...
59-571 0e+00

Yeast ARN family of Siderophore iron transporters and similar proteins of the Major Facilitator Superfamily; The ARN family of siderophore iron transporters includes ARN1 (or ferrichrome permease), ARN2 (or triacetylfusarinine C transporter 1 or TAF1), ARN3 (or siderophore iron transporter 1 or SIT1 or ferrioxamine B permease) and ARN4 (or Enterobactin permease or ENB1). They specifically recognize siderophore-iron chelates are expressed under conditions of iron deprivation. They facilitate the uptake of both hydroxamate- and catecholate-type siderophores. This group also includes glutathione exchanger 1 (Gex1p) and Gex2p, which are proton/glutathione antiporters that import glutathione from the vacuole and exports it through the plasma membrane. The ARN family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340880 [Multi-domain]  Cd Length: 514  Bit Score: 684.42  E-value: 0e+00
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  59 KILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTVQVINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMG 138
Cdd:cd17322   1 LIILFFSLFLCGFAIGLDGQIRYSYQAYATSSFSKHSLLSTINVVNNIVSAVSQPPYAKLSDVFGRLELFLVSLLLYVVG 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 139 TIIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIA 218
Cdd:cd17322  81 TIIMAASTNVQTYAAGSVFYNAGYTGLNLLLTLILADFSSLKNRGFASAIPSLPYIITTWIGGNIAQAALPNSNWRWGYG 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 219 MWAFIYPLSALPIIFLILYMKYKSSKTAEWRSLKEQARKERTGGLFENLVFLFWKLDIVGILLITVSLGCILVPLTLANE 298
Cdd:cd17322 161 MWAIILPLSSLPLVLCLLYMQRKARKDGEIKELKNQETEEQTGGLSQNLVKLFWKLDIFGVILLTAGLGLILVPLTLAGG 240
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 299 TSQKWHNSKIIATLVSGGCLFFIFLYWEAKFAKSPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVLLVSMKESS 378
Cdd:cd17322 241 AKSKWKNSSIIAMIVLGGVLLVAFLLWEAKFAKYPFLPFRLLKDRTILAACLIDFFYFFSYYIFSDYLYTVLQVAVGESI 320
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 379 TSAARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYKYRGGSGSHEGVIAASVIMGLSGLLCSNSV 458
Cdd:cd17322 321 TSATRITNLYSFVSTVAGIFVGFLIRKTRRYKVLVIIGVPLYIVGMGLLIRYRGGSGSHGGIIGALILIGIGGGLFTYPV 400
                       410       420       430       440       450       460       470       480
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 459 IVILQAMTTHSRMAVITGIQYTFSKLGAAIGASVSGAIWTQTMPNQLYKNLGNDTLAEIAYASPYTF-ISDYPWGSPERD 537
Cdd:cd17322 401 QVSVQASVSHQEMAVVTALLLTVYSIGGAVGAAISGAIWTQTLPKKLLKNLPDSLKATAAYIFPSTFvILSYPWGTPERD 480
                       490       500       510
                ....*....|....*....|....*....|....
gi 10383750 538 AVVESYRYVQRIIMTVGLACTVPFFAFTMFMRDP 571
Cdd:cd17322 481 AIIESYRYVQRILLIVALVFSAPMFALMFFLRNP 514
MFS_TRI12_like cd06179
Fungal trichothecene efflux pump (TRI12) of the Major Facilitator Superfamily of transporters; ...
99-510 6.13e-30

Fungal trichothecene efflux pump (TRI12) of the Major Facilitator Superfamily of transporters; This family includes Fusarium sporotrichioides trichothecene efflux pump (TRI12), which may play a role in F. sporotrichioides self-protection against trichothecenes. TRI12 belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340868 [Multi-domain]  Cd Length: 518  Bit Score: 123.89  E-value: 6.13e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  99 TVQVINAVVSVGSqVVYSRLSDHFGRLRLFLVATIFYIMGTIIQSQATRLTMYAAGSVFYNCGYvGTNLLLTLILSDFSS 178
Cdd:cd06179  37 ISTAWSLAQAVLG-PIVGRLSDLFGRRWFFIGGNLLGVVGSIVAGTAKSVNMLIGGQALIGIGA-GTQGLGAAVASELVP 114
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 179 LKWRMFYQYASYWPYIIIPWISGNIITAA--NPQKNWSWNIAMWAFIYPLSAlpIIFLILYmKYKSSKTAEWRSLKEQar 256
Cdd:cd06179 115 NKYRGLVQGVVNLVASPGSALGPLIAGALveANAAGWRWIFYINAIFNALAL--ILLFFFY-HPPPRPQLHGSSTRQK-- 189
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 257 kertgglfenlvflFWKLDIVGILLITVSLGCILVPLTLANeTSQKWHNSKIIATLVSGGCLFFIFLYWEAKFAKSPLLP 336
Cdd:cd06179 190 --------------LKRLDWVGLFLLIAGLVLFLLGLSWGG-NPYPWSSAHVIAPLVIGGVLLIAFGLYEWKGTKDGLLP 254
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 337 FKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVLLVSMKESSTSAARIVNLPDFVAAT-ASPFYSLLVAKTRKLKLSVIG 415
Cdd:cd06179 255 PRLLFRGRNFALILIVAFVEGMIFYSLNIFWPQQISLLFTTDPLKAGLYQLPFGLGTLfGAILAGLLLTKIKHIKWQLVA 334
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 416 GCAAWMVCMGLFYKYRGGSGSHegVIAASVI--MGLSGLLCsnSVIVILQAMTTHSRMAVITGIQYTFSKLGAAIGASVS 493
Cdd:cd06179 335 GFVLFTAFLALLALITPDNKAL--AIAFSVLagIGIGGPLV--LAIVIVQLSVPPEDIGTASGLLGSARALGGAVGTAIF 410
                       410
                ....*....|....*..
gi 10383750 494 GAIWTQTMPNQLYKNLG 510
Cdd:cd06179 411 TSILSNRLSKNLPARVA 427
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
66-500 4.26e-11

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 65.14  E-value: 4.26e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  66 AFVCGFGISLDYTLRSTYTGYATNSYS-EHSLLSTVQVINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMGTIIQSQ 144
Cdd:cd06174   1 LLLGFFLTGLARGLISPLLPALLQSFGlSASQLGLLFALFSLGYALLQPLAGLLADRFGRRPVLLLGLLLFALGALLFAF 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 145 ATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRM----FYQYASYWPYIIIPWISGNIITAAnpQKNWSWNIAMW 220
Cdd:cd06174  81 APSFWLLLLGRFLLGLGSGLIDPAVLALIADLFPERERGralgLLQAFGSVGGILGPLLGGILASSL--GFGWRAVFLIA 158
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 221 AFIYPLSALPIIFLILYMKYKSSKTAEWRSLKEQARkertgglfenLVFLFWKLDIVGILLItvslgcilvpltlanets 300
Cdd:cd06174 159 AALALLAAILLLLVVPDPPESARAKNEEASSKSVLK----------LLKRVLKNPGLWLLLL------------------ 210
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 301 qkwhnskiiatlvsggclfFIFLYWEAKFAKSPLLPFKLLSDRGIwaplgvtffnfftffiscdylypvllvsmkeSSTS 380
Cdd:cd06174 211 -------------------AIFLVNLAYYSFSTLLPLFLLDLGGL-------------------------------SVAV 240
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 381 AARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFYkyrgGSGSHEGVIAASVIMGLSGLLCSNSVIV 460
Cdd:cd06174 241 AGLLLSLFGLAGALGSLLLGLLSDRLIGRKPLLLIGLLLMALGLALLL----LAPSLLLLLLLLLLLGFGLGGLLPLSFA 316
                       410       420       430       440
                ....*....|....*....|....*....|....*....|
gi 10383750 461 ILQAMTTHSRMAVITGIQYTFSKLGAAIGASVSGAIWTQT 500
Cdd:cd06174 317 LIAELFPPEIRGTAFGLLNTFGFLGGAIGPLLAGFLLAAT 356
TRI12 pfam06609
Fungal trichothecene efflux pump (TRI12); This family consists of several fungal specific ...
95-580 8.81e-11

Fungal trichothecene efflux pump (TRI12); This family consists of several fungal specific trichothecene efflux pump proteins. Many of the genes involved in trichothecene toxin biosynthesis in Fusarium sporotrichioides are present within a gene cluster.It has been suggested that TRI12 may play a role in F. sporotrichioides self-protection against trichothecenes.


Pssm-ID: 115279 [Multi-domain]  Cd Length: 598  Bit Score: 64.69  E-value: 8.81e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750    95 SLLSTVQVINAVVSVgsqVVYSRLSDHFGRlRLFLVAT-IFYIMGTIIQSQATRL-TMYAAGSVF-YNCGYVGTNlllTL 171
Cdd:pfam06609  81 GLFSTLWTMGQAVSI---LMMGRLTDRFGR-RPFVIAThIIGLVGAIVGCTANKFnTLLAAMTLLgVAAGPAGAS---PL 153
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   172 ILSDFSSLKWRMFYQYASYWPYIIIPWISGNIITAANPQKNWSWNIamwaFIYPL-SALPIIFLILYmkYKSSKTAEWRS 250
Cdd:pfam06609 154 FIGELMSNKTKFLGLLIVSAPTIAMNGAGPYFGQRLAIQGNWRWIF----YIYIImSAIAVLLIIIW--YHPPSFAQLHG 227
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   251 LKEQARKErtgglfenlvflFWKLDIVGILLITVSLGCILVPLTLANETSQKWHNSKIIATLVSGGCLFFIFLYWEAkFA 330
Cdd:pfam06609 228 KKARKRDE------------LAKLDWIGIFLVIAGTSLFLLGVSWGGKPNNPWNSAKIIGLISSGAGSLVIFALYEV-FG 294
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   331 K--SPLLPFKLLSDRGIWAPLGVTFFNFFTFFISCDYLYPVLLVSMKESS----TSAARIVNLPDFVAATASPFYSLLVA 404
Cdd:pfam06609 295 KpeQPIIPPALFKDTRGFVCILIISSIMGAMHLCLTIIYPQQVINIFGSSlknwEETAWMSATASFGTGAGVMILGNLFH 374
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   405 KTRKLKLSVIGGcAAWMVCmglfykYRGGSGS-----HEGVIAASVIMGLSGLLCSNSVIVILQAMTTHSRMAVITGIQY 479
Cdd:pfam06609 375 LIRHIRWQILVG-ALWLTA------FLGAMSSinrdnKNAAIALSFFSGFVVAWAQDITMLLVQFITTDEDLGVAFAVVA 447
                         410       420       430       440       450       460       470       480
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   480 TFSKLGAAIGASVSGAIWTQTMPNQLYKNL-----GND-------TLAEIAYASPYTFISDYPWGSPERDAVVE------ 541
Cdd:pfam06609 448 AARPFAGSIFTAAFISLYSNQYPKEIASHLssalrGTDfpqasfpSLLEAAKSGRIDAVKALPGMTNEIAAVVSqamads 527
                         490       500       510       520
                  ....*....|....*....|....*....|....*....|....
gi 10383750   542 ---SYRYVQRIIMTVGLactVPFFAfTMFMRDPE--LIDKATHE 580
Cdd:pfam06609 528 ytaSYANVYYFAMALGV---IPIIA-SLCMRDFDcyLTDHVPHQ 567
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
60-264 1.78e-05

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 47.28  E-value: 1.78e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  60 ILLISTAFVCGFGISLDYTLRSTYTGYATNSYSEHSLLSTvqvINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMGT 139
Cdd:COG2814  13 LALALGAFLSGLGIGIVLPALPLIAADLGASPAQAGLVVT---AYLLGAALGAPLAGRLADRFGRRRVLLLGLLLFALGS 89
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 140 IIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRM----FYQYASYWPYIIIPWISGNIITAanpqknWSW 215
Cdd:COG2814  90 LLCALAPSLWLLLAARFLQGLGAGALFPAALALIADLVPPERRGralgLLGAGLGLGPALGPLLGGLLADL------FGW 163
                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|.
gi 10383750 216 NIAMWAFIyPLSALPIIFLILYMK--YKSSKTAEWRSLKEQARKERTGGLF 264
Cdd:COG2814 164 RWVFLVNA-VLALLALLLLLRLLPesRPAARARLRGSLRELLRRPRLLLLL 213
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
39-257 5.79e-05

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 46.05  E-value: 5.79e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  39 TFFKLKENEIMSAQFDSLK--YKILLISTAF----VCGFGISLDYTLRSTYTGYATNSY--SEHSLLSTVQVINAVVSVG 110
Cdd:COG2211 200 TFFGTKERPVPEEEKVSLKesLKALLKNRPFllllLAYLLFFLALALVAALLLYYFKYVlgLSAALVGLLLALYFLAALL 279
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 111 SQVVYSRLSDHFGRLRLFLVATIFYIMGTIIQ--SQATRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSL-KWRMFYQ- 186
Cdd:COG2211 280 GAPLWPRLAKRFGKKKAFIIGLLLAALGLLLLffLGPGNLWLLLVLAALAGIGLGAILVLPWAMLADVVDYdEWKTGRRr 359
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 187 ---YASYW------PYIIIPWISGNIITAANPQKNWSWNI-AMWAFIYPLSALPIIF----LILYMKYKSSKTAEWRSLK 252
Cdd:COG2211 360 eglYFGIFtfaiklGQALAGALAGLLLALFGYVAGAAQSPsALTGIRLLFFLLPAVLlllaALLLLFYPLTRERHAEIRA 439

                ....*
gi 10383750 253 EQARK 257
Cdd:COG2211 440 ELAAR 444
MFS_MMR_MDR_like cd17504
Methylenomycin A resistance protein (also called MMR peptide)-like multidrug resistance (MDR) ...
332-498 1.34e-04

Methylenomycin A resistance protein (also called MMR peptide)-like multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This subfamily is composed of putative multidrug resistance (MDR) transporters including Chlamydia trachomatis antiseptic resistance protein QacA_2, and Serratia sp. DD3 Bmr3. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. This subfamily belongs to the Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters (MMR-like MDR transporter) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341047 [Multi-domain]  Cd Length: 371  Bit Score: 44.49  E-value: 1.34e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 332 SPLLPFKLLSDRGIWAPLGVTFFNFFTFFIScDYLYPVLLVSMKE----SSTSAARIVNLPDFVAAT-ASPFYSLLVAKT 406
Cdd:cd17504 166 VPLIDLRLLRRRNVLLANITALLAGFGMFMV-FQLLPQLLQLPTPtgfgLGITEAGLLLLPGALVMLiAGPLAGRLIARV 244
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 407 RKLKLSVIGGCAAWMVCMGLFYKyrggSGSHEGVIAASVIMGLSGLLCSNSVIVILQAMTTHSRMAVITGIQYTFSKLGA 486
Cdd:cd17504 245 GPRRVLLIGLVISAVGLLLLALL----HSSLWALVVALLLIGIGLGLAFASLPNIVVESVPPDRTGIATGMNTVLRTIGS 320
                       170
                ....*....|..
gi 10383750 487 AIGASVSGAIWT 498
Cdd:cd17504 321 AIGPAIAGAILT 332
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
50-175 2.07e-03

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 40.73  E-value: 2.07e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  50 SAQFDSLKYKILLISTAFVCGFGISLdYTLRSTYTGYATNSYS-EHSLLSTVQVINAVVSVGSQVVYSRLSDHFGRLRLF 128
Cdd:COG2814 198 GSLRELLRRPRLLLLLLLAFLLGFGF-FALFTYLPLYLQEVLGlSASAAGLLLALFGLGGVLGALLAGRLADRFGRRRLL 276
                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*..
gi 10383750 129 LVATIFYIMGTIIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILSD 175
Cdd:COG2814 277 LIGLLLLALGLLLLALAGSLWLLLLALFLLGFGFGLLFPLLQALVAE 323
MFS_1 pfam07690
Major Facilitator Superfamily;
71-490 2.97e-03

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 40.09  E-value: 2.97e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750    71 FGISLDYTLRSTYTGYATNSYSEH-----SLLSTVQVINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYIMGTIIQSQA 145
Cdd:pfam07690   3 LAAFLAALGRSLLGPALPLLLAEDlgispTEIGLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLLLFALGLLLLLFA 82
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   146 TRLTMYAAGSVFYNCGYVGTNLLLTLILSDFSSLKWRM----FYQYASYWPYIIIPWISGNIITaanpqkNWSWNIAMWA 221
Cdd:pfam07690  83 SSLWLLLVLRVLQGLGAGALFPAALALIADWFPPEERGralgLVSAGFGLGAALGPLLGGLLAS------LFGWRAAFLI 156
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   222 FIyPLSALPIIFLILYMKYKSSKTAEWRSLKEQARKERTGGLFENLVFLfwkldivgillitvslgcilvpltlanetsq 301
Cdd:pfam07690 157 LA-ILSLLAAVLLLLPRPPPESKRPKPAEEARLSLIVAWKALLRDPVLW------------------------------- 204
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   302 kwhnskIIATLVSGGCLFFIFLYWeakfakspllpfkllsdrgiwaplgvtffnfftffiscdylYPVLLVSMKESSTSA 381
Cdd:pfam07690 205 ------LLLALLLFGFAFFGLLTY-----------------------------------------LPLYQEVLGLSALLA 237
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750   382 ARIVNLPDFVAATASPFYSLLVAKTRKLKLSVIGGCAAWMVCMGLFykYRGGSGSHEGVIAASVIMGLSGLLCSNSVIVI 461
Cdd:pfam07690 238 GLLLGLGGLLGAIGRLLLGRLSDRLGRRRRLLLALLLLILAALGLL--LLSLTLSSLWLLLALLLLGFGFGLVFPALNAL 315
                         410       420
                  ....*....|....*....|....*....
gi 10383750   462 LQAMTTHSRMAVITGIQYTFSKLGAAIGA 490
Cdd:pfam07690 316 VSDLAPKEERGTASGLYNTAGSLGGALGP 344
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
276-498 3.78e-03

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 40.27  E-value: 3.78e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 276 IVGILLITVSLGcILVPLtLANETSQKWHNSKIIATLVSGGCLFFIFLY-WEAKFAKSPLLPFK-----LLSDRGIWAPL 349
Cdd:COG2211 157 GLGGLLASVLPP-PLVAA-FGGDAALGYRLTALIFAVLGLLAFLLTFFGtKERPVPEEEKVSLKeslkaLLKNRPFLLLL 234
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 350 GVTFFNFFTFFISCDYLYPVLLVSMKESSTSAArIVNLPDFVAATAS-PFYSLLVAKTRKLKLsVIGGCAAWMVCMGLFY 428
Cdd:COG2211 235 LAYLLFFLALALVAALLLYYFKYVLGLSAALVG-LLLALYFLAALLGaPLWPRLAKRFGKKKA-FIIGLLLAALGLLLLF 312
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750 429 KYRGGSgshegVIAASVIMGLSGLlCSNSVIVILQAM-----------TTHSRMAVITGIQYTFSKLGAAIGASVSGAIW 497
Cdd:COG2211 313 FLGPGN-----LWLLLVLAALAGI-GLGAILVLPWAMladvvdydewkTGRRREGLYFGIFTFAIKLGQALAGALAGLLL 386

                .
gi 10383750 498 T 498
Cdd:COG2211 387 A 387
MFS_SLC22 cd17317
Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters of the Major ...
96-238 8.38e-03

Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters of the Major Facilitator Superfamily; The Solute carrier 22 (SLC22) family of organic cation/anion/zwitterion transporters includes organic cation transporters (OCTs), organic zwitterion/cation transporters (OCTNs), and organic anion transporters (OATs). SLC22 transporters interact with a variety of compounds that include drugs of abuse, environmental toxins, opioid analgesics, antidepressant and anxiolytic agents, and neurotransmitters and their metabolites. The SLC22 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340875 [Multi-domain]  Cd Length: 331  Bit Score: 38.72  E-value: 8.38e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383750  96 LLSTVQVINAV-VSVGSqVVYSRLSDHFGRLRLFLVATIFYIMGTIIQSQATRLTMYAAGSVFYNCGYVGTNLLLTLILS 174
Cdd:cd17317  26 LVALVQTIFFLgVLIGS-LLFGYLADRFGRRPVLLLGLVLQIVCGVLSAFSPSYELFLVLRFLVGVTSAGIFTVGFVLGM 104
                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 10383750 175 DFSSLKWRMFyqyASYwpYIIIPWISGNIITAAnpqknWSWNIAMWAFIYPLSALPIIFLILYM 238
Cdd:cd17317 105 EIVGPKYRSF---VGI--LFGLFWALGYMLLAL-----LAYLIRDWRWLQLAISLPGLLFLLLL 158
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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