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Conserved domains on  [gi|10383799|ref|NP_009967|]
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Ras family guanine nucleotide exchange factor BUD5 [Saccharomyces cerevisiae S288C]

Protein Classification

Ras-GEF domain-containing protein( domain architecture ID 11260643)

Ras guanine nucleotide exchange factor (Ras-GEF) domain-containing protein activates Ras-like small GTPases by mediating the replacement of GDP with GTP

Gene Ontology:  GO:0007264|GO:0005085
PubMed:  7786285
SCOP:  4001268|4000381

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
409-640 2.94e-58

Guanine nucleotide exchange factor for Ras-like small GTPases;


:

Pssm-ID: 214539  Cd Length: 242  Bit Score: 195.93  E-value: 2.94e-58
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    409 LNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDT-------TIDSVFTLSNQLSSYVLETTLQQTH------TISYW 475
Cdd:smart00147   2 LLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSkkspsplNLEAFIRRFNEVSNWVATEILKQTTpkdraeLLSKF 81
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    476 LQVALSCLYLRNLNSLASIITSLQNHSIERLSLPIDV---KSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSqlPCVPFT 552
Cdd:smart00147  82 IQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKlpsKYKKLFEELEELLSPERNYKNYREALSSCNLP--PCIPFL 159
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    553 SLLIRDITFIRDGNDTFTKDgNNVNMQKFNQITKIVAFAQYLQQkQYEDIHCSNTTARSLLGAMIkvHTLYNDNKdrAYQ 632
Cdd:smart00147 160 GVLLKDLTFIDEGNPDFLEN-GLVNFEKRRQIAEILREIRQLQS-QPYNLRPNRSDIQSLLQQLL--DHLDEEEE--LYQ 233

                   ....*...
gi 10383799    633 VSIAKVPR 640
Cdd:smart00147 234 LSLKIEPR 241
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
230-339 6.47e-22

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


:

Pssm-ID: 214571  Cd Length: 127  Bit Score: 91.63  E-value: 6.47e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    230 LYLNKMLPHNSHKWQKPSPFDSANLLLNFRDWTTDNALLQELLLSYPTINKNKHKN---------HSVPRLIQIWVESYW 300
Cdd:smart00229   8 GTLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPESWVEekvnprrvkNRVLNILRTWVENYW 87
                           90       100       110       120
                   ....*....|....*....|....*....|....*....|
gi 10383799    301 QDSETTLKDILNFW-YSHLAEYYEYQELFADIVQLFINKK 339
Cdd:smart00229  88 EDFEDDPKLISFLLeFLELVDDEKYPGLVTSLLNLLRRLS 127
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
409-640 2.94e-58

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 195.93  E-value: 2.94e-58
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    409 LNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDT-------TIDSVFTLSNQLSSYVLETTLQQTH------TISYW 475
Cdd:smart00147   2 LLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSkkspsplNLEAFIRRFNEVSNWVATEILKQTTpkdraeLLSKF 81
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    476 LQVALSCLYLRNLNSLASIITSLQNHSIERLSLPIDV---KSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSqlPCVPFT 552
Cdd:smart00147  82 IQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKlpsKYKKLFEELEELLSPERNYKNYREALSSCNLP--PCIPFL 159
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    553 SLLIRDITFIRDGNDTFTKDgNNVNMQKFNQITKIVAFAQYLQQkQYEDIHCSNTTARSLLGAMIkvHTLYNDNKdrAYQ 632
Cdd:smart00147 160 GVLLKDLTFIDEGNPDFLEN-GLVNFEKRRQIAEILREIRQLQS-QPYNLRPNRSDIQSLLQQLL--DHLDEEEE--LYQ 233

                   ....*...
gi 10383799    633 VSIAKVPR 640
Cdd:smart00147 234 LSLKIEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
409-635 3.65e-58

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 195.55  E-value: 3.65e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799 409 LNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDT-------TIDSVFTLSNQLSSYVLETTLQQT------HTISYW 475
Cdd:cd00155   2 LSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDknihlspNLERFIERFNNLSNWVASEILLCTnpkkraRLLSKF 81
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799 476 LQVALSCLYLRNLNSLASIITSLQNHSIERLSLPIDV---KSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSQlPCVPFT 552
Cdd:cd00155  82 IQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVlssKLKKLFEELEELVDPSRNFKNYRKLLKSVGPNP-PCVPFL 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799 553 SLLIRDITFIRDGNDTFTKdGNNVNMQKFNQITKIVAFAQYLQQKQYEDIHcsnttARSLLGAMIKVHTLYnDNKDRAYQ 632
Cdd:cd00155 161 GVYLKDLTFLHEGNPDFLE-GNLVNFEKRRKIAEILREIRQLQSNSYELNR-----DEDILAFLWKLLELI-LNEDELYE 233

                ...
gi 10383799 633 VSI 635
Cdd:cd00155 234 LSL 236
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
416-581 5.00e-36

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 133.49  E-value: 5.00e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799   416 LAKTLTLLESSLYLDIETIEF------TRHFKHNDTTIDSVFTLSNQLSSYVLETTLQQT------HTISYWLQVALSCL 483
Cdd:pfam00617   2 LARQLTLIEFELFRKIKPRELlgsawsKKDKKENSPNIEAMIARFNKLSNWVASEILSEEdlkkraKVIKKFIKIAEHCR 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799   484 YLRNLNSLASIITSLQNHSIERLSLP---IDVKSDHLFQRLKVVVHPNNNYNVYRRTIKHIfhsQLPCVPFTSLLIRDIT 560
Cdd:pfam00617  82 ELNNFNSLMAILSGLNSSPISRLKKTwelVSKKYKKTLEELEKLMSPSRNFKNYREALSSA---SPPCIPFLGLYLTDLT 158
                         170       180
                  ....*....|....*....|.
gi 10383799   561 FIRDGNDTFTKDGnNVNMQKF 581
Cdd:pfam00617 159 FIEEGNPDFLEGG-LINFEKR 178
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
230-339 6.47e-22

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 91.63  E-value: 6.47e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    230 LYLNKMLPHNSHKWQKPSPFDSANLLLNFRDWTTDNALLQELLLSYPTINKNKHKN---------HSVPRLIQIWVESYW 300
Cdd:smart00229   8 GTLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPESWVEekvnprrvkNRVLNILRTWVENYW 87
                           90       100       110       120
                   ....*....|....*....|....*....|....*....|
gi 10383799    301 QDSETTLKDILNFW-YSHLAEYYEYQELFADIVQLFINKK 339
Cdd:smart00229  88 EDFEDDPKLISFLLeFLELVDDEKYPGLVTSLLNLLRRLS 127
 
Name Accession Description Interval E-value
RasGEF smart00147
Guanine nucleotide exchange factor for Ras-like small GTPases;
409-640 2.94e-58

Guanine nucleotide exchange factor for Ras-like small GTPases;


Pssm-ID: 214539  Cd Length: 242  Bit Score: 195.93  E-value: 2.94e-58
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    409 LNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDT-------TIDSVFTLSNQLSSYVLETTLQQTH------TISYW 475
Cdd:smart00147   2 LLLDPKELAEQLTLLDFELFRKIDPSELLGSVWGKRSkkspsplNLEAFIRRFNEVSNWVATEILKQTTpkdraeLLSKF 81
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    476 LQVALSCLYLRNLNSLASIITSLQNHSIERLSLPIDV---KSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSqlPCVPFT 552
Cdd:smart00147  82 IQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEKlpsKYKKLFEELEELLSPERNYKNYREALSSCNLP--PCIPFL 159
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    553 SLLIRDITFIRDGNDTFTKDgNNVNMQKFNQITKIVAFAQYLQQkQYEDIHCSNTTARSLLGAMIkvHTLYNDNKdrAYQ 632
Cdd:smart00147 160 GVLLKDLTFIDEGNPDFLEN-GLVNFEKRRQIAEILREIRQLQS-QPYNLRPNRSDIQSLLQQLL--DHLDEEEE--LYQ 233

                   ....*...
gi 10383799    633 VSIAKVPR 640
Cdd:smart00147 234 LSLKIEPR 241
RasGEF cd00155
Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of ...
409-635 3.65e-58

Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. Guanine-nucleotide-exchange factors (GEFs) positively regulate these GTP-binding proteins in response to a variety of signals. GEFs catalyze the dissociation of GDP from the inactive GTP-binding proteins. GTP can then bind and induce structural changes that allow interaction with effectors.


Pssm-ID: 238087 [Multi-domain]  Cd Length: 237  Bit Score: 195.55  E-value: 3.65e-58
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799 409 LNVSPWSLAKTLTLLESSLYLDIETIEFTRHFKHNDT-------TIDSVFTLSNQLSSYVLETTLQQT------HTISYW 475
Cdd:cd00155   2 LSLDPKELAEQLTLLDFELFRKIEPFELLGSLWSKKDknihlspNLERFIERFNNLSNWVASEILLCTnpkkraRLLSKF 81
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799 476 LQVALSCLYLRNLNSLASIITSLQNHSIERLSLPIDV---KSDHLFQRLKVVVHPNNNYNVYRRTIKHIFHSQlPCVPFT 552
Cdd:cd00155  82 IQVAKHCRELNNFNSLMAIVSALSSSPISRLKKTWEVlssKLKKLFEELEELVDPSRNFKNYRKLLKSVGPNP-PCVPFL 160
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799 553 SLLIRDITFIRDGNDTFTKdGNNVNMQKFNQITKIVAFAQYLQQKQYEDIHcsnttARSLLGAMIKVHTLYnDNKDRAYQ 632
Cdd:cd00155 161 GVYLKDLTFLHEGNPDFLE-GNLVNFEKRRKIAEILREIRQLQSNSYELNR-----DEDILAFLWKLLELI-LNEDELYE 233

                ...
gi 10383799 633 VSI 635
Cdd:cd00155 234 LSL 236
RasGEF pfam00617
RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.
416-581 5.00e-36

RasGEF domain; Guanine nucleotide exchange factor for Ras-like small GTPases.


Pssm-ID: 459872  Cd Length: 179  Bit Score: 133.49  E-value: 5.00e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799   416 LAKTLTLLESSLYLDIETIEF------TRHFKHNDTTIDSVFTLSNQLSSYVLETTLQQT------HTISYWLQVALSCL 483
Cdd:pfam00617   2 LARQLTLIEFELFRKIKPRELlgsawsKKDKKENSPNIEAMIARFNKLSNWVASEILSEEdlkkraKVIKKFIKIAEHCR 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799   484 YLRNLNSLASIITSLQNHSIERLSLP---IDVKSDHLFQRLKVVVHPNNNYNVYRRTIKHIfhsQLPCVPFTSLLIRDIT 560
Cdd:pfam00617  82 ELNNFNSLMAILSGLNSSPISRLKKTwelVSKKYKKTLEELEKLMSPSRNFKNYREALSSA---SPPCIPFLGLYLTDLT 158
                         170       180
                  ....*....|....*....|.
gi 10383799   561 FIRDGNDTFTKDGnNVNMQKF 581
Cdd:pfam00617 159 FIEEGNPDFLEGG-LINFEKR 178
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
230-339 6.47e-22

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 91.63  E-value: 6.47e-22
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 10383799    230 LYLNKMLPHNSHKWQKPSPFDSANLLLNFRDWTTDNALLQELLLSYPTINKNKHKN---------HSVPRLIQIWVESYW 300
Cdd:smart00229   8 GTLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPESWVEekvnprrvkNRVLNILRTWVENYW 87
                           90       100       110       120
                   ....*....|....*....|....*....|....*....|
gi 10383799    301 QDSETTLKDILNFW-YSHLAEYYEYQELFADIVQLFINKK 339
Cdd:smart00229  88 EDFEDDPKLISFLLeFLELVDDEKYPGLVTSLLNLLRRLS 127
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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