Helix located at the middle N-terminal region of Sfi1p; Sfi1p is a single continuous alpha ...
218-306
2.51e-52
Helix located at the middle N-terminal region of Sfi1p; Sfi1p is a single continuous alpha helix wrapped with centrins, which are calmodulin-like proteins present in centrosomes and spindle pole bodies (SPBs). Sfi1p is localized to the half-bridge of the yeast SPBs, and like Cdc31p (yeast centrin), it is required for the early steps of SPB duplication. Sfi1p contains a central domain composed of multiple conserved repeats which all can bind to Cdc31p. The Sfi1p C-terminus has an important role in separation or splitting of the duplicated SPBs during bipolar spindle assembly.
:
Pssm-ID: 439387 Cd Length: 89 Bit Score: 177.61 E-value: 2.51e-52
Spindle body associated protein C-terminus; This C-terminal domain of spindle-body-associated ...
812-913
2.12e-36
Spindle body associated protein C-terminus; This C-terminal domain of spindle-body-associated protein Sfi1 has an important role to play in the bridge-splitting during bi-polar spindle assembly, and this separation event possibly requires interaction with integral components of the nuclear envelope, such as the Mps2-Bbp1 complex. Centrally to this domain is a region carrying centrin-binding repeats with repeating units containing tryptophan, family Sfi1_central, pfam08457.
The actual alignment was detected with superfamily member pfam10638:
Pssm-ID: 402327 Cd Length: 100 Bit Score: 132.52 E-value: 2.12e-36
Sfi1 spindle body protein; This is a family of fungal spindle pole body proteins that play a ...
259-794
9.99e-13
Sfi1 spindle body protein; This is a family of fungal spindle pole body proteins that play a role in spindle body duplication. They contain binding sites for calmodulin-like proteins called centrins which are present in microtubule-organizing centres.
The actual alignment was detected with superfamily member pfam08457:
Pssm-ID: 430007 [Multi-domain] Cd Length: 570 Bit Score: 71.94 E-value: 9.99e-13
Helix located at the middle N-terminal region of Sfi1p; Sfi1p is a single continuous alpha ...
218-306
2.51e-52
Helix located at the middle N-terminal region of Sfi1p; Sfi1p is a single continuous alpha helix wrapped with centrins, which are calmodulin-like proteins present in centrosomes and spindle pole bodies (SPBs). Sfi1p is localized to the half-bridge of the yeast SPBs, and like Cdc31p (yeast centrin), it is required for the early steps of SPB duplication. Sfi1p contains a central domain composed of multiple conserved repeats which all can bind to Cdc31p. The Sfi1p C-terminus has an important role in separation or splitting of the duplicated SPBs during bipolar spindle assembly.
Pssm-ID: 439387 Cd Length: 89 Bit Score: 177.61 E-value: 2.51e-52
Spindle body associated protein C-terminus; This C-terminal domain of spindle-body-associated ...
812-913
2.12e-36
Spindle body associated protein C-terminus; This C-terminal domain of spindle-body-associated protein Sfi1 has an important role to play in the bridge-splitting during bi-polar spindle assembly, and this separation event possibly requires interaction with integral components of the nuclear envelope, such as the Mps2-Bbp1 complex. Centrally to this domain is a region carrying centrin-binding repeats with repeating units containing tryptophan, family Sfi1_central, pfam08457.
Pssm-ID: 402327 Cd Length: 100 Bit Score: 132.52 E-value: 2.12e-36
Sfi1 spindle body protein; This is a family of fungal spindle pole body proteins that play a ...
259-794
9.99e-13
Sfi1 spindle body protein; This is a family of fungal spindle pole body proteins that play a role in spindle body duplication. They contain binding sites for calmodulin-like proteins called centrins which are present in microtubule-organizing centres.
Pssm-ID: 430007 [Multi-domain] Cd Length: 570 Bit Score: 71.94 E-value: 9.99e-13
Helix located at the middle N-terminal region of Sfi1p; Sfi1p is a single continuous alpha ...
218-306
2.51e-52
Helix located at the middle N-terminal region of Sfi1p; Sfi1p is a single continuous alpha helix wrapped with centrins, which are calmodulin-like proteins present in centrosomes and spindle pole bodies (SPBs). Sfi1p is localized to the half-bridge of the yeast SPBs, and like Cdc31p (yeast centrin), it is required for the early steps of SPB duplication. Sfi1p contains a central domain composed of multiple conserved repeats which all can bind to Cdc31p. The Sfi1p C-terminus has an important role in separation or splitting of the duplicated SPBs during bipolar spindle assembly.
Pssm-ID: 439387 Cd Length: 89 Bit Score: 177.61 E-value: 2.51e-52
Spindle body associated protein C-terminus; This C-terminal domain of spindle-body-associated ...
812-913
2.12e-36
Spindle body associated protein C-terminus; This C-terminal domain of spindle-body-associated protein Sfi1 has an important role to play in the bridge-splitting during bi-polar spindle assembly, and this separation event possibly requires interaction with integral components of the nuclear envelope, such as the Mps2-Bbp1 complex. Centrally to this domain is a region carrying centrin-binding repeats with repeating units containing tryptophan, family Sfi1_central, pfam08457.
Pssm-ID: 402327 Cd Length: 100 Bit Score: 132.52 E-value: 2.12e-36
Sfi1 spindle body protein; This is a family of fungal spindle pole body proteins that play a ...
259-794
9.99e-13
Sfi1 spindle body protein; This is a family of fungal spindle pole body proteins that play a role in spindle body duplication. They contain binding sites for calmodulin-like proteins called centrins which are present in microtubule-organizing centres.
Pssm-ID: 430007 [Multi-domain] Cd Length: 570 Bit Score: 71.94 E-value: 9.99e-13
Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01
References:
Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
of the residues that compose this conserved feature have been mapped to the query sequence.
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of your query sequence and the protein sequences used to curate the domain model,
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The thumbnail image, if present, provides an approximate view of the feature's location in 3 dimensions.
Click on the triangle for interactive 3D structure viewing options.
Functional characterization of the conserved domain architecture found on the query.
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This image shows a graphical summary of conserved domains identified on the query sequence.
The Show Concise/Full Display button at the top of the page can be used to select the desired level of detail: only top scoring hits
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Domains are color coded according to superfamilies
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Others (non-specific hits) and
superfamily placeholders are drawn in pastel colors.
if a domain or superfamily has been annotated with functional sites (conserved features),
they are mapped to the query sequence and indicated through sets of triangles
with the same color and shade of the domain or superfamily that provides the annotation. Mouse over the colored bars or triangles to see descriptions of the domains and features.
click on the bars or triangles to view your query sequence embedded in a multiple sequence alignment of the proteins used to develop the corresponding domain model.
The table lists conserved domains identified on the query sequence. Click on the plus sign (+) on the left to display full descriptions, alignments, and scores.
Click on the domain model's accession number to view the multiple sequence alignment of the proteins used to develop the corresponding domain model.
To view your query sequence embedded in that multiple sequence alignment, click on the colored bars in the Graphical Summary portion of the search results page,
or click on the triangles, if present, that represent functional sites (conserved features)
mapped to the query sequence.
Concise Display shows only the best scoring domain model, in each hit category listed below except non-specific hits, for each region on the query sequence.
(labeled illustration) Standard Display shows only the best scoring domain model from each source, in each hit category listed below for each region on the query sequence.
(labeled illustration) Full Display shows all domain models, in each hit category below, that meet or exceed the RPS-BLAST threshold for statistical significance.
(labeled illustration) Four types of hits can be shown, as available,
for each region on the query sequence:
specific hits meet or exceed a domain-specific e-value threshold
(illustrated example)
and represent a very high confidence that the query sequence belongs to the same protein family as the sequences use to create the domain model
non-specific hits
meet or exceed the RPS-BLAST threshold for statistical significance (default E-value cutoff of 0.01, or an E-value selected by user via the
advanced search options)
the domain superfamily to which the specific and non-specific hits belong
multi-domain models that were computationally detected and are likely to contain multiple single domains
Retrieve proteins that contain one or more of the domains present in the query sequence, using the Conserved Domain Architecture Retrieval Tool
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