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Conserved domains on  [gi|1316032111|ref|NP_077016|]
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glutathione-specific gamma-glutamylcyclotransferase 1 isoform a [Homo sapiens]

Protein Classification

glutathione-specific gamma-glutamylcyclotransferase( domain architecture ID 10520616)

glutathione-specific gamma-glutamylcyclotransferase (gamma-glu-GCG or gamma-GCG) catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide; acts specifically on glutathione, but not on other gamma-glutamyl peptides

CATH:  3.10.490.10
EC:  4.3.2.7
Gene Ontology:  GO:0016746
PubMed:  18515354|23070364
SCOP:  4002485

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 33-207 2.00e-98

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


:

Pssm-ID: 398428  Cd Length: 176  Bit Score: 283.51  E-value: 2.00e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  33 LWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLN 112
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111 113 VREAVlGGYDTKEVTFYP-QDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGP 191
Cdd:pfam04752  81 VREQI-NGYTTHEVPFYPdQDTDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGP 159

                         170
                  ....*....|....*.
gi 1316032111 192 QAQDEHLAAIVDAVGT 207
Cdd:pfam04752 160 GVQDDHLFELEKLVKK 175
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 33-207 2.00e-98

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 283.51  E-value: 2.00e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  33 LWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLN 112
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111 113 VREAVlGGYDTKEVTFYP-QDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGP 191
Cdd:pfam04752  81 VREQI-NGYTTHEVPFYPdQDTDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGP 159

                         170
                  ....*....|....*.
gi 1316032111 192 QAQDEHLAAIVDAVGT 207
Cdd:pfam04752 160 GVQDDHLFELEKLVKK 175
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 21-205 4.26e-56

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 176.55  E-value: 4.26e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  21 AQFPRNDGDPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLleDHEGCTWGVAYQVQ 100
Cdd:COG3703     4 NRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGL--DRGGSCRGVAFRVP 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111 101 GEQVSKALKYLNVREAVLGGYDTKEVTFypQDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLL 180
Cdd:COG3703    82 PEDAEAVLAYLWRREMVTGVYLPRWLPV--RLADGRRVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLL 159

                         170       180
                  ....*....|....*....|....*
gi 1316032111 181 RLADFMQLCGpqAQDEHLAAIVDAV 205
Cdd:COG3703   160 NTAEHLRELG--IRDPHLEALARLV 182
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 35-145 2.38e-16

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 71.61  E-value: 2.38e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  35 IFGYGSLVWRPDFA-----YSDSRVGFVRGYSRRFWQGdtfhrgsdkmpGRVVTLLEDHEGCTWGVAYQVqgeqVSKALK 109
Cdd:cd06661     1 LFVYGTLMDGEVLHarlgrALFLGPATLKGYRLVFGGG-----------SGYPGLVPGPGARVWGELYEV----DPEDLA 65

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1316032111 110 YLNVREAVLGGYDTKEVTFYPQDapDQPLKALAYVA 145
Cdd:cd06661    66 RLDAFEGVPGGYRREEVEVELED--GEGVEAWVYVA 99
 
Name Accession Description Interval E-value
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 33-207 2.00e-98

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 283.51  E-value: 2.00e-98
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  33 LWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLN 112
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDEEGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111 113 VREAVlGGYDTKEVTFYP-QDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGP 191
Cdd:pfam04752  81 VREQI-NGYTTHEVPFYPdQDTDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGP 159

                         170
                  ....*....|....*.
gi 1316032111 192 QAQDEHLAAIVDAVGT 207
Cdd:pfam04752 160 GVQDDHLFELEKLVKK 175
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 21-205 4.26e-56

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 176.55  E-value: 4.26e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  21 AQFPRNDGDPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLleDHEGCTWGVAYQVQ 100
Cdd:COG3703     4 NRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGL--DRGGSCRGVAFRVP 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111 101 GEQVSKALKYLNVREAVLGGYDTKEVTFypQDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLL 180
Cdd:COG3703    82 PEDAEAVLAYLWRREMVTGVYLPRWLPV--RLADGRRVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLL 159

                         170       180
                  ....*....|....*....|....*
gi 1316032111 181 RLADFMQLCGpqAQDEHLAAIVDAV 205
Cdd:COG3703   160 NTAEHLRELG--IRDPHLEALARLV 182
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 35-145 2.38e-16

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 71.61  E-value: 2.38e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1316032111  35 IFGYGSLVWRPDFA-----YSDSRVGFVRGYSRRFWQGdtfhrgsdkmpGRVVTLLEDHEGCTWGVAYQVqgeqVSKALK 109
Cdd:cd06661     1 LFVYGTLMDGEVLHarlgrALFLGPATLKGYRLVFGGG-----------SGYPGLVPGPGARVWGELYEV----DPEDLA 65

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1316032111 110 YLNVREAVLGGYDTKEVTFYPQDapDQPLKALAYVA 145
Cdd:cd06661    66 RLDAFEGVPGGYRREEVEVELED--GEGVEAWVYVA 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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