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Conserved domains on  [gi|21312862|ref|NP_080558|]
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kinetochore protein Spc24 isoform 1 [Mus musculus]

Protein Classification

kinetochore Spc24 family protein( domain architecture ID 10550629)

kinetochore Spc24 family protein acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity

Gene Ontology:  GO:0000776|GO:0051301

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
Spc24 pfam08286
Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved ...
 82-192 2.41e-25

Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation


:

Pssm-ID: 429899 [Multi-domain]  Cd Length: 107  Bit Score: 94.59  E-value: 2.41e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862    82 QLEVELQRTSKEDTCVQARLRQLITELQELREMEEELQRQERDVDEDNTVTIPSAVYVAHLYHQIsKIQWDYECEPGMIK 161
Cdd:pfam08286   1 ELDNEKFRLAKELNDLESELERLESELAKLKEELEELEEQGVEVDEEDERSEDETVLKLKLYRSL-GIELEYDYNKALIR 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 21312862   162 GIHHGptVAQPIHLDSaQLSPKFISDYLWSL 192
Cdd:pfam08286  80 NKDKG--DVHVVNLDP-KLSDFFYANYLWDL 107
SMC_prok_B super family cl37069
chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of ...
 15-130 6.46e-04

chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. This family represents the SMC protein of most bacteria. The smc gene is often associated with scpB (TIGR00281) and scpA genes, where scp stands for segregation and condensation protein. SMC was shown (in Caulobacter crescentus) to be induced early in S phase but present and bound to DNA throughout the cell cycle. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins]


The actual alignment was detected with superfamily member TIGR02168:

Pssm-ID: 274008 [Multi-domain]  Cd Length: 1179  Bit Score: 40.04  E-value: 6.46e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862     15 LSLLGANRAEAQQRRllgsyeQMMERLLEMQDGAYRQLRETLAVEEEVAQSL-LELKECTRQGDTELQQLEVELQRTSKE 93
Cdd:TIGR02168  812 LTLLNEEAANLRERL------ESLERRIAATERRLEDLEEQIEELSEDIESLaAEIEELEELIEELESELEALLNERASL 885

                           90       100       110
                   ....*....|....*....|....*....|....*..
gi 21312862     94 DTCVQARLRQLITELQELREMEEELQRQERDVDEDNT 130
Cdd:TIGR02168  886 EEALALLRSELEELSEELRELESKRSELRRELEELRE 922
 
Name Accession Description Interval E-value
Spc24 pfam08286
Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved ...
 82-192 2.41e-25

Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation


Pssm-ID: 429899 [Multi-domain]  Cd Length: 107  Bit Score: 94.59  E-value: 2.41e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862    82 QLEVELQRTSKEDTCVQARLRQLITELQELREMEEELQRQERDVDEDNTVTIPSAVYVAHLYHQIsKIQWDYECEPGMIK 161
Cdd:pfam08286   1 ELDNEKFRLAKELNDLESELERLESELAKLKEELEELEEQGVEVDEEDERSEDETVLKLKLYRSL-GIELEYDYNKALIR 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 21312862   162 GIHHGptVAQPIHLDSaQLSPKFISDYLWSL 192
Cdd:pfam08286  80 NKDKG--DVHVVNLDP-KLSDFFYANYLWDL 107
RWD_Spc24 cd11565
RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; ...
 136-193 6.50e-12

RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; The kinetochore protein Spc24 acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Spc24 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. The model corresponds to the Spc24 RWD domain, which provides an interaction platform to mediate kinetochore assembly.


Pssm-ID: 467842  Cd Length: 58  Bit Score: 58.29  E-value: 6.50e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862 136 AVYVAHLYHQIsKIQWDYECEPGMIKGIHHGPT--VAQPIHLDSaQLSPKFISDYLWSLV 193
Cdd:cd11565   1 TVLKLKLYRSL-GIELEEDEDGEYDKALIRNKNkgDVHVVNLDD-KYSDYFYSNYLWDLL 58
SMC_prok_B TIGR02168
chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of ...
 15-130 6.46e-04

chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. This family represents the SMC protein of most bacteria. The smc gene is often associated with scpB (TIGR00281) and scpA genes, where scp stands for segregation and condensation protein. SMC was shown (in Caulobacter crescentus) to be induced early in S phase but present and bound to DNA throughout the cell cycle. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins]


Pssm-ID: 274008 [Multi-domain]  Cd Length: 1179  Bit Score: 40.04  E-value: 6.46e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862     15 LSLLGANRAEAQQRRllgsyeQMMERLLEMQDGAYRQLRETLAVEEEVAQSL-LELKECTRQGDTELQQLEVELQRTSKE 93
Cdd:TIGR02168  812 LTLLNEEAANLRERL------ESLERRIAATERRLEDLEEQIEELSEDIESLaAEIEELEELIEELESELEALLNERASL 885

                           90       100       110
                   ....*....|....*....|....*....|....*..
gi 21312862     94 DTCVQARLRQLITELQELREMEEELQRQERDVDEDNT 130
Cdd:TIGR02168  886 EEALALLRSELEELSEELRELESKRSELRRELEELRE 922
Smc COG1196
Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning]; ...
 50-128 6.08e-03

Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440809 [Multi-domain]  Cd Length: 983  Bit Score: 36.84  E-value: 6.08e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 21312862  50 RQLRETLAVEEEVAQSLLELKECTRQGDTELQQLEVELQRTSKEDTCVQARLRQLITELQELREMEEELQRQERDVDED 128
Cdd:COG1196 239 AELEELEAELEELEAELEELEAELAELEAELEELRLELEELELELEEAQAEEYELLAELARLEQDIARLEERRRELEER 317
Macoilin pfam09726
Macoilin family; The Macoilin proteins has an N-terminal portion that is composed of 5 ...
 33-116 9.28e-03

Macoilin family; The Macoilin proteins has an N-terminal portion that is composed of 5 trasnmembrane helices, followed by a C-terminal coiled-coil region. Macoilin is a highly conserved protein present in eukaryotes. Macoilin appears to be found in the ER and be involved in the function of neurons.


Pssm-ID: 462859 [Multi-domain]  Cd Length: 670  Bit Score: 36.37  E-value: 9.28e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862    33 SYEQMMERLLEMQDGayRQLRETLAVEEE-------------VAQSLLELKECTRQGDTELQQLEVELQRTSKEDTCVQA 99
Cdd:pfam09726 466 TVQQLEKRLKAEQEA--RASAEKQLAEEKkrkkeeeataaraVALAAASRGECTESLKQRKRELESEIKKLTHDIKLKEE 543

                          90
                  ....*....|....*..
gi 21312862   100 RLRQLITELQELREMEE 116
Cdd:pfam09726 544 QIRELEIKVQELRKYKE 560
Smc COG1196
Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning]; ...
 23-128 9.34e-03

Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440809 [Multi-domain]  Cd Length: 983  Bit Score: 36.45  E-value: 9.34e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862  23 AEAQQRRLLGSYEQMMERLLEMQdGAYRQLRETLAVEEEVAQSLLELKectRQGDTELQQLEVELQRTSKEDTCVQARLR 102
Cdd:COG1196 265 LEAELEELRLELEELELELEEAQ-AEEYELLAELARLEQDIARLEERR---RELEERLEELEEELAELEEELEELEEELE 340

                        90       100       110
                ....*....|....*....|....*....|
gi 21312862 103 QLITELQ----ELREMEEELQRQERDVDED 128
Cdd:COG1196 341 ELEEELEeaeeELEEAEAELAEAEEALLEA 370
 
Name Accession Description Interval E-value
Spc24 pfam08286
Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved ...
 82-192 2.41e-25

Spc24 subunit of Ndc80; Spc24 is a component of the evolutionarily conserved kinetochore-associated Ndc80 complex and is involved in chromosome segregation


Pssm-ID: 429899 [Multi-domain]  Cd Length: 107  Bit Score: 94.59  E-value: 2.41e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862    82 QLEVELQRTSKEDTCVQARLRQLITELQELREMEEELQRQERDVDEDNTVTIPSAVYVAHLYHQIsKIQWDYECEPGMIK 161
Cdd:pfam08286   1 ELDNEKFRLAKELNDLESELERLESELAKLKEELEELEEQGVEVDEEDERSEDETVLKLKLYRSL-GIELEYDYNKALIR 79

                          90       100       110
                  ....*....|....*....|....*....|.
gi 21312862   162 GIHHGptVAQPIHLDSaQLSPKFISDYLWSL 192
Cdd:pfam08286  80 NKDKG--DVHVVNLDP-KLSDFFYANYLWDL 107
RWD_Spc24 cd11565
RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; ...
 136-193 6.50e-12

RWD domain of the Spc24 component of the nuclear division cycle 80 (NDC80) kinetochore complex; The kinetochore protein Spc24 acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Spc24 is required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. The model corresponds to the Spc24 RWD domain, which provides an interaction platform to mediate kinetochore assembly.


Pssm-ID: 467842  Cd Length: 58  Bit Score: 58.29  E-value: 6.50e-12
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862 136 AVYVAHLYHQIsKIQWDYECEPGMIKGIHHGPT--VAQPIHLDSaQLSPKFISDYLWSLV 193
Cdd:cd11565   1 TVLKLKLYRSL-GIELEEDEDGEYDKALIRNKNkgDVHVVNLDD-KYSDYFYSNYLWDLL 58
SMC_prok_B TIGR02168
chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of ...
 15-130 6.46e-04

chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. This family represents the SMC protein of most bacteria. The smc gene is often associated with scpB (TIGR00281) and scpA genes, where scp stands for segregation and condensation protein. SMC was shown (in Caulobacter crescentus) to be induced early in S phase but present and bound to DNA throughout the cell cycle. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins]


Pssm-ID: 274008 [Multi-domain]  Cd Length: 1179  Bit Score: 40.04  E-value: 6.46e-04
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862     15 LSLLGANRAEAQQRRllgsyeQMMERLLEMQDGAYRQLRETLAVEEEVAQSL-LELKECTRQGDTELQQLEVELQRTSKE 93
Cdd:TIGR02168  812 LTLLNEEAANLRERL------ESLERRIAATERRLEDLEEQIEELSEDIESLaAEIEELEELIEELESELEALLNERASL 885

                           90       100       110
                   ....*....|....*....|....*....|....*..
gi 21312862     94 DTCVQARLRQLITELQELREMEEELQRQERDVDEDNT 130
Cdd:TIGR02168  886 EEALALLRSELEELSEELRELESKRSELRRELEELRE 922
SMC_prok_B TIGR02168
chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of ...
 25-128 1.25e-03

chromosome segregation protein SMC, common bacterial type; SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. This family represents the SMC protein of most bacteria. The smc gene is often associated with scpB (TIGR00281) and scpA genes, where scp stands for segregation and condensation protein. SMC was shown (in Caulobacter crescentus) to be induced early in S phase but present and bound to DNA throughout the cell cycle. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins]


Pssm-ID: 274008 [Multi-domain]  Cd Length: 1179  Bit Score: 39.27  E-value: 1.25e-03
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862     25 AQQRRLLGSYEQMMERLLEMQDGAYRQLRETLAVEEEVAQSLLELKECTRQGDTELQQLEVELQRTSKEDTCVQARLRQL 104
Cdd:TIGR02168  750 AQLSKELTELEAEIEELEERLEEAEEELAEAEAEIEELEAQIEQLKEELKALREALDELRAELTLLNEEAANLRERLESL 829

                           90       100
                   ....*....|....*....|....
gi 21312862    105 ITELQELREMEEELQRQERDVDED 128
Cdd:TIGR02168  830 ERRIAATERRLEDLEEQIEELSED 853
Smc COG1196
Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning]; ...
 50-128 6.08e-03

Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440809 [Multi-domain]  Cd Length: 983  Bit Score: 36.84  E-value: 6.08e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 21312862  50 RQLRETLAVEEEVAQSLLELKECTRQGDTELQQLEVELQRTSKEDTCVQARLRQLITELQELREMEEELQRQERDVDED 128
Cdd:COG1196 239 AELEELEAELEELEAELEELEAELAELEAELEELRLELEELELELEEAQAEEYELLAELARLEQDIARLEERRRELEER 317
ClpA COG0542
ATP-dependent Clp protease, ATP-binding subunit ClpA [Posttranslational modification, protein ...
 68-121 7.58e-03

ATP-dependent Clp protease, ATP-binding subunit ClpA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440308 [Multi-domain]  Cd Length: 836  Bit Score: 36.60  E-value: 7.58e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 21312862  68 ELKECTRqgdtELQQLEVELQRTSKE-DTCVQARLRQLITELQELREMEEELQRQ 121
Cdd:COG0542 412 ELDELER----RLEQLEIEKEALKKEqDEASFERLAELRDELAELEEELEALKAR 462
Fez1 pfam06818
Fez1; This family represents the eukaryotic Fez1 protein. Fez1 contains a leucine-zipper ...
 51-113 9.09e-03

Fez1; This family represents the eukaryotic Fez1 protein. Fez1 contains a leucine-zipper region with similarity to the DNA-binding domain of the cAMP-responsive activating-transcription factor 5. There is evidence that Fez1 inhibits cancer cell growth through regulation of mitosis, and that its alterations result in abnormal cell growth. Note that some family members contain more than one copy of this region.


Pssm-ID: 462015 [Multi-domain]  Cd Length: 198  Bit Score: 35.74  E-value: 9.09e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 21312862    51 QLRETLAVEEEVAQSLLELKECTRQGDTELQQLEVELQRTSKEDTCVQARLRQLITELQELRE 113
Cdd:pfam06818  39 QLRELRAKLEEKEEQIQELEDSLRSKTLELEVCENELQRKKNEAELLREKVGKLEEEVSGLRE 101
Macoilin pfam09726
Macoilin family; The Macoilin proteins has an N-terminal portion that is composed of 5 ...
 33-116 9.28e-03

Macoilin family; The Macoilin proteins has an N-terminal portion that is composed of 5 trasnmembrane helices, followed by a C-terminal coiled-coil region. Macoilin is a highly conserved protein present in eukaryotes. Macoilin appears to be found in the ER and be involved in the function of neurons.


Pssm-ID: 462859 [Multi-domain]  Cd Length: 670  Bit Score: 36.37  E-value: 9.28e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862    33 SYEQMMERLLEMQDGayRQLRETLAVEEE-------------VAQSLLELKECTRQGDTELQQLEVELQRTSKEDTCVQA 99
Cdd:pfam09726 466 TVQQLEKRLKAEQEA--RASAEKQLAEEKkrkkeeeataaraVALAAASRGECTESLKQRKRELESEIKKLTHDIKLKEE 543

                          90
                  ....*....|....*..
gi 21312862   100 RLRQLITELQELREMEE 116
Cdd:pfam09726 544 QIRELEIKVQELRKYKE 560
Smc COG1196
Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning]; ...
 23-128 9.34e-03

Chromosome segregation ATPase Smc [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 440809 [Multi-domain]  Cd Length: 983  Bit Score: 36.45  E-value: 9.34e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312862  23 AEAQQRRLLGSYEQMMERLLEMQdGAYRQLRETLAVEEEVAQSLLELKectRQGDTELQQLEVELQRTSKEDTCVQARLR 102
Cdd:COG1196 265 LEAELEELRLELEELELELEEAQ-AEEYELLAELARLEQDIARLEERR---RELEERLEELEEELAELEEELEELEEELE 340

                        90       100       110
                ....*....|....*....|....*....|
gi 21312862 103 QLITELQ----ELREMEEELQRQERDVDED 128
Cdd:COG1196 341 ELEEELEeaeeELEEAEAELAEAEEALLEA 370
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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