LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 2 (ANKL2); The family includes ANKL2 and similar proteins. Although their biological roles remain unclear, the family members share an N-terminal LEM domain and an ankyrin repeat region. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The ankyrin repeats are unique motifs mediating protein-protein interactions.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 40789239  73 RLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQ 115
Cdd:cd12944   1 RLKKLSPDELREELRKAGLKCGPITSTTRFLFEKKLAQALLEQ 43

Caulimovirus viroplasmin; This family consists of various caulimovirus viroplasmin proteins. The viroplasmin protein is encoded by gene VI and is the main component of viral inclusion bodies or viroplasms. Inclusions are the site of viral assembly, DNA synthesis and accumulation. Two domains exist within gene VI corresponding approximately to the 5' third and middle third of gene VI, these influence systemic infection in a light-dependent manner.

                          10        20        30
                  ....*....|....*....|....*....|..
gi 40789239   225 VYEDKKEALQAAKLIKGSRFKAFRTREDAEKF 256
Cdd:pfam01693  13 IYTTWDECKAQVKGFPGAKYKSFKTREEAEAF 44

LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 2 (ANKL2); The family includes ANKL2 and similar proteins. Although their biological roles remain unclear, the family members share an N-terminal LEM domain and an ankyrin repeat region. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The ankyrin repeats are unique motifs mediating protein-protein interactions.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 40789239  73 RLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQ 115
Cdd:cd12944   1 RLKKLSPDELREELRKAGLKCGPITSTTRFLFEKKLAQALLEQ 43

LEM domain; The LEM domain is 50 residues long and is composed of two parallel alpha helices. This domain is found in inner nuclear membrane proteins. It is called the LEM domain after LAP2, Emerin and Man1.

                          10        20        30
                  ....*....|....*....|....*....|..
gi 40789239    77 LNPDDLRKEVMKAGLKCGPITSTTRFIFEKKL 108
Cdd:pfam03020   5 LSDEELREQLKEYGVSPGPITATTRKLYEKKL 36

Ankyrin repeats (many copies);

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 40789239   411 LNTPDKVGFdTPLHFACKFGNVDVVNVLSSHPLiVKNRKNKYGKTP 456
Cdd:pfam13857   9 LNRLDGEGY-TPLHVAAKYGALEIVRVLLAYGV-DLNLKDEEGLTA 52

in nuclear membrane-associated proteins; LEM, domain in nuclear membrane-associated proteins, including lamino-associated polypeptide 2 and emerin.

                           10        20        30
                   ....*....|....*....|....*....|..
gi 40789239     77 LNPDDLRKEVMKAGLKCGPITSTTRFIFEKKL 108
Cdd:smart00540   6 LSDAELRAELKQYGLPPGPITDTTRKLYEKKL 37

LEM (Lap2/Emerin/Man1) domain found in ankyrin repeat and LEM domain-containing protein 2 (ANKL2); The family includes ANKL2 and similar proteins. Although their biological roles remain unclear, the family members share an N-terminal LEM domain and an ankyrin repeat region. The LEM domain, mainly found in inner nuclear membrane proteins, may be involved in protein- or DNA-binding. The ankyrin repeats are unique motifs mediating protein-protein interactions.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 40789239  73 RLKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQALLEQ 115
Cdd:cd12944   1 RLKKLSPDELREELRKAGLKCGPITSTTRFLFEKKLAQALLEQ 43

LEM (Lap2/Emerin/Man1) domain found in lamina-associated polypeptide 2 (LAP2), LEM domain-containing protein 1 (LEMP-1) and similar proteins; This CD corresponds to the LEM domain of LAP2, LEMP-1 and similar proteins. LAP2, also termed thymopoietin (TP), or thymopoietin-related peptide (TPRP), is composed of isoform alpha and isoforms beta/gamma and may be involved in chromatin organization and post-mitotic reassembly. Some of LAP2 isoforms are inner nuclear membrane proteins that can bind to nuclear lamins and chromatin, while others are non-membrane nuclear polypeptides. All LAP2 isoforms contain an N-terminal LEM domain that is connected to a highly divergent LEM-like domain by an unstructured linker. Although LEM and LEM-like domains share the same structural fold composed of two large parallel alpha helices, the biochemical nature of the solvent-accessible residues is completely different, indicating that the two domains may target different protein surfaces. The LEM domain interacts with the nonspecific DNA binding protein barrier-to-autointegration factor (BAF) while the LEM-like domain is involved in chromosome binding. LEMP-1, also termed cancer/testis antigen 50 (CT50), is encoded by LEMD1, a novel testis-specific gene expressed in colorectal cancers. It may function as a cancer-testis antigen for immunotherapy of colorectal carcinoma (CRC). LEMP-1 contains an N-terminal LEM domain.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 40789239  74 LKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLaQALLEQ 115
Cdd:cd12940   2 VTELSDEELKAQLLKYGVKPGPITASTRKLYEKKL-QKLLDQ 42

LEM domain; The LEM domain is 50 residues long and is composed of two parallel alpha helices. This domain is found in inner nuclear membrane proteins. It is called the LEM domain after LAP2, Emerin and Man1.

                          10        20        30
                  ....*....|....*....|....*....|..
gi 40789239    77 LNPDDLRKEVMKAGLKCGPITSTTRFIFEKKL 108
Cdd:pfam03020   5 LSDEELREQLKEYGVSPGPITATTRKLYEKKL 36

LEM (Lap2/Emerin/Man1) domain found in LEM domain-containing protein 2 (LEM2); This CD corresponds to the LEM domain that is responsible for the interaction with chromatin protein barrier-to-autointegration factor (BAF). LEM2, also termed LEMD2, is a novel Man1-related ubiquitously expressed inner nuclear membrane protein required for normal nuclear envelope morphology. Association with lamin A is required for its proper nuclear envelope localization. It also binds to lamin C and plays an important role in the organization of lamin A/C complexes. LEM2 contains an N-terminal LEM domain, two putative transmembrane domains and a MAN1-Src1p C-terminal (MSC) domain, but lacks the Man1-specific C-terminal RNA recognition motif (RRM).

                        10        20        30
                ....*....|....*....|....*....|..
gi 40789239  77 LNPDDLRKEVMKAGLKCGPITSTTRFIFEKKL 108
Cdd:cd12941   1 LTDAELRRELLALGFRPGPITETTRKVYIKKL 32

Caulimovirus viroplasmin; This family consists of various caulimovirus viroplasmin proteins. The viroplasmin protein is encoded by gene VI and is the main component of viral inclusion bodies or viroplasms. Inclusions are the site of viral assembly, DNA synthesis and accumulation. Two domains exist within gene VI corresponding approximately to the 5' third and middle third of gene VI, these influence systemic infection in a light-dependent manner.

                          10        20        30
                  ....*....|....*....|....*....|..
gi 40789239   225 VYEDKKEALQAAKLIKGSRFKAFRTREDAEKF 256
Cdd:pfam01693  13 IYTTWDECKAQVKGFPGAKYKSFKTREEAEAF 44

LEM (Lap2/Emerin/Man1) domain found in emerin; This CD corresponds to the LEM domain that is critical for binding to lamin A/C and is also involved in interaction with the DNA binding protein barrier-to-autointegration factor (BAF). Emerin is an inner nuclear membrane protein that occurs in four differently phosphorylated forms and plays a role in cell cycle-dependent events. It is absent from the inner nuclear membrane in most patients with X-linked muscular dystrophy. Emerin interacts with A-type and B-type lamins. It contains an N-terminal LEM domain followed by a poly-serine segment, a region rich in hydrophobic amino acids comprising the nuclear localization signal (NLS) followed by another poly-serine segment, and a C-terminal transmembrane region.

                        10        20        30
                ....*....|....*....|....*....|....*...
gi 40789239  74 LKLLNPDDLRKEVMKAGLKCGPITSTTRFIFEKKLAQA 111
Cdd:cd12939   1 YKDLSDDELIKVLRKYGIKHGPVVGSTRKLYEKKLREA 38