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Conserved domains on  [gi|15217513|ref|NP_177304|]
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Nuclear transport factor 2 (NTF2) family protein [Arabidopsis thaliana]

Protein Classification

ketosteroid isomerase family protein( domain architecture ID 10489471)

ketosteroid isomerase family protein belongs to the nuclear transport factor 2 (NTF2-like) superfamily, similar to Mycobacterium tuberculosis Rv0760c, a structural homolog of delta(5)-3-ketosteroid isomerase

CATH:  3.10.450.50
SCOP:  3000472

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 71-192 3.18e-21

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


:

Pssm-ID: 396625  Cd Length: 116  Bit Score: 84.71  E-value: 3.18e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513    71 ASEVVSSFYAAVNVHDLSSVTDLIAQDCVYEDLVFSSPFVGRKAILDFFGKFIESTStdlQFVIDDI-STEDSSAVGVSW 149
Cdd:pfam02136   2 GNAFVQQYYAALDAHDPEGLHALYADDASMLTPPGSDPVVGLEAINEFFDSLPFTRI---QHKITSVdSQPASLKDGVLV 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 15217513   150 HLEWKGKNFPFsKGCSFYRLEVIDGKRqivyGRDCVEPAIKPG 192
Cdd:pfam02136  79 MVTGELTVDDF-PRRRFSQTFLLAPQR----GGYFVLNDIFRY 116
 
Name Accession Description Interval E-value
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 71-192 3.18e-21

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


Pssm-ID: 396625  Cd Length: 116  Bit Score: 84.71  E-value: 3.18e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513    71 ASEVVSSFYAAVNVHDLSSVTDLIAQDCVYEDLVFSSPFVGRKAILDFFGKFIESTStdlQFVIDDI-STEDSSAVGVSW 149
Cdd:pfam02136   2 GNAFVQQYYAALDAHDPEGLHALYADDASMLTPPGSDPVVGLEAINEFFDSLPFTRI---QHKITSVdSQPASLKDGVLV 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 15217513   150 HLEWKGKNFPFsKGCSFYRLEVIDGKRqivyGRDCVEPAIKPG 192
Cdd:pfam02136  79 MVTGELTVDDF-PRRRFSQTFLLAPQR----GGYFVLNDIFRY 116
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
70-188 3.16e-14

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 66.49  E-value: 3.16e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  70 SASEVVSSFYAAVNVHDLSSVTDLIAQDCVYEDLV--FSSPFVGRKAILDFFGKFIESTsTDLQFVIDDISTEDSSAVgV 147
Cdd:COG3631   1 TNRELVRRFYAAFNAGDVDALLALLAEDVVWEDPGgpGAGTYRGKEAVRAFFARLAAAF-EDLRFEVRRVVADGDRVV-V 78

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 15217513 148 SWHLEWK----GKNFPFSkGCSFYRLEviDGKrqIVYGRDCVEPA 188
Cdd:COG3631  79 EGTVRGTvlatGKPYENR-YADVFTVR--DGK--IVRVREYFDTA 118
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 74-173 6.40e-07

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 46.65  E-value: 6.40e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  74 VVSSFYAAVNVHDLSSVTDLIAQDCVYEDLVFSSPFVGRKAILDFFGKFIEST--STDLQFVidDISTEDSSAVGVSWHL 151
Cdd:cd00781   8 AVQRYVEAVNAGDPEGIVALFADDATVEDPVGSPPRSGRAAIAAFYAQSLGGAkrLELTGPV--RASHGGEAAFAFRVEF 85

                        90       100
                ....*....|....*....|..
gi 15217513 152 EWKGKNfpfskgcsfYRLEVID 173
Cdd:cd00781  86 EWEGQP---------CVVRVID 98
 
Name Accession Description Interval E-value
NTF2 pfam02136
Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like ...
 71-192 3.18e-21

Nuclear transport factor 2 (NTF2) domain; This family includes the NTF2-like Delta-5-3-ketosteroid isomerase proteins.


Pssm-ID: 396625  Cd Length: 116  Bit Score: 84.71  E-value: 3.18e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513    71 ASEVVSSFYAAVNVHDLSSVTDLIAQDCVYEDLVFSSPFVGRKAILDFFGKFIESTStdlQFVIDDI-STEDSSAVGVSW 149
Cdd:pfam02136   2 GNAFVQQYYAALDAHDPEGLHALYADDASMLTPPGSDPVVGLEAINEFFDSLPFTRI---QHKITSVdSQPASLKDGVLV 78

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 15217513   150 HLEWKGKNFPFsKGCSFYRLEVIDGKRqivyGRDCVEPAIKPG 192
Cdd:pfam02136  79 MVTGELTVDDF-PRRRFSQTFLLAPQR----GGYFVLNDIFRY 116
YesE COG3631
Ketosteroid isomerase-related protein [General function prediction only];
70-188 3.16e-14

Ketosteroid isomerase-related protein [General function prediction only];


Pssm-ID: 442849  Cd Length: 126  Bit Score: 66.49  E-value: 3.16e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  70 SASEVVSSFYAAVNVHDLSSVTDLIAQDCVYEDLV--FSSPFVGRKAILDFFGKFIESTsTDLQFVIDDISTEDSSAVgV 147
Cdd:COG3631   1 TNRELVRRFYAAFNAGDVDALLALLAEDVVWEDPGgpGAGTYRGKEAVRAFFARLAAAF-EDLRFEVRRVVADGDRVV-V 78

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 15217513 148 SWHLEWK----GKNFPFSkGCSFYRLEviDGKrqIVYGRDCVEPA 188
Cdd:COG3631  79 EGTVRGTvlatGKPYENR-YADVFTVR--DGK--IVRVREYFDTA 118
SnoaL_2 pfam12680
SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.
 75-175 1.74e-13

SnoaL-like domain; This family contains a large number of proteins that share the SnoaL fold.


Pssm-ID: 432716 [Multi-domain]  Cd Length: 100  Bit Score: 63.84  E-value: 1.74e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513    75 VSSFYAAVNVHDLSSVTDLIAQDCVYEDLVfsSPFVGRKAILDFFGKFIEsTSTDLQFVIDDISTEDSSAVgVSWHLE-- 152
Cdd:pfam12680   1 VRRFYEALNAGDLDALAALFAPDAVFHDPG--GPLRGRDAIRAFFAALFA-AFPDLRFEIHDVIADGDRVA-VRWTVTgt 76

                          90       100
                  ....*....|....*....|....*
gi 15217513   153 --WKGKNFPFsKGCSFYRLEviDGK 175
Cdd:pfam12680  77 ipPTGRGVRV-RGVDVFRFR--DGK 98
YybH COG4319
Ketosteroid isomerase homolog YybH [General function prediction only];
73-152 6.39e-07

Ketosteroid isomerase homolog YybH [General function prediction only];


Pssm-ID: 443460 [Multi-domain]  Cd Length: 131  Bit Score: 47.06  E-value: 6.39e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  73 EVVSSFYAAVNVHDLSSVTDLIAQDCVYEDlVFSSPFVGRKAILDFFGKFIEsTSTDLQFVIDDISTEDSSAVGVSWHLE 152
Cdd:COG4319  13 ALLAAFAEAFNAGDADALAALYAEDAVFFD-PGGPPVRGREAIRAAWAAAFA-AGPRVTFEVEDVRVLVSGDVAVVTGRW 90
ketosteroid_isomerase cd00781
ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond ...
 74-173 6.40e-07

ketosteroid isomerase: Many biological reactions proceed by enzymatic cleavage of a C-H bond adjacent to carbonyl or a carboxyl group, leading to an enol or a enolate intermediate that is subsequently re-protonated at the same or an adjacent carbon. Ketosteroid isomerases are important members of this class of enzymes which are the most proficient of all enzymes known and have served as a paradigm for enzymatic enolizations since its discovery in 1954. This CD includes members of this class that calalyze the isomerization of various beta,gamma-unsaturated isomers at nearly a diffusion-controlled rate. These enzymes are widely distributed in bacteria.


Pssm-ID: 238404  Cd Length: 122  Bit Score: 46.65  E-value: 6.40e-07
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  74 VVSSFYAAVNVHDLSSVTDLIAQDCVYEDLVFSSPFVGRKAILDFFGKFIEST--STDLQFVidDISTEDSSAVGVSWHL 151
Cdd:cd00781   8 AVQRYVEAVNAGDPEGIVALFADDATVEDPVGSPPRSGRAAIAAFYAQSLGGAkrLELTGPV--RASHGGEAAFAFRVEF 85

                        90       100
                ....*....|....*....|..
gi 15217513 152 EWKGKNfpfskgcsfYRLEVID 173
Cdd:cd00781  86 EWEGQP---------CVVRVID 98
NTF2_like cd00531
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 71-179 6.61e-06

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


Pssm-ID: 238296 [Multi-domain]  Cd Length: 124  Bit Score: 44.04  E-value: 6.61e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  71 ASEVVSSFYAAVNVHDLSSVTDLIAQDCVYEDLVFSS---PFVGRKAILDFFGKFIESTSTDLQFV----IDDISTEDSS 143
Cdd:cd00531   1 AEQFLYRYARLLDAGDREWLALLYADDAYFEPPGGDGliyPDDGREAIEDRVRRLPFGPSRTRHLVsnvdVQPGDDGEGV 80

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 15217513 144 AVGVSWHLEWKGKNF-PFSKGCSFYRLEVIDGKRQIV 179
Cdd:cd00531  81 VVSVFGVLRTRGDGEqDVFAGGQTFVLRPQGGGGKIA 117
SnoaL pfam07366
SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in ...
 78-185 6.77e-06

SnoaL-like polyketide cyclase; This family includes SnoaL a polyketide cyclase involved in nogalamycin biosynthesis. This family was formerly known as DUF1486. The proteins in this family adopt a distorted alpha-beta barrel fold. Structural data together with site-directed mutagenesis experiments have shown that SnoaL has a different mechanism to that of the classical aldolase for catalysing intramolecular aldol condensation.


Pssm-ID: 399975  Cd Length: 126  Bit Score: 43.83  E-value: 6.77e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513    78 FYAAV-NVHDLSSVTDLIAQDCVYEDLVFSSPfVGRKAILDFFGKFIESTStDLQFVIDDISTEDSSAVgVSWHLEW--- 153
Cdd:pfam07366   6 FYDEVwNTGDLDALDEFVAEDVVDHNPPPGVA-RGLDGFRAFAEMLFRAFP-DLRFEVEDLVAEGDKVA-ARLTFTGtht 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 15217513   154 --------KGKNFPFSkGCSFYRleVIDGKrqIVYGRDCV 185
Cdd:pfam07366  83 geflgippTGRKVRFR-GIDLYR--FEDGK--IVEHWDVI 117
COG5485 COG5485
Predicted ester cyclase [General function prediction only];
74-175 4.79e-05

Predicted ester cyclase [General function prediction only];


Pssm-ID: 444236 [Multi-domain]  Cd Length: 130  Bit Score: 41.77  E-value: 4.79e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15217513  74 VVSSFYAAV-NVHDLSSVTDLIAQDCVYEDLVFSSPfVGRKAILDFFGKFIESTStDLQFVIDDISTEDsSAVGVSWHL- 151
Cdd:COG5485   1 LVRRFFEEAwNEGDLDALDELVAPDFVYHGPLPGEP-RGPEGFRAFLRGLRAAFP-DLRFEIEDLIAEG-DRVAVRWTFt 77

                        90       100       110
                ....*....|....*....|....*....|....
gi 15217513 152 -----EW-----KGKNFPFSkGCSFYRLEviDGK 175
Cdd:COG5485  78 gthtgEFlgippTGKRVEFR-GIDIYRFE--DGK 108
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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