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Conserved domains on  [gi|15218535|ref|NP_177405|]
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germin-like protein 1 [Arabidopsis thaliana]

Protein Classification

germin family protein( domain architecture ID 14388826)

germin family protein is a cupin domain-containing protein similar to oxalate oxidase (also called germin) that catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide

CATH:  2.60.120.10
Gene Ontology:  GO:0046872
PubMed:  14697267|19478949
SCOP:  3001825

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 19-204 2.28e-78

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


:

Pssm-ID: 380368  Cd Length: 191  Bit Score: 232.87  E-value: 2.28e-78
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  19 VQDFCVANLKRAETPAGYPCIRPIHVKATDFVFSGLGTPGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIP 98
Cdd:cd02241   5 LQDFCVADLSSPRTPNGYPCKDPALVTADDFVFDFLNPPGNTSNPLGGSVTLANVANFPALNGLGISMARGDLAPCGVNP 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  99 MHTHPGASEVLFVLTGSITAGFVSS-ANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALF 177
Cdd:cd02241  85 PHTHPRATELLYVVEGTLYVGFVDEnGNRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGTQQIAQALF 164

                       170       180
                ....*....|....*....|....*..
gi 15218535 178 ANSLPTELVVGTTFLDATTVKKLKGVL 204
Cdd:cd02241 165 GLPPPDDVLAAAFGLDGAQVEKLKSKF 191
 
Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 19-204 2.28e-78

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380368  Cd Length: 191  Bit Score: 232.87  E-value: 2.28e-78
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  19 VQDFCVANLKRAETPAGYPCIRPIHVKATDFVFSGLGTPGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIP 98
Cdd:cd02241   5 LQDFCVADLSSPRTPNGYPCKDPALVTADDFVFDFLNPPGNTSNPLGGSVTLANVANFPALNGLGISMARGDLAPCGVNP 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  99 MHTHPGASEVLFVLTGSITAGFVSS-ANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALF 177
Cdd:cd02241  85 PHTHPRATELLYVVEGTLYVGFVDEnGNRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGTQQIAQALF 164

                       170       180
                ....*....|....*....|....*..
gi 15218535 178 ANSLPTELVVGTTFLDATTVKKLKGVL 204
Cdd:cd02241 165 GLPPPDDVLAAAFGLDGAQVEKLKSKF 191
Cupin_1 pfam00190
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 52-198 2.91e-44

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 395138  Cd Length: 151  Bit Score: 144.78  E-value: 2.91e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535    52 SGLGTPGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFVSSANA--VYV 129
Cdd:pfam00190   1 LNLLEPGPTYNPEGGRVTTVNSKNLPGLNTLGISAARVDLAPGGMNPPHWHPNATEILYVLQGRGRVGFVVPGNGnrVFH 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15218535   130 QTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALFA--NSLPTELVVGTTFLDATTVK 198
Cdd:pfam00190  81 KVLREGDVFVVPQGLPHFQYNIGDEPAVAFVAFDTNNPGNQSILAGGFSslPALPPEVLAKAFQLAGEEVK 151
Cupin_1 smart00835
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 57-198 2.45e-39

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 214845 [Multi-domain]  Cd Length: 146  Bit Score: 132.02  E-value: 2.45e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535     57 PGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFV-SSANAVYVQTLKPG 135
Cdd:smart00835   3 PRPDFSNEGGRLREADPTNFPALNGLGISAARVNLEPGGMLPPHYHPRATELLYVVRGEGRVGVVdPNGNKVYDARLREG 82

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15218535    136 QVMVFPQGLLHFQINAGKSSAsAVVTFNSANPGLQILDFALFA--NSLPTELVVGTTFLDATTVK 198
Cdd:smart00835  83 DVFVVPQGHPHFQVNSGDENL-EFVAFNTNDPNRRFFLAGRNSvlRGLPPEVLAAAFGVSAEEVR 146
QdoI COG1917
Cupin domain protein related to quercetin dioxygenase [General function prediction only];
84-163 2.32e-09

Cupin domain protein related to quercetin dioxygenase [General function prediction only];


Pssm-ID: 441521 [Multi-domain]  Cd Length: 99  Bit Score: 52.54  E-value: 2.32e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  84 LSTARLDLAPKGVIPMHTHPGaSEVLFVLTGSITagfVSSANAVYvqTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFN 163
Cdd:COG1917  23 LEVVRVTFEPGARTPWHSHPG-EELIYVLEGEGE---VEVGGEEY--ELKPGDVVFIPPGVPHAFRNLGDEPAVLLVVFS 96
bicupin_oxalic TIGR03404
bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins ...
 56-201 7.19e-03

bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins as they have two copies of the cupin domain (pfam00190). Two different known activities for members of this family are oxalate decarboxylase (EC 4.1.1.2) and oxalate oxidase (EC 1.2.3.4), although the latter activity has more often been found in distantly related monocupin (germin) proteins.


Pssm-ID: 274565 [Multi-domain]  Cd Length: 367  Bit Score: 36.53  E-value: 7.19e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535    56 TPGNTTNIINAAVTP---AFAAqfpglnglglstARLDLAPKGVIPMHTHPGASEVLFVLTGSITAG-FVSSANAvyvQT 131
Cdd:TIGR03404 226 VPGGTVRIADSTNFPvskTIAA------------AIVTVEPGAMRELHWHPNADEWQYFIQGQARMTvFAAGGNA---RT 290

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15218535   132 L--KPGQVMVFPQGLLHFQINAGKSSASAVVTFNSanPGLQILDFALFANSLPTELVVGTTFLDATTVKKLK 201
Cdd:TIGR03404 291 FdyQAGDVGYVPRNMGHYVENTGDETLVFLEVFKA--DRFADVSLNQWLALTPPQLVAAHLNLDDEVIDSLK 360
 
Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 19-204 2.28e-78

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380368  Cd Length: 191  Bit Score: 232.87  E-value: 2.28e-78
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  19 VQDFCVANLKRAETPAGYPCIRPIHVKATDFVFSGLGTPGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIP 98
Cdd:cd02241   5 LQDFCVADLSSPRTPNGYPCKDPALVTADDFVFDFLNPPGNTSNPLGGSVTLANVANFPALNGLGISMARGDLAPCGVNP 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  99 MHTHPGASEVLFVLTGSITAGFVSS-ANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALF 177
Cdd:cd02241  85 PHTHPRATELLYVVEGTLYVGFVDEnGNRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGTQQIAQALF 164

                       170       180
                ....*....|....*....|....*..
gi 15218535 178 ANSLPTELVVGTTFLDATTVKKLKGVL 204
Cdd:cd02241 165 GLPPPDDVLAAAFGLDGAQVEKLKSKF 191
Cupin_1 pfam00190
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 52-198 2.91e-44

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 395138  Cd Length: 151  Bit Score: 144.78  E-value: 2.91e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535    52 SGLGTPGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFVSSANA--VYV 129
Cdd:pfam00190   1 LNLLEPGPTYNPEGGRVTTVNSKNLPGLNTLGISAARVDLAPGGMNPPHWHPNATEILYVLQGRGRVGFVVPGNGnrVFH 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15218535   130 QTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGLQILDFALFA--NSLPTELVVGTTFLDATTVK 198
Cdd:pfam00190  81 KVLREGDVFVVPQGLPHFQYNIGDEPAVAFVAFDTNNPGNQSILAGGFSslPALPPEVLAKAFQLAGEEVK 151
Cupin_1 smart00835
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 57-198 2.45e-39

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 214845 [Multi-domain]  Cd Length: 146  Bit Score: 132.02  E-value: 2.45e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535     57 PGNTTNIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFV-SSANAVYVQTLKPG 135
Cdd:smart00835   3 PRPDFSNEGGRLREADPTNFPALNGLGISAARVNLEPGGMLPPHYHPRATELLYVVRGEGRVGVVdPNGNKVYDARLREG 82

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15218535    136 QVMVFPQGLLHFQINAGKSSAsAVVTFNSANPGLQILDFALFA--NSLPTELVVGTTFLDATTVK 198
Cdd:smart00835  83 DVFVVPQGHPHFQVNSGDENL-EFVAFNTNDPNRRFFLAGRNSvlRGLPPEVLAAAFGVSAEEVR 146
cupin_OxDC-like cd20306
Oxalate decarboxylase (OxDC)-like cupin domain; This subfamily contains bacterial and ...
 42-191 5.76e-19

Oxalate decarboxylase (OxDC)-like cupin domain; This subfamily contains bacterial and eukaryotic cupin domains of proteins homologous to oxalate decarboxylase (OxDC; EC 4.1.1.2) such as MSMEG_2254, a putative OxDC from Mycobacterium smegmatis. OxDC is a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds with each domain containing one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues.


Pssm-ID: 380440 [Multi-domain]  Cd Length: 151  Bit Score: 79.56  E-value: 5.76e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  42 IHVKATDFVFSglgTPGNTtniinaaVTPAFAAQFPGLNGLGLstARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFV 121
Cdd:cd20306   4 FSLEDSNPFFE---SEGGS-------IRQATADQLPVLKGLSI--YRLRLSPGGIREPHWHPNANELGYVISGEARVSIL 71

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535 122 SSANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPglQILDFALFANSLPTElVVGTTF 191
Cdd:cd20306  72 DPTGSLDTFTVKPGQVVFIPQGWLHWIENVGDEEAHLLIFFNHETP--EDIGLSDSLRATPPE-VLGNTY 138
cupin_OxDC_C cd20305
Oxalate decarboxylase (OxDC), C-terminal cupin domain; This model represents the C-terminal ...
 84-201 4.37e-11

Oxalate decarboxylase (OxDC), C-terminal cupin domain; This model represents the C-terminal cupin domain of oxalate decarboxylase (OxDC; EC 4.1.1.2), a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds with each domain containing one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues. Members of this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380439 [Multi-domain]  Cd Length: 153  Bit Score: 58.75  E-value: 4.37e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  84 LSTARLDLAPKGVIPMHTHPGASEVLFVLTGS--ITAgFVSSANAVyVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVT 161
Cdd:cd20305  34 IAAALVTLEPGALRELHWHPNADEWQYYISGKarMTV-FASGGRAR-TFDFQAGDVGYVPRGYGHYIENTGDEPLEFLEV 111

                        90       100       110       120
                ....*....|....*....|....*....|....*....|
gi 15218535 162 FNSanPGLQILDFALFANSLPTELVVGTTFLDATTVKKLK 201
Cdd:cd20305 112 FNS--GRYQDISLSQWLALTPPDLVAAHLGLPDDTIAKLP 149
cupin_OxDC cd02240
Oxalate decarboxylase (OxDC), cupin domain; Oxalate decarboxylase (OxDC; EC 4.1.1.2) is a ...
 59-191 3.39e-10

Oxalate decarboxylase (OxDC), cupin domain; Oxalate decarboxylase (OxDC; EC 4.1.1.2) is a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds and both domains are included in this alignment. Each OxDC cupin domain contains one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues. Members of this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380367 [Multi-domain]  Cd Length: 145  Bit Score: 55.95  E-value: 3.39e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  59 NTTNIINAAVTPAFAAQ---FPGLNGLglSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFVSSANAVYVQTLKPG 135
Cdd:cd02240   1 DSEPIEENAGGSVRIATvtnFPISKDL--SSALVRVAPGAMRELHWHPNTAEWQYVISGSARVTVFDEDGRFETFNLGAG 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 15218535 136 QVMVFPQGLLHFQINAGKSSASAVVTFNsaNPGLQILDFALFANSLPTElVVGTTF 191
Cdd:cd02240  79 DVGYVPSGSGHHIENIGDEDAEFLLIFD--DGTFADVSLPWWLAMTPEE-VLAATL 131
QdoI COG1917
Cupin domain protein related to quercetin dioxygenase [General function prediction only];
84-163 2.32e-09

Cupin domain protein related to quercetin dioxygenase [General function prediction only];


Pssm-ID: 441521 [Multi-domain]  Cd Length: 99  Bit Score: 52.54  E-value: 2.32e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  84 LSTARLDLAPKGVIPMHTHPGaSEVLFVLTGSITagfVSSANAVYvqTLKPGQVMVFPQGLLHFQINAGKSSASAVVTFN 163
Cdd:COG1917  23 LEVVRVTFEPGARTPWHSHPG-EELIYVLEGEGE---VEVGGEEY--ELKPGDVVFIPPGVPHAFRNLGDEPAVLLVVFS 96
Cupin_2 pfam07883
Cupin domain; This family represents the conserved barrel domain of the 'cupin' superfamily ( ...
 87-162 3.10e-09

Cupin domain; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel).


Pssm-ID: 462300 [Multi-domain]  Cd Length: 71  Bit Score: 51.49  E-value: 3.10e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535    87 ARLDLAPKGVIPMHTHPGASEVLFVLTGSITagfvssanaVYV----QTLKPGQVMVFPQGLLHFQINAGKSSASAVVTF 162
Cdd:pfam07883   1 GLVTLPPGESSPPHRHPGEDEFFYVLEGEGE---------LTVdgeeVVLKAGDSVYFPAGVPHRFRNTGDEPARLLDVY 71
cupin_BLL4011-like cd02235
Bradyrhizobium diazoefficiens BLL4011 and related proteins, cupin domain; This family includes ...
 86-162 1.85e-08

Bradyrhizobium diazoefficiens BLL4011 and related proteins, cupin domain; This family includes bacterial and fungal proteins homologous to BLL4011, a Bradyrhizobium diazoefficiens protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380363 [Multi-domain]  Cd Length: 100  Bit Score: 50.27  E-value: 1.85e-08
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15218535  86 TARLDLAPKGVIPMHTHPGaSEVLFVLTGSITAGFVSSANavyvQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTF 162
Cdd:cd02235  21 QVRVEIPPGAVAGRHTHPG-EESGYVLEGSLELEVDGQPP----VTLKAGDSFFIPAGTVHNAKNVGSGPAKLLATY 92
ManC COG0662
Mannose-6-phosphate isomerase, cupin superfamily [Carbohydrate transport and metabolism];
62-153 2.14e-08

Mannose-6-phosphate isomerase, cupin superfamily [Carbohydrate transport and metabolism];


Pssm-ID: 440426 [Multi-domain]  Cd Length: 114  Bit Score: 50.53  E-value: 2.14e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  62 NIINAAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITagfvssanaVYV----QTLKPGQV 137
Cdd:COG0662   5 NIEELKAIGWGSYEVLGEGGERLSVKRITVPPGAELSLHVHPHRDEFFYVLEGTGE---------VTIgdeeVELKAGDS 75

                        90
                ....*....|....*.
gi 15218535 138 MVFPQGLLHFQINAGK 153
Cdd:COG0662  76 VYIPAGVPHRLRNPGD 91
OxdD COG2140
Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate ...
 83-167 5.51e-08

Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate transport and metabolism]; Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 441743 [Multi-domain]  Cd Length: 115  Bit Score: 49.19  E-value: 5.51e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  83 GLSTARLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFVSSANAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTF 162
Cdd:COG2140   2 TLAGGLTVLEPGGVREEHWHPNAAEWYYVLSGEARMTVQDPPGRARTVDVGPGDVVYVPPGYGHYIINTGDEPLVFLAVF 81


                ....*
gi 15218535 163 NSANP 167
Cdd:COG2140  82 DDDAG 86
cupin_RmlC-like cd02208
RmlC-like cupin superfamily; This superfamily contains proteins similar to the RmlC (dTDP ...
 88-162 6.36e-06

RmlC-like cupin superfamily; This superfamily contains proteins similar to the RmlC (dTDP (deoxythymidine diphosphates)-4-dehydrorhamnose 3,5-epimerase)-like cupins. RmlC is a dTDP-sugar isomerase involved in the synthesis of L-rhamnose, a saccharide required for the virulence of some pathogenic bacteria. Cupins are a functionally diverse superfamily originally discovered based on the highly conserved motif found in germin and germin-like proteins. This conserved motif forms a beta-barrel fold found in all of the cupins, giving rise to the name cupin ('cupa' is the Latin term for small barrel). The active site of members of this superfamily is generally located at the center of a conserved barrel and usually includes a metal ion. The different functional classes in this superfamily include single domain bacterial isomerases and epimerases involved in the modification of cell wall carbohydrates, two domain bicupins such as the desiccation-tolerant seed storage globulins, and multidomain nuclear transcription factors involved in legume root nodulation.


Pssm-ID: 380338 [Multi-domain]  Cd Length: 73  Bit Score: 42.86  E-value: 6.36e-06
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15218535  88 RLDLAPKGVIPMHTHPGASEVLFVLTGSITAGFvssaNAVYVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTF 162
Cdd:cd02208   3 VVTLPPGTSSPPHWHPEQDEIFYVLSGEGELTL----DDGETVELKAGDIVLIPPGVPHSFVNTSDEPAVFLVVS 73
cupin_MAE_RS03005 cd06987
Microcystis aeruginosa MAE_RS03005 and related proteins, cupin domain; This family includes ...
 93-154 6.92e-05

Microcystis aeruginosa MAE_RS03005 and related proteins, cupin domain; This family includes bacterial and some eukaryotic proteins homologous to MAE_RS03005, a Microcystis aeruginosa protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380392 [Multi-domain]  Cd Length: 122  Bit Score: 41.10  E-value: 6.92e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15218535  93 PKGVIPMHTHPGASEVLFVLTGSITAgfVSSANAVyvqTLKPGQVMVFPQGLLHFQINAGKS 154
Cdd:cd06987  37 PGGRTPPNTHPAAHEMFFVLAGEGRA--YCDGQRV---PLRPGDALVVPPGSEHVIENTGSG 93
cupin_CV2614-like cd02236
Chromobacterium violaceum CV2614 and related proteins, cupin domain; This family includes ...
 84-162 1.61e-04

Chromobacterium violaceum CV2614 and related proteins, cupin domain; This family includes mostly bacterial proteins homologous to CV2614, a Chromobacterium violaceum protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380364 [Multi-domain]  Cd Length: 102  Bit Score: 39.40  E-value: 1.61e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15218535  84 LSTARLDLAPKGVIPMHTHPgASEVLFVLTGSITagfVSSANAVyVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVTF 162
Cdd:cd02236  22 ITVLRITIPPGAELPWHTHP-VPNAGYVLSGELT---VEYEDGK-KRTFKAGDAFVEAVNTWHRGRNGGDEPVELLVFY 95
cupin_BLR7677-like cd02234
Bradyrhizobium japonicum BLR7677 and related proteins, cupin domain; This family includes ...
 66-162 8.24e-04

Bradyrhizobium japonicum BLR7677 and related proteins, cupin domain; This family includes bacterial and fungal proteins homologous to BLR7677, a Bradyrhizobium japonicum protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380362 [Multi-domain]  Cd Length: 103  Bit Score: 37.48  E-value: 8.24e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  66 AAVTPAFAAQFPGLNGLGLSTARLDLAPKGVIPMHTHPGaSEVLFVLTGSITAGFVSSAnavyVQTLKPGQVMVFPQGLL 145
Cdd:cd02234   1 ETVTVLYSQPLPNIPGKEVTVLLVTYPPGAASPPHRHPG-FVFAYVLEGEVRSQVNGGP----PRVYKAGESFYEPPGAH 75

                        90
                ....*....|....*....
gi 15218535 146 HFQI-NAGKS-SASAVVTF 162
Cdd:cd02234  76 HRVSrNASATePAKLLAVF 94
cupin_7S_vicilin-like_N cd02244
7S vicilin seed storage globulin, N-terminal cupin domain; This family contains the N-terminal ...
 96-168 1.07e-03

7S vicilin seed storage globulin, N-terminal cupin domain; This family contains the N-terminal domains of plant 7S seed storage proteins such as vicilin, and includes beta-conglycinin, phaseolin, canavalin, conglutin-beta, a chromatin protein in Pisum sativum called P54, and a sucrose binding protein in soybean called SBP. These 7S globulins also include soybean allergen beta-conglycinin, peanut allergen conarachin (Ara h 1), walnut allergen Jug r 2, and lentil allergen Len c 1. Proteins in this family perform various functions, including a role in sucrose binding, desiccation, defense against microbes and oxidative stress. The vicilin peptides formed by trypsin or chymotrypsin digestion exhibit antihypertensive effects. These plant seed storage globulins have tandem cupin-like beta-barrel folds (referred to as a bicupin). Storage proteins are the cause of well-known allergic reactions to peanuts and cereals. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380371  Cd Length: 178  Bit Score: 38.26  E-value: 1.07e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15218535  96 VIPMHTHpgASEVLFVLTGSITAGFVSSANAVYVQtLKPGQVMVFPQGLLHFQINAGKSS----ASAVVTFNSANPG 168
Cdd:cd02244  41 FLPHHLD--ADMVFYVHTGRGTITWVDEDKRESYN-LERGDVYRIPAGSTFYLVNTDENEklriIALFDPVNSLTPG 114
COG3837 COG3837
Uncharacterized conserved protein, cupin superfamily [Function unknown];
78-160 1.24e-03

Uncharacterized conserved protein, cupin superfamily [Function unknown];


Pssm-ID: 443048 [Multi-domain]  Cd Length: 115  Bit Score: 37.30  E-value: 1.24e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  78 GLNGLGLSTARLdlAP-KGVIPMHTHPGASEVLFVLTGSITagfVSSANAVYVqtLKPGQVMVFPQGLLHFQINAGKSSA 156
Cdd:COG3837  24 GLTRLGVNLITL--PPgASSSPYHAHSAEEEFVYVLEGELT---LRIGGEEYV--LEPGDSVGFPAGVPHRLRNRGDEPA 96


                ....
gi 15218535 157 SAVV 160
Cdd:COG3837  97 RYLV 100
cupin_DRT102 cd06989
Arabidopsis thaliana DRT102 and related proteins, cupin domain; This family includes bacterial ...
 83-147 1.45e-03

Arabidopsis thaliana DRT102 and related proteins, cupin domain; This family includes bacterial and eukaryotic proteins homologous to DNA-damage-repair/toleration protein DRT102 found in Arabidopsis thaliana. DRT102 may be involved in DNA repair from UV damage. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380394  Cd Length: 97  Bit Score: 36.74  E-value: 1.45e-03
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15218535  83 GLSTARLDLAPKGVIPMHTHPgASEVLFVLTGSITAGFVSSANAVYVQTLKPGQVMVFPQGLLHF 147
Cdd:cd06989  17 GPFVVRLKFPAGYKIPPHTHP-DDERVTVISGTFYLGMGDKFDEAKAKALPAGSFFTLPAGTPHF 80
cupin_PA3510-like cd02225
Pseudomonas aeruginosa PA3510 and related proteins, cupin domain; This family includes ...
 98-169 3.68e-03

Pseudomonas aeruginosa PA3510 and related proteins, cupin domain; This family includes bacterial proteins homologous to PA3510, a Pseudomonas aeruginosa protein of unknown function with a beta-barrel fold that belongs to the cupin superfamily.


Pssm-ID: 380354  Cd Length: 150  Bit Score: 36.49  E-value: 3.68e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15218535  98 PMHTHPgASEVLFVLTGSITAGFVSSANAVYVQtLKPGQVMVFPQGLLHFQINAGKSSASAVVTFNSANPGL 169
Cdd:cd02225  67 ALHTHE-VEEVFFVLQGRLTVFWEDEGEEHERE-LGPRDMISVPAGVYRGFKNIGEEDALMQVMLGTGKPGR 136
cupin_TcmJ-like cd06991
TcmJ monooxygenase and related proteins, cupin domain; This family includes TcmJ, a subunit of ...
 89-161 5.00e-03

TcmJ monooxygenase and related proteins, cupin domain; This family includes TcmJ, a subunit of the tetracenomycin (TCM) polyketide synthase (PKS) type II complex in Streptomyces glaucescens. TcmJ is a quinone-forming monooxygenase involved in the modification of aromatic polyketides synthesized by polyketide synthases of types II and III. Orthologs of TcmJ include the Streptomyces BenD (benastatin biosynthetic pathway), the Streptomyces olivaceus ElmJ (polyketide antibiotic elloramycin biosynthetic pathway), the Actinomadura hibisca PdmL (pradimicin biosynthetic pathway), the Streptomyces cyaneus CurC (curamycin biosynthetic pathway), the Streptomyces rishiriensis Lct30 (lactonamycin biosynthetic pathway), and the Streptomyces WhiE II (spore pigment polyketide biosynthetic pathway). Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380396 [Multi-domain]  Cd Length: 105  Bit Score: 35.35  E-value: 5.00e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15218535  89 LDLAPKGVIPMHTHPGASEVLFVLTGSITAgFVSSAnavyVQTLKPGQVMVFPQGLLHFQINAGKSSASAVVT 161
Cdd:cd06991  24 LTLAPGERVSEHYHPYSEEFLYVVRGRLVV-RVDGE----PVVLEAGEALLVPRGVRHRLENAGDEPARLVFH 91
bicupin_oxalic TIGR03404
bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins ...
 56-201 7.19e-03

bicupin, oxalate decarboxylase family; Members of this protein family are defined as bicupins as they have two copies of the cupin domain (pfam00190). Two different known activities for members of this family are oxalate decarboxylase (EC 4.1.1.2) and oxalate oxidase (EC 1.2.3.4), although the latter activity has more often been found in distantly related monocupin (germin) proteins.


Pssm-ID: 274565 [Multi-domain]  Cd Length: 367  Bit Score: 36.53  E-value: 7.19e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535    56 TPGNTTNIINAAVTP---AFAAqfpglnglglstARLDLAPKGVIPMHTHPGASEVLFVLTGSITAG-FVSSANAvyvQT 131
Cdd:TIGR03404 226 VPGGTVRIADSTNFPvskTIAA------------AIVTVEPGAMRELHWHPNADEWQYFIQGQARMTvFAAGGNA---RT 290

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15218535   132 L--KPGQVMVFPQGLLHFQINAGKSSASAVVTFNSanPGLQILDFALFANSLPTELVVGTTFLDATTVKKLK 201
Cdd:TIGR03404 291 FdyQAGDVGYVPRNMGHYVENTGDETLVFLEVFKA--DRFADVSLNQWLALTPPQLVAAHLNLDDEVIDSLK 360
cupin_XRE_C cd02209
XRE (Xenobiotic Response Element) family transcriptional regulators, C-terminal cupin domain; ...
 76-160 8.09e-03

XRE (Xenobiotic Response Element) family transcriptional regulators, C-terminal cupin domain; This family contains transcriptional regulators containing an N-terminal XRE (Xenobiotic Response Element) family helix-turn-helix (HTH) DNA-binding domain and a C-terminal cupin domain. Included in this family is Escherichia coli transcription factor SutR (YdcN) that plays a regulatory role in sulfur utilization; it regulates a set of genes involved in the generation of sulfate and its reduction, the synthesis of cysteine, the synthesis of enzymes containing Fe-S as cofactors, and the modification of tRNA with use of sulfur-containing substrates. This family belongs to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380339 [Multi-domain]  Cd Length: 90  Bit Score: 34.40  E-value: 8.09e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15218535  76 FPGLNGLGLSTARLDLAPKG-VIPMHTHPGaSEVLFVLTGSITagfVSSANAVYVqtLKPGQVMVFPQGLLHFQINAGKS 154
Cdd:cd02209   8 SPGLPGRKMEPFLVTLPPGGsGGEPYSHEG-EEFGYVLEGELE---LTVGGETYV--LEAGDSIYFDSDVPHRYRNPGDE 81


                ....*.
gi 15218535 155 SASAVV 160
Cdd:cd02209  82 PARVLW 87
cupin_yp_001338853-like cd07008
Klebsiella pneumoniae yp_001338853.1 and related proteins, cupin domain; This family includes ...
 97-153 8.40e-03

Klebsiella pneumoniae yp_001338853.1 and related proteins, cupin domain; This family includes bacterial proteins homologous to Klebsiella pneumoniae yp_001338853.1, an uncharacterized conserved protein with double-stranded beta-helix domain. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380411 [Multi-domain]  Cd Length: 101  Bit Score: 34.54  E-value: 8.40e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 15218535  97 IPMHTHPGASEVLFVLTGsiTAGFVSSANAVyvQTLKPGQVMVFPQGLLHFQINAGK 153
Cdd:cd07008  40 IAAHIHPHGQDTWIVLSG--EGEYLLGDGQT--VPIKAGDIVIAPAGQVHGARNTGD 92
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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