PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 15229771  91 MEIGVPTDVRHVAHVTFDRFHGF-LGLPVEFEPEVPRRAPSA 131
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGVGFDgANLPPDLQSLFQTAGISA 42

RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 155 PTILLMMQSHLYSRGgLRVEGIFRINGENGQEEYIREELNKGIIPDNI---DVHCLASLIKAWFRELPSGVLD-SLSPE- 229
Cdd:cd00159   1 PLIIEKCIEYLEKNG-LNTEGIFRVSGSASKIEELKKKFDRGEDIDDLedyDVHDVASLLKLYLRELPEPLIPfELYDEf 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 230 -----QVMESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPNMTQMLDP-LTALMYAVQV 303
Cdd:cd00159  80 ielakIEDEEERIEALKELLKSLPPENRDLLKYLLKLLHKISQNSEVNKMTASNLAIVFAPTLLRPPDSdDELLEDIKKL 159

                ....*...
gi 15229771 304 MNFLKTLI 311
Cdd:cd00159 160 NEIVEFLI 167

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 15229771  91 MEIGVPTDVRHVAHVTFDRFHGF-LGLPVEFEPEVPRRAPSA 131
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGVGFDgANLPPDLQSLFQTAGISA 42

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).

                          10        20
                  ....*....|....*....|....*..
gi 15229771    92 EIGVPTDVRHVAHVTFDRFHG-FLGLP 117
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGfFTGLP 27

RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 155 PTILLMMQSHLYSRGgLRVEGIFRINGENGQEEYIREELNKGIIPDNI---DVHCLASLIKAWFRELPSGVLD-SLSPE- 229
Cdd:cd00159   1 PLIIEKCIEYLEKNG-LNTEGIFRVSGSASKIEELKKKFDRGEDIDDLedyDVHDVASLLKLYLRELPEPLIPfELYDEf 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 230 -----QVMESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPNMTQMLDP-LTALMYAVQV 303
Cdd:cd00159  80 ielakIEDEEERIEALKELLKSLPPENRDLLKYLLKLLHKISQNSEVNKMTASNLAIVFAPTLLRPPDSdDELLEDIKKL 159

                ....*...
gi 15229771 304 MNFLKTLI 311
Cdd:cd00159 160 NEIVEFLI 167

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 136 FGVSTES-MQL---SYDTRGniVPTILLMMQSHLYSRGGLRVEGIFRINGENGQEEYIREELNKGIIPDN--IDVHCLAS 209
Cdd:cd04389   1 FGSSLEEiMDRqkeKYPELK--LPWILTFLSEKVLALGGFQTEGIFRVPGDIDEVNELKLRVDQWDYPLSglEDPHVPAS 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 210 LIKAWFRELPSGVL-DSLSPEQVMESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQL--NKMNARNIAMVFAPN 286
Cdd:cd04389  79 LLKLWLRELEEPLIpDALYQQCISASEDPDKAVEIVQKLPIINRLVLCYLINFLQVFAQPENVahTKMDVSNLAMVFAPN 158

                       170       180
                ....*....|....*....|....*...
gi 15229771 287 M--TQMLDPLTALMYAVQVMNFLKTLIV 312
Cdd:cd04389 159 IlrCTSDDPRVIFENTRKEMSFLRTLIE 186

RhoGAP_FAM13A1a: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of FAM13A1, isoform a-like proteins. The function of FAM13A1a is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by up several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 135 VFGVSTESMQLSYDTRgNIVPTILLMMQSHLySRGGLRVEGIFRINGENGQEEYIREELNKGIIPD---NIDVHCLASLI 211
Cdd:cd04393   2 VFGVPLQELQQAGQPE-NGVPAVVRHIVEYL-EQHGLEQEGLFRVNGNAETVEWLRQRLDSGEEVDlskEADVCSAASLL 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 212 KAWFRELPSGVLDSLSPEQVM--------ESESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVF 283
Cdd:cd04393  80 RLFLQELPEGLIPASLQIRLMqlyqdyngEDEFGRKLRDLLQQLPPVNYSLLKFLCHFLSNVASQHHENRMTAENLAAVF 159

                ...
gi 15229771 284 APN 286
Cdd:cd04393 160 GPD 162

RhoGAP_fRGD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 136 FGVSTESMqlsYDTRGNIVPTILL--MMQSHLYsrgGLRVEGIFRINGENGQEEYIREELNKGiiPDNI----------D 203
Cdd:cd04398   1 FGVPLEDL---ILREGDNVPNIVYqcIQAIENF---GLNLEGIYRLSGNVSRVNKLKELFDKD--PLNVllispedyesD 72

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 204 VHCLASLIKAWFRELPSGVL-DSLSPEQVMESESEDECV------ELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNA 276
Cdd:cd04398  73 IHSVASLLKLFFRELPEPLLtKALSREFIEAAKIEDESRrrdalhGLINDLPDANYATLRALMFHLARIKEHESVNRMSV 152

                ....*....
gi 15229771 277 RNIAMVFAP 285
Cdd:cd04398 153 NNLAIIWGP 161

RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for Rac1 and Cdc42. Short isoforms (without the PH domain) of ARHGAP24, called RC-GAP72 and p73RhoGAP, and of ARHGAP22, called p68RacGAP, has been shown to be involved in angiogenesis and endothelial cell capillary formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 170 GLRVEGIFRINGENGQEEYIREELNKGIIP---DNIDVHCLASLIKAWFRELPSGVL------DSLSPEQVMESESEDEC 240
Cdd:cd04390  37 GLKEEGLFRLPGQANLVKQLQDAFDAGERPsfdSDTDVHTVASLLKLYLRELPEPVIpwaqyeDFLSCAQLLSKDEEKGL 116

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15229771 241 VEL---VRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPNM--TQMLDPLTALMYAVQVMNFLKTLIVKT 314
Cdd:cd04390 117 GELmkqVSILPKVNYNLLSYICRFLDEVQSNSSVNKMSVQNLATVFGPNIlrPKVEDPATIMEGTPQIQQLMTVMISKH 195

RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ArfGAP activity is PIP3-dependent, ARAPs are considered integration points for phosphoinositide, Arf and Rho signaling. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 137 GVSTESMQLsydTRGNIvPTILLMMQSHLYsRGGLRVEGIFRINGENGQ-----EEYIREELNKGIIPDNIDVHCLASLI 211
Cdd:cd04385   2 GPALEDQQL---TDNDI-PVIVDKCIDFIT-QHGLMSEGIYRKNGKNSSvkkllEAFRKDARSVQLREGEYTVHDVADVL 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 212 KAWFRELPSGVL-DSLSPEQVMESESEDECV------ELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFA 284
Cdd:cd04385  77 KRFLRDLPDPLLtSELHAEWIEAAELENKDEriarykELIRRLPPINRATLKVLIGHLYRVQKHSDENQMSVHNLALVFG 156

                       170       180
                ....*....|....*....|.
gi 15229771 285 PN-MTQMLDPLTALMYAVQVM 304
Cdd:cd04385 157 PTlFQTDEHSVGQTSHEVKVI 177

RhoGap_RalBP1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in RalBP1 proteins, also known as RLIP, RLIP76 or cytocentrin. RalBP1 plays an important role in endocytosis during interphase. During mitosis, RalBP1 transiently associates with the centromere and has been shown to play an essential role in the proper assembly of the mitotic apparatus. RalBP1 is an effector of the Ral GTPase which itself is an effector of Ras. RalBP1 contains a RhoGAP domain, which shows weak activity towards Rac1 and Cdc42, but not towards Ral, and a Ral effector domain binding motif. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 170 GLRVEGIFRINGENGQEEYIREELNKGIIP--DNIDVHCLASLIKAWFRELPSGVLDS-LSP--EQVM----ESESEDEC 240
Cdd:cd04381  35 GMKCEGIYKVSGIKSKVDELKAAYNRRESPnlEEYEPPTVASLLKQYLRELPEPLLTKeLMPrfEEACgrptEAEREQEL 114

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*
gi 15229771 241 VELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAP 285
Cdd:cd04381 115 QRLLKELPECNRLLLAWLIVHMDHVIAQELETKMNIQNISIVLSP 159

RhoGAP-ARHGAP11A: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP11A-like proteins. The mouse homolog of human ArhGAP11A has been detected as a gene exclusively expressed in immature ganglion cells, potentially playing a role in retinal development. The exact function of ArhGAP11A is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 135 VFGVSTESMQLSYDTRGNIVPTILLMMQSHLYSRggLRVEGIFRINGENGQEEYIREELNKGIIPDN----IDVhclASL 210
Cdd:cd04394   1 VFGVPLHSLPHSTVPEYGNVPKFLVDACTFLLDH--LSTEGLFRKSGSVVRQKELKAKLEGGEACLSsalpCDV---AGL 75

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 211 IKAWFRELPSGVL-----DSLSPEQVMESESE--DECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVF 283
Cdd:cd04394  76 LKQFFRELPEPLLpydlhEALLKAQELPTDEErkSATLLLTCLLPDEHVNTLRYFFSFLYDVAQRCSENKMDSSNLAVIF 155

                       170       180       190
                ....*....|....*....|....*....|....
gi 15229771 284 APNMTQMLDPLTALMYAVQVMNFLKTLIVKTLKD 317
Cdd:cd04394 156 APNLFQSEEGGEKMSSSTEKRLRLQAAVVQTLID 189

RhoGAP_myosin_IXB: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 136 FGVSTESMQlsydTRGNIVPTILLMMQSHLySRGGLRVEGIFRINGENGQEEYIREELNKG---IIPDNIDVHCLASLIK 212
Cdd:cd04407   1 FGVRVGSLT----SNKTSVPIVLEKLLEHV-EMHGLYTEGIYRKSGSANRMKELHQLLQADpenVKLENYPIHAITGLLK 75

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 213 AWFRELPSGVL------DSLSPEQVMESESEDECV-ELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAP 285
Cdd:cd04407  76 QWLRELPEPLMtfaqynDFLRAVELPEKQEQLQAIyRVLEQLPTANHNTLERLIFHLVKVALEEDVNRMSPNALAIVFAP 155

                       170       180       190
                ....*....|....*....|....*....|.
gi 15229771 286 NMTQ---MLDPLTALMYAVQVMNFLKTLIVK 313
Cdd:cd04407 156 CLLRcpdSSDPLTSMKDVAKTTTCVEMLIKE 186

RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 154 VPTILLMMQSHLySRGGLRVEGIFRINGENGQEEYIREELNKGI---IPDNIDVHCLASLIKAWFRELPSGVL------- 223
Cdd:cd04376   9 VPRLVESCCQHL-EKHGLQTVGIFRVGSSKKRVRQLREEFDRGIdvvLDENHSVHDVAALLKEFFRDMPDPLLprelyta 87

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 224 ----DSLSPEQVMESESEdecveLVRLLPSTEASLLDWAINLMADVV-----------EMEQLNKMNARNIAMVFAPNMT 288
Cdd:cd04376  88 figtALLEPDEQLEALQL-----LIYLLPPCNCDTLHRLLKFLHTVAehaadsidedgQEVSGNKMTSLNLATIFGPNLL 162

                .
gi 15229771 289 Q 289
Cdd:cd04376 163 H 163

RhoGAP_chimaerin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of chimaerins. Chimaerins are a family of phorbolester- and diacylglycerol-responsive GAPs specific for the Rho-like GTPase Rac. Chimaerins exist in two alternative splice forms that each contain a C-terminal GAP domain, and a central C1 domain which binds phorbol esters, inducing a conformational change that activates the protein; one splice form is lacking the N-terminal Src homology-2 (SH2) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 170 GLRVEGIFRINGENGQEEYIREELN----KGIIPDNI--DVHCLASLIKAWFRELPSGVLDSLSPEQVMES---ESEDEC 240
Cdd:cd04372  31 GLQSEGLYRVSGFAEEIEDVKMAFDrdgeKADISATVypDINVITGALKLYFRDLPIPVITYDTYPKFIDAakiSNPDER 110

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15229771 241 VELV----RLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPnmTQMLDPLTALMYAVQVMNFLKtLIVKTL 315
Cdd:cd04372 111 LEAVhealMLLPPAHYETLRYLMEHLKRVTLHEKDNKMNAENLGIVFGP--TLMRPPEDSALTTLNDMRYQI-LIVQLL 186

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 15229771  91 MEIGVPTDVRHVAHVTFDRFHGF-LGLPVEFEPEVPRRAPSA 131
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGVGFDgANLPPDLQSLFQTAGISA 42

RhoGAP_GMIP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein) and PARG1 (PTPL1-associated RhoGAP1). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 154 VPTILLMMQSHLYSRGgLRVEGIFRINGENGQEEYIREELNKGiiPDNIDV-----HCLASLIKAWFRELPSGVLD---- 224
Cdd:cd04378  16 VPFIIKKCTSEIENRA-LGVQGIYRVSGSKARVEKLCQAFENG--KDLVELselspHDISSVLKLFLRQLPEPLILfrly 92

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 225 ----SLSPE----------QVMESESEDECV---ELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNARNIAMVFAPNM 287
Cdd:cd04378  93 ndfiALAKEiqrdteedkaPNTPIEVNRIIRklkDLLRQLPASNYNTLQHLIAHLYRVAEQFEENKMSPNNLGIVFGPTL 172

                       170       180
                ....*....|....*....|....*...
gi 15229771 288 TQMLD-----PLTALM---YAVQVMNFL 307
Cdd:cd04378 173 IRPRPgdadvSLSSLVdygYQARLVEFL 200

RhoGAP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of PARG1 (PTPL1-associated RhoGAP1). PARG1 was originally cloned as an interaction partner of PTPL1, an intracellular protein-tyrosine phosphatase. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 154 VPTILLMMQSHLYSRGgLRVEGIFRINGENGQEEYIREELNKGIipDNIDV-----HCLASLIKAWFRELP--------- 219
Cdd:cd04409  16 IPFIIKKCTSEIESRA-LCLKGIYRVNGAKSRVEKLCQAFENGK--DLVELselspHDISNVLKLYLRQLPeplilfrly 92

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 220 SGVLD------SLSPEQVMESESEDE----CVELVRL----------LPSTEASLLDWAINLMADVVEMEQLNKMNARNI 279
Cdd:cd04409  93 NEFIGlakesqHVNETQEAKKNSDKKwpnmCTELNRIllkskdllrqLPAPNYNTLQFLIVHLHRVSEQAEENKMSASNL 172

                ....*...
gi 15229771 280 AMVFAPNM 287
Cdd:cd04409 173 GIIFGPTL 180

RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 170 GLRVEGIFRINGENGQEEYIREELNKG-IIPD--NIDVHCLASLIKAWFRELPSGVLD-SLSPEQVMESESEDEC----- 240
Cdd:cd04382  32 GLTEEGLYRVSGSEREVKALKEKFLRGkTVPNlsKVDIHVICGCLKDFLRSLKEPLITfALWKEFMEAAEILDEDnsraa 111

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 15229771 241 -VELVRLLPSTEASLLDWAInLMADVVEMEQLNKMNARNIAMVFAP 285
Cdd:cd04382 112 lYQAISELPQPNRDTLAFLI-LHLQRVAQSPECKMDINNLARVFGP 156

RhoGAP_SYD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in SYD-1_like proteins. Syd-1, first identified and best studied in C.elegans, has been shown to play an important role in neuronal development by specifying axonal properties. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 136 FGVSTESMQLSYDTRGNiVPtILLMMQSHLYSRGGLRVEGIFRINGENGQEEYIREELNKG-----IIPDNI-DVHCLAS 209
Cdd:cd04379   1 FGVPLSRLVEREGESRD-VP-IVLQKCVQEIERRGLDVIGLYRLCGSAAKKKELRDAFERNsaaveLSEELYpDINVITG 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 210 LIKAWFRELPSG---------VLDSL---SPEQVmeSESEDECVELVRLLPSTEASLLDWAINLMADVVEMEQLNKMNAR 277
Cdd:cd04379  79 VLKDYLRELPEPlitpqlyemVLEALavaLPNDV--QTNTHLTLSIIDCLPLSAKATLLLLLDHLSLVLSNSERNKMTPQ 156

                ....*...
gi 15229771 278 NIAMVFAP 285
Cdd:cd04379 157 NLAVCFGP 164

RhoGAP_fLRG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal LRG1-like proteins. Yeast Lrg1p is required for efficient cell fusion, and mother-daughter cell separation, possibly through acting as a RhoGAP specifically regulating 1,3-beta-glucan synthesis. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 171 LRVEGIFRINGENGQEEYIREELNKGiiPDNI------DVHCLASLIKAWFRELPSGVLD-SLSPEQVMESESEDE---- 239
Cdd:cd04397  43 MSVEGVFRKNGNIRRLKELTEEIDKN--PTEVpdlskeNPVQLAALLKKFLRELPDPLLTfKLYRLWISSQKIEDEeerk 120

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 240 -CVELVR-LLP-----STEA--SLLDWAINLMAdvVEMEQLNKMNARNIAMVFAPNM----TQMLDPLTALMYAVQVMNF 306
Cdd:cd04397 121 rVLHLVYcLLPkyhrdTMEVlfSFLKWVSSFSH--IDEETGSKMDIHNLATVITPNIlyskTDNPNTGDEYFLAIEAVNY 198

                ....*
gi 15229771 307 LKTLI 311
Cdd:cd04397 199 LIENN 203

RhoGAP_GMIP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 136 FGVstESMQLSYDTRGNiVPTILLMMQSHLYSRGgLRVEGIFRINGENGQEEYIREELNKGiiPDNIDV-----HCLASL 210
Cdd:cd04408   1 FGV--DFSQLPRDFPEE-VPFVVVRCTAEIENRA-LGVQGIYRISGSKARVEKLCQAFENG--RDLVDLsghspHDITSV 74

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15229771 211 IKAWFRELPSGVLD--------SLSPEQVMESESEDECV-----------ELVRLLPSTEASLLDWAINLMADVVEMEQL 271
Cdd:cd04408  75 LKHFLKELPEPVLPfqlyddfiALAKELQRDSEKAAESPsiveniirslkELLGRLPVSNYNTLRHLMAHLYRVAERFED 154

                       170       180       190       200
                ....*....|....*....|....*....|....*....|...
gi 15229771 272 NKMNARNIAMVFAPNMTQMLD----PLTALM---YAVQVMNFL 307
Cdd:cd04408 155 NKMSPNNLGIVFGPTLLRPLVggdvSMICLLdtgYQAQLVEFL 197

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).

                          10        20
                  ....*....|....*....|....*..
gi 15229771    92 EIGVPTDVRHVAHVTFDRFHG-FLGLP 117
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGfFTGLP 27