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Conserved domains on  [gi|15231328|ref|NP_187980|]
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F-box and associated interaction domains-containing protein [Arabidopsis thaliana]

Protein Classification

F-box protein( domain architecture ID 17779305)

F-box protein functions as the substrate-recognition component of an SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligase that is involved in ubiquitin-dependent degradation

CATH:  1.20.1280.50
Gene Ontology:  GO:0006511|GO:0019005|GO:0005515
PubMed:  31898225|11178263
SCOP:  4001927

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FBA_1 pfam07734
F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that ...
 197-379 1.69e-66

F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that they are effectors linked with ubiquitination.


:

Pssm-ID: 254394  Cd Length: 159  Bit Score: 208.23  E-value: 1.69e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   197 GASLKGNAYFPAQKKETvggiktiNIEDVLLCFDFTRERFGPPLPLPFHSYNADfFVSLACVREEQLAVLYQRWGAFEtI 276
Cdd:pfam07734   1 GVSLKGNTYWLAREKET-------NEEDFLLSFDFTTERFGPRLPLPFQSPDLD-TVSLSVVREEKLAVLLQEDDTSK-I 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   277 EICVTNKIDPNTVSWSKFLITS----TGFPVDTFSGSFFIDEEKKVAVVFDLDRYKptetcRYQIVYIIGQDEYFKSVNm 352
Cdd:pfam07734  72 EIWVTTKIEPNAVSWSKFLTVDmpplPGLFFHFFAGSFFIDEEKKVAVVCDKDKEK-----KTNTIYIIGEDGYFKEVD- 145

                         170       180
                  ....*....|....*....|....*..
gi 15231328   353 gvapniaknsyQAYCVPLVCssSYVPS 379
Cdd:pfam07734 146 -----------SSYCSPLVC--SYVPS 159
F-box_AtFBW1-like cd22157
F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) ...
 6-41 2.42e-11

F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) and similar proteins; AtFBW1, also called WD-40-associated F-box protein 1, is an F-box protein that contains four WD-40 repeats, which are separated from each other by a spacer region. Like other F-box proteins, AtFBW1 may be a component of SCF (Skp1 Cdc53 F-box protein) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. This subfamily also contains many F-box only proteins that do not have any WD repeat. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


:

Pssm-ID: 438928  Cd Length: 39  Bit Score: 57.86  E-value: 2.42e-11
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 15231328   6 DLPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQ 41
Cdd:cd22157   2 SLPDDLVEEILSRLPAKSLLRFRCVCKQWNSLISSP 37
 
Name Accession Description Interval E-value
FBA_1 pfam07734
F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that ...
 197-379 1.69e-66

F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that they are effectors linked with ubiquitination.


Pssm-ID: 254394  Cd Length: 159  Bit Score: 208.23  E-value: 1.69e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   197 GASLKGNAYFPAQKKETvggiktiNIEDVLLCFDFTRERFGPPLPLPFHSYNADfFVSLACVREEQLAVLYQRWGAFEtI 276
Cdd:pfam07734   1 GVSLKGNTYWLAREKET-------NEEDFLLSFDFTTERFGPRLPLPFQSPDLD-TVSLSVVREEKLAVLLQEDDTSK-I 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   277 EICVTNKIDPNTVSWSKFLITS----TGFPVDTFSGSFFIDEEKKVAVVFDLDRYKptetcRYQIVYIIGQDEYFKSVNm 352
Cdd:pfam07734  72 EIWVTTKIEPNAVSWSKFLTVDmpplPGLFFHFFAGSFFIDEEKKVAVVCDKDKEK-----KTNTIYIIGEDGYFKEVD- 145

                         170       180
                  ....*....|....*....|....*..
gi 15231328   353 gvapniaknsyQAYCVPLVCssSYVPS 379
Cdd:pfam07734 146 -----------SSYCSPLVC--SYVPS 159
F_box_assoc_1 TIGR01640
F-box protein interaction domain; This model describes a large family of plant domains, with ...
 100-325 1.72e-43

F-box protein interaction domain; This model describes a large family of plant domains, with several hundred members in Arabidopsis thaliana. Most examples are found C-terminal to an F-box (pfam00646), a 60 amino acid motif involved in ubiquitination of target proteins to mark them for degradation. Two-hybid experiments support the idea that most members are interchangeable F-box subunits of SCF E3 complexes. Some members have two copies of this domain.


Pssm-ID: 273726 [Multi-domain]  Cd Length: 230  Bit Score: 150.97  E-value: 1.72e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   100 VFHCDGLvLCIIKDNTgLLVWNPYLGQTRWIHPRNN---YQIEDRYALGYDN-NRNHKILRIFDLYPSSNRvFGYEVYDF 175
Cdd:TIGR01640   1 VVPCDGL-ICFSYGKR-LVVWNPSTGQSRWLPTPKSrrsNKESDTYFLGYDPiEKQYKVLCFSDRSGNRNQ-SEHQVYTL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   176 SSNSWKVLDVIPEWDIQSHfRGASLKGNAYFPAQKKETvggiktiNIEDVLLCFDFTRERFGPPLPLPFHSYNADFFVSL 255
Cdd:TIGR01640  78 GSNSWRTIECSPPHHPLKS-RGVCINGVLYYLAYTLKT-------NPDYFIVSFDVSSERFKEFIPLPCGNSDSVDYLSL 149

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15231328   256 AcVREEQLAVLYQRWGAfETIEICVTNkiDPNTVSWSKFL-ITSTGFP--VDTFSGSFFIDEEKKVAVVFDLD 325
Cdd:TIGR01640 150 I-NYKGKLAVLKQKKDT-NNFDLWVLN--DAGKQEWSKLFtVPIPPLPdlVDDNFLSGFTDKGEIVLCCEDEN 218
F-box_AtFBW1-like cd22157
F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) ...
 6-41 2.42e-11

F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) and similar proteins; AtFBW1, also called WD-40-associated F-box protein 1, is an F-box protein that contains four WD-40 repeats, which are separated from each other by a spacer region. Like other F-box proteins, AtFBW1 may be a component of SCF (Skp1 Cdc53 F-box protein) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. This subfamily also contains many F-box only proteins that do not have any WD repeat. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438928  Cd Length: 39  Bit Score: 57.86  E-value: 2.42e-11
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 15231328   6 DLPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQ 41
Cdd:cd22157   2 SLPDDLVEEILSRLPAKSLLRFRCVCKQWNSLISSP 37
FBOX smart00256
A Receptor for Ubiquitination Targets;
7-46 8.03e-07

A Receptor for Ubiquitination Targets;


Pssm-ID: 197608  Cd Length: 41  Bit Score: 45.12  E-value: 8.03e-07
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|
gi 15231328      7 LPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQICGRK 46
Cdd:smart00256   1 LPDEILEEILSKLDPKDLLRLRKVSRKWRSLIDSHDFWFK 40
F-box pfam00646
F-box domain; This domain is approximately 50 amino acids long, and is usually found in the ...
 6-46 2.27e-06

F-box domain; This domain is approximately 50 amino acids long, and is usually found in the N-terminal half of a variety of proteins. Two motifs that are commonly found associated with the F-box domain are the leucine rich repeats (LRRs; pfam00560 and pfam07723) and the WD repeat (pfam00400). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 425796  Cd Length: 43  Bit Score: 44.07  E-value: 2.27e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 15231328     6 DLPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQICGRK 46
Cdd:pfam00646   3 DLPDDLLLEILSRLDPKDLLRLSLVSKRWRSLVDSLKLWKK 43
 
Name Accession Description Interval E-value
FBA_1 pfam07734
F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that ...
 197-379 1.69e-66

F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that they are effectors linked with ubiquitination.


Pssm-ID: 254394  Cd Length: 159  Bit Score: 208.23  E-value: 1.69e-66
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   197 GASLKGNAYFPAQKKETvggiktiNIEDVLLCFDFTRERFGPPLPLPFHSYNADfFVSLACVREEQLAVLYQRWGAFEtI 276
Cdd:pfam07734   1 GVSLKGNTYWLAREKET-------NEEDFLLSFDFTTERFGPRLPLPFQSPDLD-TVSLSVVREEKLAVLLQEDDTSK-I 71

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   277 EICVTNKIDPNTVSWSKFLITS----TGFPVDTFSGSFFIDEEKKVAVVFDLDRYKptetcRYQIVYIIGQDEYFKSVNm 352
Cdd:pfam07734  72 EIWVTTKIEPNAVSWSKFLTVDmpplPGLFFHFFAGSFFIDEEKKVAVVCDKDKEK-----KTNTIYIIGEDGYFKEVD- 145

                         170       180
                  ....*....|....*....|....*..
gi 15231328   353 gvapniaknsyQAYCVPLVCssSYVPS 379
Cdd:pfam07734 146 -----------SSYCSPLVC--SYVPS 159
F_box_assoc_1 TIGR01640
F-box protein interaction domain; This model describes a large family of plant domains, with ...
 100-325 1.72e-43

F-box protein interaction domain; This model describes a large family of plant domains, with several hundred members in Arabidopsis thaliana. Most examples are found C-terminal to an F-box (pfam00646), a 60 amino acid motif involved in ubiquitination of target proteins to mark them for degradation. Two-hybid experiments support the idea that most members are interchangeable F-box subunits of SCF E3 complexes. Some members have two copies of this domain.


Pssm-ID: 273726 [Multi-domain]  Cd Length: 230  Bit Score: 150.97  E-value: 1.72e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   100 VFHCDGLvLCIIKDNTgLLVWNPYLGQTRWIHPRNN---YQIEDRYALGYDN-NRNHKILRIFDLYPSSNRvFGYEVYDF 175
Cdd:TIGR01640   1 VVPCDGL-ICFSYGKR-LVVWNPSTGQSRWLPTPKSrrsNKESDTYFLGYDPiEKQYKVLCFSDRSGNRNQ-SEHQVYTL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15231328   176 SSNSWKVLDVIPEWDIQSHfRGASLKGNAYFPAQKKETvggiktiNIEDVLLCFDFTRERFGPPLPLPFHSYNADFFVSL 255
Cdd:TIGR01640  78 GSNSWRTIECSPPHHPLKS-RGVCINGVLYYLAYTLKT-------NPDYFIVSFDVSSERFKEFIPLPCGNSDSVDYLSL 149

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15231328   256 AcVREEQLAVLYQRWGAfETIEICVTNkiDPNTVSWSKFL-ITSTGFP--VDTFSGSFFIDEEKKVAVVFDLD 325
Cdd:TIGR01640 150 I-NYKGKLAVLKQKKDT-NNFDLWVLN--DAGKQEWSKLFtVPIPPLPdlVDDNFLSGFTDKGEIVLCCEDEN 218
F-box_AtFBW1-like cd22157
F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) ...
 6-41 2.42e-11

F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) and similar proteins; AtFBW1, also called WD-40-associated F-box protein 1, is an F-box protein that contains four WD-40 repeats, which are separated from each other by a spacer region. Like other F-box proteins, AtFBW1 may be a component of SCF (Skp1 Cdc53 F-box protein) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. This subfamily also contains many F-box only proteins that do not have any WD repeat. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438928  Cd Length: 39  Bit Score: 57.86  E-value: 2.42e-11
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 15231328   6 DLPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQ 41
Cdd:cd22157   2 SLPDDLVEEILSRLPAKSLLRFRCVCKQWNSLISSP 37
FBOX smart00256
A Receptor for Ubiquitination Targets;
7-46 8.03e-07

A Receptor for Ubiquitination Targets;


Pssm-ID: 197608  Cd Length: 41  Bit Score: 45.12  E-value: 8.03e-07
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|
gi 15231328      7 LPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQICGRK 46
Cdd:smart00256   1 LPDEILEEILSKLDPKDLLRLRKVSRKWRSLIDSHDFWFK 40
F-box pfam00646
F-box domain; This domain is approximately 50 amino acids long, and is usually found in the ...
 6-46 2.27e-06

F-box domain; This domain is approximately 50 amino acids long, and is usually found in the N-terminal half of a variety of proteins. Two motifs that are commonly found associated with the F-box domain are the leucine rich repeats (LRRs; pfam00560 and pfam07723) and the WD repeat (pfam00400). The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 425796  Cd Length: 43  Bit Score: 44.07  E-value: 2.27e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 15231328     6 DLPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQICGRK 46
Cdd:pfam00646   3 DLPDDLLLEILSRLDPKDLLRLSLVSKRWRSLVDSLKLWKK 43
F-box_SF cd09917
F-box domain superfamily; This short domain is commonly found at the N-terminus of various ...
 6-39 1.85e-04

F-box domain superfamily; This short domain is commonly found at the N-terminus of various proteins, and typically co-occurs with one or more other conserved domains or motifs, such as leucine rich repeats, WD40 repeats, kelch, tub, spry, and others. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression. One of the best researched roles of F-box proteins is their participation in SCF (Skp1-Cul1-F-box protein), a multi-protein complex that functions as a ubiquitin E3 ligase, where the role of the F-box protein is to recruit target substrates. Gene families containing the F-box are found greatly expanded in narrow taxonomic lineages, such as flowering plants and nematodes. In this hierarchical classification, many of the subfamilies are named according to their domain architectures.


Pssm-ID: 438852  Cd Length: 35  Bit Score: 38.58  E-value: 1.85e-04
                        10        20        30
                ....*....|....*....|....*....|....
gi 15231328   6 DLPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSK 39
Cdd:cd09917   2 DLPDEILLKILSYLDPRDLLRLSLVCKRWRELAS 35
F-box_AtAFR-like cd22152
F-box domain found in Arabidopsis thaliana protein ATTENUATED FAR-RED RESPONSE (AtAFR) and ...
 7-41 8.99e-04

F-box domain found in Arabidopsis thaliana protein ATTENUATED FAR-RED RESPONSE (AtAFR) and similar proteins; AtAFR, also called SKP1-interacting partner 29 (AtSKIP29), or F-box protein AFR, is a component of SCF (SKP1/ASK-cullin-F-box protein) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. It is part of the phyA-mediated signaling transduction pathway leading to the regulation of gene expression and hypocotyl elongation in response to red and far-red light exposure. This subfamily also includes many other Arabidopsis thaliana SKP1-interacting partner proteins. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438923  Cd Length: 45  Bit Score: 36.78  E-value: 8.99e-04
                        10        20        30
                ....*....|....*....|....*....|....*
gi 15231328   7 LPGDLVEEILSRVPLTSLRAIRSTCQKWNSLSKSQ 41
Cdd:cd22152   5 LPDDIALQCLARVPRSSHPSLSLVSKSWRSLLSSP 39
F-box_FBXO6-like cd22168
F-box domain found in F-box only proteins FBXO6, FBXO44 and similar proteins; This subfamily ...
 1-37 9.44e-04

F-box domain found in F-box only proteins FBXO6, FBXO44 and similar proteins; This subfamily includes FBXO6 and FBXO44. FBXO6, also called FBX6, F-box protein that recognizes sugar chains 2 (FBS2), or F-box/G-domain protein 2 (FBG2), is a substrate-recognition component of SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complexes. It is involved in the endoplasmic reticulum-associated degradation pathway (ERAD) for misfolded lumenal proteins, by recognizing and binding sugar chains on unfolded glycoproteins that are retro-translocated into the cytosol and promoting their ubiquitination and subsequent degradation. FBXO6 can recognize and bind denatured glycoproteins, which are modified with not only high-mannose but also complex-type oligosaccharides. It also recognizes sulfated glycans. FBXO6 is involved in DNA damage response by specifically recognizing activated CHEK1 (phosphorylated on 'Ser-345'), promoting its ubiquitination and degradation. FBXO44, also called FBXO6A, FBX44, or F-box/G-domain protein 3 (FBG3), is a substrate-recognition component of an SCF-type E3 ubiquitin ligase complex. It interacts with SKP1 and CUL1. FBXO44 mediates BRCA1 ubiquitination and degradation. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438939 [Multi-domain]  Cd Length: 82  Bit Score: 37.65  E-value: 9.44e-04
                        10        20        30
                ....*....|....*....|....*....|....*...
gi 15231328   1 MTTMGDLPGDLVEEILSRVPLTSL-RAIRSTCQKWNSL 37
Cdd:cd22168   1 MLTISDLPEDVLLEILSLVPARDLiLSCRLVCSRWRDL 38
F-box_FBA cd22083
F-box domain found in the F-box associated (FBA) family of F-box proteins; The F-box ...
 7-37 5.83e-03

F-box domain found in the F-box associated (FBA) family of F-box proteins; The F-box associated (FBA) family is composed of FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44, which contain a conserved G domain that mediates substrate binding. Members of this family play diverse roles in glycoprotein quality control. They bind high mannose and sulfated glycoproteins, with one FBA protein, FBXO44, failing to bind any glycans on the tested arrays. FBA proteins are components of canonical SCF (Skp1, Cullin1, and Rbx1) complexes, yet FBXO2 bound very little Cullin1, suggesting that FBXO2 may exist primarily as a heterodimer with Skp1. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438855  Cd Length: 37  Bit Score: 34.29  E-value: 5.83e-03
                        10        20        30
                ....*....|....*....|....*....|..
gi 15231328   7 LPGDLVEEILSRVPLTSL-RAIRSTCQKWNSL 37
Cdd:cd22083   4 IPEELLLEILTHVPATSLiTNCRLVCTLWRDI 35
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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