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Conserved domains on  [gi|15238418|ref|NP_196130|]
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Cystatin/monellin family protein [Arabidopsis thaliana]

Protein Classification

cystatin family protein( domain architecture ID 430)

cystatin family protein may function as a cysteine protease inhibitor, such as Glycine max cystatin that inhibits papain and cathepsin B

CATH:  3.10.450.10
Gene Ontology:  GO:0004869|GO:0010466
PubMed:  1855589|9007972|3555466|14587292
SCOP:  4001013

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
CY super family cl09238
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 61-132 5.36e-21

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


The actual alignment was detected with superfamily member pfam16845:

Pssm-ID: 447698  Cd Length: 83  Bit Score: 83.63  E-value: 5.36e-21
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15238418    61 IDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVI-EAGEKKIYEAKVWVKPWMNFKQLQEFK 132
Cdd:pfam16845   9 VVEIAKFAVSEHNKQSKANLVFVKVVKGETQVVSGTNYRLVIAAKdGGGATKNYEAVVWEKPWEHFRSLTSFK 81
CY super family cl09238
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 154-214 5.59e-05

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


The actual alignment was detected with superfamily member smart00043:

Pssm-ID: 447698  Cd Length: 107  Bit Score: 41.26  E-value: 5.59e-05
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15238418    154 WRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPYKLIDIILARAKVVeERVKFELLLKLERGN 214
Cdd:smart00043   6 PSDVPPNDPEVQEAADFAVAEYNKKSNDKYELRVIKVVSAKSQVV-AGTNYYLKVEVGETN 65
 
Name Accession Description Interval E-value
SQAPI pfam16845
Aspartic acid proteinase inhibitor; SQAPI, aspartic acid inhibitor first isolated from squash, ...
 61-132 5.36e-21

Aspartic acid proteinase inhibitor; SQAPI, aspartic acid inhibitor first isolated from squash, inhibits a wide range of aspartic proteinases. This particular family of PAAPIs (proteinaceous aspartic acid inhibitors) seems to have evolved quite recently from an ancestral cystatin. Structurally it consists of a four-stranded anti-parallel beta-sheet gripping an alpha-helix in much the same manner that a hand grips a tennis racket. The unstructured N-terminus and the loop connecting beta-strands 1 and 2 are important for pepsin inhibition, but the loop connecting strands 3 and 4 is not.


Pssm-ID: 407092  Cd Length: 83  Bit Score: 83.63  E-value: 5.36e-21
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15238418    61 IDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVI-EAGEKKIYEAKVWVKPWMNFKQLQEFK 132
Cdd:pfam16845   9 VVEIAKFAVSEHNKQSKANLVFVKVVKGETQVVSGTNYRLVIAAKdGGGATKNYEAVVWEKPWEHFRSLTSFK 81
CY smart00043
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 45-132 1.81e-17

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 214484  Cd Length: 107  Bit Score: 75.16  E-value: 1.81e-17
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15238418     45 LGGFSDSK-NDwnggKEIDDIALFAVQEHNRRENAVLELA--RVLKATEQVVAGKLYRLTLEVIEAGEKK---------- 111
Cdd:smart00043   3 LGGPSDVPpND----PEVQEAADFAVAEYNKKSNDKYELRviKVVSAKSQVVAGTNYYLKVEVGETNCKKlsvdlencpf 78

                           90       100
                   ....*....|....*....|....*..
gi 15238418    112 ------IYEAKVWVKPWMNFKQLQEFK 132
Cdd:smart00043  79 ldqgekFCTAKVWEKPWENKIKLVEFK 105
CY cd00042
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 59-132 1.63e-16

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 238002 [Multi-domain]  Cd Length: 105  Bit Score: 72.51  E-value: 1.63e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15238418  59 KEIDDIALFAVQEHNRRENA-VLELARVLKATEQVVAGKLYRLTLEVIEA-------------------GEKKIYEAKVW 118
Cdd:cd00042  11 PEVQELADFAVAEYNKKSNDkYLEFFKVLSAKSQVVAGTNYYITVEAGDTnckkssvpldcpdcklleeGKKKFCTAKVW 90

                        90
                ....*....|....
gi 15238418 119 VKPWMNFKQLQEFK 132
Cdd:cd00042  91 EKPWENFKELLSFK 104
CY smart00043
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 154-214 5.59e-05

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 214484  Cd Length: 107  Bit Score: 41.26  E-value: 5.59e-05
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15238418    154 WRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPYKLIDIILARAKVVeERVKFELLLKLERGN 214
Cdd:smart00043   6 PSDVPPNDPEVQEAADFAVAEYNKKSNDKYELRVIKVVSAKSQVV-AGTNYYLKVEVGETN 65
CY cd00042
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 154-214 8.52e-05

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 238002 [Multi-domain]  Cd Length: 105  Bit Score: 40.54  E-value: 8.52e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15238418 154 WRSVSTNNPEVQEAAKHAMKSLQQKSNSLFpYKLIDIILARAKVVeERVKFELLLKLERGN 214
Cdd:cd00042   3 PSDIPANDPEVQELADFAVAEYNKKSNDKY-LEFFKVLSAKSQVV-AGTNYYITVEAGDTN 61
Cystatin pfam00031
Cystatin domain; Very diverse family. Attempts to define separate sub-families failed. ...
 157-198 5.35e-03

Cystatin domain; Very diverse family. Attempts to define separate sub-families failed. Typically, either the N-terminal or C-terminal end is very divergent. But splitting into two domains would make very short families. pfam00666 is related to this family but has not been included.


Pssm-ID: 425431  Cd Length: 92  Bit Score: 35.00  E-value: 5.35e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 15238418   157 VSTNNPEVQEAAKHAMKSLQQKSNSlFPYKLIDIILARAKVV 198
Cdd:pfam00031   6 IPDNSPELQEALDFALAEYNAKTDN-YYFELVEVKRAKSQVV 46
 
Name Accession Description Interval E-value
SQAPI pfam16845
Aspartic acid proteinase inhibitor; SQAPI, aspartic acid inhibitor first isolated from squash, ...
 61-132 5.36e-21

Aspartic acid proteinase inhibitor; SQAPI, aspartic acid inhibitor first isolated from squash, inhibits a wide range of aspartic proteinases. This particular family of PAAPIs (proteinaceous aspartic acid inhibitors) seems to have evolved quite recently from an ancestral cystatin. Structurally it consists of a four-stranded anti-parallel beta-sheet gripping an alpha-helix in much the same manner that a hand grips a tennis racket. The unstructured N-terminus and the loop connecting beta-strands 1 and 2 are important for pepsin inhibition, but the loop connecting strands 3 and 4 is not.


Pssm-ID: 407092  Cd Length: 83  Bit Score: 83.63  E-value: 5.36e-21
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15238418    61 IDDIALFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVI-EAGEKKIYEAKVWVKPWMNFKQLQEFK 132
Cdd:pfam16845   9 VVEIAKFAVSEHNKQSKANLVFVKVVKGETQVVSGTNYRLVIAAKdGGGATKNYEAVVWEKPWEHFRSLTSFK 81
CY smart00043
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 45-132 1.81e-17

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 214484  Cd Length: 107  Bit Score: 75.16  E-value: 1.81e-17
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15238418     45 LGGFSDSK-NDwnggKEIDDIALFAVQEHNRRENAVLELA--RVLKATEQVVAGKLYRLTLEVIEAGEKK---------- 111
Cdd:smart00043   3 LGGPSDVPpND----PEVQEAADFAVAEYNKKSNDKYELRviKVVSAKSQVVAGTNYYLKVEVGETNCKKlsvdlencpf 78

                           90       100
                   ....*....|....*....|....*..
gi 15238418    112 ------IYEAKVWVKPWMNFKQLQEFK 132
Cdd:smart00043  79 ldqgekFCTAKVWEKPWENKIKLVEFK 105
CY cd00042
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 59-132 1.63e-16

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 238002 [Multi-domain]  Cd Length: 105  Bit Score: 72.51  E-value: 1.63e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15238418  59 KEIDDIALFAVQEHNRRENA-VLELARVLKATEQVVAGKLYRLTLEVIEA-------------------GEKKIYEAKVW 118
Cdd:cd00042  11 PEVQELADFAVAEYNKKSNDkYLEFFKVLSAKSQVVAGTNYYITVEAGDTnckkssvpldcpdcklleeGKKKFCTAKVW 90

                        90
                ....*....|....
gi 15238418 119 VKPWMNFKQLQEFK 132
Cdd:cd00042  91 EKPWENFKELLSFK 104
Cystatin pfam00031
Cystatin domain; Very diverse family. Attempts to define separate sub-families failed. ...
 46-134 3.81e-12

Cystatin domain; Very diverse family. Attempts to define separate sub-families failed. Typically, either the N-terminal or C-terminal end is very divergent. But splitting into two domains would make very short families. pfam00666 is related to this family but has not been included.


Pssm-ID: 425431  Cd Length: 92  Bit Score: 60.43  E-value: 3.81e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15238418    46 GGFSDSKNDwngGKEIDDIALFAVQEHNRRE-NAVLELARVLKATEQVVAGKLYRLTLEVIEA---GEKKIYEAKVWV-K 120
Cdd:pfam00031   1 GGLSPIPDN---SPELQEALDFALAEYNAKTdNYYFELVEVKRAKSQVVAGTNYFIKVEVGETdcsKSKKLLEDCPLLdE 77

                          90
                  ....*....|....
gi 15238418   121 PWMNFKQLQEFKNI 134
Cdd:pfam00031  78 KWLGLCSAQVYKVP 91
CY smart00043
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 154-214 5.59e-05

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 214484  Cd Length: 107  Bit Score: 41.26  E-value: 5.59e-05
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15238418    154 WRSVSTNNPEVQEAAKHAMKSLQQKSNSLFPYKLIDIILARAKVVeERVKFELLLKLERGN 214
Cdd:smart00043   6 PSDVPPNDPEVQEAADFAVAEYNKKSNDKYELRVIKVVSAKSQVV-AGTNYYLKVEVGETN 65
CY cd00042
Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly ...
 154-214 8.52e-05

Cystatin-like domain; Cystatins are a family of cysteine protease inhibitors that occur mainly as single domain proteins. However some extracellular proteins such as kininogen, His-rich glycoprotein and fetuin also contain these domains.


Pssm-ID: 238002 [Multi-domain]  Cd Length: 105  Bit Score: 40.54  E-value: 8.52e-05
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15238418 154 WRSVSTNNPEVQEAAKHAMKSLQQKSNSLFpYKLIDIILARAKVVeERVKFELLLKLERGN 214
Cdd:cd00042   3 PSDIPANDPEVQELADFAVAEYNKKSNDKY-LEFFKVLSAKSQVV-AGTNYYITVEAGDTN 61
Cystatin pfam00031
Cystatin domain; Very diverse family. Attempts to define separate sub-families failed. ...
 157-198 5.35e-03

Cystatin domain; Very diverse family. Attempts to define separate sub-families failed. Typically, either the N-terminal or C-terminal end is very divergent. But splitting into two domains would make very short families. pfam00666 is related to this family but has not been included.


Pssm-ID: 425431  Cd Length: 92  Bit Score: 35.00  E-value: 5.35e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 15238418   157 VSTNNPEVQEAAKHAMKSLQQKSNSlFPYKLIDIILARAKVV 198
Cdd:pfam00031   6 IPDNSPELQEALDFALAEYNAKTDN-YYFELVEVKRAKSQVV 46
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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