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Conserved domains on  [gi|15240996|ref|NP_198691|]
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asparaginyl-tRNA synthetase family [Arabidopsis thaliana]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PLN02221 super family cl31844
asparaginyl-tRNA synthetase
 1-127 8.60e-36

asparaginyl-tRNA synthetase


The actual alignment was detected with superfamily member PLN02221:

Pssm-ID: 177867 [Multi-domain]  Cd Length: 572  Bit Score: 128.96  E-value: 8.60e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996    1 MGASSSTVQNLCSLSRTRIRSILCRPDGS--------------RISRELGK-SFTFLEVNYGSCAANLQV---------- 55
Cdd:PLN02221  17 LENDGSTVQKAQFSDRVLIRSILDRPDGGaglagqkvriggwvKTGREQGKgTFAFLEVNDGSCPANLQVmvdsslydls 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996   56 ---------------KIPLQGKATKEMIELNVEKFIDVGTVDhrsETPYSIPKSKLTLEYLRDHTYFRARTSMIAAVTRI 120
Cdd:PLN02221  97 tlvatgtcvtvdgvlKVPPEGKGTKQKIELSVEKVIDVGTVD---PTKYPLPKTKLTLEFLRDVLHLRSRTNSISAVARI 173


                 ....*..
gi 15240996  121 RHKMCLA 127
Cdd:PLN02221 174 RNALAFA 180
 
Name Accession Description Interval E-value
PLN02221 PLN02221
asparaginyl-tRNA synthetase
 1-127 8.60e-36

asparaginyl-tRNA synthetase


Pssm-ID: 177867 [Multi-domain]  Cd Length: 572  Bit Score: 128.96  E-value: 8.60e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996    1 MGASSSTVQNLCSLSRTRIRSILCRPDGS--------------RISRELGK-SFTFLEVNYGSCAANLQV---------- 55
Cdd:PLN02221  17 LENDGSTVQKAQFSDRVLIRSILDRPDGGaglagqkvriggwvKTGREQGKgTFAFLEVNDGSCPANLQVmvdsslydls 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996   56 ---------------KIPLQGKATKEMIELNVEKFIDVGTVDhrsETPYSIPKSKLTLEYLRDHTYFRARTSMIAAVTRI 120
Cdd:PLN02221  97 tlvatgtcvtvdgvlKVPPEGKGTKQKIELSVEKVIDVGTVD---PTKYPLPKTKLTLEFLRDVLHLRSRTNSISAVARI 173


                 ....*..
gi 15240996  121 RHKMCLA 127
Cdd:PLN02221 174 RNALAFA 180
AsnS COG0017
Aspartyl/asparaginyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; ...
 16-124 2.00e-12

Aspartyl/asparaginyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Aspartyl/asparaginyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439788 [Multi-domain]  Cd Length: 430  Bit Score: 62.76  E-value: 2.00e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996  16 RTRIRSILCRPDGSRIS--------RELGKsFTFLEVNYGSCaaNLQVKIPLQGKATKEMI-ELNVEKFIDV-GTVD--- 82
Cdd:COG0017   2 RTYIKDLLPEHVGQEVTvagwvrtkRDSGG-ISFLILRDGSG--FIQVVVKKDKLENFEEAkKLTTESSVEVtGTVVesp 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 15240996  83 ----------------HRSETPYSIPKSKLTLEYLRDHTYFRARTSMIAAVTRIRHKM 124
Cdd:COG0017  79 rapqgvelqaeeievlGEADEPYPLQPKRHSLEFLLDNRHLRLRTNRFGAIFRIRSEL 136
EcAsnRS_like_N cd04318
EcAsnRS_like_N: N-terminal, anticodon recognition domain of the type found in Escherichia coli ...
 37-72 4.44e-04

EcAsnRS_like_N: N-terminal, anticodon recognition domain of the type found in Escherichia coli asparaginyl-tRNA synthetase (AsnRS) and, in Arabidopsis thaliana and Saccharomyces cerevisiae mitochondrial (mt) AsnRS. This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. The enzymes in this group are homodimeric class2b aminoacyl-tRNA synthetases (aaRSs). aaRSs catalyze the specific attachment of amino acids (AAs) to their cognate tRNAs during protein biosynthesis. This 2-step reaction involves i) the activation of the AA by ATP in the presence of magnesium ions, followed by ii) the transfer of the activated AA to the terminal ribose of tRNA. In the case of the class2b aaRSs, the activated AA is attached to the 3'OH of the terminal ribose. Eukaryotes contain 2 sets of aaRSs, both of which are encoded by the nuclear genome. One set concerns with cytoplasmic protein synthesis, whereas the other exclusively with mitochondrial protein synthesis. S. cerevisiae mtAsnRS can charge E.coli tRNA with asparagines. Mutations in the gene for S. cerevisiae mtAsnRS has been found to induce a "petite" phenotype typical for a mutation in a nuclear gene that results in a non-functioning mitochondrial protein synthesis system.


Pssm-ID: 239813 [Multi-domain]  Cd Length: 82  Bit Score: 36.77  E-value: 4.44e-04
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 15240996  37 KSFTFLEVNYGSCAANLQVKIPLQGKATKEMIELNV 72
Cdd:cd04318  15 KKISFIELNDGSCLKNLQVVVDKELTNFKEILKLST 50
 
Name Accession Description Interval E-value
PLN02221 PLN02221
asparaginyl-tRNA synthetase
 1-127 8.60e-36

asparaginyl-tRNA synthetase


Pssm-ID: 177867 [Multi-domain]  Cd Length: 572  Bit Score: 128.96  E-value: 8.60e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996    1 MGASSSTVQNLCSLSRTRIRSILCRPDGS--------------RISRELGK-SFTFLEVNYGSCAANLQV---------- 55
Cdd:PLN02221  17 LENDGSTVQKAQFSDRVLIRSILDRPDGGaglagqkvriggwvKTGREQGKgTFAFLEVNDGSCPANLQVmvdsslydls 96

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996   56 ---------------KIPLQGKATKEMIELNVEKFIDVGTVDhrsETPYSIPKSKLTLEYLRDHTYFRARTSMIAAVTRI 120
Cdd:PLN02221  97 tlvatgtcvtvdgvlKVPPEGKGTKQKIELSVEKVIDVGTVD---PTKYPLPKTKLTLEFLRDVLHLRSRTNSISAVARI 173


                 ....*..
gi 15240996  121 RHKMCLA 127
Cdd:PLN02221 174 RNALAFA 180
asnC PRK03932
asparaginyl-tRNA synthetase; Validated
 15-124 3.12e-13

asparaginyl-tRNA synthetase; Validated


Pssm-ID: 235176 [Multi-domain]  Cd Length: 450  Bit Score: 65.13  E-value: 3.12e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996   15 SRTRIRSILCRP-DGSRIS--------RELGKsFTFLEVNYGSCAANLQVKIPLQGKATKEMIELNVEKFIDV-GTV--D 82
Cdd:PRK03932   2 MRVSIKDILKGKyVGQEVTvrgwvrtkRDSGK-IAFLQLRDGSCFKQLQVVKDNGEEYFEEIKKLTTGSSVIVtGTVveS 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 15240996   83 HRSETP-----------------YSIPKSKLTLEYLRDHTYFRARTSMIAAVTRIRHKM 124
Cdd:PRK03932  81 PRAGQGyelqatkievigedpedYPIQKKRHSIEFLREIAHLRPRTNKFGAVMRIRNTL 139
PLN02532 PLN02532
asparagine-tRNA synthetase
 41-130 4.03e-13

asparagine-tRNA synthetase


Pssm-ID: 215291 [Multi-domain]  Cd Length: 633  Bit Score: 64.89  E-value: 4.03e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996   41 FLEVNYGSCAANLQVKI------PLQGKAT------------------KEMIELNVEKFIDVGTVDHRSetpYSIPKSKL 96
Cdd:PLN02532 137 YLLISDGSCVASLQVVVdsalapLTQLMATgtcilaegvlklplpaqgKHVIELEVEKILHIGTVDPEK---YPLSKKRL 213

                         90       100       110
                 ....*....|....*....|....*....|....
gi 15240996   97 TLEYLRDHTYFRARTSMIAAVTRIRHKMCLAAKN 130
Cdd:PLN02532 214 PLDMLRDFSHFRPRTTTVASVTRVRSALTHATHT 247
AsnS COG0017
Aspartyl/asparaginyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; ...
 16-124 2.00e-12

Aspartyl/asparaginyl-tRNA synthetase [Translation, ribosomal structure and biogenesis]; Aspartyl/asparaginyl-tRNA synthetase is part of the Pathway/BioSystem: Aminoacyl-tRNA synthetases


Pssm-ID: 439788 [Multi-domain]  Cd Length: 430  Bit Score: 62.76  E-value: 2.00e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996  16 RTRIRSILCRPDGSRIS--------RELGKsFTFLEVNYGSCaaNLQVKIPLQGKATKEMI-ELNVEKFIDV-GTVD--- 82
Cdd:COG0017   2 RTYIKDLLPEHVGQEVTvagwvrtkRDSGG-ISFLILRDGSG--FIQVVVKKDKLENFEEAkKLTTESSVEVtGTVVesp 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 15240996  83 ----------------HRSETPYSIPKSKLTLEYLRDHTYFRARTSMIAAVTRIRHKM 124
Cdd:COG0017  79 rapqgvelqaeeievlGEADEPYPLQPKRHSLEFLLDNRHLRLRTNRFGAIFRIRSEL 136
PLN02603 PLN02603
asparaginyl-tRNA synthetase
 37-127 8.55e-12

asparaginyl-tRNA synthetase


Pssm-ID: 178213 [Multi-domain]  Cd Length: 565  Bit Score: 61.14  E-value: 8.55e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15240996   37 KSFTFLEVNYGSCAANLQ---------------------VKIPLQG-----KATKEMIELNVEKFIDVGtvdhRSETPYS 90
Cdd:PLN02603 123 SSVTFIEVNDGSCLSNMQcvmtpdaegydqvesglittgASVLVQGtvvssQGGKQKVELKVSKIVVVG----KSDPSYP 198

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 15240996   91 IPKSKLTLEYLRDHTYFRARTSMIAAVTRIRHKMCLA 127
Cdd:PLN02603 199 IQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYA 235
EcAsnRS_like_N cd04318
EcAsnRS_like_N: N-terminal, anticodon recognition domain of the type found in Escherichia coli ...
 37-72 4.44e-04

EcAsnRS_like_N: N-terminal, anticodon recognition domain of the type found in Escherichia coli asparaginyl-tRNA synthetase (AsnRS) and, in Arabidopsis thaliana and Saccharomyces cerevisiae mitochondrial (mt) AsnRS. This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. The enzymes in this group are homodimeric class2b aminoacyl-tRNA synthetases (aaRSs). aaRSs catalyze the specific attachment of amino acids (AAs) to their cognate tRNAs during protein biosynthesis. This 2-step reaction involves i) the activation of the AA by ATP in the presence of magnesium ions, followed by ii) the transfer of the activated AA to the terminal ribose of tRNA. In the case of the class2b aaRSs, the activated AA is attached to the 3'OH of the terminal ribose. Eukaryotes contain 2 sets of aaRSs, both of which are encoded by the nuclear genome. One set concerns with cytoplasmic protein synthesis, whereas the other exclusively with mitochondrial protein synthesis. S. cerevisiae mtAsnRS can charge E.coli tRNA with asparagines. Mutations in the gene for S. cerevisiae mtAsnRS has been found to induce a "petite" phenotype typical for a mutation in a nuclear gene that results in a non-functioning mitochondrial protein synthesis system.


Pssm-ID: 239813 [Multi-domain]  Cd Length: 82  Bit Score: 36.77  E-value: 4.44e-04
                        10        20        30
                ....*....|....*....|....*....|....*.
gi 15240996  37 KSFTFLEVNYGSCAANLQVKIPLQGKATKEMIELNV 72
Cdd:cd04318  15 KKISFIELNDGSCLKNLQVVVDKELTNFKEILKLST 50
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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