|
Name |
Accession |
Description |
Interval |
E-value |
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
6-649 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1325.53 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 6 LYPVHPEAVARTFTDEATYKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVkqtsFDDHHVDIKWFADGTLNVSHNCLDRH 85
Cdd:PRK00174 1 VFPPPAEFAANALIDMEQYKALYQESVEDPEGFWAEQAKRLDWFKPFDTV----LDWNAPFIKWFEDGELNVSYNCLDRH 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 86 LAERGDQVAIIWEGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGG 165
Cdd:PRK00174 77 LKTRGDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVVFGG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 166 FSPEALAGRIIDCKSKVVITADEGVRGGKRTPLKANVDDALTNPEtsSVQKIIVCKRTGAEIKWNQHRDVWYDDLMKVAG 245
Cdd:PRK00174 157 FSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALANCP--SVEKVIVVRRTGGDVDWVEGRDLWWHELVAGAS 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 246 STCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGA 325
Cdd:PRK00174 235 DECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHSYIVYGPLANGA 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 326 TTILFEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQS 405
Cdd:PRK00174 315 TTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEAWEWYYKVVGGE 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 406 RCPIVDTWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFV 485
Cdd:PRK00174 395 RCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMMRTIYGDHERFV 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 486 DTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTL 565
Cdd:PRK00174 475 KTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDIKGQGIYAFVTL 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 566 NSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEYdTLGDISTLADPGVVQHLIETH 645
Cdd:PRK00174 555 KGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGEE-ILGDTSTLADPSVVEKLIEAR 633
|
....
gi 15596084 646 RSMQ 649
Cdd:PRK00174 634 QNRK 637
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
23-644 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1152.76 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 23 TYKTMYQQSVVNPDGFWREQAQR-IDWIKPFEKVKQTSFDDhhvDIKWFADGTLNVSHNCLDRHLAERGDQVAIIWEGDD 101
Cdd:TIGR02188 6 QYKELYEESIEDPDKFWAKLARElLDWFKPFTKVLDWSFPP---FYKWFVGGELNVSYNCVDRHLEARPDKVAIIWEGDE 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 102 PADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSK 181
Cdd:TIGR02188 83 PGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEALADRINDAGAK 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 182 VVITADEGVRGGKRTPLKANVDDALTNPETSsVQKIIVCKRTGAEI-KWNQHRDVWYDDLMKVAGSTCAPKEMGAEDPLF 260
Cdd:TIGR02188 163 LVITADEGLRGGKVIPLKAIVDEALEKCPVS-VEHVLVVRRTGNPVvPWVEGRDVWWHDLMAKASAYCEPEPMDSEDPLF 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 261 ILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDVT 340
Cdd:TIGR02188 242 ILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLANGATTVMFEGVPTYPDPG 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 341 RVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPIVDTWWQTETGA 420
Cdd:TIGR02188 322 RFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVVGKERCPIVDTWWQTETGG 401
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 421 CLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGN-LVILDSWPGQARTLFGDHDRFVDTYFKTFKGMYFTG 499
Cdd:TIGR02188 402 IMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGPGEGGyLVIKQPWPGMLRTIYGDHERFVDTYFSPFPGYYFTG 481
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 500 DGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQEL 579
Cdd:TIGR02188 482 DGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYAFVTLKDGYEPDDELRKEL 561
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 580 KQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEYDTLGDISTLADPGVVQHLIET 644
Cdd:TIGR02188 562 RKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEELIEA 626
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
23-634 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1136.90 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 23 TYKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVKQTSFDdhHVDIKWFADGTLNVSHNCLDRHLAERGDQVAIIWEGDDP 102
Cdd:cd05966 2 QYKELYKQSIEDPEEFWGEIAKELDWFKPWDKVLDWSKG--PPFIKWFEGGKLNISYNCLDRHLKERGDKVAIIWEGDEP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 103 ADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd05966 80 DQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRINDAQCKL 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 183 VITADEGVRGGKRTPLKANVDDALTnpETSSVQKIIVCKRTGAEIKWNQHRDVWYDDLMKVAGSTCAPKEMGAEDPLFIL 262
Cdd:cd05966 160 VITADGGYRGGKVIPLKEIVDEALE--KCPSVEKVLVVKRTGGEVPMTEGRDLWWHDLMAKQSPECEPEWMDSEDPLFIL 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 263 YTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDVTRV 342
Cdd:cd05966 238 YTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFEGTPTYPDPGRY 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 343 AKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPIVDTWWQTETGACL 422
Cdd:cd05966 318 WDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVDTWWQTETGGIM 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 423 MTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFVDTYFKTFKGMYFTGDGA 502
Cdd:cd05966 398 ITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSKFPGYYFTGDGA 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 503 RRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQW 582
Cdd:cd05966 478 RRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEPSDELRKELRKH 557
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|..
gi 15596084 583 VRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEyDTLGDISTLAD 634
Cdd:cd05966 558 VRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGE-EELGDTSTLAD 608
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
68-643 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 930.68 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 68 KWFADGTLNVSHNCLDRHLAERGDQVAIIWEGDDpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVV 147
Cdd:COG0365 1 RWFVGGRLNIAYNCLDRHAEGRGDKVALIWEGED-GEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 148 AMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVRGGKRTPLKANVDDALtnPETSSVQKIIVCKRTGAEI 227
Cdd:COG0365 80 AMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEAL--EELPSLEHVIVVGRTGADV 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 228 KWnqHRDVWYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADI 307
Cdd:COG0365 158 PM--EGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVFWCTADI 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 308 GWVTGHTYIVYGPLANGATTILFEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVG 387
Cdd:COG0365 236 GWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSLRLLGSAG 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 388 EPINPEAWQWYYETVGqsrCPIVDTWWQTETGACLMTPLPGaHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIL 467
Cdd:COG0365 316 EPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEEGELVIK 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 DSWPGQARTLFGDHDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAV 547
Cdd:COG0365 392 GPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPAVAEAAV 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 548 VGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEydTLG 627
Cdd:COG0365 472 VGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAEGR--PLG 549
|
570
....*....|....*.
gi 15596084 628 DISTLADPGVVQHLIE 643
Cdd:COG0365 550 DTSTLEDPEALDEIKE 565
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
24-611 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 848.79 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 24 YKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVKQTSFDDHHVDIKWFADGTLNVSHNCLDRHLAERGDQVAIIWEGDDPA 103
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWITPYQKVKNTSFAPGAPSIKWFEDATLNLAANALDRHLRENGDRTAIIYEGDDTS 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd17634 81 QSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDSSSRLL 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITADEGVRGGKRTPLKANVDDALtNPETSSVQKIIVCKRTGAEIKWNQHRDVWYDDLMKVAGSTCAPKEMGAEDPLFILY 263
Cdd:cd17634 161 ITADGGVRAGRSVPLKKNVDDAL-NPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIAKASPEHQPEAMNAEDPLFILY 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDVTRVA 343
Cdd:cd17634 240 TSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLYEGVPNWPTPARMW 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 344 KIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPIVDTWWQTETGACLM 423
Cdd:cd17634 320 QVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVVDTWWQTETGGFMI 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 424 TPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFVDTYFKTFKGMYFTGDGAR 503
Cdd:cd17634 400 TPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFSTFKGMYFSGDGAR 479
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWV 583
Cdd:cd17634 480 RDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVEPSPELYAELRNWV 559
|
570 580
....*....|....*....|....*...
gi 15596084 584 RREIGPIATPDVIQWAPGLPKTRSGKIM 611
Cdd:cd17634 560 RKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
24-643 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 826.46 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 24 YKTMYQQSVVNPDGFWREQAQRIDWIKPFE--KVKQTSFD--DHHVDIKWFADGTLNVSHNCLDRHL-AERGDQVAIIWE 98
Cdd:PLN02654 32 YMEMYKRSVDDPAGFWSDIASQFYWKQKWEgdEVCSENLDvrKGPISIEWFKGGKTNICYNCLDRNVeAGNGDKIAIYWE 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 99 GDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDC 178
Cdd:PLN02654 112 GNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSAESLAQRIVDC 191
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 179 KSKVVITADEGVRGGKRTPLKANVDDALTNPETSSVQkIIVC---------KRTGAeiKWNQHRDVWYDDLMKVAGSTCA 249
Cdd:PLN02654 192 KPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVS-VGICltyenqlamKREDT--KWQEGRDVWWQDVVPNYPTKCE 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 250 PKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTIL 329
Cdd:PLN02654 269 VEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVYWCTADCGWITGHSYVTYGPMLNGATVLV 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 330 FEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPI 409
Cdd:PLN02654 349 FEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRCPI 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 410 VDTWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFVDTYF 489
Cdd:PLN02654 429 SDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECSGYLCVKKSWPGAFRTLYGDHERYETTYF 508
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 490 KTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGV 569
Cdd:PLN02654 509 KPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGIEHEVKGQGIYAFVTLVEGV 588
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15596084 570 EPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEYDTLGDISTLADPGVVQHLIE 643
Cdd:PLN02654 589 PYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGDTSTLADPGVVDQLIA 662
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
24-641 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 647.84 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 24 YKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVKQtsfDDHHVDIKWFADGTLNVSHNCLDRH-LAERGDQVAIIWEGDDP 102
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFKPPEKILD---NSNPPFTRWFVGGRLNTCYNALDRHvEAGRGDQIALIYDSPVT 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 103 ADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd05967 78 GTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDAKPKL 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 183 VITADEGVRGGKRTPLKANVDDALtnpETSSVQ--KIIVCKRTGAEIKWNQH-RDVWYDDLMKVAGST-CAPKEmgAEDP 258
Cdd:cd05967 158 IVTASCGIEPGKVVPYKPLLDKAL---ELSGHKphHVLVLNRPQVPADLTKPgRDLDWSELLAKAEPVdCVPVA--ATDP 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 259 LFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVP-NYP 337
Cdd:cd05967 233 LYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEGKPvGTP 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 338 DVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAA--VAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQ 415
Cdd:cd05967 313 DPGAFWRVIEKYQVNALFTAPTAIRAIRKEDPDGkyIKKYDLSSLRTLFLAGERLDPPTLEWAENTLGV---PVIDHWWQ 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 416 TETGACLMTPLPG--AHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVI-LDSWPGQARTLFGDHDRFVDTYFKTF 492
Cdd:cd05967 390 TETGWPITANPVGlePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIkLPLPPGCLLTLWKNDERFKKLYLSKF 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 493 KGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPS 572
Cdd:cd05967 470 PGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLKEGVKIT 549
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 573 -EALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEydTLGDISTLADPGVVQHL 641
Cdd:cd05967 550 aEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIADGE--DYTIPSTIEDPSVLDEI 617
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
22-639 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 606.18 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 22 ATYKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVKQTS---FddhhvdIKWFADGTLNVSHNCLDRHLAERGDQVAIIWE 98
Cdd:PRK10524 2 MSYSEFYQRSIDDPEAFWAEQARRIDWQTPFTQVLDYSnppF------ARWFVGGRTNLCHNAVDRHLAKRPEQLALIAV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 99 GDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDC 178
Cdd:PRK10524 76 STETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDA 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 179 KSKVVITADEGVRGGKRTPLKANVDDALTNPETSSvQKIIVCKRTGAEIKWNQHRDVWYDDLMKVAGSTCAPKE-MGAED 257
Cdd:PRK10524 156 KPVLIVSADAGSRGGKVVPYKPLLDEAIALAQHKP-RHVLLVDRGLAPMARVAGRDVDYATLRAQHLGARVPVEwLESNE 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 258 PLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYP 337
Cdd:PRK10524 235 PSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMYEGLPTRP 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 338 DVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQTE 417
Cdd:PRK10524 315 DAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALGV---PVIDNYWQTE 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 418 TGACLMTPLPGAHAM--KPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDS--WPGQARTLFGDHDRFVDTYFKTFK 493
Cdd:PRK10524 392 TGWPILAIARGVEDRptRLGSPGVPMYGYNVKLLNEVTGEPCGPNEKGVLVIEGplPPGCMQTVWGDDDRFVKTYWSLFG 471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 494 GM-YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGV--- 569
Cdd:PRK10524 472 RQvYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPKDSDsla 551
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 570 --EPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRkiAAAEYDTLGDISTLADPGVVQ 639
Cdd:PRK10524 552 drEARLALEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQ--AIAEGRDPGDLTTIEDPAALQ 621
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
22-635 |
0e+00 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 535.15 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 22 ATYKTMYQQSVVNPDGFWREQAQRID--WIKPFEKVKQTSfdDHHVDIKWFADGTLNVSHNCLDRHLAERGDQVAIIWEG 99
Cdd:cd05968 7 PDLEAFLERSAEDNAWFWGEFVKDVGieWYEPPYQTLDLS--GGKPWAAWFVGGRMNIVEQLLDKWLADTRTRPALRWEG 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 100 DDPADhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCK 179
Cdd:cd05968 85 EDGTS-RTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQDAE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 180 SKVVITADEGVRGGKRTPLKANVDDALTnpETSSVQKIIVCKRTGAEIKWNQHRDVWYDDLMKVAGSTCApkEMGAEDPL 259
Cdd:cd05968 164 AKALITADGFTRRGREVNLKEEADKACA--QCPTVEKVVVVRHLGNDFTPAKGRDLSYDEEKETAGDGAE--RTESEDPL 239
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 260 FILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGE-VYWCTaDIGWVTGhTYIVYGPLANGATTILFEGVPNYPD 338
Cdd:cd05968 240 MIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATMVLYDGAPDHPK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 339 VTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPIVDTWWQTET 418
Cdd:cd05968 318 ADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRNPIINYSGGTEI 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 419 -----GACLMTPLpgahamKPGSAAKPFFGVVPALVDNLGNLIEGAaEGNLVILDSWPGQARTLFGDHDRFVDTYFKTFK 493
Cdd:cd05968 398 sggilGNVLIKPI------KPSSFNGPVPGMKADVLDESGKPARPE-VGELVLLAPWPGMTRGFWRDEDRYLETYWSRFD 470
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 494 GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSE 573
Cdd:cd05968 471 NVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFVVLKPGVTPTE 550
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 574 ALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRkiAAAEYDTLGDISTLADP 635
Cdd:cd05968 551 ALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIR--AAYLGKELGDLSSLENP 610
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
48-634 |
2.10e-172 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 503.27 E-value: 2.10e-172
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 48 WIKPFEKVKQT-SFDDHHVDIKWFADGTLNVSHNCLDRHLA-ERGDQVAIIWEGDDPadHQEITYRQLHEQVCKFANALR 125
Cdd:PRK04319 14 NLKDYEETYATfSWEEVEKEFSWLETGKVNIAYEAIDRHADgGRKDKVALRYLDASR--KEKYTYKELKELSNKFANVLK 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 126 GQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVRggkRTPLkanvdda 205
Cdd:PRK04319 92 ELGVEKGDRVFIFMPRIPELYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALLE---RKPA------- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 206 ltnPETSSVQKIIVCKRTGAEIKwnqhRDVWYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGgylvyA 285
Cdd:PRK04319 162 ---DDLPSLKHVLLVGEDVEEGP----GTLDFNALMEQASDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHN-----A 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 286 SLTH----ERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGvpnYPDVTRVAKIIDKHKVNILYTAPTAI 361
Cdd:PRK04319 230 MLQHyqtgKYVLDLHEDDVYWCTADPGWVTGTSYGIFAPWLNGATNVIDGG---RFSPERWYRILEDYKVTVWYTAPTAI 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 362 RAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQTETGACLMTPLPgAHAMKPGSAAKPF 441
Cdd:PRK04319 307 RMLMGAGDDLVKKYDLSSLRHILSVGEPLNPEVVRWGMKVFGL---PIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPL 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 442 FGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFvDTYFKtfKGMYFTGDGARRDEDGYYWITGRVDDVLN 521
Cdd:PRK04319 383 PGIEAAIVDDQGNELPPNRMGNLAIKKGWPSMMRGIWNNPEKY-ESYFA--GDWYVSGDSAYMDEDGYFWFQGRVDDVIK 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 522 VSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPG 601
Cdd:PRK04319 460 TSGERVGPFEVESKLMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDK 539
|
570 580 590
....*....|....*....|....*....|....
gi 15596084 602 LPKTRSGKIMRRILRkiaAAEYD-TLGDISTLAD 634
Cdd:PRK04319 540 LPKTRSGKIMRRVLK---AWELGlPEGDLSTMED 570
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
109-619 |
6.33e-140 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 415.75 E-value: 6.33e-140
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:cd05969 2 TFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTEE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 189 GVRggKRTPlkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgaEDPLFILYTSGST 268
Cdd:cd05969 82 LYE--RTDP----------------------------------------------------------EDPTLLHYTSGTT 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 269 GKPKGVLHTTGGYLVYAsLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGvpnYPDVTRVAKIIDK 348
Cdd:cd05969 102 GTPKGVLHVHDAMIFYY-FTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEG---RFDAESWYGIIER 177
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 349 HKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQTETGACLMTPLPG 428
Cdd:cd05969 178 VKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFGV---PIHDTWWQTETGSIMIANYPC 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 429 AHAmKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFvDTYFKTfkGMYFTGDGARRDEDG 508
Cdd:cd05969 255 MPI-KPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERY-KNSFID--GWYLTGDLAYRDEDG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 509 YYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIG 588
Cdd:cd05969 331 YFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIINFVRQKLG 410
|
490 500 510
....*....|....*....|....*....|.
gi 15596084 589 PIATPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:cd05969 411 AHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
109-617 |
2.89e-117 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 356.65 E-value: 2.89e-117
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITade 188
Cdd:cd05972 2 SFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT--- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 189 gvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemGAEDPLFILYTSGST 268
Cdd:cd05972 79 -----------------------------------------------------------------DAEDPALIYFTSGTT 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 269 GKPKGVLHTTG---GYLVYASLtherVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDvtRVAKI 345
Cdd:cd05972 94 GLPKGVLHTHSyplGHIPTAAY----WLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRFDAE--RILEL 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 346 IDKHKVNILYTAPTAIRAMMAEGkaaVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQTETGACLMTp 425
Cdd:cd05972 168 LERYGVTSFCGPPTAYRMLIKQD---LSSYKFSHLRLVVSAGEPLNPEVIEWWRAATGL---PIRDGYGQTETGLTVGN- 240
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 426 LPGAhAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDR----FVDTYfktfkgmYFTGDG 501
Cdd:cd05972 241 FPDM-PVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKteasIRGDY-------YLTGDR 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 502 ARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQ 581
Cdd:cd05972 313 AYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQG 392
|
490 500 510
....*....|....*....|....*....|....*.
gi 15596084 582 WVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05972 393 HVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
82-523 |
2.88e-105 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 325.42 E-value: 2.88e-105
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIiwegdDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:pfam00501 1 LERQAARTPDKTAL-----EVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVP 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADEgvrggkrtPLKANVDDALTNPEtsSVQKIIVCKRTGAEIKWNQHRDVWYDDLm 241
Cdd:pfam00501 76 LNPRLPAEELAYILEDSGAKVLITDDA--------LKLEELLEALGKLE--VVKLVLVLDRDPVLKEEPLPEEAKPADV- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 kvagSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTgGYLVYASLTH----ERVFDYRPGEVYWCTADIGWVTGHTYIV 317
Cdd:pfam00501 145 ----PPPPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIkrvrPRGFGLGPDDRVLSTLPLFHDFGLSLGL 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 YGPLANGATTILFEGVPNyPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGkaAVAGADGSSLRLLGSVGEPINPEAWQW 397
Cdd:pfam00501 220 LGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAG--APKRALLSSLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 398 YYETVGqsrCPIVDTWWQTETGACLMTPLPGAHAM-KPGSAAKPFFGVVPALVD-NLGNLIEGAAEGNLVIldSWPGQAR 475
Cdd:pfam00501 297 FRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDdETGEPVPPGEPGELCV--RGPGVMK 371
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 15596084 476 TLFGDHDRFVDTYFKtfKGMYFTGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:pfam00501 372 GYLNDPELTAEAFDE--DGWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
82-621 |
5.73e-96 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 302.50 E-value: 5.73e-96
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:COG0318 5 LRRAAARHPDRPALVFGG------RRLTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVP 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITAdegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlm 241
Cdd:COG0318 79 LNPRLTAEELAYILEDSGARALVTA------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 kvagstcapkemgaedplFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPL 321
Cdd:COG0318 104 ------------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPL 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 ANGATTILFEGVpnypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINPEAWQWYYET 401
Cdd:COG0318 165 LAGATLVLLPRF----DPERVLELIERERVTVLFGVPTMLARLLRH--PEFARYDLSSLRLVVSGGAPLPPELLERFEER 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 402 VGqsrCPIVDTWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDH 481
Cdd:COG0318 239 FG---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELPPGEVGEIVV--RGPNVMKGYWNDP 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 482 DRFVdtyfKTFK-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIY 560
Cdd:COG0318 314 EATA----EAFRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGERVV 389
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15596084 561 VYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:COG0318 390 AFVVLRPGAELDAE---ELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAA 447
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
257-611 |
6.09e-92 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 288.03 E-value: 6.09e-92
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 257 DPLFILYTSGSTGKPKGVLHTTGGYLVYASLThERVFDYRPGEVYWCTADIGWVtGHTYIVYGPLANGATTILFEGvpny 336
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAAL-AASGGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK---- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 337 PDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQT 416
Cdd:cd04433 75 FDPEAALELIEREKVTILLGVPTLLARLLKA--PESAGYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 417 ETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldswpgQARTLF-GDHDRFVDTYFKTFKGM 495
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVV------RGPSVMkGYWNNPEATAAVDEDGW 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 496 YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAl 575
Cdd:cd04433 224 YRTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAE- 302
|
330 340 350
....*....|....*....|....*....|....*.
gi 15596084 576 rqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIM 611
Cdd:cd04433 303 --ELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
22-643 |
9.26e-91 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 295.17 E-value: 9.26e-91
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 22 ATYKTMYQQSVVNPDGFWREQAQ--RIDWIKPFEKVKQtsfDDHHVDIKWFADGTLNVSHNCLdRHlaERGDQVAIIWEG 99
Cdd:PRK03584 34 DDYAALWRWSVEDLEAFWQSVWDffGVIGSTPYTVVLA---GRRMPGARWFPGARLNYAENLL-RH--RRDDRPAIIFRG 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 100 DDpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCK 179
Cdd:PRK03584 108 ED-GPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQGVLDRFGQIE 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 180 SKVVITADeGVR-GGK---RTPLKANVDDALTnpetsSVQKIIVCKRTGAEIKWNQHRDV--WYDDLMKVAGSTCAPKEM 253
Cdd:PRK03584 187 PKVLIAVD-GYRyGGKafdRRAKVAELRAALP-----SLEHVVVVPYLGPAAAAAALPGAllWEDFLAPAEAAELEFEPV 260
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 254 GAEDPLFILYTSGSTGKPKGVLHTTGGYLvyasLTH--ERVF--DYRPGEVY-WCTAdIGW------VTGhtyivygpLA 322
Cdd:PRK03584 261 PFDHPLWILYSSGTTGLPKCIVHGHGGIL----LEHlkELGLhcDLGPGDRFfWYTT-CGWmmwnwlVSG--------LL 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 323 NGATTILFEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETV 402
Cdd:PRK03584 328 VGATLVLYDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDWVYEHV 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 403 GQsrcpivDTWWQTETG-----ACLMtplpGAHAMKP---GSAAKPFFGVVPALVDNLGNLIEGAAeGNLVILDSWPGQA 474
Cdd:PRK03584 408 KA------DVWLASISGgtdicSCFV----GGNPLLPvyrGEIQCRGLGMAVEAWDEDGRPVVGEV-GELVCTKPFPSMP 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFGDHD--RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQH 552
Cdd:PRK03584 477 LGFWNDPDgsRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEW 556
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 553 DIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIM----RRILRKiaaAEYDTLGD 628
Cdd:PRK03584 557 PDGDVRMPLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKVelpvKKLLHG---RPVKKAVN 633
|
650
....*....|....*
gi 15596084 629 ISTLADPGVVQHLIE 643
Cdd:PRK03584 634 RDALANPEALDWFAD 648
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
108-617 |
3.13e-85 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 274.01 E-value: 3.13e-85
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITad 187
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 188 egvrggkrtplkaNVDdaltnpetssvqkiivckrtgaeikwNQHRDvwyddlmkvagstcapkemgAEDPLFILYTSGS 267
Cdd:cd05973 79 -------------DAA--------------------------NRHKL--------------------DSDPFVMMFTSGT 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 268 TGKPKGVLHTT------GGYLVYAslthervFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDVTR 341
Cdd:cd05973 100 TGLPKGVPVPLralaafGAYLRDA-------VDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEGGFSVESTWR 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 342 vakIIDKHKVNILYTAPTAIRAMMAEGkAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETGAC 421
Cdd:cd05973 173 ---VIERLGVTNLAGSPTAYRLLMAAG-AEVPARPKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQTELGMV 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 422 LMTPLPGAHAMKPGSAAKPFFGV-VPALVDNLGNLIEGAAeGNLVIldswpgqartlfgDHDRFVDTYF--------KTF 492
Cdd:cd05973 246 LANHHALEHPVHAGSAGRAMPGWrVAVLDDDGDELGPGEP-GRLAI-------------DIANSPLMWFrgyqlpdtPAI 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 493 KGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEP 571
Cdd:cd05973 312 DGGYYlTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHEG 391
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 15596084 572 SEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05973 392 TPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
22-618 |
4.61e-84 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 276.84 E-value: 4.61e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 22 ATYKTMYQQSVVNPDGFWREQAQ--RIDWIKPFEKVKQTsfDDHHVDIKWFADGTLNVSHNCLdRHlAERGDQVAIIWEG 99
Cdd:cd05943 17 ADYAALHRWSVDDPGAFWAAVWDfsGVRGSKPYDVVVVS--GRIMPGARWFPGARLNYAENLL-RH-ADADDPAAIYAAE 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 100 DDPADhqEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCK 179
Cdd:cd05943 93 DGERT--EVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRFGQIE 170
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 180 SKVVITADEGVRGGKRTPLKANVddALTNPETSSVQKIIVCKRTGAEIKWN---QHRDVWYDDLmkVAGSTCAPKE---M 253
Cdd:cd05943 171 PKVLFAVDAYTYNGKRHDVREKV--AELVKGLPSLLAVVVVPYTVAAGQPDlskIAKALTLEDF--LATGAAGELEfepL 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 254 GAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHtYIVYGpLANGATTILFEGV 333
Cdd:cd05943 247 PFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWN-WLVSG-LAVGATIVLYDGS 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 334 PNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrcpivDTW 413
Cdd:cd05943 325 PFYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIKP------DVL 398
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 414 WQTETGA-------CLMTPLPGAHamkPGSAAKPFFGVVPALVDNLGNLIEGAAeGNLVILDSWPGQARTLFGDHD--RF 484
Cdd:cd05943 399 LASISGGtdiiscfVGGNPLLPVY---RGEIQCRGLGMAVEAFDEEGKPVWGEK-GELVCTKPFPSMPVGFWNDPDgsRY 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 485 VDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVT 564
Cdd:cd05943 475 RAAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERVILFVK 554
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....
gi 15596084 565 LNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:cd05943 555 LREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKI 608
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
82-616 |
1.19e-79 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 261.53 E-value: 1.19e-79
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAE-RGDQVAIIwegdDPADHqeITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHS 160
Cdd:cd05959 9 VDLNLNEgRGDKTAFI----DDAGS--LTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIRAGIVPV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 161 VVFGGFSPEALAGRIIDCKSKVVITADEgvrggkrtpLKANVDDALTNPETSSVQkIIVCKRTGAEIKwnqhrDVWYDDL 240
Cdd:cd05959 83 PVNTLLTPDDYAYYLEDSRARVVVVSGE---------LAPVLAAALTKSEHTLVV-LIVSGGAGPEAG-----ALLLAEL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 241 MKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGP 320
Cdd:cd05959 148 VAAEAEQLKPAATHADDPAFWLYSSGSTGRPKGVVHLHADIYWTAELYARNVLGIREDDVCFSAAKLFFAYGLGNSLTFP 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 321 LANGATTILFegvPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAegkAAVAGA-DGSSLRLLGSVGEPINPEAWQWYY 399
Cdd:cd05959 228 LSVGATTVLM---PERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLA---APNLPSrDLSSLRLCVSAGEALPAEVGERWK 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 400 ETVGqsrCPIVDTWWQTETGACLMTPLPGAhaMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFG 479
Cdd:cd05959 302 ARFG---LDILDGIGSTEMLHIFLSNRPGR--VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYV--RGPSSATMYWN 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 480 DHDrfvDTYfKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQG 558
Cdd:cd05959 375 NRD---KTR-DTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAAVVGVEDEDGLTK 450
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 559 IYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05959 451 PKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
103-616 |
7.08e-75 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 246.99 E-value: 7.08e-75
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 103 ADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:cd05919 6 AADRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARL 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 183 VITAdegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgAEDPLFIL 262
Cdd:cd05919 86 VVTS--------------------------------------------------------------------ADDIAYLL 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 263 YTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADI--GWVTGHTyiVYGPLANGATTILFegvPNYPDVT 340
Cdd:cd05919 98 YSSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVLN---PGWPTAE 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 341 RVAKIIDKHKVNILYTAPTAIRAMMAEGkaAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETGA 420
Cdd:cd05919 173 RVLATLARFRPTVLYGVPTFYANLLDSC--AGSPDALRSLRLCVSAGEALPRGLGERWMEHFG---GPILDGIGATEVGH 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 421 CLMTPLPGAhaMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFgdhdRFVDTYFKTFK-GMYFTG 499
Cdd:cd05919 248 IFLSNRPGA--WRLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLV--RGPSAAVGYW----NNPEKSRATFNgGWYRTG 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 500 DGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQEL 579
Cdd:cd05919 320 DKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLARDI 399
|
490 500 510
....*....|....*....|....*....|....*..
gi 15596084 580 KQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05919 400 HRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
17-617 |
1.53e-74 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 251.97 E-value: 1.53e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 17 TFTDEATYKTMYQQSVVNPDGFWREQAQR-IDWIKPFEKVkqTSFDDHHVDikWFADGTLNVSHNCLDRHL--AERGDQV 93
Cdd:PTZ00237 3 KLSDPFDYENDSNYANSNPESFWDEVAKKyVHWDKMYDKV--YSGDEIYPD--WFKGGELNTCYNVLDIHVknPLKRDQD 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 94 AIIWEGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAG 173
Cdd:PTZ00237 79 ALIYECPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSLID 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 174 RIIDCKSKVVITADEGVRGGKRTPLKANVDDALT--------------NPETSSVQKIIVckrtgAEIKWNQHRDVWYDD 239
Cdd:PTZ00237 159 RIETITPKLIITTNYGILNDEIITFTPNLKEAIElstfkpsnvitlfrNDITSESDLKKI-----ETIPTIPNTLSWYDE 233
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 240 LMKVAGSTCAP----KEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTY 315
Cdd:PTZ00237 234 IKKIKENNQSPfyeyVPVESSHPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVSFHGF 313
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 316 IvYGPLANGATTILFEG--VPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMA---EGKAAVAGADGSSLRLLGSVGEPI 390
Cdd:PTZ00237 314 L-YGSLSLGNTFVMFEGgiIKNKHIEDDLWNTIEKHKVTHTLTLPKTIRYLIKtdpEATIIRSKYDLSNLKEIWCGGEVI 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 391 NpEAWQWYYETVGQSRCPIVdtWWQTETGACLMtpLPGAHAMKPGSA-AKPFFGVVPALVDNLGNLIEGAAEGNLVI-LD 468
Cdd:PTZ00237 393 E-ESIPEYIENKLKIKSSRG--YGQTEIGITYL--YCYGHINIPYNAtGVPSIFIKPSILSEDGKELNVNEIGEVAFkLP 467
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 469 SWPGQARTLFGDHDRFvDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVV 548
Cdd:PTZ00237 468 MPPSFATTFYKNDEKF-KQLFSKFPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVLECCSI 546
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15596084 549 GMQH-DIKGQGIYVYVTLNSGVEPSEALRQ---ELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PTZ00237 547 GIYDpDCYNVPIGLLVLKQDQSNQSIDLNKlknEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQIISK 619
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
106-616 |
1.71e-74 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 245.80 E-value: 1.71e-74
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05971 5 EKVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 AdegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemGAEDPLFILYTS 265
Cdd:cd05971 85 D-------------------------------------------------------------------GSDDPALIIYTS 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLHTTG---GYLVYASLTHErvFDYRPGEVYWCTADIGWVtghtyivyGPLANGATTILFEGVPNYP----- 337
Cdd:cd05971 98 GTTGPPKGALHAHRvllGHLPGVQFPFN--LFPRDGDLYWTPADWAWI--------GGLLDVLLPSLYFGVPVLAhrmtk 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 338 -DVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQT 416
Cdd:cd05971 168 fDPKAALDLMSRYGVTTAFLPPTALKMMRQQGEQL--KHAQVKLRAIATGGESLGEELLGWAREQFG---VEVNEFYGQT 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 417 ETGAcLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQARTLFGDHDRFVdtyfKTFKGMY 496
Cdd:cd05971 243 ECNL-VIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDPVAFLGYWNNPSATE----KKMAGDW 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 497 F-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAL 575
Cdd:cd05971 318 LlTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDAL 397
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 15596084 576 RQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05971 398 AREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
60-616 |
3.56e-73 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 245.48 E-value: 3.56e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 60 FDDHHVDIKWFADGTLNVSHNCLDRHLAERGDQVAIIWeGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYM 139
Cdd:cd05970 1 YEDFHNNFSINVPENFNFAYDVVDAMAKEYPDKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTL 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 140 PMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVrggkrtpLKANVDDALtnPETSSVQKIIV 219
Cdd:cd05970 80 KRRYEFWYSLLALHKLGAIAIPATHQLTAKDIVYRIESADIKMIVAIAEDN-------IPEEIEKAA--PECPSKPKLVW 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 220 CkrtGAEIkwnqhRDVWYD---DLMKVAGS---TCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTG---GYLVYASLTHe 290
Cdd:cd05970 151 V---GDPV-----PEGWIDfrkLIKNASPDferPTANSYPCGEDILLVYFSSGTTGMPKMVEHDFTyplGHIVTAKYWQ- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 291 rvfDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDvtRVAKIIDKHKVNILYTAPTAIRAMMaegKA 370
Cdd:cd05970 222 ---NVREGGLHLTVADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDPK--ALLEKLSKYGVTTFCAPPTIYRFLI---RE 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 371 AVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDTWWQTETGACLMTpLPGAHAmKPGSAAKPFFGVVPALVD 450
Cdd:cd05970 294 DLSRYDLSSLRYCTTAGEALNPEVFNTFKEKTGIK---LMEGFGQTETTLTIAT-FPWMEP-KPGSMGKPAPGYEIDLID 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 451 NLGNLIEGAAEGNLVILDSwPGQARTLFGDHDRFVDTYFKTFK-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGT 529
Cdd:cd05970 369 REGRSCEAGEEGEIVIRTS-KGKPVGLFGGYYKDAEKTAEVWHdGYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGP 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 530 AEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGK 609
Cdd:cd05970 448 FEVESALIQHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGK 527
|
....*..
gi 15596084 610 IMRRILR 616
Cdd:cd05970 528 IRRVEIR 534
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
76-617 |
2.63e-67 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 229.27 E-value: 2.63e-67
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 76 NVSHNCLD-----RHLAERGDQVAIIWEgDDPADHQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAM 149
Cdd:cd05928 6 NFASDVLDqwadkEKAGKRPPNPALWWV-NGKGDEVKWSFRELGSLSRKAANVLSGAcGLQRGDRVAVILPRVPEWWLVN 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 150 LACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEgvrggkrtpLKANVDD-ALTNPETSSvqKIIVCkrtgaeik 228
Cdd:cd05928 85 VACIRTGLVFIPGTIQLTAKDILYRLQASKAKCIVTSDE---------LAPEVDSvASECPSLKT--KLLVS-------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 229 wNQHRDVWYD--DLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTAD 306
Cdd:cd05928 146 -EKSRDGWLNfkELLNEASTEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSD 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 307 IGWVTGHTYIVYGPLANGATTILFEgVPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGkaaVAGADGSSLRLLGSV 386
Cdd:cd05928 225 TGWIKSAWSSLFEPWIQGACVFVHH-LPRF-DPLVILKTLSSYPITTFCGAPTVYRMLVQQD---LSSYKFPSLQHCVTG 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 387 GEPINPEAWQWYYETVGQSrcpIVDTWWQTETGACLMTPlpGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVI 466
Cdd:cd05928 300 GEPLNPEVLEKWKAQTGLD---IYEGYGQTETGLICANF--KGMKIKPGSMGKASPPYDVQIIDDNGNVLPPGTEGDIGI 374
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 467 LDSwPGQARTLFGDHDRFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEA 545
Cdd:cd05928 375 RVK-PIRPFGLFSGYVDNPEKTAATIRGdFYLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVES 453
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15596084 546 AVVGMQHDIKGQGIYVYVTLNSGVEPS--EALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05928 454 AVVSSPDPIRGEVVKAFVVLAPQFLSHdpEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
72-616 |
1.92e-64 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 221.60 E-value: 1.92e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 72 DGTLNVSHnCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLA 151
Cdd:PRK06187 3 DYPLTIGR-ILRHGARKHPDKEAVYFDG------RRTTYAELDERVNRLANALRALGVKKGDRVAVFDWNSHEYLEAYFA 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 152 CTRIGAI-HSV-VFggFSPEALAGRIIDCKSKVVITADEGVrggkrtPLKANVDDALTnpetsSVQKIIVCKRTGAEIKW 229
Cdd:PRK06187 76 VPKIGAVlHPInIR--LKPEEIAYILNDAEDRVVLVDSEFV------PLLAAILPQLP-----TVRTVIVEGDGPAAPLA 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 230 NQHRDvwYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTtggylvYASLT-HERVfdyrpgevywCTADIG 308
Cdd:PRK06187 143 PEVGE--YEELLAAASDTFDFPDIDENDAAAMLYTSGTTGHPKGVVLS------HRNLFlHSLA----------VCAWLK 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 309 WVTGHTYIVYGPLAN-GATTI----LFEGVPN-YP---DVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAavAGADGSS 379
Cdd:PRK06187 205 LSRDDVYLVIVPMFHvHAWGLpylaLMAGAKQvIPrrfDPENLLDLIETERVTFFFAVPTIWQMLLKAPRA--YFVDFSS 282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 380 LRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETGACL-MTPLP---GAHAMKPGSAAKPFFGVVPALVDNLGNL 455
Cdd:PRK06187 283 LRLVIYGGAALPPALLREFKEKFG---IDLVQGYGMTETSPVVsVLPPEdqlPGQWTKRRSAGRPLPGVEARIVDDDGDE 359
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 456 IE--GAAEGNLVILDSWPGQArtLFGDHDRFVDTYfktFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVE 533
Cdd:PRK06187 360 LPpdGGEVGEIIVRGPWLMQG--YWNRPEATAETI---DGGWLHTGDVGYIDEDGYLYITDRIKDVIISGGENIYPRELE 434
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 534 SAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRR 613
Cdd:PRK06187 435 DALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAK---ELRAFLRGRLAKFKLPKRIAFVDELPRTSVGKILKR 511
|
...
gi 15596084 614 ILR 616
Cdd:PRK06187 512 VLR 514
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
82-616 |
1.28e-63 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 217.82 E-value: 1.28e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI--- 158
Cdd:cd05936 5 LEEAARRFPDKTALIFMG------RKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAVvvp 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGgfsPEALAGRIIDCKSKVVITadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrDVWYD 238
Cdd:cd05936 79 LNPLYT---PRELEHILNDSGAKALIV------------------------------------------------AVSFT 107
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 DLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFD-YRPGEVYWCTADIGWVTGHTYIV 317
Cdd:cd05936 108 DLLAAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRNLVANALQIKAWLEDlLEGDDVVLAALPLFHVFGLTVAL 187
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 YGPLANGATTILfegVPNyPDVTRVAKIIDKHKVNILYTAPTAIRAMMaeGKAAVAGADGSSLRLLGSVGEPINPEAWQW 397
Cdd:cd05936 188 LLPLALGATIVL---IPR-FRPIGVLKEIRKHRVTIFPGVPTMYIALL--NAPEFKKRDFSSLRLCISGGAPLPVEVAER 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 398 YYETVGqsrCPIVDTWWQTETG--ACLmTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVI------LDS 469
Cdd:cd05936 262 FEELTG---VPIVEGYGLTETSpvVAV-NPLDGPR--KPGSIGIPLPGTEVKIVDDDGEELPPGEVGELWVrgpqvmKGY 335
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 470 WPGQARTlfgdHDRFVDTYFKtfkgmyfTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd05936 336 WNRPEET----AEAFVDGWLR-------TGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAAVVG 404
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 550 MQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05936 405 VPDPYSGEAVKAFVVLKEGASLTE---EEIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
82-612 |
3.60e-63 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 215.55 E-value: 3.60e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:cd17631 1 LRRRARRHPDRTALVFGG------RSLTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVP 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVItadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlm 241
Cdd:cd17631 75 LNFRLTPPEVAYILADSGAKVLF--------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 kvagstcapkemgaEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPL 321
Cdd:cd17631 98 --------------DDLALLMYTSGTTGRPKGAMLTHRN-LLWNAVNALAALDLGPDDVLLVVAPLFHIGGLGVFTLPTL 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 ANGATTILFEGvpnyPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLGSVGEPINP---EAWQWY 398
Cdd:cd17631 163 LRGGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFA--TTDLSSLRAVIYGGAPMPErllRALQAR 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 399 YetvgqsrCPIVDTWWQTET--GACLMTPlpGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARt 476
Cdd:cd17631 237 G-------VKFVQGYGMTETspGVTFLSP--EDHRRKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVV--RGPHVMA- 304
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 477 lfGDHDRFVDTYfKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIK 555
Cdd:cd17631 305 --GYWNRPEATA-AAFRDGWFhTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKW 381
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 556 GQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17631 382 GEAVVAVVVPRPGAELDE---DELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
105-616 |
4.45e-59 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 204.63 E-value: 4.45e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 105 HQEITYRQLHEQVCKFANALRGQDVHR-GDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd05958 8 EREWTYRDLLALANRIANVLVGELGIVpGNRVLLRGSNSPELVACWFGIQKAGAIAVATMPLLRPKELAYILDKARITVA 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ItadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstCAPKEMGAEDPLFILY 263
Cdd:cd05958 88 L---------------------------------------------------------------CAHALTASDDICILAF 104
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGvpNYPDvtRVA 343
Cdd:cd05958 105 TSGTTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASGVLLEE--ATPD--LLL 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 344 KIIDKHKVNILYTAPTAIRAMMaeGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETGACLM 423
Cdd:cd05958 181 SAIARYKPTVLFTAPTAYRAML--AHPDAAGPDLSSLRKCVSAGEALPAALHRAWKEATG---IPIIDGIGSTEMFHIFI 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 424 TPLPGAhaMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHDRfvdTYFKtfKGMYFTGDGAR 503
Cdd:cd05958 256 SARPGD--ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAV--RGPTGCRYLADKRQR---TYVQ--GGWNITGDTYS 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWV 583
Cdd:cd05958 327 RDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRPGVIPGPVLARELQDHA 406
|
490 500 510
....*....|....*....|....*....|...
gi 15596084 584 RREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05958 407 KAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
22-617 |
5.56e-59 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 211.09 E-value: 5.56e-59
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 22 ATYKTMYQQSVVNPDGFWRE--QAQRIDWIKPFEKVKQTSFDDHHVDiKWFADGTLNVSHNCLDRHLAERGDQVAIIW-- 97
Cdd:PLN03052 120 SSFSEFQRFSVENPEVYWSIvlDELSLVFSVPPRCILDTSDESNPGG-QWLPGAVLNVAECCLTPKPSKTDDSIAIIWrd 198
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 98 EGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIID 177
Cdd:PLN03052 199 EGSDDLPVNRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRLKI 278
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 178 CKSKVVITADEGVRGGKRTPLKANVDDAlTNPetssvqKIIVCKRTGAEIKWN-QHRDVWYDDLMKVA-----GSTCAPK 251
Cdd:PLN03052 279 SKAKAIFTQDVIVRGGKSIPLYSRVVEA-KAP------KAIVLPADGKSVRVKlREGDMSWDDFLARAnglrrPDEYKAV 351
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 252 EMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYA--SLTHervFDYRPGEVY-WCTaDIGWVTGHtYIVYGPLANGATTI 328
Cdd:PLN03052 352 EQPVEAFTNILFSSGTTGEPKAIPWTQLTPLRAAadAWAH---LDIRKGDIVcWPT-NLGWMMGP-WLVYASLLNGATLA 426
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 329 LFEGVPNYPDvtrVAKIIDKHKVNILYTAPTAIRAMMAEGkaAVAGADGSSLRLLGSVGEPINPEAWQW-----YYEtvg 403
Cdd:PLN03052 427 LYNGSPLGRG---FAKFVQDAKVTMLGTVPSIVKTWKNTN--CMAGLDWSSIRCFGSTGEASSVDDYLWlmsraGYK--- 498
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 404 qsrcPIVDTWWQTETGACLMTplpgAHAMKP---GSAAKPFFGVVPALVDNLGNLI--EGAAEGNLVILDSWPGQARTLF 478
Cdd:PLN03052 499 ----PIIEYCGGTELGGGFVT----GSLLQPqafAAFSTPAMGCKLFILDDSGNPYpdDAPCTGELALFPLMFGASSTLL 570
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 479 -GDHDrfvDTYFK---TFKGMYFT--GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAM-VAHPKVAEAAVVGMQ 551
Cdd:PLN03052 571 nADHY---KVYFKgmpVFNGKILRrhGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAADESVLETAAIGVP 647
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 552 HdiKGQG------IYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PLN03052 648 P--PGGGpeqlviAAVLKDPPGSNPDLNELKKIFNSAIQKKLNPLFKVSAVVIVPSFPRTASNKVMRRVLRQ 717
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
91-613 |
1.23e-57 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 200.83 E-value: 1.23e-57
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVfggfSPEA 170
Cdd:cd05930 2 DAVAVVDGD------QSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPL----DPSY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 171 LAGRII----DCKSKVVITadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnQHRDVWYddlmkvags 246
Cdd:cd05930 72 PAERLAyileDSGAKLVLT---------------------------------------------DPDDLAY--------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 247 tcapkemgaedplfILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIvYGPLANGAT 326
Cdd:cd05930 98 --------------VIYTSGSTGKPKGVMVEHRG-LVNLLLWMQEAYPLTPGDRVLQFTSFSFDVSVWEI-FGALLAGAT 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 327 TILfegVPN--YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvagaDGSSLRLLGSVGEPINPEAWQWYYETVGQ 404
Cdd:cd05930 162 LVV---LPEevRKDPEALADLLAEEGITVLHLTPSLLRLLLQELELA----ALPSLRLVLVGGEALPPDLVRRWRELLPG 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 405 SRcpIVDTWWQTE-TGACLMTPLPGAHAmKPGSAA--KPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDH 481
Cdd:cd05930 235 AR--LVNLYGPTEaTVDATYYRVPPDDE-EDGRVPigRPIPNTRVYVLDENLRPVPPGVPGELYI--GGAGLARGYLNRP 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 482 D----RFVDTYFKTFKGMYFTGDGARRDEDG--YYwiTGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIK 555
Cdd:cd05930 310 EltaeRFVPNPFGPGERMYRTGDLVRWLPDGnlEF--LGRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGDG 387
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 556 GQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRR 613
Cdd:cd05930 388 EKRLVAYVVPDEGGELDEE---ELRAHLAERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
73-617 |
5.56e-55 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 195.44 E-value: 5.56e-55
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 73 GTLNVSHNCLDRHLAE-RGDQVAIIwegdDPAdhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLA 151
Cdd:TIGR02262 1 EKYNAAEDLLDRNVVEgRGGKTAFI----DDI--SSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLG 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 152 CTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEgvrggkrtpLKANVDDALTNPETSsVQKIIVCKRTGAEIKwnq 231
Cdd:TIGR02262 75 AIRAGIVPVALNTLLTADDYAYMLEDSRARVVFVSGA---------LLPVIKAALGKSPHL-EHRVVVGRPEAGEVQ--- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 232 hrdvwYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVT 311
Cdd:TIGR02262 142 -----LAELLATESEQFKPAATQADDPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDDVCFSAAKLFFAY 216
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 312 GHTYIVYGPLANGATTILFegvPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKaaVAGADGSSLRLLGSVGEPIN 391
Cdd:TIGR02262 217 GLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLADPN--LPSEDQVRLRLCTSAGEALP 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 392 PEAWQWYYETVGQSrcpIVDTWWQTETGACLMTPLPgaHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWP 471
Cdd:TIGR02262 292 AEVGQRWQARFGVD---IVDGIGSTEMLHIFLSNLP--GDVRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLI--SGP 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 472 GQARTLFGDHDRFVDTyfktFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGM 550
Cdd:TIGR02262 365 SSATMYWNNRAKSRDT----FQGEWTrSGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAVVGV 440
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 551 Q---HDIKGQGiyvYVTLNSGvepSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:TIGR02262 441 AdedGLIKPKA---FVVLRPG---QTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
108-619 |
6.36e-52 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 185.08 E-value: 6.36e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIiDCKSKVVITAD 187
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDRV-DRGGAVYAAVD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 188 EGVRggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgAEDPLFILYTSGS 267
Cdd:cd05974 80 ENTH---------------------------------------------------------------ADDPMLLYFTSGT 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 268 TGKPKGVLHTTGGYLVyASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFegvpNYP--DVTRVAKI 345
Cdd:cd05974 97 TSKPKLVEHTHRSYPV-GHLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATVFLF----NYArfDAKRVLAA 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 346 IDKHKVNILYTAPTAIRAMMAEGKAAVAGAdgssLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDTWWQTETgACLMTP 425
Cdd:cd05974 172 LVRYGVTTLCAPPTVWRMLIQQDLASFDVK----LREVVGAGEPLNPEVIEQVRRAWGLT---IRDGYGQTET-TALVGN 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 426 LPGaHAMKPGSAAKPFFGVVPALVDNLGNlieGAAEGN--LVILDSWP-GQARTLFGDHDRfvdTYFKTFKGMYFTGDGA 502
Cdd:cd05974 244 SPG-QPVKAGSMGRPLPGYRVALLDPDGA---PATEGEvaLDLGDTRPvGLMKGYAGDPDK---TAHAMRGGYYRTGDIA 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 503 RRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQW 582
Cdd:cd05974 317 MRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETALEIFRF 396
|
490 500 510
....*....|....*....|....*....|....*..
gi 15596084 583 VRREIGPIATPDVIQWAPgLPKTRSGKIMRRILRKIA 619
Cdd:cd05974 397 SRERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
106-611 |
8.71e-52 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 186.26 E-value: 8.71e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05911 9 KELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 ADEGVrggkrtplkANVDDALTNPetSSVQKIIVckrTGAEIKWNQHRDVWYDDLMKVAGST-CAPKEMGAEDPLFILYT 264
Cdd:cd05911 89 DPDGL---------EKVKEAAKEL--GPKDKIIV---LDDKPDGVLSIEDLLSPTLGEEDEDlPPPLKDGKDDTAAILYS 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 265 SGSTGKPKGVLhttggylvyasLTHER-------VFDYRPGEVYWCTADIG-----WVTGHTYIVYGPLaNGATTILFeg 332
Cdd:cd05911 155 SGTTGLPKGVC-----------LSHRNlianlsqVQTFLYGNDGSNDVILGflplyHIYGLFTTLASLL-NGATVIIM-- 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 333 vpNYPDVTRVAKIIDKHKVNILYTAPtAIRAMMAEGKAAVAgADGSSLRLLGSVGEPINPEAwqwyYETVGQ--SRCPIV 410
Cdd:cd05911 221 --PKFDSELFLDLIEKYKITFLYLVP-PIAAALAKSPLLDK-YDLSSLRVILSGGAPLSKEL----QELLAKrfPNATIK 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 411 DTWWQTETGACLMTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDS---WPGqartlfgdhdrfvdt 487
Cdd:cd05911 293 QGYGMTETGGILTVNPDGDD--KPGSVGRLLPNVEAKIVDDDGKDSLGPNEPGEICVRGpqvMKG--------------- 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 488 YFK---------TFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQG 558
Cdd:cd05911 356 YYNnpeatketfDEDGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGEL 435
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 15596084 559 IYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDV-IQWAPGLPKTRSGKIM 611
Cdd:cd05911 436 PRAYVVRKPGEKLTE---KEVKDYVAKKVASYKQLRGgVVFVDEIPKSASGKIL 486
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
102-617 |
3.53e-48 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 176.35 E-value: 3.53e-48
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 102 PADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSK 181
Cdd:cd05926 9 PGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSK 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 182 VVITADEG----VRGGKRTPLkANVD---DALTNPETSSVQKIIVCKRTGAEIKwnqhrdvwyddlmkvagstcAPKEMG 254
Cdd:cd05926 89 LVLTPKGElgpaSRAASKLGL-AILElalDVGVLIRAPSAESLSNLLADKKNAK--------------------SEGVPL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 255 AEDPLFILYTSGSTGKPKGVLHTTGGYLvyASLTH-ERVFDYRPgevywctADIGWVTGHTYIVYGpLANGATTILFEG- 332
Cdd:cd05926 148 PDDLALILHTSGTTGRPKGVPLTHRNLA--ASATNiTNTYKLTP-------DDRTLVVMPLFHVHG-LVASLLSTLAAGg 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 333 ---VPNYPDVTRVAKIIDKHKVNiLYTA-PTAIRAMMAEGKAAVAGADgSSLRLLGSVGEPINPEAWQWYYETVGqsrCP 408
Cdd:cd05926 218 svvLPPRFSASTFWPDVRDYNAT-WYTAvPTIHQILLNRPEPNPESPP-PKLRFIRSCSASLPPAVLEALEATFG---AP 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 409 IVDTWWQTETgACLMT--PLPGAHAmKPGSAAKPFfGVVPALVDNLGNLIEGAAEGNLVILDswPGQARTLFGDHDrfvD 486
Cdd:cd05926 293 VLEAYGMTEA-AHQMTsnPLPPGPR-KPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRG--PNVTRGYLNNPE---A 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 487 TYFKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTL 565
Cdd:cd05926 365 NAEAAFKDGWFrTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVL 444
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 15596084 566 NsgvEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05926 445 R---EGASVTEEELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
82-622 |
4.00e-47 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 175.91 E-value: 4.00e-47
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIW--EGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLActriGAIH 159
Cdd:PRK07529 31 LSRAAARHPDAPALSFllDADPLDRPETWTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWG----GEAA 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 160 SVVF---GGFSPEALAGRIIDCKSKVVITAD-----------EGVRGgKRTPLKANVDDALTNPETSSVQKIIVCKRTGA 225
Cdd:PRK07529 107 GIANpinPLLEPEQIAELLRAAGAKVLVTLGpfpgtdiwqkvAEVLA-ALPELRTVVEVDLARYLPGPKRLAVPLIRRKA 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 226 eikwnQHRDVWYDDLMKV--AGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWC 303
Cdd:PRK07529 186 -----HARILDFDAELARqpGDRLFSGRPIGPDDVAAYFHTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGDTVFC 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 304 TADIGWVTGHTYIVYGPLANGATTIL-----FEGvPNYpdVTRVAKIIDKHKVNILYTAPTAIRAMMaegKAAVAGADGS 378
Cdd:PRK07529 260 GLPLFHVNALLVTGLAPLARGAHVVLatpqgYRG-PGV--IANFWKIVERYRINFLSGVPTVYAALL---QVPVDGHDIS 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 379 SLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQTEtGACLMTPLPGAHAMKPGSAAKPFFG----VVPalVDNLGN 454
Cdd:PRK07529 334 SLRYALCGAAPLPVEVFRRFEAATGV---RIVEGYGLTE-ATCVSSVNPPDGERRIGSVGLRLPYqrvrVVI--LDDAGR 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 455 LIEGAAE---GNLVIldSWPG---------QARTLFGDhDRFVDTyfktfkgmyftGDGARRDEDGYYWITGRVDDVLNV 522
Cdd:PRK07529 408 YLRDCAVdevGVLCI--AGPNvfsgyleaaHNKGLWLE-DGWLNT-----------GDLGRIDADGYFWLTGRAKDLIIR 473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 523 SGHRMGTAEVESAMVAHPKVAEAAVVGmQHDIK-GQGIYVYVTLNSGVEPSEAlrqELKQWVRREIG-PIATPDVIQWAP 600
Cdd:PRK07529 474 GGHNIDPAAIEEALLRHPAVALAAAVG-RPDAHaGELPVAYVQLKPGASATEA---ELLAFARDHIAeRAAVPKHVRILD 549
|
570 580
....*....|....*....|..
gi 15596084 601 GLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK07529 550 ALPKTAVGKIFKPALRRDAIRR 571
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
109-547 |
1.61e-46 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 169.75 E-value: 1.61e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQD-VHRGDVVTIYMPMIPEAVVAMLACTRIGAIHsVVFGGFSPEALAGRII-DCKSKVVITA 186
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAGgVGPGDRVAVLLERSAELVVAILAVLKAGAAY-VPLDPAYPAERLAFILeDAGARLLLTD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEGVRGGKRTPLKANVDDALTNPETSSVqkiivckrtgaeikwnqhrdvwyddlmkVAGSTCAPKEmGAEDPLFILYTSG 266
Cdd:TIGR01733 80 SALASRLAGLVLPVILLDPLELAALDDA----------------------------PAPPPPDAPS-GPDDLAYVIYTSG 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 267 STGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIvYGPLANGATTILFEGVPNYPDVTRVAKII 346
Cdd:TIGR01733 131 STGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDRVLQFASLSFDASVEEI-FGALLAGATLVVPPEDEERDDAALLAALI 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 347 DKHKVNILYTAPTAIRAMMAEGKAAvagadGSSLRLLGSVGEPINPEAWQWYYETVGQSRcpIVDTWWQTE-TGACLMTP 425
Cdd:TIGR01733 209 AEHPVTVLNLTPSLLALLAAALPPA-----LASLRLVILGGEALTPALVDRWRARGPGAR--LINLYGPTEtTVWSTATL 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 426 LPGAHAMKPGSAA--KPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHD----RFVDTYFKTFKG--MYF 497
Cdd:TIGR01733 282 VDPDDAPRESPVPigRPLANTRLYVLDDDLRPVPVGVVGELYI--GGPGVARGYLNRPEltaeRFVPDPFAGGDGarLYR 359
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|
gi 15596084 498 TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAV 547
Cdd:TIGR01733 360 TGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
82-617 |
2.62e-46 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 171.63 E-value: 2.62e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:PRK07656 11 LARAARRFGDKEAYVFGD------QRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVP 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADEGVrggkrtPLKANVDDALTNpetssVQKIIVCKRTGAEIKWNQHRDvwYDDLM 241
Cdd:PRK07656 85 LNTRYTADEAAYILARGDAKALFVLGLFL------GVDYSATTRLPA-----LEHVVICETEEDDPHTEKMKT--FTDFL 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 KVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLhttggylvyasLTHERVF----------DYRPGEVYWCTADIGWVT 311
Cdd:PRK07656 152 AAGDPAERAPEVDPDDVADILFTSGTTGRPKGAM-----------LTHRQLLsnaadwaeylGLTEGDRYLAANPFFHVF 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 312 GHTYIVYGPLANGATTILfegVPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMM-AEGKAAvagADGSSLRLLGSVGEPI 390
Cdd:PRK07656 221 GYKAGVNAPLMRGATILP---LPVF-DPDEVFRLIETERITVLPGPPTMYNSLLqHPDRSA---EDLSSLRLAVTGAASM 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 391 NPEAWQWYYETVGqsrCPIVDTWWQ-TE-TGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVI-- 466
Cdd:PRK07656 294 PVALLERFESELG---VDIVLTGYGlSEaSGVTTFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLVrg 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 467 -------LDSWPGQARTLFGDhdrfvdtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAH 539
Cdd:PRK07656 371 pnvmkgyYDDPEATAAAIDAD-------------GWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEH 437
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 540 PKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK07656 438 PAVAEAAVIGVPDERLGEVGKAYVVLKPGAELTE---EELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
91-623 |
9.10e-46 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 170.11 E-value: 9.10e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEgddpadHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEA 170
Cdd:PRK08316 26 DKTALVFG------DRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 171 LAGRIIDCKSKVVITADEgvrggkrtpLKANVDDALTnpETSSVQKIIVCKRTGAEikwnqHRDVWYD-DLMKVAGSTCA 249
Cdd:PRK08316 100 LAYILDHSGARAFLVDPA---------LAPTAEAALA--LLPVDTLILSLVLGGRE-----APGGWLDfADWAEAGSVAE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 250 PK-EMGAEDPLFILYTSGSTGKPKGVLHTTGgylvyaSLTHERV-----FDYRPGEV-------YWCTAdigwvtGHTYI 316
Cdd:PRK08316 164 PDvELADDDLAQILYTSGTESLPKGAMLTHR------ALIAEYVscivaGDMSADDIplhalplYHCAQ------LDVFL 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 vyGP-LANGATTILFEGvpnyPDVTRVAKIIDKHKVNILYTAPTAIRAMMaeGKAAVAGADGSSLRlLGSVGEPINPeaw 395
Cdd:PRK08316 232 --GPyLYVGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLL--RHPDFDTRDLSSLR-KGYYGASIMP--- 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 396 qwyyETV---GQSRCPIVDTW---WQTETGAcLMTPL-PGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVild 468
Cdd:PRK08316 300 ----VEVlkeLRERLPGLRFYncyGQTEIAP-LATVLgPEEHLRRPGSAGRPVLNVETRVVDDDGNDVAPGEVGEIV--- 371
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 469 swpgqART---LFGdhdrfvdtYF----KT---FKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMV 537
Cdd:PRK08316 372 -----HRSpqlMLG--------YWddpeKTaeaFRGGWFhSGDLGVMDEEGYITVVDRKKDMIKTGGENVASREVEEALY 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 538 AHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK08316 439 THPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKVPKRVIFVDELPRNPSGKILKRELRE 515
|
....*.
gi 15596084 618 IAAAEY 623
Cdd:PRK08316 516 RYAGAF 521
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
84-616 |
1.30e-45 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 169.06 E-value: 1.30e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVF 163
Cdd:cd17651 3 RQAARTPDAPALVAEG------RRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 164 GGFSPEALAGRIIDCKSKVVITADEgvrggkrtplkanVDDALTNPEtssvqkiivckrtgaeikwnqhRDVWYDDLMKV 243
Cdd:cd17651 77 PAYPAERLAFMLADAGPVLVLTHPA-------------LAGELAVEL----------------------VAVTLLDQPGA 121
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 244 AGSTCAPK--EMGAEDPLFILYTSGSTGKPKGVL--HTTGGYLVYAsltHERVFDYRPGEVYWCTADIGWVTGhTYIVYG 319
Cdd:cd17651 122 AAGADAEPdpALDADDLAYVIYTSGSTGRPKGVVmpHRSLANLVAW---QARASSLGPGARTLQFAGLGFDVS-VQEIFS 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 320 PLANGATTILfegVPNY--PDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLGSVGEPINPEAWQW 397
Cdd:cd17651 198 TLCAGATLVL---PPEEvrTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPL--GVRLAALRYLLTGGEQLVLTEDLR 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 398 YYeTVGQSRCPIVDTWWQTET--GACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQAR 475
Cdd:cd17651 273 EF-CAGLPGLRLHNHYGPTEThvVTALSLPGDPAAWPAPPPIGRPIDNTRVYVLDAALRPVPPGVPGELYI--GGAGLAR 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 476 TLFGD----HDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQ 551
Cdd:cd17651 350 GYLNRpeltAERFVPDPFVPGARMYRTGDLARWLPDGELEFLGRADDQVKIRGFRIELGEIEAALARHPGVREAVVLARE 429
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 552 HDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd17651 430 DRPGEKRLVAYVVGDPEAPVDAA---ELRAALATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
109-617 |
3.78e-45 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 166.40 E-value: 3.78e-45
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:cd05903 3 TYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVPER 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 189 gvrggkrtplkanvddaltnpetssvqkiivckrtgaeikWNQHRdvwyddlmkvagstcaPKEMGAeDPLFILYTSGST 268
Cdd:cd05903 83 ----------------------------------------FRQFD----------------PAAMPD-AVALLLFTSGTT 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 269 GKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVpnypDVTRVAKIIDK 348
Cdd:cd05903 106 GEPKGVMHSHNT-LSASIRQYAERLGLGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIW----DPDKALALMRE 180
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 349 HKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPIvdtWWQTETGACLMTPLPG 428
Cdd:cd05903 181 HGVTFMMGATPFLTDLLNAVEEA--GEPLSRLRTFVCGGATVPRSLARRAAELLGAKVCSA---YGSTECPGAVTSITPA 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 429 AHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDswpgqARTLFGDHDRFVDTYFKTFKGMYFTGDGARRDEDG 508
Cdd:cd05903 256 PEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG-----PSVFLGYLDRPDLTADAAPEGWFRTGDLARLDEDG 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 509 YYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPS-EALRQELKqwvRREI 587
Cdd:cd05903 331 YLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGALLTfDELVAYLD---RQGV 407
|
490 500 510
....*....|....*....|....*....|
gi 15596084 588 GPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05903 408 AKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
139-616 |
1.23e-44 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 166.53 E-value: 1.23e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 139 MPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVRGGKRTPLKANVDDAltnpetsSVQKII 218
Cdd:PLN03051 1 MPMTVDAVIIYLAIVLAGCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKVVEA-------APAKAI 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 219 VCKRTGAEIKWNQHRDV--WYDDLMKVAGSTCA------PKEMGAEDPLFILYTSGSTGKPKGV--LHTTGGYLVYASLT 288
Cdd:PLN03051 74 VLPAAGEPVAVPLREQDlsWCDFLGVAAAQGSVggneysPVYAPVESVTNILFSSGTTGEPKAIpwTHLSPLRCASDGWA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 289 HervFDYRPGEVYWCTADIGWVTGhTYIVYGPLANGATTILFEGVPNYPDvtrVAKIIDKHKVNILYTAPTAIRAMMAEG 368
Cdd:PLN03051 154 H---MDIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRG---FGKFVQDAGVTVLGLVPSIVKAWRHTG 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 369 KAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRcPIVDTWWQTE--TGACLMTPLpgaHAMKPGSAAKPFFGVVP 446
Cdd:PLN03051 227 AFAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTElaSGYISSTLL---QPQAPGAFSTASLGTRF 302
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 447 ALVDNLGNLI--EGAAEGNLVILDSWPGQA-RTLFGDHDRfvdTYFK-----TFKGMYFT--GDGARRDEDGYYWITGRV 516
Cdd:PLN03051 303 VLLNDNGVPYpdDQPCVGEVALAPPMLGASdRLLNADHDK---VYYKgmpmyGSKGMPLRrhGDIMKRTPGGYFCVQGRA 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 517 DDVLNVSGHRMGTAEVESAMV-AHPKVAEAAVVGMQhDIKGQGIYVYVTLNSGVEPS-------EALRQELKQWVRREIG 588
Cdd:PLN03051 380 DDTMNLGGIKTSSVEIERACDrAVAGIAETAAVGVA-PPDGGPELLVIFLVLGEEKKgfdqarpEALQKKFQEAIQTNLN 458
|
490 500
....*....|....*....|....*...
gi 15596084 589 PIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PLN03051 459 PLFKVSRVKIVPELPRNASNKLLRRVLR 486
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
106-616 |
2.08e-44 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 164.00 E-value: 2.08e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05934 2 RRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 adegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgaeDPLFILYTS 265
Cdd:cd05934 82 -----------------------------------------------------------------------DPASILYTS 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLhTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILfegVPNYpDVTRVAKI 345
Cdd:cd05934 91 GTTGPPKGVV-ITHANLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVL---LPRF-SASRFWSD 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 346 IDKHKVNILYTAPTAIRAMMAEGKAAvagaDGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETGACLMTP 425
Cdd:cd05934 166 VRRYGATVTNYLGAMLSYLLAQPPSP----DDRAHRLRAAYGAPNPPELHEEFEERFG---VRLLEGYGMTETIVGVIGP 238
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 426 LPgaHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIlDSWPGQARTLfGDHDRFVDTYfKTFKGMYF-TGDGARR 504
Cdd:cd05934 239 RD--EPRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVI-RGLRGWGFFK-GYYNMPEATA-EAMRNGWFhTGDLGYR 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 505 DEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVR 584
Cdd:cd05934 314 DADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE---ELFAFCE 390
|
490 500 510
....*....|....*....|....*....|..
gi 15596084 585 REIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05934 391 GQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| ligase_PEP_1 |
TIGR03098 |
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an ... |
79-617 |
3.27e-43 |
|
acyl-CoA ligase (AMP-forming), exosortase A-associated; This group of proteins contains an AMP-binding domain (pfam00501) associated with acyl CoA-ligases. These proteins are generally found in genomes containing the exosortase/PEP-CTERM protein expoert system, specifically the type 1 variant of this system described by the Genome Property GenProp0652. When found in this context they are invariably present next to a decarboxylase enzyme. A number of sequences from Burkholderia species also hit this model, but the genomic context is obviously different. The hypothesis of a constant substrate for this family is only strong where the exosortase context is present.
Pssm-ID: 211788 [Multi-domain] Cd Length: 517 Bit Score: 163.03 E-value: 3.27e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:TIGR03098 3 HHLLEDAAARLPDATALVHHD------RTLTYAALSERVLALASGLRGLGLARGERVAIYLDKRLETVTAMFGAALAGGV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITADEGVRGGKRTPlkANVDDALTNPETSSVQKIIVCKRTGAEIKWNQhrdvwyd 238
Cdd:TIGR03098 77 FVPINPLLKAEQVAHILADCNVRLLVTSSERLDLLHPAL--PGCHDLRTLIIVGDPAHASEGHPGEEPASWPK------- 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 dlMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVL--HTTggyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYI 316
Cdd:TIGR03098 148 --LLALGDADPPHPVIDSDMAAILYTSGSTGRPKGVVlsHRN---LVAGAQSVATYLENRPDDRLLAVLPLSFDYGFNQL 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 VYGpLANGATTILFegvpNYPDVTRVAKIIDKHKVNILYTAPtAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINPEAWQ 396
Cdd:TIGR03098 223 TTA-FYVGATVVLH----DYLLPRDVLKALEKHGITGLAAVP-PLWAQLAQ--LDWPESAAPSLRYLTNSGGAMPRATLS 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 397 WYYETVGQSRcpIVDTWWQTEtgACLMTPLPGAHA-MKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLV------ILDS 469
Cdd:TIGR03098 295 RLRSFLPNAR--LFLMYGLTE--AFRSTYLPPEEVdRRPDSIGKAIPNAEVLVLREDGSECAPGEEGELVhrgalvAMGY 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 470 W--PGQARTLFGDHDRFVDTyfKTFKGM-YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAA 546
Cdd:TIGR03098 371 WndPEKTAERFRPLPPFPGE--LHLPELaVWSGDTVRRDEEGFLYFVGRRDEMIKTSGYRVSPTEVEEVAYATGLVAEAV 448
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15596084 547 VVGMQHDIKGQGIYVYVTLNSGVepsEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:TIGR03098 449 AFGVPDPTLGQAIVLVVTPPGGE---ELDRAALLAECRARLPNYMVPALIHVRQALPRNANGKIDRKALAK 516
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
82-622 |
1.25e-42 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 161.76 E-value: 1.25e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:PRK13295 30 LDACVASCPDKTAVTAVRLGTGAPRRFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNP 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADEgVRGGKRTPLKANVddaltNPETSSVQKIIVCKRTGA--------EIKWNQHR 233
Cdd:PRK13295 110 LMPIFRERELSFMLKHAESKVLVVPKT-FRGFDHAAMARRL-----RPELPALRHVVVVGGDGAdsfealliTPAWEQEP 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 234 DvwyddlmkvAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTT----GGYLVYAslthERVfDYRPGEVYWCTADIGW 309
Cdd:PRK13295 184 D---------APAILARLRPGPDDVTQLIYTSGTTGEPKGVMHTAntlmANIVPYA----ERL-GLGADDVILMASPMAH 249
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 310 VTGHTYIVYGPLANGATTILFEgvpnYPDVTRVAKIIDKHKVNilYT-APTAIRAMMAEGKAAvAGADGSSLRLLGSVGE 388
Cdd:PRK13295 250 QTGFMYGLMMPVMLGATAVLQD----IWDPARAAELIRTEGVT--FTmASTPFLTDLTRAVKE-SGRPVSSLRTFLCAGA 322
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 389 PINPEAWQWYYETVGQSrcpIVDTWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIld 468
Cdd:PRK13295 323 PIPGALVERARAALGAK---IVSAWGMTENGAVTLTKLDDPDERASTTDGCPLPGVEVRVVDADGAPLPAGQIGRLQV-- 397
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 469 swpgQARTLFGDHDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVV 548
Cdd:PRK13295 398 ----RGCSNFGGYLKRPQLNGTDADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIV 473
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 549 GMQHDIKGQGIYVYVTLnsgvEPSEALRQE-LKQWVRREigPIAT---PDVIQWAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK13295 474 AYPDERLGERACAFVVP----RPGQSLDFEeMVEFLKAQ--KVAKqyiPERLVVRDALPRTPSGKIQKFRLREMLRGE 545
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
80-617 |
2.28e-42 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 161.10 E-value: 2.28e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 80 NCLDRHLAERGDQVAIIWEGDDpadhqeITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIH 159
Cdd:PRK07786 21 NQLARHALMQPDAPALRFLGNT------TTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIA 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 160 SVVFGGFSPEALAGRIIDCKSKVVITadEGVRggkrTPLKANVDDALTNPETSsvqkIIVCKRTGAEIkwnqhrdVWYDD 239
Cdd:PRK07786 95 VPVNFRLTPPEIAFLVSDCGAHVVVT--EAAL----APVATAVRDIVPLLSTV----VVAGGSSDDSV-------LGYED 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 240 LMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKG-VL-HTTggyLVYASLTHERVFDY-RPGEVYWCTADIGWVTGHTYI 316
Cdd:PRK07786 158 LLAEAGPAHAPVDIPNDSPALIMYTSGTTGRPKGaVLtHAN---LTGQAMTCLRTNGAdINSDVGFVGVPLFHIAGIGSM 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 VYGpLANGATTILfegvpnYP----DVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADgSSLRLLGSVGEPINP 392
Cdd:PRK07786 235 LPG-LLLGAPTVI------YPlgafDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQAR--PRD-LALRVLSWGAAPASD 304
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 393 EAWQWYYETVGQSRcpIVDTWWQTEtgaclMTP----LPGAHAM-KPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIl 467
Cdd:PRK07786 305 TLLRQMAATFPEAQ--ILAAFGQTE-----MSPvtcmLLGEDAIrKLGSVGKVIPTVAARVVDENMNDVPVGEVGEIVY- 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 dswpgQARTLFGDHDRFVDTYFKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAA 546
Cdd:PRK07786 377 -----RAPTLMSGYWNNPEATAEAFAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVA 451
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15596084 547 VVGMQHDIKGQGIYVYVTLNSGVEPSEAlrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK07786 452 VIGRADEKWGEVPVAVAAVRNDDAALTL--EDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
91-615 |
1.62e-41 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 156.70 E-value: 1.62e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWegddpaDHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAihsvVFGGFSPEA 170
Cdd:cd17643 2 EAVAVVD------EDRRLTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGG----AYVPIDPAY 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 171 LAGRIIdckskvVITADEGVRGgkrtplkanvddALTNPEtssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcap 250
Cdd:cd17643 72 PVERIA------FILADSGPSL------------LLTDPD---------------------------------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 251 kemgaeDPLFILYTSGSTGKPKGVLHTTGGylVYASLTH-ERVFDYRPGEVywctadigWVTGHTYI-------VYGPLA 322
Cdd:cd17643 94 ------DLAYVIYTSGSTGRPKGVVVSHAN--VLALFAAtQRWFGFNEDDV--------WTLFHSYAfdfsvweIWGALL 157
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 323 NGATTILfegVPNypDVTR----VAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINPEAWQWY 398
Cdd:cd17643 158 HGGRLVV---VPY--EVARspedFARLLRDEGVTVLNQTPSAFYQLVEA--ADRDGRDPLALRYVIFGGEALEAAMLRPW 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 399 YETVGQSRCPIVDTWWQTETgACLMTPLPGAHAMKPGSAAKPFFGVVP----ALVDNLGNLIEGAAEGNLVIldSWPGQA 474
Cdd:cd17643 231 AGRFGLDRPQLVNMYGITET-TVHVTFRPLDAADLPAAAASPIGRPLPglrvYVLDADGRPVPPGVVGELYV--SGAGVA 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFG----DHDRFV-DTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd17643 308 RGYLGrpelTAERFVaNPFGGPGSRMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAALATHPSVRDAAVIV 387
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15596084 550 MQHDIKGQGIYVYVTLNSGvepSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17643 388 REDEPGDTRLVAYVVADDG---AAADIAELRALLKELLPDYMVPARYVPLDALPLTVNGKLDRAAL 450
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
79-615 |
1.27e-40 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 155.13 E-value: 1.27e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:cd17646 1 HALVAEQAARTPDAPAVVDEG------RTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVIT-ADEGVRGGKRTPLKANVDDALTNPETSsvqkiivckRTGAEIkwnqhrdvwy 237
Cdd:cd17646 75 YLPLDPGYPADRLAYMLADAGPAVVLTtADLAARLPAGGDVALLGDEALAAPPAT---------PPLVPP---------- 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 238 ddlmkvagstcapkemGAEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGW-VTGhtYI 316
Cdd:cd17646 136 ----------------RPDNLAYVIYTSGSTGRPKGVMVTHAG-IVNRLLWMQDEYPLGPGDRVLQKTPLSFdVSV--WE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 VYGPLANGATTILFEgVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkaaVAGADGSSLRLLGSVGEPINPEAWQ 396
Cdd:cd17646 197 LFWPLVAGARLVVAR-PGGHRDPAYLAALIREHGVTTCHFVPSMLRVFLAE----PAAGSCASLRRVFCSGEALPPELAA 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 397 WYYETVGqsrCPIVDTWWQTETgACLMT--PLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQA 474
Cdd:cd17646 272 RFLALPG---AELHNLYGPTEA-AIDVThwPVRGPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELYL--GGVQLA 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFG----DHDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGM 550
Cdd:cd17646 346 RGYLGrpalTAERFVPDPFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAVTHAVVVAR 425
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 551 QHDIKGQGIYVYVTLNSGVEPseALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17646 426 AAPAGAARLVGYVVPAAGAAG--PDTAALRAHLAERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
87-622 |
1.59e-40 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 155.89 E-value: 1.59e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 87 AER-GDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFG 164
Cdd:PRK08314 20 ARRyPDKTAIVFYG------RAISYRELLEEAERLAGYLQQEcGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 165 GFSPEALAGRIIDCKSKVVIT----ADEGVRGGKRTPLK----ANVDDALTN------PETSSVQKIIVCKRTGAEIKWN 230
Cdd:PRK08314 94 MNREEELAHYVTDSGARVAIVgselAPKVAPAVGNLRLRhvivAQYSDYLPAepeiavPAWLRAEPPLQALAPGGVVAWK 173
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 231 QHRDvwyddlmkvAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGgylvyaSLTHERV-----FDYRPGEVYWCTA 305
Cdd:PRK08314 174 EALA---------AGLAPPPHTAGPDDLAVLPYTSGTTGVPKGCMHTHR------TVMANAVgsvlwSNSTPESVVLAVL 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 306 DIGWVTGHTYIVYGPLANGATTILfegVPNYpDVTRVAKIIDKHKVnilyTAPTAIRAMMAE--GKAAVAGADGSSLRLL 383
Cdd:PRK08314 239 PLFHVTGMVHSMNAPIYAGATVVL---MPRW-DREAAARLIERYRV----THWTNIPTMVVDflASPGLAERDLSSLRYI 310
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPInPEA-WQWYYETVGqsrCPIVDTWWQTETGA-CLMTPlpgAHAMKPGSAAKPFFGVVPALVD--NLGNLIEGa 459
Cdd:PRK08314 311 GGGGAAM-PEAvAERLKELTG---LDYVEGYGLTETMAqTHSNP---PDRPKLQCLGIPTFGVDARVIDpeTLEELPPG- 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 460 AEGNLVI------LDSWPGQARTlfgdhdrfvDTYFKTFKGMYF--TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAE 531
Cdd:PRK08314 383 EVGEIVVhgpqvfKGYWNRPEAT---------AEAFIEIDGKRFfrTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAE 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 532 VESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGvEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIM 611
Cdd:PRK08314 454 VENLLYKHPAIQEACVIATPDPRRGETVKAVVVLRPE-ARGKTTEEEIIAWAREHMAAYKYPRIVEFVDSLPKSGSGKIL 532
|
570
....*....|.
gi 15596084 612 RRILRKIAAAE 622
Cdd:PRK08314 533 WRQLQEQEKAR 543
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
83-615 |
1.84e-40 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 154.28 E-value: 1.84e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 83 DRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVV 162
Cdd:cd12117 4 EEQAARTPDAVAVVYGD------RSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 163 FGGFSPEALAGRIIDCKSKVVIT--ADEGVRGGKRTPLKANVDDaltnpetssvqkiivckRTGAEikwnqhrdvwyddl 240
Cdd:cd12117 78 DPELPAERLAFMLADAGAKVLLTdrSLAGRAGGLEVAVVIDEAL-----------------DAGPA-------------- 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 241 mKVAGSTCAPkemgaEDPLFILYTSGSTGKPKGVLHTTGGYLvyaSLTHERVF-DYRPGEVYWCTADIGWvTGHTYIVYG 319
Cdd:cd12117 127 -GNPAVPVSP-----DDLAYVMYTSGSTGRPKGVAVTHRGVV---RLVKNTNYvTLGPDDRVLQTSPLAF-DASTFEIWG 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 320 PLANGATTILFE-GVPnyPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGadgssLRLLGSVGEPINPEAWQWY 398
Cdd:cd12117 197 ALLNGARLVLAPkGTL--LDPDALGALIAEEGVTVLWLTAALFNQLADEDPECFAG-----LRELLTGGEVVSPPHVRRV 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 399 YEtvgqsRCP---IVDTWWQTETgaclmTPLPGAHAMKPGSAA-------KPFFGVVPALVDNLGNLIEGAAEGNLVIld 468
Cdd:cd12117 270 LA-----ACPglrLVNGYGPTEN-----TTFTTSHVVTELDEVagsipigRPIANTRVYVLDEDGRPVPPGVPGELYV-- 337
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 469 SWPGQARTLFGDHD----RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAE 544
Cdd:cd12117 338 GGDGLALGYLNRPAltaeRFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVRE 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15596084 545 AAVVGMQHDIKGQGIYVYVTLNSGVEPsealrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12117 418 AVVVVREDAGGDKRLVAYVVAEGALDA-----AELRAFLRERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
87-615 |
5.25e-40 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 153.54 E-value: 5.25e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 87 AERGDQVAIIwegdDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGF 166
Cdd:cd05904 16 SAHPSRPALI----DAATGRALTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLS 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 167 SPEALAGRIIDCKSKVVITADEGVrggKRTPlkanvddaltnpetSSVQKIIVCKRTgaeikwnqHRDVWY-DDLMKVAG 245
Cdd:cd05904 92 TPAEIAKQVKDSGAKLAFTTAELA---EKLA--------------SLALPVVLLDSA--------EFDSLSfSDLLFEAD 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 246 STCAPK-EMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERV-FDYRPGEVYWCTADIGWVTGHTYIVYGPLAN 323
Cdd:cd05904 147 EAEPPVvVIKQDDVAALLYSSGTTGRSKGVMLTHRNLIAMVAQFVAGEgSNSDSEDVFLCVLPMFHIYGLSSFALGLLRL 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 324 GATTILfegVPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAegKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVG 403
Cdd:cd05904 227 GATVVV---MPRF-DLEELLAAIERYKVTHLPVVPPIVLALVK--SPIVDKYDLSSLRQIMSGAAPLGKELIEAFRAKFP 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 404 QsrCPIVDTWWQTETGACL-MTPLPGAHAMKPGSAakpffgvvpalvdnlGNLIEGaAEGNLVILDSW----PGQARTLF 478
Cdd:cd05904 301 N--VDLGQGYGMTESTGVVaMCFAPEKDRAKYGSV---------------GRLVPN-VEAKIVDPETGeslpPNQTGELW 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 479 GDHDRFVDTYFK---------TFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd05904 363 IRGPSIMKGYLNnpeataatiDKEGWLHTGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIP 442
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 550 MQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGP------IATPDVIqwapglPKTRSGKIMRRIL 615
Cdd:cd05904 443 YPDEEAGEVPMAFVVRKPGSSLTE---DEIMDFVAKQVAPykkvrkVAFVDAI------PKSPSGKILRKEL 505
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
86-615 |
3.27e-39 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 150.09 E-value: 3.27e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 86 LAERGDQVAIIWEGDDpadhqeITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGG 165
Cdd:cd05945 1 AAANPDRPAVVEGGRT------LTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDAS 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 166 FSPEALAGRIIDCKSKVVITADEgvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvag 245
Cdd:cd05945 75 SPAERIREILDAAKPALLIADGD--------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 246 stcapkemgaeDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERvFDYRPGEVYWCTA----DIGwvtghTYIVYGPL 321
Cdd:cd05945 98 -----------DNAYIIFTSGSTGRPKGVQISHDNLVSFTNWMLSD-FPLGPGDVFLNQApfsfDLS-----VMDLYPAL 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 ANGATTILfegVPN--YPDVTRVAKIIDKHKVNILYTAPTAIRamMAEGKAAVAGADGSSLRLLGSVGEPI-NPEAWQWy 398
Cdd:cd05945 161 ASGATLVP---VPRdaTADPKQLFRFLAEHGITVWVSTPSFAA--MCLLSPTFTPESLPSLRHFLFCGEVLpHKTARAL- 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 399 yetvgQSR---CPIVDTWWQTE-TGACL-----------MTPLPGAHAmKPGSAAKpffgvvpaLVDNLGNLIEGAAEGN 463
Cdd:cd05945 235 -----QQRfpdARIYNTYGPTEaTVAVTyievtpevldgYDRLPIGYA-KPGAKLV--------ILDEDGRPVPPGEKGE 300
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 464 LVIldSWPGQARTLFGD---HDRFvdtyFKTFKGM--YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVA 538
Cdd:cd05945 301 LVI--SGPSVSKGYLNNpekTAAA----FFPDEGQraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQ 374
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 539 HPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPseALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05945 375 VPGVKEAVVVPKYKGEKVTELIAFVVPKPGAEA--GLTKAIKAELAERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
82-621 |
5.64e-39 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 151.07 E-value: 5.64e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsV 161
Cdd:COG1021 31 LRRRAERHPDRIAVV------DGERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAI--P 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSP-----EALAGRIidcKSKVVITADegvrggkRTPLKANVD--DALTNpETSSVQKIIVCKRTGAEIKWnqhrd 234
Cdd:COG1021 103 VFALPAHrraeiSHFAEQS---EAVAYIIPD-------RHRGFDYRAlaRELQA-EVPSLRHVLVVGDAGEFTSL----- 166
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 235 vwyDDLMKVAGSTCAPkEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLvYASLTHERVFDYRPGEVYWCTADIGwvtgHT 314
Cdd:COG1021 167 ---DALLAAPADLSEP-RPDPDDVAFFQLSGGTTGLPKLIPRTHDDYL-YSVRASAEICGLDADTVYLAALPAA----HN 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 315 YI-----VYGPLANGATTILfegVPNyPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEP 389
Cdd:COG1021 238 FPlsspgVLGVLYAGGTVVL---APD-PSPDTAFPLIERERVTVTALVPPLALLWLDA--AERSRYDLSSLRVLQVGGAK 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 390 INPEA------------WQWY-------------------YETVGQSRCP-----IVDtwwqtETGaclmTPLPgahamk 433
Cdd:COG1021 312 LSPELarrvrpalgctlQQVFgmaeglvnytrlddpeeviLTTQGRPISPddevrIVD-----EDG----NPVP------ 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 434 PGSAakpffgvvpalvdnlGNL-------IEG---AAEGNlvildswpgqaRTLFgDHDrfvdtyfktfkGMYFTGDGAR 503
Cdd:COG1021 377 PGEV---------------GELltrgpytIRGyyrAPEHN-----------ARAF-TPD-----------GFYRTGDLVR 418
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNsGVEPSealRQELKQWV 583
Cdd:COG1021 419 RTPDGYLVVEGRAKDQINRGGEKIAAEEVENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPR-GEPLT---LAELRRFL 494
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 15596084 584 rREIGpIAT---PDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:COG1021 495 -RERG-LAAfklPDRLEFVDALPLTAVGKIDKKALRAALAA 533
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
91-616 |
1.07e-37 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 145.97 E-value: 1.07e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEA 170
Cdd:cd17649 2 DAVALVFGD------QSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGAYVPLDPEYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 171 LAGRIIDCKSKVVITADegvrggkrtplkanvddaltnPETssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcap 250
Cdd:cd17649 76 LRYMLEDSGAGLLLTHH---------------------PRQ--------------------------------------- 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 251 kemgaedPLFILYTSGSTGKPKGVLhttggyLVYASLTHE-----RVFDYRPGEVYWCTADIGWVTGHTYiVYGPLANGA 325
Cdd:cd17649 96 -------LAYVIYTSGSTGTPKGVA------VSHGPLAAHcqataERYGLTPGDRELQFASFNFDGAHEQ-LLPPLICGA 161
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 326 TTILfEGVPNYPDVTRVAKIIDKHKVNILyTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPE-AWQWyyetvGQ 404
Cdd:cd17649 162 CVVL-RPDELWASADELAEMVRELGVTVL-DLPPAYLQQLAEEADRTGDGRPPSLRLYIFGGEALSPElLRRW-----LK 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 405 SRCPIVDTWWQTETgacLMTPL--PGAHAMKPGSAAKPFFGVVPA----LVDNLGNLIEGAAEGNLVIldSWPGQARTLF 478
Cdd:cd17649 235 APVRLFNAYGPTEA---TVTPLvwKCEAGAARAGASMPIGRPLGGrsayILDADLNPVPVGVTGELYI--GGEGLARGYL 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 479 G----DHDRFV-DTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGmQHD 553
Cdd:cd17649 310 GrpelTAERFVpDPFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVA-LDG 388
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15596084 554 IKGQGIYVYVTLNSGvEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd17649 389 AGGKQLVAYVVLRAA-AAQPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
91-617 |
2.11e-37 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 144.74 E-value: 2.11e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPE 169
Cdd:cd05941 1 DRIAIV------DDGDSITYADLVARAARLANRLLALgKDLRGDRVAFLAPPSAEYVVAQLAIWRAGGVAVPLNPSYPLA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 170 ALAGRIIDCKSKVVItadegvrggkrtplkanvDDALtnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstca 249
Cdd:cd05941 75 ELEYVITDSEPSLVL------------------DPAL------------------------------------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 250 pkemgaedplfILYTSGSTGKPKGVLHTTGGYL-VYASLTHErvFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTI 328
Cdd:cd05941 94 -----------ILYTSGTTGRPKGVVLTHANLAaNVRALVDA--WRWTEDDVLLHVLPLHHVHGLVNALLCPLFAGASVE 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 329 LfegVPNYpDVTRVAKIIDKHKVNILYTAPT-------AIRAMMAEGKAAVAGADgSSLRLLGSVGEPINPEAWQWYYET 401
Cdd:cd05941 161 F---LPKF-DPKEVAISRLMPSITVFMGVPTiytrllqYYEAHFTDPQFARAAAA-ERLRLMVSGSAALPVPTLEEWEAI 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 402 VGQsrcPIVDTWWQTETGACLMTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAE-------GNLVILDSWPGQA 474
Cdd:cd05941 236 TGH---TLLERYGMTEIGMALSNPLDGER--RPGTVGMPLPGVQARIVDEETGEPLPRGEvgeiqvrGPSVFKEYWNKPE 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTlfgdHDRFV-DTYFKTfkgmyftGDGARRDEDGYYWITGRV-DDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQH 552
Cdd:cd05941 311 AT----KEEFTdDGWFKT-------GDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPD 379
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 553 DIKGQGIYVYVTLNSGVEPSEAlrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05941 380 PDWGERVVAVVVLRAGAAALSL--EELKEWAKQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
82-622 |
3.42e-37 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 149.24 E-value: 3.42e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGA---- 157
Cdd:COG1020 482 FEAQAARTPDAVAVVFGD------QSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAayvp 555
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 158 IHSvvfgGFSPEALAGRIIDCKSKVVITAdegvrggkrtplkANVDDALTNpetSSVQKIIVckrtgaeikwnqhrdvwy 237
Cdd:COG1020 556 LDP----AYPAERLAYMLEDAGARLVLTQ-------------SALAARLPE---LGVPVLAL------------------ 597
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 238 DDLMKVAGSTCAPK-EMGAEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVyW-----CTADIGwvt 311
Cdd:COG1020 598 DALALAAEPATNPPvPVTPDDLAYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDR-VlqfasLSFDAS--- 672
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 312 ghTYIVYGPLANGATTILF-EGVPnyPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVagadgSSLRLLGSVGEPI 390
Cdd:COG1020 673 --VWEIFGALLSGATLVLApPEAR--RDPAALAELLARHRVTVLNLTPSLLRALLDAAPEAL-----PSLRLVLVGGEAL 743
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 391 NPEAWQWYYETVGQsrCPIVDTWWQTET--GACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLI-EGAAeGNLVIl 467
Cdd:COG1020 744 PPELVRRWRARLPG--ARLVNLYGPTETtvDSTYYEVTPPDADGGSVPIGRPIANTRVYVLDAHLQPVpVGVP-GELYI- 819
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 dSWPGQARTLFGDHD----RFVDTYFkTFKG--MYFTGDGARRDEDG---YywiTGRVDDVLNVSGHRMGTAEVESAMVA 538
Cdd:COG1020 820 -GGAGLARGYLNRPEltaeRFVADPF-GFPGarLYRTGDLARWLPDGnleF---LGRADDQVKIRGFRIELGEIEAALLQ 894
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 539 HPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRReigPIATPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:COG1020 895 HPGVREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLALALLLP---PYMVPAAVVLLLPLPLTGNGKLDRLALPAP 971
|
....
gi 15596084 619 AAAE 622
Cdd:COG1020 972 AAAA 975
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
78-615 |
1.45e-36 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 142.46 E-value: 1.45e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 78 SHNCLDRHLAERGDQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGA 157
Cdd:cd12115 1 LHDLVEAQAARTPDAIALV------CGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 158 IHSVVFGGFSPEALAGRIIDCKSKVVITadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwy 237
Cdd:cd12115 75 AYVPLDPAYPPERLRFILEDAQARLVLT---------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 238 ddlmkvagstcapkemGAEDPLFILYTSGSTGKPKGVL--HTTGGYLVYASLTHervfdyrpgevywCTAD-IGWVTGHT 314
Cdd:cd12115 103 ----------------DPDDLAYVIYTSGSTGRPKGVAieHRNAAAFLQWAAAA-------------FSAEeLAGVLAST 153
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 315 YI--------VYGPLANGATTILFEGVPNYPDVTRVAKiidkhkVNILYTAPTAIRAMMAegkaavAGADGSSLRLLGSV 386
Cdd:cd12115 154 SIcfdlsvfeLFGPLATGGKVVLADNVLALPDLPAAAE------VTLINTVPSAAAELLR------HDALPASVRVVNLA 221
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 387 GEPINPEAWQWYYETVGQSRC-----PIVDTWWQTetgACLMTPlpgaHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAE 461
Cdd:cd12115 222 GEPLPRDLVQRLYARLQVERVvnlygPSEDTTYST---VAPVPP----GASGEVSIGRPLANTQAYVLDRALQPVPLGVP 294
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 462 GNLVIldSWPGQARTLFGD----HDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMV 537
Cdd:cd12115 295 GELYI--GGAGVARGYLGRpgltAERFLPDPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALR 372
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 538 AHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPseaLRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12115 373 SIPGVREAVVVAIGDAAGERRLVAYIVAEPGAAG---LVEDLRRHLGTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
104-615 |
3.46e-36 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 142.04 E-value: 3.46e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd12116 9 DDRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYILEDAEPALV 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITADE---GVRGGKRTPLKANVDDALTNPEtssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcAPKEMGAEDPLF 260
Cdd:cd12116 89 LTDDAlpdRLPAGLPVLLLALAAAAAAPAA--------------------------------------PRTPVSPDDLAY 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 261 ILYTSGSTGKPKGVlHTTGGYLVYASLTHERVFDYRPGEvywctadiGWVTGHTYI-------VYGPLANGATTILFEGV 333
Cdd:cd12116 131 VIYTSGSTGRPKGV-VVSHRNLVNFLHSMRERLGLGPGD--------RLLAVTTYAfdislleLLLPLLAGARVVIAPRE 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 334 PNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGadgssLRLL-GsvGEPINPEAWQWYYETVGQSrcpivdt 412
Cdd:cd12116 202 TQR-DPEALARLIEAHSITVMQATPATWRMLLDAGWQGRAG-----LTALcG--GEALPPDLAARLLSRVGSL------- 266
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 413 wWQ----TETgaclmTPLPGAHAMKPGSAA----KPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHD-- 482
Cdd:cd12116 267 -WNlygpTET-----TIWSTAARVTAAAGPipigRPLANTQVYVLDAALRPVPPGVPGELYI--GGDGVAQGYLGRPAlt 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 483 --RFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGmQHDIKGQGI 559
Cdd:cd12116 339 aeRFVPDPFAGPGSrLYRTGDLVRRRADGRLEYLGRADGQVKIRGHRIELGEIEAALAAHPGVAQAAVVV-REDGGDRRL 417
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 560 YVYVTLNSGVEPS-EALRQELKQ-----WVrreigpiatPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12116 418 VAYVVLKAGAAPDaAALRAHLRAtlpayMV---------PSAFVRLDALPLTANGKLDRKAL 470
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
91-615 |
1.12e-35 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 140.72 E-value: 1.12e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIwegdDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVfggfSPEA 170
Cdd:cd05923 16 DACAIA----DPARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALI----NPRL 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 171 LAGRIIDC-----KSKVVITAD----EGVRGGKRTPLKANVDDALTNPEtssvqkiivckrtgaeikwnqhrdvwyddlm 241
Cdd:cd05923 88 KAAELAELiergeMTAAVIAVDaqvmDAIFQSGVRVLALSDLVGLGEPE------------------------------- 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 kVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGV-------------LHTTGGYLvYASltHERVFDYRPgevywctadIG 308
Cdd:cd05923 137 -SAGPLIEDPPREPEQPAFVFYTSGTTGLPKGAvipqraaesrvlfMSTQAGLR-HGR--HNVVLGLMP---------LY 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 309 WVTGHTYIVYGPLANGATTIlfegVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGE 388
Cdd:cd05923 204 HVIGFFAVLVAALALDGTYV----VVEEFDPADALKLIEQERVTSLFATPTHLDALAAA--AEFAGLKLSSLRHVTFAGA 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 389 PINpeawQWYYETVGQSR-CPIVDTWWQTETGACLMTPLPgahamKPGSAAKPFFGVVPALVDNLGNLIEGAA---EGNL 464
Cdd:cd05923 278 TMP----DAVLERVNQHLpGEKVNIYGTTEAMNSLYMRDA-----RTGTEMRPGFFSEVRIVRIGGSPDEALAngeEGEL 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 465 VILDSwpgQARTLFGDHDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAE 544
Cdd:cd05923 349 IVAAA---ADAAFTGYLNQPEATAKKLQDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTE 425
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 545 AAVVGMQHDIKGQGIYVYVTLNSGvEPSEalrQELKQWVR-REIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05923 426 VVVIGVADERWGQSVTACVVPREG-TLSA---DELDQFCRaSELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
107-615 |
1.21e-35 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 139.54 E-value: 1.21e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 107 EITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITa 186
Cdd:cd05935 1 SLTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVV- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 degvrggkrtplkanvddaltnpetssvqkiivckrtGAEIkwnqhrdvwyDDLMkvagstcapkemgaedplFILYTSG 266
Cdd:cd05935 80 -------------------------------------GSEL----------DDLA------------------LIPYTSG 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 267 STGKPKGVLHTTGGYLVYASLThERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVpnypDVTRVAKII 346
Cdd:cd05935 95 TTGLPKGCMHTHFSAAANALQS-AVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARW----DRETALELI 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 347 DKHKVNILYTAPTAIRAMMAEGKaaVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDTWWQTETgaCLMTPL 426
Cdd:cd05935 170 EKYKVTFWTNIPTMLVDLLATPE--FKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTGLR---FVEGYGLTET--MSQTHT 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 427 PGAHAMKPGSAAKPFFGVVPALVD-NLGNLIEGAAEGNLVIldSWPgQARTLFGDHDRFVDTYFKTFKGMYF--TGDGAR 503
Cdd:cd05935 243 NPPLRPKLQCLGIP*FGVDARVIDiETGRELPPNEVGEIVV--RGP-QIFKGYWNRPEETEESFIEIKGRRFfrTGDLGY 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEpSEALRQELKQWV 583
Cdd:cd05935 320 MDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVLRPEYR-GKVTEEDIIEWA 398
|
490 500 510
....*....|....*....|....*....|..
gi 15596084 584 RREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05935 399 REQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
84-618 |
4.90e-35 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 139.68 E-value: 4.90e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHLAERG-DQVAIIwegdDpaDHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVV 162
Cdd:PRK07788 56 AHAARRApDRAALI----D--ERGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILL 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 163 FGGFSPEALAGRIIDCKSKVVITADEgvrggkRTPLkanVDDAltnPETSSVQKIIVCKRTGAEIKWNQHRDVwyDDLMK 242
Cdd:PRK07788 130 NTGFSGPQLAEVAAREGVKALVYDDE------FTDL---LSAL---PPDLGRLRAWGGNPDDDEPSGSTDETL--DDLIA 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 243 VAGSTCAPKemgAEDP-LFILYTSGSTGKPKGVLH-TTGGYLVYASLThERVfDYRPGEVYWCTADIGWVTGHTYIVYGp 320
Cdd:PRK07788 196 GSSTAPLPK---PPKPgGIVILTSGTTGTPKGAPRpEPSPLAPLAGLL-SRV-PFRAGETTLLPAPMFHATGWAHLTLA- 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 321 LANGATTIL---FegvpnypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQW 397
Cdd:PRK07788 270 MALGSTVVLrrrF-------DPEATLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSALSPELATR 342
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 398 YYETVGqsrcPIV-DTWWQTETGACLMTPlPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQART 476
Cdd:PRK07788 343 ALEAFG----PVLyNLYGSTEVAFATIAT-PEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNGFPFEGYT 417
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 477 LFGDHDRfVDtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKG 556
Cdd:PRK07788 418 DGRDKQI-ID-------GLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVIGVDDEEFG 489
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15596084 557 QGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATP-DVIqWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK07788 490 QRLRAFVVKAPGAALDE---DAIKDYVRDNLARYKVPrDVV-FLDELPRNPTGKVLKRELREM 548
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
263-621 |
7.58e-35 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 135.69 E-value: 7.58e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 263 YTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATtILFEGVPNYPDVTRV 342
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNGSVVTLLTPLASGAH-VVLAGPAGYRNPGLF 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 343 A---KIIDKHKVNILYTAPTAIRAMMAegkaAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETg 419
Cdd:cd05944 87 DnfwKLVERYRITSLSTVPTVYAALLQ----VPVNADISSLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTEA- 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 420 ACLMTPLPGAHAMKPGSA--AKPFFGVVPALVDNLGNLIEGAAEGNL-VILDSWPGQAR-TLFGDHDRFVDTYfktfKGM 495
Cdd:cd05944 159 TCLVAVNPPDGPKRPGSVglRLPYARVRIKVLDGVGRLLRDCAPDEVgEICVAGPGVFGgYLYTEGNKNAFVA----DGW 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 496 YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAl 575
Cdd:cd05944 235 LNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGAVVEEE- 313
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 15596084 576 rqELKQWVRREIGP-IATPDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:cd05944 314 --ELLAWARDHVPErAAVPKHIEVLEELPVTAVGKVFKPALRADAIH 358
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
106-616 |
3.99e-34 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 136.57 E-value: 3.99e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK08276 10 EVVTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 aDEGVRggkrtPLKANVDDALTNPETssvqkiiVCKRTGAEIkwNQHRDvwYDDLmkVAGstcAPKEMGAEDPL--FILY 263
Cdd:PRK08276 90 -SAALA-----DTAAELAAELPAGVP-------LLLVVAGPV--PGFRS--YEEA--LAA---QPDTPIADETAgaDMLY 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHT-TGGYLVYASLTHERVFDYR----PGEVYWCTADIGwvtgHTY-IVYG--PLANGATTILFEGVpn 335
Cdd:PRK08276 148 SSGTTGRPKGIKRPlPGLDPDEAPGMMLALLGFGmyggPDSVYLSPAPLY----HTApLRFGmsALALGGTVVVMEKF-- 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 336 ypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrcPIVD-TWW 414
Cdd:PRK08276 222 --DAEEALALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAIHAAAPCPVEVKRAMIDWWG----PIIHeYYA 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 QTET-GACLMTPlpGAHAMKPGSAAKPFFGVVPALVDNlGNLIEGAAEGNlvILDSWPGQARTLFGDHDRFVDTYFKtfK 493
Cdd:PRK08276 296 SSEGgGVTVITS--EDWLAHPGSVGKAVLGEVRILDED-GNELPPGEIGT--VYFEMDGYPFEYHNDPEKTAAARNP--H 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 494 GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSE 573
Cdd:PRK08276 369 GWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKVADVAVFGVPDEEMGERVKAVVQPADGADAGD 448
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 15596084 574 ALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK08276 449 ALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
82-617 |
8.39e-34 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 135.84 E-value: 8.39e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGDDPADHQeiTYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:cd12119 2 LEHAARLHGDREIVSRTHEGEVHRY--TYAEVAERARRLANALRRLGVKPGDRVATLAWNTHRHLELYYAVPGMGAVLHT 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADEGVrggkrtPLKANVDDALTNPETssvqkiIVCKRTGAEIKWNQHRDVW-YDDL 240
Cdd:cd12119 80 INPRLFPEQIAYIINHAEDRVVFVDRDFL------PLLEAIAPRLPTVEH------VVVMTDDAAMPEPAGVGVLaYEEL 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 241 MKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGV--------LHTTGGYLVYASLTHERvfdyrpgEVY----------- 301
Cdd:cd12119 148 LAAESPEYDWPDFDENTAAAICYTSGTTGNPKGVvyshrslvLHAMAALLTDGLGLSES-------DVVlpvvpmfhvna 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 302 WCTADIGWVTGHTYIVYGPLANGATtilfegvpnypdvtrVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLR 381
Cdd:cd12119 221 WGLPYAAAMVGAKLVLPGPYLDPAS---------------LAELIEREGVTFAAGVPTVWQGLLDHLEAN--GRDLSSLR 283
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 382 LLGSVGEPINPEAWQWY-YETVgqsrcPIVDTWWQTETGACLMTPLPGAHAMKPGSAAK---------PFFGVVPALVDN 451
Cdd:cd12119 284 RVVIGGSAVPRSLIEAFeERGV-----RVIHAWGMTETSPLGTVARPPSEHSNLSEDEQlalrakqgrPVPGVELRIVDD 358
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 452 LGNLIE--GAAEGNLVILDSWpgQARTLFGDHDR----FVDTYFKTfkgmyftGDGARRDEDGYYWITGRVDDVLNVSGH 525
Cdd:cd12119 359 DGRELPwdGKAVGELQVRGPW--VTKSYYKNDEEsealTEDGWLRT-------GDVATIDEDGYLTITDRSKDVIKSGGE 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 526 RMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKT 605
Cdd:cd12119 430 WISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGATVTA---EELLEFLADKVAKWWLPDDVVFVDEIPKT 506
|
570
....*....|..
gi 15596084 606 RSGKIMRRILRK 617
Cdd:cd12119 507 STGKIDKKALRE 518
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
90-622 |
8.86e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 135.78 E-value: 8.86e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 90 GDQVAIIWeGDdpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPE 169
Cdd:PRK07798 17 PDRVALVC-GD-----RRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPVNVNYRYVED 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 170 ALAGRIIDCKSKVVITADEgvrggkrtpLKANVDDALtnPETSSVQKIIVCK-------RTGAeikwnqhrdVWYDDLMK 242
Cdd:PRK07798 91 ELRYLLDDSDAVALVYERE---------FAPRVAEVL--PRLPKLRTLVVVEdgsgndlLPGA---------VDYEDALA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 243 VAGSTCAPKEMGAEDpLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWV-TGHTYIVYGPL 321
Cdd:PRK07798 151 AGSPERDFGERSPDD-LYLLYTGGTTGMPKGVMWRQED-IFRVLLGGRDFATGEPIEDEEELAKRAAAgPGMRRFPAPPL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 ANGATTI-----LFEG--VPNYPDVT----RVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPI 390
Cdd:PRK07798 229 MHGAGQWaafaaLFSGqtVVLLPDVRfdadEVWRTIEREKVNVITIVGDAMARPLLDALEARGPYDLSSLFAIASGGALF 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 391 NPEAWQWYYETVGQSRcpIVDTWWQTETGACLMtplpGAHAMKPGSAAKPFFGVVP--ALVDNLGNLIE-GAAEGNLVil 467
Cdd:PRK07798 309 SPSVKEALLELLPNVV--LTDSIGSSETGFGGS----GTVAKGAVHTGGPRFTIGPrtVVLDEDGNPVEpGSGEIGWI-- 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 dswpgqART------LFGDHDRFVDTyFKTFKGM-Y-FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAH 539
Cdd:PRK07798 381 ------ARRghiplgYYKDPEKTAET-FPTIDGVrYaIPGDRARVEADGTITLLGRGSVCINTGGEKVFPEEVEEALKAH 453
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 540 PKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK07798 454 PDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQRSPAGKADYRWAKEQA 530
|
...
gi 15596084 620 AAE 622
Cdd:PRK07798 531 AER 533
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
84-616 |
1.35e-33 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 135.11 E-value: 1.35e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHLAERGDQVAIIWEGDdpadhqEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACT----RIGAIH 159
Cdd:PRK06188 20 SALKRYPDRPALVLGDT------RLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQlaglRRTALH 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 160 SVVfggfSPEALAGRIIDCKSKVVITadEGVRGGKRT-PLKANVDdaltnpetsSVQKIIVCKRTGAEikwnqhRDVWyd 238
Cdd:PRK06188 94 PLG----SLDDHAYVLEDAGISTLIV--DPAPFVERAlALLARVP---------SLKHVLTLGPVPDG------VDLL-- 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 dlmkVAGSTCAPKEMGAE----DPLFILYTSGSTGKPKGVLHTTGGYLVYASlthervfdyrpgevyWCTADIGWVTGHT 314
Cdd:PRK06188 151 ----AAAAKFGPAPLVAAalppDIAGLAYTGGTTGKPKGVMGTHRSIATMAQ---------------IQLAEWEWPADPR 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 315 YIVYGPLAN------------GATTILFEGVpnypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGkaAVAGADGSSLRL 382
Cdd:PRK06188 212 FLMCTPLSHaggafflptllrGGTVIVLAKF----DPAEVLRAIEEQRITATFLVPTMIYALLDHP--DLRTRDLSSLET 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 383 LGSVGEPINPEAWQWYYETVGqsrcPI-VDTWWQTETGACLMTPLPGAHAMKP----GSAAKPFFGVVPALVDNLGNLI- 456
Cdd:PRK06188 286 VYYGASPMSPVRLAEAIERFG----PIfAQYYGQTEAPMVITYLRKRDHDPDDpkrlTSCGRPTPGLRVALLDEDGREVa 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 457 -----EGAAEGNLVILDSWPGQARTLfgdhdrfvdtyfKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTA 530
Cdd:PRK06188 362 qgevgEICVRGPLVMDGYWNRPEETA------------EAFRDGWLhTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFPR 429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 531 EVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKI 610
Cdd:PRK06188 430 EVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGSVHAPKQVDFVDSLPLTALGKP 506
|
....*.
gi 15596084 611 MRRILR 616
Cdd:PRK06188 507 DKKALR 512
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
82-553 |
3.92e-32 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 131.76 E-value: 3.92e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGDDpaDHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:COG1022 17 LRRRAARFPDRVALREKEDG--IWQSLTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVP 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADEGVRggkrtplkANVDDALtnPETSSVQKIIVCKRTGAeikWNQHRDVWYDDLM 241
Cdd:COG1022 95 IYPTSSAEEVAYILNDSGAKVLFVEDQEQL--------DKLLEVR--DELPSLRHIVVLDPRGL---RDDPRLLSLDELL 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 KVAGSTCAPKEM-------GAEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVY-----WCtadigW 309
Cdd:COG1022 162 ALGREVADPAELearraavKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGDRTlsflpLA-----H 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 310 VTGHTYIVYGpLANGATTilfegvpNYP-DVTRVAKIIDKHKVNILYTAP-------TAIRAMMAEG------------- 368
Cdd:COG1022 236 VFERTVSYYA-LAAGATV-------AFAeSPDTLAEDLREVKPTFMLAVPrvwekvyAGIQAKAEEAgglkrklfrwala 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 369 ------KAAVAG-------------AD-----------GSSLRLLGSVGEPINPEAwQWYYETVGqsrCPIVDTWWQTET 418
Cdd:COG1022 308 vgrryaRARLAGkspslllrlkhalADklvfsklrealGGRLRFAVSGGAALGPEL-ARFFRALG---IPVLEGYGLTET 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 419 GA--CLMTPlpgaHAMKPGSAAKPFFGVVPALvdnlgnliegAAEGnlVILdswpgqARtlfGDHdrfvdtyfkTFKGmY 496
Cdd:COG1022 384 SPviTVNRP----GDNRIGTVGPPLPGVEVKI----------AEDG--EIL------VR---GPN---------VMKG-Y 428
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 497 F-----------------TGDGARRDEDGYYWITGRVDDVLNVSGhrmGT----AEVESAMVAHPKVAEAAVVGMQHD 553
Cdd:COG1022 429 YknpeataeafdadgwlhTGDIGELDEDGFLRITGRKKDLIVTSG---GKnvapQPIENALKASPLIEQAVVVGDGRP 503
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
106-615 |
8.76e-32 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 130.15 E-value: 8.76e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK06710 48 KDITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILC 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 AD------EGVRGGKRTP--LKANVDDALTNPEtSSVQKIIVCKRTGAEIK---------WN---QHRDVWYDDLmkvag 245
Cdd:PRK06710 128 LDlvfprvTNVQSATKIEhvIVTRIADFLPFPK-NLLYPFVQKKQSNLVVKvsesetihlWNsveKEVNTGVEVP----- 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 246 stCAPKEmgaeDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGE-VYWCTADIGWVTGHTYIVYGPLANG 324
Cdd:PRK06710 202 --CDPEN----DLALLQYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNCKEGEeVVLGVLPFFHVYGMTAVMNLSIMQG 275
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 325 ATTILfegVPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMaeGKAAVAGADGSSLRLLGSVGEPInPEAWQWYYETVGQ 404
Cdd:PRK06710 276 YKMVL---IPKF-DMKMVFEAIKKHKVTLFPGAPTIYIALL--NSPLLKEYDISSIRACISGSAPL-PVEVQEKFETVTG 348
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 405 SR----------CPIVDT--WWQTETGACLMTPLPGAHAM----KPGSAAKPffgvvpalvdnlGNLIEGAAEGNLVILD 468
Cdd:PRK06710 349 GKlvegygltesSPVTHSnfLWEKRVPGSIGVPWPDTEAMimslETGEALPP------------GEIGEIVVKGPQIMKG 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 469 SW--PGQARTLFGDhdrfvdtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAA 546
Cdd:PRK06710 417 YWnkPEETAAVLQD-------------GWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVV 483
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15596084 547 VVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK06710 484 TIGVPDPYRGETVKAFVVLKEGTECSE---EELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVL 549
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
83-616 |
9.59e-32 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 129.43 E-value: 9.59e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 83 DRHLAERGDQVAIIWEGDDPAdhqeITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVV 162
Cdd:PRK13391 4 GIHAQTTPDKPAVIMASTGEV----VTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCV 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 163 FGGFSPEALAGRIIDCKSKVVITadegvrggkrTPLKANVDDALTNpETSSVQKIIVCKRTGAEIKWnqhrdVWYDDLMK 242
Cdd:PRK13391 80 NSHLTPAEAAYIVDDSGARALIT----------SAAKLDVARALLK-QCPGVRHRLVLDGDGELEGF-----VGYAEAVA 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 243 VAGSTCAPKEMGAEDplfILYTSGSTGKPKGVL---------HTTGgylvyasLTH--ERVFDYRPGEVYWCTADIGwvt 311
Cdd:PRK13391 144 GLPATPIADESLGTD---MLYSSGTTGRPKGIKrplpeqppdTPLP-------LTAflQRLWGFRSDMVYLSPAPLY--- 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 312 gHTyivyGPLA-------NGATTILFEGVpnypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLG 384
Cdd:PRK13391 211 -HS----APQRavmlvirLGGTVIVMEHF----DAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSSLEVAI 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 385 SVGEPINPEAWQWYYETVGqsrcPIVDTWW-QTE-TGACLMTP---LpgAHamkPGSAAKPFFGVVPALVDNLGNLIEGA 459
Cdd:PRK13391 282 HAAAPCPPQVKEQMIDWWG----PIIHEYYaATEgLGFTACDSeewL--AH---PGTVGRAMFGDLHILDDDGAELPPGE 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 460 aegnlvildswPGQ---------------ARTLFGDHDRfvdtyfktfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSG 524
Cdd:PRK13391 353 -----------PGTiwfeggrpfeylndpAKTAEARHPD---------GTWSTVGDIGYVDEDGYLYLTDRAAFMIISGG 412
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 525 HRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPK 604
Cdd:PRK13391 413 VNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVVQPVDGVDPGPALAAELIAFCRQRLSRQKCPRSIDFEDELPR 492
|
570
....*....|..
gi 15596084 605 TRSGKIMRRILR 616
Cdd:PRK13391 493 LPTGKLYKRLLR 504
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
91-618 |
1.05e-31 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 128.93 E-value: 1.05e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsVVF--GGFSP 168
Cdd:PRK03640 17 DRTAIEFEE------KKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAV--AVLlnTRLSR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 169 EALAGRIIDCKSKVVITADEgvrggkrtplkanvddaltnpetsSVQKIIVCKRtgaeikwnqhrdVWYDDLMKVAGSTC 248
Cdd:PRK03640 89 EELLWQLDDAEVKCLITDDD------------------------FEAKLIPGIS------------VKFAELMNGPKEEA 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 249 APKEMGAEDPLF-ILYTSGSTGKPKGVLHTTGGYL---VYASLT---HERvfdyrpgEVYWCTADIGWVTGHTyIVYGPL 321
Cdd:PRK03640 133 EIQEEFDLDEVAtIMYTSGTTGKPKGVIQTYGNHWwsaVGSALNlglTED-------DCWLAAVPIFHISGLS-ILMRSV 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 ANGATTILfegVPNYpDVTRVAKIIDKHKVNILYTAPTairamMAEGKAAVAGADG--SSLR--LLGsvGEPINPEAwqw 397
Cdd:PRK03640 205 IYGMRVVL---VEKF-DAEKINKLLQTGGVTIISVVST-----MLQRLLERLGEGTypSSFRcmLLG--GGPAPKPL--- 270
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 398 yYETVGQSRCPIVDTWWQTETGACLMTpLPGAHAM-KPGSAAKPFFGVVPALVDNlGNLIEGAAEGNLVIldswpgQART 476
Cdd:PRK03640 271 -LEQCKEKGIPVYQSYGMTETASQIVT-LSPEDALtKLGSAGKPLFPCELKIEKD-GVVVPPFEEGEIVV------KGPN 341
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 477 LFG--------DHDRFVDTYFKTfkgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVV 548
Cdd:PRK03640 342 VTKgylnredaTRETFQDGWFKT-------GDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPGVAEAGVV 414
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 549 GMQHDIKGQGIYVYVTlnSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK03640 415 GVPDDKWGQVPVAFVV--KSGEVTEE---ELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELKQL 479
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
89-618 |
2.32e-31 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 128.05 E-value: 2.32e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 89 RGDQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFS 167
Cdd:PRK06839 15 HPDRIAII------TEEEEMTYKQLHEYVSKVAAYLIYElNVKKGERIAILSQNSLEYIVLLFAIAKVECIAVPLNIRLT 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 168 PEALAGRIIDCKSKVVITADEgvrggkrtplKANVDDALTnpetssvqkiivcKRTGAEikwnqhRDVWYDDLMKVAGST 247
Cdd:PRK06839 89 ENELIFQLKDSGTTVLFVEKT----------FQNMALSMQ-------------KVSYVQ------RVISITSLKEIEDRK 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 248 CAPKEMGAED-PLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGAT 326
Cdd:PRK06839 140 IDNFVEKNESaSFIICYTSGTTGKPKGAVLTQEN-MFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAFPTLFAGGV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 327 TIlfegVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAgaDGSSLRLLGSVGEPINPEAWQWYYET---VG 403
Cdd:PRK06839 219 II----VPRKFEPTKALSMIEKHKVTVVMGVPTIHQALINCSKFETT--NLQSVRWFYNGGAPCPEELMREFIDRgflFG 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 404 QSrcpivdtWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHDR 483
Cdd:PRK06839 293 QG-------FGMTETSPTVFMLSEEDARRKVGSIGKPVLFCDYELIDENKNKVEVGEVGELLI--RGPNVMKEYWNRPDA 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 484 FVDTyfkTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYV 563
Cdd:PRK06839 364 TEET---IQDGWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVYEVAVVGRQHVKWGEIPIAFI 440
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 564 TLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK06839 441 VKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
82-621 |
6.13e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 127.55 E-value: 6.13e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:PRK06164 16 LDAHARARPDAVALIDED------RPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADeGVRGGKRTPLKANVDDALTNPetssVQKIIVCKRTGAEIKwNQHRDVWYD--D 239
Cdd:PRK06164 90 VNTRYRSHEVAHILGRGRARWLVVWP-GFKGIDFAAILAAVPPDALPP----LRAIAVVDDAADATP-APAPGARVQlfA 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 240 LMKVAGSTCAPKEMGAEDPLFILY-TSGSTGKPKGVLHTTGGYLVYASLThERVFDYRPGEVYWCTADIGWVTGHTYIVy 318
Cdd:PRK06164 164 LPDPAPPAAAGERAADPDAGALLFtTSGTTSGPKLVLHRQATLLRHARAI-ARAYGYDPGAVLLAALPFCGVFGFSTLL- 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 319 GPLANGATTILFEGVpnypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvagADGSSLRLLGSVGepINPeAWQWY 398
Cdd:PRK06164 242 GALAGGAPLVCEPVF----DAARTARALRRHRVTHTFGNDEMLRRILDTAGER---ADFPSARLFGFAS--FAP-ALGEL 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 399 YETVGQSRCPIVDTWWQTETGAclmtpLPGAHAMKPGSAAKPFFGVVPA-------LVDNL-GNLIEGAAEGNLVIldSW 470
Cdd:PRK06164 312 AALARARGVPLTGLYGSSEVQA-----LVALQPATDPVSVRIEGGGRPAspearvrARDPQdGALLPDGESGEIEI--RA 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 471 PGQARTLFGDHDRFVDTYfkTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGM 550
Cdd:PRK06164 385 PSLMRGYLDNPDATARAL--TDDGYFRTGDLGYTRGDGQFVYQTRMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGA 462
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 551 QHDikGQGI-YVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSG---KIMRRILRKIAAA 621
Cdd:PRK06164 463 TRD--GKTVpVAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMAQA 532
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
104-549 |
1.27e-30 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 125.40 E-value: 1.27e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd05907 2 VWQPITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKAL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITADegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgAEDPLFILY 263
Cdd:cd05907 82 FVED-------------------------------------------------------------------PDDLATIIY 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYPDVTRVA 343
Cdd:cd05907 95 TSGTTGRPKGVMLSHRN-ILSNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASSAETLLDDLSEV 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 344 K---------IIDKHKVNILYTAPTAIRAMMAEgKAAvagadGSSLRLLGSVGEPINPEAWQWyYETVGqsrCPIVDTWW 414
Cdd:cd05907 174 RptvflavprVWEKVYAAIKVKAVPGLKRKLFD-LAV-----GGRLRFAASGGAPLPAELLHF-FRALG---IPVYEGYG 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 QTETGA--CLMTPlpgaHAMKPGSAAKPFFGVVPALVDNlGNLIegaAEGNLVILDSW--PGQARTLFgDHDrfvdtyfk 490
Cdd:cd05907 244 LTETSAvvTLNPP----GDNRIGTVGKPLPGVEVRIADD-GEIL---VRGPNVMLGYYknPEATAEAL-DAD-------- 306
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 491 tfkGMYFTGDGARRDEDGYYWITGRVDDVL-NVSGHRMGTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd05907 307 ---GWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
117-616 |
3.46e-30 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 124.09 E-value: 3.46e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 117 VCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVF----GGFSPEALAGRIIDCKSKVVItADEGVRG 192
Cdd:cd05922 3 VSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFvplnPTLKESVLRYLVADAGGRIVL-ADAGAAD 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 193 GKRTPLKANVDDaltnpetssvqkiivckrtgaeikwnqhrDVWYD-DLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKP 271
Cdd:cd05922 82 RLRDALPASPDP-----------------------------GTVLDaDGIRAARASAPAHEVSHEDLALLLYTSGSTGSP 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 272 KGVL--HTTggyLVYASLTHERVFDYRPGEVYWCTADIGWVTGhTYIVYGPLANGATTILFEGvpNYPDVTrVAKIIDKH 349
Cdd:cd05922 133 KLVRlsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYG-LSVLNTHLLRGATLVLTND--GVLDDA-FWEDLREH 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 350 KVNILYTAPTaIRAMMAEGKAAVAGAdgSSLRLLGSVGEPINPEAWQWYYETVGQSRcpIVDTWWQTETGACLMTPLPGA 429
Cdd:cd05922 206 GATGLAGVPS-TYAMLTRLGFDPAKL--PSLRYLTQAGGRLPQETIARLRELLPGAQ--VYVMYGQTEATRRMTYLPPER 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 430 HAMKPGSAAKPFFGVVPALVDNLGN------LIEGAAEGNLVILDSWpgqartlfgdHDRFVDTYFKTFKGMYFTGDGAR 503
Cdd:cd05922 281 ILEKPGSIGLAIPGGEFEILDDDGTptppgePGEIVHRGPNVMKGYW----------NDPPYRRKEGRGGGVLHTGDLAR 350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 504 RDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQhDIKGQGIYVYVTLNSGVEPSEALRqelkqwV 583
Cdd:cd05922 351 RDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLP-DPLGEKLALFVTAPDKIDPKDVLR------S 423
|
490 500 510
....*....|....*....|....*....|....
gi 15596084 584 RREIGPI-ATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05922 424 LAERLPPyKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
82-617 |
5.24e-30 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 123.95 E-value: 5.24e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEgddpadHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:cd12118 10 LERAAAVYPDRTSIVYG------DRRYTWRQTYDRCRRLASALAALGISRGDTVAVLAPNTPAMYELHFGVPMAGAVLNA 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVItadegvrggkrtplkanVDDALTnpetssvqkiivckrtgaeikwnqhrdvwYDDLM 241
Cdd:cd12118 84 LNTRLDAEEIAFILRHSEAKVLF-----------------VDREFE-----------------------------YEDLL 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 KvAGSTCAPKEMGAE--DPLFILYTSGSTGKPKGVLHT-TGGYLvyASLTHERVFDYRPGEVYWCTADIGWVTGHTYIvY 318
Cdd:cd12118 118 A-EGDPDFEWIPPADewDPIALNYTSGTTGRPKGVVYHhRGAYL--NALANILEWEMKQHPVYLWTLPMFHCNGWCFP-W 193
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 319 GPLANGATTILFEGVpnypDVTRVAKIIDKHKVNILYTAPTaIRAMMAEGKAA------------VAGADGSSlRLLGSV 386
Cdd:cd12118 194 TVAAVGGTNVCLRKV----DAKAIYDLIEKHKVTHFCGAPT-VLNMLANAPPSdarplphrvhvmTAGAPPPA-AVLAKM 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 387 gEPINPEAWQWY--YETVGqsrcPIVDTWWQTETGAclmTPLPGAHAMKP--GSAAKPFFGV----------VPALVDNL 452
Cdd:cd12118 268 -EELGFDVTHVYglTETYG----PATVCAWKPEWDE---LPTEERARLKArqGVRYVGLEEVdvldpetmkpVPRDGKTI 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 453 GnliEGAAEGNLVILDswpgqartlfgdhdrfvdtYFK-------TFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSG 524
Cdd:cd12118 340 G---EIVFRGNIVMKG-------------------YLKnpeataeAFRGGWFhSGDLAVIHPDGYIEIKDRSKDIIISGG 397
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 525 HRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPgLPK 604
Cdd:cd12118 398 ENISSVEVEGVLYKHPAVLEAAVVARPDEKWGEVPCAFVELKEGAKVTE---EEIIAFCREHLAGFMVPKTVVFGE-LPK 473
|
570
....*....|...
gi 15596084 605 TRSGKIMRRILRK 617
Cdd:cd12118 474 TSTGKIQKFVLRD 486
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
82-617 |
7.09e-30 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 124.73 E-value: 7.09e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsV 161
Cdd:PRK05605 38 YDNAVARFGDRPALDFFG------ATTTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAV--V 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGG--FSPEALAGRIIDCKSKVVITAD------EGVRGgkRTPLK----ANVDDALTNPETSSVQ---KIIVCKR---T 223
Cdd:PRK05605 110 VEHNplYTAHELEHPFEDHGARVAIVWDkvaptvERLRR--TTPLEtivsVNMIAAMPLLQRLALRlpiPALRKARaalT 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 224 GAE---IKWNQhrdvWYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGvlhttggylvyASLTHERVFdyrpgev 300
Cdd:PRK05605 188 GPApgtVPWET----LVDAAIGGDGSDVSHPRPTPDDVALILYTSGTTGKPKG-----------AQLTHRNLF------- 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 301 ywCTADIG--WVTG---------------HTY-----IVYGPLAnGATTILFegvPNyPDVTRVAKIIDKHKVNILYTAP 358
Cdd:PRK05605 246 --ANAAQGkaWVPGlgdgpervlaalpmfHAYgltlcLTLAVSI-GGELVLL---PA-PDIDLILDAMKKHPPTWLPGVP 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 359 TAIRAMMAEgkAAVAGADGSSLR--LLGSVGEPInpeawqwyyETVGQsrcpivdtwWQTETGACL-----MT---PL-- 426
Cdd:PRK05605 319 PLYEKIAEA--AEERGVDLSGVRnaFSGAMALPV---------STVEL---------WEKLTGGLLvegygLTetsPIiv 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 427 --PGAHAMKPGSAAKPFFGVVPALVD--NLGNLIEGAAEGNLVIldswpgQARTLFGDHDRFVDTYFKTFKGMYF-TGDG 501
Cdd:PRK05605 379 gnPMSDDRRPGYVGVPFPDTEVRIVDpeDPDETMPDGEEGELLV------RGPQVFKGYWNRPEETAKSFLDGWFrTGDV 452
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 502 ARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQ 581
Cdd:PRK05605 453 VVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPE---GLRA 529
|
570 580 590
....*....|....*....|....*....|....*.
gi 15596084 582 WVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK05605 530 YCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVRE 565
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
75-617 |
8.08e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 124.00 E-value: 8.08e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 75 LNVSHnCLDRHLAERGDQVAIIWeGDdpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTR 154
Cdd:PRK07470 7 MNLAH-FLRQAARRFPDRIALVW-GD-----RSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 155 IGAIHSVVFGGFSPEALAGriidckskvvITADEGVRGgkrtpLKANVDdaltNPETSSVqkiivCKRTGAEIKW----- 229
Cdd:PRK07470 80 LGAVWVPTNFRQTPDEVAY----------LAEASGARA-----MICHAD----FPEHAAA-----VRAASPDLTHvvaig 135
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 230 --NQHRDvwYDDLMKV-AGSTCAPKEMGAEDPLFILYTSGSTGKPK-GVL-HTTGGYLVYASLThervfDYRPGevywct 304
Cdd:PRK07470 136 gaRAGLD--YEALVARhLGARVANAAVDHDDPCWFFFTSGTTGRPKaAVLtHGQMAFVITNHLA-----DLMPG------ 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 305 adigwVTGH-TYIVYGPL------------ANGATTILFEGVPNypDVTRVAKIIDKHKVNILYTAPTaIRAMMAEgKAA 371
Cdd:PRK07470 203 -----TTEQdASLVVAPLshgagihqlcqvARGAATVLLPSERF--DPAEVWALVERHRVTNLFTVPT-ILKMLVE-HPA 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 372 VAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDTWWQTE-TGAclMTPLPGA-HAMKPGSAAK------PFFG 443
Cdd:PRK07470 274 VDRYDHSSLRYVIYAGAPMYRADQKRALAKLGKV---LVQYFGLGEvTGN--ITVLPPAlHDAEDGPDARigtcgfERTG 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 444 VVPALVDNLGNLIEGAAEGNLVILdswpGQArtLFGDHDRFVDTYFKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNV 522
Cdd:PRK07470 349 MEVQIQDDEGRELPPGETGEICVI----GPA--VFAGYYNNPEANAKAFRDGWFrTGDLGHLDARGFLYITGRASDMYIS 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 523 SGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQ-GIYVYVtLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPG 601
Cdd:PRK07470 423 GGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEvGVAVCV-ARDGAPVDEA---ELLAWLDGKVARYKLPKRFFFWDA 498
|
570
....*....|....*.
gi 15596084 602 LPKTRSGKIMRRILRK 617
Cdd:PRK07470 499 LPKSGYGKITKKMVRE 514
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
82-615 |
9.95e-30 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 123.21 E-value: 9.95e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIwegDDPadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsV 161
Cdd:cd05920 21 LARSAARHPDRIAVV---DGD---RRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV--P 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFggfspeALAGRiidckskvvitadegvrggKRTPLKAnvddaltnpetssvqkiiVCKRTGAE--IKWNQHRDVWYDD 239
Cdd:cd05920 93 VL------ALPSH-------------------RRSELSA------------------FCAHAEAVayIVPDRHAGFDHRA 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 240 LMKVAGSTCApkemgaeDPLFILYTSGSTGKPKGVLHTTGGYlVYASLTHERVFDYRPGEVYWCTADIGwvtgHTYI--- 316
Cdd:cd05920 130 LARELAESIP-------EVALFLLSGGTTGTPKLIPRTHNDY-AYNVRASAEVCGLDQDTVYLAVLPAA----HNFPlac 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 --VYGPLANGATTILfegVPNyPDVTRVAKIIDKHKVNILYTAPTAirAMMAEGKAAVAGADGSSLRLLGSVGEPINPEA 394
Cdd:cd05920 198 pgVLGTLLAGGRVVL---APD-PSPDAAFPLIEREGVTVTALVPAL--VSLWLDAAASRRADLSSLRLLQVGGARLSPAL 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 395 WQWYYETVGqsrCPIVDTWWQTETGACLmTPLPGAHAMKPGSAAKPffgVVP----ALVDNLGNLIEGAAEGNLVildsw 470
Cdd:cd05920 272 ARRVPPVLG---CTLQQVFGMAEGLLNY-TRLDDPDEVIIHTQGRP---MSPddeiRVVDEEGNPVPPGEEGELL----- 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 471 pgqAR---TLFG-----DHDRfvdTYFkTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKV 542
Cdd:cd05920 340 ---TRgpyTIRGyyrapEHNA---RAF-TPDGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAV 412
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15596084 543 AEAAVVGMQHDIKGQGIYVYVTLNsGVEPS-EALRQELKQwvrREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd05920 413 HDAAVVAMPDELLGERSCAFVVLR-DPPPSaAQLRRFLRE---RGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
84-622 |
1.47e-29 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 123.61 E-value: 1.47e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHLA-ERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVV 162
Cdd:PRK06178 40 RAWArERPQRPAIIFYG------HVITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPV 113
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 163 FGGFSPEALAGRIIDCKSKVVITAD------EGVRGGK--RTPLKANVDDALTNPETSSVQKIIVCKRTGAEikwnqhrd 234
Cdd:PRK06178 114 SPLFREHELSYELNDAGAEVLLALDqlapvvEQVRAETslRHVIVTSLADVLPAEPTLPLPDSLRAPRLAAA-------- 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 235 VWYDDLMKVAGSTC-APKEMGAEDPLFIL-YTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTG 312
Cdd:PRK06178 186 GAIDLLPALRACTApVPLPPPALDALAALnYTGGTTGMPKGCEHTQRDMVYTAAAAYAVAVVGGEDSVFLSFLPEFWIAG 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 313 HTYIVYGPLANGATTILFegvpNYPDVTRVAKIIDKHKVNILytaptairAMMAEGKA------AVAGADGSSLRLLGSV 386
Cdd:PRK06178 266 ENFGLLFPLFSGATLVLL----ARWDAVAFMAAVERYRVTRT--------VMLVDNAVelmdhpRFAEYDLSSLRQVRVV 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 387 G--EPINPEAWQWYYETVGqsrCPIVDT-WWQTETGAC-------------LMT-------PLPGAHAMkpgsaakpffg 443
Cdd:PRK06178 334 SfvKKLNPDYRQRWRALTG---SVLAEAaWGMTETHTCdtftagfqdddfdLLSqpvfvglPVPGTEFK----------- 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 444 vvpaLVD-NLGNLIEGAAEGNLVI-----LDSWPGQARTlfgDHDRFVDtyfktfkGMYFTGDGARRDEDGYYWITGRVD 517
Cdd:PRK06178 400 ----ICDfETGELLPLGAEGEIVVrtpslLKGYWNKPEA---TAEALRD-------GWLHTGDIGKIDEQGFLHYLGRRK 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 518 DVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPdVIQ 597
Cdd:PRK06178 466 EMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAVYKVP-EIR 541
|
570 580
....*....|....*....|....*
gi 15596084 598 WAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK06178 542 IVDALPMTATGKVRKQDLQALAEEL 566
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
106-623 |
2.20e-29 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 125.45 E-value: 2.20e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK12316 535 ETLDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLS 614
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 ADegvRGGKRTPLKANVDD-ALTNPetssvqkiivckrtgaeikwnqhrDVWYDdlmkvAGSTCAPK-EMGAEDPLFILY 263
Cdd:PRK12316 615 QS---HLGRKLPLAAGVQVlDLDRP------------------------AAWLE-----GYSEENPGtELNPENLAYVIY 662
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHTTGGYL--------VYASLTHERVFDYRPgevywCTADIGwvtghTYIVYGPLANGATTILfEGVPN 335
Cdd:PRK12316 663 TSGSTGKPKGAGNRHRALSnrlcwmqqAYGLGVGDTVLQKTP-----FSFDVS-----VWEFFWPLMSGARLVV-AAPGD 731
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 336 YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGkaavAGADGSSLRLLGSVGEPINPEAWQWYYETVGQsrCPIVDTWWQ 415
Cdd:PRK12316 732 HRDPAKLVELINREGVDTLHFVPSMLQAFLQDE----DVASCTSLRRIVCSGEALPADAQEQVFAKLPQ--AGLYNLYGP 805
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 416 TET--GACLMTPLPGAHAMKPgsAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSwpGQARTLFG----DHDRFVDTYF 489
Cdd:PRK12316 806 TEAaiDVTHWTCVEEGGDSVP--IGRPIANLACYILDANLEPVPVGVLGELYLAGR--GLARGYHGrpglTAERFVPSPF 881
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 490 KTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQhdikGQGIYVYVTLNSgv 569
Cdd:PRK12316 882 VAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD----GKQLVGYVVLES-- 955
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 570 ePSEALRQELKQWVRREIGPIATPDviQWAP--GLPKTRSGKIMRRILRK----IAAAEY 623
Cdd:PRK12316 956 -EGGDWREALKAHLAASLPEYMVPA--QWLAleRLPLTPNGKLDRKALPApeasVAQQGY 1012
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
104-615 |
9.39e-29 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 119.88 E-value: 9.39e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAgriidckskvV 183
Cdd:cd17650 9 ATRQLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQ----------Y 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITADEGVrggkrtplkanvDDALTNPEtssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgaeDPLFILY 263
Cdd:cd17650 79 MLEDSGA------------KLLLTQPE----------------------------------------------DLAYVIY 100
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVL----HTTGGYLVYASLTHERVFDYRPGEVYWCTADIgwvtghtyiVYGPLA----NGATTILfegVPN 335
Cdd:cd17650 101 TSGTTGKPKGVMvehrNVAHAAHAWRREYELDSFPVRLLQMASFSFDV---------FAGDFArsllNGGTLVI---CPD 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 336 --YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLgSVGEPINPEAW-QWYYETVGQsRCPIVDT 412
Cdd:cd17650 169 evKLDPAALYDLILKSRITLMESTPALIRPVMAY--VYRNGLDLSAMRLL-IVGSDGCKAQDfKTLAARFGQ-GMRIINS 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 413 WWQTEtgACL--------MTPLPGAhAMKPgsAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHD-- 482
Cdd:cd17650 245 YGVTE--ATIdstyyeegRDPLGDS-ANVP--IGRPLPNTAMYVLDERLQPQPVGVAGELYI--GGAGVARGYLNRPElt 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 483 --RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVgMQHDIKGQ-GI 559
Cdd:cd17650 318 aeRFVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VREDKGGEaRL 396
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 560 YVYVTlnsgvePSEALR-QELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17650 397 CAYVV------AAATLNtAELRAFLAKELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
108-616 |
1.49e-28 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 120.25 E-value: 1.49e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAiHSVVFG-GFSPEALAGRIIDCKSKVVITA 186
Cdd:PRK13382 69 LTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLNtSFAGPALAEVVTREGVDTVIYD 147
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEgvrggkrtpLKANVDDALTN-PETSSVqkiivckrtgaeIKWNQHRDVWYDDLMKVAGSTCAPKEMGAEDPLfILYTS 265
Cdd:PRK13382 148 EE---------FSATVDRALADcPQATRI------------VAWTDEDHDLTVEVLIAAHAGQRPEPTGRKGRV-ILLTS 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLHT-TGGYLVYASltherVFDYRPgevywctadigWVTGHTYIVYGPL--ANGATTILFEGVPNYPDVTR- 341
Cdd:PRK13382 206 GTTGTPKGARRSgPGGIGTLKA-----ILDRTP-----------WRAEEPTVIVAPMfhAWGFSQLVLAASLACTIVTRr 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 342 ------VAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDTWWQ 415
Cdd:PRK13382 270 rfdpeaTLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQFGDV---IYNNYNA 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 416 TETGAcLMTPLPGAHAMKPGSAAKPFFGVVPALVDnlgnliegaAEGNlvilDSWPGQARTLFGDHDRFVDTYF----KT 491
Cdd:PRK13382 347 TEAGM-IATATPADLRAAPDTAGRPAEGTEIRILD---------QDFR----EVPTGEVGTIFVRNDTQFDGYTsgstKD 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 492 F-KGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGve 570
Cdd:PRK13382 413 FhDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQRLAAFVVLKPG-- 490
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 15596084 571 pSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK13382 491 -ASATPETLKQHVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
531-609 |
1.72e-28 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 108.40 E-value: 1.72e-28
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15596084 531 EVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPseaLRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGK 609
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGVEL---LEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
87-615 |
2.11e-28 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 119.35 E-value: 2.11e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 87 AERG-DQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVfgg 165
Cdd:cd17655 7 AEKTpDHTAVVFED------QTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPI--- 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 166 fSPEALAGRII----DCKSKVVITADegvrggkrtPLKANVDDA----LTNPETSSvqkiivckrtgAEIKWNQHRDVWY 237
Cdd:cd17655 78 -DPDYPEERIQyileDSGADILLTQS---------HLQPPIAFIglidLLDEDTIY-----------HEESENLEPVSKS 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 238 DDLMkvagstcapkemgaedplFILYTSGSTGKPKGVLhttggylvyasLTHERVFDYrpgeVYWCTaDIGWVTGHTYI- 316
Cdd:cd17655 137 DDLA------------------YVIYTSGSTGKPKGVM-----------IEHRGVVNL----VEWAN-KVIYQGEHLRVa 182
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 -------------VYGPLANGATTILfegvpnYPDVTR-----VAKIIDKHKVNILYTAPTAIRAMMAEGKAavagaDGS 378
Cdd:cd17655 183 lfasisfdasvteIFASLLSGNTLYI------VRKETVldgqaLTQYIRQNRITIIDLTPAHLKLLDAADDS-----EGL 251
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 379 SLRLLGSVGEPINPEAWQWYYETVGQSrCPIVDTWWQTETGACLMTPLPGAHAMKPGSA--AKPFFGVVPALVDNLGNLI 456
Cdd:cd17655 252 SLKHLIVGGEALSTELAKKIIELFGTN-PTITNAYGPTETTVDASIYQYEPETDQQVSVpiGKPLGNTRIYILDQYGRPQ 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 457 EGAAEGNLVIldSWPGQARTLFGDHD----RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEV 532
Cdd:cd17655 331 PVGVAGELYI--GGEGVARGYLNRPEltaeKFVDDPFVPGERMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIELGEI 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 533 ESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSealrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17655 409 EARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELPVA-----QLREFLARELPDYMIPSYFIKLDEIPLTPNGKVDR 483
|
...
gi 15596084 613 RIL 615
Cdd:cd17655 484 KAL 486
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
107-617 |
2.30e-27 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 115.14 E-value: 2.30e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 107 EITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsVVFggfspeaLAGRIidckskvvita 186
Cdd:cd05912 1 SYTFAELFEEVSRLAEHLAALGVRKGDRVALLSKNSIEMILLIHALWLLGAE--AVL-------LNTRL----------- 60
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 degvrggkrtplkanvddaltnpetssvqkiivckrTGAEIKWN-QHRDVWYDDLMKvagstcapkemgaedplfILYTS 265
Cdd:cd05912 61 ------------------------------------TPNELAFQlKDSDVKLDDIAT------------------IMYTS 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLHTTGGYLVYASLTHERVFDYRpgEVYW-CTADIGWVTGHTYIVYGpLANGATTILFEGVpnypDVTRVAK 344
Cdd:cd05912 87 GTTGKPKGVQQTFGNHWWSAIGSALNLGLTE--DDNWlCALPLFHISGLSILMRS-VIYGMTVYLVDKF----DAEQVLH 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 345 IIDKHKVNILYTAPTAIRAMMAEGKAAVAgadgSSLR--LLGsvGEPINPEAwqwyYETVGQSRCPIVDTWWQTETGACL 422
Cdd:cd05912 160 LINSGKVTIISVVPTMLQRLLEILGEGYP----NNLRciLLG--GGPAPKPL----LEQCKEKGIPVYQSYGMTETCSQI 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 423 MTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEgaaEGNLVIldSWPGQARTLFGDHDR----FVDTYFKTfkgmyft 498
Cdd:cd05912 230 VTLSPEDALNKIGSAGKPLFPVELKIEDDGQPPYE---VGEILL--KGPNVTKGYLNRPDAteesFENGWFKT------- 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 499 GDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVepSEAlrqE 578
Cdd:cd05912 298 GDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGVVGIPDDKWGQVPVAFVVSERPI--SEE---E 372
|
490 500 510
....*....|....*....|....*....|....*....
gi 15596084 579 LKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05912 373 LIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
77-617 |
3.22e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 118.34 E-value: 3.22e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 77 VSHNCLDRHLAERGdqVAIIWEG------DDPA---DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVV 147
Cdd:PRK12467 500 VRWNAPATEYAPDC--VHQLIEAqarqhpERPAlvfGEQVLSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVV 577
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 148 AMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVRggkRTPLKANVDDALTNpetssvqkiivckrtgAEI 227
Cdd:PRK12467 578 GLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQSHLLA---QLPVPAGLRSLCLD----------------EPA 638
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 228 KWNQHRDVWYDDLMKVAGSTCapkemgaedplFILYTSGSTGKPKGVLHTTGGYLVYASLTHERvfdyrpgevYWCTADI 307
Cdd:PRK12467 639 DLLCGYSGHNPEVALDPDNLA-----------YVIYTSGSTGQPKGVAISHGALANYVCVIAER---------LQLAADD 698
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 308 GWVTGHTY-------IVYGPLANGATTILFEgvpnyPDVTR----VAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAgad 376
Cdd:PRK12467 699 SMLMVSTFafdlgvtELFGALASGATLHLLP-----PDCARdaeaFAALMADQGVTVLKIVPSHLQALLQASRVALP--- 770
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 377 gSSLRLLGSVGEPINPEAWQWYYETVGQSRcpIVDTWWQTETGACLMT-PLPGAHAMKPGSA-AKPFFGVVPALVDNLGN 454
Cdd:PRK12467 771 -RPQRALVCGGEALQVDLLARVRALGPGAR--LINHYGPTETTVGVSTyELSDEERDFGNVPiGQPLANLGLYILDHYLN 847
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 455 LIEGAAEGNLVIldSWPGQARTLFG----DHDRFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGT 529
Cdd:PRK12467 848 PVPVGVVGELYI--GGAGLARGYHRrpalTAERFVPDPFGADGGrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIEL 925
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 530 AEVESAMVAHPKVAEAAVVGmQHDIKGQGIYVYVTLNSGVEPSE--ALRQELKQWVRREIGPIATPDVIQWAPGLPKTRS 607
Cdd:PRK12467 926 GEIEARLLAQPGVREAVVLA-QPGDAGLQLVAYLVPAAVADGAEhqATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPN 1004
|
570
....*....|
gi 15596084 608 GKIMRRILRK 617
Cdd:PRK12467 1005 GKLDRKALPK 1014
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
91-615 |
3.22e-27 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 115.65 E-value: 3.22e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEgddpadHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGaihsvvfGGFSP-- 168
Cdd:cd17656 3 DAVAVVFE------NQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAG-------GAFVPid 69
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 169 -----EALAGRIIDCKSKVVITADEgvrggkrtpLKANVDDaltNPETSSVQKIIVCKRTGAEIKWnqhrdvwyddlmkv 243
Cdd:cd17656 70 peypeERRIYIMLDSGVRVVLTQRH---------LKSKLSF---NKSTILLEDPSISQEDTSNIDY-------------- 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 244 agstcapkEMGAEDPLFILYTSGSTGKPKGVL--HTTGGYLVYASLTH------ERVFDYRPGEVYWCTADI--GWVTGH 313
Cdd:cd17656 124 --------INNSDDLLYIIYTSGTTGKPKGVQleHKNMVNLLHFEREKtninfsDKVLQFATCSFDVCYQEIfsTLLSGG 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 314 T-YIVygplaNGATTIlfegvpnypDVTRVAKIIDKHKVNILYTaPTAIRAMMAEGKAAVAG-ADGssLRLLGSVGEPIn 391
Cdd:cd17656 196 TlYII-----REETKR---------DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREFINRfPTC--VKHIITAGEQL- 257
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 392 pEAWQWYYETVGQSRCPIVDTWWQTET---GACLMTPLPGAHAMKPgsAAKPFFGVVPALVDNLGNLIEGAAEGNLVIld 468
Cdd:cd17656 258 -VITNEFKEMLHEHNVHLHNHYGPSEThvvTTYTINPEAEIPELPP--IGKPISNTWIYILDQEQQLQPQGIVGELYI-- 332
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 469 SWPGQARTLFGD----HDRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAE 544
Cdd:cd17656 333 SGASVARGYLNRqeltAEKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSE 412
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15596084 545 AAVVGMQHDIKGQGIYVYVTLNSGVEPSealrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17656 413 AVVLDKADDKGEKYLCAYFVMEQELNIS-----QLREYLAKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
106-615 |
6.55e-27 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 114.68 E-value: 6.55e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHsVVFGGFSPEALAGRII-DCKSKVVI 184
Cdd:cd12114 11 GTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAY-VPVDIDQPAARREAILaDAGARLVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 185 TADEgvrggkrtPLKANVDDALTNPETSSVQkiivckrtgaeikwnqhrdvwyddlmkVAGSTCAPKEMGAEDPLFILYT 264
Cdd:cd12114 90 TDGP--------DAQLDVAVFDVLILDLDAL---------------------------AAPAPPPPVDVAPDDLAYVIFT 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 265 SGSTGKPKGVLHTTGGYL--------VYASLTHERV-------FDYrpgEVYwctaDIgwvtghtyivYGPLANGATTIL 329
Cdd:cd12114 135 SGSTGTPKGVMISHRAALntildinrRFAVGPDDRVlalsslsFDL---SVY----DI----------FGALSAGATLVL 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 330 fegvPNY---PDVTRVAKIIDKHKVNILYTAPtAIRAMMAEgKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSR 406
Cdd:cd12114 198 ----PDEarrRDPAHWAELIERHGVTLWNSVP-ALLEMLLD-VLEAAQALLPSLRLVLLSGDWIPLDLPARLRALAPDAR 271
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 407 cpIVDTWWQTETG----ACLMTPLPGAHAMKP-GsaaKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGD- 480
Cdd:cd12114 272 --LISLGGATEASiwsiYHPIDEVPPDWRSIPyG---RPLANQRYRVLDPRGRDCPDWVPGELWI--GGRGVALGYLGDp 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 481 ---HDRFVDTyfKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQhDIKGQ 557
Cdd:cd12114 345 eltAARFVTH--PDGERLYRTGDLGRYRPDGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVLG-DPGGK 421
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 558 GIYVYVTLNSGVEP--SEALRQELKQWVRReigpIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd12114 422 RLAAFVVPDNDGTPiaPDALRAFLAQTLPA----YMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
79-620 |
7.46e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 117.18 E-value: 7.46e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:PRK12467 3098 HQLIEAQVARTPEAPALVFGD------QQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGA 3171
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITadegvrggkrtplKANVDDALtnPETSSVQKIIVCKrtgaeikwnqhrdvwyD 238
Cdd:PRK12467 3172 YVPLDPEYPRERLAYMIEDSGVKLLLT-------------QAHLLEQL--PAPAGDTALTLDR----------------L 3220
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 DLMKVAGSTCAPKEMGaEDPLFILYTSGSTGKPKGV--------LHTTGGYLVYASLTHERV-------FDYRPGEVYWc 303
Cdd:PRK12467 3221 DLNGYSENNPSTRVMG-ENLAYVIYTSGSTGKPKGVgvrhgalaNHLCWIAEAYELDANDRVllfmsfsFDGAQERFLW- 3298
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 304 tadigwvtghtyivygPLANGATTILFEGVPNYPDVTRVAkiIDKHKVNILYTAPTAIRAMMAEGKaavaGADGSSLRLL 383
Cdd:PRK12467 3299 ----------------TLICGGCLVVRDNDLWDPEELWQA--IHAHRISIACFPPAYLQQFAEDAG----GADCASLDIY 3356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPINPEAWQWY---------YETVGQSRCPIVDTWWQT-ETGACLMTPLPGAHamkpgsaakPFFGVVPALVDNLG 453
Cdd:PRK12467 3357 VFGGEAVPPAAFEQVkrklkprglTNGYGPTEAVVTVTLWKCgGDAVCEAPYAPIGR---------PVAGRSIYVLDGQL 3427
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 454 NLIEGAAEGNLVIldSWPGQARtlfGDH-------DRFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGH 525
Cdd:PRK12467 3428 NPVPVGVAGELYI--GGVGLAR---GYHqrpsltaERFVADPFSGSGGrLYRTGDLARYRADGVIEYLGRIDHQVKIRGF 3502
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 526 RMGTAEVESAMVAHPKVAEAAVVGmQHDIKGQGIYVYVTLNSgvePSEALRQELKQWVRREIGPIATPDVIQWAPGLPKT 605
Cdd:PRK12467 3503 RIELGEIEARLLQHPSVREAVVLA-RDGAGGKQLVAYVVPAD---PQGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLG 3578
|
570
....*....|....*
gi 15596084 606 RSGKIMRRILRKIAA 620
Cdd:PRK12467 3579 PNGKVDRKALPDPDA 3593
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
107-615 |
8.32e-27 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 115.11 E-value: 8.32e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 107 EITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITA 186
Cdd:PRK05857 41 ALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQITDPAAALVA 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEGVRGGKRTPLKANVDDALTNPETSSVqkiivckrtgAEIKWNQHRDvwyddlmkvagSTCAPKEMGAEDPLFILYTSG 266
Cdd:PRK05857 121 PGSKMASSAVPEALHSIPVIAVDIAAVT----------RESEHSLDAA-----------SLAGNADQGSEDPLAMIFTSG 179
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 267 STGKPKGVLHTTGGYLVYASLTHERVFDYrpgevywctadIGWVTGHTyiVYGPLAngATTI---------LFEG---VP 334
Cdd:PRK05857 180 TTGEPKAVLLANRTFFAVPDILQKEGLNW-----------VTWVVGET--TYSPLP--ATHIgglwwiltcLMHGglcVT 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 335 NYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKaaVAGADGSSLRLLGSVGEPInPEAWQWYYETVGQSRCPIvdtWW 414
Cdd:PRK05857 245 GGENTTSLLEILTTNAVATTCLVPTLLSKLVSELK--SANATVPSLRLVGYGGSRA-IAADVRFIEATGVRTAQV---YG 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 QTETGA---CLMTPLPGAHAMKPGSAAKPFFGVVPALVD------NLGNLIEGAAEGNLVI------LDSWPGQARTlfg 479
Cdd:PRK05857 319 LSETGCtalCLPTDDGSIVKIEAGAVGRPYPGVDVYLAAtdgigpTAPGAGPSASFGTLWIkspanmLGYWNNPERT--- 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 480 dHDRFVDtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQ-- 557
Cdd:PRK05857 396 -AEVLID-------GWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAACYEIPDEEFGAlv 467
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 558 GIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK05857 468 GLAVVASAELDESAARALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASL 525
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
257-619 |
1.11e-26 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 111.27 E-value: 1.11e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 257 DPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVfDYRPGEVYWCTADIGWVTGhTYIVYGPLANGATTILFEGVPny 336
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAANLLASAAGLHSRL-GFGGGDSWLLSLPLYHVGG-LAILVRSLLAGAELVLLERNQ-- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 337 pdvtrvAKIIDKHKVNILYTA--PTAIRAMMAEGKAAvagADGSSLRLLGSVGEPINPEAwqwyYETVGQSRCPIVDTWW 414
Cdd:cd17630 77 ------ALAEDLAPPGVTHVSlvPTQLQRLLDSGQGP---AALKSLRAVLLGGAPIPPEL----LERAADRGIPLYTTYG 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 QTETGACLMTPLPGAHamKPGSAAKPFFGVVPALVDNlgNLIEGAAEGnlVILDSWPGQARTLFGDhdrfvdtyfktfKG 494
Cdd:cd17630 144 MTETASQVATKRPDGF--GRGGVGVLLPGRELRIVED--GEIWVGGAS--LAMGYLRGQLVPEFNE------------DG 205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 495 MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSea 574
Cdd:cd17630 206 WFTTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADPA-- 283
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 15596084 575 lrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:cd17630 284 ---ELRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
106-625 |
4.08e-26 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 112.29 E-value: 4.08e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:PRK06145 26 QEISYAEFHQRILQAAGMLHARGIGQGDVVALLMKNSAAFLELAFAASYLGAVFLPINYRLAADEVAYILGDAGAKLLLV 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 ADEgvrggkrtpLKANVddaltnpetSSVQKIIVckrTGAEIKWNQHRdvwyddlMKVAGSTCAPKEMGAEDPLF-ILYT 264
Cdd:PRK06145 106 DEE---------FDAIV---------ALETPKIV---IDAAAQADSRR-------LAQGGLEIPPQAAVAPTDLVrLMYT 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 265 SGSTGKPKGVLHTTGgylvyaslthervfdyrpgEVYWCTAD----IGWVTGHTYIVYGPLAN-GA-----TTILFEG-- 332
Cdd:PRK06145 158 SGTTDRPKGVMHSYG-------------------NLHWKSIDhviaLGLTASERLLVVGPLYHvGAfdlpgIAVLWVGgt 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 333 --VPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRcpIV 410
Cdd:PRK06145 219 lrIHREFDPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRD--RFDLDSLAWCIGGGEKTPESRIRDFTRVFTRAR--YI 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 411 DTWWQTET--GACLMTPlpGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDswPGQARTLFGDHDR----F 484
Cdd:PRK06145 295 DAYGLTETcsGDTLMEA--GREIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGEICMRG--PKVTKGYWKDPEKtaeaF 370
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 485 VDTYFKTfkgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVT 564
Cdd:PRK06145 371 YGDWFRS-------GDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVAEAAVIGVHDDRWGERITAVVV 443
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15596084 565 LNSGvepSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRkiaaAEYDT 625
Cdd:PRK06145 444 LNPG---ATLTLEALDRHCRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLR----DELNG 497
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
83-617 |
8.89e-26 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 110.48 E-value: 8.89e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 83 DRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVV 162
Cdd:cd17653 4 ERIAAAHPDAVAVESLG------GSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPL 77
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 163 FGGfSPEALAGRIID-CKSKVVITADegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlm 241
Cdd:cd17653 78 DAK-LPSARIQAILRtSGATLLLTTD------------------------------------------------------ 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 242 kvagstcapkemGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERvFDYRPGE--------VYWCTAdigWVtgh 313
Cdd:cd17653 103 ------------SPDDLAYIIFTSGSTGIPKGVMVPHRGVLNYVSQPPAR-LDVGPGSrvaqvlsiAFDACI---GE--- 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 314 tyiVYGPLANGATTILFEGVPNYPDVTRvakiidkhKVNILYTAPTAIrammaegkAAVAGADGSSLRLLGSVGEPINPe 393
Cdd:cd17653 164 ---IFSTLCNGGTLVLADPSDPFAHVAR--------TVDALMSTPSIL--------STLSPQDFPNLKTIFLGGEAVPP- 223
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 394 awqwyyetvgqsrcPIVDTWWQ----------TETG-ACLMTPL-PGahamKPGSAAKPFFGVVPALVDNLGNLIEGAAE 461
Cdd:cd17653 224 --------------SLLDRWSPgrrlynaygpTECTiSSTMTELlPG----QPVTIGKPIPNSTCYILDADLQPVPEGVV 285
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 462 GNLVIldSWPGQARTLFGDH----DRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMV 537
Cdd:cd17653 286 GEICI--SGVQVARGYLGNPaltaSKFVPDPFWPGSRMYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVL 363
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 538 AHPKVAEAAVVgMQHDikgQGIYVYVTLNSGVEpsEALRQELkqwvRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd17653 364 QSQPEVTQAAA-IVVN---GRLVAFVTPETVDV--DGLRSEL----AKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
105-622 |
9.34e-26 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 111.79 E-value: 9.34e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 105 HQEI--TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKV 182
Cdd:PRK12583 41 HQALryTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRW 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 183 VITADE-------GVRGGKRTPLKANVDDALTNPETSSVQKIIVCKRTGAEikwnqHRDVWYDdlMKVAGSTCAPKEMGA 255
Cdd:PRK12583 121 VICADAfktsdyhAMLQELLPGLAEGQPGALACERLPELRGVVSLAPAPPP-----GFLAWHE--LQARGETVSREALAE 193
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 -------EDPLFILYTSGSTGKPKGVLHT-----TGGYLVYASL---THERVFdyRPGEVYWCTadigwvtGHTYIVYGP 320
Cdd:PRK12583 194 rqasldrDDPINIQYTSGTTGFPKGATLShhnilNNGYFVAESLgltEHDRLC--VPVPLYHCF-------GMVLANLGC 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 321 LANGATTILfegvPN-YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKaaVAGADGSSLRLLGSVGEPINPEAWQwyy 399
Cdd:PRK12583 265 MTVGACLVY----PNeAFDPLATLQAVEEERCTALYGVPTMFIAELDHPQ--RGNFDLSSLRTGIMAGAPCPIEVMR--- 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 400 ETVGQSRCP-IVDTWWQTETG-ACLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNL------VILDSWP 471
Cdd:PRK12583 336 RVMDEMHMAeVQIAYGMTETSpVSLQTTAADDLERRVETVGRTQPHLEVKVVDPDGATVPRGEIGELctrgysVMKGYWN 415
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 472 GQARTLFG-DHDrfvdtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGM 550
Cdd:PRK12583 416 NPEATAESiDED-----------GWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGV 484
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 551 QHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK12583 485 PDEKYGEEIVAWVRLHPGHAASE---EELREFCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREISIEE 553
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
79-621 |
1.02e-25 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 113.90 E-value: 1.02e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:PRK12316 3060 HRLFEEQVERTPDAVALAFGE------QRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGA 3133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITADEgvrggkrtplkanvddaLTNPETSSVQKIIVckrtgaeikwnqhrdvwyD 238
Cdd:PRK12316 3134 YVPLDPEYPEERLAYMLEDSGAQLLLSQSH-----------------LRLPLAQGVQVLDL------------------D 3178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 DLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVL--------HTTGGYLVYASLTHERVFDYRPGEVywctadigwv 310
Cdd:PRK12316 3179 RGDENYAEANPAIRTMPENLAYVIYTSGSTGKPKGVGirhsalsnHLCWMQQAYGLGVGDRVLQFTTFSF---------- 3248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 311 TGHTYIVYGPLANGATTILfEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvagaDGSSLRLLGSVGEPI 390
Cdd:PRK12316 3249 DVFVEELFWPLMSGARVVL-AGPEDWRDPALLVELINSEGVDVLHAYPSMLQAFLEEEDAH----RCTSLKRIVCGGEAL 3323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 391 NPEAWQWYYetvgqSRCPIVDTWWQTETgaclmTPLPGAHAMKPGSAAKPFFGVVPA-----LVDNLGNLIEGAAEGNLV 465
Cdd:PRK12316 3324 PADLQQQVF-----AGLPLYNLYGPTEA-----TITVTHWQCVEEGKDAVPIGRPIAnracyILDGSLEPVPVGALGELY 3393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 466 IldSWPGQARTLFGDHD----RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPK 541
Cdd:PRK12316 3394 L--GGEGLARGYHNRPGltaeRFVPDPFVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPW 3471
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 542 VAEAAVVgmqhDIKGQGIYVYVTLNSgvePSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:PRK12316 3472 VREAVVL----AVDGRQLVAYVVPED---EAGDLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAA 3544
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
79-622 |
1.32e-25 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 113.51 E-value: 1.32e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:PRK12316 2006 HQRIAEQAARAPEAIAVV------FGDQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGA 2079
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITaDEGVRggKRTPLKANVDDALTNPEtssvqkiivckrtgaeikwnqhrDVWYD 238
Cdd:PRK12316 2080 YVPLDPNYPAERLAYMLEDSGAALLLT-QRHLL--ERLPLPAGVARLPLDRD-----------------------AEWAD 2133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 dlmkvaGSTCAPKEMGAEDPL-FILYTSGSTGKPKGVLHTTGGYLVYASLTHERvFDYRPGEVYWCTADIGWVTGHTYIv 317
Cdd:PRK12316 2134 ------YPDTAPAVQLAGENLaYVIYTSGSTGLPKGVAVSHGALVAHCQAAGER-YELSPADCELQFMSFSFDGAHEQW- 2205
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 YGPLANGATTILFEGVPNYPDvtRVAKIIDKHKVNILYTAPTAIRAMmaegkAAVAGADGS--SLRLLGSVGEPINPEAW 395
Cdd:PRK12316 2206 FHPLLNGARVLIRDDELWDPE--QLYDEMERHGVTILDFPPVYLQQL-----AEHAERDGRppAVRVYCFGGEAVPAASL 2278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 396 QWYYETVGQSRcpIVDTWWQTETgacLMTPLpgAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAA--------EGNLVIl 467
Cdd:PRK12316 2279 RLAWEALRPVY--LFNGYGPTEA---VVTPL--LWKCRPQDPCGAAYVPIGRALGNRRAYILDADlnllapgmAGELYL- 2350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 dSWPGQARTLFG----DHDRFV-DTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKV 542
Cdd:PRK12316 2351 -GGEGLARGYLNrpglTAERFVpDPFSASGERLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAV 2429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 543 AEAAVVGmQHDIKGQGIYVYVTlnsGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK12316 2430 REAVVVA-QDGASGKQLVAYVV---PDDAAEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQ 2505
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
125-616 |
1.38e-25 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 110.54 E-value: 1.38e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 125 RGQDVHRGDVVTIYMPM--IPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADEGVRGGKRTPLKAnv 202
Cdd:cd05929 13 HQRRLLLLDVYSIALNRnaRAAAAEGVWIADGVYIYLINSILTVFAAAAAWKCGACPAYKSSRAPRAEACAIIEIKAA-- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 203 ddaltnpetssvqkIIVCKRTGAeikWNQHrDVWYDDLMKVAGSTCAPKEMGAEdPLFILYTSGSTGKPKGVLHTTGGYL 282
Cdd:cd05929 91 --------------ALVCGLFTG---GGAL-DGLEDYEAAEGGSPETPIEDEAA-GWKMLYSGGTTGRPKGIKRGLPGGP 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 283 VYASLTHERVFDYRPGE--VYWCTADIGWVTGHTYiVYGPLANGATTILFEGVpnypDVTRVAKIIDKHKVNILYTAPTA 360
Cdd:cd05929 152 PDNDTLMAAALGFGPGAdsVYLSPAPLYHAAPFRW-SMTALFMGGTLVLMEKF----DPEEFLRLIERYRVTFAQFVPTM 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 361 IRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEA------------WQWYYETVGQSRCPIVDTWWQTetgaclmtplpg 428
Cdd:cd05929 227 FVRLLKLPEAVRNAYDLSSLKRVIHAAAPCPPWVkeqwidwggpiiWEYYGGTEGQGLTIINGEEWLT------------ 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 429 aHamkPGSAAKPFFGVVpALVDNLGNLIEGAAEGNLVILDSWPgqartlFGDHDRFVDTYFKTFKGMYFT-GDGARRDED 507
Cdd:cd05929 295 -H---PGSVGRAVLGKV-HILDEDGNEVPPGEIGEVYFANGPG------FEYTNDPEKTAAARNEGGWSTlGDVGYLDED 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 508 GYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREI 587
Cdd:cd05929 364 GYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVVQPAPGADAGTALAEELIAFLRDRL 443
|
490 500
....*....|....*....|....*....
gi 15596084 588 GPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05929 444 SRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
256-612 |
1.40e-25 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 108.50 E-value: 1.40e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 EDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEgvpn 335
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVTGGE---- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 336 YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADgsSLRLLGSVGE-PINPEAWqwYYETVGQSRcpIVDTWW 414
Cdd:cd17635 77 NTTYKSLFKILTTNAVTTTCLVPTLLSKLVSELKSANATVP--SLRLIGYGGSrAIAADVR--FIEATGLTN--TAQVYG 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 QTETGACLMTPLpGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSW--PGQARTLFGDHDRFVDTYFKTf 492
Cdd:cd17635 151 LSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPAnmLGYWNNPERTAEVLIDGWVNT- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 493 kgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLnSGVEPS 572
Cdd:cd17635 229 ------GDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVA-SAELDE 301
|
330 340 350 360
....*....|....*....|....*....|....*....|
gi 15596084 573 EALRQeLKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17635 302 NAIRA-LKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
109-617 |
2.31e-25 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 110.61 E-value: 2.31e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVI--TA 186
Cdd:PRK06087 51 TYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFapTL 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEGVRGGKR-TPLKANVD--------DALTnPETSSVQKIIVCKRtgaeikwnqhrdvwYDDLMkvagstcAPKEMGAED 257
Cdd:PRK06087 131 FKQTRPVDLiLPLQNQLPqlqqivgvDKLA-PATSSLSLSQIIAD--------------YEPLT-------TAITTHGDE 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 258 PLFILYTSGSTGKPKGVLhttggylvyasLTH------ERVFDYRPG----EVYWCTADIGWVTGHTYIVYGPLANGATT 327
Cdd:PRK06087 189 LAAVLFTSGTEGLPKGVM-----------LTHnnilasERAYCARLNltwqDVFMMPAPLGHATGFLHGVTAPFLIGARS 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 328 ILFEGVPnyPDvtRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLGSVGEPInP-----EAWQWyyetv 402
Cdd:PRK06087 258 VLLDIFT--PD--ACLALLEQQRCTCMLGATPFIYDLLNLLEKQ--PADLSALRFFLCGGTTI-PkkvarECQQR----- 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 403 GQSRCPIvdtWWQTETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVDN------LGNLIEGAAEGNLVI---LDSWPGQ 473
Cdd:PRK06087 326 GIKLLSV---YGSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEarktlpPGCEGEEASRGPNVFmgyLDEPELT 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 474 ARTLfgDHDrfvdtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHD 553
Cdd:PRK06087 403 ARAL--DEE-----------GWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDE 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15596084 554 IKGQGIYVYVTLnSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK06087 470 RLGERSCAYVVL-KAPHHSLTLEEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRK 532
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
79-621 |
2.56e-25 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 112.36 E-value: 2.56e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:PRK12316 4554 HQLVAERARMTPDAVAVVFDE------EKLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGA 4627
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITADegvRGGKRTPLKANVDDaltnpetssvqkiIVCKRTGaeiKWNQHRDvwYD 238
Cdd:PRK12316 4628 YVPLDPEYPRERLAYMMEDSGAALLLTQS---HLLQRLPIPDGLAS-------------LALDRDE---DWEGFPA--HD 4686
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 DLMKVAGSTCApkemgaedplFILYTSGSTGKPKGVLHTTGGYLVYASLTHERvfdyrpgevYWCTADIGWVTGHTYI-- 316
Cdd:PRK12316 4687 PAVRLHPDNLA----------YVIYTSGSTGRPKGVAVSHGSLVNHLHATGER---------YELTPDDRVLQFMSFSfd 4747
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 -----VYGPLANGATTILFEgvPNYPDVTRVAKIIDKHKVNILYTAPTAIRaMMAEGkaAVAGADGSSLRLLGSVGEPIN 391
Cdd:PRK12316 4748 gshegLYHPLINGASVVIRD--DSLWDPERLYAEIHEHRVTVLVFPPVYLQ-QLAEH--AERDGEPPSLRVYCFGGEAVA 4822
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 392 PEAWQ---------WYYETVGQSRCPIVDTWWqtetgACLMTPLPGAHAMKPGsaaKPFFGVVPALVDNLGNLIEGAAEG 462
Cdd:PRK12316 4823 QASYDlawralkpvYLFNGYGPTETTVTVLLW-----KARDGDACGAAYMPIG---TPLGNRSGYVLDGQLNPLPVGVAG 4894
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 463 NLVIldSWPGQARTLFGDHD----RFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMV 537
Cdd:PRK12316 4895 ELYL--GGEGVARGYLERPAltaeRFVPDPFGAPGGrLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLR 4972
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 538 AHPKVAEAAVVGmQHDIKGQGIYVYVT-----LNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:PRK12316 4973 EHPAVREAVVIA-QEGAVGKQLVGYVVpqdpaLADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDR 5051
|
....*....
gi 15596084 613 RILRKIAAA 621
Cdd:PRK12316 5052 KALPQPDAS 5060
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
24-81 |
3.70e-25 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 98.31 E-value: 3.70e-25
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 24 YKTMYQQSVVNPDGFWREQAQRIDWIKPFEKVKQTSFDDHhvdIKWFADGTLNVSHNC 81
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFKPFDKVLDGSNGPF---AKWFVGGKLNVCYNC 55
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
91-615 |
4.07e-25 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 108.49 E-value: 4.07e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEGDdpadhqEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEA 170
Cdd:cd17652 2 DAPAVVFGDE------TLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 171 LAGRIIDCKSKVVITAdegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcap 250
Cdd:cd17652 76 IAYMLADARPALLLTT---------------------------------------------------------------- 91
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 251 kemgAEDPLFILYTSGSTGKPKGVL--HTTGGYLVYASLTHervFDYRPGEVYWCTADIGWvTGHTYIVYGPLANGATTI 328
Cdd:cd17652 92 ----PDNLAYVIYTSGSTGRPKGVVvtHRGLANLAAAQIAA---FDVGPGSRVLQFASPSF-DASVWELLMALLAGATLV 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 329 LfegVPNYPDVT--RVAKIIDKHKVNILYTAPTAIRAMMAegkaavagADGSSLRLLGSVGEPINPE-AWQWyyetvGQS 405
Cdd:cd17652 164 L---APAEELLPgePLADLLREHRITHVTLPPAALAALPP--------DDLPDLRTLVVAGEACPAElVDRW-----APG 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 406 RCpIVDTWWQTETGAC--LMTPLPGAhamkpgsaakpffGVVPalvdnLGNLIEGAAegnLVILDSW------------- 470
Cdd:cd17652 228 RR-MINAYGPTETTVCatMAGPLPGG-------------GVPP-----IGRPVPGTR---VYVLDARlrpvppgvpgely 285
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 471 ---PGQARtlfGDHD-------RFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAH 539
Cdd:cd17652 286 iagAGLAR---GYLNrpgltaeRFVADPFGAPGSrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEH 362
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15596084 540 PKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17652 363 PGVAEAVVVVRDDRPGDKRLVAYVVPAPGAAPTAA---ELRAHLAERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
90-616 |
6.13e-25 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 109.00 E-value: 6.13e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 90 GDQVAIIWEgDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPE 169
Cdd:PRK08008 21 GHKTALIFE-SSGGVVRRYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLRE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 170 ALAGRIIDCKSKVVITADE------GVRGGKRTPLKANVDDALTNPETSSVqkiivckrtgaeIKWNQHRDvwyddlmKV 243
Cdd:PRK08008 100 ESAWILQNSQASLLVTSAQfypmyrQIQQEDATPLRHICLTRVALPADDGV------------SSFTQLKA-------QQ 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 244 AGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTT-----GGYlvYAS-----------LTHERVF--DYRpgevywCTA 305
Cdd:PRK08008 161 PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHynlrfAGY--YSAwqcalrdddvyLTVMPAFhiDCQ------CTA 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 306 DIGWVTGhtyivygplanGATTILFEgvpNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLR---- 381
Cdd:PRK08008 233 AMAAFSA-----------GATFVLLE---KY-SARAFWGQVCKYRATITECIPMMIRTLMVQ--PPSANDRQHCLRevmf 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 382 LLgsvgePINPEAWQWYYETVGQSrcpIVDTWWQTETGACLMTPLPGAHAMKPgSAAKPFFGVVPALVDNLGNLIEGAAE 461
Cdd:PRK08008 296 YL-----NLSDQEKDAFEERFGVR---LLTSYGMTETIVGIIGDRPGDKRRWP-SIGRPGFCYEAEIRDDHNRPLPAGEI 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 462 GNLVIlDSWPGqaRTLFGDHDRFVDTYFKTFK--GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAH 539
Cdd:PRK08008 367 GEICI-KGVPG--KTIFKEYYLDPKATAKVLEadGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATH 443
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 540 PKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK08008 444 PKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSE---EEFFAFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIKKNLK 517
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
113-616 |
1.81e-24 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 107.48 E-value: 1.81e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 113 LHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT-AD--EG 189
Cdd:PRK12406 17 LAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARVLIAhADllHG 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 190 VRGgkRTPLKANVDDALTNPETSSVQKIIVCKRT---GAeIKWnqhrDVWyddLMKVAGSTCAPKEmgaeDPLFILYTSG 266
Cdd:PRK12406 97 LAS--ALPAGVTVLSVPTPPEIAAAYRISPALLTppaGA-IDW----EGW---LAQQEPYDGPPVP----QPQSMIYTSG 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 267 STGKPKGVLHT--TGGYLVYASLTHERVFDYRPGEVYWCTadigwvtghtyivyGPL------ANGATTILFEGV----P 334
Cdd:PRK12406 163 TTGHPKGVRRAapTPEQAAAAEQMRALIYGLKPGIRALLT--------------GPLyhsapnAYGLRAGRLGGVlvlqP 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 335 NYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrcP-IVDTW 413
Cdd:PRK12406 229 RF-DPEELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAPCPADVKRAMIEWWG----PvIYEYY 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 414 WQTETGACLMTPLPGAHAmKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNlvILDSWPGQARTLFGDHD---RFVDTyfk 490
Cdd:PRK12406 304 GSTESGAVTFATSEDALS-HPGTVGKAAPGAELRFVDEDGRPLPQGEIGE--IYSRIAGNPDFTYHNKPekrAEIDR--- 377
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 491 tfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVE 570
Cdd:PRK12406 378 --GGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEALMAVVEPQPGAT 455
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 15596084 571 PSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK12406 456 LDEA---DIRAQLKARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
101-622 |
2.13e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 107.20 E-value: 2.13e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 101 DPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKS 180
Cdd:PRK09088 16 DLALGRRWTYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEP 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 181 KVvITADEGVRGGKRTP-----LKANVDdALTNPETSSVQkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgA 255
Cdd:PRK09088 96 RL-LLGDDAVAAGRTDVedlaaFIASAD-ALEPADTPSIP---------------------------------------P 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 EDPLFILYTSGSTGKPKGVL-------HTTGGYLVYASLTHERVFdyrpgevyWCTADIGWVTGHTYIVYGPLANGATTI 328
Cdd:PRK09088 135 ERVSLILFTSGTSGQPKGVMlsernlqQTAHNFGVLGRVDAHSSF--------LCDAPMFHIIGLITSVRPVLAVGGSIL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 329 LFEGVpnypDVTRVAKIIDKHKVNIlyTAPTAIRAMMAEGKAAvAGADGSSLRLLGSV---GEPINPEAWQWYYEtvgqS 405
Cdd:PRK09088 207 VSNGF----EPKRTLGRLGDPALGI--THYFCVPQMAQAFRAQ-PGFDAAALRHLTALftgGAPHAAEDILGWLD----D 275
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 406 RCPIVDTWWQTETGACLMTPL-PGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHDRF 484
Cdd:PRK09088 276 GIPMVDGFGMSEAGTVFGMSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLL--RGPNLSPGYWRRPQAT 353
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 485 VDTYfkTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVT 564
Cdd:PRK09088 354 ARAF--TGDGWFRTGDIARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIV 431
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 565 LNSGvepSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK09088 432 PADG---APLDLERIRSHLSTRLAKYKVPKHLRLVDALPRTASGKLQKARLRDALAAG 486
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
79-621 |
3.80e-24 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 106.09 E-value: 3.80e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAI-IWEGDdpadhqeITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGa 157
Cdd:cd05918 2 HDLIEERARSQPDAPAVcAWDGS-------LTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAG- 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 158 ihsvvfGGFSPealagriIDckskvvitadegvrggkrtplkanvddaLTNPEtSSVQKIivCKRTGAEIkwnqhrdvwy 237
Cdd:cd05918 74 ------GAFVP-------LD----------------------------PSHPL-QRLQEI--LQDTGAKV---------- 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 238 ddlmkVAGSTcapkemgAEDPLFILYTSGSTGKPKGVL--HTTggyLVYASLTHERVFDYRPGE-VYW--------CTAD 306
Cdd:cd05918 100 -----VLTSS-------PSDAAYVIFTSGSTGKPKGVVieHRA---LSTSALAHGRALGLTSESrVLQfasytfdvSILE 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 307 IgwvtghtyivYGPLANGATTIlfegVPnyPDVTRV---AKIIDKHKVNILYTAPTAIRAMMAEgkaavagaDGSSLRLL 383
Cdd:cd05918 165 I----------FTTLAAGGCLC----IP--SEEDRLndlAGFINRLRVTWAFLTPSVARLLDPE--------DVPSLRTL 220
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPINPEawqwyyetvgqsrcpIVDTWWQ----------TEtgACLMT----PLPGAHAMKPGSAakpfFGVVPALV 449
Cdd:cd05918 221 VLGGEALTQS---------------DVDTWADrvrlinaygpAE--CTIAAtvspVVPSTDPRNIGRP----LGATCWVV 279
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 450 D--NLGNLIEGAAEGNLVIldSWPGQARTLFGDHDR----FVDT-------YFKTFKGMYFTGDGARRDEDG--YYwiTG 514
Cdd:cd05918 280 DpdNHDRLVPIGAVGELLI--EGPILARGYLNDPEKtaaaFIEDpawlkqeGSGRGRRLYRTGDLVRYNPDGslEY--VG 355
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 515 RVDDVLNVSGHRMGTAEVES-------------AMVAHPKVAE-----AAVVGMQHDIKGQGIyvyvTLNSGVEPSEALR 576
Cdd:cd05918 356 RKDTQVKIRGQRVELGEIEHhlrqslpgakevvVEVVKPKDGSsspqlVAFVVLDGSSSGSGD----GDSLFLEPSDEFR 431
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 15596084 577 ---QELKQWVRREIGPIATPDViqWAP--GLPKTRSGKIMRRILRKIAAA 621
Cdd:cd05918 432 alvAELRSKLRQRLPSYMVPSV--FLPlsHLPLTASGKIDRRALRELAES 479
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
106-616 |
5.79e-24 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 105.86 E-value: 5.79e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPeALAGRII-DCKSKVVI 184
Cdd:PRK13390 23 EQVSYRQLDDDSAALARVLYDAGLRTGDVVALLSDNSPEALVVLWAALRSGLYITAINHHLTA-PEADYIVgDSGARVLV 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 185 T--ADEGV--RGGKRTPLKANVddaltnpetssvqkiivckrtGAEIKWNQHrdvwYDDLMKVAGSTCAPKEMGAedplF 260
Cdd:PRK13390 102 AsaALDGLaaKVGADLPLRLSF---------------------GGEIDGFGS----FEAALAGAGPRLTEQPCGA----V 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 261 ILYTSGSTGKPKGVLHTTGGYLVYAS-----LTHERVFDYRPGEVYWCTADIGwvtgHTyivyGPL-------ANGATTI 328
Cdd:PRK13390 153 MLYSSGTTGFPKGIQPDLPGRDVDAPgdpivAIARAFYDISESDIYYSSAPIY----HA----APLrwcsmvhALGGTVV 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 329 LFEGVpnypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrcP 408
Cdd:PRK13390 225 LAKRF----DAQATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPCPVDVKHAMIDWLG----P 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 409 IVDTWWQTeTGACLMTPLPGAHAMK-PGSAAKPFFGVVpALVDNLGNliegaaegnlvilDSWPGQARTLFGDHDRFVDT 487
Cdd:PRK13390 297 IVYEYYSS-TEAHGMTFIDSPDWLAhPGSVGRSVLGDL-HICDDDGN-------------ELPAGRIGTVYFERDRLPFR 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 488 YF----KTFKGMY-------FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKG 556
Cdd:PRK13390 362 YLndpeKTAAAQHpahpfwtTVGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMG 441
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 557 QGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK13390 442 EQVKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
107-617 |
1.05e-23 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 105.21 E-value: 1.05e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 107 EITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITA 186
Cdd:cd05915 24 RTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHAEDKVLLFD 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEGVRGGKRT-PLKANVDDaltNPETSSvqkiivckrtgaeiKWNQhrdvwYDDLMKVAGSTCAP-KEMGAEDPLFILYT 264
Cdd:cd05915 104 PNLLPLVEAIrGELKTVQH---FVVMDE--------------KAPE-----GYLAYEEALGEEADpVRVPERAACGMAYT 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 265 SGSTGKPKGVLHT-TGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIvYGPLANGATTILFEGVPNypDVTRVA 343
Cdd:cd05915 162 TGTTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLP-YAATLVGAKQVLPGPRLD--PASLVE 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 344 KIIdKHKVNILYTAPTAIRaMMAEGKAAVAGADGSSLRLLGSVGEPinPEAwQWYYETVGQSRcpIVDTWWQTET----G 419
Cdd:cd05915 239 LFD-GEGVTFTAGVPTVWL-ALADYLESTGHRLKTLRRLVVGGSAA--PRS-LIARFERMGVE--VRQGYGLTETspvvV 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 420 ACLMTP----LPGAHAM--KPGSAAKPFFGVVPAL------VDNLGNLIEGAA-EGNLVIldswpgqaRTLFGDHDRFVD 486
Cdd:cd05915 312 QNFVKShlesLSEEEKLtlKAKTGLPIPLVRLRVAdeegrpVPKDGKALGEVQlKGPWIT--------GGYYGNEEATRS 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 487 TYFKtfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLN 566
Cdd:cd05915 384 ALTP--DGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVVVPR 461
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 15596084 567 SgvepSEALRQELKQWVRREIGPIAT-PDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05915 462 G----EKPTPEELNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
81-621 |
1.09e-23 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 105.45 E-value: 1.09e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 81 CLDRhLAERGDQVAIIwegdDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHS 160
Cdd:PLN02246 29 CFER-LSEFSDRPCLI----DGATGRVYTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTT 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 161 VVFGGFSPEALAGRIIDCKSKVVITadEGVRGGKRTPLKAnvDDALTnpetssvqkiIVCKrtgaeikwNQHRD--VWYD 238
Cdd:PLN02246 104 TANPFYTPAEIAKQAKASGAKLIIT--QSCYVDKLKGLAE--DDGVT----------VVTI--------DDPPEgcLHFS 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 DLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLhttggylvyasLTHERV--------------FDYRPGEVYWCT 304
Cdd:PLN02246 162 ELTQADENELPEVEISPDDVVALPYSSGTTGLPKGVM-----------LTHKGLvtsvaqqvdgenpnLYFHSDDVILCV 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 305 ADIGwvtgHTY----IVYGPLANGATTILfegVPNYpDVTRVAKIIDKHKVNIlytAPTAIRAMMAEGKA-AVAGADGSS 379
Cdd:PLN02246 231 LPMF----HIYslnsVLLCGLRVGAAILI---MPKF-EIGALLELIQRHKVTI---APFVPPIVLAIAKSpVVEKYDLSS 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 380 LRLLGSVGEPINPEawqwyYETVGQSRCP---IVDTWWQTETGACLmtplpgahAMKPGSAAKPF---FGVVPALVDNlg 453
Cdd:PLN02246 300 IRMVLSGAAPLGKE-----LEDAFRAKLPnavLGQGYGMTEAGPVL--------AMCLAFAKEPFpvkSGSCGTVVRN-- 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 454 nliegaAEGNLVILDSWPGQARTLFGD----HDRFVDTYF-------KTF--KGMYFTGDGARRDEDGYYWITGRVDDVL 520
Cdd:PLN02246 365 ------AELKIVDPETGASLPRNQPGEicirGPQIMKGYLndpeataNTIdkDGWLHTGDIGYIDDDDELFIVDRLKELI 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 521 NVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRRE------IGPIATPD 594
Cdd:PLN02246 439 KYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPVAFVVRSNGSEITE---DEIKQFVAKQvvfykrIHKVFFVD 515
|
570 580
....*....|....*....|....*..
gi 15596084 595 VIqwapglPKTRSGKIMRRILRKIAAA 621
Cdd:PLN02246 516 SI------PKAPSGKILRKDLRAKLAA 536
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
107-619 |
4.25e-23 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 103.18 E-value: 4.25e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 107 EITYRQLHEQVCKFANALRgQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITA 186
Cdd:cd05909 7 SLTYRKLLTGAIALARKLA-KMTKEGENVGVMLPPSAGGALANFALALSGKVPVMLNYTAGLRELRACIKLAGIKTVLTS 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEGVRGGKRTPLKANVDDA-------LTNpETSSVQKIivckRTGAEIKWNQhrdvwyDDLMKVAGstCAPKEmgAEDPL 259
Cdd:cd05909 86 KQFIEKLKLHHLFDVEYDArivyledLRA-KISKADKC----KAFLAGKFPP------KWLLRIFG--VAPVQ--PDDPA 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 260 FILYTSGSTGKPKGVLHTTGGYL--VYASLTHervFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFegvPNYP 337
Cdd:cd05909 151 VILFTSGSEGLPKGVVLSHKNLLanVEQITAI---FDPNPEDVVFGALPFFHSFGLTGCLWLPLLSGIKVVFH---PNPL 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 338 DVTRVAKIIDKHKVNILYTAPTAIRAMMaegKAAVAgADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTE 417
Cdd:cd05909 225 DYKKIPELIYDKKATILLGTPTFLRGYA---RAAHP-EDFSSLRLVVAGAEKLKDTLRQEFQEKFG---IRILEGYGTTE 297
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 418 TGACLMTPLPGAHAmKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSwPGQARTLFGDHDRfvdTYFKTFKGMYF 497
Cdd:cd05909 298 CSPVISVNTPQSPN-KEGTVGRPLPGMEVKIVSVETHEEVPIGEGGLLLVRG-PNVMLGYLNEPEL---TSFAFGDGWYD 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 498 TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVES-AMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGvEPSEaLR 576
Cdd:cd05909 373 TGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDiLSEILPEDNEVAVVSVPDGRKGEKIVLLTTTTDT-DPSS-LN 450
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 15596084 577 QELKQwvrREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:cd05909 451 DILKN---AGISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
86-616 |
5.94e-22 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 100.88 E-value: 5.94e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 86 LAERGDQVAiiWEgDDPADHQE--ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVF 163
Cdd:PRK06060 10 LAEQASEAG--WY-DRPAFYAAdvVTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFLAN 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 164 GGFSPEALAGRIIDCKSKVVITAdeGVRGGKRTPlkANVDDAltnpetssvqkiivckrtgAEIkwnqhrdvwYDDLMKV 243
Cdd:PRK06060 87 PELHRDDHALAARNTEPALVVTS--DALRDRFQP--SRVAEA-------------------AEL---------MSEAARV 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 244 AGSTCAPkeMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLAN 323
Cdd:PRK06060 135 APGGYEP--MGGDALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGNSVWFPLAT 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 324 GATTILfEGVPNYPDVTrvAKIIDKHKVNILYTAPTairaMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVG 403
Cdd:PRK06060 213 GGSAVI-NSAPVTPEAA--AILSARFGPSVLYGVPN----FFARVIDSCSPDSFRSLRCVVSAGEALELGLAERLMEFFG 285
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 404 QsrCPIVDTWWQTETGACLMTPlpGAHAMKPGSAAKpffgVVPA----LVDNLGNLIEGAAEGNLvildsW---PGQART 476
Cdd:PRK06060 286 G--IPILDGIGSTEVGQTFVSN--RVDEWRLGTLGR----VLPPyeirVVAPDGTTAGPGVEGDL-----WvrgPAIAKG 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 477 LFGDHDRFVdtyfkTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKG 556
Cdd:PRK06060 353 YWNRPDSPV-----ANEGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVRESTGA 427
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 557 QGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK06060 428 STLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
82-615 |
6.20e-22 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 101.78 E-value: 6.20e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGDdpadhqEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:PRK05691 1137 LNEQARQTPERIALVWDGG------SLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVP 1210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITadegvrggkrtplKANVDDALTNPETSSVqkiivckrtgaeIKWNQ-HRDVWyddl 240
Cdd:PRK05691 1211 LDPDYPAERLAYMLADSGVELLLT-------------QSHLLERLPQAEGVSA------------IALDSlHLDSW---- 1261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 241 mkvagSTCAPK-EMGAEDPLFILYTSGSTGKPKGVLHTTGGYL--------VYAsLTHERV--------FDYRPGEVYWc 303
Cdd:PRK05691 1262 -----PSQAPGlHLHGDNLAYVIYTSGSTGQPKGVGNTHAALAerlqwmqaTYA-LDDSDVlmqkapisFDVSVWECFW- 1334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 304 tadigwvtghtyivygPLANGATTILfEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvagaDGSSLRLL 383
Cdd:PRK05691 1335 ----------------PLITGCRLVL-AGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAA----ACTSLRRL 1393
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPINPEAWQWYYETV---------GQSRCPIVDTWWQTETGACLMTPLpgahamkpgsaAKPFFGVVPALVDNLGN 454
Cdd:PRK05691 1394 FSGGEALPAELRNRVLQRLpqvqlhnryGPTETAINVTHWQCQAEDGERSPI-----------GRPLGNVLCRVLDAELN 1462
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 455 LIEGAAEGNLVIldSWPGQARTLFG----DHDRFV-DTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGT 529
Cdd:PRK05691 1463 LLPPGVAGELCI--GGAGLARGYLGrpalTAERFVpDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEP 1540
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 530 AEVESAMVAHPKVAEAAVVgMQHDIKGQGIYVYVTLNSGVE-PSEALRQELKQwvrrEIGPIATPDVIQWAPGLPKTRSG 608
Cdd:PRK05691 1541 EEIQARLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAGQEaEAERLKAALAA----ELPEYMVPAQLIRLDQMPLGPSG 1615
|
....*..
gi 15596084 609 KIMRRIL 615
Cdd:PRK05691 1616 KLDRRAL 1622
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
101-621 |
8.39e-22 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 99.53 E-value: 8.39e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 101 DPADHQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCK 179
Cdd:PLN02574 60 DSSTGFSISYSELQPLVKSMAAGLYHVmGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCS 139
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 180 SKVVITADEGVrgGKRTPLKANVddaLTNPETSSVQKIivcKRTGAEIKWnqhrdvwyddLMKVAGSTCAPKEMGAEDPL 259
Cdd:PLN02574 140 VGLAFTSPENV--EKLSPLGVPV---IGVPENYDFDSK---RIEFPKFYE----------LIKEDFDFVPKPVIKQDDVA 201
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 260 FILYTSGSTGKPKGVLHTTGGYLVYASLT---HERVFDYRPGE-VYWCTADIGWVTGHTYIVYGPLANGATTILFEGVpn 335
Cdd:PLN02574 202 AIMYSSGTTGASKGVVLTHRNLIAMVELFvrfEASQYEYPGSDnVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRF-- 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 336 ypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSsLRLLGSVGEPINPEAWQWYYETVgqSRCPIVDTWWQ 415
Cdd:PLN02574 280 --DASDMVKVIDRFKVTHFPVVPPILMALTKKAKGVCGEVLKS-LKQVSCGAAPLSGKFIQDFVQTL--PHVDFIQGYGM 354
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 416 TETGACLMTPLPGAHAMKPGSAAKPFFGVVPALVD-NLGNLIEGAAEGNLVIldSWPGQARTLFGDHDRFVDTYFKtfKG 494
Cdd:PLN02574 355 TESTAVGTRGFNTEKLSKYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELWI--QGPGVMKGYLNNPKATQSTIDK--DG 430
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 495 MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEA 574
Cdd:PLN02574 431 WLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGSTLSQE 510
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 15596084 575 lrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:PLN02574 511 ---AVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRSLTN 554
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
257-612 |
9.26e-22 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 96.96 E-value: 9.26e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 257 DPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTIL--Fegvp 334
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMekF---- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 335 nypDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINPEAWQwyyETVGQsrcpivdTWW 414
Cdd:cd17637 76 ---DPAEALELIEEEKVTLMGSFPPILSNLLDA--AEKSGVDLSSLRHVLGLDAPETIQRFE---ETTGA-------TFW 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 ----QTETgACLMTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldswpgQARTLFGDHDRFVDTYFK 490
Cdd:cd17637 141 slygQTET-SGLVTLSPYRE--RPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVV------RGPLVFQGYWNLPELTAY 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 491 TFK-GMYFTGDGARRDEDGYYWITGRV--DDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQhDIK-GQGIYVYVTLN 566
Cdd:cd17637 212 TFRnGWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVP-DPKwGEGIKAVCVLK 290
|
330 340 350 360
....*....|....*....|....*....|....*....|....*.
gi 15596084 567 SGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17637 291 PGATLTA---DELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
100-615 |
9.41e-22 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 100.89 E-value: 9.41e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 100 DDPA---DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRII 176
Cdd:PRK10252 473 DAPAladARYQFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLE 552
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 177 DCKSKVVITADEgvrggkrtpLKANVDDAltnPETSSVQkiivckrtgaeikwnqhrdvwYDDLMKVAGStcAPKEMGA- 255
Cdd:PRK10252 553 DARPSLLITTAD---------QLPRFADV---PDLTSLC---------------------YNAPLAPQGA--APLQLSQp 597
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 EDPLFILYTSGSTGKPKGVL--HTTggyLV---------YASLTHERV-------FDYRPGEVYWctadigwvtghtyiv 317
Cdd:PRK10252 598 HHTAYIIFTSGSTGRPKGVMvgQTA---IVnrllwmqnhYPLTADDVVlqktpcsFDVSVWEFFW--------------- 659
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 ygPLANGATTILFEgvpnyPDVTR----VAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPE 393
Cdd:PRK10252 660 --PFIAGAKLVMAE-----PEAHRdplaMQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPAD 732
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 394 AWQWYYETVGqsrCPI----------VD-TWWQTEtgaclmtplPGAHAMKPGSAakpffgvVPA--LVDNLGnliegaa 460
Cdd:PRK10252 733 LCREWQQLTG---APLhnlygpteaaVDvSWYPAF---------GEELAAVRGSS-------VPIgyPVWNTG------- 786
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 461 egnLVILDSW-----PGQARTLF-----------GDHD----RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVL 520
Cdd:PRK10252 787 ---LRILDARmrpvpPGVAGDLYltgiqlaqgylGRPDltasRFIADPFAPGERMYRTGDVARWLDDGAVEYLGRSDDQL 863
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 521 NVSGHRMGTAEVESAMVAHPKVAEA---AVVGMQHDIKGQG---IYVYVTLNSGVEP-SEALRQELkqwvRREIGPIATP 593
Cdd:PRK10252 864 KIRGQRIELGEIDRAMQALPDVEQAvthACVINQAAATGGDarqLVGYLVSQSGLPLdTSALQAQL----RERLPPHMVP 939
|
570 580
....*....|....*....|..
gi 15596084 594 DVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK10252 940 VVLLQLDQLPLSANGKLDRKAL 961
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
99-624 |
1.28e-21 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 99.06 E-value: 1.28e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 99 GDDP---ADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRI 175
Cdd:PRK06155 35 PDRPllvFGGTRWTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHIL 114
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 176 IDCKSKVVITADEGVrggkrTPLKANVDDALTNPETSSVqkiivckrtGAEIKWnqHRDVWYDDLMKVAGSTCAP-KEMG 254
Cdd:PRK06155 115 RNSGARLLVVEAALL-----AALEAADPGDLPLPAVWLL---------DAPASV--SVPAGWSTAPLPPLDAPAPaAAVQ 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 255 AEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHErVFDYRPGEVYWCTADIgWVTGHTYIVYGPLANGATTIL---FE 331
Cdd:PRK06155 179 PGDTAAILYTSGTTGPSKGVCCPHAQFYWWGRNSAE-DLEIGADDVLYTTLPL-FHTNALNAFFQALLAGATYVLeprFS 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 332 GVPNYPDVTRvakiidkHKVNILYTaptaIRAM--MAEGKAAVAGADGSSLRLLGSVGEPinPEAWQWYYETVGqsrCPI 409
Cdd:PRK06155 257 ASGFWPAVRR-------HGATVTYL----LGAMvsILLSQPARESDRAHRVRVALGPGVP--AALHAAFRERFG---VDL 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 410 VDTWWQTETGACLMTPLPGAhamKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGQ-ARTLFGDHDRFVdty 488
Cdd:PRK06155 321 LDGYGSTETNFVIAVTHGSQ---RPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADEPFAfATGYFGMPEKTV--- 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 489 fKTFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVtlns 567
Cdd:PRK06155 395 -EAWRNLWFhTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVFPVPSELGEDEVMAAV---- 469
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 568 GVEPSEALR-QELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK--IAAAEYD 624
Cdd:PRK06155 470 VLRDGTALEpVALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREqgVTADTWD 529
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
90-622 |
1.35e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 98.87 E-value: 1.35e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 90 GDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPE 169
Cdd:PRK08162 32 PDRPAVIHGD------RRRTWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAA 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 170 ALAGRIIDCKSKVVITADEgvrggkrtpLKANVDDALtnpETSSVQKIIVCKRTGAEIKWNQH-RDVWYDDLMKvAGSTC 248
Cdd:PRK08162 106 SIAFMLRHGEAKVLIVDTE---------FAEVAREAL---ALLPGPKPLVIDVDDPEYPGGRFiGALDYEAFLA-SGDPD 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 249 APKEMGAE--DPLFILYTSGSTGKPKGVL-HTTGGYLvyASLTHERVFDYRPGEVYWCTADI----GWvtGHTYIVygpL 321
Cdd:PRK08162 173 FAWTLPADewDAIALNYTSGTTGNPKGVVyHHRGAYL--NALSNILAWGMPKHPVYLWTLPMfhcnGW--CFPWTV---A 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 ANGATTILFEGVpnypDVTRVAKIIDKHKV----------NILYTAPTA--------IRAMMAE--------GKAAVAGA 375
Cdd:PRK08162 246 ARAGTNVCLRKV----DPKLIFDLIREHGVthycgapivlSALINAPAEwragidhpVHAMVAGaappaaviAKMEEIGF 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 376 DGSSLRLLGSVGEPINPEAWQWYYETVGQSRCPIVDTW----WQTETGACLMTPlpgaHAMKPgsaakpffgvVPALVDN 451
Cdd:PRK08162 322 DLTHVYGLTETYGPATVCAWQPEWDALPLDERAQLKARqgvrYPLQEGVTVLDP----DTMQP----------VPADGET 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 452 LGnliEGAAEGNLVIldswpgqartlfgdhdrfvDTYFK-------TFKGMYF-TGDGARRDEDGYYWITGRVDDVLNVS 523
Cdd:PRK08162 388 IG---EIMFRGNIVM-------------------KGYLKnpkateeAFAGGWFhTGDLAVLHPDGYIKIKDRSKDIIISG 445
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 524 GHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPgLP 603
Cdd:PRK08162 446 GENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEE---EIIAHCREHLAGFKVPKAVVFGE-LP 521
|
570
....*....|....*....
gi 15596084 604 KTRSGKIMRRILRKIAAAE 622
Cdd:PRK08162 522 KTSTGKIQKFVLREQAKSL 540
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
91-619 |
1.77e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 98.31 E-value: 1.77e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIwEGDdpadhQEITYRQLHEQVCKFANALRGQDvHRGDVVTIYMPMIPE-----AVVAMLACTRIGaihsvVFGG 165
Cdd:PRK07638 16 NKIAIK-END-----RVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEflqlfAGAAMAGWTCVP-----LDIK 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 166 FSPEALAGRIIDCKSKVVITadegvrggkrtplkanvDDALTNPETSSVQKIIvckrtgaEI-KWNQhrdvwyddLMKVA 244
Cdd:PRK07638 84 WKQDELKERLAISNADMIVT-----------------ERYKLNDLPDEEGRVI-------EIdEWKR--------MIEKY 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 245 GSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTtggylvYASLTHErvFDYRPGEVYWCTADIGWVTG---HTYIVYGPL 321
Cdd:PRK07638 132 LPTYAPIENVQNAPFYMGFTSGSTGKPKAFLRA------QQSWLHS--FDCNVHDFHMKREDSVLIAGtlvHSLFLYGAI 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 322 AN---GATTILFEG-VPNypdvtRVAKIIDKHKVNILYTAPTAIRAMMAEG-------KAAVAGADGSSlrllGSVGE-- 388
Cdd:PRK07638 204 STlyvGQTVHLMRKfIPN-----QVLDKLETENISVMYTVPTMLESLYKENrvienkmKIISSGAKWEA----EAKEKik 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 389 PINPEAWqwYYETVGQSRCPIVdtwwqtetgaclmTPL-PGAHAMKPGSAAKPFFGVVpalvdnlgnlIEGAAEGNLVIL 467
Cdd:PRK07638 275 NIFPYAK--LYEFYGASELSFV-------------TALvDEESERRPNSVGRPFHNVQ----------VRICNEAGEEVQ 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 dswPGQARTLFGDHDRFVDTYF--------KTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAH 539
Cdd:PRK07638 330 ---KGEIGTVYVKSPQFFMGYIiggvlareLNADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEH 406
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 540 PKVAEAAVVGMQHDIKGQgIYVYVTLNSgvepseALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK07638 407 PAVDEIVVIGVPDSYWGE-KPVAIIKGS------ATKQQLKSFCLQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWI 479
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
84-617 |
2.17e-21 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 98.46 E-value: 2.17e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHLAERGDQVAIIWEGDDPADHQEITYRQLHEQVCKFANALRgQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVF 163
Cdd:cd05931 1 RRAAARPDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQ-AVGKPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLP 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 164 GGFSPEA---LAGRIIDCKSKVVITADEgvrggkrtpLKANVDDALTNPETSSVQKIIVCkrtgaeikwnqhrdvwyDDL 240
Cdd:cd05931 80 PPTPGRHaerLAAILADAGPRVVLTTAA---------ALAAVRAFAASRPAAGTPRLLVV-----------------DLL 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 241 MKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGE--VYW--CTADIGWVTGhtyi 316
Cdd:cd05931 134 PDTSAADWPPPSPDPDDIAYLQYTSGSTGTPKGVVVTHRN-LLANVRQIRRAYGLDPGDvvVSWlpLYHDMGLIGG---- 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 VYGPLANGATTILFEGV-----PnypdvTRVAKIIDKHKVNILYtAPT-----AIRAMMAEGKAAVagaDGSSLRLLGSV 386
Cdd:cd05931 209 LLTPLYSGGPSVLMSPAaflrrP-----LRWLRLISRYRATISA-APNfaydlCVRRVRDEDLEGL---DLSSWRVALNG 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 387 GEPINPEAWQWYYETvgqsrcpivdtwwqteTGACLMTPlpgaHAMKPG----------SAAKPFFGVVPALVDnlgnli 456
Cdd:cd05931 280 AEPVRPATLRRFAEA----------------FAPFGFRP----EAFRPSyglaeatlfvSGGPPGTGPVVLRVD------ 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 457 EGAAEGNLVILDSWPGQARTL------FGDHD-RFVDT------------------------YF-------KTFK----- 493
Cdd:cd05931 334 RDALAGRAVAVAADDPAARELvscgrpLPDQEvRIVDPetgrelpdgevgeiwvrgpsvasgYWgrpeataETFGalaat 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 494 --GMYF-TGD-GARRdeDGYYWITGRVDDVLNVSGHRMGTAEVE-SAMVAHPKVAE--AAVVGMQHDIKGQGIYVyVTLN 566
Cdd:cd05931 414 deGGWLrTGDlGFLH--DGELYITGRLKDLIIVRGRNHYPQDIEaTAEEAHPALRPgcVAAFSVPDDGEERLVVV-AEVE 490
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....
gi 15596084 567 SGVEPSE--ALRQELKQWVRREIGpIATPDVIQWAPG-LPKTRSGKIMRRILRK 617
Cdd:cd05931 491 RGADPADlaAIAAAIRAAVAREHG-VAPADVVLVRPGsIPRTSSGKIQRRACRA 543
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
236-617 |
2.61e-21 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 97.83 E-value: 2.61e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 236 WYDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLhTTGGYLVYASLTHERVFDYRPGEVYWCTADI-------- 307
Cdd:PRK07867 132 WADELAAHRDAEPPFRVADPDDLFMLIFTSGTSGDPKAVR-CTHRKVASAGVMLAQRFGLGPDDVCYVSMPLfhsnavma 210
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 308 GWVTGhtyivygpLANGATTIL---FEGVPNYPDVTRV----AKIIDKHKVNILYTAP------TAIRAMMaeGKAAVAG 374
Cdd:PRK07867 211 GWAVA--------LAAGASIALrrkFSASGFLPDVRRYgatyANYVGKPLSYVLATPErpddadNPLRIVY--GNEGAPG 280
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 375 ADGSSLRLLGsvgepinpeawqwyyetvgqsrCPIVDTWWQTETGACLmTPLPGAhamKPGSAAKPFFGVV--------- 445
Cdd:PRK07867 281 DIARFARRFG----------------------CVVVDGFGSTEGGVAI-TRTPDT---PPGALGPLPPGVAivdpdtgte 334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 446 --PALVDNLGNLIEGAAEGNLVILDSwPGQARTLFGDHD----RFVDtyfktfkGMYFTGDGARRDEDGYYWITGRVDDV 519
Cdd:PRK07867 335 cpPAEDADGRLLNADEAIGELVNTAG-PGGFEGYYNDPEadaeRMRG-------GVYWSGDLAYRDADGYAYFAGRLGDW 406
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 520 LNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVE-PSEALRQELKQwvRREIGPIATPDVIQW 598
Cdd:PRK07867 407 MRVDGENLGTAPIERILLRYPDATEVAVYAVPDPVVGDQVMAALVLAPGAKfDPDAFAEFLAA--QPDLGPKQWPSYVRV 484
|
410
....*....|....*....
gi 15596084 599 APGLPKTRSGKIMRRILRK 617
Cdd:PRK07867 485 CAELPRTATFKVLKRQLSA 503
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
109-617 |
3.33e-21 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 97.51 E-value: 3.33e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:PRK06018 41 TYAQIHDRALKVSQALDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTVNPRLFPEQIAWIINHAEDRVVITDLT 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 189 GVrggkrtPLKANVDDALtnpetSSVQKIIVCkrTGAEIKWNQH--RDVWYDDLMKVAGSTCAPKEMGAEDPLFILYTSG 266
Cdd:PRK06018 121 FV------PILEKIADKL-----PSVERYVVL--TDAAHMPQTTlkNAVAYEEWIAEADGDFAWKTFDENTAAGMCYTSG 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 267 STGKPKGVLHTTGGYLVYASLTHER-VFDYRPGEVY-----------WCTADIGWVTGHTYIVYGPLANGATtilfegvp 334
Cdd:PRK06018 188 TTGDPKGVLYSHRSNVLHALMANNGdALGTSAADTMlpvvplfhansWGIAFSAPSMGTKLVMPGAKLDGAS-------- 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 335 nypdvtrVAKIIDKHKVNILYTAPTA----IRAMMAEGKaavagadgsSLRLLGSV--GEPINPEAWQWYYETVGqsrCP 408
Cdd:PRK06018 260 -------VYELLDTEKVTFTAGVPTVwlmlLQYMEKEGL---------KLPHLKMVvcGGSAMPRSMIKAFEDMG---VE 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 409 IVDTWWQTEtgaclMTPLPGAHAMKPGSA--------------AKPFFGVVPALVDNLGNLI--EGAAEGNLVIldSWPG 472
Cdd:PRK06018 321 VRHAWGMTE-----MSPLGTLAALKPPFSklpgdarldvlqkqGYPPFGVEMKITDDAGKELpwDGKTFGRLKV--RGPA 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 473 QARTLFGDHDRFVDTyfktfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQH 552
Cdd:PRK06018 394 VAAAYYRVDGEILDD-----DGFFDTGDVATIDAYGYMRITDRSKDVIKSGGEWISSIDLENLAVGHPKVAEAAVIGVYH 468
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 553 DIKGQGIYVYVTLNSGVEPSealRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK06018 469 PKWDERPLLIVQLKPGETAT---REEILKYMDGKIAKWWMPDDVAFVDAIPHTATGKILKTALRE 530
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
256-609 |
4.79e-21 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 95.14 E-value: 4.79e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 EDPLFILYTSGSTGKPKGVLHTTGGylVYASLTHERVFDYRPGE-VYWCTADIGWVTGHTYIVYGPLANGA--------- 325
Cdd:cd05924 3 ADDLYILYTGGTTGMPKGVMWRQED--IFRMLMGGADFGTGEFTpSEDAHKAAAAAAGTVMFPAPPLMHGTgswtafggl 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 326 ---TTILFEGVPNYPDvtRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETV 402
Cdd:cd05924 81 lggQTVVLPDDRFDPE--EVWRTIEKHKVTSMTIVGDAMARPLIDALRDAGPYDLSSLFAISSGGALLSPEVKQGLLELV 158
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 403 GQSRcpIVDTWWQTETGAcLMTPLPGAHAMKPGSAAKPFFGVVpaLVDNLGNLIEGAAEGnlvildswPGQ-ART----- 476
Cdd:cd05924 159 PNIT--LVDAFGSSETGF-TGSGHSAGSGPETGPFTRANPDTV--VLDDDGRVVPPGSGG--------VGWiARRghipl 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 477 -LFGDHDRFVDTyFKTFKGM--YFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHD 553
Cdd:cd05924 226 gYYGDEAKTAET-FPEVDGVryAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDE 304
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 15596084 554 IKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGK 609
Cdd:cd05924 305 RWGQEVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
79-615 |
1.64e-20 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 94.54 E-value: 1.64e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:cd17645 1 HQLFEEQVERTPDHVAVVDRG------QSLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGA 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyd 238
Cdd:cd17645 75 YVPIDPDYPGERIAYMLADSSAKILLT----------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 dlmkvagstcapkemGAEDPLFILYTSGSTGKPKGVL--HTTggyLVYASLTHERVFDYRPGEVYWCTADIGWvTGHTYI 316
Cdd:cd17645 102 ---------------NPDDLAYVIYTSGSTGLPKGVMieHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWE 162
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 VYGPLANGATTILFEGVPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAegkaavagADGSSLRLLGSVGEPINPEAWQ 396
Cdd:cd17645 163 IFPHLTAGAALHVVPSERRL-DLDALNDYFNQEGITISFLPTGAAEQFMQ--------LDNQSLRVLLTGGDKLKKIERK 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 397 WY--YETVGQSRCPIVDTwwqtetgaclMTPLPGAHAMKPgsAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSwpGQA 474
Cdd:cd17645 234 GYklVNNYGPTENTVVAT----------SFEIDKPYANIP--IGKPIDNTRVYILDEALQLQPIGVAGELCIAGE--GLA 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFGDHD----RFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGM 550
Cdd:cd17645 300 RGYLNRPEltaeKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAK 379
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 551 QHDIKGQGIYVYVTLNSGVEPsealrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17645 380 EDADGRKYLVAYVTAPEEIPH-----EELREWLKNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
244-616 |
2.24e-20 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 94.67 E-value: 2.24e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 244 AGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGylVYASLthervfDYRPGEVYWcTADIGWVTG------HTYI- 316
Cdd:PRK07787 116 ARSWHRYPEPDPDAPALIVYTSGTTGPPKGVVLSRRA--IAADL------DALAEAWQW-TADDVLVHGlplfhvHGLVl 186
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 -VYGPLANGATTILFegvpNYPDVTRVAKIIDKhKVNILYTAPTAIRAMMAEGKAAVAGadgSSLRLLGSVGEPINPEAW 395
Cdd:PRK07787 187 gVLGPLRIGNRFVHT----GRPTPEAYAQALSE-GGTLYFGVPTVWSRIAADPEAARAL---RGARLLVSGSAALPVPVF 258
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 396 QWYYETVGQsrcPIVDTWWQTETGACLMTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLI--EGAAEGNLVIldswpgQ 473
Cdd:PRK07787 259 DRLAALTGH---RPVERYGMTETLITLSTRADGER--RPGWVGLPLAGVETRLVDEDGGPVphDGETVGELQV------R 327
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 474 ARTLFGDH----DRFVDTYfkTFKGMYFTGDGARRDEDGYYWITGRVD-DVLNVSGHRMGTAEVESAMVAHPKVAEAAVV 548
Cdd:PRK07787 328 GPTLFDGYlnrpDATAAAF--TADGWFRTGDVAVVDPDGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVV 405
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 549 GMQHDIKGQGIYVYVTLNSGVEPsealrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK07787 406 GVPDDDLGQRIVAYVVGADDVAA-----DELIDFVAQQLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
79-622 |
3.54e-20 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 96.00 E-value: 3.54e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:PRK12467 1577 HQLIEDQAAATPEAVALVFGE------QELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGA 1650
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITADEGVrggKRTPLKANVDDALTNPEtssvqkiivckrtgaeikwnqhrDVWYD 238
Cdd:PRK12467 1651 YVPLDPEYPRERLAYMIEDSGIELLLTQSHLQ---ARLPLPDGLRSLVLDQE-----------------------DDWLE 1704
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 dlmkvAGSTCAPKEMGAEDPL-FILYTSGSTGKPKGVLHTTGGYLvyasltheRVFDYRpGEVYWCTADIGWVTGHTYI- 316
Cdd:PRK12467 1705 -----GYSDSNPAVNLAPQNLaYVIYTSGSTGRPKGAGNRHGALV--------NRLCAT-QEAYQLSAADVVLQFTSFAf 1770
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 ------VYGPLANGATTILFE-GVPNYPDvtRVAKIIDKHKVNILYTAPTAIRAMMaEGKAAVAGAdgSSLRLLGSVGEP 389
Cdd:PRK12467 1771 dvsvweLFWPLINGARLVIAPpGAHRDPE--QLIQLIERQQVTTLHFVPSMLQQLL-QMDEQVEHP--LSLRRVVCGGEA 1845
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 390 INPEAWQWYYETVGQSRcpIVDTWWQTETgACLMTPLPGAHAMKPGSAAKPFFGVVPAL----VDNLGNLIEGAAEGNLV 465
Cdd:PRK12467 1846 LEVEALRPWLERLPDTG--LFNLYGPTET-AVDVTHWTCRRKDLEGRDSVPIGQPIANLstyiLDASLNPVPIGVAGELY 1922
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 466 IldSWPGQARtlfGDH-------DRFVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMV 537
Cdd:PRK12467 1923 L--GGVGLAR---GYLnrpaltaERFVADPFGTVGSrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLR 1997
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 538 AHPKVAEAAVVGmQHDIKGQGIYVYVT-----LNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:PRK12467 1998 EQGGVREAVVIA-QDGANGKQLVAYVVptdpgLVDDDEAQVALRAILKNHLKASLPEYMVPAHLVFLARMPLTPNGKLDR 2076
|
570
....*....|
gi 15596084 613 RILRKIAAAE 622
Cdd:PRK12467 2077 KALPAPDASE 2086
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
108-616 |
3.72e-20 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 94.32 E-value: 3.72e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVI--- 184
Cdd:PRK07059 49 ITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIVvle 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 185 ----TADEGVRggkRTPLK----ANVDD------ALTNPETSSVQKIIVCKRTGAEIKWNqhrdvwyDDLMKVAGSTCAP 250
Cdd:PRK07059 129 nfatTVQQVLA---KTAVKhvvvASMGDllgfkgHIVNFVVRRVKKMVPAWSLPGHVRFN-------DALAEGARQTFKP 198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 251 KEMGAEDPLFILYTSGSTGKPKGVLHTTGGYL---VYASLTHERVFDYRPGE---VYWCTADIGWVTGHTYIVYGPLANG 324
Cdd:PRK07059 199 VKLGPDDVAFLQYTGGTTGVSKGATLLHRNIVanvLQMEAWLQPAFEKKPRPdqlNFVCALPLYHIFALTVCGLLGMRTG 278
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 325 ATTILfegVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMaeGKAAVAGADGSSLRL-LG---SVGEPInpeAWQWYYE 400
Cdd:PRK07059 279 GRNIL---IPNPRDIPGFIKELKKYQVHIFPAVNTLYNALL--NNPDFDKLDFSKLIVaNGggmAVQRPV---AERWLEM 350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 401 TvgqsRCPIVDTWWQTETGACLmTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLI------EGAAEGNLVILDSW--PG 472
Cdd:PRK07059 351 T----GCPITEGYGLSETSPVA-TCNPVDATEFSGTIGLPLPSTEVSIRDDDGNDLplgepgEICIRGPQVMAGYWnrPD 425
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 473 Q-ARTLFGDhdrfvdTYFKTfkgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQ 551
Cdd:PRK07059 426 EtAKVMTAD------GFFRT-------GDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEVAAVGVP 492
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15596084 552 HDIKGQGIYVYVtlnsgVEPSEAL-RQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK07059 493 DEHSGEAVKLFV-----VKKDPALtEEDVKAFCKERLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
108-616 |
9.75e-20 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 93.29 E-value: 9.75e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsVVFGG--FSPEALAGRIIDCKSK-VV 183
Cdd:PRK05677 50 LTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLI--VVNTNplYTAREMEHQFNDSGAKaLV 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITAD-----EGV--RGGKRTPLKANVDD-------ALTNPETSSVQKIIVCKRTGAEIKWNqhrdvwyDDLMKVAGSTCA 249
Cdd:PRK05677 128 CLANmahlaEKVlpKTGVKHVIVTEVADmlpplkrLLINAVVKHVKKMVPAYHLPQAVKFN-------DALAKGAGQPVT 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 250 PKEMGAEDPLFILYTSGSTGKPKGvlhttggylvyASLTHERVFdyrpGEVYWCTADIGWVTGH-TYIVYGPLA------ 322
Cdd:PRK05677 201 EANPQADDVAVLQYTGGTTGVAKG-----------AMLTHRNLV----ANMLQCRALMGSNLNEgCEILIAPLPlyhiya 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 323 ----------NGATTILfegVPNYPDVTRVAKIIDKHK------VNILYTA---PTAIRAMmaegkaavagaDGSSLRLL 383
Cdd:PRK05677 266 ftfhcmammlIGNHNIL---ISNPRDLPAMVKELGKWKfsgfvgLNTLFVAlcnNEAFRKL-----------DFSALKLT 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPINPEAWQWYYETVGqsrCPIVDTWWQTETgACLMTPLPgAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGN 463
Cdd:PRK05677 332 LSGGMALQLATAERWKEVTG---CAICEGYGMTET-SPVVSVNP-SQAIQVGTIGIPVPSTLCKVIDDDGNELPLGEVGE 406
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 464 L------VILDSWPGQARTlfgdhDRFVDTyfktfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMV 537
Cdd:PRK05677 407 LcvkgpqVMKGYWQRPEAT-----DEILDS-----DGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLA 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 538 AHPKVAEAAVVGMQHDIKGQGIYVYVTlnsgVEPSEALRQE-LKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK05677 477 ALPGVLQCAAIGVPDEKSGEAIKVFVV----VKPGETLTKEqVMEHMRANLTGYKVPKAVEFRDELPTTNVGKILRRELR 552
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
256-616 |
1.46e-19 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 90.41 E-value: 1.46e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 EDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVfDYRPGEV-------YWCtadIGWVTGhtyiVYGPLANGATTI 328
Cdd:cd05917 2 DDVINIQFTSGTTGSPKGATLTHHNIVNNGYFIGERL-GLTEQDRlcipvplFHC---FGSVLG----VLACLTHGATMV 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 329 LFEgvPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKaaVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRCP 408
Cdd:cd05917 74 FPS--PSF-DPLAVLEAIEKEKCTALHGVPTMFIAELEHPD--FDKFDLSSLRTGIMAGAPCPPELMKRVIEVMNMKDVT 148
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 409 IVdtWWQTETGA-CLMTpLPGA----------HAMkPGSAAK---PFFGVVPALvdnlGNLIEGAAEGNLVILDSWPGQA 474
Cdd:cd05917 149 IA--YGMTETSPvSTQT-RTDDsiekrvntvgRIM-PHTEAKivdPEGGIVPPV----GVPGELCIRGYSVMKGYWNDPE 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RT-LFGDHDRFvdtyfktfkgmYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHD 553
Cdd:cd05917 221 KTaEAIDGDGW-----------LHTGDLAVMDEDGYCRIVGRIKDMIIRGGENIYPREIEEFLHTHPKVSDVQVVGVPDE 289
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15596084 554 IKGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:cd05917 290 RYGEEVCAWIRLKEGAELTEE---DIKAYCKGKIAHYKVPRYVFFVDEFPLTVSGKIQKFKLR 349
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
235-615 |
3.39e-19 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 92.92 E-value: 3.39e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 235 VWyDDLMKVAGSTCAPKEMGAEDPL-FILYTSGSTGKPKGVLHTTGGYL-------VYASLTHERVFDYRPGEVYwctaD 306
Cdd:PRK05691 3848 VW-EEVQAGEVASHNPGIYSGPDNLaYVIYTSGSTGLPKGVMVEQRGMLnnqlskvPYLALSEADVIAQTASQSF----D 3922
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 307 IG-WvtghtYIVYGPLANGATTIlfegVPN--YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGadgssLRLL 383
Cdd:PRK05691 3923 ISvW-----QFLAAPLFGARVEI----VPNaiAHDPQGLLAHVQAQGITVLESVPSLIQGMLAEDRQALDG-----LRWM 3988
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPINPE-AWQWY--YETVGqsrcpivdtwwqtetgaclmtpLPGAHAMKPGSAAKPFFGVVPA------------- 447
Cdd:PRK05691 3989 LPTGEAMPPElARQWLqrYPQIG----------------------LVNAYGPAECSDDVAFFRVDLAstrgsylpigspt 4046
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 448 ------LVDNLGNLIEGAAEGNLVILDSwpGQARTLFGDHDR----FVDTYFKTF-KGMYFTGDGARRDEDGYYWITGRV 516
Cdd:PRK05691 4047 dnnrlyLLDEALELVPLGAVGELCVAGT--GVGRGYVGDPLRtalaFVPHPFGAPgERLYRTGDLARRRSDGVLEYVGRI 4124
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 517 DDVLNVSGHRMGTAEVESAMVAHPKVAEAAvVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVI 596
Cdd:PRK05691 4125 DHQVKIRGYRIELGEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYLVPHQTVLAQGALLERIKQRLRAELPDYMVPLHW 4203
|
410
....*....|....*....
gi 15596084 597 QWAPGLPKTRSGKIMRRIL 615
Cdd:PRK05691 4204 LWLDRLPLNANGKLDRKAL 4222
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
82-551 |
3.40e-19 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 91.47 E-value: 3.40e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSV 161
Cdd:PRK08279 43 FEEAAARHPDRPALLFED------QSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVAL 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEALAGRIIDCKSKVVITADE------GVRGGKRTPLKANVDDALTNPETSSvqkiivckrtgaeikwnqhrdv 235
Cdd:PRK08279 117 LNTQQRGAVLAHSLNLVDAKHLIVGEElveafeEARADLARPPRLWVAGGDTLDDPEG---------------------- 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 236 wYDDLMKVAG--STCAP---KEMGAEDPLFILYTSGSTGKPKGvlhttggylvyASLTHERVF----------DYRPGEV 300
Cdd:PRK08279 175 -YEDLAAAAAgaPTTNPasrSGVTAKDTAFYIYTSGTTGLPKA-----------AVMSHMRWLkamggfggllRLTPDDV 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 301 YWCTADIGWVTGHTYIVYGPLANGATTIL---FEGVPNYPDVTRvakiidkHKVnilyTAPTAI----RAMMAEgkAAVA 373
Cdd:PRK08279 243 LYCCLPLYHNTGGTVAWSSVLAAGATLALrrkFSASRFWDDVRR-------YRA----TAFQYIgelcRYLLNQ--PPKP 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 374 GADGSSLRLLgsVGEPINPEAWQWYYETVGQSRcpIVDTWWQTE--TGACLMTPLPGAHAMKPGSAAKPFfgvvpALV-- 449
Cdd:PRK08279 310 TDRDHRLRLM--IGNGLRPDIWDEFQQRFGIPR--ILEFYAASEgnVGFINVFNFDGTVGRVPLWLAHPY-----AIVky 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 450 ---------DNLGNLIE-GAAE-GNLV--ILDSWP---------GQARTLfgdHDRFV--DTYFKtfkgmyfTGDGARRD 505
Cdd:PRK08279 381 dvdtgepvrDADGRCIKvKPGEvGLLIgrITDRGPfdgytdpeaSEKKIL---RDVFKkgDAWFN-------TGDLMRDD 450
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|.
gi 15596084 506 EDGYYWITGRVDDVL-----NVSghrmgTAEVESAMVAHPKVAEAAVVGMQ 551
Cdd:PRK08279 451 GFGHAQFVDRLGDTFrwkgeNVA-----TTEVENALSGFPGVEEAVVYGVE 496
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
109-621 |
5.25e-19 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 90.85 E-value: 5.25e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITADE 188
Cdd:PLN03102 41 TWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFVDRS 120
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 189 GVRGGKRTPLKANVDDALTNPETSSVQKIIVCKRTGAEikwnqhrDVWYDDLMK--------VAGSTCAPKEmgaEDPLF 260
Cdd:PLN03102 121 FEPLAREVLHLLSSEDSNLNLPVIFIHEIDFPKRPSSE-------ELDYECLIQrgeptpslVARMFRIQDE---HDPIS 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 261 ILYTSGSTGKPKGVLHT-TGGYLVYASLtherVFDYRPG--EVYWCTADIGWVTGHTYiVYGPLANGATTILFEGVpNYP 337
Cdd:PLN03102 191 LNYTSGTTADPKGVVIShRGAYLSTLSA----IIGWEMGtcPVYLWTLPMFHCNGWTF-TWGTAARGGTSVCMRHV-TAP 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 338 DVTrvaKIIDKHKVNILYTAPTAIRAMMaEGKAAVAGADGSSLRLLGSVGEPinPEAWQWYYETVG----------QSRC 407
Cdd:PLN03102 265 EIY---KNIEMHNVTHMCCVPTVFNILL-KGNSLDLSPRSGPVHVLTGGSPP--PAALVKKVQRLGfqvmhaygltEATG 338
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 408 PIVDTWWQTEtgaclMTPLPGAHAMK-PGSAAKPFFGVVPALVDNLGNL----IEGAAEGNLVILDSwpgqarTLFGDHD 482
Cdd:PLN03102 339 PVLFCEWQDE-----WNRLPENQQMElKARQGVSILGLADVDVKNKETQesvpRDGKTMGEIVIKGS------SIMKGYL 407
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 483 RFVDTYFKTFK-GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYV 561
Cdd:PLN03102 408 KNPKATSEAFKhGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWGETPCA 487
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 562 YVTLNSGVEPSEALRQELK-------QWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:PLN03102 488 FVVLEKGETTKEDRVDKLVtrerdliEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDIAKG 554
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
103-613 |
6.97e-19 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 90.33 E-value: 6.97e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 103 ADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsVVfggfsPealagriIDCKSKV 182
Cdd:PRK05852 39 ADRIAISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLV--VV-----P-------LDPALPI 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 183 VITADEGVRGGKRTPLKANVDDALTNPETSSVQKIIVcKRTGAEIKWNQHRDVWYDDLMKVAGSTCAPKEMGAEDPLfIL 262
Cdd:PRK05852 105 AEQRVRSQAAGARVVLIDADGPHDRAEPTTRWWPLTV-NVGGDSGPSGGTLSVHLDAATEPTPATSTPEGLRPDDAM-IM 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 263 YTSGSTGKPKGVLHTTGGYlvyASLTHERVFDYRPGEVYWCTADIGWVTGHTYI--VYGPLANGATTIL-----FEGVPN 335
Cdd:PRK05852 183 FTGGTTGLPKMVPWTHANI---ASSVRAIITGYRLSPRDATVAVMPLYHGHGLIaaLLATLASGGAVLLpargrFSAHTF 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 336 YPDVTRVAKIidkhkvniLYTA-PTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGqsrCPIVDTWW 414
Cdd:PRK05852 260 WDDIKAVGAT--------WYTAvPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAETAQALQTEFA---APVVCAFG 328
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 415 QTE-TGACLMTPLPGAH-----AMKPGSAAKPFfGVVPALVDNLGNLIEGAAEGNLVIldSWPGQARTLFGDHD----RF 484
Cdd:PRK05852 329 MTEaTHQVTTTQIEGIGqtenpVVSTGLVGRST-GAQIRIVGSDGLPLPAGAVGEVWL--RGTTVVRGYLGDPTitaaNF 405
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 485 VDTYFKTfkgmyftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVT 564
Cdd:PRK05852 406 TDGWLRT-------GDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPDQLYGEAVAAVIV 478
|
490 500 510 520
....*....|....*....|....*....|....*....|....*....
gi 15596084 565 LNSGVEPSealRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRR 613
Cdd:PRK05852 479 PRESAPPT---AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
100-621 |
2.51e-18 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 88.78 E-value: 2.51e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 100 DDPADH---QEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRI 175
Cdd:PRK08751 40 DRPAYHsfgKTITYREADQLVEQFAAYLLGElQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQL 119
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 176 IDCKSKVVITAD------EGVRGGkrTPLK----ANVDDALTNPETSSVQKII-VCKRTGAEIKWNqhRDVWYDDLMKVA 244
Cdd:PRK08751 120 IDSGASVLVVIDnfgttvQQVIAD--TPVKqvitTGLGDMLGFPKAALVNFVVkYVKKLVPEYRIN--GAIRFREALALG 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 245 GSTCAPK-EMGAEDPLFILYTSGSTGKPKGvlhttggylvyASLTHERVFDYRPGEVYWCTADIGWVTGHT--------Y 315
Cdd:PRK08751 196 RKHSMPTlQIEPDDIAFLQYTGGTTGVAKG-----------AMLTHRNLVANMQQAHQWLAGTGKLEEGCEvvitalplY 264
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 316 IVYGPLANGATTILFEGVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAE--GKAAVAGADGSSLRLLGSVGEPINPE 393
Cdd:PRK08751 265 HIFALTANGLVFMKIGGCNHLISNPRDMPGFVKELKKTRFTAFTGVNTLFNGllNTPGFDQIDFSSLKMTLGGGMAVQRS 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 394 AWQWYYETVGqsrCPIVDTWWQTET--GAClMTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldswp 471
Cdd:PRK08751 345 VAERWKQVTG---LTLVEAYGLTETspAAC-INPLTLKE--YNGSIGLPIPSTDACIKDDAGTVLAIGEIGELCI----- 413
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 472 GQARTLFGDHDRFVDT-YFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGM 550
Cdd:PRK08751 414 KGPQVMKGYWKRPEETaKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGV 493
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15596084 551 QHDIKGQGIYVYVtlnsgVEPSEALR-QELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:PRK08751 494 PDEKSGEIVKVVI-----VKKDPALTaEDVKAHARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDAAKA 560
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
257-612 |
6.98e-18 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 85.15 E-value: 6.98e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 257 DPLFILYTSGSTGKPKGVLHTTGGYLvyaslthervfdyrpgEVYWCTADIGWVTGHTYI-VYGPLA-----NGATTILF 330
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWI----------------ESFVCNEDLFNISGEDAIlAPGPLShslflYGAISALY 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 331 EG----VPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvagadgSSLRLLGSVGEPINPEAWQWYYETVGQSR 406
Cdd:cd17633 65 LGgtfiGQRKFNPKSWIRKINQYNATVIYLVPTMLQALARTLEPE------SKIKSIFSSGQKLFESTKKKLKNIFPKAN 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 407 cpIVDTWWQTETGacLMTPLPGAHAMKPGSAAKPFFGVvpalvdnlgnliegaaegNLVILDSWPGQARTLFGDHDRFVD 486
Cdd:cd17633 139 --LIEFYGTSELS--FITYNFNQESRPPNSVGRPFPNV------------------EIEIRNADGGEIGKIFVKSEMVFS 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 487 TY----FKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQgIYVY 562
Cdd:cd17633 197 GYvrggFSNPDGWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGE-IAVA 275
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|
gi 15596084 563 VtlnsgVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17633 276 L-----YSGDKLTYKQLKRFLKQKLSRYEIPKKIIFVDSLPYTSSGKIAR 320
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
261-612 |
7.86e-18 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 85.25 E-value: 7.86e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 261 ILYTSGSTGKPKGVLHTtggylvyaslthervfdYRPG-EVYWCTADIGWVT-GHTYIVYGPLAN------GATTILFEG 332
Cdd:cd17638 5 IMFTSGTTGRSKGVMCA-----------------HRQTlRAAAAWADCADLTeDDRYLIINPFFHtfgykaGIVACLLTG 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 333 VPNYP----DVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSRcp 408
Cdd:cd17638 68 ATVVPvavfDVDAILEAIERERITVLPGPPTLFQSLLDH--PGRKKFDLSSLRAAVTGAATVPVELVRRMRSELGFET-- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 409 IVDTWWQTETGACLMTPlPGAHAMKPGS-AAKPFFGVVPALVDNLGNLIEGAaegNLVI--LDSWPGQARTLFGDhdrfv 485
Cdd:cd17638 144 VLTAYGLTEAGVATMCR-PGDDAETVATtCGRACPGFEVRIADDGEVLVRGY---NVMQgyLDDPEATAEAIDAD----- 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 486 dtyfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTL 565
Cdd:cd17638 215 --------GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVGKAFVVA 286
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 15596084 566 NSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMR 612
Cdd:cd17638 287 RPGVTLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
82-625 |
1.03e-17 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 86.65 E-value: 1.03e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHS 160
Cdd:PRK08974 29 FEQAVARYADQPAFINMG------EVMTFRKLEERSRAFAAYLQNGlGLKKGDRVALMMPNLLQYPIALFGILRAGMIVV 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 161 VVFGGFSPEALAGRIIDCKSKVVI-------TADEGVrggKRTPLK----ANVDDALTNPETSSVQkiIVCKRTGAEI-K 228
Cdd:PRK08974 103 NVNPLYTPRELEHQLNDSGAKAIVivsnfahTLEKVV---FKTPVKhvilTRMGDQLSTAKGTLVN--FVVKYIKRLVpK 177
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 229 WNQHRDVWYDDLMKVAGSTCAPK-EMGAEDPLFILYTSGSTGKPKGvlhttggylvyASLTHERVFdyrpgevywctADI 307
Cdd:PRK08974 178 YHLPDAISFRSALHKGRRMQYVKpELVPEDLAFLQYTGGTTGVAKG-----------AMLTHRNML-----------ANL 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 308 GWVTGhtyiVYGPLANGATTILFEGVPNYPDVTRVAK---IIDKHKVNILYTAPTAIRAMMAEGK----AAVAGA----- 375
Cdd:PRK08974 236 EQAKA----AYGPLLHPGKELVVTALPLYHIFALTVNcllFIELGGQNLLITNPRDIPGFVKELKkypfTAITGVntlfn 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 376 -----------DGSSLRLLGSVGEPIN---PEAWQwyyETVGqsrCPIVDTWWQTETgACLMTPLPGAHAMKPGSAAKPf 441
Cdd:PRK08974 312 allnneefqelDFSSLKLSVGGGMAVQqavAERWV---KLTG---QYLLEGYGLTEC-SPLVSVNPYDLDYYSGSIGLP- 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 442 fgvVPA----LVDNLGNLIEGAAEGNL------VILDSW--PGQARTLFGDhdrfvdtyfktfkGMYFTGDGARRDEDGY 509
Cdd:PRK08974 384 ---VPSteikLVDDDGNEVPPGEPGELwvkgpqVMLGYWqrPEATDEVIKD-------------GWLATGDIAVMDEEGF 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 510 YWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVtlnsgVEPSEAL-RQELKQWVRREIG 588
Cdd:PRK08974 448 LRIVDRKKDMILVSGFNVYPNEIEDVVMLHPKVLEVAAVGVPSEVSGEAVKIFV-----VKKDPSLtEEELITHCRRHLT 522
|
570 580 590
....*....|....*....|....*....|....*..
gi 15596084 589 PIATPDVIQWAPGLPKTRSGKIMRRILRKIAAAEYDT 625
Cdd:PRK08974 523 GYKVPKLVEFRDELPKSNVGKILRRELRDEARAKVDN 559
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
256-615 |
1.46e-17 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 85.95 E-value: 1.46e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 EDPLFILYTSGSTGKPKGVL--------HTTGGYLVYASLTHERV-------FDYRPGEVYwctadIGWVTGHTYIVygp 320
Cdd:cd17644 106 ENLAYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSSDRVlqfasiaFDVAAEEIY-----VTLLSGATLVL--- 177
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 321 langattilfegVPN--YPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADgSSLRLLGSVGEPINPEAWQWY 398
Cdd:cd17644 178 ------------RPEemRSSLEDFVQYIQQWQLTVLSLPPAYWHLLVLELLLSTIDLP-SSLRLVIVGGEAVQPELVRQW 244
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 399 YETVGQsRCPIVDTWWQTE----TGACLMTPLPGAHAMKPgSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIldSWPGQA 474
Cdd:cd17644 245 QKNVGN-FIQLINVYGPTEatiaATVCRLTQLTERNITSV-PIGRPIANTQVYILDENLQPVPVGVPGELHI--GGVGLA 320
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFGDHD----RFVDTYF--KTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVV 548
Cdd:cd17644 321 RGYLNRPEltaeKFISHPFnsSESERLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIELGEIEAVLSQHNDVKTAVVI 400
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 15596084 549 gMQHDIKGQGIYV-YVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17644 401 -VREDQPGNKRLVaYIVPHYEESPSTV---ELRQFLKAKLPDYMIPSAFVVLEELPLTPNGKIDRRAL 464
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
109-619 |
4.28e-17 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 84.90 E-value: 4.28e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT--- 185
Cdd:PLN02479 47 TWAQTYQRCRRLASALAKRSIGPGSTVAVIAPNIPAMYEAHFGVPMAGAVVNCVNIRLNAPTIAFLLEHSKSEVVMVdqe 126
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 ----ADEGVR-------GGKRTPLKANVDDALTNPEtsSVQKIIvckRTGA------------EIKWNQHRDVWyddlmk 242
Cdd:PLN02479 127 fftlAEEALKilaekkkSSFKPPLLIVIGDPTCDPK--SLQYAL---GKGAieyekfletgdpEFAWKPPADEW------ 195
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 243 vagstcapkemgaeDPLFILYTSGSTGKPKGV-LHTTGGYLvyASLTHERVFDYRPGEVY-----------WC-TADIGW 309
Cdd:PLN02479 196 --------------QSIALGYTSGTTASPKGVvLHHRGAYL--MALSNALIWGMNEGAVYlwtlpmfhcngWCfTWTLAA 259
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 310 VTGhTYI---------VYGPLANGATT------ILFEGVPNYPDVTRVAKIidKHKVNILyTA----PTAIRAMMAEGKA 370
Cdd:PLN02479 260 LCG-TNIclrqvtakaIYSAIANYGVThfcaapVVLNTIVNAPKSETILPL--PRVVHVM-TAgaapPPSVLFAMSEKGF 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 371 AVAGADGSSlrllgsvgEPINPE---AWQWYYET-----------------VGQSRCPIVDTwwqtetgaclmtplpgaH 430
Cdd:PLN02479 336 RVTHTYGLS--------ETYGPStvcAWKPEWDSlppeeqarlnarqgvryIGLEGLDVVDT-----------------K 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 431 AMKPgsaakpffgvVPALVDNLGNLIegaAEGNLVIldswPGQARTLFGDHDRFVDtyfktfkGMYFTGDGARRDEDGYY 510
Cdd:PLN02479 391 TMKP----------VPADGKTMGEIV---MRGNMVM----KGYLKNPKANEEAFAN-------GWFHSGDLGVKHPDGYI 446
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 511 WITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSE--ALRQELKQWVRREIG 588
Cdd:PLN02479 447 EIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSDeaALAEDIMKFCRERLP 526
|
570 580 590
....*....|....*....|....*....|.
gi 15596084 589 PIATPDVIQWAPgLPKTRSGKIMRRILRKIA 619
Cdd:PLN02479 527 AYWVPKSVVFGP-LPKTATGKIQKHVLRAKA 556
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
260-615 |
8.32e-17 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 85.22 E-value: 8.32e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 260 FILYTSGSTGKPKGVLHTTGGYLVYASLTHERvFDYRPG--EVYWCTADIGWVTGHTYIvygPLANGATTILfeGVPNYP 337
Cdd:PRK05691 2337 YLIYTSGSTGKPKGVVVSHGEIAMHCQAVIER-FGMRADdcELHFYSINFDAASERLLV---PLLCGARVVL--RAQGQW 2410
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 338 DVTRVAKIIDKHKVNILYTAPTaIRAMMAEGKAAvaGADGSSLRLLGSVGEPINPEAWQwyyeTVGQSRCP--IVDTWWQ 415
Cdd:PRK05691 2411 GAEEICQLIREQQVSILGFTPS-YGSQLAQWLAG--QGEQLPVRMCITGGEALTGEHLQ----RIRQAFAPqlFFNAYGP 2483
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 416 TETgacLMTPL--PGAHAMKPGSAAKPFFGVVPALV-----DNLGNLIEGAAeGNLVIldswpGQARTLFGDHDR----- 483
Cdd:PRK05691 2484 TET---VVMPLacLAPEQLEEGAASVPIGRVVGARVayildADLALVPQGAT-GELYV-----GGAGLAQGYHDRpglta 2554
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 484 --FVDTYFKTFKG-MYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQ--G 558
Cdd:PRK05691 2555 erFVADPFAADGGrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEHPAVREAVVLALDTPSGKQlaG 2634
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 559 IYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK05691 2635 YLVSAVAGQDDEAQAALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
237-616 |
2.36e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 82.38 E-value: 2.36e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 237 YDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERvFDYRPGEVYWCTADI--------G 308
Cdd:PRK13388 131 YAELVAAAGALTPHREVDAMDPFMLIFTSGTTGAPKAVRCSHGRLAFAGRALTER-FGLTRDDVCYVSMPLfhsnavmaG 209
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 309 WvtghtyivyGP-LANGATTIL---FEGVPNYPDVTR--------VAKIIDkhkvnilYTAPTAIRAMMAEGKAAVAgad 376
Cdd:PRK13388 210 W---------APaVASGAAVALpakFSASGFLDDVRRygatyfnyVGKPLA-------YILATPERPDDADNPLRVA--- 270
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 377 gsslrlLGSVGEPINPEAWQWYYetvgqsRCPIVDTWWQTEtGACLMTPLPGAhamKPGSAAKPFFGVV----------- 445
Cdd:PRK13388 271 ------FGNEASPRDIAEFSRRF------GCQVEDGYGSSE-GAVIVVREPGT---PPGSIGRGAPGVAiynpetlteca 334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 446 PALVDNLGNLIEGA-AEGNLVILdswpgQARTLF--------GDHDRFVDtyfktfkGMYFTGDGARRDEDGYYWITGRV 516
Cdd:PRK13388 335 VARFDAHGALLNADeAIGELVNT-----AGAGFFegyynnpeATAERMRH-------GMYWSGDLAYRDADGWIYFAGRT 402
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 517 DDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNsgvEPSEALRQELKQWV--RREIGPIATPD 594
Cdd:PRK13388 403 ADWMRVDGENLSAAPIERILLRHPAINRVAVYAVPDERVGDQVMAALVLR---DGATFDPDAFAAFLaaQPDLGTKAWPR 479
|
410 420
....*....|....*....|..
gi 15596084 595 VIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK13388 480 YVRIAADLPSTATNKVLKRELI 501
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
82-621 |
2.92e-16 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 81.96 E-value: 2.92e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAerGDQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsV 161
Cdd:PRK10946 31 LTRHAA--SDAIAVI------CGERQFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--P 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPE-----ALAGRIidcKSKVVItadegvrGGKRTPLKANvDDALT--NPETSSVQKIIVCKRTGAeikwnqhRD 234
Cdd:PRK10946 101 VNALFSHQrselnAYASQI---EPALLI-------ADRQHALFSD-DDFLNtlVAEHSSLRVVLLLNDDGE-------HS 162
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 235 VwyDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYL--VYASlthervfdyrpgevywctADIGWVTG 312
Cdd:PRK10946 163 L--DDAINHPAEDFTATPSPADEVAFFQLSGGSTGTPKLIPRTHNDYYysVRRS------------------VEICGFTP 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 313 HT-YIVYGPLAN-------GATTILFEG-----VPNyPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSS 379
Cdd:PRK10946 223 QTrYLCALPAAHnypmsspGALGVFLAGgtvvlAPD-PSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSRAQLAS 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 380 LRLLgSVGepinpeawqwyyetvgqsrcpivdtwwqtetGACLMTPLpgahamkpgsAAKpffgvVPALvdnLGNLIE-- 457
Cdd:PRK10946 302 LKLL-QVG-------------------------------GARLSETL----------ARR-----IPAE---LGCQLQqv 331
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 458 -GAAEG--NLVILDSWP-----GQARTLFGDHD-RFVDT-----------------------YFKT-------F--KGMY 496
Cdd:PRK10946 332 fGMAEGlvNYTRLDDSDeriftTQGRPMSPDDEvWVADAdgnplpqgevgrlmtrgpytfrgYYKSpqhnasaFdaNGFY 411
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 497 FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSeALR 576
Cdd:PRK10946 412 CSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPAVIHAALVSMEDELMGEKSCAFLVVKEPLKAV-QLR 490
|
570 580 590 600
....*....|....*....|....*....|....*....|....*...
gi 15596084 577 QELkqwvrREIGpIAT---PDVIQWAPGLPKTRSGKIMRRILRKIAAA 621
Cdd:PRK10946 491 RFL-----REQG-IAEfklPDRVECVDSLPLTAVGKVDKKQLRQWLAS 532
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
84-581 |
2.99e-16 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 82.25 E-value: 2.99e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHL----AERGDQVAII----WEGDDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRI 155
Cdd:PRK09274 10 RHLpraaQERPDQLAVAvpggRGADGKLAYDELSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 156 GAIHSVVFGGFSPEALAGRIIDCKSKVVITadegvrggkrTPlKANVDDALTNPETSSVQKIIVCKRTGAeikWNQHRdv 235
Cdd:PRK09274 90 GAVPVLVDPGMGIKNLKQCLAEAQPDAFIG----------IP-KAHLARRLFGWGKPSVRRLVTVGGRLL---WGGTT-- 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 236 wYDDLMKV-AGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLvyASLTHER-VFDYRPGEVywctaDIgwvtgH 313
Cdd:PRK09274 154 -LATLLRDgAAAPFPMADLAPDDMAAILFTSGSTGTPKGVVYTHGMFE--AQIEALReDYGIEPGEI-----DL-----P 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 314 TYIVYG--PLANGATTIlfegVPNYpDVTRVAKI--------IDKHKVNILYTAPTAIRAMMAEGKAavAGADGSSLRLL 383
Cdd:PRK09274 221 TFPLFAlfGPALGMTSV----IPDM-DPTRPATVdpaklfaaIERYGVTNLFGSPALLERLGRYGEA--NGIKLPSLRRV 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 384 GSVGEPINPEAWQWYYE---------------------TVGqSRCPIVDTWWQTETGA--CLMTPLPGAHAmkpgsaakp 440
Cdd:PRK09274 294 ISAGAPVPIAVIERFRAmlppdaeiltpygatealpisSIE-SREILFATRAATDNGAgiCVGRPVDGVEV--------- 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 441 ffgVVPALVDNLGNLIEGA---AEGNL-VILDSWPGQARTLFGD---------HDRFVDTYFKtfkgmyfTGDGARRDED 507
Cdd:PRK09274 364 ---RIIAISDAPIPEWDDAlrlATGEIgEIVVAGPMVTRSYYNRpeatrlakiPDGQGDVWHR-------MGDLGYLDAQ 433
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15596084 508 GYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGmqHDIKGQGIYVY-VTLNSGVEPS-EALRQELKQ 581
Cdd:PRK09274 434 GRLWFCGRKAHRVETAGGTLYTIPCERIFNTHPGVKRSALVG--VGVPGAQRPVLcVELEPGVACSkSALYQELRA 507
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
108-619 |
3.11e-16 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 82.18 E-value: 3.11e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVI-- 184
Cdd:PRK12492 50 LSYAELERHSAAFAAYLQQHtDLVPGDRIAVQMPNVLQYPIAVFGALRAGLIVVNTNPLYTAREMRHQFKDSGARALVyl 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 185 -------------TADEGVRGGKRTPLKANVDDALTNPETSSVQKIIVCKRTGAEIKWNQHrdvwyddLMKVAGSTCAPK 251
Cdd:PRK12492 130 nmfgklvqevlpdTGIEYLIEAKMGDLLPAAKGWLVNTVVDKVKKMVPAYHLPQAVPFKQA-------LRQGRGLSLKPV 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 252 EMGAEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLthervfdyrpgEVYWCTADIGwVTGHT---------------YI 316
Cdd:PRK12492 203 PVGLDDIAVLQYTGGTTGLAKGAMLTHGN-LVANML-----------QVRACLSQLG-PDGQPlmkegqevmiaplplYH 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 317 VYGPLAN-------GATTILfegVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEP 389
Cdd:PRK12492 270 IYAFTANcmcmmvsGNHNVL---ITNPRDIPGFIKELGKWRFSALLGLNTLFVALMDH--PGFKDLDFSALKLTNSGGTA 344
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 390 I---NPEAWQwyyetvGQSRCPIVDTWWQTETGACLMTPLPGAHAmKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNL-- 464
Cdd:PRK12492 345 LvkaTAERWE------QLTGCTIVEGYGLTETSPVASTNPYGELA-RLGTVGIPVPGTALKVIDDDGNELPLGERGELci 417
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 465 ----VILDSWPGQARTlfgdhDRFVDTyfktfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHP 540
Cdd:PRK12492 418 kgpqVMKGYWQQPEAT-----AEALDA-----EGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHP 487
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15596084 541 KVAEAAVVGMQHDIKGQGIYVYVTLNsgvEPSEALrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIA 619
Cdd:PRK12492 488 KVANCAAIGVPDERSGEAVKLFVVAR---DPGLSV-EELKAYCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRDIA 562
|
|
| LC_FACS_bac1 |
cd17641 |
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial ... |
106-549 |
1.40e-15 |
|
bacterial long-chain fatty acid CoA synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341296 [Multi-domain] Cd Length: 569 Bit Score: 80.16 E-value: 1.40e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd17641 10 QEFTWADYADRVRAFALGLLALGVGRGDVVAILGDNRPEWVWAELAAQAIGALSLGIYQDSMAEEVAYLLNYTGARVVIA 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 ADEgvrggkrtplkANVDDALT-NPETSSVQKIIVCKRTGAEiKWNQHRDVWYDDLMKvAGSTCAPKEMGA--------- 255
Cdd:cd17641 90 EDE-----------EQVDKLLEiADRIPSVRYVIYCDPRGMR-KYDDPRLISFEDVVA-LGRALDRRDPGLyerevaagk 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 256 -EDPLFILYTSGSTGKPKGVLHTTGGYLVYaSLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANG--------AT 326
Cdd:cd17641 157 gEDVAVLCTTSGTTGKPKLAMLSHGNFLGH-CAAYLAADPLGPGDEYVSVLPLPWIGEQMYSVGQALVCGfivnfpeePE 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 327 TI---LFEGVPNY----P---------------DVTRVAKIIDKHKVNILYTA----------PTAIRAMMAEGKAAVAG 374
Cdd:cd17641 236 TMmedLREIGPTFvllpPrvwegiaadvrarmmDATPFKRFMFELGMKLGLRAldrgkrgrpvSLWLRLASWLADALLFR 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 375 A-----DGSSLRLLGSVGEPINPEAWQwYYETVGqsrCPIVDTWWQTET-GACLMTPlpgAHAMKPGSAAKPFFGvVPAL 448
Cdd:cd17641 316 PlrdrlGFSRLRSAATGGAALGPDTFR-FFHAIG---VPLKQLYGQTELaGAYTVHR---DGDVDPDTVGVPFPG-TEVR 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 449 VDNLGNLIegaAEGNLVILDSWPGQARTlfgDHDRFVDTYFKtfkgmyfTGDGARRDEDGYYWITGRVDDVLNVS-GHRM 527
Cdd:cd17641 388 IDEVGEIL---VRSPGVFVGYYKNPEAT---AEDFDEDGWLH-------TGDAGYFKENGHLVVIDRAKDVGTTSdGTRF 454
|
490 500
....*....|....*....|..
gi 15596084 528 GTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd17641 455 SPQFIENKLKFSPYIAEAVVLG 476
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
88-617 |
6.15e-15 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 78.10 E-value: 6.15e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 88 ERGDQVAIIwegdDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAihsvVFGGFS 167
Cdd:PLN02330 40 LYADKVAFV----EAVTGKAVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGG----VFSGAN 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 168 PEALAGRIidckSKVVITADEgvrggkrtplKANVDDALTNPETSSVQ-KIIVCKRTGAE--IKWNqhrdvwydDLMKVA 244
Cdd:PLN02330 112 PTALESEI----KKQAEAAGA----------KLIVTNDTNYGKVKGLGlPVIVLGEEKIEgaVNWK--------ELLEAA 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 245 ---GSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLvyASLThERVFDYRPgEVYWCTADIGWVTG-HTYivygp 320
Cdd:PLN02330 170 draGDTSDNEEILQTDLCALPFSSGTTGISKGVMLTHRNLV--ANLC-SSLFSVGP-EMIGQVVTLGLIPFfHIY----- 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 321 lanGATTILFEGVPNYPDVTRVAKI--------IDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRL--LGSVGEPI 390
Cdd:PLN02330 241 ---GITGICCATLRNKGKVVVMSRFelrtflnaLITQEVSFAPIVPPIILNLVKN--PIVEEFDLSKLKLqaIMTAAAPL 315
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 391 NPEAWqwyyeTVGQSRCPIV---DTWWQTETGACLMTPlpGAHAMKPGSAAKPFFG-VVPAL----VD-NLGNLIEGAAE 461
Cdd:PLN02330 316 APELL-----TAFEAKFPGVqvqEAYGLTEHSCITLTH--GDPEKGHGIAKKNSVGfILPNLevkfIDpDTGRSLPKNTP 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 462 GNLVILDSWPGQARTLFGDH-DRFVDTyfktfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHP 540
Cdd:PLN02330 389 GELCVRSQCVMQGYYNNKEEtDRTIDE-----DGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHP 463
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 541 KVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PLN02330 464 SVEDAAVVPLPDEEAGEIPAACVVINPKAKESE---EDILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
104-615 |
9.58e-15 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 77.34 E-value: 9.58e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:PRK13383 57 DDGALSYRELQRATESLARRLTRDGVAPGRAVGVMCRNGRGFVTAVFAVGLLGADVVPISTEFRSDALAAALRAHHISTV 136
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITADEGVRGgkrtplKANVDDALTNPETSSVqkiivckrtGAEikwnqhrdvwyddlmkvagsTCAPKEMGAEDPLFILY 263
Cdd:PRK13383 137 VADNEFAER------IAGADDAVAVIDPATA---------GAE--------------------ESGGRPAVAAPGRIVLL 181
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVlhttggylvyaslthERVFDYRPGEVYWCT--ADIGWVTGHTYIVYGPLANG------ATTILFEGV-- 333
Cdd:PRK13383 182 TSGTTGKPKGV---------------PRAPQLRSAVGVWVTilDRTRLRTGSRISVAMPMFHGlglgmlMLTIALGGTvl 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 334 -PNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDT 412
Cdd:PRK13383 247 tHRHFDAEAALAQASLHRADAFTAVPVVLARILELPPRVRARNPLPQLRVVMSSGDRLDPTLGQRFMDTYGDI---LYNG 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 413 WWQTETG-ACLMTPLPGAHAmkPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDSWPGqartlfgdhDRFVDTYFKT 491
Cdd:PRK13383 324 YGSTEVGiGALATPADLRDA--PETVGKPVAGCPVRILDRNNRPVGPRVTGRIFVGGELAG---------TRYTDGGGKA 392
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 492 -FKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVE 570
Cdd:PRK13383 393 vVDGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENALAAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSG 472
|
490 500 510 520
....*....|....*....|....*....|....*....|....*.
gi 15596084 571 -PSEALRQELKQWVRReigpIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK13383 473 vDAAQLRDYLKDRVSR----FEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
107-612 |
1.26e-14 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 76.71 E-value: 1.26e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 107 EITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITA 186
Cdd:cd05914 7 PLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKAIFVS 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 DEgvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyDDLMkvagstcapkemgaedplFILYTSG 266
Cdd:cd05914 87 DE-------------------------------------------------DDVA------------------LINYTSG 99
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 267 STGKPKGVLhttggylvyasLTHE----------RVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPny 336
Cdd:cd05914 100 TTGNSKGVM-----------LTYRnivsnvdgvkEVVLLGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIP-- 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 337 PD---------------VTRVAKIIDKHKVNI------------LYTAPTAIRAMMAEGKaAVAGADGSSLRLLGSVGEP 389
Cdd:cd05914 167 SAkiialafaqvtptlgVPVPLVIEKIFKMDIipkltlkkfkfkLAKKINNRKIRKLAFK-KVHEAFGGNIKEFVIGGAK 245
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 390 INPEAWQWYYeTVGqsrCPIVDTWWQTETGACLMTPLPGahAMKPGSAAKPFFGVVPALVDNLGNLIEGA--AEGNLVIL 467
Cdd:cd05914 246 INPDVEEFLR-TIG---FPYTIGYGMTETAPIISYSPPN--RIRLGSAGKVIDGVEVRIDSPDPATGEGEiiVRGPNVMK 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 468 DSW--PGQARTLFgdhdrfvdtyfkTFKGMYFTGDGARRDEDGYYWITGRVDDV-LNVSGHRMGTAEVESAMVAHPKVAE 544
Cdd:cd05914 320 GYYknPEATAEAF------------DKDGWFHTGDLGKIDAEGYLYIRGRKKEMiVLSSGKNIYPEEIEAKINNMPFVLE 387
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15596084 545 AAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGpIATP------DVIQWAPGLPKTRSGKIMR 612
Cdd:cd05914 388 SLVVVQEKKLVALAYIDPDFLDVKALKQRNIIDAIKWEVRDKVN-QKVPnykkisKVKIVKEEFEKTPKGKIKR 460
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
108-623 |
1.29e-14 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 76.84 E-value: 1.29e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITAD 187
Cdd:PRK07514 29 YTYGDLDAASARLANLLVALGVKPGDRVAVQVEKSPEALALYLATLRAGAVFLPLNTAYTLAELDYFIGDAEPALVVCDP 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 188 EGVRGGKRTPLKANVDDALTNPEtssvqkiivcKRTGAEIkwnqhrdvwydDLMKVAGSTCAPKEMGAEDPLFILYTSGS 267
Cdd:PRK07514 109 ANFAWLSKIAAAAGAPHVETLDA----------DGTGSLL-----------EAAAAAPDDFETVPRGADDLAAILYTSGT 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 268 TGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFegvPNYpDVTRVAKIID 347
Cdd:PRK07514 168 TGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDVLIHALPIFHTHGLFVATNVALLAGASMIFL---PKF-DPDAVLALMP 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 348 KHKVniLYTAPTAIRAMMAE---GKAAVAGadgssLRLLGSVGEPINPEAWQWYYETVGQsrcPIVDTWWQTETGACLMT 424
Cdd:PRK07514 243 RATV--MMGVPTFYTRLLQEprlTREAAAH-----MRLFISGSAPLLAETHREFQERTGH---AILERYGMTETNMNTSN 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 425 PLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAE-GNL------VILDSW--PGQARTLFGDhdrfvDTYFKTfkgm 495
Cdd:PRK07514 313 PYDGER--RAGTVGFPLPGVSLRVTDPETGAELPPGEiGMIevkgpnVFKGYWrmPEKTAEEFRA-----DGFFIT---- 381
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 496 yftGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSE-A 574
Cdd:PRK07514 382 ---GDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVVESAVIGVPHPDFGEGVTAVVVPKPGAALDEaA 458
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 15596084 575 LRQELKqwvrreiGPIA---TPDVIQWAPGLPKTRSGKIMRRILRKIAAAEY 623
Cdd:PRK07514 459 ILAALK-------GRLArfkQPKRVFFVDELPRNTMGKVQKNLLREQYADLF 503
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
263-616 |
1.59e-14 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 76.67 E-value: 1.59e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 263 YTSGSTGKPKG--------VLHTTGGYL--VYASLTHERVFDYRP-------GEVYWCTadigwVTGHTYIVYGPLANGA 325
Cdd:PRK07008 183 YTSGTTGNPKGalyshrstVLHAYGAALpdAMGLSARDAVLPVVPmfhvnawGLPYSAP-----LTGAKLVLPGPDLDGK 257
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 326 TtiLFEgvpnypdvtrvakIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLR---LLGSVGEPINPEAWQWYYETv 402
Cdd:PRK07008 258 S--LYE-------------LIEAERVTFSAGVPTVWLGLLNHMREA--GLRFSTLRrtvIGGSACPPAMIRTFEDEYGV- 319
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 403 gqsrcPIVDTWWQTEtgaclMTP------LPGAHAMKPGSA--------AKPFFGVVPALVDNLGNLI--EGAAEGNLVI 466
Cdd:PRK07008 320 -----EVIHAWGMTE-----MSPlgtlckLKWKHSQLPLDEqrkllekqGRVIYGVDMKIVGDDGRELpwDGKAFGDLQV 389
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 467 LDSWpgqartlfgdhdrFVDTYFKT-----FKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPK 541
Cdd:PRK07008 390 RGPW-------------VIDRYFRGdasplVDGWFPTGDVATIDADGFMQITDRSKDVIKSGGEWISSIDIENVAVAHPA 456
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 542 VAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSealRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILR 616
Cdd:PRK07008 457 VAEAACIACAHPKWDERPLLVVVKRPGAEVT---REELLAFYEGKVAKWWIPDDVVFVDAIPHTATGKLQKLKLR 528
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
79-618 |
1.69e-14 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 76.41 E-value: 1.69e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 79 HNCLDRHlAERGDQVAIIwegdDPADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:cd17642 21 HKAMKRY-ASVPGTIAFT----DAHTGVNYSYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVG 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSPEALAGRIIDCKSKVVITAdegvrggKRTPLK-ANVDDALTnpetsSVQKIIV-----------CKRTGAE 226
Cdd:cd17642 96 VAPTNDIYNERELDHSLNISKPTIVFCS-------KKGLQKvLNVQKKLK-----IIKTIIIldskedykgyqCLYTFIT 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 227 ikwnQHRDVWYDDLMKVAGStcAPKEmgaEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWCTAD 306
Cdd:cd17642 164 ----QNLPPGFNEYDFKPPS--FDRD---EQVALIMNSSGSTGLPKGVQLTHKNIVARFSHARDPIFGNQIIPDTAILTV 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 307 IGWVTGH-TYIVYGPLANGATTILfegVPNYPDVTRVaKIIDKHKVNILYTAPTaIRAMMAEgKAAVAGADGSSLRLLGS 385
Cdd:cd17642 235 IPFHHGFgMFTTLGYLICGFRVVL---MYKFEEELFL-RSLQDYKVQSALLVPT-LFAFFAK-STLVDKYDLSNLHEIAS 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 386 VGEPINPEAWQWYYETVGqsrCPIV-DTWWQTE-TGACLMTPlpgAHAMKPGSAAK--PFFGVvpALVD-NLGNLIEGAA 460
Cdd:cd17642 309 GGAPLSKEVGEAVAKRFK---LPGIrQGYGLTEtTSAILITP---EGDDKPGAVGKvvPFFYA--KVVDlDTGKTLGPNE 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 461 EGNLVIldSWPGQARTLFGDHDRFVDTYFKtfKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHP 540
Cdd:cd17642 381 RGELCV--KGPMIMKGYVNNPEATKALIDK--DGWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHP 456
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 541 KVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEalrQELKQWVRREIGPiatpdvIQWAPG-------LPKTRSGKIMRR 613
Cdd:cd17642 457 KIFDAGVAGIPDEDAGELPAAVVVLEAGKTMTE---KEVMDYVASQVST------AKRLRGgvkfvdeVPKGLTGKIDRR 527
|
....*
gi 15596084 614 ILRKI 618
Cdd:cd17642 528 KIREI 532
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
264-617 |
2.29e-14 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 75.08 E-value: 2.29e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHTTGGYLVYASLTHERVfdyrPGEVYWCTADIGW-VTGHTYIVYGPLANGATTILfeGVPNYPDVTRV 342
Cdd:PRK07824 43 TSGTTGTPKGAMLTAAALTASADATHDRL----GGPGQWLLALPAHhIAGLQVLVRSVIAGSEPVEL--DVSAGFDPTAL 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 343 AKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGAdgssLRLLGSV---GEPINPEAwqwyYETVGQSRCPIVDTWWQTET- 418
Cdd:PRK07824 117 PRAVAELGGGRRYTSLVPMQLAKALDDPAATAA----LAELDAVlvgGGPAPAPV----LDAAAAAGINVVRTYGMSETs 188
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 419 GACLM--TPLPGAHAMkpgsaakpffgvvpalVDNLGNLIEGA--AEG--NLVildswpgqartlfgDHDRFVDtyfktf 492
Cdd:PRK07824 189 GGCVYdgVPLDGVRVR----------------VEDGRIALGGPtlAKGyrNPV--------------DPDPFAE------ 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 493 KGMYFTGDGARRDeDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGvePS 572
Cdd:PRK07824 233 PGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAAVVGDGG--PA 309
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 15596084 573 EALRqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:PRK07824 310 PTLE-ALRAHVARTLDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
106-549 |
2.55e-14 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 75.97 E-value: 2.55e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05932 5 VEFTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRYVLEHSESKALFV 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 adegvrgGKrtpLKANVDDALTNPEtssvqKIIVCKRTGAEIKWNQHRdvwYDDLMKVAGSTCAPKEMGAEDPLFILYTS 265
Cdd:cd05932 85 -------GK---LDDWKAMAPGVPE-----GLISISLPPPSAANCQYQ---WDDLIAQHPPLEERPTRFPEQLATLIYTS 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLHTTGGYLVYASLTHERvFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTILFEGVPNYP-DVTRV-- 342
Cdd:cd05932 147 GTTGQPKGVMLTFGSFAWAAQAGIEH-IGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAESLDTFVeDVQRArp 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 343 --------------AKIIDK---HKVNILYTAPTaIRAMMAEGKAAVAGADgsSLRLLGSVGEPINPEAWQWyYETVGqs 405
Cdd:cd05932 226 tlffsvprlwtkfqQGVQDKipqQKLNLLLKIPV-VNSLVKRKVLKGLGLD--QCRLAGCGSAPVPPALLEW-YRSLG-- 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 406 rCPIVDTWWQTETGACLMTPLPGAHamKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVILDswPGQARTLFGDhdrfv 485
Cdd:cd05932 300 -LNILEAYGMTENFAYSHLNYPGRD--KIGTVGNAGPGVEVRISEDGEILVRSPALMMGYYKD--PEATAEAFTA----- 369
|
410 420 430 440 450 460
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 486 dtyfktfKGMYFTGDGARRDEDGYYWITGRVDDVLNVS-GHRMGTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd05932 370 -------DGFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIG 427
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
87-618 |
3.00e-14 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 75.78 E-value: 3.00e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 87 AERGDQVAIIWEGDDPADhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTrigaihsvvfggf 166
Cdd:cd05906 20 AERGPTKGITYIDADGSE-EFQSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACV------------- 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 167 speaLAGrIIDCKSKVVITADEGVRGGKRtpLKaNVDDALTNPetssvqKIIVCKRTGAEI----KWNQHRD---VWYDD 239
Cdd:cd05906 86 ----LAG-FVPAPLTVPPTYDEPNARLRK--LR-HIWQLLGSP------VVLTDAELVAEFagleTLSGLPGirvLSIEE 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 240 LMKVAGSTCAPkEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVyASLTHERVFDYRPGEVY--WCTADigWVTGHTYIV 317
Cdd:cd05906 152 LLDTAADHDLP-QSRPDDLALLMLTSGSTGFPKAVPLTHRNILA-RSAGKIQHNGLTPQDVFlnWVPLD--HVGGLVELH 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 YGPLANGATTIlfegvpNYP------DVTRVAKIIDKHKVNILYtAPTAIRAMMAEGKAAVAGADG--SSLRLLGSVGEP 389
Cdd:cd05906 228 LRAVYLGCQQV------HVPteeilaDPLRWLDLIDRYRVTITW-APNFAFALLNDLLEEIEDGTWdlSSLRYLVNAGEA 300
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 390 INPEAWQWYYETVGQSRCP---IVDTWWQTETGA-------CLMTPLPGAHAMKpgSAAKPFFGVVPALVDNLGNLIEGA 459
Cdd:cd05906 301 VVAKTIRRLLRLLEPYGLPpdaIRPAFGMTETCSgviysrsFPTYDHSQALEFV--SLGRPIPGVSMRIVDDEGQLLPEG 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 460 AEGNLVIldswpgQARTLFgdhdrfvDTYFK---------TFKGMYFTGDGARRDeDGYYWITGRVDDVLNVSGHRMGTA 530
Cdd:cd05906 379 EVGRLQV------RGPVVT-------KGYYNnpeanaeafTEDGWFRTGDLGFLD-NGNLTITGRTKDTIIVNGVNYYSH 444
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 531 EVESAM-----VAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQeLKQWVRREIGpIATPDVIQWAPG-LPK 604
Cdd:cd05906 445 EIEAAVeevpgVEPSFTAAFAVRDPGAETEELAIFFVPEYDLQDALSETLRA-IRSVVSREVG-VSPAYLIPLPKEeIPK 522
|
570
....*....|....
gi 15596084 605 TRSGKIMRRILRKI 618
Cdd:cd05906 523 TSLGKIQRSKLKAA 536
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
90-641 |
1.12e-13 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 74.05 E-value: 1.12e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 90 GDQVAIIWEGDDPadhQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIhsvvfggFSP 168
Cdd:PRK05620 24 GDTTVTTWGGAEQ---EQTTFAAIGARAAALAHALHDElGITGDQRVGSMMYNCAEHLEVLFAVACMGAV-------FNP 93
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 169 --EALAG----RIIDCKSKVVITADEGVRGGKRTPLkanvddaltnPETSSVQKIIVCKRTGAEIKWNQ---HRDVW-YD 238
Cdd:PRK05620 94 lnKQLMNdqivHIINHAEDEVIVADPRLAEQLGEIL----------KECPCVRAVVFIGPSDADSAAAHmpeGIKVYsYE 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 239 DLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGG-YLVYASLTHERVFDYRPGEVYWCTADIGWVtghtyIV 317
Cdd:PRK05620 164 ALLDGRSTVYDWPELDETTAAAICYSTGTTGAPKGVVYSHRSlYLQSLSLRTTDSLAVTHGESFLCCVPIYHV-----LS 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 YG-PLAN--GATTILFEGvpnyPDVT--RVAKIIDKHKVNILYTAPTAIRAMMAEgkAAVAGADGSSLRLLGSVGEPINP 392
Cdd:PRK05620 239 WGvPLAAfmSGTPLVFPG----PDLSapTLAKIIATAMPRVAHGVPTLWIQLMVH--YLKNPPERMSLQEIYVGGSAVPP 312
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 393 ---EAWQWYYETvgqsrcPIVDTWWQTETGACLMTPLPGAHAmkPGSAAKPFF---GVVPA----LVDNLGNLIEGA--A 460
Cdd:PRK05620 313 iliKAWEERYGV------DVVHVWGMTETSPVGTVARPPSGV--SGEARWAYRvsqGRFPAsleyRIVNDGQVMESTdrN 384
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 461 EGNLVILDSW-------------PGQARTLFGDHDRFVDTYFkTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRM 527
Cdd:PRK05620 385 EGEIQVRGNWvtasyyhspteegGGAASTFRGEDVEDANDRF-TADGWLRTGDVGSVTRDGFLTIHDRARDVIRSGGEWI 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 528 GTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRS 607
Cdd:PRK05620 464 YSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLRDQLRDRLPNWMLPEYWTFVDEIDKTSV 543
|
570 580 590
....*....|....*....|....*....|....*
gi 15596084 608 GKIMRRILRK-IAAAEYdtlgDISTLADPGVVQHL 641
Cdd:PRK05620 544 GKFDKKDLRQhLADGDF----EIIKLKGPGESGES 574
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
105-617 |
2.68e-13 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 72.46 E-value: 2.68e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 105 HQEITYRQLHEQVCKFANALRGQ-DVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd05937 3 GKTWTYSETYDLVLRYAHWLHDDlGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRFV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ItadegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwYDDlmkvagstcapkemgaEDPLFILY 263
Cdd:cd05937 83 I----------------------------------------------------VDP----------------DDPAILIY 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPGEVYWC------TADIgwvTGHTYIvygpLANGATTIL---FEGVP 334
Cdd:cd05937 95 TSGTTGLPKAAAISWRRTLVTSNLLSHDLNLKNGDRTYTCmplyhgTAAF---LGACNC----LMSGGTLALsrkFSASQ 167
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 335 NYPDVtrvakiIDKHKVNILYTAPTaIRAMMA------EGKAAVAGADGSSLRllgsvgepinPEAWQWYYETVGqsrCP 408
Cdd:cd05937 168 FWKDV------RDSGATIIQYVGEL-CRYLLStppspyDRDHKVRVAWGNGLR----------PDIWERFRERFN---VP 227
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 409 IVDTWWQTETGACLMTPLP----GAHAMK-PGSAAKPFFG--VVPALVD-NLGNLIEGAAEGNLV---------ILDSWP 471
Cdd:cd05937 228 EIGEFYAATEGVFALTNHNvgdfGAGAIGhHGLIRRWKFEnqVVLVKMDpETDDPIRDPKTGFCVrapvgepgeMLGRVP 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 472 GQARTLFGDH--------DRFVDTYFKtfKG-MYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPK 541
Cdd:cd05937 308 FKNREAFQGYlhnedateSKLVRDVFR--KGdIYFrTGDLLRQDADGRWYFLDRLGDTFRWKSENVSTTEVADVLGAHPD 385
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 542 VAEAAVVGMQ---HDikGQGIYVYVTL-NSGVEPSEALRQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05937 386 IAEANVYGVKvpgHD--GRAGCAAITLeESSAVPTEFTKSLLASLARKNLPSYAVPLFLRLTEEVATTDNHKQQKGVLRD 463
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
497-615 |
3.89e-12 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 68.52 E-value: 3.89e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 497 FTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSealr 576
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVISHEEIDPV---- 369
|
90 100 110
....*....|....*....|....*....|....*....
gi 15596084 577 qELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:PRK08308 370 -QLREWCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL 407
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
255-585 |
4.87e-12 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 68.64 E-value: 4.87e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 255 AEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERvFDYRPGEVYWCTADIgwvtghtYIVYGPlANGATTILFEGVP 334
Cdd:cd05910 84 ADEPAAILFTSGSTGTPKGVVYRHGTFAAQIDALRQL-YGIRPGEVDLATFPL-------FALFGP-ALGLTSVIPDMDP 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 335 NYPDVTRVAKIID---KHKVNILYTAPTAIRAMMAEGkaAVAGADGSSLRLLGSVGEPINPEAWQWYYETV--------- 402
Cdd:cd05910 155 TRPARADPQKLVGairQYGVSIVFGSPALLERVARYC--AQHGITLPSLRRVLSAGAPVPIALAARLRKMLsdeaeiltp 232
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 403 -GQSRC-PI---------VDTWWQTETGA--CLMTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVIlDS 469
Cdd:cd05910 233 yGATEAlPVssigsrellATTTAATSGGAgtCVGRPIPGVRVRIIEIDDEPIAEWDDTLELPRGEIGEITVTGPTVT-PT 311
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 470 WPGQArtlfgDHDRF--VDTYFKTFkgMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAV 547
Cdd:cd05910 312 YVNRP-----VATALakIDDNSEGF--WHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTHPGVRRSAL 384
|
330 340 350
....*....|....*....|....*....|....*...
gi 15596084 548 VGMQHDIKGQGIYVYVTLNSGVEPSEALRQELKQWVRR 585
Cdd:cd05910 385 VGVGKPGCQLPVLCVEPLPGTITPRARLEQELRALAKD 422
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
260-615 |
5.98e-12 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 68.20 E-value: 5.98e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 260 FILYTSGSTGKPKGVLHTTGGYLVYASLTHERVFDYRPG-EVYWCTAD-----------IGWVTGHTYIVYGPLANGatt 327
Cdd:cd17648 98 YAIYTSGTTGKPKGVLVEHGSVVNLRTSLSERYFGRDNGdEAVLFFSNyvfdffveqmtLALLNGQKLVVPPDEMRF--- 174
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 328 ilfegvpnypDVTRVAKIIDKHKVNILYTAPTAIRaMMAEGKAavagadgSSLRLLGSVGEPINPeawQWYYETVGQSRC 407
Cdd:cd17648 175 ----------DPDRFYAYINREKVTYLSGTPSVLQ-QYDLARL-------PHLKRVDAAGEEFTA---PVFEKLRSRFAG 233
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 408 PIVDTWWQTETGA---------------CLMTPLPGAHAMKPGSAAKPF-FGVVPALVdnLGNLieGAAEGNLvildSWP 471
Cdd:cd17648 234 LIINAYGPTETTVtnhkrffpgdqrfdkSLGRPVRNTKCYVLNDAMKRVpVGAVGELY--LGGD--GVARGYL----NRP 305
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 472 GQARtlfgdhDRFVDTYFKT--------FKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVA 543
Cdd:cd17648 306 ELTA------ERFLPNPFQTeqerargrNARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVR 379
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15596084 544 EAAVVGMQHDIKGQG-----IYVYVTLNSGVEPSealrQELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRIL 615
Cdd:cd17648 380 ECAVVAKEDASQAQSriqkyLVGYYLPEPGHVPE----SDLLSFLRAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK08633 |
PRK08633 |
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated |
255-621 |
2.29e-11 |
|
2-acyl-glycerophospho-ethanolamine acyltransferase; Validated
Pssm-ID: 236315 [Multi-domain] Cd Length: 1146 Bit Score: 67.26 E-value: 2.29e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 255 AEDPLFILYTSGSTGKPKGVLhttggylvyasLTHER----------VFDYRPGEVYWCTADIGWVTGHTYIVYGPLANG 324
Cdd:PRK08633 781 PDDTATIIFSSGSEGEPKGVM-----------LSHHNilsnieqisdVFNLRNDDVILSSLPFFHSFGLTVTLWLPLLEG 849
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 325 ATTILfegVPNYPDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKaaVAGADGSSLRLLGSVGEPINPEAWQWYYETVGQ 404
Cdd:PRK08633 850 IKVVY---HPDPTDALGIAKLVAKHRATILLGTPTFLRLYLRNKK--LHPLMFASLRLVVAGAEKLKPEVADAFEEKFGI 924
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 405 srcPIVDTWWQTETG--ACLMTP---LPGA---HAMKPGSAAKPFFGVVPALVD--NLGNLIEGAaEGNLVIldSWPGQA 474
Cdd:PRK08633 925 ---RILEGYGATETSpvASVNLPdvlAADFkrqTGSKEGSVGMPLPGVAVRIVDpeTFEELPPGE-DGLILI--GGPQVM 998
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFGDHDRfVDTYFKTFKGM--YFTGDGARRDEDGYYWITGRVDDVLNVSGHrmgtaevesaMVAHPKVAEA------- 545
Cdd:PRK08633 999 KGYLGDPEK-TAEVIKDIDGIgwYVTGDKGHLDEDGFLTITDRYSRFAKIGGE----------MVPLGAVEEElakalgg 1067
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 546 -----AVVGMQHDIKGQGIYVYVTLNSgvEPSEALRQELKQwvrREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKIAA 620
Cdd:PRK08633 1068 eevvfAVTAVPDEKKGEKLVVLHTCGA--EDVEELKRAIKE---SGLPNLWKPSRYFKVEALPLLGSGKLDLKGLKELAL 1142
|
.
gi 15596084 621 A 621
Cdd:PRK08633 1143 A 1143
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
257-608 |
7.11e-11 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 63.86 E-value: 7.11e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 257 DPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVYWctadigwVTGHTYIVyGPLANGATTILFEG---- 332
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQA-LLAQALVLAVLQAIDEGTVFL-------NSGPLFHI-GTLMFTLATFHAGGtnvf 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 333 VPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAvaGADGSSLRLLgsvgepinPEAWQWyyetvgQSRCPIVDT 412
Cdd:cd17636 72 VRRV-DAEEVLELIEAERCTHAFLLPPTIDQIVELNADG--LYDLSSLRSS--------PAAPEW------NDMATVDTS 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 413 WW--------QTETGACLMTPLPGAHAMkpGSAAKPFFGVVPALVDNLGNLI------EGAAEGNLVILDSW--PG--QA 474
Cdd:cd17636 135 PWgrkpggygQTEVMGLATFAALGGGAI--GGAGRPSPLVQVRILDEDGREVpdgevgEIVARGPTVMAGYWnrPEvnAR 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 475 RTLFGDHdrfvdtyfktfkgmyFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDI 554
Cdd:cd17636 213 RTRGGWH---------------HTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGVPDPR 277
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 15596084 555 KGQGIYVYVTLNSGVEPSEAlrqELKQWVRREIGPIATPDVIQWAPGLPKTRSG 608
Cdd:cd17636 278 WAQSVKAIVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
82-622 |
5.53e-10 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 62.14 E-value: 5.53e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEgddpadHQEI--TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIH 159
Cdd:PRK08315 22 LDRTAARYPDREALVYR------DQGLrwTYREFNEEVDALAKGLLALGIEKGDRVGIWAPNVPEWVLTQFATAKIGAIL 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 160 SVVFGGFSPEAL--AGRIIDCKskVVITADegvrGGKRTPLKANVDDALtnPETSSVQKIIVCKRTGAEIKW------NQ 231
Cdd:PRK08315 96 VTINPAYRLSELeyALNQSGCK--ALIAAD----GFKDSDYVAMLYELA--PELATCEPGQLQSARLPELRRviflgdEK 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 232 HRDVW-YDDLMKVAGSTCAPK--EMGAE----DPLFILYTSGSTGKPKGVLHT-----TGGYLVYAS--LTHErvfDyR- 296
Cdd:PRK08315 168 HPGMLnFDELLALGRAVDDAElaARQATldpdDPINIQYTSGTTGFPKGATLThrnilNNGYFIGEAmkLTEE---D-Rl 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 297 --PGEVYWCtadIGWVTGhtyiVYGPLANGATTIlfegvpnYP----DVTRVAKIIDKHKVNILYTAPTAIRAMMAEgkA 370
Cdd:PRK08315 244 ciPVPLYHC---FGMVLG----NLACVTHGATMV-------YPgegfDPLATLAAVEEERCTALYGVPTMFIAELDH--P 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 371 AVAGADGSSLR---LLGSVgepinpeawqwyyetvgqsrCPI------VD---------TWWQTETGaclmtplPGAHAM 432
Cdd:PRK08315 308 DFARFDLSSLRtgiMAGSP--------------------CPIevmkrvIDkmhmsevtiAYGMTETS-------PVSTQT 360
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 433 KPGSA----------AKPFFGVvpALVDNLGNLI-------EGAAEGNLVILDSWPGQARTLFG-DHDRFvdtyfktfkg 494
Cdd:PRK08315 361 RTDDPlekrvttvgrALPHLEV--KIVDPETGETvprgeqgELCTRGYSVMKGYWNDPEKTAEAiDADGW---------- 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 495 MYfTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIYVYVTLNSGVEPSEa 574
Cdd:PRK08315 429 MH-TGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLTE- 506
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|.
gi 15596084 575 lrQELKQWVRreiGPIA---TPDVIQWAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK08315 507 --EDVRDFCR---GKIAhykIPRYIRFVDEFPMTVTGKIQKFKMREMMIEE 552
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
382-623 |
8.54e-10 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 61.55 E-value: 8.54e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 382 LLGsvGEPinpeAWQWYYETVGQSRCPIVDTWWQTETGACLMTPLPGAHAMKPGSAAKpffgVVP-ALVDnlgnlIEGAA 460
Cdd:PRK07445 236 LLG--GAP----AWPSLLEQARQLQLRLAPTYGMTETASQIATLKPDDFLAGNNSSGQ----VLPhAQIT-----IPANQ 300
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 461 EGNLVIldswpgQARTLF-GDHDRFVDTYfktfkGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAH 539
Cdd:PRK07445 301 TGNITI------QAQSLAlGYYPQILDSQ-----GIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILAT 369
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 540 PKVAEAAVVGMQHDIKGQGIY-VYVTLNSGVEPSealrqELKQWVRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRKI 618
Cdd:PRK07445 370 GLVQDVCVLGLPDPHWGEVVTaIYVPKDPSISLE-----ELKTAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQI 444
|
....*
gi 15596084 619 AAAEY 623
Cdd:PRK07445 445 AVQRL 449
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
106-617 |
2.05e-09 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 60.13 E-value: 2.05e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05939 2 RHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALIF 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 adegvrggkrtplkaNVDDALTNpETSSVQKIIVCKrtgaeikwnqhrdvwyddlmkvagstcapkemGAEDPLFILYTS 265
Cdd:cd05939 82 ---------------NLLDPLLT-QSSTEPPSQDDV--------------------------------NFRDKLFYIYTS 113
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLHTTGGYLVYASLTHeRVFDYRPGEVYWCTADIGWVTGHTYIVYGPLANGATTIL---FEGVPNYPDVtrv 342
Cdd:cd05939 114 GTTGLPKAAVIVHSRYYRIAAGAY-YAFGMRPEDVVYDCLPLYHSAGGIMGVGQALLHGSTVVIrkkFSASNFWDDC--- 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 343 akiiDKHKVNILYTAPTAIRAMMA------EGKAAVAGADGSSLRllgsvgepinPEAWQwyyETVGQSRCP-IVDTWWQ 415
Cdd:cd05939 190 ----VKYNCTIVQYIGEICRYLLAqppseeEQKHNVRLAVGNGLR----------PQIWE---QFVRRFGIPqIGEFYGA 252
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 416 TE-----------TGACLMTPLPGaHAMKPGSAAKpffgvvpalVD-NLGNLIEGAaegNLVILDSWPGQARTLFG---- 479
Cdd:cd05939 253 TEgnsslvnidnhVGACGFNSRIL-PSVYPIRLIK---------VDeDTGELIRDS---DGLCIPCQPGEPGLLVGkiiq 319
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 480 -DHDRFVDTY--------------FKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAE 544
Cdd:cd05939 320 nDPLRRFDGYvnegatnkkiardvFKKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVLGLED 399
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 545 AAVVGMQ--HDIKGQGIYVYVTLNSGVEPsEALRQELkqwvRREIGPIATPDVIQWAPGLPKTRSGKIMRRILRK 617
Cdd:cd05939 400 VVVYGVEvpGVEGRAGMAAIVDPERKVDL-DRFSAVL----AKSLPPYARPQFIRLLPEVDKTGTFKLQKTDLQK 469
|
|
| PLN02430 |
PLN02430 |
long-chain-fatty-acid-CoA ligase |
109-292 |
4.09e-09 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178049 [Multi-domain] Cd Length: 660 Bit Score: 59.83 E-value: 4.09e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 109 TYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALaGRIID-CKSKVVITAD 187
Cdd:PLN02430 78 TYKEVYEEVLQIGSALRASGAEPGSRVGIYGSNCPQWIVAMEACAAHSLICVPLYDTLGPGAV-DYIVDhAEIDFVFVQD 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 188 EGVRGgkrtplkanvddaLTNPETSSVQ--KIIVCKRTGAEIKWNQHRDV------WyDDLMKVA----GSTCAPKemgA 255
Cdd:PLN02430 157 KKIKE-------------LLEPDCKSAKrlKAIVSFTSVTEEESDKASQIgvktysW-IDFLHMGkenpSETNPPK---P 219
|
170 180 190
....*....|....*....|....*....|....*..
gi 15596084 256 EDPLFILYTSGSTGKPKGVLhttggylvyasLTHERV 292
Cdd:PLN02430 220 LDICTIMYTSGTSGDPKGVV-----------LTHEAV 245
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
238-617 |
5.87e-09 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 59.04 E-value: 5.87e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 238 DDLMKVAGSTCAPKEMGA-EDPLFILYTSGSTGKPKGVL--HTTggyLVYASLTHERVFDYRPGEVYWCTADIGWVTGht 314
Cdd:PLN02860 153 EMLKQRALGTTELDYAWApDDAVLICFTSGTTGRPKGVTisHSA---LIVQSLAKIAIVGYGEDDVYLHTAPLCHIGG-- 227
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 315 yiVYGPLAN---GATTILfegVPNYpDVTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGADGSSLRLL----GSVG 387
Cdd:PLN02860 228 --LSSALAMlmvGACHVL---LPKF-DAKAALQAIKQHNVTSMITVPAMMADLISLTRKSMTWKVFPSVRKIlnggGSLS 301
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 388 EPINPEAWQWYyetvgqSRCPIVDTWWQTETGACL-MTPLPGAHAMKPGSAAKPFFGVVPALVDNLGNLIEGAAEGNLVI 466
Cdd:PLN02860 302 SRLLPDAKKLF------PNAKLFSAYGMTEACSSLtFMTLHDPTLESPKQTLQTVNQTKSSSVHQPQGVCVGKPAPHVEL 375
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 467 LDSWPGQART--LF--GDH-------DRFVDTYFKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESA 535
Cdd:PLN02860 376 KIGLDESSRVgrILtrGPHvmlgywgQNSETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAV 455
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 536 MVAHPKVAEAAVVGMQHD-----------IKGQGIYVYVTLNSGVEPSEALRQELKQWVRRE-IGPIATPD-VIQWAPGL 602
Cdd:PLN02860 456 LSQHPGVASVVVVGVPDSrltemvvacvrLRDGWIWSDNEKENAKKNLTLSSETLRHHCREKnLSRFKIPKlFVQWRKPF 535
|
410
....*....|....*
gi 15596084 603 PKTRSGKIMRRILRK 617
Cdd:PLN02860 536 PLTTTGKIRRDEVRR 550
|
|
| AFD_CAR-like |
cd17632 |
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation ... |
108-277 |
1.09e-08 |
|
adenylation domain of carboxylic acid reductase (CAR); This family contains the adenylation domain of carboxylic acid reductase enzymes (CARs), and performs an equivalent function to that of the ANL superfamily of adenylating enzymes. It takes a carboxylic acid substrate and ATP, and produces an AMP-acyl phosphoester intermediate, releasing pyrophosphate. Kinetic analysis using various substrates shows that this enzyme has a broad but similar substrate specificity, preferring electron-rich acids. This suggests that attack by the carboxylate on the alpha-phosphate of adenosine triphosphate (ATP) is the step that determines the substrate specificity and reaction kinetics. CAR is an important enzyme for use as a biocatalyst providing regiospecific route to aldehydes from their respective carboxylic acids.
Pssm-ID: 341287 [Multi-domain] Cd Length: 588 Bit Score: 58.24 E-value: 1.09e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALR-GQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGrIIDCKSKVVITA 186
Cdd:cd17632 68 ITYAELWERVGAVAAAHDpEQPVRPGDFVAVLGFTSPDYATVDLALTRLGAVSVPLQAGASAAQLAP-ILAETEPRLLAV 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 187 D--------EGVRGGkRTPLKANVDDAltNPETSSVQKIIVCKRTGAEikwNQHRDVWYDDLMKVAGSTCAPKEMGAE-- 256
Cdd:cd17632 147 SaehldlavEAVLEG-GTPPRLVVFDH--RPEVDAHRAALESARERLA---AVGIPVTTLTLIAVRGRDLPPAPLFRPep 220
|
170 180
....*....|....*....|....
gi 15596084 257 --DPL-FILYTSGSTGKPKGVLHT 277
Cdd:cd17632 221 ddDPLaLLIYTSGSTGTPKGAMYT 244
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
243-613 |
3.19e-08 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 56.54 E-value: 3.19e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 243 VAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYASLTHERVfDYRPGE---VYW--CTADIGWVTGHTYiv 317
Cdd:PRK07768 139 LAADPIDPVETGEDDLALMQLTSGSTGSPKAVQITHGNLYANAEAMFVAA-EFDVETdvmVSWlpLFHDMGMVGFLTV-- 215
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 318 ygPLANGATTILFEGVPNYPDVTRVAKIIDKHKVNILyTAPTAIRAMMAE--GKAAVAGA-DGSSLRLLGSVGEPINPEA 394
Cdd:PRK07768 216 --PMYFGAELVKVTPMDFLRDPLLWAELISKYRGTMT-AAPNFAYALLARrlRRQAKPGAfDLSSLRFALNGAEPIDPAD 292
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 395 WQWYYET---VGQSRCPIVDTWWQTET---------GACLMTPLPGAHAMKP-GSAAKPFFGVVPALVDnLGNLIEGAaE 461
Cdd:PRK07768 293 VEDLLDAgarFGLRPEAILPAYGMAEAtlavsfspcGAGLVVDEVDADLLAAlRRAVPATKGNTRRLAT-LGPPLPGL-E 370
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 462 GNLVILDSWPGQART-----LFGDH--------DRFVDTyfKTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMG 528
Cdd:PRK07768 371 VRVVDEDGQVLPPRGvgvieLRGESvtpgyltmDGFIPA--QDADGWLDTGDLGYLTEEGEVVVCGRVKDVIIMAGRNIY 448
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 529 TAEVESAMVAHPKV--AEAAVVGMQHDIKGQGIYVYVTLNSGVEPSE--ALRQELKQWVRREIGpIATPDVIQWAPG-LP 603
Cdd:PRK07768 449 PTDIERAAARVEGVrpGNAVAVRLDAGHSREGFAVAVESNAFEDPAEvrRIRHQVAHEVVAEVG-VRPRNVVVLGPGsIP 527
|
410
....*....|
gi 15596084 604 KTRSGKIMRR 613
Cdd:PRK07768 528 KTPSGKLRRA 537
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
91-274 |
4.68e-08 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 56.06 E-value: 4.68e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 91 DQVAIIWEGddpadhQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIG------AIHSvvfg 164
Cdd:PRK04813 17 DFPAYDYLG------EKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAGhayipvDVSS---- 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 165 gfsPEALAGRIID-CKSKVVITADEgvrggkrtplkanvddalTNPETSSVQKIivckrTGAEIKWNQHRDVWYDDLMKV 243
Cdd:PRK04813 87 ---PAERIEMIIEvAKPSLIIATEE------------------LPLEILGIPVI-----TLDELKDIFATGNPYDFDHAV 140
|
170 180 190
....*....|....*....|....*....|.
gi 15596084 244 AGstcapkemgaEDPLFILYTSGSTGKPKGV 274
Cdd:PRK04813 141 KG----------DDNYYIIFTSGTTGKPKGV 161
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
108-330 |
1.52e-07 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 54.60 E-value: 1.52e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVITad 187
Cdd:PTZ00216 122 ITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALAYALRETECKAIVC-- 199
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 188 egvrGGKRTPlkaNVDDALTNPETSSVqKIIVCKRTGAEIKWNQHRDV-WYDDLMK--VAGSTCAPKEMGAEDPL-FILY 263
Cdd:PTZ00216 200 ----NGKNVP---NLLRLMKSGGMPNT-TIIYLDSLPASVDTEGCRLVaWTDVVAKghSAGSHHPLNIPENNDDLaLIMY 271
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 15596084 264 TSGSTGKPKGVLHTTGGYLVYASLTHERVFD----YRPGEvywctadigwvtghTYIVYGPLAN----GATTILF 330
Cdd:PTZ00216 272 TSGTTGDPKGVMHTHGSLTAGILALEDRLNDligpPEEDE--------------TYCSYLPLAHimefGVTNIFL 332
|
|
| PRK09188 |
PRK09188 |
serine/threonine protein kinase; Provisional |
535-648 |
9.75e-07 |
|
serine/threonine protein kinase; Provisional
Pssm-ID: 236400 [Multi-domain] Cd Length: 365 Bit Score: 51.30 E-value: 9.75e-07
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 535 AMVAHPKVAEAAVVGMQHDIKGQGIYVYV-TLNSGVEpsEALRQELKQwvrreIGPIATPDVIQWAPGLPKTRSGKIMRR 613
Cdd:PRK09188 248 ALKSDPAVSDVAIALFSLPAKGVGLYAFVeAELPADE--KSLRARLAG-----AKPPKPPEHIQPVAALPRDADGTVRDD 320
|
90 100 110
....*....|....*....|....*....|....*.
gi 15596084 614 ILRKIAAAEYDTLGD-ISTLADPGVVQHLIETHRSM 648
Cdd:PRK09188 321 ILRLIAMNQIDELDDlLREPEIRGLVEAIAAHRLNL 356
|
|
| PRK12476 |
PRK12476 |
putative fatty-acid--CoA ligase; Provisional |
82-274 |
1.23e-06 |
|
putative fatty-acid--CoA ligase; Provisional
Pssm-ID: 171527 [Multi-domain] Cd Length: 612 Bit Score: 51.67 E-value: 1.23e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVA---IIWEGDDPADHQEITYRQLHEQVCKFANALRgQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAI 158
Cdd:PRK12476 40 IERNIANVGDTVAyryLDHSHSAAGCAVELTWTQLGVRLRAVGARLQ-QVAGPGDRVAILAPQGIDYVAGFFAAIKAGTI 118
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 159 HSVVFGGFSP---EALAGRIIDCKSKVVITAdegvrggkrTPLKANVDDALTNPETSSVQKIIVCkrtgaeikwnqhrdv 235
Cdd:PRK12476 119 AVPLFAPELPghaERLDTALRDAEPTVVLTT---------TAAAEAVEGFLRNLPRLRRPRVIAI--------------- 174
|
170 180 190
....*....|....*....|....*....|....*....
gi 15596084 236 wyDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGV 274
Cdd:PRK12476 175 --DAIPDSAGESFVPVELDTDDVSHLQYTSGSTRPPVGV 211
|
|
| PRK09029 |
PRK09029 |
O-succinylbenzoic acid--CoA ligase; Provisional |
84-285 |
1.46e-06 |
|
O-succinylbenzoic acid--CoA ligase; Provisional
Pssm-ID: 236363 [Multi-domain] Cd Length: 458 Bit Score: 51.03 E-value: 1.46e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 84 RHLAE-RGDQVAIIwegddpADHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAihsvv 162
Cdd:PRK09029 10 RHWAQvRPQAIALR------LNDEVLTWQQLCARIDQLAAGFAQQGVVEGSGVALRGKNSPETLLAYLALLQCGA----- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 163 fggfspealagRIidckskvvitadegvrggkrtplkanvddALTNPETSSVQKIIVCKRTGAEIKWNQHRDVWYDDLMK 242
Cdd:PRK09029 79 -----------RV-----------------------------LPLNPQLPQPLLEELLPSLTLDFALVLEGENTFSALTS 118
|
170 180 190 200
....*....|....*....|....*....|....*....|....*.
gi 15596084 243 V---AGSTCAPKEMGAEDPLFILYTSGSTGKPKGVLHTTGGYLVYA 285
Cdd:PRK09029 119 LhlqLVEGAHAVAWQPQRLATMTLTSGSTGLPKAAVHTAQAHLASA 164
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
104-543 |
1.70e-06 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 51.06 E-value: 1.70e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVV 183
Cdd:cd17639 2 EYKYMSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIHSLNETECSAI 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 184 ITadegvrggkrtplkanvddaltNPEtssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgAEDPLFILY 263
Cdd:cd17639 82 FT----------------------DGK--------------------------------------------PDDLACIMY 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 264 TSGSTGKPKGVLHT-------TGG--------------YLVYASLTH------ERVFDYRPGEvywctadIGWVTGHTY- 315
Cdd:cd17639 96 TSGSTGNPKGVMLThgnlvagIAGlgdrvpellgpddrYLAYLPLAHifelaaENVCLYRGGT-------IGYGSPRTLt 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 316 --IVYGPlaNGATT----ILFEGVPNYPDvtRVAK-IIDK------HKVNILYTAPTAIRAMMAEGK----------AAV 372
Cdd:cd17639 169 dkSKRGC--KGDLTefkpTLMVGVPAIWD--TIRKgVLAKlnpmggLKRTLFWTAYQSKLKALKEGPgtplldelvfKKV 244
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 373 AGADGSSLRLLGSVGEPINPEAwQWYYETVGqsrCPIVDTWWQTETGACLMTPLPGahAMKPGSAAKPFFGVVPALVDnl 452
Cdd:cd17639 245 RAALGGRLRYMLSGGAPLSADT-QEFLNIVL---CPVIQGYGLTETCAGGTVQDPG--DLETGRVGPPLPCCEIKLVD-- 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 453 gnliegAAEGNLvILDSWPGQARTLFGdhDRFVDT-YFK---------TFKGMYFTGDGARRDEDGYYWITGRVDD-VLN 521
Cdd:cd17639 317 ------WEEGGY-STDKPPPRGEILIR--GPNVFKgYYKnpektkeafDGDGWFHTGDIGEFHPDGTLKIIDRKKDlVKL 387
|
490 500
....*....|....*....|..
gi 15596084 522 VSGHRMGTAEVESAMVAHPKVA 543
Cdd:cd17639 388 QNGEYIALEKLESIYRSNPLVN 409
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
106-551 |
1.81e-06 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 50.82 E-value: 1.81e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 106 QEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSKVVIT 185
Cdd:cd05940 2 EALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLVV 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 186 adegvrggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdvwyddlmkvagstcapkemgaeDPLFILYTS 265
Cdd:cd05940 82 -----------------------------------------------------------------------DAALYIYTS 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 266 GSTGKPKGVLHT-----TGGYLVYAS---LTHERVFDYRPgeVYWCTADI-GWVTGhtyivygpLANGATTIL---FEGV 333
Cdd:cd05940 91 GTTGLPKAAIIShrrawRGGAFFAGSggaLPSDVLYTCLP--LYHSTALIvGWSAC--------LASGATLVIrkkFSAS 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 334 PNYPDVTrvakiidKHKVNILYTAPTAIRAMMA------EGKAAVAGADGSSLRllgsvgepinPEAWQWYYETVGQSRc 407
Cdd:cd05940 161 NFWDDIR-------KYQATIFQYIGELCRYLLNqppkptERKHKVRMIFGNGLR----------PDIWEEFKERFGVPR- 222
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 408 pIVDTWWQTEtGACLMTPL---PGAHAMKPGSAAKPF-FGVVPALVDNlGNLIEGAAEGNLVILDSWPGQARTLFGDHDR 483
Cdd:cd05940 223 -IAEFYAATE-GNSGFINFfgkPGAIGRNPSLLRKVApLALVKYDLES-GEPIRDAEGRCIKVPRGEPGLLISRINPLEP 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 484 FvDTYFK-----------TFK--GMYF-TGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVG 549
Cdd:cd05940 300 F-DGYTDpaatekkilrdVFKkgDAWFnTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYG 378
|
..
gi 15596084 550 MQ 551
Cdd:cd05940 379 VQ 380
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
104-277 |
3.06e-06 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 50.29 E-value: 3.06e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 104 DHQEITYRQLHEQVCKFANALR--GQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAGRIIDCKSK 181
Cdd:cd05927 2 PYEWISYKEVAERADNIGSALRslGGKPAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEIS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 182 VVItADEGVRggkrtplkanvddaltnpetssvqkiivckrtgaeikwnqhrdVW-YDDLMKV-AGSTCAPKEMGAEDPL 259
Cdd:cd05927 82 IVF-CDAGVK-------------------------------------------VYsLEEFEKLgKKNKVPPPPPKPEDLA 117
|
170
....*....|....*...
gi 15596084 260 FILYTSGSTGKPKGVLHT 277
Cdd:cd05927 118 TICYTSGTTGNPKGVMLT 135
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
82-616 |
3.22e-06 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 50.55 E-value: 3.22e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 82 LDRHLAERGDQVAIIWEGDDPADHQEITYRQLHEQVCKFANALRGQDVHrGDVVTIYMPMIPEAVVAMLACTRIGAIhsv 161
Cdd:PRK05691 15 LQRRAAQTPDRLALRFLADDPGEGVVLSYRDLDLRARTIAAALQARASF-GDRAVLLFPSGPDYVAAFFGCLYAGVI--- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 162 VFGGFSPEA--------LAGRIIDCKSKVVITaDEGVRGGKRTPLKANVDDAltnPETSSVqkiivckrtgaeikwnqhr 233
Cdd:PRK05691 91 AVPAYPPESarrhhqerLLSIIADAEPRLLLT-VADLRDSLLQMEELAAANA---PELLCV------------------- 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 234 dvwyDDLMKVAGSTCAPKEMGAEDPLFILYTSGSTGKPKGVlHTTGGYLVYASLTHERVF--DYRPGEVY--WCTA--DI 307
Cdd:PRK05691 148 ----DTLDPALAEAWQEPALQPDDIAFLQYTSGSTALPKGV-QVSHGNLVANEQLIRHGFgiDLNPDDVIvsWLPLyhDM 222
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 308 GWVTGhtyiVYGPLANGATTILFEgvPNY----PdvTRVAKIIDKHKVNILYTAPTAIRAMMAE-GKAAVAGADGSSLRL 382
Cdd:PRK05691 223 GLIGG----LLQPIFSGVPCVLMS--PAYflerP--LRWLEAISEYGGTISGGPDFAYRLCSERvSESALERLDLSRWRV 294
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 383 LGSVGEPI-----------------NPEAWQWYY-------------------------ETVGQSRCpivdtwwQTETGA 420
Cdd:PRK05691 295 AYSGSEPIrqdslerfaekfaacgfDPDSFFASYglaeatlfvsggrrgqgipaleldaEALARNRA-------EPGTGS 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 421 CLM---TPLPGaHAMKPgsaakpffgVVPALVDNLG-NLI-EGAAEGNLVILDSW---PGQARTlFGDHDRfvDTYFKTf 492
Cdd:PRK05691 368 VLMscgRSQPG-HAVLI---------VDPQSLEVLGdNRVgEIWASGPSIAHGYWrnpEASAKT-FVEHDG--RTWLRT- 433
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 493 kgmyftGD-GARRDedGYYWITGRVDDVLNVSGHRM------GTAEVESAMVAHPKVAEAAVvgmqhDIKGQ-GIYVYVT 564
Cdd:PRK05691 434 ------GDlGFLRD--GELFVTGRLKDMLIVRGHNLypqdieKTVEREVEVVRKGRVAAFAV-----NHQGEeGIGIAAE 500
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*.
gi 15596084 565 LNSGVE---PSEALRQELKQWVrREIGPIATPDVIQWAPG-LPKTRSGKIMRRILR 616
Cdd:PRK05691 501 ISRSVQkilPPQALIKSIRQAV-AEACQEAPSVVLLLNPGaLPKTSSGKLQRSACR 555
|
|
| PRK06814 |
PRK06814 |
acyl-[ACP]--phospholipid O-acyltransferase; |
132-622 |
5.19e-06 |
|
acyl-[ACP]--phospholipid O-acyltransferase;
Pssm-ID: 235865 [Multi-domain] Cd Length: 1140 Bit Score: 49.96 E-value: 5.19e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 132 GDVVTIYMPMIPEAVVAMLACTRIGAIHSVVfgGFSpeALAGRIID-CKS---KVVITADEGVRGGKRTPLKANVDdalt 207
Cdd:PRK06814 682 GENVGVMLPNANGAAVTFFALQSAGRVPAMI--NFS--AGIANILSaCKAaqvKTVLTSRAFIEKARLGPLIEALE---- 753
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 208 npetssvqkiivckrtgaeikwNQHRDVWYDDLMkvAGSTCAPKEMG---------------AEDPLFILYTSGSTGKPK 272
Cdd:PRK06814 754 ----------------------FGIRIIYLEDVR--AQIGLADKIKGllagrfplvyfcnrdPDDPAVILFTSGSEGTPK 809
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 273 GVLHTTGGYLVYASLTHERVfDYRPGEVYWCTADI----GwVTGHTYIvygPLANGATTILFegvPNYPDVTRVAKIIDK 348
Cdd:PRK06814 810 GVVLSHRNLLANRAQVAARI-DFSPEDKVFNALPVfhsfG-LTGGLVL---PLLSGVKVFLY---PSPLHYRIIPELIYD 881
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 349 HKVNILYTAPTAIrammaEGKAAVAGA-DGSSLRLLGSVGEPINPEAWQWYYETVGQSrcpIVDTWWQTETGACLM--TP 425
Cdd:PRK06814 882 TNATILFGTDTFL-----NGYARYAHPyDFRSLRYVFAGAEKVKEETRQTWMEKFGIR---ILEGYGVTETAPVIAlnTP 953
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 426 LpgahAMKPGSAAKPFFGVVPAL-----VDNLGNL-IEGA--------AEGNLVI---LDSWpgqartlfgdHDrfvdty 488
Cdd:PRK06814 954 M----HNKAGTVGRLLPGIEYRLepvpgIDEGGRLfVRGPnvmlgylrAENPGVLeppADGW----------YD------ 1013
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 489 fktfkgmyfTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKGQGIyVYVTlnsg 568
Cdd:PRK06814 1014 ---------TGDIVTIDEEGFITIKGRAKRFAKIAGEMISLAAVEELAAELWPDALHAAVSIPDARKGERI-ILLT---- 1079
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 15596084 569 vEPSEALRQELKQWVR-REIGPIATPDVIQWAPGLPKTRSGKI----MRRILRKIAAAE 622
Cdd:PRK06814 1080 -TASDATRAAFLAHAKaAGASELMVPAEIITIDEIPLLGTGKIdyvaVTKLAEEAAAKP 1137
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
108-283 |
8.28e-06 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 49.07 E-value: 8.28e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 108 ITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEALAgRIIDCKSKVVITAD 187
Cdd:PLN02861 78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGITYVPLYDTLGANAVE-FIINHAEVSIAFVQ 156
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 188 EGVRGGKRTPLK---ANVDDALTNPETSSVQKIIVCKRTGAEIKWNQHrdvwydDLMKVAGSTCAPKEMGaeDPLFILYT 264
Cdd:PLN02861 157 ESKISSILSCLPkcsSNLKTIVSFGDVSSEQKEEAEELGVSCFSWEEF------SLMGSLDCELPPKQKT--DICTIMYT 228
|
170
....*....|....*....
gi 15596084 265 SGSTGKPKGVLHTTGGYLV 283
Cdd:PLN02861 229 SGTTGEPKGVILTNRAIIA 247
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
105-544 |
9.24e-05 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 45.59 E-value: 9.24e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 105 HQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPEalagriidckskvvi 184
Cdd:cd17647 18 TRSFTYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPA--------------- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 185 tadegvrggkrtplKANVDDALTNPetssvQKIIVCKRTGAeikwnqhrdvwyddlmkVAGSTCAPKemgaedplfILYT 264
Cdd:cd17647 83 --------------RQNIYLGVAKP-----RGLIVIRAAGV-----------------VVGPDSNPT---------LSFT 117
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 265 SGSTGKPKGVL--HTTGGYlvYASLTHERvFDYRPGEVYwctadigwvTGHTYIVYGPLANGATTILFEG----VPNYPD 338
Cdd:cd17647 118 SGSEGIPKGVLgrHFSLAY--YFPWMAKR-FNLSENDKF---------TMLSGIAHDPIQRDMFTPLFLGaqllVPTQDD 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 339 VT---RVAKIIDKHKVNILYTAPtairAMmaeGKAAVAGADGSSLRLLGS--VGEPINPE---AWQWYYETvgqsrCPIV 410
Cdd:cd17647 186 IGtpgRLAEWMAKYGATVTHLTP----AM---GQLLTAQATTPFPKLHHAffVGDILTKRdclRLQTLAEN-----VRIV 253
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 411 DTWWQTETGACLmtplpgAHAMKPGSAAKPFF-----GVVPA---------LVDN---------LGNLIE------GAAE 461
Cdd:cd17647 254 NMYGTTETQRAV------SYFEVPSRSSDPTFlknlkDVMPAgrgmlnvqlLVVNrndrtqicgIGEVGEiyvragGLAE 327
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 462 GNL--------VILDSW---PGQartlFGDHDRFVDTYFKTF-----KGMYFTGDGARRDEDGYYWITGRVDDVLNVSGH 525
Cdd:cd17647 328 GYRglpelnkeKFVNNWfvePDH----WNYLDKDNNEPWRQFwlgprDRLYRTGDLGRYLPNGDCECCGRADDQVKIRGF 403
|
490
....*....|....*....
gi 15596084 526 RMGTAEVESAMVAHPKVAE 544
Cdd:cd17647 404 RIELGEIDTHISQHPLVRE 422
|
|
| A_NRPS_MycA_like |
cd05908 |
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin ... |
255-393 |
1.22e-04 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS) similar to mycosubtilin synthase subunit A (MycA); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPS similar to mycosubtilin synthase subunit A (MycA). Mycosubtilin, which is characterized by a beta-amino fatty acid moiety linked to the circular heptapeptide Asn-Tyr-Asn-Gln-Pro-Ser-Asn, belongs to the iturin family of lipopeptide antibiotics. The mycosubtilin synthase subunit A (MycA) combines functional domains derived from peptide synthetases, amino transferases, and fatty acid synthases. Nonribosomal peptide synthetases are large multifunction enzymes that synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341234 [Multi-domain] Cd Length: 499 Bit Score: 45.17 E-value: 1.22e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 255 AEDPLFILYTSGSTGKPKGVLHTTGGyLVYASLTHERVFDYRPGEVY--W--CTADIGWVTGHtyivYGPLANGAT---- 326
Cdd:cd05908 105 ADELAFIQFSSGSTGDPKGVMLTHEN-LVHNMFAILNSTEWKTKDRIlsWmpLTHDMGLIAFH----LAPLIAGMNqylm 179
|
90 100 110 120 130 140
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 15596084 327 -TILFEGVPnypdvTRVAKIIDKHKVNILYTAPTAIRAMMAEGKAAVAGA-DGSSLRLLGSVGEPINPE 393
Cdd:cd05908 180 pTRLFIRRP-----ILWLKKASEHKATIVSSPNFGYKYFLKTLKPEKANDwDLSSIRMILNGAEPIDYE 243
|
|
| alpha_am_amid |
TIGR03443 |
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are ... |
105-275 |
1.84e-04 |
|
L-aminoadipate-semialdehyde dehydrogenase; Members of this protein family are L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), product of the LYS2 gene. It is also called alpha-aminoadipate reductase. In fungi, lysine is synthesized via aminoadipate. Currently, all members of this family are fungal.
Pssm-ID: 274582 [Multi-domain] Cd Length: 1389 Bit Score: 45.06 E-value: 1.84e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 105 HQEITYRQLHEQVCKFANALRGQDVHRGDVVTIYMPMIPEAVVAMLACTRIGAIHSVVFGGFSPE------------AL- 171
Cdd:TIGR03443 268 TRSFTYKQINEASNILAHYLLKTGIKRGDVVMIYAYRGVDLVVAVMGVLKAGATFSVIDPAYPPArqtiylsvakprALi 347
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 172 ----AGRI-------IDCKSKVV-------ITADEGVRGGKrtpLKANVDDALtnpetSSVQKiIVCKRTGAeikwnqhr 233
Cdd:TIGR03443 348 viekAGTLdqlvrdyIDKELELRteipalaLQDDGSLVGGS---LEGGETDVL-----APYQA-LKDTPTGV-------- 410
|
170 180 190 200
....*....|....*....|....*....|....*....|..
gi 15596084 234 dvwyddlmkVAGSTCAPKemgaedplfILYTSGSTGKPKGVL 275
Cdd:TIGR03443 411 ---------VVGPDSNPT---------LSFTSGSEGIPKGVL 434
|
|
| PRK05851 |
PRK05851 |
long-chain-fatty acid--ACP ligase MbtM; |
486-612 |
2.94e-04 |
|
long-chain-fatty acid--ACP ligase MbtM;
Pssm-ID: 180289 [Multi-domain] Cd Length: 525 Bit Score: 43.99 E-value: 2.94e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 486 DTYFKTFKGMYFTgdgarrdeDGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKVAEAAVVGMQHDIKG--QGIYVYV 563
Cdd:PRK05851 395 DDWFPTGDLGYLV--------DGGLVVCGRAKELITVAGRNIFPTEIERVAAQVRGVREGAVVAVGTGEGSarPGLVIAA 466
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|
gi 15596084 564 TLNSGVEPseALRQELKQWVRREIGpIATPDVIQWAPG-LPKTRSGKIMR 612
Cdd:PRK05851 467 EFRGPDEA--GARSEVVQRVASECG-VVPSDVVFVAPGsLPRTSSGKLRR 513
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
507-612 |
2.72e-03 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 40.76 E-value: 2.72e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 507 DGYYWITGRVDDVLNVSGHRMGTAEVESAMVAHPKV--AEAAVVGMQHDiKGQGIYVYVTLN-SGVEPSEALRQELKQWV 583
Cdd:PRK09192 451 DGYLYITGRAKDLIIINGRNIWPQDIEWIAEQEPELrsGDAAAFSIAQE-NGEKIVLLVQCRiSDEERRGQLIHALAALV 529
|
90 100 110
....*....|....*....|....*....|.
gi 15596084 584 RREIGpiaTPDVIQWAP--GLPKTRSGKIMR 612
Cdd:PRK09192 530 RSEFG---VEAAVELVPphSLPRTSSGKLSR 557
|
|
| PRK08043 |
PRK08043 |
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase; |
494-622 |
3.20e-03 |
|
bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase;
Pssm-ID: 181207 [Multi-domain] Cd Length: 718 Bit Score: 40.85 E-value: 3.20e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15596084 494 GMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRMGTAEVES-AMVAHPKVAEAAVVgmQHD-IKGQGIYVYVTlnsgveP 571
Cdd:PRK08043 591 GWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMVSLEMVEQlALGVSPDKQHATAI--KSDaSKGEALVLFTT------D 662
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|..
gi 15596084 572 SEALRQELKQWVRREIGP-IATPDVIQWAPGLPKTRSGKIMRRILRKIAAAE 622
Cdd:PRK08043 663 SELTREKLQQYAREHGVPeLAVPRDIRYLKQLPLLGSGKPDFVTLKSMVDEP 714
|
|
| |
