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Conserved domains on  [gi|15597068|ref|NP_250562|]
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protease LasA [Pseudomonas aeruginosa PAO1]

Protein Classification

M23 family metallopeptidase( domain architecture ID 11432770)

M23 family metallopeptidase lyses bacterial cell wall peptidoglycans

EC:  3.4.24.-
Gene Ontology:  GO:0046872|GO:0008237
MEROPS:  M23
PubMed:  36386627

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NlpD COG0739
Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell ...
 190-368 6.25e-18

Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell wall/membrane/envelope biogenesis];


:

Pssm-ID: 440502 [Multi-domain]  Cd Length: 196  Bit Score: 81.56  E-value: 6.25e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 190 AKVSAVQGGNPLGAYAQTFQRLFGTPAAELLQPSNRVARQLQAKAALAPPSNLMQLPWR--QGYSWQPngaHSNTGSGYP 267
Cdd:COG0739  20 AAGAAAAVAAAAAAAAAAAALAAAALAAAVSAAASAAAAAAAAAAAIALGSGAWPVKGRitSGFGYRR---HPVTGRRRF 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 268 YSSFD--ASYDWPrwgsatysVVAAHAGTVRVLSRCQ-----VRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAG 340
Cdd:COG0739  97 HKGIDiaAPTGTP--------VYAAADGTVVFAGWNGgygnlVIIDHGNGYTTLYAHLSSILVKVGQRVKAGQVIG-YVG 167

                       170       180
                ....*....|....*....|....*...
gi 15597068 341 NintalceGGSSTGPHLHFSLLYNGAFV 368
Cdd:COG0739 168 N-------TGRSTGPHLHFEVRVNGKPV 188
 
Name Accession Description Interval E-value
NlpD COG0739
Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell ...
 190-368 6.25e-18

Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440502 [Multi-domain]  Cd Length: 196  Bit Score: 81.56  E-value: 6.25e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 190 AKVSAVQGGNPLGAYAQTFQRLFGTPAAELLQPSNRVARQLQAKAALAPPSNLMQLPWR--QGYSWQPngaHSNTGSGYP 267
Cdd:COG0739  20 AAGAAAAVAAAAAAAAAAAALAAAALAAAVSAAASAAAAAAAAAAAIALGSGAWPVKGRitSGFGYRR---HPVTGRRRF 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 268 YSSFD--ASYDWPrwgsatysVVAAHAGTVRVLSRCQ-----VRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAG 340
Cdd:COG0739  97 HKGIDiaAPTGTP--------VYAAADGTVVFAGWNGgygnlVIIDHGNGYTTLYAHLSSILVKVGQRVKAGQVIG-YVG 167

                       170       180
                ....*....|....*....|....*...
gi 15597068 341 NintalceGGSSTGPHLHFSLLYNGAFV 368
Cdd:COG0739 168 N-------TGRSTGPHLHFEVRVNGKPV 188
M23_peptidase cd12797
M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; ...
 287-361 2.46e-16

M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; This model describes the metallopeptidase M23 family, which includes beta-lytic metallopeptidase and lysostaphin. Members of this family are zinc endopeptidases that lyse bacterial cell wall peptidoglycans; they cleave either the N-acylmuramoyl-Ala bond between the cell wall peptidoglycan and the cross-linking peptide (e.g. beta-lytic endopeptidase) or a bond within the cross-linking peptide (e.g. stapholysin, and lysostaphin). Beta-lytic metallopeptidase, formerly known as beta-lytic protease, has a preference for cleavage of Gly-X bonds and favors hydrophobic or apolar residues on either side. It inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent. Lysostaphin, produced by Staphylococcus genus, cleaves pentaglycine cross-bridges of cell wall peptidoglycan, acting as autolysins to maintain cell wall metabolism or as toxins and weapons against competing strains. Staphylolysin (also known as LasA) is implicated in a range of processes related to Pseudomonas virulence, including stimulating shedding of the ectodomain of cell surface heparan sulphate proteoglycan syndecan-1, and elastin degradation in connective tissue. Its active site is less constricted and contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules, possibly contributing to its activity against a wider range of substrates than those used by related lytic enzymes, consistent with its multiple roles in Pseudomonas virulence. The family includes members that do not appear to have the conserved zinc-binding site and might be lipoproteins lacking proteolytic activity.


Pssm-ID: 410984 [Multi-domain]  Cd Length: 85  Bit Score: 73.39  E-value: 2.46e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 287 VVAAHAGTVRVLSR-----CQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAGNintalceGGSSTGPHLHFSL 361
Cdd:cd12797  14 VYAAADGTVVFAGWdggygNYVIIDHGNGYYTLYAHLSSILVKVGQRVKKGQVIG-TVGN-------TGRSTGPHLHFEI 85
Peptidase_M23 pfam01551
Peptidase family M23; Members of this family are zinc metallopeptidases with a range of ...
 287-368 4.04e-13

Peptidase family M23; Members of this family are zinc metallopeptidases with a range of specificities. The peptidase family M23 is included in this family, these are Gly-Gly endopeptidases. Peptidase family M23 are also endopeptidases. This family also includes some bacterial lipoproteins such as Swiss:P33648 for which no proteolytic activity has been demonstrated. This family also includes leukocyte cell-derived chemotaxin 2 (LECT2) proteins. LECT2 is a liver-specific protein which is thought to be linked to hepatocyte growth although the exact function of this protein is unknown.


Pssm-ID: 460250 [Multi-domain]  Cd Length: 96  Bit Score: 64.87  E-value: 4.04e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068   287 VVAAHAGTVrVLSR------CQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAGNintalceGGSSTGPHLHFS 360
Cdd:pfam01551  16 VYAAADGVV-VFAGwlggygNLVIIDHGNGYSTLYAHLSSILVKVGQRVKAGQVIG-TVGS-------TGRSTGPHLHFE 86


                  ....*...
gi 15597068   361 LLYNGAFV 368
Cdd:pfam01551  87 IRKNGKPV 94
PRK11649 PRK11649
putative peptidase; Provisional
 286-364 5.65e-04

putative peptidase; Provisional


Pssm-ID: 236946 [Multi-domain]  Cd Length: 439  Bit Score: 41.96  E-value: 5.65e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068  286 SVVAAHAGTVRVLSRCQ-----VRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGVyAGNIntalcegGSSTGPHLHFS 360
Cdd:PRK11649 325 PVLAVGDGEVVVAKRSGaagnyVAIRHGRQYTTRYMHLRKLLVKPGQKVKRGDRIAL-SGNT-------GRSTGPHLHYE 396


                 ....
gi 15597068  361 LLYN 364
Cdd:PRK11649 397 VWIN 400
 
Name Accession Description Interval E-value
NlpD COG0739
Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell ...
 190-368 6.25e-18

Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contains LysM domain [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 440502 [Multi-domain]  Cd Length: 196  Bit Score: 81.56  E-value: 6.25e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 190 AKVSAVQGGNPLGAYAQTFQRLFGTPAAELLQPSNRVARQLQAKAALAPPSNLMQLPWR--QGYSWQPngaHSNTGSGYP 267
Cdd:COG0739  20 AAGAAAAVAAAAAAAAAAAALAAAALAAAVSAAASAAAAAAAAAAAIALGSGAWPVKGRitSGFGYRR---HPVTGRRRF 96

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 268 YSSFD--ASYDWPrwgsatysVVAAHAGTVRVLSRCQ-----VRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAG 340
Cdd:COG0739  97 HKGIDiaAPTGTP--------VYAAADGTVVFAGWNGgygnlVIIDHGNGYTTLYAHLSSILVKVGQRVKAGQVIG-YVG 167

                       170       180
                ....*....|....*....|....*...
gi 15597068 341 NintalceGGSSTGPHLHFSLLYNGAFV 368
Cdd:COG0739 168 N-------TGRSTGPHLHFEVRVNGKPV 188
M23_peptidase cd12797
M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; ...
 287-361 2.46e-16

M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins; This model describes the metallopeptidase M23 family, which includes beta-lytic metallopeptidase and lysostaphin. Members of this family are zinc endopeptidases that lyse bacterial cell wall peptidoglycans; they cleave either the N-acylmuramoyl-Ala bond between the cell wall peptidoglycan and the cross-linking peptide (e.g. beta-lytic endopeptidase) or a bond within the cross-linking peptide (e.g. stapholysin, and lysostaphin). Beta-lytic metallopeptidase, formerly known as beta-lytic protease, has a preference for cleavage of Gly-X bonds and favors hydrophobic or apolar residues on either side. It inhibits growth of sensitive organisms and may potentially serve as an antimicrobial agent. Lysostaphin, produced by Staphylococcus genus, cleaves pentaglycine cross-bridges of cell wall peptidoglycan, acting as autolysins to maintain cell wall metabolism or as toxins and weapons against competing strains. Staphylolysin (also known as LasA) is implicated in a range of processes related to Pseudomonas virulence, including stimulating shedding of the ectodomain of cell surface heparan sulphate proteoglycan syndecan-1, and elastin degradation in connective tissue. Its active site is less constricted and contains a five-coordinate zinc ion with trigonal bipyramidal geometry and two metal-bound water molecules, possibly contributing to its activity against a wider range of substrates than those used by related lytic enzymes, consistent with its multiple roles in Pseudomonas virulence. The family includes members that do not appear to have the conserved zinc-binding site and might be lipoproteins lacking proteolytic activity.


Pssm-ID: 410984 [Multi-domain]  Cd Length: 85  Bit Score: 73.39  E-value: 2.46e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 287 VVAAHAGTVRVLSR-----CQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAGNintalceGGSSTGPHLHFSL 361
Cdd:cd12797  14 VYAAADGTVVFAGWdggygNYVIIDHGNGYYTLYAHLSSILVKVGQRVKKGQVIG-TVGN-------TGRSTGPHLHFEI 85
Peptidase_M23 pfam01551
Peptidase family M23; Members of this family are zinc metallopeptidases with a range of ...
 287-368 4.04e-13

Peptidase family M23; Members of this family are zinc metallopeptidases with a range of specificities. The peptidase family M23 is included in this family, these are Gly-Gly endopeptidases. Peptidase family M23 are also endopeptidases. This family also includes some bacterial lipoproteins such as Swiss:P33648 for which no proteolytic activity has been demonstrated. This family also includes leukocyte cell-derived chemotaxin 2 (LECT2) proteins. LECT2 is a liver-specific protein which is thought to be linked to hepatocyte growth although the exact function of this protein is unknown.


Pssm-ID: 460250 [Multi-domain]  Cd Length: 96  Bit Score: 64.87  E-value: 4.04e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068   287 VVAAHAGTVrVLSR------CQVRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAGNintalceGGSSTGPHLHFS 360
Cdd:pfam01551  16 VYAAADGVV-VFAGwlggygNLVIIDHGNGYSTLYAHLSSILVKVGQRVKAGQVIG-TVGS-------TGRSTGPHLHFE 86


                  ....*...
gi 15597068   361 LLYNGAFV 368
Cdd:pfam01551  87 IRKNGKPV 94
SpoIIQ2 COG5821
Stage II sporulation protein SpoIIQ, clostridial version, metallopeptidase M23 family [Cell ...
 287-365 2.27e-10

Stage II sporulation protein SpoIIQ, clostridial version, metallopeptidase M23 family [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 444523 [Multi-domain]  Cd Length: 200  Bit Score: 59.65  E-value: 2.27e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 287 VVAAHAGTVRVLSR-----CQVRVTHPSGWATNYYHMD-QIQVSNGQQVSADTKLGVyAGNinTALCEggSSTGPHLHFS 360
Cdd:COG5821 110 VKAAADGVVVEVGKdpkygITVVIDHGNGIKTVYANLDsKIKVKVGQKVKKGQVIGK-VGS--TALFE--SSEGPHLHFE 184


                ....*
gi 15597068 361 LLYNG 365
Cdd:COG5821 185 VLKNG 189
EnvC COG4942
Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, ...
 216-368 7.41e-10

Septal ring factor EnvC, activator of murein hydrolases AmiA and AmiB [Cell cycle control, cell division, chromosome partitioning];


Pssm-ID: 443969 [Multi-domain]  Cd Length: 377  Bit Score: 60.16  E-value: 7.41e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 216 AAELLQPSNRVARQLQAKAALAPPSNLM----QLPW------RQGYSWQPNGAHSNTGSGYPYSSfdasydwprwGSAty 285
Cdd:COG4942 222 AEELEALIARLEAEAAAAAERTPAAGFAalkgKLPWpvsgrvVRRFGERDGGGGRNKGIDIAAPP----------GAP-- 289

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068 286 sVVAAHAGTVRVLSRCQ-----VRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGvYAGNintalceGGSSTGPHLHFS 360
Cdd:COG4942 290 -VRAVADGTVVYAGWLRgygnlVIIDHGGGYLTLYAHLSSLLVKVGQRVKAGQPIG-TVGS-------SGGQGGPTLYFE 360


                ....*...
gi 15597068 361 LLYNGAFV 368
Cdd:COG4942 361 LRKNGKPV 368
PRK11649 PRK11649
putative peptidase; Provisional
 286-364 5.65e-04

putative peptidase; Provisional


Pssm-ID: 236946 [Multi-domain]  Cd Length: 439  Bit Score: 41.96  E-value: 5.65e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15597068  286 SVVAAHAGTVRVLSRCQ-----VRVTHPSGWATNYYHMDQIQVSNGQQVSADTKLGVyAGNIntalcegGSSTGPHLHFS 360
Cdd:PRK11649 325 PVLAVGDGEVVVAKRSGaagnyVAIRHGRQYTTRYMHLRKLLVKPGQKVKRGDRIAL-SGNT-------GRSTGPHLHYE 396


                 ....
gi 15597068  361 LLYN 364
Cdd:PRK11649 397 VWIN 400
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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