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Conserved domains on  [gi|15599650|ref|NP_253144|]
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hypothetical protein PA4454 [Pseudomonas aeruginosa PAO1]

Protein Classification

outer membrane lipid asymmetry maintenance protein MlaD( domain architecture ID 10025210)

outer membrane lipid asymmetry maintenance protein MlaD is part of the MlaFEDB complex, a non-canonical ABC transporter involved in maintaining outer membrane asymmetry

Gene Ontology:  GO:1990531|GO:0015914|GO:0005548
PubMed:  19383799|27529189

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 27-146 2.27e-56

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


:

Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 173.05  E-value: 2.27e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650    27 RVSGLSVGNAGDTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVnNLPVDSTASILTAGL 106
Cdd:TIGR04430  26 KVGNLSSFSSGDTYTVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAVVTLSIDKDY-KLPDDSSASILTSGL 104

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 15599650   107 LGEKYIGISVGGDEDVLKDGSTIHDTQSALVLEDLIGKFL 146
Cdd:TIGR04430 105 LGEQYIGLSPGGDEDYLKDGDEIEDTQSAVVLESLIGKFL 144
 
Name Accession Description Interval E-value
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 27-146 2.27e-56

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 173.05  E-value: 2.27e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650    27 RVSGLSVGNAGDTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVnNLPVDSTASILTAGL 106
Cdd:TIGR04430  26 KVGNLSSFSSGDTYTVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAVVTLSIDKDY-KLPDDSSASILTSGL 104

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 15599650   107 LGEKYIGISVGGDEDVLKDGSTIHDTQSALVLEDLIGKFL 146
Cdd:TIGR04430 105 LGEQYIGLSPGGDEDYLKDGDEIEDTQSAVVLESLIGKFL 144
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 32-155 1.41e-29

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 108.26  E-value: 1.41e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650  32 SVGNAGDTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVnNLPVDSTASILTAGLLGEKY 111
Cdd:COG1463  30 GLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGYRVRVTLRIDPDV-PIPADSVATIRSSGLLGEKY 108

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*..
gi 15599650 112 IGISVGGDED-VLKDGSTIhDTQSALVLEDLIGKF--LLNSVNKDEA 155
Cdd:COG1463 109 VELSPGGDSGpPLADGDVI-PLESPVDLEDLLSQLspLLEALDPGAL 154
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 38-117 1.30e-17

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 72.62  E-value: 1.30e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650    38 DTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVNN-LPVDSTASILTAGLLGEKYIGISV 116
Cdd:pfam02470   1 RGYTITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLDPDGKGVRVTLRIDPDYDVlLPKDSRAGIRSLGLLGGKYIALDP 80


                  .
gi 15599650   117 G 117
Cdd:pfam02470  81 G 81
 
Name Accession Description Interval E-value
OM_asym_MlaD TIGR04430
outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are ...
 27-146 2.27e-56

outer membrane lipid asymmetry maintenance protein MlaD; Members of this protein family are the MlaD (maintenance of Lipid Asymmetry D) protein of an ABC transport system that seems to remove phospholipid from the outer leaflet of the Gram-negative bacterial outer membrane (OM), leaving only lipopolysaccharide in the outer leaflet. The Mla locus has long been associated with toluene tolerance, consistent with the proposed role in retrograde transport of phospholipid and therefore with maintaining the integrity of the OM as a protective barrier.


Pssm-ID: 275223 [Multi-domain]  Cd Length: 146  Bit Score: 173.05  E-value: 2.27e-56
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650    27 RVSGLSVGNAGDTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVnNLPVDSTASILTAGL 106
Cdd:TIGR04430  26 KVGNLSSFSSGDTYTVTASFDNIGGLKVGAPVKIAGVKVGRVTDISLDPKTYQAVVTLSIDKDY-KLPDDSSASILTSGL 104

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 15599650   107 LGEKYIGISVGGDEDVLKDGSTIHDTQSALVLEDLIGKFL 146
Cdd:TIGR04430 105 LGEQYIGLSPGGDEDYLKDGDEIEDTQSAVVLESLIGKFL 144
MlaD COG1463
Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall ...
 32-155 1.41e-29

Periplasmic subunit MlaD of the ABC-type intermembrane phospholipid transporter Mla [Cell wall/membrane/envelope biogenesis];


Pssm-ID: 441072 [Multi-domain]  Cd Length: 260  Bit Score: 108.26  E-value: 1.41e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650  32 SVGNAGDTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVnNLPVDSTASILTAGLLGEKY 111
Cdd:COG1463  30 GLGLFGDTYTYTAVFDDVGGLKVGSPVRYAGVKVGRVTSIELDPDGYRVRVTLRIDPDV-PIPADSVATIRSSGLLGEKY 108

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*..
gi 15599650 112 IGISVGGDED-VLKDGSTIhDTQSALVLEDLIGKF--LLNSVNKDEA 155
Cdd:COG1463 109 VELSPGGDSGpPLADGDVI-PLESPVDLEDLLSQLspLLEALDPGAL 154
MlaD pfam02470
MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, ...
 38-117 1.30e-17

MlaD protein; This family of proteins contains MlaD, which is a component of the Mla pathway, an ABC transport system that functions to maintain the asymmetry of the outer membrane. The family also contains the mce (mammalian cell entry) proteins from Mycobacterium tuberculosis. The archetype (Rv0169), was isolated as being necessary for colonization of, and survival within, the macrophage. This family contains proteins of unknown function from other bacteria.


Pssm-ID: 426790 [Multi-domain]  Cd Length: 81  Bit Score: 72.62  E-value: 1.30e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650    38 DTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYTGRVTMEINQNVNN-LPVDSTASILTAGLLGEKYIGISV 116
Cdd:pfam02470   1 RGYTITAYFDDAGGLEVGAPVRYRGVPVGRVTSIDLDPDGKGVRVTLRIDPDYDVlLPKDSRAGIRSLGLLGGKYIALDP 80


                  .
gi 15599650   117 G 117
Cdd:pfam02470  81 G 81
Mtu_fam_mce TIGR00996
virulence factor Mce family protein; Members of this paralogous family are found as six tandem ...
 38-154 2.30e-16

virulence factor Mce family protein; Members of this paralogous family are found as six tandem homologous proteins in the same orientation per cassette, in four separate cassettes in Mycobacterium tuberculosis. The six members of each cassette represent six subfamilies. One subfamily includes the protein mce (mycobacterial cell entry), a virulence protein required for invasion of non-phagocytic cells. [Cellular processes, Pathogenesis]


Pssm-ID: 273384 [Multi-domain]  Cd Length: 291  Bit Score: 73.86  E-value: 2.30e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15599650    38 DTYKVYAYFDNIAGVTVRGKVTLAGVTIGKVTAVDLDRDSYtgRVTMEINQNVnNLPVDSTASILTAGLLGEKYIGISVG 117
Cdd:TIGR00996  31 GTTTVVAYFTDAGGLYPGDKVRVAGVPVGKVTAIEPRGARA--RVTFSLDRSV-TLPANATAAIRTTTLLGSRYIELTPP 107

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 15599650   118 GDE--DVLKDGSTIH--DTQSALVLEDLIGKF--LLNSVNKDE 154
Cdd:TIGR00996 108 GGPpgGRLSDGGTIPlsRTTVPLELDDLLGSLtrLLNGLDPEK 150
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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