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Conserved domains on  [gi|15600461|ref|NP_253955|]
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magnesium/cobalt transporter [Pseudomonas aeruginosa PAO1]

Protein Classification

magnesium transporter CorA family protein( domain architecture ID 10191309)

magnesium transporter CorA family protein belonging to the CorA metal ion transporter (MIT) superfamily, similar to CorA which mediates both influx and efflux of magnesium, and may also transport nickel and cobalt

CATH:  3.30.460.20|1.20.58.340
Gene Ontology:  GO:0046873|GO:0030001
PubMed:  17619822|23046658|16179994
SCOP:  4000404|3000358

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EcCorA-like_u1 cd12837
uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A ...
 29-326 1.22e-166

uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A uncharacterized subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA_ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA_ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213371 [Multi-domain]  Cd Length: 298  Bit Score: 465.47  E-value: 1.22e-166
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  29 LWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGGITEHRPSTEEVTFVLSEKWLITVRY 108
Cdd:cd12837   1 VWIDLLNPTPEEEAAVEALLGIAIPTREEMSEIEASSRLYREGGALYMTAPLLSRADTDDPELSPVTFVLTPERLVTVRY 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 109 ADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKPKTDLQQIIKHLGR 188
Cdd:cd12837  81 ADPRAFDTFAARAARQPSGCASGEDVLLGLLEAIVDRLADVLERVGADLDRLSRRIFRSSAKRRRRASRDLRSVLRRIGR 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 189 SSSLLSKLNDSLLSINRMNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNSIIKV 268
Cdd:cd12837 161 AGDLLSKIRESLLSLGRLLSFLSANAADWLSKELRARLKTLQRDVASLSEHATFLSNKIQFLLDATLGLINIEQNNIIKI 240

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 15600461 269 FSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12837 241 FSVASVVFLPPTLIASIYGMNFKHMPELDWPFGYPLALGLMVLSAVLPLLWFKRRGWL 298
 
Name Accession Description Interval E-value
EcCorA-like_u1 cd12837
uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A ...
 29-326 1.22e-166

uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A uncharacterized subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA_ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA_ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213371 [Multi-domain]  Cd Length: 298  Bit Score: 465.47  E-value: 1.22e-166
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  29 LWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGGITEHRPSTEEVTFVLSEKWLITVRY 108
Cdd:cd12837   1 VWIDLLNPTPEEEAAVEALLGIAIPTREEMSEIEASSRLYREGGALYMTAPLLSRADTDDPELSPVTFVLTPERLVTVRY 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 109 ADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKPKTDLQQIIKHLGR 188
Cdd:cd12837  81 ADPRAFDTFAARAARQPSGCASGEDVLLGLLEAIVDRLADVLERVGADLDRLSRRIFRSSAKRRRRASRDLRSVLRRIGR 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 189 SSSLLSKLNDSLLSINRMNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNSIIKV 268
Cdd:cd12837 161 AGDLLSKIRESLLSLGRLLSFLSANAADWLSKELRARLKTLQRDVASLSEHATFLSNKIQFLLDATLGLINIEQNNIIKI 240

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 15600461 269 FSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12837 241 FSVASVVFLPPTLIASIYGMNFKHMPELDWPFGYPLALGLMVLSAVLPLLWFKRRGWL 298
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
25-326 1.21e-84

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 257.45  E-value: 1.21e-84
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  25 PPGTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTT-SVVGGITEHRPSTEEVTFVLSEKWL 103
Cdd:COG0598  12 PEGFVWIDLDGPTDEELEWLAEEFGLHPLALEDALDIEQRPKLEEYDDYLFIVLrTLNYNEEEEELETGPLSIFLGPNFL 91

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 104 ITVRYADLRSFREFENRCIRQLD-ANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTaqtrkpktdlQQI 182
Cdd:COG0598  92 ITVRERPLDAFDDVRERLERGPGlLPRGPDYLLYALLDAIVDRYFPVLEELEDELDELEDELFESPD----------NEL 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 183 IKHLGRSSSLLSKLNDSLLSINRMNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQ 262
Cdd:COG0598 162 LRRLYRLRRELVRLRRALAPLREVLSRLLRGESPLISEEDREYLRDVYDHLERLLEMVDSLRERLSGLLDAYLSLISNRQ 241

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15600461 263 NSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:COG0598 242 NEIMKVLTIIAAIFLPPTFIAGIYGMNFEGMPELDWPYGYPIVLGLMLLIAVLLYLYFKRKGWL 305
PRK11085 PRK11085
magnesium/cobalt transporter CorA;
1-326 2.38e-53

magnesium/cobalt transporter CorA;


Pssm-ID: 182951  Cd Length: 316  Bit Score: 177.61  E-value: 2.38e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    1 MITYYILSGDRLERRQHgDAQRGLPPgTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSV 80
Cdd:PRK11085   1 MLSAFQLENNRLTRLEV-EESQPLVD-AVWIDLVEPDDDERLRVQSELGQSLATRPELEDIEASARFFEDEDGLHIHSFF 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   81 VGGITEHRPSTEEVTFVLSEKWLITVRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSM 160
Cdd:PRK11085  79 FFEDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQTLVDGNAYELLLDLFETKIEQLADEIENIYSDLEKL 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  161 SREIFaestaqTRKPKTDLQQIIKHLGRSSSLLSKLNDSLLSINR-MNSYFRQGcngwlSEPAKVL--AKTVERDVRSLD 237
Cdd:PRK11085 159 SRVIM------EGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRaLNFLVRKA-----RLPGGQLeqAREILRDIESLL 227

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  238 EYLAKMSTEINFLLDATLGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPY 317
Cdd:PRK11085 228 PHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIILMILAGLAPY 307


                 ....*....
gi 15600461  318 LWFKFKNWL 326
Cdd:PRK11085 308 LYFKRKNWL 316
corA TIGR00383
magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp ...
 29-326 1.10e-47

magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp Genomics 1998;3(3):151-69 discusses this family and suggests that some members may have functions other than Mg2+ transport. [Transport and binding proteins, Cations and iron carrying compounds]


Pssm-ID: 273048 [Multi-domain]  Cd Length: 318  Bit Score: 162.94  E-value: 1.10e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    29 LWIDLHHPTPEEE----------RLLESHLQVNIPTReemaeiedsSRFYEDGNVLYMTTSVVGGITEHRPSTEEVTFVL 98
Cdd:TIGR00383  28 LWIDLIEPTDEERlaklgqffaiHPLALEDILNSPQR---------PKVEEDEDHLFIISFFLNEDEDDTFETEQVSFIL 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    99 SEKWLITVRYADLRSFREFENRC-----IRQLDANrsstQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFaestaqtR 173
Cdd:TIGR00383  99 GKNLLFTIHERELPAFDSIRERIrtsqkVFEKGAD----YLLYDIFDAIIDSYFPLLENIEDELEELEDEII-------S 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   174 KPKTDLQQIIKHLgrsSSLLSKLNDSLLSINR-MNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLD 252
Cdd:TIGR00383 168 GPTSTLMDEILSL---RTELLALRRSLWPLRDvLNFLLRKTHLPIQTEEVREYLRDIYDHILSLLEMIETYRELLSSLMD 244

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15600461   253 ATLGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:TIGR00383 245 LYLSLVNNKMNEIMKILTVVSTIFIPLTFIAGIYGMNFKFMPELNWKYGYPAVLIVMAVIALGPLIYFRRKGWL 318
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 27-321 8.27e-47

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 159.79  E-value: 8.27e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    27 GTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGGITEHRPSTE-EVTFVLSEKWLIT 105
Cdd:pfam01544   3 GFVWLDLDSPTEAELEWLARTLGLDPLALEDLLDPEERPRVEDYDDYFLLVLPGVNLDDSEEADELvTVSILLGDNFLIT 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   106 VRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKpktDLQQIIKH 185
Cdd:pfam01544  83 VRHEPLPALDEVRKRLTLLGGGPLDPGDLLYRLLDEIVDRFLELLEKLEDELDELEDELEDETNNELLR---ELAALRRS 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   186 LGrsssllsKLNDSLLSINRM-NSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNS 264
Cdd:pfam01544 160 LV-------YLRRSLLPQRDVlNRLLRREDDPLLDDEQKEYLRDLLDRLERLLEDLDALRERLRSLQDEYSSLLSERTNR 232

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 15600461   265 IIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMA--LGMMVVSAILPYLWFK 321
Cdd:pfam01544 233 VMKVLTVVTAIFLPLTLITGIYGMNFGGLPELEWSYGYPFVliLGLMILLAILLLFYFR 291
 
Name Accession Description Interval E-value
EcCorA-like_u1 cd12837
uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A ...
 29-326 1.22e-166

uncharacterized subgroup of the Escherichia coli Mg2+ transporter CorA_like subfamily; A uncharacterized subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA_ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA_ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213371 [Multi-domain]  Cd Length: 298  Bit Score: 465.47  E-value: 1.22e-166
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  29 LWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGGITEHRPSTEEVTFVLSEKWLITVRY 108
Cdd:cd12837   1 VWIDLLNPTPEEEAAVEALLGIAIPTREEMSEIEASSRLYREGGALYMTAPLLSRADTDDPELSPVTFVLTPERLVTVRY 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 109 ADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKPKTDLQQIIKHLGR 188
Cdd:cd12837  81 ADPRAFDTFAARAARQPSGCASGEDVLLGLLEAIVDRLADVLERVGADLDRLSRRIFRSSAKRRRRASRDLRSVLRRIGR 160

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 189 SSSLLSKLNDSLLSINRMNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNSIIKV 268
Cdd:cd12837 161 AGDLLSKIRESLLSLGRLLSFLSANAADWLSKELRARLKTLQRDVASLSEHATFLSNKIQFLLDATLGLINIEQNNIIKI 240

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*...
gi 15600461 269 FSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12837 241 FSVASVVFLPPTLIASIYGMNFKHMPELDWPFGYPLALGLMVLSAVLPLLWFKRRGWL 298
CorA COG0598
Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];
25-326 1.21e-84

Mg2+ and Co2+ transporter CorA [Inorganic ion transport and metabolism];


Pssm-ID: 440363 [Multi-domain]  Cd Length: 305  Bit Score: 257.45  E-value: 1.21e-84
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  25 PPGTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTT-SVVGGITEHRPSTEEVTFVLSEKWL 103
Cdd:COG0598  12 PEGFVWIDLDGPTDEELEWLAEEFGLHPLALEDALDIEQRPKLEEYDDYLFIVLrTLNYNEEEEELETGPLSIFLGPNFL 91

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 104 ITVRYADLRSFREFENRCIRQLD-ANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTaqtrkpktdlQQI 182
Cdd:COG0598  92 ITVRERPLDAFDDVRERLERGPGlLPRGPDYLLYALLDAIVDRYFPVLEELEDELDELEDELFESPD----------NEL 161

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 183 IKHLGRSSSLLSKLNDSLLSINRMNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQ 262
Cdd:COG0598 162 LRRLYRLRRELVRLRRALAPLREVLSRLLRGESPLISEEDREYLRDVYDHLERLLEMVDSLRERLSGLLDAYLSLISNRQ 241

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15600461 263 NSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:COG0598 242 NEIMKVLTIIAAIFLPPTFIAGIYGMNFEGMPELDWPYGYPIVLGLMLLIAVLLYLYFKRKGWL 305
EcCorA-like cd12825
Escherichia coli Mg2+ transporter CorA_like subfamily; A bacterial subfamily of the ...
 30-326 2.11e-83

Escherichia coli Mg2+ transporter CorA_like subfamily; A bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB_like(EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Mg2+ transporters Escherichia coli, Salmonella typhimurium, and Helicobacter pylori CorAs (which can also transport Co2+, and Ni2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213359 [Multi-domain]  Cd Length: 287  Bit Score: 253.78  E-value: 2.11e-83
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGGITEHRPSTEEVTFVLSEKWLITVRYA 109
Cdd:cd12825   1 WIDLINPTEEELATLSSEYGFILPPREHLEDIEISSRYWEDDSQLFITLSFPNQTKEDTIEEEPVTFIIKGNILFTIRYR 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 110 DLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAEstaqtrkpKTDLQQIIKHLGRS 189
Cdd:cd12825  81 DLPAFDIFPKRAFSQRKFFNSGEELFLFLFEVIVDYLADILENISKKIKQLSREILEE--------KQFSEEDLDALTKL 152

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 190 SSLLSKLNDSLLSINRMNSYFRQGcnGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNSIIKVF 269
Cdd:cd12825 153 NENNITLRESLIEKQRIITLLLKS--PRENSDTKEKLKIELRDLSSLLDHTTFNFDKLDDLKNNILGKIELEQNKIIKIF 230

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 15600461 270 SIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12825 231 TVVTVCFLPPTLIASIYGMNFKNMPELKWSYGYPFALILMILSTILPLLYFKRKGWL 287
EcCorA-like_1 cd12835
Escherichia coli Mg2+ transporter CorA_like subgroup; A bacterial subgroup of the Escherichia ...
 30-326 1.37e-79

Escherichia coli Mg2+ transporter CorA_like subgroup; A bacterial subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Mg2+ transporters Escherichia coli CorA and Salmonella typhimurium CorA (which can also transport Co2+, and Ni2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213369  Cd Length: 287  Bit Score: 244.09  E-value: 1.37e-79
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTtSVVGGITEHRPSTEEVTFVLSEKWLITVRYA 109
Cdd:cd12835   1 WIDLIAPDDEEREWLEQLLGQELPDLEDLEEIEASARFYVDEDGLHIH-SLFLQDSEGRARNVTVAFTLRDDRLITLREE 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 110 DLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAqtrkpktDLQQIIKHLGRS 189
Cdd:cd12835  80 ELPVFRLLRLRARRGPGEVSDPKDLLLDLFEQKVEYLADIIEDIYRDLERVSREVLEENDE-------DLEEALGRLARQ 152

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 190 SSLLSKLNDSLLSINRMNSYFRQGCNGWLSEPAKvlAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNSIIKVF 269
Cdd:cd12835 153 EDLNGKIRLCLMDTQRALSFLLRTRRLSDEQLED--AREILRDIESLLPHTQFLFDKINFLMDAAMGFINIEQNKIIKIF 230

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 15600461 270 SIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12835 231 SVAAVVFLPPTLIASIYGMNFDFMPELDWKFGYPMAIGLMILSAIAPYLYFKRKGWL 287
HpCorA-like cd12836
Mg2+ transporter Helicobacter pylori CorA-like subgroup; A bacterial subgroup of the ...
 28-326 2.86e-74

Mg2+ transporter Helicobacter pylori CorA-like subgroup; A bacterial subgroup of the Escherichia coli CorA-Salmonella typhimurium ZntB_like (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Mg2+ transporter Helicobacter pylori CorAs (which can also transport Co2+, and Ni2+); CorA plays an important role in the viability of this pathogen. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB (members of the EcCorA_ZntB-like family) form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213370 [Multi-domain]  Cd Length: 288  Bit Score: 230.22  E-value: 2.86e-74
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  28 TLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGgITEHRPSTEEVTFVLSEKWLITVR 107
Cdd:cd12836   1 VLWIDLINPTEEEIKTVEQYFNIEFPTKQESEEIESSSRYWEDEDSITINSNFLV-PDDDGYHNEPVSFILKDNILFTIR 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 108 YADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTrkpktDLQQIIKHLg 187
Cdd:cd12836  80 YGELRTFSETSKKLRANPKKFQTGYDIFSSILEIRIDYDADLLENISKEISKLRKKLFADEDLDE-----ELLEKISRL- 153

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 188 rsSSLLSKLNDSLLSINRMNSYFRQgcNGWLSEPAKVLAKTVERDVRSLDEYlakmsTEINF-----LLDATLGLINIEQ 262
Cdd:cd12836 154 --QELNMTIRENLIDKQRVLSSLLK--SEKFPEDLKENLRIMIKDINSLIEH-----TTFNFerldyLQNTFLGLINIEQ 224

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15600461 263 NSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12836 225 NKIIKIFTVATVIFMPPTLIASIYGMNFKFMPELDWKYGYPFALLLMVISSILPLLYFKKKKWL 288
PRK11085 PRK11085
magnesium/cobalt transporter CorA;
1-326 2.38e-53

magnesium/cobalt transporter CorA;


Pssm-ID: 182951  Cd Length: 316  Bit Score: 177.61  E-value: 2.38e-53
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    1 MITYYILSGDRLERRQHgDAQRGLPPgTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSV 80
Cdd:PRK11085   1 MLSAFQLENNRLTRLEV-EESQPLVD-AVWIDLVEPDDDERLRVQSELGQSLATRPELEDIEASARFFEDEDGLHIHSFF 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   81 VGGITEHRPSTEEVTFVLSEKWLITVRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSM 160
Cdd:PRK11085  79 FFEDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQTLVDGNAYELLLDLFETKIEQLADEIENIYSDLEKL 158

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  161 SREIFaestaqTRKPKTDLQQIIKHLGRSSSLLSKLNDSLLSINR-MNSYFRQGcngwlSEPAKVL--AKTVERDVRSLD 237
Cdd:PRK11085 159 SRVIM------EGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRaLNFLVRKA-----RLPGGQLeqAREILRDIESLL 227

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  238 EYLAKMSTEINFLLDATLGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPY 317
Cdd:PRK11085 228 PHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIILMILAGLAPY 307


                 ....*....
gi 15600461  318 LWFKFKNWL 326
Cdd:PRK11085 308 LYFKRKNWL 316
corA TIGR00383
magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp ...
 29-326 1.10e-47

magnesium Mg(2+) and cobalt Co(2+) transport protein (corA); The article in Microb Comp Genomics 1998;3(3):151-69 discusses this family and suggests that some members may have functions other than Mg2+ transport. [Transport and binding proteins, Cations and iron carrying compounds]


Pssm-ID: 273048 [Multi-domain]  Cd Length: 318  Bit Score: 162.94  E-value: 1.10e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    29 LWIDLHHPTPEEE----------RLLESHLQVNIPTReemaeiedsSRFYEDGNVLYMTTSVVGGITEHRPSTEEVTFVL 98
Cdd:TIGR00383  28 LWIDLIEPTDEERlaklgqffaiHPLALEDILNSPQR---------PKVEEDEDHLFIISFFLNEDEDDTFETEQVSFIL 98

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    99 SEKWLITVRYADLRSFREFENRC-----IRQLDANrsstQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFaestaqtR 173
Cdd:TIGR00383  99 GKNLLFTIHERELPAFDSIRERIrtsqkVFEKGAD----YLLYDIFDAIIDSYFPLLENIEDELEELEDEII-------S 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   174 KPKTDLQQIIKHLgrsSSLLSKLNDSLLSINR-MNSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLD 252
Cdd:TIGR00383 168 GPTSTLMDEILSL---RTELLALRRSLWPLRDvLNFLLRKTHLPIQTEEVREYLRDIYDHILSLLEMIETYRELLSSLMD 244

                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15600461   253 ATLGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:TIGR00383 245 LYLSLVNNKMNEIMKILTVVSTIFIPLTFIAGIYGMNFKFMPELNWKYGYPAVLIVMAVIALGPLIYFRRKGWL 318
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 27-321 8.27e-47

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 159.79  E-value: 8.27e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461    27 GTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGGITEHRPSTE-EVTFVLSEKWLIT 105
Cdd:pfam01544   3 GFVWLDLDSPTEAELEWLARTLGLDPLALEDLLDPEERPRVEDYDDYFLLVLPGVNLDDSEEADELvTVSILLGDNFLIT 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   106 VRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKpktDLQQIIKH 185
Cdd:pfam01544  83 VRHEPLPALDEVRKRLTLLGGGPLDPGDLLYRLLDEIVDRFLELLEKLEDELDELEDELEDETNNELLR---ELAALRRS 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461   186 LGrsssllsKLNDSLLSINRM-NSYFRQGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNS 264
Cdd:pfam01544 160 LV-------YLRRSLLPQRDVlNRLLRREDDPLLDDEQKEYLRDLLDRLERLLEDLDALRERLRSLQDEYSSLLSERTNR 232

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 15600461   265 IIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMA--LGMMVVSAILPYLWFK 321
Cdd:pfam01544 233 VMKVLTVVTAIFLPLTLITGIYGMNFGGLPELEWSYGYPFVliLGLMILLAILLLFYFR 291
TmCorA-like cd12822
Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential ...
 30-326 5.59e-41

Thermotoga maritima CorA-like family; This family belongs to the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the Thermotoga maritima CorA_like family are found in all three kingdoms of life. It is a functionally diverse family, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213356 [Multi-domain]  Cd Length: 289  Bit Score: 144.25  E-value: 5.59e-41
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNiptreEMAeIEDSSRFYE-------DGNVLYMTTSVVGGITEHRPSTEEVTFVLSEKW 102
Cdd:cd12822   1 WIDLEGPTEEELQLLAEEFGFH-----PLA-IEDALNERQrpkieeyDDYLFLVLRALRYDEEELELETSELSIFLGKNF 74

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 103 LITVRYADLRSFREFENRCIRQLDANRSSTQLFALM-MDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKP----KT 177
Cdd:cd12822  75 LVTVHEGPLRLIDEVIERLLSDPKLLPKGPDFLLYAiLDAIVDSYFPILDELEDRLDELEDEILEEPRREVLRElfalRR 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 178 DLQQIIKHLGRSSSLLSKLNDSLLSINR--MNSYFRQgcngwlsepakvLAKTVERDVRSLDEYLAKMSTeinfLLDATL 255
Cdd:cd12822 155 ELLRLRRILSPQREVLNRLLRRENELISeeLRPYFRD------------VYDHLERLAELLDSYRDLLSS----LLDAYL 218

                       250       260       270       280       290       300       310
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15600461 256 GLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12822 219 SLQSNRTNEVMKVLTIITTIFLPLTFITGIYGMNFEYMPELHWPYGYFIVLGIMLLIAGGLLWWFRRKKWL 289
EcCorA_ZntB-like cd12821
Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily ...
 30-325 1.41e-37

Escherichia coli CorA-Salmonella typhimurium ZntB_like family; A family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this family are found in all three kingdoms of life. It is a functionally diverse family, including the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), and the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). It also includes two Saccharomyces cerevisiae members: the inner membrane Mg2+ transporters Mfm1p/Lpe10p, and Mrs2p, and a family of Arabidopsis thaliana members (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213355 [Multi-domain]  Cd Length: 285  Bit Score: 135.52  E-value: 1.41e-37
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYE--DGNVLYMTTSVVGGITEHRPsteeVTFVLSEKWLITVR 107
Cdd:cd12821   1 WYDIDEPDDEIRRLLEELESSHLPNDLRDILLGVSFPRFEevSGSGIYIQLRKPQKDADTAS----FHFLITRDRLITVR 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 108 YADLRSFREFENRCIRQLDAN-RSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKPKTDLQQIIKHL 186
Cdd:cd12821  77 LDELPALDELRQDLRSGQMKSaKSAAELLFVLLGAIIKALLTGIDQFEEKLEELEWDLLEGNNAIKLDRILELRRELLRL 156

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 187 GRSSSLLSKLNDSLLSInrmnsyFRQGCNGWLSEPAKVLAKTVERDVRSLDEYlakmSTEINFLLDATLGLINIEQNSII 266
Cdd:cd12821 157 TNLIEPQQEVLMALQEA------FAELLFSEDEEELRRTLDRIERLLQLIEEY----EQELDTLQDIEEVVSSHRGNEIM 226

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 15600461 267 KVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNW 325
Cdd:cd12821 227 KTLTVVTTVFLPLTLIGGLWGMNFKNMPELEWKYGYLFVLGLIILSTAGLYLYLRRKGW 285
MtCorA-like cd12830
Mycobacterium tuberculosis CorA-like subfamily; This bacterial subfamily belongs to the ...
 29-326 8.43e-36

Mycobacterium tuberculosis CorA-like subfamily; This bacterial subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Mg2+ transporter Mycobacterium tuberculosis CorA (which also transports Co2+). Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213364 [Multi-domain]  Cd Length: 292  Bit Score: 130.72  E-value: 8.43e-36
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  29 LWIDLHHPTPEEERLLESHLQVniptrEEMAeIEDSSR--------FYEDGNVLYMTTSVVGGITEhRPSTEEVTFVLSE 100
Cdd:cd12830   3 VWVGLHEPDAEELAAVAEEFGL-----HELA-VEDAVHahqrpkleRYGDSLFLVLRTARYVDHTE-EVETGETHVFVGP 75

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 101 KWLITVRYADLRSFREFENRCIRQLDANR-SSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAestaqtRKPKTDL 179
Cdd:cd12830  76 DFVVTVRHGAAGPLARVRARLEADPELLAlGPDAVLYAILDFIVDGYLPVAEELEDDVDELEEEVFS------PDRRDDI 149

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 180 QQI------IKHLGRS----SSLLSKL-NDSLLSINR-MNSYFRQgcngwlsepakVL--AKTVERDVRSLDEYLakmst 245
Cdd:cd12830 150 ERIyrlkreVLEFRRAvdplVEVLRRLaRGHEPLIPKeLRPYFRD-----------VAdhLLRVADRVDSLRELL----- 213

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 246 eiNFLLDATLGLINIEQNSIIKVFS----IAAVlflpPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFK 321
Cdd:cd12830 214 --SSALQANLALVSVQQNEDMRKISawaaILAV----PTAIAGIYGMNFDHMPELHWTYGYPLVLGLMVAVCLGLYRRFR 287


                ....*
gi 15600461 322 FKNWL 326
Cdd:cd12830 288 RNGWL 292
MIT_CorA-like cd11744
metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of ...
 30-325 2.02e-34

metal ion transporter CorA-like divalent cation transporter superfamily; This superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. They are found in most bacteria and archaea, and in some eukaryotes. It is a functionally diverse group which includes the Mg2+ transporters of Escherichia coli and Salmonella typhimurium CorAs (which can also transport Co2+, and Ni2+ ), the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and the Zn2+ transporter Salmonella typhimurium ZntB, which mediates the efflux of Zn2+ (and Cd2+). It includes five Saccharomyces cerevisiae members: i) two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, ii) two mitochondrial inner membrane Mg2+ transporters: Mfm1p/Lpe10p, and Mrs2p, and iii) and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. It also includes a family of Arabidopsis thaliana members (AtMGTs), some of which are localized to distinct tissues, and not all of which can transport Mg2+. Thermotoga maritima CorA and Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, Mrs2p, and Alr1p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213354 [Multi-domain]  Cd Length: 286  Bit Score: 127.32  E-value: 2.02e-34
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMttsVVGGITE----HRPSTEEVTFVLSEKWLIT 105
Cdd:cd11744   1 WIHCERLHDDDIRRVLEDNLHVPRALEDILLAESRPSFHPLDDNFMI---ILRGFNMdenaSPEDMLSVRILYFQGALIS 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 106 VRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREI----FAESTAQT--------- 172
Cdd:cd11744  78 TREIPSRAFMEIRQRLREHSKGPKSAASLLNQLIEGLNGKIDLYLETIEETLNELDVNDeeghYDESTYNHialqkalis 157

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 173 -RKPKTDLQQIIKHLGRSSSLLSKlndsllsinRMNSYFRQgcngwlsepakvlaktVERDVRSLDEYLAKMSTEINFLL 251
Cdd:cd11744 158 iRRFIRPQQYALRDLIRSESELVT---------SRPHQYRF----------------AHNNITRINETIEFYLGEVALLQ 212

                       250       260       270       280       290       300       310
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15600461 252 DATLGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNW 325
Cdd:cd11744 213 DEIKHNRDEKTNKNSKLFTLVATIFLPTTFLAGLYGMNFEGMPGVESSMAFTWFCIALIVAFGLEWLLFKRKGF 286
TmCorA-like_u3 cd12832
Uncharacterized subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs ...
 30-326 6.09e-27

Uncharacterized subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the TmCorA-like family are found in all three kingdoms of life. It is a functionally diverse family which includes the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and three Saccharomyces cerevisiae proteins: two located in the plasma membrane: the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213366 [Multi-domain]  Cd Length: 287  Bit Score: 107.30  E-value: 6.09e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVVGG-ITEHRPSTEEVT----FVLSEKWLI 104
Cdd:cd12832   1 WIDVVAPTEEDLEDLARRYNLHETSLKDCLDPDHLPKYEEVDGVTFIILRVYTDkKRENADTIQELTtkiaIFVVDKFVI 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 105 TVRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTaqtrkpKTDLQQIIK 184
Cdd:cd12832  81 TIHRTDLEFIANLKEKWLSGMHKCESPTELLLRIVKDALKTYEAPLEKLSEELDKYEEEVFLKKR------KGSLLKRLY 154

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 185 HLGRSSSLLSK-LNDSLLSINRMNSYFrqgcngwlsepakvLAKTVERDVRSLdeyLAKMSTEINFLLDATLGLINI--- 260
Cdd:cd12832 155 HLKRKASVIKRvLNLTQDVIDKIDTPL--------------KSDPYTQDLRDL---LIKLLSLYDELHENATNLLSLyis 217

                       250       260       270       280       290       300       310
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 261 ----EQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12832 218 lssqRTNEVMRVLTIFSVFFLPLTFIAGVYGMNFKYMPELHWKYGYPATLLVMIVIVVGIYFWFKRKGWL 287
TmCorA-like_1 cd12828
Thermotoga maritima CorA_like subfamily; This subfamily belongs to the Thermotoga maritima ...
 27-326 1.77e-25

Thermotoga maritima CorA_like subfamily; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of this subfamily are found in all three kingdoms of life. It is functionally diverse subfamily, in addition to the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, it includes Methanosarcina mazei CorA which may be involved in transport of copper and/or other divalent metal ions. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213362 [Multi-domain]  Cd Length: 294  Bit Score: 103.28  E-value: 1.77e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  27 GTLWIDLH--HPTPEEERLLEsHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTTSVV-GGITEHRPSTEEVTFVLSEKWL 103
Cdd:cd12828   1 GVTWINVDglHDIELIEKLGE-RFGLHPLVLEDILNTGQRPKVEEYDDYLFIVLKMLyYDEDTGELESEQVSLILGKNYV 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 104 ITVRYADLRSFREFENRC------IRQLDANRsstqLFALMMDSLVDRIADVLEAVQGQLSSMSREIFaestaqtRKPKT 177
Cdd:cd12828  80 ISFQERPGDVFDPVRERLrngkgrIRKKGADY----LAYALLDAIVDNYFPVLEKLGDRIEDLEEELL-------ENPTP 148

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 178 DLQQIIKHLGRSSSLLSK----LNDSLLSINRMNSYFrqgcngwLSEPAKVLAktveRDV-----RSLD--EYLAKMSTE 246
Cdd:cd12828 149 ETLERIHKLKRELLFLRRsiwpLREVINRLLRGESPL-------ISEETRPYL----RDVydhtiQVIDtiETYRDMLSG 217

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 247 infLLDATLGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12828 218 ---LLDLYLSSVSNRMNEVMKVLTIISTIFIPLTFIAGIYGMNFEYMPELEWKYGYPAVLGVMLLIALGMLLYFKRKKWL 294
TmCorA-like_u2 cd12831
Uncharacterized bacterial subfamily of the Thermotoga maritima CorA-like family; This ...
 30-326 1.37e-23

Uncharacterized bacterial subfamily of the Thermotoga maritima CorA-like family; This subfamily belongs to the Thermotoga maritima CorA (TmCorA)-like family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. Members of the TmCorA-like family are found in all three kingdoms of life. It is a functionally diverse family which includes the CorA Co2+ transporter from the hyperthermophilic Thermotoga maritima, and three Saccharomyces cerevisiae proteins: two located in the plasma membrane: the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by a related protein, Saccharomyces cerevisiae Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213365 [Multi-domain]  Cd Length: 287  Bit Score: 98.00  E-value: 1.37e-23
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNiPTreemaEIEDS----SR----FYEDGNVLymttsVVGGITEHRPSTEEVTFVLSEK 101
Cdd:cd12831   4 WVDFNNPTEEEIKLLTSFFHFH-PL-----AIEDClhllQRpkldYYDDYQFL-----VLHAIDQEDLEAEEVNLFVGEN 72

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 102 WLITVRYADLRSFREFENRCIRQLDANRSSTQLFALMMDSLVD-------RIADVL-----EAVQGQLSSMSREIFaest 169
Cdd:cd12831  73 YIVTFHKEPIREINEVWEKLKEKKDKKLGTLYIAYMIIDKIVDdyfpivyEIEDRLneiedNNVDSSNKKLMDEVF---- 148

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 170 aqtrkpktDLQQIIKHLGRS-------------SSLLSKLNDSLLSINRMNSYFRQgcngwlsepakvLAKTVE------ 230
Cdd:cd12831 149 --------DIRSDLLKLRRTilpmrdllyrilnSERLEGDKEQRAYFQDIYDHLLK------------LSEMLEsnremt 208

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 231 RDVRslDEYLAKMSTEINflldatlglinieqnSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMV 310
Cdd:cd12831 209 ADIR--DNYESYNSNRMN---------------SIMMTLTIISTIFMPLTFIAGIYGMNFEYMPELKWHYGYFVVLGIML 271

                       330
                ....*....|....*.
gi 15600461 311 VSAILPYLWFKFKNWL 326
Cdd:cd12831 272 LIAIGMILWFKKKGWF 287
EcCorA_ZntB-like_u1 cd12826
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 128-326 1.31e-16

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. The EcCorA-ZntB_like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB_like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213360  Cd Length: 281  Bit Score: 78.38  E-value: 1.31e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 128 NRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFaestaqTRKPKTDLQQIIKHLGRSSSL------LSKLNDSLL 201
Cdd:cd12826  93 GPSLERFLYDFLEYLIKDDLEYLEKYEEKLEELEEEIL------DGKLKNFNERILKIRKELLRLnsyyeqLMDIGEELE 166

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 202 SinRMNSYFRQG-CNGWlsepaKVLAKTVER---DVRSLDEYlakmSTEINFLLDAtlgLINIEQNSIIKVFSIAAVLFL 277
Cdd:cd12826 167 E--NENDLFDEEeLRLF-----RLFSDRVERlhdNVQMLREY----SLQIRELYQS---QIDIRQNRIMKILTVVTTIFL 232

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*....
gi 15600461 278 PPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12826 233 PLTLITGWYGMNFTNMPELHWKYGYPVVIILSILIVVIEIIYFKKKKWL 281
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 30-326 1.59e-14

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 72.54  E-value: 1.59e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMT----TSVVGGITEHRPSTEEVTFVLSEKWLIT 105
Cdd:cd12827   1 WINVVNPTEEEIEQLAEKYGIPEDFLTYALDEEERSRIEYDDDTDYLLiildVPVEEEEDDDEYETIPLGIILTKDRLIT 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 106 VRYADLRSFREFENRCIRQLDaNRSSTQLFALMMDSLVDRIADVLEavqgQLSSMSREIfaESTAQTRKPKTDLQQIIKh 185
Cdd:cd12827  81 ICNHETDLLDDFLEGRVKNFD-TSSKTRFLLQILYRIATSYLKYLR----QINKERDEL--EQELRKSTKNKELFQLLD- 152

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 186 LGRSS-SLLSKLNDSLLSINRMNSYFRQgcngWLSEPAKVLAKTVERDVRSLDEyLAKMSTEI-NFLLDATLGLINIEQN 263
Cdd:cd12827 153 LEKSLvYFSTSLKSNEIVLEKLKRSRSL----KLDEEDKELLEDVIIENKQAIE-MAEIYSDIlSGLMDAFASVISNNLN 227

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 15600461 264 SIIKVFSIAAVLFLPPTLVGTVYGMNFKdMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12827 228 NVMKFLTSITIVLTIPTIITGFYGMNVP-LPFADSPYAFWIILLISLLLSVIVALILRRKKYF 289
ZntB-like cd12824
Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; A bacterial subfamily belonging ...
 27-321 3.37e-11

Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; A bacterial subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213358 [Multi-domain]  Cd Length: 290  Bit Score: 62.92  E-value: 3.37e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  27 GTLWIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYE-DGNVLYMTTSVVGGITEHRPSTEEVTFVLSEKWLIT 105
Cdd:cd12824   1 GNTWYHCDRADPAIRSWLEDNTHLPEATVDHLLAPVRFPHFEPvDDGFLLSLRGIRMDEAASPSDMADLRILIGQGAAVT 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 106 VRYADLRSFREFEnRCIRQLDANRSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKPKTDLQQIIKH 185
Cdd:cd12824  81 VRSGPSRAVDELR-RALRDHSSLKSLADLLAQMISGMTKRLELVIFDLTETLDEVEDNLLDGGTAPPPYALIALQRRLFR 159

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 186 LGRsssllsKLNDSLLSINRMnsyfrqgcngwLSEPAKVLAKT-----------VERDVRSLDEYLakmsTEINFLLDAT 254
Cdd:cd12824 160 IRR------QIRPQQQALRPL-----------ITDPAELLVTSdretlrrasnnVTRYLETLDFCR----TRVALLQDEI 218

                       250       260       270       280       290       300
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 15600461 255 LGLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFK 321
Cdd:cd12824 219 KAQRDEKTTRSSLLLTVVATVFLPLTFITGLLGMNVGGIPDVESPMGFWWVTGLSILVALLAWLLLR 285
ZntB-like_1 cd12833
Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to ...
 103-326 1.78e-10

Salmonella typhimurium Zn2+ transporter ZntB-like subgroup; A bacterial subgroup belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subgroup includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213367 [Multi-domain]  Cd Length: 290  Bit Score: 60.59  E-value: 1.78e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 103 LITVRYADLRSFREFenrcIRQLDAN---RSSTQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAESTAQTRKPKTDL 179
Cdd:cd12833  78 IISTRRRRLLAVDDI----REALEAGkgpKSPGDFLAALAERLTDRMEDVIDELEDRLDELEERVLEEEDEELRGELAEL 153

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 180 Q-QIIK---HLGRSSSLLSKLndSLLSINRMNSYFRQgcngWLSEPAKVLAKTVErDVRSLDEYLAKMSTEINflldatl 255
Cdd:cd12833 154 RrQAIAlrrYLAPQRDALERL--AREDLPWLSDDDRL----RLREAADRLTRYIE-DLDAIRERAAVLQEELT------- 219

                       170       180       190       200       210       220       230
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 15600461 256 GLINIEQNSIIKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFKFKNWL 326
Cdd:cd12833 220 NRRAEQMNRRMYVLSIVAAIFLPLGFLTGLLGINVGGIPGAENPWAFWIFCGLLVVLAVGLLLLFRRKKWL 290
Alr1p-like cd12829
Saccharomyces cerevisiae Alr1p-like subfamily; This eukaryotic subfamily belongs to the ...
 30-318 1.43e-08

Saccharomyces cerevisiae Alr1p-like subfamily; This eukaryotic subfamily belongs to the Thermotoga maritima CorA (TmCorA)-family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This subfamily includes three Saccharomyces cerevisiae members: two plasma membrane proteins, the Mg2+ transporter Alr1p/Swc3p and the putative Mg2+ transporter, Alr2p, and the vacuole membrane protein Mnr2p, a putative Mg2+ transporter. Thermotoga maritima CorA forms funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport by Alr1p. Natural variants in this signature sequence may be associated with the transport of different divalent cations. The functional diversity of the MIT superfamily may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213363 [Multi-domain]  Cd Length: 305  Bit Score: 54.96  E-value: 1.43e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  30 WIDLHHPTPEEERLLESHLQVNIPTREEMAEIEDSSRFYE-DGNVLYMTTSVVGGITEHRPSTEEVTF--VLSEKWLITV 106
Cdd:cd12829   1 WLDVQSPTDDDMRAIGKLFPIHPLTLEDILSQDTREKVELfPNYYFISFRAIESDSELDENVLEPVNVyiVVFREGILTF 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 107 RYADLRSFREFENRcIRQLDAN---RSSTQLFALMmDSLVDRIADVLEAVQ---GQLSSMSREIFAEST-------AQTR 173
Cdd:cd12829  81 HFKPLDHIERVRKR-IRLLSDShllSSDWVLYALL-DSIVDSFFPLVDSIEkevDSIDELVLLISSSDQsdllrriGDLR 158

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 174 K----------PKTD-LQQIIKHLGRSSSLLSK----------LNDSLLSINRMNSYfrqgcngwlsepakvlaktveRD 232
Cdd:cd12829 159 RrvtsllrllfAKSDvLKQLLKRLMKKTFVSPDsevaiylgdvQDHILTLLQKLNHY---------------------ED 217

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 233 V--RSLDEYLAKMSTEINFLldatlgliNIEQNSIIKVFSIAAVLFLPPTLVGTVYGMN----FKDMPELDWSIGYPMAL 306
Cdd:cd12829 218 LlsRSHSNYLAQLSLEMSQE--------SNGTDKAMLRLTVIATIFLPLQLITGLFGMNvkvpGQDIESLYWFFGIVIVA 289

                       330
                ....*....|..
gi 15600461 307 GMMVVSAILPYL 318
Cdd:cd12829 290 IMVLVAIVVVFL 301
ZntB_u1 cd12834
Uncharacterized bacterial subgroup of the Salmonella typhimurium Zn2+ transporter ZntB-like ...
 41-321 2.00e-06

Uncharacterized bacterial subgroup of the Salmonella typhimurium Zn2+ transporter ZntB-like subfamily; The MIT superfamily of essential membrane proteins is involved in transporting divalent cations (uptake or efflux) across membranes. The ZntB-like subfamily includes the Zn2+ transporter Salmonella typhimurium ZntB which mediates the efflux of Zn2+ (and Cd2+). Structures of the intracellular domain of Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN which occur in proteins belonging to this subfamily, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213368 [Multi-domain]  Cd Length: 290  Bit Score: 48.35  E-value: 2.00e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461  41 ERLLESHLQVNIPTREEMAEIEDSSRFYEDGNVLYMTtsVVGGITEHRPSTEEV---TFVLSEKWLITVRYADLRSfref 117
Cdd:cd12834  15 RQWIAAHPALPEAARALLLSGDTSTRLEVDDDALYGV--LNDLERDFAEASTDVgtlRFAVTPRLLITARRQPLRS---- 88

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 118 enrcirqLDANRSStQLFALMMDSLVDRIADVLEAVQGQLSSMSREIFAEstaqtrkpktdLQQIIKHL-----GRSSSL 192
Cdd:cd12834  89 -------VDRLRQA-VERGLRFRSPADLLEHLLREQADTLSRIVRELTDE-----------LDRIEDRLlsgrlRRERRE 149

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15600461 193 LSKLNDSLLSINRM-----NSYFR--QGCNGWLSEPAKVLAKTVERDVRSLDEYLAKMSTEINFLLDATLGLINIEQNSI 265
Cdd:cd12834 150 LGALRRRLVRLHRLlapepAALFRllRRPPDWLPDADLETLRRLTERLDALDRDLVALQERARLLQEEIAARLAEQTNRS 229

                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 15600461 266 IKVFSIAAVLFLPPTLVGTVYGMNFKDMPELDWSIGYPMALGMMVVSAILPYLWFK 321
Cdd:cd12834 230 LYILSVVTALLLPPTLVTGLFGMNTGGLPLAEGPHGFWIAVALALLAAGLAYLLLR 285
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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