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Conserved domains on  [gi|15829484|ref|NP_308257|]
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type VI secretion protein [Escherichia coli O157:H7 str. Sakai]

Protein Classification

type VI secretion system baseplate subunit TssE( domain architecture ID 10022389)

type VI secretion system (T6SS) baseplate subunit TssE is a component of the T6SS baseplate that serves not only as the tail assembly platform, but also docks the tail to the membrane complex

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
VI_zyme TIGR03357
type VI secretion system lysozyme-like protein; The description for Pfam family pfam04965 ...
 9-136 4.46e-43

type VI secretion system lysozyme-like protein; The description for Pfam family pfam04965 cites acidic lysozyme activity for some phage-encoded members. This family represents a different subgroup of the proteins from pfam04965, where all members are associated with bacterial type VI secretion system genomic contexts.


:

Pssm-ID: 274540  Cd Length: 133  Bit Score: 138.13  E-value: 4.46e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484     9 TGSLFERMEASSA--RNRQGGSIHSLRQSIRQNLRNILNTRSGSCRGAPELGI----DEPEGAENFRESMSRAIEQCIER 82
Cdd:TIGR03357   1 TPSLFERLPDASGesPSTRRSSAEQLRESIRRHLERLLNTRRGSCASLPDYGLpdlnDLSLSSADDRRRIRRAIEQAIER 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 15829484    83 YEPRISHAEVQAvVSSASSPLDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRV 136
Cdd:TIGR03357  81 YEPRLSRVRVTA-LPDEEDPLALRFRIEAELDVDGGPEPVSFDALLDPSGRYRV 133
 
Name Accession Description Interval E-value
VI_zyme TIGR03357
type VI secretion system lysozyme-like protein; The description for Pfam family pfam04965 ...
 9-136 4.46e-43

type VI secretion system lysozyme-like protein; The description for Pfam family pfam04965 cites acidic lysozyme activity for some phage-encoded members. This family represents a different subgroup of the proteins from pfam04965, where all members are associated with bacterial type VI secretion system genomic contexts.


Pssm-ID: 274540  Cd Length: 133  Bit Score: 138.13  E-value: 4.46e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484     9 TGSLFERMEASSA--RNRQGGSIHSLRQSIRQNLRNILNTRSGSCRGAPELGI----DEPEGAENFRESMSRAIEQCIER 82
Cdd:TIGR03357   1 TPSLFERLPDASGesPSTRRSSAEQLRESIRRHLERLLNTRRGSCASLPDYGLpdlnDLSLSSADDRRRIRRAIEQAIER 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 15829484    83 YEPRISHAEVQAvVSSASSPLDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRV 136
Cdd:TIGR03357  81 YEPRLSRVRVTA-LPDEEDPLALRFRIEAELDVDGGPEPVSFDALLDPSGRYRV 133
COG3518 COG3518
Predicted component of the type VI protein secretion system [Intracellular trafficking, ...
 5-136 7.32e-24

Predicted component of the type VI protein secretion system [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442740  Cd Length: 162  Bit Score: 90.04  E-value: 7.32e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484   5 RERTTGSLFER------MEASSARNRQGGSIHSLRQSIRQNLRNILNTRSGSC----------RGAPELGI----DEPEG 64
Cdd:COG3518   7 RRRLQPSLLDRltddepDRSRESPLSRVVSLEQLRESVRRDLEWLLNTRRGEAsllaaypevrRSVLNYGLpdlsGASLG 86

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15829484  65 AENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSPLDMTFHITAWVTFNETHEVLEFD--MAPNgSQHYRV 136
Cdd:COG3518  87 SPDDREAIERAIRQAILRFEPRLRRVSVTVLPDDDDSPNTLRFRIEAVLWADPAPEPVRFDtvLDLE-TGRVSV 159
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 31-113 1.68e-16

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 69.53  E-value: 1.68e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484    31 SLRQSIRQNLRNILNTRSGSCRGAPELGIDEPEGA-----ENFRESMSRAIEQCIERYEPRISHAEVQAVVSSAsSPLDM 105
Cdd:pfam04965   2 SLRESVRRDLEILLNTRPGERVSRPNYGCPDLALLfapndDATRALIERAIREAILRFEPRIRVVSVEVDPDPD-SPGTL 80


                  ....*...
gi 15829484   106 TFHITAWV 113
Cdd:pfam04965  81 RFTIEYTL 88
25 PHA00415
baseplate wedge subunit
 35-93 2.56e-04

baseplate wedge subunit


Pssm-ID: 222788  Cd Length: 131  Bit Score: 38.44  E-value: 2.56e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15829484   35 SIRQNLRNILNTRSGSCRGAPELGIDEPEGA-ENFR----ESMSRAIEQCIERYEPRISHAEVQ 93
Cdd:PHA00415  32 AIKNSLLGIVTTRKGERPFDPNFGCDISDQLfENMDpltaDTIERNIESAIRNYEPRVYNLNVE 95
 
Name Accession Description Interval E-value
VI_zyme TIGR03357
type VI secretion system lysozyme-like protein; The description for Pfam family pfam04965 ...
 9-136 4.46e-43

type VI secretion system lysozyme-like protein; The description for Pfam family pfam04965 cites acidic lysozyme activity for some phage-encoded members. This family represents a different subgroup of the proteins from pfam04965, where all members are associated with bacterial type VI secretion system genomic contexts.


Pssm-ID: 274540  Cd Length: 133  Bit Score: 138.13  E-value: 4.46e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484     9 TGSLFERMEASSA--RNRQGGSIHSLRQSIRQNLRNILNTRSGSCRGAPELGI----DEPEGAENFRESMSRAIEQCIER 82
Cdd:TIGR03357   1 TPSLFERLPDASGesPSTRRSSAEQLRESIRRHLERLLNTRRGSCASLPDYGLpdlnDLSLSSADDRRRIRRAIEQAIER 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 15829484    83 YEPRISHAEVQAvVSSASSPLDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRV 136
Cdd:TIGR03357  81 YEPRLSRVRVTA-LPDEEDPLALRFRIEAELDVDGGPEPVSFDALLDPSGRYRV 133
COG3518 COG3518
Predicted component of the type VI protein secretion system [Intracellular trafficking, ...
 5-136 7.32e-24

Predicted component of the type VI protein secretion system [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 442740  Cd Length: 162  Bit Score: 90.04  E-value: 7.32e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484   5 RERTTGSLFER------MEASSARNRQGGSIHSLRQSIRQNLRNILNTRSGSC----------RGAPELGI----DEPEG 64
Cdd:COG3518   7 RRRLQPSLLDRltddepDRSRESPLSRVVSLEQLRESVRRDLEWLLNTRRGEAsllaaypevrRSVLNYGLpdlsGASLG 86

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15829484  65 AENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSPLDMTFHITAWVTFNETHEVLEFD--MAPNgSQHYRV 136
Cdd:COG3518  87 SPDDREAIERAIRQAILRFEPRLRRVSVTVLPDDDDSPNTLRFRIEAVLWADPAPEPVRFDtvLDLE-TGRVSV 159
GPW_gp25 pfam04965
Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like ...
 31-113 1.68e-16

Baseplate wedge protein gp25; This family represents T4 phage gp25 protein and gp25-like proteins, found in several systems, including contractile tail bacteriophages, the type VI secretion system (T6SS) and R-type pyocins, which constitute a multiprotein tubular apparatus to attach to and penetrate host cell membranes. Gp25 is a component of the conserved wedge in the inner part of the baseplate and serves as a nucleus for sheath polymerization, playing a critical role in sheath assembly and contraction. The EPR motif (Glu-Pro-Arg, residues 85-87 of gp25) is conserved across all members of the family including orthologs from the RpoS-mediated general stress response system (called IraD). This motif interacts with the 'core bundle' composed of orthologs of T4 gp6 and gp7 proteins in contractile injection systems.


Pssm-ID: 428225  Cd Length: 93  Bit Score: 69.53  E-value: 1.68e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484    31 SLRQSIRQNLRNILNTRSGSCRGAPELGIDEPEGA-----ENFRESMSRAIEQCIERYEPRISHAEVQAVVSSAsSPLDM 105
Cdd:pfam04965   2 SLRESVRRDLEILLNTRPGERVSRPNYGCPDLALLfapndDATRALIERAIREAILRFEPRIRVVSVEVDPDPD-SPGTL 80


                  ....*...
gi 15829484   106 TFHITAWV 113
Cdd:pfam04965  81 RFTIEYTL 88
COG3628 COG3628
Phage baseplate assembly protein W [Mobilome: prophages, transposons];
23-110 1.64e-08

Phage baseplate assembly protein W [Mobilome: prophages, transposons];


Pssm-ID: 442846  Cd Length: 119  Bit Score: 49.48  E-value: 1.64e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484  23 NRQGGSIHSLR--QSIRQNLRNILNTRSGSCRGAPELG------IDEPEGAEnFRESMSRAIEQCIERYEPRIshaEVQA 94
Cdd:COG3628   7 DRATGRVAMVSgeEHIRQSIRIILTTPPGERVMRPDFGsglhdlLFEPNNPA-TALLIRAAVAEALLRWEPRI---EVES 82

                        90
                ....*....|....*.
gi 15829484  95 VVSSASSPLDMTFHIT 110
Cdd:COG3628  83 VDVTPDPEEDGKLLIT 98
25 PHA00415
baseplate wedge subunit
 35-93 2.56e-04

baseplate wedge subunit


Pssm-ID: 222788  Cd Length: 131  Bit Score: 38.44  E-value: 2.56e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 15829484   35 SIRQNLRNILNTRSGSCRGAPELGIDEPEGA-ENFR----ESMSRAIEQCIERYEPRISHAEVQ 93
Cdd:PHA00415  32 AIKNSLLGIVTTRKGERPFDPNFGCDISDQLfENMDpltaDTIERNIESAIRNYEPRVYNLNVE 95
W PHA02516
baseplate wedge subunit; Provisional
 23-112 6.91e-04

baseplate wedge subunit; Provisional


Pssm-ID: 134016  Cd Length: 103  Bit Score: 36.60  E-value: 6.91e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15829484   23 NRQGGSIHSLRQSIRQNLRNILNTRSGSCRGAPELG------IDEPEgAENFRESMSRAIEQCIERYEPRISHAEVQAvv 96
Cdd:PHA02516   2 NRETGRALSDLEHIRQSIGDILLTPLGSRVMRREYGsllpdlIDQPQ-NPALRLQIYAACAMALMRWEPRITLTRVQI-- 78

                         90
                 ....*....|....*.
gi 15829484   97 sSASSPLDMTFHITAW 112
Cdd:PHA02516  79 -ERAADGRMTVDITGW 93
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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