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Conserved domains on  [gi|15830104|ref|NP_308877|]
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T3SS secreted effector NleD [Escherichia coli O157:H7 str. Sakai]

Protein Classification

type III secretion system effector protein( domain architecture ID 141806)

type III secretion system (T3SS) effector protein is a M91 family metallopeptidase, similar to Escherichia coli T3SS effector NleD, a zinc metalloprotease that is translocated into host enterocytes, where it directly cleaves and inactivates the mitogen-activated protein kinase signaling proteins JNK and p38

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Peptidase_M91 super family cl20781
Effector protein; This family of proteins contains an HEXXH motif, typical of zinc ...
 38-206 5.43e-24

Effector protein; This family of proteins contains an HEXXH motif, typical of zinc metallopeptidases. The family includes the E. coli effector protein NleD, which cleaves and inactivates c-Jun N-terminal kinase (JNK).


The actual alignment was detected with superfamily member pfam14891:

Pssm-ID: 473508  Cd Length: 173  Bit Score: 94.02  E-value: 5.43e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15830104    38 NNPQLRFQGTDhNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIH-LNSSRLGVMAHrdiDAENHRGTGSDFH 116
Cdd:pfam14891   1 NNRFIKVEGSD-EFKDRVRADLDMLRSSPTGQQMLNELSSSGSETRRKLTIKeIVDSDNGYAAY---GGDPGEGTDVIIN 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15830104   117 CNLNAVEYPCGEGISvvdfhATIVFHELLHVFHNLNGERLKVESSRAESQKYSPLLLEEARTVGLG------------AF 184
Cdd:pfam14891  77 WNPNFSSINGNEGRP-----TTVLYHELAHAYDFLNGTLDDGRYDGPDPPDTSGVPNEERQAVGLPhdgdpktpdvrdPD 151

                         170       180
                  ....*....|....*....|..
gi 15830104   185 SEEVLSENKFHEEIGMPRRTSY 206
Cdd:pfam14891 152 HPYEPTENGLRAELGLPLRPHY 173
 
Name Accession Description Interval E-value
Peptidase_M91 pfam14891
Effector protein; This family of proteins contains an HEXXH motif, typical of zinc ...
 38-206 5.43e-24

Effector protein; This family of proteins contains an HEXXH motif, typical of zinc metallopeptidases. The family includes the E. coli effector protein NleD, which cleaves and inactivates c-Jun N-terminal kinase (JNK).


Pssm-ID: 405561  Cd Length: 173  Bit Score: 94.02  E-value: 5.43e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15830104    38 NNPQLRFQGTDhNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIH-LNSSRLGVMAHrdiDAENHRGTGSDFH 116
Cdd:pfam14891   1 NNRFIKVEGSD-EFKDRVRADLDMLRSSPTGQQMLNELSSSGSETRRKLTIKeIVDSDNGYAAY---GGDPGEGTDVIIN 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15830104   117 CNLNAVEYPCGEGISvvdfhATIVFHELLHVFHNLNGERLKVESSRAESQKYSPLLLEEARTVGLG------------AF 184
Cdd:pfam14891  77 WNPNFSSINGNEGRP-----TTVLYHELAHAYDFLNGTLDDGRYDGPDPPDTSGVPNEERQAVGLPhdgdpktpdvrdPD 151

                         170       180
                  ....*....|....*....|..
gi 15830104   185 SEEVLSENKFHEEIGMPRRTSY 206
Cdd:pfam14891 152 HPYEPTENGLRAELGLPLRPHY 173
 
Name Accession Description Interval E-value
Peptidase_M91 pfam14891
Effector protein; This family of proteins contains an HEXXH motif, typical of zinc ...
 38-206 5.43e-24

Effector protein; This family of proteins contains an HEXXH motif, typical of zinc metallopeptidases. The family includes the E. coli effector protein NleD, which cleaves and inactivates c-Jun N-terminal kinase (JNK).


Pssm-ID: 405561  Cd Length: 173  Bit Score: 94.02  E-value: 5.43e-24
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15830104    38 NNPQLRFQGTDhNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIH-LNSSRLGVMAHrdiDAENHRGTGSDFH 116
Cdd:pfam14891   1 NNRFIKVEGSD-EFKDRVRADLDMLRSSPTGQQMLNELSSSGSETRRKLTIKeIVDSDNGYAAY---GGDPGEGTDVIIN 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15830104   117 CNLNAVEYPCGEGISvvdfhATIVFHELLHVFHNLNGERLKVESSRAESQKYSPLLLEEARTVGLG------------AF 184
Cdd:pfam14891  77 WNPNFSSINGNEGRP-----TTVLYHELAHAYDFLNGTLDDGRYDGPDPPDTSGVPNEERQAVGLPhdgdpktpdvrdPD 151

                         170       180
                  ....*....|....*....|..
gi 15830104   185 SEEVLSENKFHEEIGMPRRTSY 206
Cdd:pfam14891 152 HPYEPTENGLRAELGLPLRPHY 173
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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