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Conserved domains on  [gi|15833109|ref|NP_311882|]
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T3SS secreted effector EspL [Escherichia coli O157:H7 str. Sakai]

Protein Classification

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Toxin_15 super family cl06788
ShET2 enterotoxin, N-terminal region; The members of this family are are sequences that are ...
21-266 6.28e-40

ShET2 enterotoxin, N-terminal region; The members of this family are are sequences that are similar to the N-terminal half of the ShET2 enterotoxin produced by Shigella flexneri and Escherichia coli. This protein was found to confer toxigenicity in the Ussing chamber, and the N-terminal region was found to be important for the protein's enterotoxic effect. It is thought to be a hydrophobic protein that forms inclusion bodies within the bacterial cell, and may be secreted by the Mxi system. Most members of this family are annotated as putative enterotoxins, but one member is a regulator of acetyl CoA synthetase, and another two members (Swiss:P76205 and Swiss:P23325) are annotated as ankyrin-like regulatory proteins and contain Ank repeats (pfam00023).


The actual alignment was detected with superfamily member pfam07906:

Pssm-ID: 369588  Cd Length: 278  Bit Score: 146.19  E-value: 6.28e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109    21 PYLSKKRDASINLNGKVSDCNGEIIWCRHIASYW-SEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGT----NNIKGDV 95
Cdd:pfam07906  22 PYFSEKKSFRINLNGQAYDRSGRLIVCRHLASQYiMDFLDEAKGKVDYKHFSSPDKIANHVSLEKKEQTyalfSSRPEGI 101
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109    96 YFVENESWGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKEN-KFVINFYDPNQTATHKRVFFctNNICDII 174
Cdd:pfam07906 102 YFIENGDFGLVLKAIFDKMESNGENVRALILLSSNHAMAVRLRIKETEEGcRYVVSVYDPNVTNTTVRCEV--DSLEKIK 179
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109   175 NLTAYDFLSEQ-------CLKCYGLKEDTLSLFVD--KTKSNDNNNVFIKKLPDNI--LQGVVINFAMGAGLREIIKKVy 243
Cdd:pfam07906 180 DYSLKDFINVDysllkysNDEIYGLISQGQAIIPDrhKPQLLSLKGSVDEISPLIKlpLSPAVLYLLMTIGFTEQIQQL- 258
                         250       260
                  ....*....|....*....|...
gi 15833109   244 ndtrftdltKSQMKILCESKNVN 266
Cdd:pfam07906 259 ---------MEQLQNISESHLTE 272
 
Name Accession Description Interval E-value
Toxin_15 pfam07906
ShET2 enterotoxin, N-terminal region; The members of this family are are sequences that are ...
21-266 6.28e-40

ShET2 enterotoxin, N-terminal region; The members of this family are are sequences that are similar to the N-terminal half of the ShET2 enterotoxin produced by Shigella flexneri and Escherichia coli. This protein was found to confer toxigenicity in the Ussing chamber, and the N-terminal region was found to be important for the protein's enterotoxic effect. It is thought to be a hydrophobic protein that forms inclusion bodies within the bacterial cell, and may be secreted by the Mxi system. Most members of this family are annotated as putative enterotoxins, but one member is a regulator of acetyl CoA synthetase, and another two members (Swiss:P76205 and Swiss:P23325) are annotated as ankyrin-like regulatory proteins and contain Ank repeats (pfam00023).


Pssm-ID: 369588  Cd Length: 278  Bit Score: 146.19  E-value: 6.28e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109    21 PYLSKKRDASINLNGKVSDCNGEIIWCRHIASYW-SEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGT----NNIKGDV 95
Cdd:pfam07906  22 PYFSEKKSFRINLNGQAYDRSGRLIVCRHLASQYiMDFLDEAKGKVDYKHFSSPDKIANHVSLEKKEQTyalfSSRPEGI 101
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109    96 YFVENESWGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKEN-KFVINFYDPNQTATHKRVFFctNNICDII 174
Cdd:pfam07906 102 YFIENGDFGLVLKAIFDKMESNGENVRALILLSSNHAMAVRLRIKETEEGcRYVVSVYDPNVTNTTVRCEV--DSLEKIK 179
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109   175 NLTAYDFLSEQ-------CLKCYGLKEDTLSLFVD--KTKSNDNNNVFIKKLPDNI--LQGVVINFAMGAGLREIIKKVy 243
Cdd:pfam07906 180 DYSLKDFINVDysllkysNDEIYGLISQGQAIIPDrhKPQLLSLKGSVDEISPLIKlpLSPAVLYLLMTIGFTEQIQQL- 258
                         250       260
                  ....*....|....*....|...
gi 15833109   244 ndtrftdltKSQMKILCESKNVN 266
Cdd:pfam07906 259 ---------MEQLQNISESHLTE 272
C58_PaToxP-like cd20495
peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large ...
113-155 5.60e-04

peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large cytotoxin, and similar proteins; This subfamily includes the cysteine protease domain of Photorhabdus asymbiotica toxin PaTox, a large virulence-associated multifunctional protein toxin. This domain is similar to AvrPphB protease found in Pseudomonas syringae, a C58 protease. Mutation studies show that this domain enhances cytotoxic effects of the toxin, and therefore is essential for full PaTox activity. Also included in this family is the enteropathogenic Escherichia coli (EPEC) factor for adherence/lymphocyte activation inhibitor (efa1/lifA) gene which is strongly associated with diarrhea. Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. This domain contains the invariant C/H/D residues conserved in the C58/YopT family.


Pssm-ID: 410776  Cd Length: 179  Bit Score: 41.19  E-value: 5.60e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 15833109 113 QLEKENKShTSLEVHSPGHAMALGIKIKNDKENKFvinFYDPN 155
Cdd:cd20495  94 ELANATTS-KTLLLNTPNHAMLAGVKVNGGKKSYF---FYDPN 132
 
Name Accession Description Interval E-value
Toxin_15 pfam07906
ShET2 enterotoxin, N-terminal region; The members of this family are are sequences that are ...
21-266 6.28e-40

ShET2 enterotoxin, N-terminal region; The members of this family are are sequences that are similar to the N-terminal half of the ShET2 enterotoxin produced by Shigella flexneri and Escherichia coli. This protein was found to confer toxigenicity in the Ussing chamber, and the N-terminal region was found to be important for the protein's enterotoxic effect. It is thought to be a hydrophobic protein that forms inclusion bodies within the bacterial cell, and may be secreted by the Mxi system. Most members of this family are annotated as putative enterotoxins, but one member is a regulator of acetyl CoA synthetase, and another two members (Swiss:P76205 and Swiss:P23325) are annotated as ankyrin-like regulatory proteins and contain Ank repeats (pfam00023).


Pssm-ID: 369588  Cd Length: 278  Bit Score: 146.19  E-value: 6.28e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109    21 PYLSKKRDASINLNGKVSDCNGEIIWCRHIASYW-SEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGT----NNIKGDV 95
Cdd:pfam07906  22 PYFSEKKSFRINLNGQAYDRSGRLIVCRHLASQYiMDFLDEAKGKVDYKHFSSPDKIANHVSLEKKEQTyalfSSRPEGI 101
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109    96 YFVENESWGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKEN-KFVINFYDPNQTATHKRVFFctNNICDII 174
Cdd:pfam07906 102 YFIENGDFGLVLKAIFDKMESNGENVRALILLSSNHAMAVRLRIKETEEGcRYVVSVYDPNVTNTTVRCEV--DSLEKIK 179
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15833109   175 NLTAYDFLSEQ-------CLKCYGLKEDTLSLFVD--KTKSNDNNNVFIKKLPDNI--LQGVVINFAMGAGLREIIKKVy 243
Cdd:pfam07906 180 DYSLKDFINVDysllkysNDEIYGLISQGQAIIPDrhKPQLLSLKGSVDEISPLIKlpLSPAVLYLLMTIGFTEQIQQL- 258
                         250       260
                  ....*....|....*....|...
gi 15833109   244 ndtrftdltKSQMKILCESKNVN 266
Cdd:pfam07906 259 ---------MEQLQNISESHLTE 272
C58_PaToxP-like cd20495
peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large ...
113-155 5.60e-04

peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large cytotoxin, and similar proteins; This subfamily includes the cysteine protease domain of Photorhabdus asymbiotica toxin PaTox, a large virulence-associated multifunctional protein toxin. This domain is similar to AvrPphB protease found in Pseudomonas syringae, a C58 protease. Mutation studies show that this domain enhances cytotoxic effects of the toxin, and therefore is essential for full PaTox activity. Also included in this family is the enteropathogenic Escherichia coli (EPEC) factor for adherence/lymphocyte activation inhibitor (efa1/lifA) gene which is strongly associated with diarrhea. Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. This domain contains the invariant C/H/D residues conserved in the C58/YopT family.


Pssm-ID: 410776  Cd Length: 179  Bit Score: 41.19  E-value: 5.60e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 15833109 113 QLEKENKShTSLEVHSPGHAMALGIKIKNDKENKFvinFYDPN 155
Cdd:cd20495  94 ELANATTS-KTLLLNTPNHAMLAGVKVNGGKKSYF---FYDPN 132
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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