|
Name |
Accession |
Description |
Interval |
E-value |
| PFL1 |
cd01678 |
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ... |
11-756 |
0e+00 |
|
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain] Cd Length: 738 Bit Score: 1504.96 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 11 AWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYI 90
Cdd:cd01678 1 AWEGFKGGKWQEEIDVRDFIQKNYTPYEGDESFLAGPTERTKKLWDKLEELLEEERAKGGVLDVDTKTVSTITSHKAGYI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 91 NKQLEKIVGLQTEAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLP 170
Cdd:cd01678 81 DKELEVIVGLQTDKPLKRAIMPFGGIRMAEQALKAYGYELDPELKKIFTKYRKTHNDGVFDAYTPEIRRARHSGIITGLP 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 171 DAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLEngvnLEQTIRLREEIAEQHRALGQMKEMAAKYGYDISGPAT 250
Cdd:cd01678 161 DAYGRGRIIGDYRRVALYGVDRLIEEKKKDLDNLGGDEM----TDDTIRLREEVAEQIKALKELKQMAASYGLDISRPAT 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 251 NAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:cd01678 237 NAQEAIQWTYFGYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKAGTITEAEAQELIDQFIMKLRMVRFLRTPEYNELFSG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 331 DPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTSSLQYENDDLMRPDF 410
Cdd:cd01678 317 DPTWVTESIGGMGNDGRSLVTKTSFRFLNTLYNLGPAPEPNLTVLWSEKLPENFKRFCAKVSIDTSSIQYENDDLMRPDW 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 411 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQ 490
Cdd:cd01678 397 GGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGRDEKTGDQVGPDIEPITSDYLDYDEVMENYDKSMDWLADT 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 491 YITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQFGNN 570
Cdd:cd01678 477 YVNALNIIHYMHDKYAYEALQMALHDTDVRRTMAFGIAGLSVAADSLSAIKYAKVKPIRDEDGLAVDFEIEGDFPRYGND 556
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 571 DPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:cd01678 557 DDRADDIAVWVVKTFMNKLRKHKTYRNAEPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDKKGALASLA 636
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHeasieGGQHLNVNVMNREMLLDAMENPEKYPQLT 730
Cdd:cd01678 637 SVAKLPYRDANDGISNTFSIVPNALGKTDEERIDNLVGILDGYFTK-----GGHHLNVNVLNRETLLDAMEHPEKYPQLT 711
|
730 740
....*....|....*....|....*.
gi 16128870 731 IRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:cd01678 712 IRVSGYAVNFVKLTREQQLDVISRTF 737
|
|
| pyr_form_ly_1 |
TIGR01255 |
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate ... |
11-760 |
0e+00 |
|
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate C-acetyltransferase This enzyme converts formate + acetyl-CoA into pyruvate + CoA. This model describes formate acetyltransferase 1. More distantly related putative formate acetyltransferases have also been identified, including formate acetyltransferase 2 from E. coli, which is excluded from this model. [Energy metabolism, Fermentation]
Pssm-ID: 273525 [Multi-domain] Cd Length: 744 Bit Score: 1440.49 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 11 AWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATEATTTLWDKVMEgVKLENRTHAPVDFDTAVASTITSHDAGYI 90
Cdd:TIGR01255 1 AWEGFTKGDWQNEVNVRDFIQKNYKPYEGDESFLAGPTEATTKVWDKVME-VKLENRTHAPVDFDTAVASTITSHDAGYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 91 NKQLEKIVGLQTEAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLP 170
Cdd:TIGR01255 80 DKQLEKIVGLQTEAPLKRALIPFGGIRMAEGSLKEYGRELDPMIHKIFTEYRKTHNQGVFDAYTPDIRRARKAGVLTGLP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 171 DAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENgvnleQTIRLREEIAEQHRALGQMKEMAAKYGYDISGPAT 250
Cdd:TIGR01255 160 DAYGRGRIIGDYRRVALYGIDYLMKEKLKQFTSLQADLEN-----ELIRLREEIAEQHRALGEMKEMAAKYGYDISRPAT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 251 NAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:TIGR01255 235 NAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDLKAGKITEQEAQEMVDHFVMKLRMVRFLRTPEYDELFSG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 331 DPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTSSLQYENDDLMRPDF 410
Cdd:TIGR01255 315 DPTWATESIAGMGLDGRTLVTKNSFRFLNTLYTMGPAPEPNMTVLWSEKLPLSFKKFAAKMSIDTSSIQYENDDLMRPDF 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 411 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQ 490
Cdd:TIGR01255 395 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVVPDIEPIKDEVLDYDEVMENMDKFLDWLAKQ 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 491 YITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQFGNN 570
Cdd:TIGR01255 475 YVTAMNIIHYMHDKYSYEASQMALHDTKVIRTMAFGIAGFSVAADSLSAIKYAKVKPIRDENGLAIDFEIEGDFPQYGND 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 571 DPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:TIGR01255 555 DDRVDDIAVDLVERFMKKLQKHHTYRNAIPTQSVLTITSNVVYGKKTGNTPDGRRVGAPFGPGANPMHGRDQKGALASLT 634
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLT 730
Cdd:TIGR01255 635 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKTNLVGIMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLT 714
|
730 740 750
....*....|....*....|....*....|
gi 16128870 731 IRVSGYAVRFNSLTKEQQQDVITRTFTQSM 760
Cdd:TIGR01255 715 IRVSGYAVNFNSLTKEQQQEVITRTFHESL 744
|
|
| PflD |
COG1882 |
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ... |
41-756 |
0e+00 |
|
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain] Cd Length: 789 Bit Score: 1042.43 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 41 ESFLAGATEATTTLWDKVMEGVKL--------------ENRTHAPVDFDTAVASTITSHDAGYInKQLEKIVGLQTEAPL 106
Cdd:COG1882 1 ESFLAGPTERTKRLREKLLEAKPLidierarlftesykETEGLPVIIRRAKAFSHILEHKPIYI-KDDELIVGLQTDKPR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 107 KRALIPFGGIKMIEGSCKAYNR------ELDPMIKKIFTEY-----RKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGR 175
Cdd:COG1882 80 KRPIFPEGGIRWVEDELDALPTrpqdgfEISPEDKEIFREIapywkGKTHNDGVFDAYPEEIRKARKAGIITGLPDAYGR 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 176 GRIIGDYRRVALYGIDYLMKDKLAQFTSLqaDLENGVNLEQ-------------TIRLREEIAEQHRAL----------G 232
Cdd:COG1882 160 GHIIGDYRRVLLYGLDGLIEEAKEKLAEL--DLTDPEDIEKidfykamiivceaVIRLAERYAELARELaeketdpkrkA 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 233 QMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMK 312
Cdd:COG1882 238 ELLEIAEICGFVPANPARTFWEAVQWVWFVYLAAIKEQNGAAMSLGRFDQYLYPYYERDLEEGRLTEEEAQELLDCFWIK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 313 LRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMgPSPEPNMTILWSEKLPLNFKKFAAK-V 391
Cdd:COG1882 318 LREVRFLRTPEYAELFAGYPTWVTLTIGGMTPDGRDAVNELSYLILETLRNL-PLPEPNLTVRWSEKLPEGFLKKAAEvI 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 392 SIDTSSLQYENDDLMRPDFNN--------DDYAIACCVSPMIVGKQMQFFGA-RANLAKTMLYAINGGVDEKLKMQVGPK 462
Cdd:COG1882 397 SIGTGSPQYENDDLMIPMLLNkgvtledaRDYGIAGCVEPMVPGKQMQFFGAgRINLAKALEYALNNGVDEKTGKQVGPE 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 463 SEPIKgDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYEASLMALHDRDVIRTM------------ACGIAGL 530
Cdd:COG1882 477 TGDPT-DFLTYDEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKdlneggarynfgAIGIAGL 555
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 531 SVAADSLSAIK---YAKVKPIRDE--DGLAIDFE-------IEGEYPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDA 598
Cdd:COG1882 556 SVVADSLSAIKklvFDKKKVTMDEllEALAANFEgyeelrqLLLNAPKYGNDDDYVDEIAVELVETFMDEIRKYKTYRGG 635
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 599 IPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKD 678
Cdd:COG1882 636 TYTLSILTITSNVPYGKKTGATPDGRKAGEPLADGASPMHGRDKNGPTAVLKSVAKLPYEKATDGILLNQKFSPSALGGE 715
|
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 16128870 679 DevRKTNLAGLMDGYFHHeasieGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:COG1882 716 E--GIENLVSLLRTYFDL-----GGHHIQFNVVDRETLLDAQKHPEKYPDLTVRVAGYSAYFVELSKEQQDDIIARTE 786
|
|
| PFL-like |
pfam02901 |
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ... |
30-614 |
0e+00 |
|
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048 Cd Length: 647 Bit Score: 755.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 30 IQKNYTPYEGDESFLagateatttlWDKVMEGVKLENRTHAPVDFDTAVAST---ITSHDAGYInKQLEKIVGLQTEAPL 106
Cdd:pfam02901 8 LKENYTLYTGDPSLS----------WERARLLTESYKETEGVLPVDIRRAKAlkkILSHLPGYI-RDDELIVGLQTDKPR 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 107 KRALIPFGGIKMIE------GSCKAYNRELDPMIKKIFTE-----YRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGR 175
Cdd:pfam02901 77 KRAIYPEGGIRWVEdeldylNTRPQDGFEISEEDKKIFREifpywKGKTHNEGVFDAYTPEMKAARESGIFTGLPDAYGR 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 176 GRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLEN---------------GVNL--EQTIRLREEIAEQH---RALGQMK 235
Cdd:pfam02901 157 GHIIGDYRRVLLYGLDGLIEEKEEKLAKLDTDPEDiekiefykamiiscdAVIEyaERYARLAEELAEQEtdpKRKAELL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 236 EMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRM 315
Cdd:pfam02901 237 EIAEICGRVPARPAETFQEAIQWFWFVYLAAVKEQNGAAMSLGRLDQYLYPYYERDLEEGRLTEEEAQELIDCFWIKLRE 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 316 VRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMgPSPEPNMTILWSEKLPLNFKKFAAKVSID- 394
Cdd:pfam02901 317 VRFLRTPEYNKLFAGYDPFQNLTIGGQGRDGRDAVNKLSYLILEALDNL-PLPEPNLTVRWSKKLPEEFLKKAAEVSRKg 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 395 TSSLQYENDDLMRPDFNND--------DYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPI 466
Cdd:pfam02901 396 TGSPQYENDDVMIPALLNRgvsledarDYGIAGCVEPMKPGKEMQFFGARINLAKALEYALNGGRDELTGKQVGPKTGPV 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 467 KgDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGI------------AGLSVAA 534
Cdd:pfam02901 476 T-EFLSFEEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDVGIggarynfsgpqgAGLANVA 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 535 DSLSAIKY----AKVKPIRD-EDGLAIDFEIEGE--------YPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPT 601
Cdd:pfam02901 555 DSLSAIKKlvfdDKVYTLRElEDALAADFEGEEElrqdllndAPKYGNDDDRVDDIAVEVVETFMDEVRKYKNYRGGKFT 634
|
650
....*....|...
gi 16128870 602 QSVLTITSNVVYG 614
Cdd:pfam02901 635 PSLLTITSNVPYG 647
|
|
| pflD |
PRK09983 |
putative formate acetyltransferase 2; Provisional |
174-758 |
3.03e-43 |
|
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain] Cd Length: 765 Bit Score: 167.69 E-value: 3.03e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 174 GRGRIIGDYRRVALYGIDYL---MKDKLAQFTS---LQADLengVNLEQTIR-------LREEIAE-----QHRAlgQMK 235
Cdd:PRK09983 153 GQGHIIIDYPRLLNHGLGELvaqMQQHCQQQPEnhfYQAAL---LLLEASQKhilryaeLAETMAAnctdaQRRE--ELL 227
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 236 EMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGkITEQEAQEMVDHLVMKLRM 315
Cdd:PRK09983 228 TIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQG-EDPAFLKELLESLWVKCND 306
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 316 VRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTlYTMGPSPEPNMTILWSEKLPLNF-KKFAAKVSID 394
Cdd:PRK09983 307 IVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDA-YQSVQLPQPNLGVRTNALIDTPFlMKTAETIRLG 385
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 395 TSSLQYENDDLMRPDFNND--------DYAIACCVSPMIVGKQmqfFG----ARANLAKTM---LYAINGGvdeklkmqv 459
Cdd:PRK09983 386 TGIPQIFNDEVVVPAFLNRgvsledarDYSVVGCVELSIPGRT---YGlhdiAMFNLLKVMeicLHENEGN--------- 453
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 460 gpksepikgDVLNYDEVMermDHFMDWLaKQYITAL----NIIHYMHDKYSYEASLMAL------HDRDVI----RTMAC 525
Cdd:PRK09983 454 ---------AALTYEGLL---EQIRAKI-SHYITLMvegsNICDIGHRDWAPVPLLSSFisdcleKGRDITdggaRYNFS 520
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 526 GIAGLSVA--ADSLSAIKyakvKPIRDEDGLAIDFEI----------EGE---------YPQFGNNDPRVDDLAVDLVER 584
Cdd:PRK09983 521 GVQGIGIAnlSDSLHALK----GMVFDQQRLSFDELLsvlkanfatpEGEkvrarlinrFEKYGNDIDEVDNISAELLRH 596
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 585 FMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGA-NPMHGRDQKGAVASLTSVAKLPFAYAKDG 663
Cdd:PRK09983 597 YCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGlSPMLGQDAQGPTAVLKSVSKLDNTLLSNG 676
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 664 ISYTFSIVPNALGKDDEVRKtnLAGLMDGYFHHEAsieggQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSL 743
Cdd:PRK09983 677 TLLNVKFTPATLEGEAGLRK--LADFLRAFTQLKL-----QHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVEL 749
|
650
....*....|....*
gi 16128870 744 TKEQQQDVITRTFTQ 758
Cdd:PRK09983 750 SKEIQDDIIRRTAHQ 764
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PFL1 |
cd01678 |
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, ... |
11-756 |
0e+00 |
|
Pyruvate formate lyase 1; Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. The PFL mechanism involves an unusual radical cleavage of pyruvate in which two cysteines and one glycine form radicals that are required for catalysis. PFL has a ten-stranded alpha/beta barrel domain that is structurally similar to those of all three ribonucleotide reductase (RNR) classes as well as benzylsuccinate synthase and B12-independent glycerol dehydratase.
Pssm-ID: 153087 [Multi-domain] Cd Length: 738 Bit Score: 1504.96 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 11 AWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYI 90
Cdd:cd01678 1 AWEGFKGGKWQEEIDVRDFIQKNYTPYEGDESFLAGPTERTKKLWDKLEELLEEERAKGGVLDVDTKTVSTITSHKAGYI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 91 NKQLEKIVGLQTEAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLP 170
Cdd:cd01678 81 DKELEVIVGLQTDKPLKRAIMPFGGIRMAEQALKAYGYELDPELKKIFTKYRKTHNDGVFDAYTPEIRRARHSGIITGLP 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 171 DAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLEngvnLEQTIRLREEIAEQHRALGQMKEMAAKYGYDISGPAT 250
Cdd:cd01678 161 DAYGRGRIIGDYRRVALYGVDRLIEEKKKDLDNLGGDEM----TDDTIRLREEVAEQIKALKELKQMAASYGLDISRPAT 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 251 NAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:cd01678 237 NAQEAIQWTYFGYLAAIKEQNGAAMSLGRVSTFLDIYIERDLKAGTITEAEAQELIDQFIMKLRMVRFLRTPEYNELFSG 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 331 DPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTSSLQYENDDLMRPDF 410
Cdd:cd01678 317 DPTWVTESIGGMGNDGRSLVTKTSFRFLNTLYNLGPAPEPNLTVLWSEKLPENFKRFCAKVSIDTSSIQYENDDLMRPDW 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 411 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQ 490
Cdd:cd01678 397 GGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYAINGGRDEKTGDQVGPDIEPITSDYLDYDEVMENYDKSMDWLADT 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 491 YITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQFGNN 570
Cdd:cd01678 477 YVNALNIIHYMHDKYAYEALQMALHDTDVRRTMAFGIAGLSVAADSLSAIKYAKVKPIRDEDGLAVDFEIEGDFPRYGND 556
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 571 DPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:cd01678 557 DDRADDIAVWVVKTFMNKLRKHKTYRNAEPTQSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDKKGALASLA 636
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHeasieGGQHLNVNVMNREMLLDAMENPEKYPQLT 730
Cdd:cd01678 637 SVAKLPYRDANDGISNTFSIVPNALGKTDEERIDNLVGILDGYFTK-----GGHHLNVNVLNRETLLDAMEHPEKYPQLT 711
|
730 740
....*....|....*....|....*.
gi 16128870 731 IRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:cd01678 712 IRVSGYAVNFVKLTREQQLDVISRTF 737
|
|
| pyr_form_ly_1 |
TIGR01255 |
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate ... |
11-760 |
0e+00 |
|
formate acetyltransferase 1; Alternate names: pyruvate formate-lyase; formate C-acetyltransferase This enzyme converts formate + acetyl-CoA into pyruvate + CoA. This model describes formate acetyltransferase 1. More distantly related putative formate acetyltransferases have also been identified, including formate acetyltransferase 2 from E. coli, which is excluded from this model. [Energy metabolism, Fermentation]
Pssm-ID: 273525 [Multi-domain] Cd Length: 744 Bit Score: 1440.49 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 11 AWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATEATTTLWDKVMEgVKLENRTHAPVDFDTAVASTITSHDAGYI 90
Cdd:TIGR01255 1 AWEGFTKGDWQNEVNVRDFIQKNYKPYEGDESFLAGPTEATTKVWDKVME-VKLENRTHAPVDFDTAVASTITSHDAGYI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 91 NKQLEKIVGLQTEAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLP 170
Cdd:TIGR01255 80 DKQLEKIVGLQTEAPLKRALIPFGGIRMAEGSLKEYGRELDPMIHKIFTEYRKTHNQGVFDAYTPDIRRARKAGVLTGLP 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 171 DAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENgvnleQTIRLREEIAEQHRALGQMKEMAAKYGYDISGPAT 250
Cdd:TIGR01255 160 DAYGRGRIIGDYRRVALYGIDYLMKEKLKQFTSLQADLEN-----ELIRLREEIAEQHRALGEMKEMAAKYGYDISRPAT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 251 NAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSG 330
Cdd:TIGR01255 235 NAKEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIERDLKAGKITEQEAQEMVDHFVMKLRMVRFLRTPEYDELFSG 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 331 DPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTSSLQYENDDLMRPDF 410
Cdd:TIGR01255 315 DPTWATESIAGMGLDGRTLVTKNSFRFLNTLYTMGPAPEPNMTVLWSEKLPLSFKKFAAKMSIDTSSIQYENDDLMRPDF 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 411 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQ 490
Cdd:TIGR01255 395 NNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVVPDIEPIKDEVLDYDEVMENMDKFLDWLAKQ 474
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 491 YITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQFGNN 570
Cdd:TIGR01255 475 YVTAMNIIHYMHDKYSYEASQMALHDTKVIRTMAFGIAGFSVAADSLSAIKYAKVKPIRDENGLAIDFEIEGDFPQYGND 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 571 DPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLT 650
Cdd:TIGR01255 555 DDRVDDIAVDLVERFMKKLQKHHTYRNAIPTQSVLTITSNVVYGKKTGNTPDGRRVGAPFGPGANPMHGRDQKGALASLT 634
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 651 SVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLT 730
Cdd:TIGR01255 635 SVAKLPFAYAKDGISYTFSIVPNALGKDDDVRKTNLVGIMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLT 714
|
730 740 750
....*....|....*....|....*....|
gi 16128870 731 IRVSGYAVRFNSLTKEQQQDVITRTFTQSM 760
Cdd:TIGR01255 715 IRVSGYAVNFNSLTKEQQQEVITRTFHESL 744
|
|
| PflD |
COG1882 |
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of ... |
41-756 |
0e+00 |
|
Pyruvate-formate lyase [Energy production and conversion]; Pyruvate-formate lyase is part of the Pathway/BioSystem: Pyruvate oxidation
Pssm-ID: 441486 [Multi-domain] Cd Length: 789 Bit Score: 1042.43 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 41 ESFLAGATEATTTLWDKVMEGVKL--------------ENRTHAPVDFDTAVASTITSHDAGYInKQLEKIVGLQTEAPL 106
Cdd:COG1882 1 ESFLAGPTERTKRLREKLLEAKPLidierarlftesykETEGLPVIIRRAKAFSHILEHKPIYI-KDDELIVGLQTDKPR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 107 KRALIPFGGIKMIEGSCKAYNR------ELDPMIKKIFTEY-----RKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGR 175
Cdd:COG1882 80 KRPIFPEGGIRWVEDELDALPTrpqdgfEISPEDKEIFREIapywkGKTHNDGVFDAYPEEIRKARKAGIITGLPDAYGR 159
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 176 GRIIGDYRRVALYGIDYLMKDKLAQFTSLqaDLENGVNLEQ-------------TIRLREEIAEQHRAL----------G 232
Cdd:COG1882 160 GHIIGDYRRVLLYGLDGLIEEAKEKLAEL--DLTDPEDIEKidfykamiivceaVIRLAERYAELARELaeketdpkrkA 237
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 233 QMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMK 312
Cdd:COG1882 238 ELLEIAEICGFVPANPARTFWEAVQWVWFVYLAAIKEQNGAAMSLGRFDQYLYPYYERDLEEGRLTEEEAQELLDCFWIK 317
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 313 LRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMgPSPEPNMTILWSEKLPLNFKKFAAK-V 391
Cdd:COG1882 318 LREVRFLRTPEYAELFAGYPTWVTLTIGGMTPDGRDAVNELSYLILETLRNL-PLPEPNLTVRWSEKLPEGFLKKAAEvI 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 392 SIDTSSLQYENDDLMRPDFNN--------DDYAIACCVSPMIVGKQMQFFGA-RANLAKTMLYAINGGVDEKLKMQVGPK 462
Cdd:COG1882 397 SIGTGSPQYENDDLMIPMLLNkgvtledaRDYGIAGCVEPMVPGKQMQFFGAgRINLAKALEYALNNGVDEKTGKQVGPE 476
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 463 SEPIKgDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYEASLMALHDRDVIRTM------------ACGIAGL 530
Cdd:COG1882 477 TGDPT-DFLTYDEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKdlneggarynfgAIGIAGL 555
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 531 SVAADSLSAIK---YAKVKPIRDE--DGLAIDFE-------IEGEYPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDA 598
Cdd:COG1882 556 SVVADSLSAIKklvFDKKKVTMDEllEALAANFEgyeelrqLLLNAPKYGNDDDYVDEIAVELVETFMDEIRKYKTYRGG 635
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 599 IPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKD 678
Cdd:COG1882 636 TYTLSILTITSNVPYGKKTGATPDGRKAGEPLADGASPMHGRDKNGPTAVLKSVAKLPYEKATDGILLNQKFSPSALGGE 715
|
730 740 750 760 770 780 790
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 16128870 679 DevRKTNLAGLMDGYFHHeasieGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTF 756
Cdd:COG1882 716 E--GIENLVSLLRTYFDL-----GGHHIQFNVVDRETLLDAQKHPEKYPDLTVRVAGYSAYFVELSKEQQDDIIARTE 786
|
|
| PFL-like |
pfam02901 |
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline ... |
30-614 |
0e+00 |
|
Pyruvate formate lyase-like; This family of enzymes includes pyruvate formate lyase, choline trimethylamine lyase, glycerol dehydratase, 4-hydroxyphenylacetate decarboxylase, and benzylsuccinate synthase.
Pssm-ID: 427048 Cd Length: 647 Bit Score: 755.62 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 30 IQKNYTPYEGDESFLagateatttlWDKVMEGVKLENRTHAPVDFDTAVAST---ITSHDAGYInKQLEKIVGLQTEAPL 106
Cdd:pfam02901 8 LKENYTLYTGDPSLS----------WERARLLTESYKETEGVLPVDIRRAKAlkkILSHLPGYI-RDDELIVGLQTDKPR 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 107 KRALIPFGGIKMIE------GSCKAYNRELDPMIKKIFTE-----YRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGR 175
Cdd:pfam02901 77 KRAIYPEGGIRWVEdeldylNTRPQDGFEISEEDKKIFREifpywKGKTHNEGVFDAYTPEMKAARESGIFTGLPDAYGR 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 176 GRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLEN---------------GVNL--EQTIRLREEIAEQH---RALGQMK 235
Cdd:pfam02901 157 GHIIGDYRRVLLYGLDGLIEEKEEKLAKLDTDPEDiekiefykamiiscdAVIEyaERYARLAEELAEQEtdpKRKAELL 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 236 EMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRM 315
Cdd:pfam02901 237 EIAEICGRVPARPAETFQEAIQWFWFVYLAAVKEQNGAAMSLGRLDQYLYPYYERDLEEGRLTEEEAQELIDCFWIKLRE 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 316 VRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMgPSPEPNMTILWSEKLPLNFKKFAAKVSID- 394
Cdd:pfam02901 317 VRFLRTPEYNKLFAGYDPFQNLTIGGQGRDGRDAVNKLSYLILEALDNL-PLPEPNLTVRWSKKLPEEFLKKAAEVSRKg 395
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 395 TSSLQYENDDLMRPDFNND--------DYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPI 466
Cdd:pfam02901 396 TGSPQYENDDVMIPALLNRgvsledarDYGIAGCVEPMKPGKEMQFFGARINLAKALEYALNGGRDELTGKQVGPKTGPV 475
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 467 KgDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGI------------AGLSVAA 534
Cdd:pfam02901 476 T-EFLSFEEVMEAFKKQLDYLADLYVNALNIIHYMHDKYAPEPFLSALHDDCIERGKDVGIggarynfsgpqgAGLANVA 554
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 535 DSLSAIKY----AKVKPIRD-EDGLAIDFEIEGE--------YPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPT 601
Cdd:pfam02901 555 DSLSAIKKlvfdDKVYTLRElEDALAADFEGEEElrqdllndAPKYGNDDDRVDDIAVEVVETFMDEVRKYKNYRGGKFT 634
|
650
....*....|...
gi 16128870 602 QSVLTITSNVVYG 614
Cdd:pfam02901 635 PSLLTITSNVPYG 647
|
|
| RNR_PFL |
cd00576 |
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and ... |
154-710 |
2.06e-97 |
|
Ribonucleotide reductase and Pyruvate formate lyase; Ribonucleotide reductase (RNR) and pyruvate formate lyase (PFL) are believed to have diverged from a common ancestor. They have a structurally similar ten-stranded alpha-beta barrel domain that hosts the active site, and are radical enzymes. RNRs are found in all organisms and provide the only mechanism by which nucleotides are converted to deoxynucleotides. RNRs are separated into three classes based on their metallocofactor usage. Class I RNRs use a diiron-tyrosyl radical while Class II RNRs use coenzyme B12 (adenosylcobalamin, AdoCbl). Class III RNRs use an FeS cluster and S-adenosylmethionine to generate a glycyl radical. PFL, an essential enzyme in anaerobic bacteria, catalyzes the conversion of pyruvate and CoA to acteylCoA and formate in a mechanism that uses a glycyl radical.
Pssm-ID: 153083 [Multi-domain] Cd Length: 401 Bit Score: 307.54 E-value: 2.06e-97
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 154 TPDILRCRKSGVLTGLPDAY-GRGRIIGDYRRVALYGIDYLMkdklaqftslqadlengvnleqtirlreeiaeqhralg 232
Cdd:cd00576 1 AERIYEAVKSGVITVGRPDLpFTGCVLVDYGDSLDPGIKGVN-------------------------------------- 42
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 233 qmkemaakygydisGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMK 312
Cdd:cd00576 43 --------------ETAKSINEAIQKTYQIIALAASNQNGGGVSFARASSILSPYGSRDYAKGSGTETDAVEAADAFNLA 108
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 313 LRMVRFLrtpeydelfSGDPIWATESIGGMGLDgrtLVTKNSFRFLNTLYTMG----PSPEPNMTILWSEKLPL------ 382
Cdd:cd00576 109 LKEVGQG---------NGRTGAATGFIGGVHKG---KGDKISQEFLNLALANGgegiPLNFPNLSVRVSSDKPGilvkav 176
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 383 NFKKFAAKVSIDTSSLQYENDdlmrpdfnnddyaIACcvspmivgkqmqfFGARANLAKTMLYAINGgvdeklkmqvgpk 462
Cdd:cd00576 177 ELKQLIAEEARKTGSPGIFND-------------ELC-------------NLVSLNLARIMEKAING------------- 217
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 463 sepikgdvlnydeVMERMDHFMDWLAKQYITALNIIHYMHDKYSYEaslmALHDRDVIRTMACGIAGLSVAADSLSAIKY 542
Cdd:cd00576 218 -------------SMDVVLEELEELAFLAVRALDCVIDSHDERIPT----IELGGDERRTVGLGIAGVADLLIKLGLEKV 280
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 543 AkvkpirdedglaidfeiegeypqfgnnDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNvvygkktgntpd 622
Cdd:cd00576 281 G---------------------------DPEADDLAAELVDQLKKHLVKATNERGFNFLLGLSPSESN------------ 321
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 623 grRAGAPFGPGANPMHGR------DQKGAVASLTSVAKLPFaYAKDGISYTFSIVPNALGKDdevrktNLAGLMDGYFHH 696
Cdd:cd00576 322 --SSGAPATNGVSPSRG*iaivlnGDIGPEESLASVAILQF-YADNGISDTITIPDSATNLD------QLLAVIDGAAAI 392
|
570
....*....|....
gi 16128870 697 easieGGQHLNVNV 710
Cdd:cd00576 393 -----KTTHVRVNP 401
|
|
| PFL2_DhaB_BssA |
cd01677 |
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 ... |
95-755 |
2.19e-81 |
|
Pyruvate formate lyase 2 and related enzymes; This family includes pyruvate formate lyase 2 (PFL2), B12-independent glycerol dehydratase (DhaB) and the alpha subunit of benzylsuccinate synthase (BssA), all of which have a highly conserved ten-stranded alpha/beta barrel domain, which is similar to those of PFL1 (pyruvate formate lyase 1) and RNR (ribonucleotide reductase). Pyruvate formate lyase catalyzes a key step in anaerobic glycolysis, the conversion of pyruvate and CoenzymeA to formate and acetylCoA. DhaB catalyzes the first step in the conversion of glycerol to 1,3-propanediol while BssA catalyzes the first step in the anaerobic mineralization of both toluene and m-xylene.
Pssm-ID: 153086 [Multi-domain] Cd Length: 781 Bit Score: 276.08 E-value: 2.19e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 95 EKIVGLQTEAPLKRALIPfggikmiEGSCKA--------YNRELDPMI----KKIFTE------YRKTHNQGVFDVYTPD 156
Cdd:cd01677 61 ELIVGNRGGKPRAVPVFP-------ELSVHWvedelddlPKRPGDPFVisedKKEYLEeifpywKGKTLRDRCFKYFPEE 133
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 157 ILRCRKSGVLTGLPDAY-GRGRIIGDYRRVALYGIDYLMKDklAQFTSLQADLENGVNLEQTIRLREEI---------AE 226
Cdd:cd01677 134 TLIAMAAGVFTEFMYFFsGPGHVAVDYPKVLEKGLDGLIEE--AKEAIEALDLTGPEDIDKIYFYQAMIivceavityAK 211
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 227 QHRALGqmKEMAAKYG--------YDISG--------PATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIER 290
Cdd:cd01677 212 RYAELA--KELAAKETdpkrkaelLEIAEicrrvpahPPRTFWEALQSFWFIHLILQIESNGHSISPGRFDQYLYPFYKQ 289
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 291 DLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTLYTMgPSPEP 370
Cdd:cd01677 290 DIEEGRLTREGAIELLECLWIKINEINKVRSGASAKYFAGYNTFQNLTIGGQTEDGSDATNELSYLILEATRRV-RLPQP 368
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 371 NMTILWSEKLPLNFKKFAAKVSIDTSSL-QYENDDLMRPDFNND--------DYAIACCVSPMIVGKQMQFFGARA-NLA 440
Cdd:cd01677 369 SLTVRYHAKSPDKFLKKAAEVIRLGLGYpAFFNDEVVIPALLRKgvsledarDYGLIGCVETGAPGRKYRWTGTGYiNLA 448
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 441 KTMLYAINGGVDEKLKMQVGPKSEPIKgDVLNYDEVME----RMDHFMDWLAKqyitALNIIHYMHDKYSYEASLMALHD 516
Cdd:cd01677 449 KVLEITLNNGKDPRSGKQVGPETGDAT-DFKTFEELWEafkkQLRHFIKLSVR----ANNISDIAHAEVAPAPFLSALVD 523
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 517 ------RDV---------IRTMACGIAglsVAADSLSAIKY-----AKVKPIRDEDGLAIDFEiEGE---------YPQF 567
Cdd:cd01677 524 dciekgKDInaggarynfGGIQGVGIA---TLGDSLAAIKKlvfeeKKLTMEELLEALKANFA-EGYeerrrllnnAPKY 599
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 568 GNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVA 647
Cdd:cd01677 600 GNDDDYADNIARRVYEWYCKEVEKYQNPRGGKFYPGTYSVSANVPFGSVTGATPDGRLAGTPLSDGVSPSQGTDKKGPTA 679
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 648 SLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKtnLAGLMDGYFhheasIEGGQHLNVNVMNREMLLDAMENPEKYP 727
Cdd:cd01677 680 VIKSVSKLDHFNISGGTLLNQKFSPSTLEGEEGLKK--LAALIRTYF-----DLGGHHIQFNVVSAETLRDAQKHPEKYR 752
|
730 740
....*....|....*....|....*...
gi 16128870 728 QLTIRVSGYAVRFNSLTKEQQQDVITRT 755
Cdd:cd01677 753 DLIVRVAGYSAYFVELSKEVQDEIIART 780
|
|
| Gly_radical |
pfam01228 |
Glycine radical; |
630-741 |
2.84e-50 |
|
Glycine radical;
Pssm-ID: 426140 [Multi-domain] Cd Length: 106 Bit Score: 171.20 E-value: 2.84e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 630 FGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHheasieGGQHLNVN 709
Cdd:pfam01228 1 VAPGISPSHGADFEGPTAVLNSVGKIDYEVELDGISLNQKFLPAVLGYYDEEGYANLNTLIDTYFE------GGHHLQFN 74
|
90 100 110
....*....|....*....|....*....|..
gi 16128870 710 VMNREMLLDAMENPEKYPQLTIRVSGYAVRFN 741
Cdd:pfam01228 75 VVDRETLPDAQKHPEKYPDLTVRVSGYSANFV 106
|
|
| pflD |
PRK09983 |
putative formate acetyltransferase 2; Provisional |
174-758 |
3.03e-43 |
|
putative formate acetyltransferase 2; Provisional
Pssm-ID: 182181 [Multi-domain] Cd Length: 765 Bit Score: 167.69 E-value: 3.03e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 174 GRGRIIGDYRRVALYGIDYL---MKDKLAQFTS---LQADLengVNLEQTIR-------LREEIAE-----QHRAlgQMK 235
Cdd:PRK09983 153 GQGHIIIDYPRLLNHGLGELvaqMQQHCQQQPEnhfYQAAL---LLLEASQKhilryaeLAETMAAnctdaQRRE--ELL 227
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 236 EMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGkITEQEAQEMVDHLVMKLRM 315
Cdd:PRK09983 228 TIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQG-EDPAFLKELLESLWVKCND 306
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 316 VRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNTlYTMGPSPEPNMTILWSEKLPLNF-KKFAAKVSID 394
Cdd:PRK09983 307 IVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDA-YQSVQLPQPNLGVRTNALIDTPFlMKTAETIRLG 385
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 395 TSSLQYENDDLMRPDFNND--------DYAIACCVSPMIVGKQmqfFG----ARANLAKTM---LYAINGGvdeklkmqv 459
Cdd:PRK09983 386 TGIPQIFNDEVVVPAFLNRgvsledarDYSVVGCVELSIPGRT---YGlhdiAMFNLLKVMeicLHENEGN--------- 453
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 460 gpksepikgDVLNYDEVMermDHFMDWLaKQYITAL----NIIHYMHDKYSYEASLMAL------HDRDVI----RTMAC 525
Cdd:PRK09983 454 ---------AALTYEGLL---EQIRAKI-SHYITLMvegsNICDIGHRDWAPVPLLSSFisdcleKGRDITdggaRYNFS 520
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 526 GIAGLSVA--ADSLSAIKyakvKPIRDEDGLAIDFEI----------EGE---------YPQFGNNDPRVDDLAVDLVER 584
Cdd:PRK09983 521 GVQGIGIAnlSDSLHALK----GMVFDQQRLSFDELLsvlkanfatpEGEkvrarlinrFEKYGNDIDEVDNISAELLRH 596
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 585 FMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGA-NPMHGRDQKGAVASLTSVAKLPFAYAKDG 663
Cdd:PRK09983 597 YCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGlSPMLGQDAQGPTAVLKSVSKLDNTLLSNG 676
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 664 ISYTFSIVPNALGKDDEVRKtnLAGLMDGYFHHEAsieggQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSL 743
Cdd:PRK09983 677 TLLNVKFTPATLEGEAGLRK--LADFLRAFTQLKL-----QHIQFNVVNADTLREAQQRPQDYAGLVVRVAGYSAFFVEL 749
|
650
....*....|....*
gi 16128870 744 TKEQQQDVITRTFTQ 758
Cdd:PRK09983 750 SKEIQDDIIRRTAHQ 764
|
|
| PRK11127 |
PRK11127 |
autonomous glycyl radical cofactor GrcA; Provisional |
697-760 |
1.82e-40 |
|
autonomous glycyl radical cofactor GrcA; Provisional
Pssm-ID: 182983 [Multi-domain] Cd Length: 127 Bit Score: 144.60 E-value: 1.82e-40
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 16128870 697 EASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSM 760
Cdd:PRK11127 64 EVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFTESL 127
|
|
| GrcA |
COG3445 |
Autonomous glycyl radical cofactor GrcA [Coenzyme transport and metabolism]; |
696-760 |
1.08e-39 |
|
Autonomous glycyl radical cofactor GrcA [Coenzyme transport and metabolism];
Pssm-ID: 442669 [Multi-domain] Cd Length: 125 Bit Score: 142.57 E-value: 1.08e-39
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 16128870 696 HEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSM 760
Cdd:COG3445 61 APPRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL 125
|
|
| spare_glycyl |
TIGR04365 |
autonomous glycyl radical cofactor GrcA; This small protein, previously designated YfiD in E. ... |
696-760 |
1.13e-38 |
|
autonomous glycyl radical cofactor GrcA; This small protein, previously designated YfiD in E. coli, is closely homologous to pyruvate formate_lyase (PFL) in a region surrounding the stable glycyl radical that is prepared by the action of pyruvate formate-lyase activase, a radical SAM enzyme. When damage at the site of this radical breaks the main chain of PFL, this protein acts as a spare part that reintroduces the needed stable glycyl radical. Cutoffs for this model are set to exclude a set of closely related phage proteins that appear to have a corresponding function.
Pssm-ID: 213978 [Multi-domain] Cd Length: 124 Bit Score: 139.55 E-value: 1.13e-38
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 16128870 696 HEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSM 760
Cdd:TIGR04365 60 APAKVEGGQHLNVNVLTRETLEDAVKNPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTESL 124
|
|
| choline_CutC |
TIGR04394 |
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and ... |
248-755 |
5.37e-34 |
|
choline trimethylamine-lyase; Members of this family, homologs to pyruvate formate-lyases and benzylsuccinate synthases, are glycine radical enzymes that appear to act as choline TMA-lyase, that is, to perform a C-N bond cleavage turning choline into trimethylamine (TMA) plus acetaldehyde. The gene symbol is cutC, for choline utilization. The activase, CutD, is a radical SAM enzyme. [Energy metabolism, Amino acids and amines]
Pssm-ID: 275187 [Multi-domain] Cd Length: 789 Bit Score: 139.55 E-value: 5.37e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 248 PATNAQEAIQ--WTYFGYLAAVKSQNGaaMSFGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYD 325
Cdd:TIGR04394 252 KPRTFWEAIQsvWTVESLLVVEENQTG--MSIGRVDQYMYPFYKADIEAGRMTEYEAFELAGCMLIKMSEMMWLTSEGGS 329
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 326 ELFSGDPIWATESIGGMGLDGR------TLVTKNSFRFLNtLYtmgpspEPNMTILWSEKLPlnfKKFAAK-VSIDTSSL 398
Cdd:TIGR04394 330 KFFAGYQPFVNMCVGGVTREGGdatndlTYLLMDAVRHVK-VY------QPSLACRIHNKSP---QKYLKKiVDVVRAGM 399
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 399 QYEN---DD-----LMRPDFNNDD---YAIACCVSPMIVGKQMQFFG-ARANLAKTMLYAINGGVDEKLKMQVGPKSepi 466
Cdd:TIGR04394 400 GFPAchfDDahikmMLAKGVSIEDardYCLMGCVEPQKSGRLYQWTStAYTQWPICIELVLNHGVPLWYGKQVCPDT--- 476
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 467 kGDVLNYDE-------VMERMDHFMDWLAKQYITALNIIHYMHDKysyeaSLMAL-------HDRDVIRTMAC-----GI 527
Cdd:TIGR04394 477 -GDLSQFDTyekfdaaVKEQIKYITKWSAVATVISQRVHRDLAPK-----PLMSLmyegcmeKGKDVSAGGAMynfgpGV 550
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 528 --AGLSVAADSLSAIK---YAKVKPIRDEDGLAIDFEIEG---------EYPQFGNNDPRVDDLAVDLV---ERFMKKIQ 590
Cdd:TIGR04394 551 vwSGLATYADSMAAIKklvYDDKKYTLEQLNEALKANFEGyeqiradclDAPKYGNDDDYADLIAADLVnftEREHRKYK 630
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 591 KLHTyrdaIPTQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSI 670
Cdd:TIGR04394 631 TLYS----HLSHGTLSISNNTPFGQLTGASANGRLAWTPLSDGISPTQGADFKGPTAIIKSVSKMANDSMNIGMVHNFKL 706
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16128870 671 VPNALgkDDEVRKTNLAGLMdgyfhHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQD 750
Cdd:TIGR04394 707 MSGLL--DTPEGENGLITLL-----RTASILGNGEMQFNYLDNETLLDAQQHPEKYRDLVVRVAGYSAFFVELCKDVQDE 779
|
....*
gi 16128870 751 VITRT 755
Cdd:TIGR04394 780 IISRT 784
|
|
| |
