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Conserved domains on  [gi|90111202|ref|NP_415527|]
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FMN reductase RutF [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

pyrimidine utilization flavin reductase protein F( domain architecture ID 10022704)

pyrimidine utilization flavin reductase protein F catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RutF TIGR03615
pyrimidine utilization flavin reductase protein F; This protein is observed in operons ...
 4-159 1.65e-106

pyrimidine utilization flavin reductase protein F; This protein is observed in operons extremely similar to that characterized in E. coli K-12 responsible for the import and catabolism of pyrimidines, primarily uracil. This protein is a member of the flavin reductase family defined by pfam01613. Presumably, this protein recycles the flavin of the RutA luciferase-like oxidoreductase.


:

Pssm-ID: 132654  Cd Length: 156  Bit Score: 300.95  E-value: 1.65e-106
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202     4 VDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSN 83
Cdd:TIGR03615   1 VDQQAFRDAMSRLGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASAYPAFKQNGTLCVNTLAAGQEDLSN 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 90111202    84 LFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHAL 159
Cdd:TIGR03615  81 LFGGKTPMEERFAAGQWRTLVTGAPVLEDALVSFDCRISQSHSVGTHDILFCAVEAIAHSAGADALVYFNRRYHTL 156
 
Name Accession Description Interval E-value
RutF TIGR03615
pyrimidine utilization flavin reductase protein F; This protein is observed in operons ...
 4-159 1.65e-106

pyrimidine utilization flavin reductase protein F; This protein is observed in operons extremely similar to that characterized in E. coli K-12 responsible for the import and catabolism of pyrimidines, primarily uracil. This protein is a member of the flavin reductase family defined by pfam01613. Presumably, this protein recycles the flavin of the RutA luciferase-like oxidoreductase.


Pssm-ID: 132654  Cd Length: 156  Bit Score: 300.95  E-value: 1.65e-106
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202     4 VDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSN 83
Cdd:TIGR03615   1 VDQQAFRDAMSRLGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASAYPAFKQNGTLCVNTLAAGQEDLSN 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 90111202    84 LFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHAL 159
Cdd:TIGR03615  81 LFGGKTPMEERFAAGQWRTLVTGAPVLEDALVSFDCRISQSHSVGTHDILFCAVEAIAHSAGADALVYFNRRYHTL 156
hpaC PRK15486
4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional
 4-160 1.72e-50

4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional


Pssm-ID: 185383  Cd Length: 170  Bit Score: 159.44  E-value: 1.72e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202    4 VDQQT--FRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPL 81
Cdd:PRK15486   3 LDEQRlrFRDAMASLSAAVNIVTTAGDAGRCGITATAVCSVTDTPPSVMVCINANSAMNPVFQGNGKLCINVLNHEQELM 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202   82 SNLFGGKT--PMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHAL 159
Cdd:PRK15486  83 ARHFAGMTgmAMEERFSLSCWQKGPLGQPVLKGSLASLEGEISDVQTIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPV 162


                 .
gi 90111202  160 M 160
Cdd:PRK15486 163 M 163
RutF COG1853
FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, ...
 5-158 1.10e-49

FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, DIM6/NTAB family is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 441458  Cd Length: 160  Bit Score: 156.91  E-value: 1.10e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202   5 DQQTFRDAMSCMGAAVNIITTDGPAG-RAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSN 83
Cdd:COG1853   1 MPDAFRDALGRLAPGVAVVTTRDADGrPNGMTASSFTSVSLDPPLVLVSVAKSSDTLENIRETGEFVVNVLSEDQAELAN 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202  84 LFGGKT-PMEHRFAAARWQT--GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYG--LVWFDRSYHA 158
Cdd:COG1853  81 RFAGRSgRGVDKFAGAGLTTasGEVGAPLLAEAPAWLECRVVDVIELGDHTLFIGEVVAVHVDEDVDGrpLLYLGGRYRR 160
Flavin_Reduct smart00903
Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P) ...
 13-156 4.62e-49

Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P)H-flavin oxidoreductases (flavin reductases), a class of enzymes capable of producing reduced flavin for bacterial bioluminescence and other biological processes. This domain is also found in various other oxidoreductase and monooxygenase enzymes... This domain consists of a beta-barrel with Greek key topology, and is related to the ferredoxin reductase-like FAD-binding domain. The flavin reductases have a different dimerisation mode than that found in the PNP oxidase-like family, which also carries an FMN-binding domain with a similar topology.


Pssm-ID: 214900  Cd Length: 147  Bit Score: 155.01  E-value: 4.62e-49
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202     13 MSCMGAAVNIITTDGPAG-RAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPM 91
Cdd:smart00903   1 LGRFPTGVAVVTTRDGDGgRVGLTASSFVSVSLDPPLVLVCVAKSSSTHPLIRESGRFVVNVLAEDQADLARRFAGKSGA 80

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 90111202     92 EhRFAAARWQT---GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSY 156
Cdd:smart00903  81 D-RFEGVAWGLteaGVTGAPILAGALAWLECRVVQVIEVGDHTIFVGEVVAVHVRDDGEPLVYHRGGY 147
Flavin_Reduct pfam01613
Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to ...
 13-159 2.48e-45

Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to be flavin reductases as well as various oxidoreductase and monooxygenase components. VlmR is a flavin reductase that functions in a two-component enzyme system to provide isobutylamine N-hydroxylase with reduced flavin and may be involved in the synthesis of valanimycin. SnaC is a flavin reductase that provides reduced flavin for the oxidation of pristinamycin IIB to pristinamycin IIA as catalyzed by SnaA, SnaB heterodimer. This flavin reductase region characterized by enzymes of the family is present in the C-terminus of potential FMN proteins from Synechocystis sp. suggesting it is a flavin reductase domain.


Pssm-ID: 460266  Cd Length: 149  Bit Score: 145.50  E-value: 2.48e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202    13 MSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPmE 92
Cdd:pfam01613   1 MRRFPTGVAVVTTDDGGEPNGMTVSSFTSVSLDPPLVLVSINRSSSTHDAIEASGEFVVNVLAEDQEELARRFAGRSG-R 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 90111202    93 HRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYG--LVWFDRSYHAL 159
Cdd:pfam01613  80 DKFAGITWVEGKVGAPLLKGALAALECRVVDTVDVGDHTLFIGEVVDVRVDEDADGepLLYYRRRYRSL 148
 
Name Accession Description Interval E-value
RutF TIGR03615
pyrimidine utilization flavin reductase protein F; This protein is observed in operons ...
 4-159 1.65e-106

pyrimidine utilization flavin reductase protein F; This protein is observed in operons extremely similar to that characterized in E. coli K-12 responsible for the import and catabolism of pyrimidines, primarily uracil. This protein is a member of the flavin reductase family defined by pfam01613. Presumably, this protein recycles the flavin of the RutA luciferase-like oxidoreductase.


Pssm-ID: 132654  Cd Length: 156  Bit Score: 300.95  E-value: 1.65e-106
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202     4 VDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSN 83
Cdd:TIGR03615   1 VDQQAFRDAMSRLGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRSASAYPAFKQNGTLCVNTLAAGQEDLSN 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 90111202    84 LFGGKTPMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHAL 159
Cdd:TIGR03615  81 LFGGKTPMEERFAAGQWRTLVTGAPVLEDALVSFDCRISQSHSVGTHDILFCAVEAIAHSAGADALVYFNRRYHTL 156
HpaC TIGR02296
4-hydroxyphenylacetate 3-monooxygenase, reductase component; This model identifies the ...
 9-160 1.20e-52

4-hydroxyphenylacetate 3-monooxygenase, reductase component; This model identifies the reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase. This enzyme catalyzes the first step (hydroxylation at the 3-position) in the degradation of 4-hydroxyphenylacetate to succinate and pyruvate. 4-hydroxyphenylacetate arises from the degradation of tyrosine. These reductases catalyze the reduction of free flavins by NADPH. The flavin is then utilized by the large subunit of the monooxygenase.


Pssm-ID: 131349  Cd Length: 154  Bit Score: 164.55  E-value: 1.20e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202     9 FRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGK 88
Cdd:TIGR02296   1 FRDAMASLAAAVNIITTNGIAGKCGITATAVCSVTDTPPTVMVCINRNSAMNPIFQENGKLCINVLAHEQQEMAEHFAGM 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 90111202    89 TP--MEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM 160
Cdd:TIGR02296  81 TGlaMEERFAWHIWHEGPDGQPVLKGALAHLEGHIVDKHEIGTHTVFLVELKEIKVNGKRHALVYFRRQFKFVD 154
hpaC PRK15486
4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional
 4-160 1.72e-50

4-hydroxyphenylacetate 3-monooxygenase reductase subunit; Provisional


Pssm-ID: 185383  Cd Length: 170  Bit Score: 159.44  E-value: 1.72e-50
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202    4 VDQQT--FRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPL 81
Cdd:PRK15486   3 LDEQRlrFRDAMASLSAAVNIVTTAGDAGRCGITATAVCSVTDTPPSVMVCINANSAMNPVFQGNGKLCINVLNHEQELM 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202   82 SNLFGGKT--PMEHRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHAL 159
Cdd:PRK15486  83 ARHFAGMTgmAMEERFSLSCWQKGPLGQPVLKGSLASLEGEISDVQTIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPV 162


                 .
gi 90111202  160 M 160
Cdd:PRK15486 163 M 163
RutF COG1853
FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, ...
 5-158 1.10e-49

FMN reductase RutF, DIM6/NTAB family [Energy production and conversion]; FMN reductase RutF, DIM6/NTAB family is part of the Pathway/BioSystem: Pyrimidine degradation


Pssm-ID: 441458  Cd Length: 160  Bit Score: 156.91  E-value: 1.10e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202   5 DQQTFRDAMSCMGAAVNIITTDGPAG-RAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSN 83
Cdd:COG1853   1 MPDAFRDALGRLAPGVAVVTTRDADGrPNGMTASSFTSVSLDPPLVLVSVAKSSDTLENIRETGEFVVNVLSEDQAELAN 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202  84 LFGGKT-PMEHRFAAARWQT--GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYG--LVWFDRSYHA 158
Cdd:COG1853  81 RFAGRSgRGVDKFAGAGLTTasGEVGAPLLAEAPAWLECRVVDVIELGDHTLFIGEVVAVHVDEDVDGrpLLYLGGRYRR 160
Flavin_Reduct smart00903
Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P) ...
 13-156 4.62e-49

Flavin reductase like domain; This entry represents the FMN-binding domain found in NAD(P)H-flavin oxidoreductases (flavin reductases), a class of enzymes capable of producing reduced flavin for bacterial bioluminescence and other biological processes. This domain is also found in various other oxidoreductase and monooxygenase enzymes... This domain consists of a beta-barrel with Greek key topology, and is related to the ferredoxin reductase-like FAD-binding domain. The flavin reductases have a different dimerisation mode than that found in the PNP oxidase-like family, which also carries an FMN-binding domain with a similar topology.


Pssm-ID: 214900  Cd Length: 147  Bit Score: 155.01  E-value: 4.62e-49
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202     13 MSCMGAAVNIITTDGPAG-RAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPM 91
Cdd:smart00903   1 LGRFPTGVAVVTTRDGDGgRVGLTASSFVSVSLDPPLVLVCVAKSSSTHPLIRESGRFVVNVLAEDQADLARRFAGKSGA 80

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 90111202     92 EhRFAAARWQT---GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSY 156
Cdd:smart00903  81 D-RFEGVAWGLteaGVTGAPILAGALAWLECRVVQVIEVGDHTIFVGEVVAVHVRDDGEPLVYHRGGY 147
Flavin_Reduct pfam01613
Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to ...
 13-159 2.48e-45

Flavin reductase like domain; This is a flavin reductase family consisting of enzymes known to be flavin reductases as well as various oxidoreductase and monooxygenase components. VlmR is a flavin reductase that functions in a two-component enzyme system to provide isobutylamine N-hydroxylase with reduced flavin and may be involved in the synthesis of valanimycin. SnaC is a flavin reductase that provides reduced flavin for the oxidation of pristinamycin IIB to pristinamycin IIA as catalyzed by SnaA, SnaB heterodimer. This flavin reductase region characterized by enzymes of the family is present in the C-terminus of potential FMN proteins from Synechocystis sp. suggesting it is a flavin reductase domain.


Pssm-ID: 460266  Cd Length: 149  Bit Score: 145.50  E-value: 2.48e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 90111202    13 MSCMGAAVNIITTDGPAGRAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTPmE 92
Cdd:pfam01613   1 MRRFPTGVAVVTTDDGGEPNGMTVSSFTSVSLDPPLVLVSINRSSSTHDAIEASGEFVVNVLAEDQEELARRFAGRSG-R 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 90111202    93 HRFAAARWQTGVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYG--LVWFDRSYHAL 159
Cdd:pfam01613  80 DKFAGITWVEGKVGAPLLKGALAALECRVVDTVDVGDHTLFIGEVVDVRVDEDADGepLLYYRRRYRSL 148
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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