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Conserved domains on  [gi|16130032|ref|NP_416597|]
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galactitol-specific PTS enzyme IIA component [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

PTS galactitol transporter subunit IIA( domain architecture ID 10793303)

PTS galactitol transporter subunit IIA is a component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane

EC:  2.7.1.200
Gene Ontology:  GO:0009401|GO:0015796|GO:0016310|GO:0090584
PubMed:  10393270

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK09665 PRK09665
PTS galactitol transporter subunit IIA;
1-150 2.22e-96

PTS galactitol transporter subunit IIA;


:

Pssm-ID: 182023 [Multi-domain]  Cd Length: 150  Bit Score: 274.37  E-value: 2.22e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032    1 MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTN 80
Cdd:PRK09665   1 MSNLFVRTGISFVDRSEALTHIGNEMLAKGVVHDTYPQALIAREAEFPTGIMLEQHAVAIPHCEAIHAKSPAIYLIRPDK 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE 150
Cdd:PRK09665  81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLLTLPETQLKEYFTKYVLDSDE 150
 
Name Accession Description Interval E-value
PRK09665 PRK09665
PTS galactitol transporter subunit IIA;
1-150 2.22e-96

PTS galactitol transporter subunit IIA;


Pssm-ID: 182023 [Multi-domain]  Cd Length: 150  Bit Score: 274.37  E-value: 2.22e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032    1 MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTN 80
Cdd:PRK09665   1 MSNLFVRTGISFVDRSEALTHIGNEMLAKGVVHDTYPQALIAREAEFPTGIMLEQHAVAIPHCEAIHAKSPAIYLIRPDK 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE 150
Cdd:PRK09665  81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLLTLPETQLKEYFTKYVLDSDE 150
PTS_IIA_fru cd00211
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 1-141 2.82e-28

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238129 [Multi-domain]  Cd Length: 136  Bit Score: 101.10  E-value: 2.82e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   1 MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMleqHAIAIPHCEAIHAKSSAIYLLRPTN 80
Cdd:cd00211   1 LTKENIRLNLKAKSKEEAIEELAQLLVAAGYVEEEYIEALLEREKEGSTGIG---NGIAIPHAKSEAVKKPGIAVLRLKE 77

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 16130032  81 KVHFQQADDDndvAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLP-ETQLKEYF 141
Cdd:cd00211  78 PVDFGSLDGQ---PVHLIFLLAAPDSNEHLKALSQLARLLSDEEFVEQLLNAQsKEEILALL 136
PtsN COG1762
Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate ...
 5-144 6.84e-22

Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate transport and metabolism, Signal transduction mechanisms];


Pssm-ID: 441368 [Multi-domain]  Cd Length: 150  Bit Score: 85.29  E-value: 6.84e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   5 FVRSGISFVDRSEVLTHIGNEMLAKGVVHDT--WPQALIAREAEFPTGImleQHAIAIPHCEAIHAKSSAIYLLRPTNKV 82
Cdd:COG1762  11 LILLDLEASSKEEAIEELAELLAEKGYVLDKeeYLEALLEREELGSTGI---GPGIAIPHARPEGVKKPGIAVARLKEPV 87

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 16130032  83 HFQQADDDNdvaVSLVIALIV--ENPQQQLKLLRCLFGKLQQPDIVETLITLP-ETQLKEYFTKY 144
Cdd:COG1762  88 DFGAMDGEP---VDLVFLLAApeDDSEEHLKLLAELARLLSDEEFREKLLNAKsPEEILELLKEA 149
PTS_EIIA_2 pfam00359
Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;
5-131 1.97e-17

Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;


Pssm-ID: 459780 [Multi-domain]  Cd Length: 139  Bit Score: 73.39  E-value: 1.97e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032     5 FVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEqhaIAIPHCEAIHAKSSAIYLLRPTNKVHF 84
Cdd:pfam00359   6 LIFLNLEAKSKEEAIEFLADKLVEAGYVEPAYLEAILEREKEGSTGIGNG---IAIPHARSEAVKKPGIAVLTLKEPVDF 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 16130032    85 QQADDDNdvaVSLVIALIV-ENPQQQLKLLRCLFGKLQQPDIVETLIT 131
Cdd:pfam00359  83 GSEDGKP---VKLIFLLAApDNEASHLKILSQLARLLQDEEFVEKLLK 127
fruA TIGR00848
PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family ...
 10-129 2.24e-07

PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several putative PTS permeases of unknown specificities. The fructose permeases of this family phosphorylate fructose on the 1-position. Those of family 4.6 phosphorylate fructose on the 6-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This model is specific for the IIA domain of the fructose PTS transporters. Also similar to the Enzyme IIA Fru subunits of the PTS, but included in TIGR01419 rather than this model, is enzyme IIA Ntr (nitrogen), also called PtsN, found in E. coli and other organisms, which may play a solely regulatory role. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273298 [Multi-domain]  Cd Length: 129  Bit Score: 46.88  E-value: 2.24e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032    10 ISFVDRSEVLTHIGNEMLAKGVVHDT--WPQALIAREAEFPTGIMleqHAIAIPHCEAIHAKSSAIYLLRPTNKVHFqQA 87
Cdd:TIGR00848  11 QQFTSKEDVIKFLANKLLENGYISDTeeFLEDLLKREEEGTTGIG---DGVAIPHAKSAAVKQPFVAIARLVKGVDW-QS 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 16130032    88 DDDNDVAVSLVIALIVENPQ-QQLKLLRCLFGKLQQPDIVETL 129
Cdd:TIGR00848  87 LDGKPVKLIFLIAVPKDEAGnTHLKALSQLARLLLNDEFRAKL 129
 
Name Accession Description Interval E-value
PRK09665 PRK09665
PTS galactitol transporter subunit IIA;
1-150 2.22e-96

PTS galactitol transporter subunit IIA;


Pssm-ID: 182023 [Multi-domain]  Cd Length: 150  Bit Score: 274.37  E-value: 2.22e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032    1 MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTN 80
Cdd:PRK09665   1 MSNLFVRTGISFVDRSEALTHIGNEMLAKGVVHDTYPQALIAREAEFPTGIMLEQHAVAIPHCEAIHAKSPAIYLIRPDK 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE 150
Cdd:PRK09665  81 KVHFQQADDDNDVAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLLTLPETQLKEYFTKYVLDSDE 150
PTS_IIA_fru cd00211
PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: ...
 1-141 2.82e-28

PTS_IIA, PTS system, fructose/mannitol specific IIA subunit. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. This family is one of four structurally and functionally distinct group IIA PTS system cytoplasmic enzymes, necessary for the uptake of carbohydrates across the cytoplasmic membrane and their phosphorylation.


Pssm-ID: 238129 [Multi-domain]  Cd Length: 136  Bit Score: 101.10  E-value: 2.82e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   1 MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMleqHAIAIPHCEAIHAKSSAIYLLRPTN 80
Cdd:cd00211   1 LTKENIRLNLKAKSKEEAIEELAQLLVAAGYVEEEYIEALLEREKEGSTGIG---NGIAIPHAKSEAVKKPGIAVLRLKE 77

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 16130032  81 KVHFQQADDDndvAVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLP-ETQLKEYF 141
Cdd:cd00211  78 PVDFGSLDGQ---PVHLIFLLAAPDSNEHLKALSQLARLLSDEEFVEQLLNAQsKEEILALL 136
PtsN COG1762
Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate ...
 5-144 6.84e-22

Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type) [Carbohydrate transport and metabolism, Signal transduction mechanisms];


Pssm-ID: 441368 [Multi-domain]  Cd Length: 150  Bit Score: 85.29  E-value: 6.84e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   5 FVRSGISFVDRSEVLTHIGNEMLAKGVVHDT--WPQALIAREAEFPTGImleQHAIAIPHCEAIHAKSSAIYLLRPTNKV 82
Cdd:COG1762  11 LILLDLEASSKEEAIEELAELLAEKGYVLDKeeYLEALLEREELGSTGI---GPGIAIPHARPEGVKKPGIAVARLKEPV 87

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 16130032  83 HFQQADDDNdvaVSLVIALIV--ENPQQQLKLLRCLFGKLQQPDIVETLITLP-ETQLKEYFTKY 144
Cdd:COG1762  88 DFGAMDGEP---VDLVFLLAApeDDSEEHLKLLAELARLLSDEEFREKLLNAKsPEEILELLKEA 149
PTS_EIIA_2 pfam00359
Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;
5-131 1.97e-17

Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2;


Pssm-ID: 459780 [Multi-domain]  Cd Length: 139  Bit Score: 73.39  E-value: 1.97e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032     5 FVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPTGIMLEqhaIAIPHCEAIHAKSSAIYLLRPTNKVHF 84
Cdd:pfam00359   6 LIFLNLEAKSKEEAIEFLADKLVEAGYVEPAYLEAILEREKEGSTGIGNG---IAIPHARSEAVKKPGIAVLTLKEPVDF 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*...
gi 16130032    85 QQADDDNdvaVSLVIALIV-ENPQQQLKLLRCLFGKLQQPDIVETLIT 131
Cdd:pfam00359  83 GSEDGKP---VKLIFLLAApDNEASHLKILSQLARLLQDEEFVEKLLK 127
fruA TIGR00848
PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family ...
 10-129 2.24e-07

PTS system, fructose subfamily, IIA component; 4.A.2 The PTS Fructose-Mannitol (Fru) Family Bacterial PTS transporters transport and concomitantly phosphorylate their sugar substrates, and typically consist of multiple subunits or protein domains. The Fru family is a large and complex family which includes several sequenced fructose and mannitol-specific permeases as well as several putative PTS permeases of unknown specificities. The fructose permeases of this family phosphorylate fructose on the 1-position. Those of family 4.6 phosphorylate fructose on the 6-position. The Fru family PTS systems typically have 3 domains, IIA, IIB and IIC, which may be found as 1 or more proteins. The fructose and mannitol transporters form separate phylogenetic clusters in this family. This model is specific for the IIA domain of the fructose PTS transporters. Also similar to the Enzyme IIA Fru subunits of the PTS, but included in TIGR01419 rather than this model, is enzyme IIA Ntr (nitrogen), also called PtsN, found in E. coli and other organisms, which may play a solely regulatory role. [Transport and binding proteins, Carbohydrates, organic alcohols, and acids, Signal transduction, PTS]


Pssm-ID: 273298 [Multi-domain]  Cd Length: 129  Bit Score: 46.88  E-value: 2.24e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032    10 ISFVDRSEVLTHIGNEMLAKGVVHDT--WPQALIAREAEFPTGIMleqHAIAIPHCEAIHAKSSAIYLLRPTNKVHFqQA 87
Cdd:TIGR00848  11 QQFTSKEDVIKFLANKLLENGYISDTeeFLEDLLKREEEGTTGIG---DGVAIPHAKSAAVKQPFVAIARLVKGVDW-QS 86

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|...
gi 16130032    88 DDDNDVAVSLVIALIVENPQ-QQLKLLRCLFGKLQQPDIVETL 129
Cdd:TIGR00848  87 LDGKPVKLIFLIAVPKDEAGnTHLKALSQLARLLLNDEFRAKL 129
MtlA2 COG4668
Mannitol/fructose-specific phosphotransferase system, IIA domain [Carbohydrate transport and ...
 11-131 2.21e-06

Mannitol/fructose-specific phosphotransferase system, IIA domain [Carbohydrate transport and metabolism];


Pssm-ID: 443705 [Multi-domain]  Cd Length: 143  Bit Score: 44.38  E-value: 2.21e-06
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032  11 SFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAREAEFPT--GimleqHAIAIPH--CEAIHA-KSSAIYLLRPTNKVHFq 85
Cdd:COG4668  14 SAANKEEAIRLAGQLLVEAGYVEPEYIDAMLEREAQVSTylG-----NGIAIPHgtNEAKDLvLKTGISVLQFPDGVDW- 87

                        90       100       110       120
                ....*....|....*....|....*....|....*....|....*.
gi 16130032  86 qadDDNDVaVSLVIALIVENpQQQLKLLRCLFGKLQQPDIVETLIT 131
Cdd:COG4668  88 ---GDGNT-VYLVIGIAAKS-DEHLEILRQLARVLSDEENVEKLAK 128
PRK09913 PRK09913
PTS fructose transporter subunit IIA;
17-129 2.58e-06

PTS fructose transporter subunit IIA;


Pssm-ID: 182141 [Multi-domain]  Cd Length: 148  Bit Score: 44.49  E-value: 2.58e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16130032   17 EVLTHIGNEMLAKGVVHD--TWPQALIAREAEFPTGImleQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNdva 94
Cdd:PRK09913  20 SILKQLATIALQNGFITDshQFLQTLLLREKMHSTGF---GSGVAVPHGKSACVKQPFVLFARKAQAIDWQASDGED--- 93

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 16130032   95 VSLVIALIVenPQQ----QLKLLRCLFGKLQQPDIVETL 129
Cdd:PRK09913  94 VNCWICLGV--PQSgeedQVKIIGTLCRKIIHQDFIHQL 130
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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