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Conserved domains on  [gi|16131096|ref|NP_417673|]
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phosphorelay protein NPr [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

PTS phosphocarrier protein NPr( domain architecture ID 10793495)

PTS phosphocarrier protein NPr is part of the nitrogen PTS, a paralogous pathway to the PEP:sugar phosphotransferase system (PTS), which is believed to function in regulation, not transport. As other members of the HPr family phosphocarrier proteins it can be phosphorylated at an active-site histidine by phosphoenolpyruvate (PEP).

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRK10897 PRK10897
PTS phosphocarrier protein NPr;
1-90 5.92e-55

PTS phosphocarrier protein NPr;


:

Pssm-ID: 182819 [Multi-domain]  Cd Length: 90  Bit Score: 165.00  E-value: 5.92e-55
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096   1 MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80
Cdd:PRK10897  1 MTVKQTVEIKNKLGMHARPAMKLFELVQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80

                        90
                ....*....|
gi 16131096  81 ALFNSGFDED 90
Cdd:PRK10897 81 ALFNSGFDED 90
 
Name Accession Description Interval E-value
PRK10897 PRK10897
PTS phosphocarrier protein NPr;
1-90 5.92e-55

PTS phosphocarrier protein NPr;


Pssm-ID: 182819 [Multi-domain]  Cd Length: 90  Bit Score: 165.00  E-value: 5.92e-55
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096   1 MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80
Cdd:PRK10897  1 MTVKQTVEIKNKLGMHARPAMKLFELVQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80

                        90
                ....*....|
gi 16131096  81 ALFNSGFDED 90
Cdd:PRK10897 81 ALFNSGFDED 90
PtsH COG1925
HPr or related phosphotransfer protein [Signal transduction mechanisms, Carbohydrate transport ...
 2-90 1.09e-31

HPr or related phosphotransfer protein [Signal transduction mechanisms, Carbohydrate transport and metabolism];


Pssm-ID: 441528 [Multi-domain]  Cd Length: 88  Bit Score: 105.96  E-value: 1.09e-31
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096  2 TVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIA 81
Cdd:COG1925  1 MLEREVTIVNKLGLHARPAAKLVQLASKFDSEITVEKG-GKEVNAKSIMGLMSLGAKKGDEVTITAEGPDAEEALDALAA 79


               ....*....
gi 16131096 82 LFNSGFDED 90
Cdd:COG1925 80 LIESGFGEE 88
PTS-HPr pfam00381
PTS HPr component phosphorylation site;
5-84 3.52e-25

PTS HPr component phosphorylation site;


Pssm-ID: 459792 [Multi-domain]  Cd Length: 79  Bit Score: 88.98  E-value: 3.52e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    5 QTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFN 84
Cdd:pfam00381  1 KTVTITNPLGLHARPAALLVQLASKFDSDITLEKG-GKKVNAKSIMGLMSLGAKQGDEITISAEGEDEEEALEALAALLE 79
PTS-HPr_like cd00367
Histidine-containing phosphocarrier protein (HPr)-like proteins. HPr is a central component of ...
 6-83 2.34e-23

Histidine-containing phosphocarrier protein (HPr)-like proteins. HPr is a central component of the bacterial phosphoenolpyruvate sugar phosphotransferase system (PTS). The PTS catalyses the phosphorylation of sugar substrates during their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate is transferred to HPr by enzyme I (EI). Phospho-HPr then transfers the phosphoryl group to one of several sugar-specific phosphoprotein intermediates. The conserved histidine in the N-terminus of HPr serves as an acceptor for the phosphoryl group of EI. In addition to the phosphotransferase proteins HPr and E1, this family also includes the closely related Carbon Catabolite Repressor (CCR) proteins which use the same phosphorylation mechanism and interact with transcriptional regulators to control expression of genes coding for utilization of less favored carbon sources.


Pssm-ID: 238217 [Multi-domain]  Cd Length: 77  Bit Score: 84.48  E-value: 2.34e-23
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 16131096  6 TVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALF 83
Cdd:cd00367  1 TVTITNPLGLHARPAALLVQLASKFKSDITLRKG-GRKANAKSILGLMSLGAKQGDEITLSAEGEDAEEALEALAELL 77
PTS_HPr_family TIGR01003
Phosphotransferase System HPr (HPr) Family; The HPr family are bacterial proteins (or domains ...
 4-84 2.12e-18

Phosphotransferase System HPr (HPr) Family; The HPr family are bacterial proteins (or domains of proteins) which function in phosphoryl transfer system (PTS) systems. They include energy-coupling components which catalyze sugar uptake via a group translocation mechanism. The functions of most of these proteins are not known, but they presumably function in PTS-related regulatory capacities. All seed members are stand-alone HPr proteins, although the model also recognizes HPr domains of PTS fusion proteins. This family includes the related NPr protein. [Signal transduction, PTS]


Pssm-ID: 273389 [Multi-domain]  Cd Length: 82  Bit Score: 72.30  E-value: 2.12e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    4 KQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALF 83
Cdd:TIGR01003  3 SKEVTIINKVGLHARPAAILVKLASGFDSEITLTKN-GKEVNAKSIMGIMMLGAGQGTEVTVSADGEDEAEALEALAKLF 81


                 .
gi 16131096   84 N 84
Cdd:TIGR01003 82 E 82
 
Name Accession Description Interval E-value
PRK10897 PRK10897
PTS phosphocarrier protein NPr;
1-90 5.92e-55

PTS phosphocarrier protein NPr;


Pssm-ID: 182819 [Multi-domain]  Cd Length: 90  Bit Score: 165.00  E-value: 5.92e-55
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096   1 MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80
Cdd:PRK10897  1 MTVKQTVEIKNKLGMHARPAMKLFELVQGFDAEVLLRNDEGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80

                        90
                ....*....|
gi 16131096  81 ALFNSGFDED 90
Cdd:PRK10897 81 ALFNSGFDED 90
PtsH COG1925
HPr or related phosphotransfer protein [Signal transduction mechanisms, Carbohydrate transport ...
 2-90 1.09e-31

HPr or related phosphotransfer protein [Signal transduction mechanisms, Carbohydrate transport and metabolism];


Pssm-ID: 441528 [Multi-domain]  Cd Length: 88  Bit Score: 105.96  E-value: 1.09e-31
                       10        20        30        40        50        60        70        80
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096  2 TVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIA 81
Cdd:COG1925  1 MLEREVTIVNKLGLHARPAAKLVQLASKFDSEITVEKG-GKEVNAKSIMGLMSLGAKKGDEVTITAEGPDAEEALDALAA 79


               ....*....
gi 16131096 82 LFNSGFDED 90
Cdd:COG1925 80 LIESGFGEE 88
PTS-HPr pfam00381
PTS HPr component phosphorylation site;
5-84 3.52e-25

PTS HPr component phosphorylation site;


Pssm-ID: 459792 [Multi-domain]  Cd Length: 79  Bit Score: 88.98  E-value: 3.52e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    5 QTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFN 84
Cdd:pfam00381  1 KTVTITNPLGLHARPAALLVQLASKFDSDITLEKG-GKKVNAKSIMGLMSLGAKQGDEITISAEGEDEEEALEALAALLE 79
PTS-HPr_like cd00367
Histidine-containing phosphocarrier protein (HPr)-like proteins. HPr is a central component of ...
 6-83 2.34e-23

Histidine-containing phosphocarrier protein (HPr)-like proteins. HPr is a central component of the bacterial phosphoenolpyruvate sugar phosphotransferase system (PTS). The PTS catalyses the phosphorylation of sugar substrates during their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate is transferred to HPr by enzyme I (EI). Phospho-HPr then transfers the phosphoryl group to one of several sugar-specific phosphoprotein intermediates. The conserved histidine in the N-terminus of HPr serves as an acceptor for the phosphoryl group of EI. In addition to the phosphotransferase proteins HPr and E1, this family also includes the closely related Carbon Catabolite Repressor (CCR) proteins which use the same phosphorylation mechanism and interact with transcriptional regulators to control expression of genes coding for utilization of less favored carbon sources.


Pssm-ID: 238217 [Multi-domain]  Cd Length: 77  Bit Score: 84.48  E-value: 2.34e-23
                       10        20        30        40        50        60        70
               ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 16131096  6 TVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALF 83
Cdd:cd00367  1 TVTITNPLGLHARPAALLVQLASKFKSDITLRKG-GRKANAKSILGLMSLGAKQGDEITLSAEGEDAEEALEALAELL 77
PTS_HPr_family TIGR01003
Phosphotransferase System HPr (HPr) Family; The HPr family are bacterial proteins (or domains ...
 4-84 2.12e-18

Phosphotransferase System HPr (HPr) Family; The HPr family are bacterial proteins (or domains of proteins) which function in phosphoryl transfer system (PTS) systems. They include energy-coupling components which catalyze sugar uptake via a group translocation mechanism. The functions of most of these proteins are not known, but they presumably function in PTS-related regulatory capacities. All seed members are stand-alone HPr proteins, although the model also recognizes HPr domains of PTS fusion proteins. This family includes the related NPr protein. [Signal transduction, PTS]


Pssm-ID: 273389 [Multi-domain]  Cd Length: 82  Bit Score: 72.30  E-value: 2.12e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    4 KQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALF 83
Cdd:TIGR01003  3 SKEVTIINKVGLHARPAAILVKLASGFDSEITLTKN-GKEVNAKSIMGIMMLGAGQGTEVTVSADGEDEAEALEALAKLF 81


                 .
gi 16131096   84 N 84
Cdd:TIGR01003 82 E 82
PRK11377 PRK11377
dihydroxyacetone kinase subunit M; Provisional
 6-89 9.44e-12

dihydroxyacetone kinase subunit M; Provisional


Pssm-ID: 183108 [Multi-domain]  Cd Length: 473  Bit Score: 58.99  E-value: 9.44e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    6 TVEITNKLGMHARPAMKLFELMQGFDAEVLL-RNDEGTEAEANSVIALLMLdsAKGRQIEVEATGPQEEEALAAVIALFN 84
Cdd:PRK11377 159 SVVIKNRNGLHVRPASRLVYTLSTFNADMLLeKNGKCVTPESLNQIALLQV--RYNDTLRLIAKGPEAEEALIAFRQLAE 236


                 ....*
gi 16131096   85 SGFDE 89
Cdd:PRK11377 237 DNFGE 241
PRK13782 PRK13782
HPr family phosphocarrier protein;
3-81 1.28e-08

HPr family phosphocarrier protein;


Pssm-ID: 172320 [Multi-domain]  Cd Length: 82  Bit Score: 47.47  E-value: 1.28e-08
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 16131096   3 VKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIA 81
Cdd:PRK13782  2 VQKRVEVSLKTGLQARPAALFVQEANRFHADIFIEKD-GKKVNAKSIMGLMSLAIGTGSMITIITEGSDEEEALEALAA 79
PRK13780 PRK13780
phosphocarrier protein HPr; Provisional
 4-79 3.84e-07

phosphocarrier protein HPr; Provisional


Pssm-ID: 237503 [Multi-domain]  Cd Length: 88  Bit Score: 43.53  E-value: 3.84e-07
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 16131096   4 KQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAV 79
Cdd:PRK13780  3 SKDFHITAETGIHARPATLLVQTASKFDSDITLEYN-GKSVNLKSIMGVMSLGVGQGADITISAEGADAADAIAAI 77
PRK10850 PRK10850
phosphocarrier protein Hpr;
4-82 8.03e-07

phosphocarrier protein Hpr;


Pssm-ID: 182777 [Multi-domain]  Cd Length: 85  Bit Score: 42.81  E-value: 8.03e-07
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 16131096   4 KQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDeGTEAEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIAL 82
Cdd:PRK10850  3 QQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSN-GKSASAKSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKL 80
PRK13779 PRK13779
bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein; Provisional
 3-89 1.63e-06

bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein; Provisional


Pssm-ID: 237502 [Multi-domain]  Cd Length: 503  Bit Score: 44.10  E-value: 1.63e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    3 VKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRN-DEGTE-AEANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVI 80
Cdd:PRK13779 415 VEGVFVIKNEHGLHARPSAVLVNEVKKYNASVAVQNlDRNTQlVSAKSLMKIVALGVVKGHRLRFVATGEEAQQAIDGIG 494


                 ....*....
gi 16131096   81 ALFNSGFDE 89
Cdd:PRK13779 495 AAIAAGLGE 503
PRK13779 PRK13779
bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein; Provisional
 2-89 8.51e-06

bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein; Provisional


Pssm-ID: 237502 [Multi-domain]  Cd Length: 503  Bit Score: 42.17  E-value: 8.51e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    2 TVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRN-DEGTEA-EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAV 79
Cdd:PRK13779 290 AVIGTFTIRNEHGLHARPSANLVNEVKKFTAKITVENlTRGSAPvSAKSLMKIVALGVTQGHRLRFVAEGEDAQQAIEAL 369

                         90
                 ....*....|
gi 16131096   80 IALFNSGFDE 89
Cdd:PRK13779 370 AKAIAEGLGE 379
PRK11109 PRK11109
fused PTS fructose transporter subunit IIA/HPr protein;
9-89 9.68e-06

fused PTS fructose transporter subunit IIA/HPr protein;


Pssm-ID: 236849 [Multi-domain]  Cd Length: 375  Bit Score: 41.86  E-value: 9.68e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131096    9 ITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEAEAN--SVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSG 86
Cdd:PRK11109 292 IRNEHGLHARPGAMLVNTIKQFNSEITVTNLDGTGKPANgrSLMKVVALGVKKGHRLRFTAQGEDAEQALKAIGEAIAAG 371


                 ...
gi 16131096   87 FDE 89
Cdd:PRK11109 372 LGE 374
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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