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Conserved domains on  [gi|16131210|ref|NP_417790|]
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Type II secretion system protein GspJ [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

type II secretion system protein J( domain architecture ID 11469172)

type II secretion system protein J is a component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 3-115 8.14e-18

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


:

Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 75.44  E-value: 8.14e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210   3 NRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKEIQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVL-GE 81
Cdd:COG4795   6 RRQRGFTLLELLVALAIFALLLLAAYRGLDSVLRSRERLEQQAERLQELQRALALLERDLRQAGPRPDEGGDPEPALsGG 85

                        90       100       110
                ....*....|....*....|....*....|....
gi 16131210  82 EAIFTTQSRDPLAPLSEAQtLLTVHWYLRNHTLY 115
Cdd:COG4795  86 GSCLIDLNRNGWWNPLRSE-LQRVGYRLRDGALE 118
 
Name Accession Description Interval E-value
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 3-115 8.14e-18

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 75.44  E-value: 8.14e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210   3 NRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKEIQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVL-GE 81
Cdd:COG4795   6 RRQRGFTLLELLVALAIFALLLLAAYRGLDSVLRSRERLEQQAERLQELQRALALLERDLRQAGPRPDEGGDPEPALsGG 85

                        90       100       110
                ....*....|....*....|....*....|....
gi 16131210  82 EAIFTTQSRDPLAPLSEAQtLLTVHWYLRNHTLY 115
Cdd:COG4795  86 GSCLIDLNRNGWWNPLRSE-LQRVGYRLRDGALE 118
gspJ TIGR01711
type II secretion system protein J; This model represents GspJ, one of two proteins highly ...
 7-174 3.05e-15

type II secretion system protein J; This model represents GspJ, one of two proteins highly conserved at their N-termini and described by pfam02501 but easily separable phylogenetically. The other is GspI. Both GspI and GspJ are proteins of the type II secretion pathway, or main terminal branch of the general secretion pathway. This pathway carries proteins across the outer membrane. Note that proteins of type II secretion are cryptic in E. coli K-12 - present but not yet demonstrated to act on any target.


Pssm-ID: 130772 [Multi-domain]  Cd Length: 192  Bit Score: 70.33  E-value: 3.05e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210     7 GFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKEIQQFNQLQRTITILDNDLLQLVARRNRST-DKIMVLGEEAIF 85
Cdd:TIGR01711   2 GFTLLELLVAIAIFASLSLGAYQVLDSVMQSDEATRVQEARLRELQRAMGAMERDLTQMVERPVRDDgEASEQDLRGAFL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    86 TTQS------------RDPLAPLSEAQtLLTVHWYLRNHTLYRAVRTSVDGRKDQPA--QAMLEHVES----FLLESN-S 146
Cdd:TIGR01711  82 SEGSddqgveftrggwRNPLGQQPRSR-LQRVGWRLSGETLERRYWLYPDRAQGSKPriQPVLDGVTAlswrFYDKGNwQ 160

                         170       180       190
                  ....*....|....*....|....*....|.
gi 16131210   147 GE---SQELPLSVTLHLQTQQYGGLQRRFAL 174
Cdd:TIGR01711 161 GEwptDNSLPLAVEVTLELRDYGKLVRVWLL 191
T2SSJ pfam11612
Type II secretion system (T2SS), protein J; The T2SJ proteins are pseudopilins, which are ...
 58-172 6.19e-07

Type II secretion system (T2SS), protein J; The T2SJ proteins are pseudopilins, which are targeted to the membrane in E. Coli. T2SJ forms a complex with T2SI (pfam02501) and T2SK (pfam03934) which is part of the Type II secretion apparatus involved in the translocation of proteins across the outer membrane in E.coli. The T2SK-I-J complex has quasihelical characteriztics.


Pssm-ID: 431959  Cd Length: 137  Bit Score: 46.93  E-value: 6.19e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    58 LDNDLLQLVARRNRS---------TDKIMVLGEEAI-FTTQS-RDPL-APLSEaqtLLTVHWYLRNHTLYRAVRTSVDGR 125
Cdd:pfam11612   1 LERDLSQAVPRPVRDaggdpapafAGRGLDSDDPGLeFTRLGwPNPDgAPRSE---LQRVGYRLEDGRLERLTWPYLDGA 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210   126 KDQPAQA--MLEHVESFLLE-----------SNSGESQELPLSVTLHLQTQQYGGLQRRF 172
Cdd:pfam11612  78 VGQEPQVqvLLDGVESLRLRflddgqwqdrwPPAAADQALPRAVEITLELKDGGEIRRLF 137
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 5-76 5.03e-05

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 41.45  E-value: 5.03e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    5 QQGFTLLEVMAALAIFS-MLSVLAFMIFSQASELH---QRSQKEIQQF-NQLQRTI-----TILDNDLLQLVARRNRSTD 74
Cdd:NF041002   1 EKGFTLLECLVALLVLSlILLLIPLLIKLLLKVEQylnNSDQLEWLLFlQQLESELkestfKVKNNKLYVKLDLIKSNGK 80


                 ..
gi 16131210   75 KI 76
Cdd:NF041002  81 TV 82
PRK10574 PRK10574
putative major pilin subunit; Provisional
 3-24 7.79e-04

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 38.09  E-value: 7.79e-04
                         10        20
                 ....*....|....*....|..
gi 16131210    3 NRQQGFTLLEVMAALAIFSMLS 24
Cdd:PRK10574   2 DKQRGFTLIELMVVIAIIAILS 23
 
Name Accession Description Interval E-value
PulJ COG4795
Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and ...
 3-115 8.14e-18

Type II secretory pathway, PulJ/GspJ component [Intracellular trafficking, secretion, and vesicular transport];


Pssm-ID: 443823 [Multi-domain]  Cd Length: 118  Bit Score: 75.44  E-value: 8.14e-18
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210   3 NRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKEIQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVL-GE 81
Cdd:COG4795   6 RRQRGFTLLELLVALAIFALLLLAAYRGLDSVLRSRERLEQQAERLQELQRALALLERDLRQAGPRPDEGGDPEPALsGG 85

                        90       100       110
                ....*....|....*....|....*....|....
gi 16131210  82 EAIFTTQSRDPLAPLSEAQtLLTVHWYLRNHTLY 115
Cdd:COG4795  86 GSCLIDLNRNGWWNPLRSE-LQRVGYRLRDGALE 118
gspJ TIGR01711
type II secretion system protein J; This model represents GspJ, one of two proteins highly ...
 7-174 3.05e-15

type II secretion system protein J; This model represents GspJ, one of two proteins highly conserved at their N-termini and described by pfam02501 but easily separable phylogenetically. The other is GspI. Both GspI and GspJ are proteins of the type II secretion pathway, or main terminal branch of the general secretion pathway. This pathway carries proteins across the outer membrane. Note that proteins of type II secretion are cryptic in E. coli K-12 - present but not yet demonstrated to act on any target.


Pssm-ID: 130772 [Multi-domain]  Cd Length: 192  Bit Score: 70.33  E-value: 3.05e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210     7 GFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKEIQQFNQLQRTITILDNDLLQLVARRNRST-DKIMVLGEEAIF 85
Cdd:TIGR01711   2 GFTLLELLVAIAIFASLSLGAYQVLDSVMQSDEATRVQEARLRELQRAMGAMERDLTQMVERPVRDDgEASEQDLRGAFL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    86 TTQS------------RDPLAPLSEAQtLLTVHWYLRNHTLYRAVRTSVDGRKDQPA--QAMLEHVES----FLLESN-S 146
Cdd:TIGR01711  82 SEGSddqgveftrggwRNPLGQQPRSR-LQRVGWRLSGETLERRYWLYPDRAQGSKPriQPVLDGVTAlswrFYDKGNwQ 160

                         170       180       190
                  ....*....|....*....|....*....|.
gi 16131210   147 GE---SQELPLSVTLHLQTQQYGGLQRRFAL 174
Cdd:TIGR01711 161 GEwptDNSLPLAVEVTLELRDYGKLVRVWLL 191
PulG COG2165
Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, ...
 3-61 2.12e-08

Type II secretory pathway, pseudopilin PulG [Cell motility, Intracellular trafficking, secretion, and vesicular transport, Extracellular structures];


Pssm-ID: 441768 [Multi-domain]  Cd Length: 99  Bit Score: 49.91  E-value: 2.12e-08
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 16131210   3 NRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASElHQRSQKEIQQFNQLQRTITILDND 61
Cdd:COG2165   8 RRQRGFTLIELLVVIAIIGILAALALPALQGARE-RARRAELRSNLRQIQQALERYRLD 65
T2SSJ pfam11612
Type II secretion system (T2SS), protein J; The T2SJ proteins are pseudopilins, which are ...
 58-172 6.19e-07

Type II secretion system (T2SS), protein J; The T2SJ proteins are pseudopilins, which are targeted to the membrane in E. Coli. T2SJ forms a complex with T2SI (pfam02501) and T2SK (pfam03934) which is part of the Type II secretion apparatus involved in the translocation of proteins across the outer membrane in E.coli. The T2SK-I-J complex has quasihelical characteriztics.


Pssm-ID: 431959  Cd Length: 137  Bit Score: 46.93  E-value: 6.19e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    58 LDNDLLQLVARRNRS---------TDKIMVLGEEAI-FTTQS-RDPL-APLSEaqtLLTVHWYLRNHTLYRAVRTSVDGR 125
Cdd:pfam11612   1 LERDLSQAVPRPVRDaggdpapafAGRGLDSDDPGLeFTRLGwPNPDgAPRSE---LQRVGYRLEDGRLERLTWPYLDGA 77

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210   126 KDQPAQA--MLEHVESFLLE-----------SNSGESQELPLSVTLHLQTQQYGGLQRRF 172
Cdd:pfam11612  78 VGQEPQVqvLLDGVESLRLRflddgqwqdrwPPAAADQALPRAVEITLELKDGGEIRRLF 137
N_methyl pfam07963
Prokaryotic N-terminal methylation motif; This short motif directs methylation of the ...
 3-28 1.46e-06

Prokaryotic N-terminal methylation motif; This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see pfam00114, pfam05946, pfam02501 and pfam07596.


Pssm-ID: 429756 [Multi-domain]  Cd Length: 27  Bit Score: 43.13  E-value: 1.46e-06
                          10        20
                  ....*....|....*....|....*.
gi 16131210     3 NRQQGFTLLEVMAALAIFSMLSVLAF 28
Cdd:pfam07963   2 RKQRGFTLIELLVALAILAILLAAAL 27
PilW COG4966
Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];
3-64 2.29e-06

Type IV pilus assembly protein PilW [Cell motility, Extracellular structures];


Pssm-ID: 443992 [Multi-domain]  Cd Length: 158  Bit Score: 45.56  E-value: 2.29e-06
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 16131210   3 NRQQGFTLLEVMAALAIFS--MLSVLAFMIFSQASELHQRSQKEIQQfnQLQRTITILDNDLLQ 64
Cdd:COG4966   2 RRQRGFTLVELMVALAIGLivLAAVLQLFLSSRRSYRTQEALARLQE--NGRFALDLLSRDLRQ 63
PilV COG4967
Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];
1-42 2.60e-05

Type IV pilus assembly protein PilV [Cell motility, Extracellular structures];


Pssm-ID: 443993 [Multi-domain]  Cd Length: 86  Bit Score: 41.12  E-value: 2.60e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*.
gi 16131210   1 MINRQQGFTLLEVMAALAIFS--MLSVLAFMIFS--QASELHQRSQ 42
Cdd:COG4967   6 RRRRQRGFTLIEVLVALVILSigLLGLAGLQAASlrSSQDARQRTQ 51
IV_pilin_GFxxxE TIGR02532
prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all ...
 5-27 3.01e-05

prepilin-type N-terminal cleavage/methylation domain; This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14). [Cell envelope, Surface structures, Protein fate, Protein and peptide secretion and trafficking]


Pssm-ID: 274182 [Multi-domain]  Cd Length: 24  Bit Score: 39.60  E-value: 3.01e-05
                          10        20
                  ....*....|....*....|...
gi 16131210     5 QQGFTLLEVMAALAIFSMLSVLA 27
Cdd:TIGR02532   1 QRGFTLIELLVVLAILGILALIA 23
pilin_ComGF NF041002
competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type ...
 5-76 5.03e-05

competence type IV pilus minor pilin ComGF; ComGF is one of several minor pilins in the type IV pilus involved in the competence system of natural transformation by various Gram-positive bacteria. Oddly, while nearly all members have an N-terminal region with a consensus site for cleavage and methylation, ComGF from Bacillus subtilis appears N-terminally truncated, and may be functional despite the truncation.


Pssm-ID: 468931 [Multi-domain]  Cd Length: 138  Bit Score: 41.45  E-value: 5.03e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    5 QQGFTLLEVMAALAIFS-MLSVLAFMIFSQASELH---QRSQKEIQQF-NQLQRTI-----TILDNDLLQLVARRNRSTD 74
Cdd:NF041002   1 EKGFTLLECLVALLVLSlILLLIPLLIKLLLKVEQylnNSDQLEWLLFlQQLESELkestfKVKNNKLYVKLDLIKSNGK 80


                 ..
gi 16131210   75 KI 76
Cdd:NF041002  81 TV 82
PilA COG4969
Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];
1-36 8.17e-05

Type IV pilus assembly protein, major pilin PilA [Cell motility, Extracellular structures];


Pssm-ID: 443995 [Multi-domain]  Cd Length: 134  Bit Score: 40.85  E-value: 8.17e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 16131210   1 MINRQQGFTLLEVMAALAIFSMLSVLA------FMIFSQASE 36
Cdd:COG4969   1 MKKKQKGFTLIELMIVVAIIGILAAIAipayqdYVARARVSE 42
FimT COG4970
Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];
1-55 1.64e-04

Type IV pilus assembly protein FimT [Cell motility, Extracellular structures];


Pssm-ID: 443996 [Multi-domain]  Cd Length: 73  Bit Score: 38.67  E-value: 1.64e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*
gi 16131210   1 MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQAselhQRSQKEIQQFNQLQRTI 55
Cdd:COG4970   4 LRRRQRGFTLIELLVVLAILAILAAIAVPSFSSL----IARQRLRAAANELAAAL 54
PilE COG4968
Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];
1-28 2.65e-04

Type IV pilus assembly protein PilE [Cell motility, Extracellular structures];


Pssm-ID: 443994 [Multi-domain]  Cd Length: 124  Bit Score: 39.29  E-value: 2.65e-04
                        10        20
                ....*....|....*....|....*...
gi 16131210   1 MINRQQGFTLLEVMAALAIFSMLSVLAF 28
Cdd:COG4968   5 MRRRQRGFTLIELMIVVAIIGILAAIAI 32
PRK10574 PRK10574
putative major pilin subunit; Provisional
 3-24 7.79e-04

putative major pilin subunit; Provisional


Pssm-ID: 236718 [Multi-domain]  Cd Length: 146  Bit Score: 38.09  E-value: 7.79e-04
                         10        20
                 ....*....|....*....|..
gi 16131210    3 NRQQGFTLLEVMAALAIFSMLS 24
Cdd:PRK10574   2 DKQRGFTLIELMVVIAIIAILS 23
ComGC COG4537
Competence protein ComGC [Mobilome: prophages, transposons];
1-27 1.29e-03

Competence protein ComGC [Mobilome: prophages, transposons];


Pssm-ID: 443603 [Multi-domain]  Cd Length: 108  Bit Score: 36.83  E-value: 1.29e-03
                        10        20
                ....*....|....*....|....*..
gi 16131210   1 MINRQQGFTLLEVMAALAIFSMLSVLA 27
Cdd:COG4537   7 KLKKEKGFTLIEMLIVLLIISILLLIA 33
PRK10506 PRK10506
prepilin peptidase-dependent protein;
2-25 1.70e-03

prepilin peptidase-dependent protein;


Pssm-ID: 236704 [Multi-domain]  Cd Length: 162  Bit Score: 37.28  E-value: 1.70e-03
                         10        20
                 ....*....|....*....|....
gi 16131210    2 INRQQGFTLLEVMAALAIFSMLSV 25
Cdd:PRK10506   5 MKKQRGYTLIELLVVMTIVSILSA 28
PRK08808 PRK08808
type II secretion system protein J;
1-105 2.20e-03

type II secretion system protein J;


Pssm-ID: 181557 [Multi-domain]  Cd Length: 211  Bit Score: 37.50  E-value: 2.20e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131210    1 MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQkEIQQFNQLQRTItildndllQLVARRNRSTDKIMVLG 80
Cdd:PRK08808   2 MRRRAAGFTLIEVLLATVLLVGGLALAFATLRSASAVSQRGE-AIAQRSERMRAV--------EEFLRRRLAGALPIAMG 72

                         90       100
                 ....*....|....*....|....*
gi 16131210   81 EEaiftTQSRDPLAPLSEAQTLLTV 105
Cdd:PRK08808  73 ID----TQSQQPILFVGEPQRMRFA 93
ComGF COG4940
Competence protein ComGF [Mobilome: prophages, transposons];
7-52 6.40e-03

Competence protein ComGF [Mobilome: prophages, transposons];


Pssm-ID: 443967 [Multi-domain]  Cd Length: 153  Bit Score: 35.76  E-value: 6.40e-03
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 16131210   7 GFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRS----QKEIQQF-NQLQ 52
Cdd:COG4940  17 GFTLLEKLAALMIIAYILAVLELLLKLLLKLNQSLdageQIEWELFiEQLI 67
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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