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Conserved domains on  [gi|16131592|ref|NP_418180|]
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negative regulator of the pho regulon [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

phosphate uptake regulator PhoU( domain architecture ID 11485211)

phosphate uptake regulator PhoU is a phosphate transport system regulatory protein similar to Escherichia coli PhoU, which acts as a negative regulator of the Pho regulon

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PRK11115 PRK11115
phosphate signaling complex protein PhoU;
1-236 4.04e-175

phosphate signaling complex protein PhoU;


:

Pssm-ID: 182974 [Multi-domain]  Cd Length: 236  Bit Score: 480.69  E-value: 4.04e-175
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    1 MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQ 80
Cdd:PRK11115   1 MDNLNLNKHISGQFNAELESIRTQVLTMGGLVEQQLSDAITAMHNQDAELAKRVIEGDHKVNMMEVAIDEACVRIIAKRQ 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592   81 PTASDLRLVMVISKTIAELERIGDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE 160
Cdd:PRK11115  81 PTASDLRLVMAIIKTIADLERIGDVADKIARTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDLDEAVRIYRE 160
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 16131592  161 DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRHVGGDELDKLLAG 236
Cdd:PRK11115 161 DKKVDQEYEGIVRQLMTYMMEDPRTIPSVLTVLWCARSIERIGDRCQNICEYIIYFVKGKDVRHVGGDELDKLLAG 236
 
Name Accession Description Interval E-value
PRK11115 PRK11115
phosphate signaling complex protein PhoU;
1-236 4.04e-175

phosphate signaling complex protein PhoU;


Pssm-ID: 182974 [Multi-domain]  Cd Length: 236  Bit Score: 480.69  E-value: 4.04e-175
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    1 MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQ 80
Cdd:PRK11115   1 MDNLNLNKHISGQFNAELESIRTQVLTMGGLVEQQLSDAITAMHNQDAELAKRVIEGDHKVNMMEVAIDEACVRIIAKRQ 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592   81 PTASDLRLVMVISKTIAELERIGDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE 160
Cdd:PRK11115  81 PTASDLRLVMAIIKTIADLERIGDVADKIARTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDLDEAVRIYRE 160
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 16131592  161 DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRHVGGDELDKLLAG 236
Cdd:PRK11115 161 DKKVDQEYEGIVRQLMTYMMEDPRTIPSVLTVLWCARSIERIGDRCQNICEYIIYFVKGKDVRHVGGDELDKLLAG 236
PhoU COG0704
Phosphate uptake regulator PhoU [Inorganic ion transport and metabolism];
16-224 2.07e-88

Phosphate uptake regulator PhoU [Inorganic ion transport and metabolism];


Pssm-ID: 440468 [Multi-domain]  Cd Length: 213  Bit Score: 260.52  E-value: 2.07e-88
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  16 AELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKT 95
Cdd:COG0704   1 EELEELRNDLLEMGGLVEEALEDAIEALLERDLELAEEVIEGDDEIDALEVEIEEECLRLIALQQPVASDLRLIVAALKI 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  96 IAELERIGDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVRQL 175
Cdd:COG0704  81 ATDLERIGDLAVNIAKRALRLAPEPPLKPLVDIEEMAELALEMLKDALDAFARRDVELAREVAERDDEVDELYRSIFREL 160
                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*....
gi 16131592 176 MTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRH 224
Cdd:COG0704 161 LTYMMEDPRNIEQAIDLLLIARYLERIGDHATNIAERVIYLVTGEDVRH 209
phoU_full TIGR02135
phosphate transport system regulatory protein PhoU; This model describes PhoU, a regulatory ...
13-222 6.00e-73

phosphate transport system regulatory protein PhoU; This model describes PhoU, a regulatory protein of unknown mechanism for high-affinity phosphate ABC transporter systems. The protein consists of two copies of the domain described by pfam01895. Deletion of PhoU activates constitutive expression of the phosphate ABC transporter and allows phosphate transport, but causes a growth defect and so likely has some second function. [Regulatory functions, Other, Transport and binding proteins, Anions]


Pssm-ID: 273990 [Multi-domain]  Cd Length: 212  Bit Score: 220.98  E-value: 6.00e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    13 QFNAELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVI 92
Cdd:TIGR02135   2 RFDEELKELREELLEMGGLVEEQLEDAVRALTEKDRELARKVIEDDDQINALEVKIEEKCLRLIALQQPVAKDLRLIISI 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    93 SKTIAELERIGDVADKICRTALE-KFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGI 171
Cdd:TIGR02135  82 IKISSDLERIGDYAVNIAKRALRlKEEDAKPKHLEELEKMGKLALKMLKDALDAFLNKDAELARQVAEMDERVDELYRQI 161
                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 16131592   172 VRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDF 222
Cdd:TIGR02135 162 FRELVTYMIEDPTNIEAALDVLLIARYLERIGDHATNIAERVIYIKTGKIV 212
PhoU pfam01895
PhoU domain; This family contains phosphate regulatory proteins including PhoU. PhoU proteins ...
28-111 5.60e-19

PhoU domain; This family contains phosphate regulatory proteins including PhoU. PhoU proteins are known to play a role in the regulation of phosphate uptake. The PhoU domain is composed of a three helix bundle. The PhoU protein contains two copies of this domain. The domain binds to an iron cluster via its conserved E/DXXXD motif.


Pssm-ID: 426500 [Multi-domain]  Cd Length: 87  Bit Score: 78.54  E-value: 5.60e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    28 MGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERIGDVAD 107
Cdd:pfam01895   2 MGELVEEMLDDALEALENRDVELAREVIELDDEIDRLYLEIERELLLLLALQQPLASDLRLIIALLKIARDLERIGDHAV 81

                  ....
gi 16131592   108 KICR 111
Cdd:pfam01895  82 NIAE 85
 
Name Accession Description Interval E-value
PRK11115 PRK11115
phosphate signaling complex protein PhoU;
1-236 4.04e-175

phosphate signaling complex protein PhoU;


Pssm-ID: 182974 [Multi-domain]  Cd Length: 236  Bit Score: 480.69  E-value: 4.04e-175
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    1 MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQ 80
Cdd:PRK11115   1 MDNLNLNKHISGQFNAELESIRTQVLTMGGLVEQQLSDAITAMHNQDAELAKRVIEGDHKVNMMEVAIDEACVRIIAKRQ 80
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592   81 PTASDLRLVMVISKTIAELERIGDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE 160
Cdd:PRK11115  81 PTASDLRLVMAIIKTIADLERIGDVADKIARTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDLDEAVRIYRE 160
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 16131592  161 DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRHVGGDELDKLLAG 236
Cdd:PRK11115 161 DKKVDQEYEGIVRQLMTYMMEDPRTIPSVLTVLWCARSIERIGDRCQNICEYIIYFVKGKDVRHVGGDELDKLLAG 236
PhoU COG0704
Phosphate uptake regulator PhoU [Inorganic ion transport and metabolism];
16-224 2.07e-88

Phosphate uptake regulator PhoU [Inorganic ion transport and metabolism];


Pssm-ID: 440468 [Multi-domain]  Cd Length: 213  Bit Score: 260.52  E-value: 2.07e-88
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  16 AELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKT 95
Cdd:COG0704   1 EELEELRNDLLEMGGLVEEALEDAIEALLERDLELAEEVIEGDDEIDALEVEIEEECLRLIALQQPVASDLRLIVAALKI 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  96 IAELERIGDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVRQL 175
Cdd:COG0704  81 ATDLERIGDLAVNIAKRALRLAPEPPLKPLVDIEEMAELALEMLKDALDAFARRDVELAREVAERDDEVDELYRSIFREL 160
                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*....
gi 16131592 176 MTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDFRH 224
Cdd:COG0704 161 LTYMMEDPRNIEQAIDLLLIARYLERIGDHATNIAERVIYLVTGEDVRH 209
phoU_full TIGR02135
phosphate transport system regulatory protein PhoU; This model describes PhoU, a regulatory ...
13-222 6.00e-73

phosphate transport system regulatory protein PhoU; This model describes PhoU, a regulatory protein of unknown mechanism for high-affinity phosphate ABC transporter systems. The protein consists of two copies of the domain described by pfam01895. Deletion of PhoU activates constitutive expression of the phosphate ABC transporter and allows phosphate transport, but causes a growth defect and so likely has some second function. [Regulatory functions, Other, Transport and binding proteins, Anions]


Pssm-ID: 273990 [Multi-domain]  Cd Length: 212  Bit Score: 220.98  E-value: 6.00e-73
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    13 QFNAELESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVI 92
Cdd:TIGR02135   2 RFDEELKELREELLEMGGLVEEQLEDAVRALTEKDRELARKVIEDDDQINALEVKIEEKCLRLIALQQPVAKDLRLIISI 81
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    93 SKTIAELERIGDVADKICRTALE-KFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGI 171
Cdd:TIGR02135  82 IKISSDLERIGDYAVNIAKRALRlKEEDAKPKHLEELEKMGKLALKMLKDALDAFLNKDAELARQVAEMDERVDELYRQI 161
                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|.
gi 16131592   172 VRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVKGQDF 222
Cdd:TIGR02135 162 FRELVTYMIEDPTNIEAALDVLLIARYLERIGDHATNIAERVIYIKTGKIV 212
NptA COG1283
Na+/phosphate symporter [Inorganic ion transport and metabolism];
18-211 3.83e-20

Na+/phosphate symporter [Inorganic ion transport and metabolism];


Pssm-ID: 440894 [Multi-domain]  Cd Length: 540  Bit Score: 88.70  E-value: 3.83e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  18 LESIRTQVMTMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIiAKRQPTASDLRLVMVISKTIA 97
Cdd:COG1283 335 LAQARREVLRMGDLVEEMLRRALELLREGDEKLAEEIRKLEDAVDRLEREIKLYLAKL-SRESLSEEESRRLAELLHIAN 413
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  98 ELERIGDVADKICRTALEKFSQQHQ---PLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVRQ 174
Cdd:COG1283 414 DLERIGDIIENLAELAEKKIEKGLEfseEGLEELRELHERVLENLRLALSALASGDLELARKLLEEKEEIRRLERELRKR 493
                       170       180       190
                ....*....|....*....|....*....|....*..
gi 16131592 175 LMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICE 211
Cdd:COG1283 494 HLERLREGRCSIETGSLHLDLLRDLERINDHLANIAY 530
PhoU pfam01895
PhoU domain; This family contains phosphate regulatory proteins including PhoU. PhoU proteins ...
28-111 5.60e-19

PhoU domain; This family contains phosphate regulatory proteins including PhoU. PhoU proteins are known to play a role in the regulation of phosphate uptake. The PhoU domain is composed of a three helix bundle. The PhoU protein contains two copies of this domain. The domain binds to an iron cluster via its conserved E/DXXXD motif.


Pssm-ID: 426500 [Multi-domain]  Cd Length: 87  Bit Score: 78.54  E-value: 5.60e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592    28 MGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERIGDVAD 107
Cdd:pfam01895   2 MGELVEEMLDDALEALENRDVELAREVIELDDEIDRLYLEIERELLLLLALQQPLASDLRLIIALLKIARDLERIGDHAV 81

                  ....
gi 16131592   108 KICR 111
Cdd:pfam01895  82 NIAE 85
PhoU pfam01895
PhoU domain; This family contains phosphate regulatory proteins including PhoU. PhoU proteins ...
131-213 2.92e-16

PhoU domain; This family contains phosphate regulatory proteins including PhoU. PhoU proteins are known to play a role in the regulation of phosphate uptake. The PhoU domain is composed of a three helix bundle. The PhoU protein contains two copies of this domain. The domain binds to an iron cluster via its conserved E/DXXXD motif.


Pssm-ID: 426500 [Multi-domain]  Cd Length: 87  Bit Score: 71.22  E-value: 2.92e-16
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592   131 LGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVRQLMTYMMEDS---RTIPSVLTALFCARSIERIGDRCQ 207
Cdd:pfam01895   2 MGELVEEMLDDALEALENRDVELAREVIELDDEIDRLYLEIERELLLLLALQQplaSDLRLIIALLKIARDLERIGDHAV 81

                  ....*.
gi 16131592   208 NICEFI 213
Cdd:pfam01895  82 NIAERV 87
NptA COG1283
Na+/phosphate symporter [Inorganic ion transport and metabolism];
131-235 3.99e-08

Na+/phosphate symporter [Inorganic ion transport and metabolism];


Pssm-ID: 440894 [Multi-domain]  Cd Length: 540  Bit Score: 53.26  E-value: 3.99e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592 131 LGRHTIQMLHDVLDAFARMDIDEAVRIYREDKKVDQEYEGIVR---QLMTYMM--EDSRTIPSVLTAlfcARSIERIGDR 205
Cdd:COG1283 345 MGDLVEEMLRRALELLREGDEKLAEEIRKLEDAVDRLEREIKLylaKLSRESLseEESRRLAELLHI---ANDLERIGDI 421
                        90       100       110
                ....*....|....*....|....*....|.
gi 16131592 206 CQNICEFI-FYYVKGQDFRHVGGDELDKLLA 235
Cdd:COG1283 422 IENLAELAeKKIEKGLEFSEEGLEELRELHE 452
NptA COG1283
Na+/phosphate symporter [Inorganic ion transport and metabolism];
27-115 5.10e-03

Na+/phosphate symporter [Inorganic ion transport and metabolism];


Pssm-ID: 440894 [Multi-domain]  Cd Length: 540  Bit Score: 37.85  E-value: 5.10e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131592  27 TMGGMVEQQLSDAITAMHNQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVIsktIAELERIGDVA 106
Cdd:COG1283 449 ELHERVLENLRLALSALASGDLELARKLLEEKEEIRRLERELRKRHLERLREGRCSIETGSLHLDL---LRDLERINDHL 525

                ....*....
gi 16131592 107 DKICRTALE 115
Cdd:COG1283 526 ANIAYPVLE 534
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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