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Conserved domains on  [gi|16131815|ref|NP_418412|]
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50S ribosomal subunit protein L10 [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

50S ribosomal protein L10( domain architecture ID 10011232)

50S ribosomal protein L10 binds and anchors the two L7/L12 dimers to the large ribosomal subunit

Gene Ontology:  GO:0006412|GO:0015934|GO:0070180
PubMed:  15989950|28524878

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
rplJ PRK00099
50S ribosomal protein L10; Reviewed
 3-164 1.68e-68

50S ribosomal protein L10; Reviewed


:

Pssm-ID: 234632  Cd Length: 172  Bit Score: 205.37  E-value: 1.68e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815    3 LNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLI 82
Cdd:PRK00099   1 MNREEKKEIVAELAEKLKKAQSAVVADYRGLTVAQMTELRKKLREAGVEYKVVKNTLARRALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   83 AYSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKE----------ASAGKLV 152
Cdd:PRK00099  81 AFSYEDPVAAAKVLKDFAKDNKKLEIKGGAIEGKVLDAEEVKALAKLPSREELLAKLLGVLQApatklagvlnAPPSKLA 160

                        170
                 ....*....|..
gi 16131815  153 RTLAAVRDAKEA 164
Cdd:PRK00099 161 RVLKALAEKKEA 172
 
Name Accession Description Interval E-value
rplJ PRK00099
50S ribosomal protein L10; Reviewed
 3-164 1.68e-68

50S ribosomal protein L10; Reviewed


Pssm-ID: 234632  Cd Length: 172  Bit Score: 205.37  E-value: 1.68e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815    3 LNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLI 82
Cdd:PRK00099   1 MNREEKKEIVAELAEKLKKAQSAVVADYRGLTVAQMTELRKKLREAGVEYKVVKNTLARRALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   83 AYSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKE----------ASAGKLV 152
Cdd:PRK00099  81 AFSYEDPVAAAKVLKDFAKDNKKLEIKGGAIEGKVLDAEEVKALAKLPSREELLAKLLGVLQApatklagvlnAPPSKLA 160

                        170
                 ....*....|..
gi 16131815  153 RTLAAVRDAKEA 164
Cdd:PRK00099 161 RVLKALAEKKEA 172
RplJ COG0244
Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 ...
 3-164 6.18e-64

Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 is part of the Pathway/BioSystem: Ribosome 50S subunit


Pssm-ID: 440014  Cd Length: 172  Bit Score: 193.74  E-value: 6.18e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   3 LNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLI 82
Cdd:COG0244   1 MTREEKKAVVAELKEKLKEAQAVVLTDYRGLTVAQMTELRRKLREAGVEYKVVKNTLARIALEGTGFEGLDDLLKGPTAI 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815  83 AYSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMK----------EASAGKLV 152
Cdd:COG0244  81 AFSYEDPVAAAKVLKDFAKENEKLVIKGGVLDGKVLDAEQVKALAKLPSREELLAKLLGLLQapatklvgvlNAPPRKLA 160

                       170
                ....*....|..
gi 16131815 153 RTLAAVRDAKEA 164
Cdd:COG0244 161 RVLKAIAEKKEA 172
Ribosomal_L10 cd05797
Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and ...
 4-160 1.59e-63

Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and eukaryotic mitochondrial 39S ribosomal protein, L10. L10 occupies the L7/L12 stalk of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These bacteria and eukaryotic sequences have no additional C-terminal domain, present in other eukaryotic and archaeal orthologs.


Pssm-ID: 240223  Cd Length: 157  Bit Score: 191.95  E-value: 1.59e-63
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   4 NLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIA 83
Cdd:cd05797   1 NREKKEEIVAELKEKLKEAKSVVVADYRGLTVAQLTELRKELREAGVKLKVVKNTLAKRALEGTGFEDLDDLLKGPTAIA 80

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 16131815  84 YSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKeASAGKLVRTLAAVRD 160
Cdd:cd05797  81 FSEEDPVAAAKVLKDFAKENKKLEIKGGVVEGKVLDAEEVKALAKLPSREELLAQLLGLLQ-APATKLVRVLNAPAS 156
Ribosomal_L10 pfam00466
Ribosomal protein L10;
4-101 4.74e-33

Ribosomal protein L10;


Pssm-ID: 459822 [Multi-domain]  Cd Length: 99  Bit Score: 112.64  E-value: 4.74e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815     4 NLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIA 83
Cdd:pfam00466   2 TREKKEELVEELKELLKEYKSVVVVDYRGLTVAQLTELRKKLRENGAELKVGKNTLMRRALEETGEEKLEDYLKGPTALL 81

                          90
                  ....*....|....*...
gi 16131815    84 YSMEHPGAAARLFKEFAK 101
Cdd:pfam00466  82 FTNEDPVAVAKVLEDFAK 99
 
Name Accession Description Interval E-value
rplJ PRK00099
50S ribosomal protein L10; Reviewed
 3-164 1.68e-68

50S ribosomal protein L10; Reviewed


Pssm-ID: 234632  Cd Length: 172  Bit Score: 205.37  E-value: 1.68e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815    3 LNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLI 82
Cdd:PRK00099   1 MNREEKKEIVAELAEKLKKAQSAVVADYRGLTVAQMTELRKKLREAGVEYKVVKNTLARRALEGTGFEGLDDLLKGPTAI 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   83 AYSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKE----------ASAGKLV 152
Cdd:PRK00099  81 AFSYEDPVAAAKVLKDFAKDNKKLEIKGGAIEGKVLDAEEVKALAKLPSREELLAKLLGVLQApatklagvlnAPPSKLA 160

                        170
                 ....*....|..
gi 16131815  153 RTLAAVRDAKEA 164
Cdd:PRK00099 161 RVLKALAEKKEA 172
RplJ COG0244
Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 ...
 3-164 6.18e-64

Ribosomal protein L10 [Translation, ribosomal structure and biogenesis]; Ribosomal protein L10 is part of the Pathway/BioSystem: Ribosome 50S subunit


Pssm-ID: 440014  Cd Length: 172  Bit Score: 193.74  E-value: 6.18e-64
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   3 LNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLI 82
Cdd:COG0244   1 MTREEKKAVVAELKEKLKEAQAVVLTDYRGLTVAQMTELRRKLREAGVEYKVVKNTLARIALEGTGFEGLDDLLKGPTAI 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815  83 AYSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMK----------EASAGKLV 152
Cdd:COG0244  81 AFSYEDPVAAAKVLKDFAKENEKLVIKGGVLDGKVLDAEQVKALAKLPSREELLAKLLGLLQapatklvgvlNAPPRKLA 160

                       170
                ....*....|..
gi 16131815 153 RTLAAVRDAKEA 164
Cdd:COG0244 161 RVLKAIAEKKEA 172
Ribosomal_L10 cd05797
Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and ...
 4-160 1.59e-63

Ribosomal protein L10 family, L10 subfamily; composed of bacterial 50S ribosomal protein and eukaryotic mitochondrial 39S ribosomal protein, L10. L10 occupies the L7/L12 stalk of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These bacteria and eukaryotic sequences have no additional C-terminal domain, present in other eukaryotic and archaeal orthologs.


Pssm-ID: 240223  Cd Length: 157  Bit Score: 191.95  E-value: 1.59e-63
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   4 NLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIA 83
Cdd:cd05797   1 NREKKEEIVAELKEKLKEAKSVVVADYRGLTVAQLTELRKELREAGVKLKVVKNTLAKRALEGTGFEDLDDLLKGPTAIA 80

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 16131815  84 YSMEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKeASAGKLVRTLAAVRD 160
Cdd:cd05797  81 FSEEDPVAAAKVLKDFAKENKKLEIKGGVVEGKVLDAEEVKALAKLPSREELLAQLLGLLQ-APATKLVRVLNAPAS 156
Ribosomal_L10_P0 cd00379
Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in ...
 6-160 6.63e-53

Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in bacteria, P0 in eukaryotes, and L10e in archaea, as well as uncharacterized P0-like eukaryotic proteins. In all three kingdoms, L10 forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). Some eukaryotic P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 238222 [Multi-domain]  Cd Length: 155  Bit Score: 165.05  E-value: 6.63e-53
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   6 QDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYS 85
Cdd:cd00379   1 EKKEELVEELKELLKKYKSVVVVDYRGLTVAQLTELRKELRESGAKLKVGKNTLMRRALKGTGFEELKPLLKGPTALAFT 80

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 16131815  86 MEHPGAAARLFKEFAKANAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKeASAGKLVRTLAAVRD 160
Cdd:cd00379  81 NEDPVEVAKVLKDFAKENKKLFAKGGVVAGKVLDPAGVTALAKLPSREELLAMLIGLLK-APIAKLARLLNALGI 154
Ribosomal_L10 pfam00466
Ribosomal protein L10;
4-101 4.74e-33

Ribosomal protein L10;


Pssm-ID: 459822 [Multi-domain]  Cd Length: 99  Bit Score: 112.64  E-value: 4.74e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815     4 NLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIA 83
Cdd:pfam00466   2 TREKKEELVEELKELLKEYKSVVVVDYRGLTVAQLTELRKKLRENGAELKVGKNTLMRRALEETGEEKLEDYLKGPTALL 81

                          90
                  ....*....|....*...
gi 16131815    84 YSMEHPGAAARLFKEFAK 101
Cdd:pfam00466  82 FTNEDPVAVAKVLEDFAK 99
rplP0 PRK04019
acidic ribosomal protein P0; Validated
 8-119 1.23e-04

acidic ribosomal protein P0; Validated


Pssm-ID: 179712 [Multi-domain]  Cd Length: 330  Bit Score: 41.01  E-value: 1.23e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815    8 KQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVyMRVVRNTLLRRAVEGTP---FECLKDAFVGPTLIAY 84
Cdd:PRK04019   8 KKEEVEELKELIKSYPVVGIVDLEGIPARQLQEIRRKLRGKAE-LKVSKNTLIKRALEEAGeedLEKLEDYLEGQVALIF 86

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 16131815   85 SMEHPgaaARLFKEFakanAKFEVKAAAFEGELIP 119
Cdd:PRK04019  87 TNMNP---FKLYKLL----EKSKTPAPAKPGDIAP 114
Ribosomal_P0_L10e cd05795
Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal ...
 6-119 6.33e-03

Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal protein P0 and the archaeal P0 homolog, L10e. P0 or L10e forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These eukaryotic and archaeal P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 240221 [Multi-domain]  Cd Length: 175  Bit Score: 35.63  E-value: 6.33e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16131815   6 QDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVyMRVVRNTLLRRAVEG-----TPFECLKDAFVGPT 80
Cdd:cd05795   1 EWKKEYVEKLTELLKSYPKVLIVDADNVGSKQLQKIRRSLRGKAE-ILMGKNTLIRRALRNlgdenPELEKLLPYLKGNV 79

                        90       100       110
                ....*....|....*....|....*....|....*....
gi 16131815  81 LIAYSMEHPgaaARLFKEFakanAKFEVKAAAFEGELIP 119
Cdd:cd05795  80 GFIFTNGDP---FEIRKIL----EENKVPAPAKPGAIAP 111
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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