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Conserved domains on  [gi|16132081|ref|NP_418680|]
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DNA polymerase III subunit chi [Escherichia coli str. K-12 substr. MG1655]

Protein Classification

DNA polymerase III subunit chi( domain architecture ID 10012572)

DNA polymerase III subunit chi dimerizes with subunit psi and binds to single-strand binding (SSB) protein and acts as a bridge between the DnaX clamp loader complex and the SSB

EC:  2.7.7.7
SCOP:  4001070

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PRK05728 PRK05728
DNA polymerase III subunit chi; Validated
1-147 8.09e-68

DNA polymerase III subunit chi; Validated


:

Pssm-ID: 235581  Cd Length: 142  Bit Score: 201.60  E-value: 8.09e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081    1 MKNATFYLLDndttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPV 80
Cdd:PRK05728   1 MTRADFYHLT-------LSALEALLCELAEKALRAGWRVLVQCEDEEQAEALDEALWTFRDESFLPHGLAGEGPAAGQPV 73
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 16132081   81 EIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK 147
Cdd:PRK05728  74 LLTWPGKRNANHRDLLINLDGAVPAFAAAFERVVDFVGYDEAAKQAARERWKAYRAAGYALTYWQQG 140
 
Name Accession Description Interval E-value
PRK05728 PRK05728
DNA polymerase III subunit chi; Validated
1-147 8.09e-68

DNA polymerase III subunit chi; Validated


Pssm-ID: 235581  Cd Length: 142  Bit Score: 201.60  E-value: 8.09e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081    1 MKNATFYLLDndttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPV 80
Cdd:PRK05728   1 MTRADFYHLT-------LSALEALLCELAEKALRAGWRVLVQCEDEEQAEALDEALWTFRDESFLPHGLAGEGPAAGQPV 73
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 16132081   81 EIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK 147
Cdd:PRK05728  74 LLTWPGKRNANHRDLLINLDGAVPAFAAAFERVVDFVGYDEAAKQAARERWKAYRAAGYALTYWQQG 140
HolC COG2927
DNA polymerase III, chi subunit [Replication, recombination and repair];
1-143 8.30e-57

DNA polymerase III, chi subunit [Replication, recombination and repair];


Pssm-ID: 442171  Cd Length: 136  Bit Score: 173.47  E-value: 8.30e-57
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081   1 MKNATFYLLDNdttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPV 80
Cdd:COG2927   1 MTRVDFYHLTR------VSDREALACRLAEKALRAGWRVLVLCPDEEQAEALDELLWTFRPDSFLPHGLAGEGLAADQPV 74
                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 16132081  81 EIAWPQKRsSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNT 143
Cdd:COG2927  75 LLTTDEEN-PNGADVLINLDGAVPDFFSRFERVIEIVDGDEEAKQAARERWKAYRAAGYELTY 136
DNA_pol3_chi pfam04364
DNA polymerase III chi subunit, HolC; The DNA polymerase III holoenzyme (EC:2.7.7.7) is the ...
1-141 3.74e-53

DNA polymerase III chi subunit, HolC; The DNA polymerase III holoenzyme (EC:2.7.7.7) is the polymerase responsible for the replication of the Escherichia coli chromosome. The holoenzyme is composed of the DNA polymerase III core, the sliding clamp, and the DnaX clamp loading complex. The DnaX complex contains either either the tau or gamma product of gene dnax, complexed to delta.delta' and to chi psi. Chi forms a 1:1 heterodimer with psi. The chi psi complex functions by increasing the affinity of tau and gamma for delta.delta' allowing a functional clamp-loading complex to form at physiological subunit concentrations. Psi is responsible for the interaction with DnaX (gamma/tau), but psi is insoluble unless it is in a complex with chi.


Pssm-ID: 461276 [Multi-domain]  Cd Length: 135  Bit Score: 164.27  E-value: 3.74e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081     1 MKNATFYLLDndttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGA-P 79
Cdd:pfam04364   1 MTRVDFYHLT-------RADREALACRLAEKALRQGWRVLVLCPDEEQAEALDELLWTFRPDSFLPHGLAGEGDALAAqP 73
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 16132081    80 VEIAWPQkRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNL 141
Cdd:pfam04364  74 VLLTTDG-LPEDGADVLINLADEVPPFFSRFERVIEFVDGDDEAKAAARERWKAYRARGYEL 134
 
Name Accession Description Interval E-value
PRK05728 PRK05728
DNA polymerase III subunit chi; Validated
1-147 8.09e-68

DNA polymerase III subunit chi; Validated


Pssm-ID: 235581  Cd Length: 142  Bit Score: 201.60  E-value: 8.09e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081    1 MKNATFYLLDndttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPV 80
Cdd:PRK05728   1 MTRADFYHLT-------LSALEALLCELAEKALRAGWRVLVQCEDEEQAEALDEALWTFRDESFLPHGLAGEGPAAGQPV 73
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 16132081   81 EIAWPQKRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK 147
Cdd:PRK05728  74 LLTWPGKRNANHRDLLINLDGAVPAFAAAFERVVDFVGYDEAAKQAARERWKAYRAAGYALTYWQQG 140
HolC COG2927
DNA polymerase III, chi subunit [Replication, recombination and repair];
1-143 8.30e-57

DNA polymerase III, chi subunit [Replication, recombination and repair];


Pssm-ID: 442171  Cd Length: 136  Bit Score: 173.47  E-value: 8.30e-57
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081   1 MKNATFYLLDNdttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPV 80
Cdd:COG2927   1 MTRVDFYHLTR------VSDREALACRLAEKALRAGWRVLVLCPDEEQAEALDELLWTFRPDSFLPHGLAGEGLAADQPV 74
                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 16132081  81 EIAWPQKRsSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNT 143
Cdd:COG2927  75 LLTTDEEN-PNGADVLINLDGAVPDFFSRFERVIEIVDGDEEAKQAARERWKAYRAAGYELTY 136
DNA_pol3_chi pfam04364
DNA polymerase III chi subunit, HolC; The DNA polymerase III holoenzyme (EC:2.7.7.7) is the ...
1-141 3.74e-53

DNA polymerase III chi subunit, HolC; The DNA polymerase III holoenzyme (EC:2.7.7.7) is the polymerase responsible for the replication of the Escherichia coli chromosome. The holoenzyme is composed of the DNA polymerase III core, the sliding clamp, and the DnaX clamp loading complex. The DnaX complex contains either either the tau or gamma product of gene dnax, complexed to delta.delta' and to chi psi. Chi forms a 1:1 heterodimer with psi. The chi psi complex functions by increasing the affinity of tau and gamma for delta.delta' allowing a functional clamp-loading complex to form at physiological subunit concentrations. Psi is responsible for the interaction with DnaX (gamma/tau), but psi is insoluble unless it is in a complex with chi.


Pssm-ID: 461276 [Multi-domain]  Cd Length: 135  Bit Score: 164.27  E-value: 3.74e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 16132081     1 MKNATFYLLDndttvdgLSAVEQLVCEIAAERWRSGKRVLIACEDEKQAYRLDEALWARPAESFVPHNLAGEGPRGGA-P 79
Cdd:pfam04364   1 MTRVDFYHLT-------RADREALACRLAEKALRQGWRVLVLCPDEEQAEALDELLWTFRPDSFLPHGLAGEGDALAAqP 73
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 16132081    80 VEIAWPQkRSSSRRDILISLRTSFADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNL 141
Cdd:pfam04364  74 VLLTTDG-LPEDGADVLINLADEVPPFFSRFERVIEFVDGDDEAKAAARERWKAYRARGYEL 134
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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