MATH domain; This motif has been called the Meprin And TRAF-Homology (MATH) domain. This domain is hugely expanded in the nematode C. elegans.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812   14 VKNISRFVDDESYFTDTEERFNIPWRIEIKKKDGNFELFLKCQKEECENRKWSIETEYTLKLVSQNGKCLMKNNKYTFEN 93
Cdd:pfam00917   1 IKNFSKIKEGESYYSPVEERFNIPWRLQIYRKGGFLGLYLHCDKEEELERGWSIETEFTLKLVSSNGKSVTKTDTHVFEK 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 115533812   94 QLGRGWE-FVSWKELESEYVVDDSIVVEAHVKI 125
Cdd:pfam00917  81 PKGWGWGkFISWDDLEKDYLVDDSITVEAHVKI 113

MATH domain; This motif has been called the Meprin And TRAF-Homology (MATH) domain. This domain is hugely expanded in the nematode C. elegans.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812   14 VKNISRFVDDESYFTDTEERFNIPWRIEIKKKDGNFELFLKCQKEECENRKWSIETEYTLKLVSQNGKCLMKNNKYTFEN 93
Cdd:pfam00917   1 IKNFSKIKEGESYYSPVEERFNIPWRLQIYRKGGFLGLYLHCDKEEELERGWSIETEFTLKLVSSNGKSVTKTDTHVFEK 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 115533812   94 QLGRGWE-FVSWKELESEYVVDDSIVVEAHVKI 125
Cdd:pfam00917  81 PKGWGWGkFISWDDLEKDYLVDDSITVEAHVKI 113

meprin and TRAF homology;

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812    10 INETVKNISRFVDDESYFTDTEERFNIPWRIEIKKKDGNFELFLKCQKEECENRKWSIETEYTLKLVSQNGKCLMKNNKY 89
Cdd:smart00061   2 LSHTFKNVSRLEEGESYFSPSEEHFNIPWRLKIYRKNGFLSLYLHCEKEECDSRKWSIEAEFTLKLVSQNGKSLSKKDKH 81

                           90
                   ....*....|
gi 115533812    90 TFENQLGRGW 99
Cdd:smart00061  82 VFEKPSGWGF 91

MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane and are capable of cleaving growth factors, extracellular matrix proteins, and biologically active peptides. TRAF molecules serve as adapter proteins that link cell surface receptors of the Tumor Necrosis Factor and 1nterleukin-1/Toll-like families to downstream kinase cascades, which results in the activation of transcription factors and the regulation of cell survival, proliferation and stress responses in the immune and inflammatory systems. Other members include the ubiquitin ligases, TRIM37 and SPOP, and the ubiquitin-specific proteases, HAUSP and Ubp21p. A large number of uncharacterized members mostly from lineage-specific expansions in C. elegans and rice contain MATH and BTB domains, similar to SPOP. The MATH domain has been shown to bind peptide/protein substrates in TRAFs and HAUSP. It is possible that the MATH domain in other members of this superfamily also interacts with various protein substrates. The TRAF domain may also be involved in the trimerization of TRAFs. Based on homology, it is postulated that the MATH domain in meprins may be involved in its tetramer assembly and that the MATH domain, in general, may take part in diverse modular arrangements defined by adjacent multimerization domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812  13 TVKNISRFV-DDESYFTDTEERFNIPWRIEIKKKDGN-----FELFLKCQKEECENRKWSIETEYTLKLVSQN-GKCLMK 85
Cdd:cd00121    4 TWKIVNFSElEGESIYSPPFEVGGYKWRIRIYPNGDGesgdyLSLYLELDKGESDLEKWSVRAEFTLKLVNQNgGKSLSK 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 115533812  86 NNKYTFENQLGRGW---EFVSWKELESEY-VVDDSIVVEAHVK 124
Cdd:cd00121   84 SFTHVFFSEKGSGWgfpKFISWDDLEDSYyLVDDSLTIEVEVK 126

MATH domain; This motif has been called the Meprin And TRAF-Homology (MATH) domain. This domain is hugely expanded in the nematode C. elegans.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812   14 VKNISRFVDDESYFTDTEERFNIPWRIEIKKKDGNFELFLKCQKEECENRKWSIETEYTLKLVSQNGKCLMKNNKYTFEN 93
Cdd:pfam00917   1 IKNFSKIKEGESYYSPVEERFNIPWRLQIYRKGGFLGLYLHCDKEEELERGWSIETEFTLKLVSSNGKSVTKTDTHVFEK 80

                          90       100       110
                  ....*....|....*....|....*....|...
gi 115533812   94 QLGRGWE-FVSWKELESEYVVDDSIVVEAHVKI 125
Cdd:pfam00917  81 PKGWGWGkFISWDDLEKDYLVDDSITVEAHVKI 113

meprin and TRAF homology;

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812    10 INETVKNISRFVDDESYFTDTEERFNIPWRIEIKKKDGNFELFLKCQKEECENRKWSIETEYTLKLVSQNGKCLMKNNKY 89
Cdd:smart00061   2 LSHTFKNVSRLEEGESYFSPSEEHFNIPWRLKIYRKNGFLSLYLHCEKEECDSRKWSIEAEFTLKLVSQNGKSLSKKDKH 81

                           90
                   ....*....|
gi 115533812    90 TFENQLGRGW 99
Cdd:smart00061  82 VFEKPSGWGF 91

MATH (meprin and TRAF-C homology) domain; an independent folding unit with an eight-stranded beta-sandwich structure found in meprins, TRAFs and other proteins. Meprins comprise a class of extracellular metalloproteases which are anchored to the membrane and are capable of cleaving growth factors, extracellular matrix proteins, and biologically active peptides. TRAF molecules serve as adapter proteins that link cell surface receptors of the Tumor Necrosis Factor and 1nterleukin-1/Toll-like families to downstream kinase cascades, which results in the activation of transcription factors and the regulation of cell survival, proliferation and stress responses in the immune and inflammatory systems. Other members include the ubiquitin ligases, TRIM37 and SPOP, and the ubiquitin-specific proteases, HAUSP and Ubp21p. A large number of uncharacterized members mostly from lineage-specific expansions in C. elegans and rice contain MATH and BTB domains, similar to SPOP. The MATH domain has been shown to bind peptide/protein substrates in TRAFs and HAUSP. It is possible that the MATH domain in other members of this superfamily also interacts with various protein substrates. The TRAF domain may also be involved in the trimerization of TRAFs. Based on homology, it is postulated that the MATH domain in meprins may be involved in its tetramer assembly and that the MATH domain, in general, may take part in diverse modular arrangements defined by adjacent multimerization domains.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812  13 TVKNISRFV-DDESYFTDTEERFNIPWRIEIKKKDGN-----FELFLKCQKEECENRKWSIETEYTLKLVSQN-GKCLMK 85
Cdd:cd00121    4 TWKIVNFSElEGESIYSPPFEVGGYKWRIRIYPNGDGesgdyLSLYLELDKGESDLEKWSVRAEFTLKLVNQNgGKSLSK 83

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 115533812  86 NNKYTFENQLGRGW---EFVSWKELESEY-VVDDSIVVEAHVK 124
Cdd:cd00121   84 SFTHVFFSEKGSGWgfpKFISWDDLEDSYyLVDDSLTIEVEVK 126

Herpesvirus-associated ubiquitin-specific protease (HAUSP, also known as USP7) family, N-terminal MATH (TRAF-like) domain; composed of proteins similar to human HAUSP, an enzyme that specifically catalyzes the deubiquitylation of p53 and MDM2, hence playing an important role in the p53-MDM2 pathway. It contains an N-terminal TRAF-like domain and a C-terminal catalytic protease (C19 family) domain. The tumor suppressor p53 protein is a transcription factor that responds to many cellular stress signals and is regulated primarily through ubiquitylation and subsequent degradation. MDM2 is a RING-finger E3 ubiquitin ligase that promotes p53 ubiquitinylation. p53 and MDM2 bind to the same site in the N-terminal TRAF-like domain of HAUSP in a mutually exclusive manner. HAUSP also interacts with the Epstein-Barr nuclear antigen 1 (EBNA1) protein of the Epstein-Barr virus (EBV), which efficiently immortalizes infected cells predisposing the host to a variety of cancers. EBNA1 plays several important roles in EBV latent infection and cellular transformation. It binds the same pocket as p53 in the HAUSP TRAF-like domain. Through interactions with p53, MDM2 and EBNA1, HAUSP plays a role in cell proliferation, apoptosis and EBV-mediated immortalization.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115533812  13 TVKNISRFvdDESYFTDTEERFNIPWRIEI--------KKKDGNFELFLKCQKEEcENRKWSIETEYTLKLVS--QNGKC 82
Cdd:cd03772    8 TVERFSRL--SESVLSPPCFVRNLPWKIMVmprnypdrNPHQKSVGFFLQCNAES-DSTSWSCHAQAVLRIINykDDEPS 84

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 115533812  83 LMKNNKYTF---ENQLGRGwEFVSWKEL---ESEYVVDDSIVVEAHVK 124
Cdd:cd03772   85 FSRRISHLFfskENDWGFS-NFMTWSEVtdpEKGFIEDDTITLEVYVQ 131