RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 418 PGAGEFRLQDfcTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDE-LRSKTVPNVGLHDVEVITDTLKR 496
Cdd:cd04382   1 IGTGELADFD--PSTSPMIPALIVHCVNEIEARGLTEEGLYRVSGSEREVKALKEKfLRGKTVPNLSKVDIHVICGCLKD 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 497 FLRDLKDPLIPRTSRQELIVAANLYstDPDNGRLALNRVICELPQANRDTLAYLFIHWRKViAQSSRNKMNCEAMARMVA 576
Cdd:cd04382  79 FLRSLKEPLITFALWKEFMEAAEIL--DEDNSRAALYQAISELPQPNRDTLAFLILHLQRV-AQSPECKMDINNLARVFG 155

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 17552778 577 PAVMGHPVKQSqSQAIAGRDATDCHRAMTALFEFDDVYW 615
Cdd:cd04382 156 PTIVGYSVPNP-DPMTILQDTVRQPRVVERLLEIPSDYW 193

Predicted nucleic acid-binding protein DR0291, contains C4-type Zn-ribbon domain [General function prediction only];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778  40 LIDEIERLRKLWKDSEESKKRLNADMREAEEALAKARKKLAMfdidVKdTQKHLRALMEENKALKLDLNVYETREKQLKD 119
Cdd:COG1579  43 LEARLEAAKTELEDLEKEIKRLELEIEEVEARIKKYEEQLGN----VR-NNKEYEALQKEIESLKRRISDLEDEILELME 117

                .
gi 17552778 120 A 120
Cdd:COG1579 118 R 118

RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 418 PGAGEFRLQDfcTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDE-LRSKTVPNVGLHDVEVITDTLKR 496
Cdd:cd04382   1 IGTGELADFD--PSTSPMIPALIVHCVNEIEARGLTEEGLYRVSGSEREVKALKEKfLRGKTVPNLSKVDIHVICGCLKD 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 497 FLRDLKDPLIPRTSRQELIVAANLYstDPDNGRLALNRVICELPQANRDTLAYLFIHWRKViAQSSRNKMNCEAMARMVA 576
Cdd:cd04382  79 FLRSLKEPLITFALWKEFMEAAEIL--DEDNSRAALYQAISELPQPNRDTLAFLILHLQRV-AQSPECKMDINNLARVFG 155

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 17552778 577 PAVMGHPVKQSqSQAIAGRDATDCHRAMTALFEFDDVYW 615
Cdd:cd04382 156 PTIVGYSVPNP-DPMTILQDTVRQPRVVERLLEIPSDYW 193

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778   437 PAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRS--KTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQEL 514
Cdd:pfam00620   1 PLIVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRgpDVDLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEEF 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778   515 IVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPV 584
Cdd:pfam00620  81 IEAAKL--PDEEERLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDVNKMNAHNLAIVFGPTLLRPPD 148

Phorbol esters/diacylglycerol binding domain (C1 domain); This domain is also known as the Protein kinase C conserved region 1 (C1) domain.

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 17552778   352 HTFIEAGIKAMRKCDKCATAL--KLATSMKCRDCHQVVHRSCCNKLHLPCIPR 402
Cdd:pfam00130   1 HHFVHRNFKQPTFCDHCGEFLwgLGKQGLKCSWCKLNVHKRCHEKVPPECGCD 53

RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 418 PGAGEFRLQDfcTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDE-LRSKTVPNVGLHDVEVITDTLKR 496
Cdd:cd04382   1 IGTGELADFD--PSTSPMIPALIVHCVNEIEARGLTEEGLYRVSGSEREVKALKEKfLRGKTVPNLSKVDIHVICGCLKD 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 497 FLRDLKDPLIPRTSRQELIVAANLYstDPDNGRLALNRVICELPQANRDTLAYLFIHWRKViAQSSRNKMNCEAMARMVA 576
Cdd:cd04382  79 FLRSLKEPLITFALWKEFMEAAEIL--DEDNSRAALYQAISELPQPNRDTLAFLILHLQRV-AQSPECKMDINNLARVFG 155

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 17552778 577 PAVMGHPVKQSqSQAIAGRDATDCHRAMTALFEFDDVYW 615
Cdd:cd04382 156 PTIVGYSVPNP-DPMTILQDTVRQPRVVERLLEIPSDYW 193

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778   437 PAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRS--KTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQEL 514
Cdd:pfam00620   1 PLIVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRgpDVDLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEEF 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778   515 IVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPV 584
Cdd:pfam00620  81 IEAAKL--PDEEERLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDVNKMNAHNLAIVFGPTLLRPPD 148

RhoGAP_fRGD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 435 MIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDEL------RSKTVPNVGLHDVEVITDTLKRFLRDLKDPLIPR 508
Cdd:cd04398  15 NVPNIVYQCIQAIENFGLNLEGIYRLSGNVSRVNKLKELFdkdplnVLLISPEDYESDIHSVASLLKLFFRELPEPLLTK 94

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 17552778 509 TSRQELIVAANlySTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHP 583
Cdd:cd04398  95 ALSREFIEAAK--IEDESRRRDALHGLINDLPDANYATLRALMFHLARIKEHESVNRMSVNNLAIIWGPTLMNAA 167

RhoGAP_ARHGAP27_15_12_9: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP27 (also called CAMGAP1), ARHGAP15, 12 and 9-like proteins; This subgroup of ARHGAPs are multidomain proteins that contain RhoGAP, PH, SH3 and WW domains. Most members that are studied show GAP activity towards Rac1, some additionally show activity towards Cdc42. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 425 LQDFCTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVL---LDELRSKTVPNVGLHDVEVITDTLKRFLRDL 501
Cdd:cd04403   5 LEALCQRENSTVPKFVRLCIEAVEKRGLDVDGIYRVSGNLAVIQKLrfaVDHDEKLDLDDSKWEDIHVITGALKLFFREL 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 502 KDPLIPRTSRQELIVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMg 581
Cdd:cd04403  85 PEPLFPYSLFNDFVAAIKL--SDYEQRVSAVKDLIKSLPKPNHDTLKMLFRHLCRVIEHGEKNRMTTQNLAIVFGPTLL- 161

                       170
                ....*....|.
gi 17552778 582 HPVKQSQSQAI 592
Cdd:cd04403 162 RPEQETGNIAV 172

RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ArfGAP activity is PIP3-dependent, ARAPs are considered integration points for phosphoinositide, Arf and Rho signaling. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 436 IPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTV---PNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQ 512
Cdd:cd04385  15 IPVIVDKCIDFITQHGLMSEGIYRKNGKNSSVKKLLEAFRKDARsvqLREGEYTVHDVADVLKRFLRDLPDPLLTSELHA 94

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 17552778 513 ELIVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVM 580
Cdd:cd04385  95 EWIEAAEL--ENKDERIARYKELIRRLPPINRATLKVLIGHLYRVQKHSDENQMSVHNLALVFGPTLF 160

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 434 PMIPAAVIHCVVALEarGLTQEGIYRVPGQVRTVNVLLDELRSKTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQE 513
Cdd:cd04389  22 PWILTFLSEKVLALG--GFQTEGIFRVPGDIDEVNELKLRVDQWDYPLSGLEDPHVPASLLKLWLRELEEPLIPDALYQQ 99

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 17552778 514 LIVAANlystDPDNGRlalnRVICELPQANRDTLAYLfIHWRKVIAQS---SRNKMNCEAMARMVAP 577
Cdd:cd04389 100 CISASE----DPDKAV----EIVQKLPIINRLVLCYL-INFLQVFAQPenvAHTKMDVSNLAMVFAP 157

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 425 LQDFCTSAKPmIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLD--------ELRSKTVPnvglhdVEVITDTLKR 496
Cdd:cd04373   5 LANVVTSEKP-IPIFLEKCVEFIEATGLETEGIYRVSGNKTHLDSLQKqfdqdhnlDLVSKDFT------VNAVAGALKS 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 497 FLRDLKDPLIPRTSRQELIVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVA 576
Cdd:cd04373  78 FFSELPDPLIPYSMHLELVEAAKI--NDREQRLHALKELLKKFPPENFDVFKYVITHLNKVSQNSKVNLMTSENLSICFW 155

                ....
gi 17552778 577 PAVM 580
Cdd:cd04373 156 PTLM 159

RhoGAP_ARHGAP18: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP18-like proteins. The function of ArhGAP18 is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 447 LEARGLTQEGIYRVPGQVRTVNVLLDELRSK------TVPNVGLHDVEVItdtLKRFLRDLKDPLIPrtsrQELIVAANL 520
Cdd:cd04391  33 LEERGLETEGILRIPGSAQRVKFLCQELEAKfyegtfLWDQVKQHDAASL---LKLFIRELPQPLLT----VEYLPAFYS 105

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 521 YSTDPDNGRL--ALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMG----HPvKQSQSQAIAG 594
Cdd:cd04391 106 VQGLPSKKDQlqALNLLVLLLPEANRDTLKALLEFLQKVVDHEEKNKMNLWNVAMIMAPNLFPprgkHS-KDNESLQEEV 184

                       170       180
                ....*....|....*....|..
gi 17552778 595 RDATDCHRAMTALFEFDDVYWQ 616
Cdd:cd04391 185 NMAAGCANIMRLLIRYQDLLWT 206

RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for Rac1 and Cdc42. Short isoforms (without the PH domain) of ARHGAP24, called RC-GAP72 and p73RhoGAP, and of ARHGAP22, called p68RacGAP, has been shown to be involved in angiogenesis and endothelial cell capillary formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 435 MIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTVPNV-GLHDVEVITDTLKRFLRDLKDPLIPRTSRQE 513
Cdd:cd04390  21 LVPILVEQCVDFIREHGLKEEGLFRLPGQANLVKQLQDAFDAGERPSFdSDTDVHTVASLLKLYLRELPEPVIPWAQYED 100

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 17552778 514 LIVAANLYSTDPDNGRLALNRVICELPQANRDTLAYL--FIHwrKVIAQSSRNKMNCEAMARMVAP 577
Cdd:cd04390 101 FLSCAQLLSKDEEKGLGELMKQVSILPKVNYNLLSYIcrFLD--EVQSNSSVNKMSVQNLATVFGP 164

RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 425 LQDFCTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPG---QVRTVNVLLDELRSKTVPNVGLHDVEVITDTLKRFLRDL 501
Cdd:cd04386   9 LEEHLKRTGREIALPIEACVMCLLETGMNEEGLFRVGGgasKLKRLKAALDAGTFSLPLDEFYSDPHAVASALKSYLREL 88

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 502 KDPLIPRTSRQELIVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMG 581
Cdd:cd04386  89 PDPLLTYNLYEDWVQAANK--PDEDERLQAIWRILNKLPRENRDNLRYLIKFLSKLAQKSDENKMSPSNIAIVLAPNLLW 166

                       170
                ....*....|....*..
gi 17552778 582 HPVKQSQSQAIAGRDAT 598
Cdd:cd04386 167 AKNEGSLAEMAAGTSVH 183

RhoGAP_Graf: GTPase-activator protein (GAP) domain for Rho-like GTPases found in GRAF (GTPase regulator associated with focal adhesion kinase); Graf is a multi-domain protein, containing SH3 and PH domains, that binds focal adhesion kinase and influences cytoskeletal changes mediated by Rho proteins. Graf exhibits GAP activity toward RhoA and Cdc42, but only weakly activates Rac1. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 443 CVVALEARGLTQEGIYRVPG---QV-RTVNVLLDElRSKTVPNVGLH----DVEVITDTLKRFLRDLKDPLIPRTSRQEL 514
Cdd:cd04374  35 CIEAVETRGINEQGLYRVVGvnsKVqKLLSLGLDP-KTSTPGDVDLDnsewEIKTITSALKTYLRNLPEPLMTYELHNDF 113

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 17552778 515 IVAANlySTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVM 580
Cdd:cd04374 114 INAAK--SENLESRVNAIHSLVHKLPEKNREMLELLIKHLTNVSDHSKKNLMTVSNLGVVFGPTLL 177

RhoGAP_myosin_IXB: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 430 TSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTvPNVGLHD--VEVITDTLKRFLRDLKDPLIP 507
Cdd:cd04407   9 TSNKTSVPIVLEKLLEHVEMHGLYTEGIYRKSGSANRMKELHQLLQADP-ENVKLENypIHAITGLLKQWLRELPEPLMT 87

                        90       100       110       120       130       140       150
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 17552778 508 RTSRQELIVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHP 583
Cdd:cd04407  88 FAQYNDFLRAVEL--PEKQEQLQAIYRVLEQLPTANHNTLERLIFHLVKVALEEDVNRMSPNALAIVFAPCLLRCP 161

RhoGAP_GMIP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 436 IPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRS-KTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQEL 514
Cdd:cd04408  16 VPFVVVRCTAEIENRALGVQGIYRISGSKARVEKLCQAFENgRDLVDLSGHSPHDITSVLKHFLKELPEPVLPFQLYDDF 95

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 515 IVAANLYSTDPD----------NGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPV 584
Cdd:cd04408  96 IALAKELQRDSEkaaespsiveNIIRSLKELLGRLPVSNYNTLRHLMAHLYRVAERFEDNKMSPNNLGIVFGPTLLRPLV 175

                ....*
gi 17552778 585 KQSQS 589
Cdd:cd04408 176 GGDVS 180

RhoGAP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of PARG1 (PTPL1-associated RhoGAP1). PARG1 was originally cloned as an interaction partner of PTPL1, an intracellular protein-tyrosine phosphatase. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 427 DFCTSAKPM---IPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSktvpnvGLHDVEV-------ITDTLKR 496
Cdd:cd04409   4 DFAQVAKKSpdgIPFIIKKCTSEIESRALCLKGIYRVNGAKSRVEKLCQAFEN------GKDLVELselsphdISNVLKL 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 497 FLRDLKDPLIPRTSRQELIVAAN--LYSTDPDNGRLA-----------LNRVIC-------ELPQANRDTLAYLFIHWRK 556
Cdd:cd04409  78 YLRQLPEPLILFRLYNEFIGLAKesQHVNETQEAKKNsdkkwpnmcteLNRILLkskdllrQLPAPNYNTLQFLIVHLHR 157

                       170       180
                ....*....|....*....|....
gi 17552778 557 VIAQSSRNKMNCEAMARMVAPAVM 580
Cdd:cd04409 158 VSEQAEENKMSASNLGIIFGPTLI 181

RhoGAP-ARHGAP11A: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP11A-like proteins. The mouse homolog of human ArhGAP11A has been detected as a gene exclusively expressed in immature ganglion cells, potentially playing a role in retinal development. The exact function of ArhGAP11A is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 436 IPAAVIHCVVALEARgLTQEGIYRVPGQVRTVNVLLDELRSKTVPNVGLHDVEViTDTLKRFLRDLKDPLIPrTSRQELI 515
Cdd:cd04394  20 VPKFLVDACTFLLDH-LSTEGLFRKSGSVVRQKELKAKLEGGEACLSSALPCDV-AGLLKQFFRELPEPLLP-YDLHEAL 96

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 17552778 516 VAANLYSTDPDNGRlALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVM 580
Cdd:cd04394  97 LKAQELPTDEERKS-ATLLLTCLLPDEHVNTLRYFFSFLYDVAQRCSENKMDSSNLAVIFAPNLF 160

RhoGAP-p50rhoGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p50RhoGAP-like proteins; p50RhoGAP, also known as RhoGAP-1, contains a C-terminal RhoGAP domain and an N-terminal Sec14 domain which binds phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). It is ubiquitously expressed and preferentially active on Cdc42. This subgroup also contains closely related ARHGAP8. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 435 MIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDEL-RSKTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQE 513
Cdd:cd04404  22 PIPPVVRETVEYLQAHALTTEGIFRRSANTQVVKEVQQKYnMGEPVDFDQYEDVHLPAVILKTFLRELPEPLLTFDLYDD 101

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|...
gi 17552778 514 LivaANLYSTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKM 566
Cdd:cd04404 102 I---VGFLNVDKEERVERVKQLLQTLPEENYQVLKYLIKFLVQVSAHSDQNKM 151

RhoGAP_myosin_IXA: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXA. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 430 TSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKtVPNVGLHD--VEVITDTLKRFLRDLKDPLIP 507
Cdd:cd04406   9 TSEDRSVPLVVEKLINYIEMHGLYTEGIYRKSGSTNKIKELRQGLDTD-ANSVNLDDynIHVIASVFKQWLRDLPNPLMT 87

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 508 RTSRQELIVAANLysTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPVKQS 587
Cdd:cd04406  88 FELYEEFLRAMGL--QERRETVRGVYSVIDQLSRTHLNTLERLIFHLVRIALQEETNRMSANALAIVFAPCILRCPDTTD 165

                ....*
gi 17552778 588 QSQAI 592
Cdd:cd04406 166 PLQSV 170

RhoGAP_srGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an N-terminal FCH domain, a central RhoGAP domain and a C-terminal SH3 domain; this SH3 domain interacts with the intracellular proline-rich-tail of the Roundabout receptor (Robo). This interaction with Robo then activates the rhoGAP domain which in turn inhibits Cdc42 activity. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 17552778 424 RLQDFCTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTVPNVGL---HDVEVITDTLKRFLRD 500
Cdd:cd04383   6 SLEEYIQDSGQAIPLVVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNAFERGEDPLADDqndHDINSVAGVLKLYFRG 85