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Conserved domains on  [gi|71991703|ref|NP_499967|]
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Protein sleepless [Caenorhabditis elegans]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TFP super family cl45932
three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU ...
 19-111 2.29e-04

three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU (also known as Ly-6 antigen/uPA receptor)-like extracellular domain (ECD) occurs singly in GPI-linked cell-surface glycoproteins (Ly-6 family, CD59, thymocyte B cell antigen, Sgp-2) or as three-fold repeated domain in urokinase-type plasminogen activator receptor. It is a structural domain involved in protein-protein interactions, tolerating an unusual degree of variation and binding with high specificity to a broad spectrum of targets. The snake toxin domain is present in short and long neurotoxins, cytotoxins, and short toxins, and in other miscellaneous venom peptides. The toxin acts by binding to the nicotinic acetylcholine receptors in the postsynaptic membrane of skeletal muscles and preventing the binding of acetylcholine, thereby blocking the excitation of muscles. Both the LU-like ECD and the snake toxin domain belong to three-fingered protein (TFP) fold, which is characterized by containing 70 to 100 amino acids including eight to ten cysteine residues spaced at conserved distances.


The actual alignment was detected with superfamily member cd23592:

Pssm-ID: 480272  Cd Length: 104  Bit Score: 37.86  E-value: 2.29e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703  19 CYICNSKKQPDC---IDNYQAFNTIC----PVRSLGGVKlhePVGCRVTRQYSKEKMWIIRECGYLGE------ERE-RK 84
Cdd:cd23592   5 CWVCRSDYDPRCgdpFDNLTLPITDCnqekKPHHLPGVK---ATMCRKMRQKVNGEWRYIRSCAFLGEpgiggdERWcLE 81

                        90       100
                ....*....|....*....|....*..
gi 71991703  85 FNTTTYLNGEPATCVfsqcSENLCNSA 111
Cdd:cd23592  82 RSGTYNIHIEDCTCN----SKDGCNAA 104
 
Name Accession Description Interval E-value
TFP_LU_ECD_Crok cd23592
extracellular domain (ECD), Ly6/uPAR superfamily, three finger domain (TFD) fold, found in ...
 19-111 2.29e-04

extracellular domain (ECD), Ly6/uPAR superfamily, three finger domain (TFD) fold, found in Drosophila melanogaster protein crooked and similar proteins; Crooked (Crok) is a glycosylphosphatidylinositol (GPI)-anchored protein that plays an essential role in septa formation, the membrane accumulation of SJ components and paracellular barrier functions. It required for septate junction (SJ) formation and function in a tissue-autonomous manner. Crooked is specifically required for correct membrane trafficking of Neurexin IV, a central SJ component. Crooked is a member of the Drosophila Ly6 superfamily. It contains an extracellular domain (ECD), which belongs to Ly-6 antigen/uPA receptor-like (LU) superfamily and exhibits a snake toxin-like fold (also known as three-finger toxin/3FTx fold or three-fingered protein/TFP domain fold).


Pssm-ID: 467121  Cd Length: 104  Bit Score: 37.86  E-value: 2.29e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703  19 CYICNSKKQPDC---IDNYQAFNTIC----PVRSLGGVKlhePVGCRVTRQYSKEKMWIIRECGYLGE------ERE-RK 84
Cdd:cd23592   5 CWVCRSDYDPRCgdpFDNLTLPITDCnqekKPHHLPGVK---ATMCRKMRQKVNGEWRYIRSCAFLGEpgiggdERWcLE 81

                        90       100
                ....*....|....*....|....*..
gi 71991703  85 FNTTTYLNGEPATCVfsqcSENLCNSA 111
Cdd:cd23592  82 RSGTYNIHIEDCTCN----SKDGCNAA 104
QVR pfam17064
Sleepless protein; In Drosophila QUIVER (also known as SLEEPLESS protein) is required for ...
 17-109 3.05e-03

Sleepless protein; In Drosophila QUIVER (also known as SLEEPLESS protein) is required for homoeostatic regulation of sleep under normal conditions and following sleep deprivation. It is a novel potassium channel subunit that modulates the Shaker potassium channel which regulates the sleep.


Pssm-ID: 435716  Cd Length: 85  Bit Score: 34.50  E-value: 3.05e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703    17 LNCYICNSKKQPDCIDNYQAFNTICPVRSLGGvklhepvGCR-VTRQYSKEKMWIIRECG-YLGEERERKFNTTTYLNGE 94
Cdd:pfam17064   1 IKCYSCNSSDDPGCGDPFPFNSSSIKLVDCDG-------GCVkIKTKGSGGSTRVTRGCGpELTEDIKDGCSSSSSGGGG 73

                          90
                  ....*....|....*
gi 71991703    95 PATCvfsQCSENLCN 109
Cdd:pfam17064  74 TITC---FCNTDLCN 85
 
Name Accession Description Interval E-value
TFP_LU_ECD_Crok cd23592
extracellular domain (ECD), Ly6/uPAR superfamily, three finger domain (TFD) fold, found in ...
 19-111 2.29e-04

extracellular domain (ECD), Ly6/uPAR superfamily, three finger domain (TFD) fold, found in Drosophila melanogaster protein crooked and similar proteins; Crooked (Crok) is a glycosylphosphatidylinositol (GPI)-anchored protein that plays an essential role in septa formation, the membrane accumulation of SJ components and paracellular barrier functions. It required for septate junction (SJ) formation and function in a tissue-autonomous manner. Crooked is specifically required for correct membrane trafficking of Neurexin IV, a central SJ component. Crooked is a member of the Drosophila Ly6 superfamily. It contains an extracellular domain (ECD), which belongs to Ly-6 antigen/uPA receptor-like (LU) superfamily and exhibits a snake toxin-like fold (also known as three-finger toxin/3FTx fold or three-fingered protein/TFP domain fold).


Pssm-ID: 467121  Cd Length: 104  Bit Score: 37.86  E-value: 2.29e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703  19 CYICNSKKQPDC---IDNYQAFNTIC----PVRSLGGVKlhePVGCRVTRQYSKEKMWIIRECGYLGE------ERE-RK 84
Cdd:cd23592   5 CWVCRSDYDPRCgdpFDNLTLPITDCnqekKPHHLPGVK---ATMCRKMRQKVNGEWRYIRSCAFLGEpgiggdERWcLE 81

                        90       100
                ....*....|....*....|....*..
gi 71991703  85 FNTTTYLNGEPATCVfsqcSENLCNSA 111
Cdd:cd23592  82 RSGTYNIHIEDCTCN----SKDGCNAA 104
QVR pfam17064
Sleepless protein; In Drosophila QUIVER (also known as SLEEPLESS protein) is required for ...
 17-109 3.05e-03

Sleepless protein; In Drosophila QUIVER (also known as SLEEPLESS protein) is required for homoeostatic regulation of sleep under normal conditions and following sleep deprivation. It is a novel potassium channel subunit that modulates the Shaker potassium channel which regulates the sleep.


Pssm-ID: 435716  Cd Length: 85  Bit Score: 34.50  E-value: 3.05e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703    17 LNCYICNSKKQPDCIDNYQAFNTICPVRSLGGvklhepvGCR-VTRQYSKEKMWIIRECG-YLGEERERKFNTTTYLNGE 94
Cdd:pfam17064   1 IKCYSCNSSDDPGCGDPFPFNSSSIKLVDCDG-------GCVkIKTKGSGGSTRVTRGCGpELTEDIKDGCSSSSSGGGG 73

                          90
                  ....*....|....*
gi 71991703    95 PATCvfsQCSENLCN 109
Cdd:pfam17064  74 TITC---FCNTDLCN 85
TFP_LU_ECD_Twit cd23593
extracellular domain (ECD) found in Drosophila melanogaster Target of wit (Twit) and similar ...
 17-111 6.26e-03

extracellular domain (ECD) found in Drosophila melanogaster Target of wit (Twit) and similar proteins; Twit is a GPI-anchored membrane ligand of the Ly6 family that participates in retrograde signalling as a downstream target of the bone morphogenetic protein (BMP) pathway receptor encoded by Wishful thinking (Wit). It acts in neurons, regulating spontaneous neurotransmitter release during the maturation of larval neuromuscular junctions. Twit contains an extracellular domain (ECD), which belongs to Ly-6 antigen/uPA receptor-like (LU) superfamily and exhibits a snake toxin-like fold (also known as three-finger toxin/3FTx fold or three-fingered protein/TFP domain fold).


Pssm-ID: 467122  Cd Length: 101  Bit Score: 33.92  E-value: 6.26e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703  17 LNCYICNSKKQPDCIDNYQAFNTI-----CPVRSLGGVKLHE-PVgCRVTRQYSKEKMWIIRECGYLGEER-ERKFNTTT 89
Cdd:cd23593   2 LRCWSCSSDTDPFCGDPFNTTENHfhltdCDRTASPSYYLNErPV-CRKIVQRVNGELVVIRSCAWENEDDvDGPCSNTS 80

                        90       100
                ....*....|....*....|...
gi 71991703  90 ylNGEPATCVF-SQCSENLCNSA 111
Cdd:cd23593  81 --TGSYIKIEScETCSTDGCNSA 101
TFP cd00117
three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU ...
 17-109 8.78e-03

three-fingered protein (TFP) fold found in Ly6/uPAR (LU) and snake toxin superfamily; The LU (also known as Ly-6 antigen/uPA receptor)-like extracellular domain (ECD) occurs singly in GPI-linked cell-surface glycoproteins (Ly-6 family, CD59, thymocyte B cell antigen, Sgp-2) or as three-fold repeated domain in urokinase-type plasminogen activator receptor. It is a structural domain involved in protein-protein interactions, tolerating an unusual degree of variation and binding with high specificity to a broad spectrum of targets. The snake toxin domain is present in short and long neurotoxins, cytotoxins, and short toxins, and in other miscellaneous venom peptides. The toxin acts by binding to the nicotinic acetylcholine receptors in the postsynaptic membrane of skeletal muscles and preventing the binding of acetylcholine, thereby blocking the excitation of muscles. Both the LU-like ECD and the snake toxin domain belong to three-fingered protein (TFP) fold, which is characterized by containing 70 to 100 amino acids including eight to ten cysteine residues spaced at conserved distances.


Pssm-ID: 467060  Cd Length: 81  Bit Score: 33.23  E-value: 8.78e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71991703  17 LNCYICNSKKQPDCiDNYQAFNTICPVRSlggvklhepVGCRVTRQYSKEKMW-IIRECGYLGEERErkfNTTTYLNGEP 95
Cdd:cd00117   1 LKCYQCNSSNDPNC-CNSSPTLVTCSSPE---------TFCRKIVGKVGGGETlVIRGCATECECGC---TECCSGTGTS 67

                        90
                ....*....|....
gi 71991703  96 ATCVFSQCSENLCN 109
Cdd:cd00117  68 GTTCTSCCDTDLCN 81
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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