Frag1/DRAM/Sfk1 family; This family includes Frag1, DRAM and Sfk1 proteins. Frag1 (FGF receptor activating protein 1) is a protein that is conserved from fungi to humans. There are four potential iso-prenylation sites throughout the peptide, viz CILW, CIIW and CIGL. Frag1 is a membrane-spanning protein that is ubiquitously expressed in adult tissues suggesting an important cellular function. Dram is a family of proteins conserved from nematodes to humans with six hydrophobic transmembrane regions and an Endoplasmic Reticulum signal peptide. It is a lysosomal protein that induces macro-autophagy as an effector of p53-mediated death, where p53 is the tumour-suppressor gene that is frequently mutated in cancer. Expression of Dram is stress-induced. This region is also part of a family of small plasma membrane proteins, referred to as Sfk1, that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, thus allowing proper localization of Stt4p to the actin cytoskeleton.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71986291    52 CVFAHLVSYSAFMLMLIAYIRHRQTVEYYAHRlgWENSVWRLMSTIIAGIGFFAALCITVIAHCEDPqlaIVHDLADTIA 131
Cdd:pfam10277  55 CFFSIAINIGAFLRLAVAFLRYLRLRPLARRS--ERVLRLNILALVFGLLGALGLSLVSNFQSTEDH---SVHDIGAILF 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71986291   132 FSAGLLYAWSQAVLTWALVPR--MCTLKVCIFRFTIALIETVAFI----FYRFVKDDVNLSPPWLLEASLWVIAICFHVF 205
Cdd:pfam10277 130 FVFGFIYMLLQTALSYRLGPHytPKSRKSFRLKLVLLILAFVSAVafivFFIRHKKHCGYGAYSLSAIFEWLLALSFIFF 209

                  ....*....
gi 71986291   206 IITFFIELR 214
Cdd:pfam10277 210 FGTFAVDFK 218

Frag1/DRAM/Sfk1 family; This family includes Frag1, DRAM and Sfk1 proteins. Frag1 (FGF receptor activating protein 1) is a protein that is conserved from fungi to humans. There are four potential iso-prenylation sites throughout the peptide, viz CILW, CIIW and CIGL. Frag1 is a membrane-spanning protein that is ubiquitously expressed in adult tissues suggesting an important cellular function. Dram is a family of proteins conserved from nematodes to humans with six hydrophobic transmembrane regions and an Endoplasmic Reticulum signal peptide. It is a lysosomal protein that induces macro-autophagy as an effector of p53-mediated death, where p53 is the tumour-suppressor gene that is frequently mutated in cancer. Expression of Dram is stress-induced. This region is also part of a family of small plasma membrane proteins, referred to as Sfk1, that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, thus allowing proper localization of Stt4p to the actin cytoskeleton.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71986291    52 CVFAHLVSYSAFMLMLIAYIRHRQTVEYYAHRlgWENSVWRLMSTIIAGIGFFAALCITVIAHCEDPqlaIVHDLADTIA 131
Cdd:pfam10277  55 CFFSIAINIGAFLRLAVAFLRYLRLRPLARRS--ERVLRLNILALVFGLLGALGLSLVSNFQSTEDH---SVHDIGAILF 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71986291   132 FSAGLLYAWSQAVLTWALVPR--MCTLKVCIFRFTIALIETVAFI----FYRFVKDDVNLSPPWLLEASLWVIAICFHVF 205
Cdd:pfam10277 130 FVFGFIYMLLQTALSYRLGPHytPKSRKSFRLKLVLLILAFVSAVafivFFIRHKKHCGYGAYSLSAIFEWLLALSFIFF 209

                  ....*....
gi 71986291   206 IITFFIELR 214
Cdd:pfam10277 210 FGTFAVDFK 218

Frag1/DRAM/Sfk1 family; This family includes Frag1, DRAM and Sfk1 proteins. Frag1 (FGF receptor activating protein 1) is a protein that is conserved from fungi to humans. There are four potential iso-prenylation sites throughout the peptide, viz CILW, CIIW and CIGL. Frag1 is a membrane-spanning protein that is ubiquitously expressed in adult tissues suggesting an important cellular function. Dram is a family of proteins conserved from nematodes to humans with six hydrophobic transmembrane regions and an Endoplasmic Reticulum signal peptide. It is a lysosomal protein that induces macro-autophagy as an effector of p53-mediated death, where p53 is the tumour-suppressor gene that is frequently mutated in cancer. Expression of Dram is stress-induced. This region is also part of a family of small plasma membrane proteins, referred to as Sfk1, that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, thus allowing proper localization of Stt4p to the actin cytoskeleton.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71986291    52 CVFAHLVSYSAFMLMLIAYIRHRQTVEYYAHRlgWENSVWRLMSTIIAGIGFFAALCITVIAHCEDPqlaIVHDLADTIA 131
Cdd:pfam10277  55 CFFSIAINIGAFLRLAVAFLRYLRLRPLARRS--ERVLRLNILALVFGLLGALGLSLVSNFQSTEDH---SVHDIGAILF 129

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71986291   132 FSAGLLYAWSQAVLTWALVPR--MCTLKVCIFRFTIALIETVAFI----FYRFVKDDVNLSPPWLLEASLWVIAICFHVF 205
Cdd:pfam10277 130 FVFGFIYMLLQTALSYRLGPHytPKSRKSFRLKLVLLILAFVSAVafivFFIRHKKHCGYGAYSLSAIFEWLLALSFIFF 209

                  ....*....
gi 71986291   206 IITFFIELR 214
Cdd:pfam10277 210 FGTFAVDFK 218