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Conserved domains on  [gi|19112370|ref|NP_595578|]
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uncharacterized protein SPOM_SPBC8D2.16C [Schizosaccharomyces pombe]

Protein Classification

RNA methyltransferase( domain architecture ID 10495637)

SpoU family RNA methyltransferase catalyzes the methylation of rRNA or tRNA at specific sites in a S-adenosyl-L-methionine (SAM)-dependent manner; similar to Homo sapiens C9orf114

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Methyltrn_RNA_3 pfam02598
Putative RNA methyltransferase; This family has a TIM barrel-like fold with a deep C-terminal ...
17-305 6.91e-74

Putative RNA methyltransferase; This family has a TIM barrel-like fold with a deep C-terminal trefoil knot. The arrangement of its hydrophilic and hydrophobic surfaces are opposite to that of the classic TIM barrel proteins. It is likely to bind RNA, and may function as a methyltransferase.


:

Pssm-ID: 460611  Cd Length: 295  Bit Score: 229.39  E-value: 6.91e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370    17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEYVQNTQVHTLSSDAYLK--DPTKFLTDLLCYFET 94
Cdd:pfam02598   1 TVSIAIPSSILSNAQSLEQKTYLAGQIARAATIFNVDEIVVFDDSSGSERSKRTEPKSERKTGdsDPCEFLARLLQYLET 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370    95 PFFMRKELFPLNPHLKYTSCFPLLP----LRNDKastvniEFPYREGIVTHPSPQAKNK--YIINAGLSHNVIVSSPSVl 168
Cdd:pfam02598  81 PPYLRKSLFPIHPDLKYAGLLPPLDaphhLRNDE------WGPYREGVVIPKPSPGKVKktKYVNIGLKKPVSIELPEV- 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370   169 APRTRVTVRLKaqspnEEGQLQGDIVSFSAPREKGGHYWGYKVRSCLS-SQLCKSSPYKGGYDFVVQiNSQTSAITSKEL 247
Cdd:pfam02598 154 PPGTRVTVDLS-----SREPVRGKVVSPSEPREEAGYYWGYSVRLASSlSDVFTECPYPGGYDLSIG-TSERGKPVDSVL 227
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 19112370   248 EASLPSSFRHAVLV------LQDNVVQ-KELVDSTAKSIsFNpfpvSFLNDPI--------PEEILLAAMTRL 305
Cdd:pfam02598 228 PELSKISFKHLLIVfgglrgLEEAFKAdKELEGEDAREL-FD----GWLNTCPgqgsrtirTEEAVLIALALL 295
 
Name Accession Description Interval E-value
Methyltrn_RNA_3 pfam02598
Putative RNA methyltransferase; This family has a TIM barrel-like fold with a deep C-terminal ...
17-305 6.91e-74

Putative RNA methyltransferase; This family has a TIM barrel-like fold with a deep C-terminal trefoil knot. The arrangement of its hydrophilic and hydrophobic surfaces are opposite to that of the classic TIM barrel proteins. It is likely to bind RNA, and may function as a methyltransferase.


Pssm-ID: 460611  Cd Length: 295  Bit Score: 229.39  E-value: 6.91e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370    17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEYVQNTQVHTLSSDAYLK--DPTKFLTDLLCYFET 94
Cdd:pfam02598   1 TVSIAIPSSILSNAQSLEQKTYLAGQIARAATIFNVDEIVVFDDSSGSERSKRTEPKSERKTGdsDPCEFLARLLQYLET 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370    95 PFFMRKELFPLNPHLKYTSCFPLLP----LRNDKastvniEFPYREGIVTHPSPQAKNK--YIINAGLSHNVIVSSPSVl 168
Cdd:pfam02598  81 PPYLRKSLFPIHPDLKYAGLLPPLDaphhLRNDE------WGPYREGVVIPKPSPGKVKktKYVNIGLKKPVSIELPEV- 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370   169 APRTRVTVRLKaqspnEEGQLQGDIVSFSAPREKGGHYWGYKVRSCLS-SQLCKSSPYKGGYDFVVQiNSQTSAITSKEL 247
Cdd:pfam02598 154 PPGTRVTVDLS-----SREPVRGKVVSPSEPREEAGYYWGYSVRLASSlSDVFTECPYPGGYDLSIG-TSERGKPVDSVL 227
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 19112370   248 EASLPSSFRHAVLV------LQDNVVQ-KELVDSTAKSIsFNpfpvSFLNDPI--------PEEILLAAMTRL 305
Cdd:pfam02598 228 PELSKISFKHLLIVfgglrgLEEAFKAdKELEGEDAREL-FD----GWLNTCPgqgsrtirTEEAVLIALALL 295
HsC9orf114-like cd18086
SPOUT superfamily RNA methyltransferase HsC9orf114-like; Human C9orf114 (also known as ...
17-305 2.06e-35

SPOUT superfamily RNA methyltransferase HsC9orf114-like; Human C9orf114 (also known as centromere protein 32 or CENP-32) is required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. CENP-32 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349959  Cd Length: 187  Bit Score: 126.60  E-value: 2.06e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEYVQNTqvhtlssdAYLKDPTKFLTDLLCYFETPF 96
Cdd:cd18086   1 TLSIAIPSSILSNAQSLELKTYKAGQIARAAAIFRVDEIVVYDDSSASEDT--------KEKSDDAEFLARLLQYLETPP 72
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  97 FMRKELFPLnphlkytscfpllplrndkastvniefpyregivthpspqaknkyiinaglshnvivsspsvlaprtrvtv 176
Cdd:cd18086  73 YLRKRLFPK----------------------------------------------------------------------- 81
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370 177 rlkaqspneegqlqgdivsfsaprekgghYWGYKVRSCLSSQLCKSSPYKGGYDFVVQINSQTSAITSKELEASLPSSFR 256
Cdd:cd18086  82 -----------------------------YWGYSVRVAKSLSEVFTSPYKGGYDLVIGTSRRGEDISSVLLESILLPSFK 132
                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*
gi 19112370 257 HAVLV---LQDNVVQKELVDSTAKS---ISFNPFPVSFLNDPIPEEILLAAMTRL 305
Cdd:cd18086 133 HLLLVfggPDKGLEEALEADGDPKElfdHYVNTIPGQGSRTIRTEEALLITLALL 187
MTH1 COG2106
Predicted RNA methylase MTH1, SPOUT superfamily [General function prediction only];
17-214 4.76e-21

Predicted RNA methylase MTH1, SPOUT superfamily [General function prediction only];


Pssm-ID: 441709  Cd Length: 266  Bit Score: 90.31  E-value: 4.76e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEyvqntqvhtlssDAYLKDPtKFLTDLLCYFETPF 96
Cdd:COG2106   1 KLSVAIPSSLLSEEPDLREKTRKIGYIARAAAIFRVDRIVIYPDRE------------GERRRDG-EFIETVLRYLATPP 67
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  97 FMRKELFPLNPHLKYTSCFPllPLR----NDKASTVNIEfpYREGIVThpspQAKNKYIINAGLSHnvIVSSPSVLAPRT 172
Cdd:COG2106  68 YLRKRLFPLRDELRYAGVLP--PLRipshTGSKSPKVGD--LRQGLVT----RVDGKVRVDIGLGH--PISLPGKLPEGE 137
                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 19112370 173 RVTVRLKAQSPneegqLQGDIVsfsaprEKGGHYWGYKVRSC 214
Cdd:COG2106 138 RVTVRISSREP-----VRAEPV------DEPEIYWGYEVKRT 168
 
Name Accession Description Interval E-value
Methyltrn_RNA_3 pfam02598
Putative RNA methyltransferase; This family has a TIM barrel-like fold with a deep C-terminal ...
17-305 6.91e-74

Putative RNA methyltransferase; This family has a TIM barrel-like fold with a deep C-terminal trefoil knot. The arrangement of its hydrophilic and hydrophobic surfaces are opposite to that of the classic TIM barrel proteins. It is likely to bind RNA, and may function as a methyltransferase.


Pssm-ID: 460611  Cd Length: 295  Bit Score: 229.39  E-value: 6.91e-74
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370    17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEYVQNTQVHTLSSDAYLK--DPTKFLTDLLCYFET 94
Cdd:pfam02598   1 TVSIAIPSSILSNAQSLEQKTYLAGQIARAATIFNVDEIVVFDDSSGSERSKRTEPKSERKTGdsDPCEFLARLLQYLET 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370    95 PFFMRKELFPLNPHLKYTSCFPLLP----LRNDKastvniEFPYREGIVTHPSPQAKNK--YIINAGLSHNVIVSSPSVl 168
Cdd:pfam02598  81 PPYLRKSLFPIHPDLKYAGLLPPLDaphhLRNDE------WGPYREGVVIPKPSPGKVKktKYVNIGLKKPVSIELPEV- 153
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370   169 APRTRVTVRLKaqspnEEGQLQGDIVSFSAPREKGGHYWGYKVRSCLS-SQLCKSSPYKGGYDFVVQiNSQTSAITSKEL 247
Cdd:pfam02598 154 PPGTRVTVDLS-----SREPVRGKVVSPSEPREEAGYYWGYSVRLASSlSDVFTECPYPGGYDLSIG-TSERGKPVDSVL 227
                         250       260       270       280       290       300       310
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 19112370   248 EASLPSSFRHAVLV------LQDNVVQ-KELVDSTAKSIsFNpfpvSFLNDPI--------PEEILLAAMTRL 305
Cdd:pfam02598 228 PELSKISFKHLLIVfgglrgLEEAFKAdKELEGEDAREL-FD----GWLNTCPgqgsrtirTEEAVLIALALL 295
HsC9orf114-like cd18086
SPOUT superfamily RNA methyltransferase HsC9orf114-like; Human C9orf114 (also known as ...
17-305 2.06e-35

SPOUT superfamily RNA methyltransferase HsC9orf114-like; Human C9orf114 (also known as centromere protein 32 or CENP-32) is required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. CENP-32 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.


Pssm-ID: 349959  Cd Length: 187  Bit Score: 126.60  E-value: 2.06e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEYVQNTqvhtlssdAYLKDPTKFLTDLLCYFETPF 96
Cdd:cd18086   1 TLSIAIPSSILSNAQSLELKTYKAGQIARAAAIFRVDEIVVYDDSSASEDT--------KEKSDDAEFLARLLQYLETPP 72
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  97 FMRKELFPLnphlkytscfpllplrndkastvniefpyregivthpspqaknkyiinaglshnvivsspsvlaprtrvtv 176
Cdd:cd18086  73 YLRKRLFPK----------------------------------------------------------------------- 81
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370 177 rlkaqspneegqlqgdivsfsaprekgghYWGYKVRSCLSSQLCKSSPYKGGYDFVVQINSQTSAITSKELEASLPSSFR 256
Cdd:cd18086  82 -----------------------------YWGYSVRVAKSLSEVFTSPYKGGYDLVIGTSRRGEDISSVLLESILLPSFK 132
                       250       260       270       280       290
                ....*....|....*....|....*....|....*....|....*....|....*
gi 19112370 257 HAVLV---LQDNVVQKELVDSTAKS---ISFNPFPVSFLNDPIPEEILLAAMTRL 305
Cdd:cd18086 133 HLLLVfggPDKGLEEALEADGDPKElfdHYVNTIPGQGSRTIRTEEALLITLALL 187
MTH1 COG2106
Predicted RNA methylase MTH1, SPOUT superfamily [General function prediction only];
17-214 4.76e-21

Predicted RNA methylase MTH1, SPOUT superfamily [General function prediction only];


Pssm-ID: 441709  Cd Length: 266  Bit Score: 90.31  E-value: 4.76e-21
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  17 TISLALPISSLNVAYNLQLKTSFVWKISRIVSLYGIDEIILLEDPEyvqntqvhtlssDAYLKDPtKFLTDLLCYFETPF 96
Cdd:COG2106   1 KLSVAIPSSLLSEEPDLREKTRKIGYIARAAAIFRVDRIVIYPDRE------------GERRRDG-EFIETVLRYLATPP 67
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19112370  97 FMRKELFPLNPHLKYTSCFPllPLR----NDKASTVNIEfpYREGIVThpspQAKNKYIINAGLSHnvIVSSPSVLAPRT 172
Cdd:COG2106  68 YLRKRLFPLRDELRYAGVLP--PLRipshTGSKSPKVGD--LRQGLVT----RVDGKVRVDIGLGH--PISLPGKLPEGE 137
                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 19112370 173 RVTVRLKAQSPneegqLQGDIVsfsaprEKGGHYWGYKVRSC 214
Cdd:COG2106 138 RVTVRISSREP-----VRAEPV------DEPEIYWGYEVKRT 168
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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