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Conserved domains on  [gi|19921230|ref|NP_609619|]
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uncharacterized protein Dmel_CG16812 [Drosophila melanogaster]

Protein Classification

SAM domain-containing protein; ACK family non-receptor tyrosine-protein kinase( domain architecture ID 10175790)

SAM (sterile alpha motif) domain-containing protein may be involved in protein-protein interaction and in developmental regulation| ACK (activated Cdc42-associated kinase) family non-receptor tyrosine-protein kinase catalyzes the transfer of the gamma-phosphoryl group from ATP to tyrosine (tyr) residues in protein substrates; such as TNK1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SAM_CS047 cd09531
SAM domain of CS047 subfamily; SAM (sterile alpha motif) domain of CS047 subfamily is a ...
 6-70 4.25e-37

SAM domain of CS047 subfamily; SAM (sterile alpha motif) domain of CS047 subfamily is a putative protein-protein interaction domain. Proteins of this subfamily have a SAM domain at the N-terminus. SAM is a widespread domain in signaling and regulatory proteins. In many cases SAM mediates homodimerization/oligomerization. The exact function of proteins belonging to this group is unknown.


:

Pssm-ID: 188930 [Multi-domain]  Cd Length: 65  Bit Score: 130.11  E-value: 4.25e-37
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 19921230   6 AARWVKFFNAAGIPSPAAAGYAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSKTVC 70
Cdd:cd09531   1 TSEWIKFFKDAGIPSGLAAKYAVIFVENRIQKEMLLDLNKEILKEMGITVMGDIIAILKHAKKVH 65
DUF5577 super family cl39267
Family of unknown function (DUF5577); This is a family of unknown function found in Metazoa.
 109-215 3.16e-06

Family of unknown function (DUF5577); This is a family of unknown function found in Metazoa.


The actual alignment was detected with superfamily member pfam17740:

Pssm-ID: 407613  Cd Length: 334  Bit Score: 48.81  E-value: 3.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19921230   109 PAKPARRVLPEHEGKYKVTLPSGTTERSKQILAKREKlysdrVSSSKKSDIFTRLHAEdeaqegivsssgessvrvhvag 188
Cdd:pfam17740  68 PAAKHRRVTAEMEGKYIIHMPKGSTPRTRKILAQQQA-----AKGLHRTSVFARLGAE---------------------- 120

                          90       100       110
                  ....*....|....*....|....*....|
gi 19921230   189 aSKTAATSSNNS---VFARLggkqGALPEE 215
Cdd:pfam17740 121 -SKADTTTSGNKptgVFSRL----GAGDEE 145
 
Name Accession Description Interval E-value
SAM_CS047 cd09531
SAM domain of CS047 subfamily; SAM (sterile alpha motif) domain of CS047 subfamily is a ...
 6-70 4.25e-37

SAM domain of CS047 subfamily; SAM (sterile alpha motif) domain of CS047 subfamily is a putative protein-protein interaction domain. Proteins of this subfamily have a SAM domain at the N-terminus. SAM is a widespread domain in signaling and regulatory proteins. In many cases SAM mediates homodimerization/oligomerization. The exact function of proteins belonging to this group is unknown.


Pssm-ID: 188930 [Multi-domain]  Cd Length: 65  Bit Score: 130.11  E-value: 4.25e-37
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 19921230   6 AARWVKFFNAAGIPSPAAAGYAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSKTVC 70
Cdd:cd09531   1 TSEWIKFFKDAGIPSGLAAKYAVIFVENRIQKEMLLDLNKEILKEMGITVMGDIIAILKHAKKVH 65
SAM_4 pfam18017
SAM domain (Sterile alpha motif); This entry corresponds to a SAM domain that is found at the ...
 4-73 9.15e-23

SAM domain (Sterile alpha motif); This entry corresponds to a SAM domain that is found at the N-terminus of the human C19orf47 protein.


Pssm-ID: 407856 [Multi-domain]  Cd Length: 84  Bit Score: 91.67  E-value: 9.15e-23
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19921230     4 SSAARWVKFFNAAGIPSPAAAGYAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSKTVCEQN 73
Cdd:pfam18017   6 MATSEWIQFFKDAGIPAGLAVNYAVMFVDNRIQKNMLLDLNKDIMMDLGITVIGDIIAILKHAKQVHRQD 75
DUF5577 pfam17740
Family of unknown function (DUF5577); This is a family of unknown function found in Metazoa.
 109-215 3.16e-06

Family of unknown function (DUF5577); This is a family of unknown function found in Metazoa.


Pssm-ID: 407613  Cd Length: 334  Bit Score: 48.81  E-value: 3.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19921230   109 PAKPARRVLPEHEGKYKVTLPSGTTERSKQILAKREKlysdrVSSSKKSDIFTRLHAEdeaqegivsssgessvrvhvag 188
Cdd:pfam17740  68 PAAKHRRVTAEMEGKYIIHMPKGSTPRTRKILAQQQA-----AKGLHRTSVFARLGAE---------------------- 120

                          90       100       110
                  ....*....|....*....|....*....|
gi 19921230   189 aSKTAATSSNNS---VFARLggkqGALPEE 215
Cdd:pfam17740 121 -SKADTTTSGNKptgVFSRL----GAGDEE 145
SAM smart00454
Sterile alpha motif; Widespread domain in signalling and nuclear proteins. In EPH-related ...
 26-72 1.99e-03

Sterile alpha motif; Widespread domain in signalling and nuclear proteins. In EPH-related tyrosine kinases, appears to mediate cell-cell initiated signal transduction via the binding of SH2-containing proteins to a conserved tyrosine that is phosphorylated. In many cases mediates homodimerisation.


Pssm-ID: 197735  Cd Length: 68  Bit Score: 36.50  E-value: 1.99e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 19921230     26 YAHVFVENRIQDDMLLDLNK-EYLREMGITLMGDIIAILRHSKTVCEQ 72
Cdd:smart00454  21 YADNFRKNGIDGALLLLLTSeEDLKELGITKLGHRKKILKAIQKLKEQ 68
 
Name Accession Description Interval E-value
SAM_CS047 cd09531
SAM domain of CS047 subfamily; SAM (sterile alpha motif) domain of CS047 subfamily is a ...
 6-70 4.25e-37

SAM domain of CS047 subfamily; SAM (sterile alpha motif) domain of CS047 subfamily is a putative protein-protein interaction domain. Proteins of this subfamily have a SAM domain at the N-terminus. SAM is a widespread domain in signaling and regulatory proteins. In many cases SAM mediates homodimerization/oligomerization. The exact function of proteins belonging to this group is unknown.


Pssm-ID: 188930 [Multi-domain]  Cd Length: 65  Bit Score: 130.11  E-value: 4.25e-37
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 19921230   6 AARWVKFFNAAGIPSPAAAGYAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSKTVC 70
Cdd:cd09531   1 TSEWIKFFKDAGIPSGLAAKYAVIFVENRIQKEMLLDLNKEILKEMGITVMGDIIAILKHAKKVH 65
SAM_4 pfam18017
SAM domain (Sterile alpha motif); This entry corresponds to a SAM domain that is found at the ...
 4-73 9.15e-23

SAM domain (Sterile alpha motif); This entry corresponds to a SAM domain that is found at the N-terminus of the human C19orf47 protein.


Pssm-ID: 407856 [Multi-domain]  Cd Length: 84  Bit Score: 91.67  E-value: 9.15e-23
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19921230     4 SSAARWVKFFNAAGIPSPAAAGYAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSKTVCEQN 73
Cdd:pfam18017   6 MATSEWIQFFKDAGIPAGLAVNYAVMFVDNRIQKNMLLDLNKDIMMDLGITVIGDIIAILKHAKQVHRQD 75
SAM_Ste11_fungal cd09534
SAM domain of Ste11_fungal subfamily; SAM (sterile alpha motif) domain of Ste11 subfamily is a ...
 26-67 1.92e-07

SAM domain of Ste11_fungal subfamily; SAM (sterile alpha motif) domain of Ste11 subfamily is a protein-protein interaction domain. Proteins of this subfamily have SAM domain at the N-terminus and protein kinase domain at the C-terminus. They participate in regulation of mating pheromone response, invasive growth and high osmolarity growth response. MAP triple kinase Ste11 from S.cerevisia is known to interact with Ste20 kinase and Ste50 regulator. These kinases are able to form homodimers interacting through their SAM domains as well as heterodimers or heterogenous complexes when either SAM domain of monomeric or homodimeric form of Ste11 interacts with Ste50 regulator.


Pssm-ID: 188933  Cd Length: 62  Bit Score: 47.97  E-value: 1.92e-07
                        10        20        30        40
                ....*....|....*....|....*....|....*....|..
gi 19921230  26 YAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSK 67
Cdd:cd09534  18 YLDIFEKNLITGDLLLELDKEALKELGITKVGDRIRLLRAIK 59
SAM_superfamily cd09487
SAM (Sterile alpha motif ); SAM (Sterile Alpha Motif) domain is a module consisting of ...
 26-68 3.72e-07

SAM (Sterile alpha motif ); SAM (Sterile Alpha Motif) domain is a module consisting of approximately 70 amino acids. This domain is found in the Fungi/Metazoa group and in a restricted number of bacteria. Proteins with SAM domains are represented by a wide variety of domain architectures and have different intracellular localization, including nucleus, cytoplasm and membranes. SAM domains have diverse functions. They can interact with proteins, RNAs and membrane lipids, contain site of phosphorylation and/or kinase docking site, and play a role in protein homo and hetero dimerization/oligomerization in processes ranging from signal transduction to regulation of transcription. Mutations in SAM domains have been linked to several diseases.


Pssm-ID: 188886 [Multi-domain]  Cd Length: 56  Bit Score: 46.85  E-value: 3.72e-07
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
gi 19921230  26 YAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRHSKT 68
Cdd:cd09487  14 YADLFRKNEIDGDALLLLTDEDLKELGITSPGHRKKILRAIQR 56
DUF5577 pfam17740
Family of unknown function (DUF5577); This is a family of unknown function found in Metazoa.
 109-215 3.16e-06

Family of unknown function (DUF5577); This is a family of unknown function found in Metazoa.


Pssm-ID: 407613  Cd Length: 334  Bit Score: 48.81  E-value: 3.16e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 19921230   109 PAKPARRVLPEHEGKYKVTLPSGTTERSKQILAKREKlysdrVSSSKKSDIFTRLHAEdeaqegivsssgessvrvhvag 188
Cdd:pfam17740  68 PAAKHRRVTAEMEGKYIIHMPKGSTPRTRKILAQQQA-----AKGLHRTSVFARLGAE---------------------- 120

                          90       100       110
                  ....*....|....*....|....*....|
gi 19921230   189 aSKTAATSSNNS---VFARLggkqGALPEE 215
Cdd:pfam17740 121 -SKADTTTSGNKptgVFSRL----GAGDEE 145
SAM_SLA1_fungal cd09532
SAM domain of SLA1 subfamily; SAM (sterile alpha motif) domain of fungal SLA1 proteins is a ...
 9-65 4.11e-06

SAM domain of SLA1 subfamily; SAM (sterile alpha motif) domain of fungal SLA1 proteins is a protein-protein interaction domain. Proteins of this group consist of a few N-terminal SH3 domains followed by SHD1 domain, SAM domain (also known as SHD2) and multiple C-terminal repeats. The yeast SLA1 protein is an endocytic clathrin adaptor. It is associated with a variety of endocytic accessory factors and required for endocytic vesicle formation and for clathrin and actin-dependent cargo recognition. SLA1 binds clathrin through a variant clathrin-binding motif (vCB). The SAM domain negatively regulates this binding by blocking the vCB site. The SAM domains of SLA1 proteins can form oligomers via their mid-loop (ML) and end-helix (EH) regions. Such self-associations apparently are important for SLA1 function. A proposed regulatory model suggests that SAM can be considered a mediator of two aspects of clathrin adaptor function. It plays a role in negative regulation of clathrin binding via an intramolecular interaction with the vCB, and a role in positive regulation of vesicle coat assembly via self-oligomerization.


Pssm-ID: 188931 [Multi-domain]  Cd Length: 62  Bit Score: 43.93  E-value: 4.11e-06
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 19921230   9 WVKFFNAAGIPSPAAAGYAHVFVENRIQDDMLLDLNKEYLREMGITlMGDIIAILRH 65
Cdd:cd09532   3 WFEFFLNCGVDVSLCQRYASNFERDQMDESVLPDINPSTLRTLGLR-EGDIIRVMKF 58
SAM_Ste50-like_fungal cd09533
SAM domain of Ste50_like (ubc2) subfamily; SAM (sterile alpha motif) domain of Ste50-like (or ...
 26-64 9.24e-05

SAM domain of Ste50_like (ubc2) subfamily; SAM (sterile alpha motif) domain of Ste50-like (or Ubc2 for Ustilago bypass of cyclase) subfamily is a putative protein-protein interaction domain. This group includes only fungal proteins. Basidiomycetes have an N-terminal SAM domain, central UBQ domain, and C-terminal SH3 domain, while Ascomycetes lack the SH3 domain. Ubc2 of Ustilago maydis is a major virulence and maize pathogenicity factor. It is required for filamentous growth (the budding haploid form of Ustilago maydis is a saprophyte, while filamentous dikaryotic form is a pathogen). Also the Ubc2 protein is involved in the pheromone-responsive morphogenesis via the MAP kinase cascade. The SAM domain is necessary for ubc2 function; deletion of SAM eliminates this function. A Lys-to-Glu mutation in the SAM domain of ubc2 gene induces temperature sensitivity.


Pssm-ID: 188932  Cd Length: 58  Bit Score: 39.99  E-value: 9.24e-05
                        10        20        30
                ....*....|....*....|....*....|....*....
gi 19921230  26 YAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILR 64
Cdd:cd09533  14 YEDQFIENGITGDVLVALDHEDLKEMGITSVGHRLTILK 52
SAM_MLTK cd09529
SAM domain of MLTK subfamily; SAM (sterile alpha motif) domain of MLTK subfamily is a ...
 9-63 1.00e-04

SAM domain of MLTK subfamily; SAM (sterile alpha motif) domain of MLTK subfamily is a protein-protein interaction domain. Besides SAM domain, these proteins have N-terminal protein tyrosine kinase domain and leucine-zipper motif. Proteins of this group act as mitogen-activated protein triple kinase in a number of MAPK cascades. They can be activated by autophosphorylation in response to stress signals. MLTK-alpha is known to phosphorylate histone H3. In mammals, MLTKs participate in the activation of the JNK/SAPK, p38, ERK5 pathways, the transcriptional factor NF-kB, in the regulation of the cell cycle checkpoint, and in the induction of apoptosis in a hepatoma cell line. Some members of this subfamily are proto-oncogenes, thus MLTK-alpha is involved in neoplasmic cell transformation and/or skin cancer development in athymic nude mice. Based on in vivo coprecipitation experiments in mammalian cells, it has been demonstrated that MLTK proteins might form homodimers/oligomers via their SAM domains.


Pssm-ID: 188928  Cd Length: 71  Bit Score: 40.57  E-value: 1.00e-04
                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*.
gi 19921230   9 WVK-FFNAAGIPSPAAAgYAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAIL 63
Cdd:cd09529  10 WMQqLVRKGGHPSELSQ-YADLFKENHITGKRLLLLTEEDLRDMGIGSKGHIIHLK 64
SAM_1 pfam00536
SAM domain (Sterile alpha motif); It has been suggested that SAM is an evolutionarily ...
 26-65 7.98e-04

SAM domain (Sterile alpha motif); It has been suggested that SAM is an evolutionarily conserved protein binding domain that is involved in the regulation of numerous developmental processes in diverse eukaryotes. The SAM domain can potentially function as a protein interaction module through its ability to homo- and heterooligomerise with other SAM domains.


Pssm-ID: 425739  Cd Length: 64  Bit Score: 37.63  E-value: 7.98e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 19921230    26 YAHVFVENRIQDDMLLDLNKEYLREMGITLMGDIIAILRH 65
Cdd:pfam00536  20 YIDSFRAGYIDGDALLQLTEDDLLKLGVTLLGHRKKILYA 59
SAM smart00454
Sterile alpha motif; Widespread domain in signalling and nuclear proteins. In EPH-related ...
 26-72 1.99e-03

Sterile alpha motif; Widespread domain in signalling and nuclear proteins. In EPH-related tyrosine kinases, appears to mediate cell-cell initiated signal transduction via the binding of SH2-containing proteins to a conserved tyrosine that is phosphorylated. In many cases mediates homodimerisation.


Pssm-ID: 197735  Cd Length: 68  Bit Score: 36.50  E-value: 1.99e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*...
gi 19921230     26 YAHVFVENRIQDDMLLDLNK-EYLREMGITLMGDIIAILRHSKTVCEQ 72
Cdd:smart00454  21 YADNFRKNGIDGALLLLLTSeEDLKELGITKLGHRKKILKAIQKLKEQ 68
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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