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Conserved domains on  [gi|24656635|ref|NP_611540|]
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uncharacterized protein Dmel_CG15653 [Drosophila melanogaster]

Protein Classification

Coa1/Tim21 domain-containing protein( domain architecture ID 231040)

Coa1/Tim21 domain-containing protein such as mitochondrial import inner membrane translocase subunit Tim21, an essential component of the TIM23 complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane

CATH:  3.10.450.320
Gene Ontology:  GO:0098798|GO:0030150
PubMed:  17099692|15797382

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Coa1 super family cl21648
Cytochrome oxidase complex assembly protein 1; Coa1 is an inner mitochondrial membrane protein ...
 17-120 7.85e-11

Cytochrome oxidase complex assembly protein 1; Coa1 is an inner mitochondrial membrane protein that associates with Shy1 and is required for cytochrome oxidase complex IV assembly. It contains a conserved hydrophobic segment (amino acids 74-92) with the potential to form a membrane-spanning helix. The N-terminus of Coa1 is rich in positively charged amino acids and could form an amphipathic alpha helix, characteriztic of a mitochondrial presequence. A cleavage site for the mitochondrial processing peptidase is predicted adjacent to the presequence. Upon in vitro import into mitochondria, Coa1 is processed to a mature form, indicating that it possesses a cleavable presequence. The eukaryotic cytochrome oxidase complex consists of 12-13 subunits, with three mitochondrial encoded subunits, Cox1-Cox3, forming the core enzyme. Translation of the Cox1 transcript requires the two promoters, Pet309 and Mss51, and the latter has an additional role in translational elongation. Coa1 is necessary for linking the activity of Mss51 to Cox1 insertion into the assembly complex.


The actual alignment was detected with superfamily member pfam08695:

Pssm-ID: 451344  Cd Length: 117  Bit Score: 56.10  E-value: 7.85e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24656635    17 VYGAVASISAVMYMKWKMEDRVKSAEYYKLALKALRSHRGAVGLLGEPI-------KDSG--------IDLSnannncna 81
Cdd:pfam08695   2 VTFAIFVIGVGAALAYIFNYEKTSSPIYRSTLYQLRRSPEARELLGDNIpfksgwpWISGelntvqgrIDIS-------- 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 24656635    82 eearceVAVRGSKDKGTLYFWASNQPDRG-WLIDRLELET 120
Cdd:pfam08695  74 ------FPVKGSKGSGTVYLTATRESKRHpFHIHEWSLTV 107
 
Name Accession Description Interval E-value
Coa1 pfam08695
Cytochrome oxidase complex assembly protein 1; Coa1 is an inner mitochondrial membrane protein ...
 17-120 7.85e-11

Cytochrome oxidase complex assembly protein 1; Coa1 is an inner mitochondrial membrane protein that associates with Shy1 and is required for cytochrome oxidase complex IV assembly. It contains a conserved hydrophobic segment (amino acids 74-92) with the potential to form a membrane-spanning helix. The N-terminus of Coa1 is rich in positively charged amino acids and could form an amphipathic alpha helix, characteriztic of a mitochondrial presequence. A cleavage site for the mitochondrial processing peptidase is predicted adjacent to the presequence. Upon in vitro import into mitochondria, Coa1 is processed to a mature form, indicating that it possesses a cleavable presequence. The eukaryotic cytochrome oxidase complex consists of 12-13 subunits, with three mitochondrial encoded subunits, Cox1-Cox3, forming the core enzyme. Translation of the Cox1 transcript requires the two promoters, Pet309 and Mss51, and the latter has an additional role in translational elongation. Coa1 is necessary for linking the activity of Mss51 to Cox1 insertion into the assembly complex.


Pssm-ID: 430157  Cd Length: 117  Bit Score: 56.10  E-value: 7.85e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24656635    17 VYGAVASISAVMYMKWKMEDRVKSAEYYKLALKALRSHRGAVGLLGEPI-------KDSG--------IDLSnannncna 81
Cdd:pfam08695   2 VTFAIFVIGVGAALAYIFNYEKTSSPIYRSTLYQLRRSPEARELLGDNIpfksgwpWISGelntvqgrIDIS-------- 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 24656635    82 eearceVAVRGSKDKGTLYFWASNQPDRG-WLIDRLELET 120
Cdd:pfam08695  74 ------FPVKGSKGSGTVYLTATRESKRHpFHIHEWSLTV 107
 
Name Accession Description Interval E-value
Coa1 pfam08695
Cytochrome oxidase complex assembly protein 1; Coa1 is an inner mitochondrial membrane protein ...
 17-120 7.85e-11

Cytochrome oxidase complex assembly protein 1; Coa1 is an inner mitochondrial membrane protein that associates with Shy1 and is required for cytochrome oxidase complex IV assembly. It contains a conserved hydrophobic segment (amino acids 74-92) with the potential to form a membrane-spanning helix. The N-terminus of Coa1 is rich in positively charged amino acids and could form an amphipathic alpha helix, characteriztic of a mitochondrial presequence. A cleavage site for the mitochondrial processing peptidase is predicted adjacent to the presequence. Upon in vitro import into mitochondria, Coa1 is processed to a mature form, indicating that it possesses a cleavable presequence. The eukaryotic cytochrome oxidase complex consists of 12-13 subunits, with three mitochondrial encoded subunits, Cox1-Cox3, forming the core enzyme. Translation of the Cox1 transcript requires the two promoters, Pet309 and Mss51, and the latter has an additional role in translational elongation. Coa1 is necessary for linking the activity of Mss51 to Cox1 insertion into the assembly complex.


Pssm-ID: 430157  Cd Length: 117  Bit Score: 56.10  E-value: 7.85e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24656635    17 VYGAVASISAVMYMKWKMEDRVKSAEYYKLALKALRSHRGAVGLLGEPI-------KDSG--------IDLSnannncna 81
Cdd:pfam08695   2 VTFAIFVIGVGAALAYIFNYEKTSSPIYRSTLYQLRRSPEARELLGDNIpfksgwpWISGelntvqgrIDIS-------- 73

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 24656635    82 eearceVAVRGSKDKGTLYFWASNQPDRG-WLIDRLELET 120
Cdd:pfam08695  74 ------FPVKGSKGSGTVYLTATRESKRHpFHIHEWSLTV 107
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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