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Conserved domains on  [gi|21358651|ref|NP_647654|]
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phosphatidylinositol glycan anchor biosynthesis class W b, isoform A [Drosophila melanogaster]

Protein Classification

phosphatidylinositol anchor biosynthesis protein PIGW/GWT1( domain architecture ID 5607)

phosphatidylinositol anchor biosynthesis protein PIGW/GWT1 is involved in the addition of the acyl-chain to inositol in an early step of glycosylphosphatidylinositol (GPI) biosynthesis

EC:  2.3.-.-
Gene Ontology:  GO:0016746|GO:0006506

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
GWT1 super family cl02109
GWT1; Glycosylphosphatidylinositol (GPI) is a conserved post-translational modification to ...
85-503 1.10e-35

GWT1; Glycosylphosphatidylinositol (GPI) is a conserved post-translational modification to anchor cell surface proteins to plasma membrane in eukaryotes. GWT1 is involved in GPI anchor biosynthesis; it is required for inositol acylation in yeast.


The actual alignment was detected with superfamily member COG5062:

Pssm-ID: 470470  Cd Length: 429  Bit Score: 137.66  E-value: 1.10e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651  85 YATEFLLVILPCILLVsVAVDYSGYI-GLLMLVATLWFLRTSRALE-----------RARTRVQFDLGGNRPMCLSILRA 152
Cdd:COG5062  41 TLLEFMFCLVPLYSII-VFLKYSGLTyALFLCLLGFLFLFWRRLRKpvnpgnpsskpKQSTDAPQDDEPYTSMAITRYRF 119
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 153 LTHLITAVCILAIDFGSFYRPYRKSRQFGAKLMDTGIGLFVFTMAMVSRRTRhwsdlRRSVIYSALPLILLGLARTVSIL 232
Cdd:COG5062 120 LIIGCTVIAILAVDFPFFPRRLGKSETWGTSLMDIGVGSFVYNSGIVSTRAK-----SKRKLKNALILLFLGFLRYFSVK 194
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 233 TLSYGQDAHEYGQHLNAFFTLGFTkflgSLVSILARKDLHLLpLGFGLLVIHQFGLSVLGISDYVMNEDveRSSFLNANR 312
Cdd:COG5062 195 LLNYQVHVREYGVHWNFFFTLGLL----NLASLFIRTRANFL-LGFFICLTHELLLKFFGLEKFIYSAA--RSSILTSNR 267
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 313 EGLVSLPGFVGLFLLSIYVNRWMVAKSlLTYSEFVRKLRRLFYLVLILWTSFVISAYEigiSRVTCNLGYVIWMLAIGST 392
Cdd:COG5062 268 EGITSLLPYISIFLMGADTGKVVFKKK-PTRKKAWKIIILYNAFFLCVYLVFNFYSTS---SRRLANLPFVMWIMLLHTF 343
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 393 TLCasfGAIDFAINSVmPWDPNPwedhekgllsgeavvkkrggsvvpfTISQALNKNGLTFFLVANILTGMVNIFLRPED 472
Cdd:COG5062 344 HLT---VYELFDRTSK-IYNLVM-------------------------HRFESKNLNFLLVFSNANVLTGKVNSSSKTMS 394
                       410       420       430
                ....*....|....*....|....*....|...
gi 21358651 473 R--SHSESVIILLVYMFLATKLVHELLKRGIRL 503
Cdd:COG5062 395 ScsSHAKCLTYLVAVFSIPCAINSKLAGKLIVS 427
 
Name Accession Description Interval E-value
COG5062 COG5062
Uncharacterized membrane protein [Function unknown];
85-503 1.10e-35

Uncharacterized membrane protein [Function unknown];


Pssm-ID: 227394  Cd Length: 429  Bit Score: 137.66  E-value: 1.10e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651  85 YATEFLLVILPCILLVsVAVDYSGYI-GLLMLVATLWFLRTSRALE-----------RARTRVQFDLGGNRPMCLSILRA 152
Cdd:COG5062  41 TLLEFMFCLVPLYSII-VFLKYSGLTyALFLCLLGFLFLFWRRLRKpvnpgnpsskpKQSTDAPQDDEPYTSMAITRYRF 119
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 153 LTHLITAVCILAIDFGSFYRPYRKSRQFGAKLMDTGIGLFVFTMAMVSRRTRhwsdlRRSVIYSALPLILLGLARTVSIL 232
Cdd:COG5062 120 LIIGCTVIAILAVDFPFFPRRLGKSETWGTSLMDIGVGSFVYNSGIVSTRAK-----SKRKLKNALILLFLGFLRYFSVK 194
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 233 TLSYGQDAHEYGQHLNAFFTLGFTkflgSLVSILARKDLHLLpLGFGLLVIHQFGLSVLGISDYVMNEDveRSSFLNANR 312
Cdd:COG5062 195 LLNYQVHVREYGVHWNFFFTLGLL----NLASLFIRTRANFL-LGFFICLTHELLLKFFGLEKFIYSAA--RSSILTSNR 267
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 313 EGLVSLPGFVGLFLLSIYVNRWMVAKSlLTYSEFVRKLRRLFYLVLILWTSFVISAYEigiSRVTCNLGYVIWMLAIGST 392
Cdd:COG5062 268 EGITSLLPYISIFLMGADTGKVVFKKK-PTRKKAWKIIILYNAFFLCVYLVFNFYSTS---SRRLANLPFVMWIMLLHTF 343
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 393 TLCasfGAIDFAINSVmPWDPNPwedhekgllsgeavvkkrggsvvpfTISQALNKNGLTFFLVANILTGMVNIFLRPED 472
Cdd:COG5062 344 HLT---VYELFDRTSK-IYNLVM-------------------------HRFESKNLNFLLVFSNANVLTGKVNSSSKTMS 394
                       410       420       430
                ....*....|....*....|....*....|...
gi 21358651 473 R--SHSESVIILLVYMFLATKLVHELLKRGIRL 503
Cdd:COG5062 395 ScsSHAKCLTYLVAVFSIPCAINSKLAGKLIVS 427
GWT1 pfam06423
GWT1; Glycosylphosphatidylinositol (GPI) is a conserved post-translational modification to ...
310-465 8.95e-33

GWT1; Glycosylphosphatidylinositol (GPI) is a conserved post-translational modification to anchor cell surface proteins to plasma membrane in eukaryotes. GWT1 is involved in GPI anchor biosynthesis; it is required for inositol acylation in yeast.


Pssm-ID: 461907  Cd Length: 138  Bit Score: 121.55  E-value: 8.95e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651   310 ANREGLVSLPGFVGLFLLSIYVNRWMVAKSLLTYSEFVRK-LRRLFYLVLILWTSFVISAYEIG-ISRVTCNLGYVIWML 387
Cdd:pfam06423   1 ANREGIFSLPGYLAIYLAGVSLGSYVLPRRPTNRVKQWSKlLIKLLILSILFWLLLLVLAFYVEpVSRRLANLPYVLWVV 80
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 21358651   388 AIGSTTLCAsFGAIDFainsvmpwdpnpwedhekgLLSGEAVVKKRGGSVVPFTISQALNKNGLTFFLVANILTGMVN 465
Cdd:pfam06423  81 AFNLTFLLL-YLLVDL-------------------VFFADSAKEKARPSTATPCLLEAINRNGLAVFLLANLLTGLVN 138
 
Name Accession Description Interval E-value
COG5062 COG5062
Uncharacterized membrane protein [Function unknown];
85-503 1.10e-35

Uncharacterized membrane protein [Function unknown];


Pssm-ID: 227394  Cd Length: 429  Bit Score: 137.66  E-value: 1.10e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651  85 YATEFLLVILPCILLVsVAVDYSGYI-GLLMLVATLWFLRTSRALE-----------RARTRVQFDLGGNRPMCLSILRA 152
Cdd:COG5062  41 TLLEFMFCLVPLYSII-VFLKYSGLTyALFLCLLGFLFLFWRRLRKpvnpgnpsskpKQSTDAPQDDEPYTSMAITRYRF 119
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 153 LTHLITAVCILAIDFGSFYRPYRKSRQFGAKLMDTGIGLFVFTMAMVSRRTRhwsdlRRSVIYSALPLILLGLARTVSIL 232
Cdd:COG5062 120 LIIGCTVIAILAVDFPFFPRRLGKSETWGTSLMDIGVGSFVYNSGIVSTRAK-----SKRKLKNALILLFLGFLRYFSVK 194
                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 233 TLSYGQDAHEYGQHLNAFFTLGFTkflgSLVSILARKDLHLLpLGFGLLVIHQFGLSVLGISDYVMNEDveRSSFLNANR 312
Cdd:COG5062 195 LLNYQVHVREYGVHWNFFFTLGLL----NLASLFIRTRANFL-LGFFICLTHELLLKFFGLEKFIYSAA--RSSILTSNR 267
                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 313 EGLVSLPGFVGLFLLSIYVNRWMVAKSlLTYSEFVRKLRRLFYLVLILWTSFVISAYEigiSRVTCNLGYVIWMLAIGST 392
Cdd:COG5062 268 EGITSLLPYISIFLMGADTGKVVFKKK-PTRKKAWKIIILYNAFFLCVYLVFNFYSTS---SRRLANLPFVMWIMLLHTF 343
                       330       340       350       360       370       380       390       400
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651 393 TLCasfGAIDFAINSVmPWDPNPwedhekgllsgeavvkkrggsvvpfTISQALNKNGLTFFLVANILTGMVNIFLRPED 472
Cdd:COG5062 344 HLT---VYELFDRTSK-IYNLVM-------------------------HRFESKNLNFLLVFSNANVLTGKVNSSSKTMS 394
                       410       420       430
                ....*....|....*....|....*....|...
gi 21358651 473 R--SHSESVIILLVYMFLATKLVHELLKRGIRL 503
Cdd:COG5062 395 ScsSHAKCLTYLVAVFSIPCAINSKLAGKLIVS 427
GWT1 pfam06423
GWT1; Glycosylphosphatidylinositol (GPI) is a conserved post-translational modification to ...
310-465 8.95e-33

GWT1; Glycosylphosphatidylinositol (GPI) is a conserved post-translational modification to anchor cell surface proteins to plasma membrane in eukaryotes. GWT1 is involved in GPI anchor biosynthesis; it is required for inositol acylation in yeast.


Pssm-ID: 461907  Cd Length: 138  Bit Score: 121.55  E-value: 8.95e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21358651   310 ANREGLVSLPGFVGLFLLSIYVNRWMVAKSLLTYSEFVRK-LRRLFYLVLILWTSFVISAYEIG-ISRVTCNLGYVIWML 387
Cdd:pfam06423   1 ANREGIFSLPGYLAIYLAGVSLGSYVLPRRPTNRVKQWSKlLIKLLILSILFWLLLLVLAFYVEpVSRRLANLPYVLWVV 80
                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 21358651   388 AIGSTTLCAsFGAIDFainsvmpwdpnpwedhekgLLSGEAVVKKRGGSVVPFTISQALNKNGLTFFLVANILTGMVN 465
Cdd:pfam06423  81 AFNLTFLLL-YLLVDL-------------------VFFADSAKEKARPSTATPCLLEAINRNGLAVFLLANLLTGLVN 138
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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