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Conserved domains on  [gi|21355129|ref|NP_648581|]
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uncharacterized protein Dmel_CG10646 [Drosophila melanogaster]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 204-370 3.80e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 66.20  E-value: 3.80e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 204 DGNLSAYDVRdTQHCAWSINdAHGQMVRDLDCNPNKQChLVTGGDDGYLRIWDCRMPKaPVFERSDHSHWVWCVRFNTFh 283
Cdd:cd00200  72 DKTIRLWDLE-TGECVRTLT-GHTSYVSSVAFSPDGRI-LSSSSRDKTIKVWDVETGK-CLTTLRGHTDWVNSVAFSPD- 146

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 284 DQLILSSSSDCKVLL----------TCAGSVSSETQVQAGLDESNF--SGADAEERHKLLPDG-LLQTFDQHEDSVYCAE 350
Cdd:cd00200 147 GTFVASSSQDGTIKLwdlrtgkcvaTLTGHTGEVNSVAFSPDGEKLlsSSSDGTIKLWDLSTGkCLGTLRGHENGVNSVA 226

                       170       180
                ....*....|....*....|
gi 21355129 351 WSNvDPWIFASLSYDGRVII 370
Cdd:cd00200 227 FSP-DGYLLASGSEDGTIRV 245
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 204-370 3.80e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 66.20  E-value: 3.80e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 204 DGNLSAYDVRdTQHCAWSINdAHGQMVRDLDCNPNKQChLVTGGDDGYLRIWDCRMPKaPVFERSDHSHWVWCVRFNTFh 283
Cdd:cd00200  72 DKTIRLWDLE-TGECVRTLT-GHTSYVSSVAFSPDGRI-LSSSSRDKTIKVWDVETGK-CLTTLRGHTDWVNSVAFSPD- 146

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 284 DQLILSSSSDCKVLL----------TCAGSVSSETQVQAGLDESNF--SGADAEERHKLLPDG-LLQTFDQHEDSVYCAE 350
Cdd:cd00200 147 GTFVASSSQDGTIKLwdlrtgkcvaTLTGHTGEVNSVAFSPDGEKLlsSSSDGTIKLWDLSTGkCLGTLRGHENGVNSVA 226

                       170       180
                ....*....|....*....|
gi 21355129 351 WSNvDPWIFASLSYDGRVII 370
Cdd:cd00200 227 FSP-DGYLLASGSEDGTIRV 245
WD40 COG2319
WD40 repeat [General function prediction only];
204-370 5.56e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 5.56e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 204 DGNLSAYDVrDTQHCAWSINdAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWDCRmPKAPVFERSDHSHWVWCVRFnTFH 283
Cdd:COG2319 225 DGTVRLWDL-ATGKLLRTLT-GHSGSVRSVAFSPDGR-LLASGSADGTVRLWDLA-TGELLRTLTGHSGGVNSVAF-SPD 299

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 284 DQLILSSSSDCKV----------LLTCAGSVSSETQVQagldesnFSgadaeerhkllPDG------------------- 334
Cdd:COG2319 300 GKLLASGSDDGTVrlwdlatgklLRTLTGHTGAVRSVA-------FS-----------PDGktlasgsddgtvrlwdlat 361

                       170       180       190
                ....*....|....*....|....*....|....*...
gi 21355129 335 --LLQTFDQHEDSVYCAEWSNVDPWIfASLSYDGRVII 370
Cdd:COG2319 362 geLLRTLTGHTGAVTSVAFSPDGRTL-ASGSADGTVRL 398
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 224-256 4.91e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 37.29  E-value: 4.91e-04
                           10        20        30
                   ....*....|....*....|....*....|...
gi 21355129    224 DAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWD 256
Cdd:smart00320   9 KGHTGPVTSVAFSPDGK-YLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
224-256 2.25e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 35.40  E-value: 2.25e-03
                          10        20        30
                  ....*....|....*....|....*....|...
gi 21355129   224 DAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWD 256
Cdd:pfam00400   8 EGHTGSVTSLAFSPDGK-LLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 204-370 3.80e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 66.20  E-value: 3.80e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 204 DGNLSAYDVRdTQHCAWSINdAHGQMVRDLDCNPNKQChLVTGGDDGYLRIWDCRMPKaPVFERSDHSHWVWCVRFNTFh 283
Cdd:cd00200  72 DKTIRLWDLE-TGECVRTLT-GHTSYVSSVAFSPDGRI-LSSSSRDKTIKVWDVETGK-CLTTLRGHTDWVNSVAFSPD- 146

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 284 DQLILSSSSDCKVLL----------TCAGSVSSETQVQAGLDESNF--SGADAEERHKLLPDG-LLQTFDQHEDSVYCAE 350
Cdd:cd00200 147 GTFVASSSQDGTIKLwdlrtgkcvaTLTGHTGEVNSVAFSPDGEKLlsSSSDGTIKLWDLSTGkCLGTLRGHENGVNSVA 226

                       170       180
                ....*....|....*....|
gi 21355129 351 WSNvDPWIFASLSYDGRVII 370
Cdd:cd00200 227 FSP-DGYLLASGSEDGTIRV 245
WD40 COG2319
WD40 repeat [General function prediction only];
204-370 5.56e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 5.56e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 204 DGNLSAYDVrDTQHCAWSINdAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWDCRmPKAPVFERSDHSHWVWCVRFnTFH 283
Cdd:COG2319 225 DGTVRLWDL-ATGKLLRTLT-GHSGSVRSVAFSPDGR-LLASGSADGTVRLWDLA-TGELLRTLTGHSGGVNSVAF-SPD 299

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 284 DQLILSSSSDCKV----------LLTCAGSVSSETQVQagldesnFSgadaeerhkllPDG------------------- 334
Cdd:COG2319 300 GKLLASGSDDGTVrlwdlatgklLRTLTGHTGAVRSVA-------FS-----------PDGktlasgsddgtvrlwdlat 361

                       170       180       190
                ....*....|....*....|....*....|....*...
gi 21355129 335 --LLQTFDQHEDSVYCAEWSNVDPWIfASLSYDGRVII 370
Cdd:COG2319 362 geLLRTLTGHTGAVTSVAFSPDGRTL-ASGSADGTVRL 398
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 181-370 1.01e-09

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 58.89  E-value: 1.01e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 181 HTQHFTSGKWSHHHQghqFLT--LQDGNLSAYDVRDTQHCAwSINDaHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWDCR 258
Cdd:cd00200  92 HTSYVSSVAFSPDGR---ILSssSRDKTIKVWDVETGKCLT-TLRG-HTDWVNSVAFSPDGT-FVASSSQDGTIKLWDLR 165

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 259 MPKaPVFERSDHSHWVWCVRFntFHD-QLILSSSSDCKVLL----------TCAGSVSSETQVQAGLDESNFSGADAEER 327
Cdd:cd00200 166 TGK-CVATLTGHTGEVNSVAF--SPDgEKLLSSSSDGTIKLwdlstgkclgTLRGHENGVNSVAFSPDGYLLASGSEDGT 242

                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*.
gi 21355129 328 ---HKLLPDGLLQTFDQHEDSVYCAEWSNVDPWIfASLSYDGRVII 370
Cdd:cd00200 243 irvWDLRTGECVQTLSGHTNSVTSLAWSPDGKRL-ASGSADGTIRI 287
WD40 COG2319
WD40 repeat [General function prediction only];
123-370 1.09e-09

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 59.54  E-value: 1.09e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 123 VLDTEALGERVKTIEFHPNQDTLACVVDNKVAVMQRAESSTRVVAEVPASGSSSGSAKHTQHFTSGKWSHhhQGHQFLTL 202
Cdd:COG2319  61 LLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWDLATGLLLRTLTGHTGAVRSVAFSP--DGKTLASG 138

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 203 -QDGNLSAYDVRDTQhCAWSINdAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWDCRMPKaPVFERSDHSHWVWCVRFNt 281
Cdd:COG2319 139 sADGTVRLWDLATGK-LLRTLT-GHSGAVTSVAFSPDGK-LLASGSDDGTVRLWDLATGK-LLRTLTGHTGAVRSVAFS- 213

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 282 fHD-QLILSSSSDCKVLLTcagSVSSETQVQ--AGLDESNFSGAdaeerhkLLPDG---------------------LLQ 337
Cdd:COG2319 214 -PDgKLLASGSADGTVRLW---DLATGKLLRtlTGHSGSVRSVA-------FSPDGrllasgsadgtvrlwdlatgeLLR 282

                       250       260       270
                ....*....|....*....|....*....|...
gi 21355129 338 TFDQHEDSVYCAEWSNvDPWIFASLSYDGRVII 370
Cdd:COG2319 283 TLTGHSGGVNSVAFSP-DGKLLASGSDDGTVRL 314
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 226-370 3.50e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 54.26  E-value: 3.50e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 226 HGQMVRDLDCNPNKQcHLVTGGDDGYLRIWDCRmPKAPVFERSDHSHWVWCVRFnTFHDQLILSSSSD--CKVlltcags 303
Cdd:cd00200   8 HTGGVTCVAFSPDGK-LLATGSGDGTIKVWDLE-TGELLRTLKGHTGPVRDVAA-SADGTYLASGSSDktIRL------- 77

                        90       100       110       120       130       140
                ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 21355129 304 vssetqvqagLDESNfsgadaeerhkllpDGLLQTFDQHEDSVYCAEWSNVDPWIFASlSYDGRVII 370
Cdd:cd00200  78 ----------WDLET--------------GECVRTLTGHTSYVSSVAFSPDGRILSSS-SRDKTIKV 119
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 226-370 3.98e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 54.26  E-value: 3.98e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 226 HGQMVRDLDCNPNKQChLVTGGDDGYLRIWDCRMPKaPVFERSDHSHWVWCVRFNTfHDQLILSSSSDCKVLL------- 298
Cdd:cd00200  50 HTGPVRDVAASADGTY-LASGSSDKTIRLWDLETGE-CVRTLTGHTSYVSSVAFSP-DGRILSSSSRDKTIKVwdvetgk 126

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21355129 299 ---TCAG--------SVSSETQVQAGldesnfSGADAEERhklLPDG----LLQTFDQHEDSVYCAEWSNVDPWIFASlS 363
Cdd:cd00200 127 cltTLRGhtdwvnsvAFSPDGTFVAS------SSQDGTIK---LWDLrtgkCVATLTGHTGEVNSVAFSPDGEKLLSS-S 196


                ....*..
gi 21355129 364 YDGRVII 370
Cdd:cd00200 197 SDGTIKL 203
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 224-256 4.91e-04

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 37.29  E-value: 4.91e-04
                           10        20        30
                   ....*....|....*....|....*....|...
gi 21355129    224 DAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWD 256
Cdd:smart00320   9 KGHTGPVTSVAFSPDGK-YLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
224-256 2.25e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 35.40  E-value: 2.25e-03
                          10        20        30
                  ....*....|....*....|....*....|...
gi 21355129   224 DAHGQMVRDLDCNPNKQcHLVTGGDDGYLRIWD 256
Cdd:pfam00400   8 EGHTGSVTSLAFSPDGK-LLASGSDDGTVKVWD 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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