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Conserved domains on  [gi|24663500|ref|NP_648603|]
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splicing factor 3a subunit 2 [Drosophila melanogaster]

Protein Classification

PRP11 family protein( domain architecture ID 711366)

PRP11 family protein similar to Dictyostelium discoideum splicing factor 3A subunit 2 which is involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex

CATH:  2.60.40.2690
Gene Ontology:  GO:0000398|GO:0008270|GO:0003676|GO:0000245

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PRP11 super family cl25587
Splicing factor 3a, subunit 2 [RNA processing and modification];
1-214 1.85e-78

Splicing factor 3a, subunit 2 [RNA processing and modification];


The actual alignment was detected with superfamily member COG5246:

Pssm-ID: 227571 [Multi-domain]  Cd Length: 222  Bit Score: 236.49  E-value: 1.85e-78
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500   1 MDFQNRAGGKTGSGGVASWSETNRDRKERLRQLALETIDLNKDPYFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQD 80
Cdd:COG5246   1 MNYGGRGGSKTGGGGTASEIKKNKKRRSRQLLLEAYGQDLMDDPYLSKNHTGKYVCLLCKTKHLTEMSYVKHREGKKHKE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500  81 NLARRAAKEAKE----APSSLLAPEKPRVEPKK-FVKIGRPGYRVTKQRELSNGQQSLLFQVDYPEITESIVPRHRFMSA 155
Cdd:COG5246  81 NSSRRSEEKSSLgrenQTTHDFRQQQKIIEAKQsLKRSGIPSYKVRSLVEPKGGRRGLGIQVNYEDDLAEEMPQYRIVSS 160

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 24663500 156 YEQKIEPPDRKWQYLLFAAEPYETIGFKVPsreveKSEGKFW---THWNRDTKQFFLQFAFK 214
Cdd:COG5246 161 LEQNVEEYDESFRYLVFACEPYENIAFKFE-----NKEIDFLsiyEDWDAETGTYTLQFFFL 217
 
Name Accession Description Interval E-value
PRP11 COG5246
Splicing factor 3a, subunit 2 [RNA processing and modification];
1-214 1.85e-78

Splicing factor 3a, subunit 2 [RNA processing and modification];


Pssm-ID: 227571 [Multi-domain]  Cd Length: 222  Bit Score: 236.49  E-value: 1.85e-78
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500   1 MDFQNRAGGKTGSGGVASWSETNRDRKERLRQLALETIDLNKDPYFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQD 80
Cdd:COG5246   1 MNYGGRGGSKTGGGGTASEIKKNKKRRSRQLLLEAYGQDLMDDPYLSKNHTGKYVCLLCKTKHLTEMSYVKHREGKKHKE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500  81 NLARRAAKEAKE----APSSLLAPEKPRVEPKK-FVKIGRPGYRVTKQRELSNGQQSLLFQVDYPEITESIVPRHRFMSA 155
Cdd:COG5246  81 NSSRRSEEKSSLgrenQTTHDFRQQQKIIEAKQsLKRSGIPSYKVRSLVEPKGGRRGLGIQVNYEDDLAEEMPQYRIVSS 160

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 24663500 156 YEQKIEPPDRKWQYLLFAAEPYETIGFKVPsreveKSEGKFW---THWNRDTKQFFLQFAFK 214
Cdd:COG5246 161 LEQNVEEYDESFRYLVFACEPYENIAFKFE-----NKEIDFLsiyEDWDAETGTYTLQFFFL 217
SF3A2 pfam16835
Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11); SF3A2 is one of the components of the ...
 114-207 1.19e-58

Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11); SF3A2 is one of the components of the SF3a splicing factor complex of the mature U2 snRNP (small nuclear ribonucleoprotein particle). In yeast, SF3a shows a bifurcated assembly structure of three subunits, Prp9 (subunit 3), Prp11 (subunit 2) and Prp21 (subunit 1). with Prp21 wrapping around Prp11.


Pssm-ID: 435601  Cd Length: 95  Bit Score: 181.58  E-value: 1.19e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500   114 GRPGYRVTKQRELSNGQQSLLFQVDYPEITESIVPRHRFMSAYEQKIE-PPDRKWQYLLFAAEPYETIGFKVPSREVEKS 192
Cdd:pfam16835   1 GRPGYKVTKVRDPETGQLGLLFQVDYPEIAEGVKPRHRIMSAYEQKVEtPPDKNYQYLVIAAEPYETIAFKIPNREIDRL 80

                          90
                  ....*....|....*
gi 24663500   193 EGKFWTHWNRDTKQF 207
Cdd:pfam16835  81 EGKFWTYWDPDTKEF 95
CactinC_cactus smart01050
Cactus-binding C-terminus of cactin protein; CactinC_cactus is the C-terminal 200 residues of ...
 100-214 5.11e-35

Cactus-binding C-terminus of cactin protein; CactinC_cactus is the C-terminal 200 residues of the cactin protein which are necessary for the association of cactin with IkappaB-cactus as one of the intracellular members of the Rel complex. The Rel (NF-kappaB) pathway is conserved in invertebrates and vertebrates. In mammals, it controls the activities of the immune and inflammatory response genes as well as viral genes, and is critical for cell growth and survival. In Drosophila, the Rel pathway functions in the innate cellular and humoral immune response, in muscle development, and in the establishment of dorsal-ventral polarity in the early embryo. Most members of the family also have a Cactin_mid domain further upstream.


Pssm-ID: 214996  Cd Length: 129  Bit Score: 122.08  E-value: 5.11e-35
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500    100 PEKPRVEPKKFVKIGRPGYRVTK---QRELSNGQQSLLFQVDYPEIT-ESIVPRHRFMSAYEQkiEPPDRKWQYLLF-AA 174
Cdd:smart01050   7 LRKPRYFNRVKTGYDWNKYNQTHydnDNPPPKIVQGYKFNIFYPDLIdKGKAPRYRIESSFEI--EKPDDDTCLLRFsAG 84

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 24663500    175 EPYETIGFKVPSREVEKSE-GKFWTHWNRdtKQFFLQFAFK 214
Cdd:smart01050  85 PPYEDIAFKIVNREWDYSHrGGFKSSFDN--GILQLQFNFK 123
 
Name Accession Description Interval E-value
PRP11 COG5246
Splicing factor 3a, subunit 2 [RNA processing and modification];
1-214 1.85e-78

Splicing factor 3a, subunit 2 [RNA processing and modification];


Pssm-ID: 227571 [Multi-domain]  Cd Length: 222  Bit Score: 236.49  E-value: 1.85e-78
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500   1 MDFQNRAGGKTGSGGVASWSETNRDRKERLRQLALETIDLNKDPYFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQD 80
Cdd:COG5246   1 MNYGGRGGSKTGGGGTASEIKKNKKRRSRQLLLEAYGQDLMDDPYLSKNHTGKYVCLLCKTKHLTEMSYVKHREGKKHKE 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500  81 NLARRAAKEAKE----APSSLLAPEKPRVEPKK-FVKIGRPGYRVTKQRELSNGQQSLLFQVDYPEITESIVPRHRFMSA 155
Cdd:COG5246  81 NSSRRSEEKSSLgrenQTTHDFRQQQKIIEAKQsLKRSGIPSYKVRSLVEPKGGRRGLGIQVNYEDDLAEEMPQYRIVSS 160

                       170       180       190       200       210       220
                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 24663500 156 YEQKIEPPDRKWQYLLFAAEPYETIGFKVPsreveKSEGKFW---THWNRDTKQFFLQFAFK 214
Cdd:COG5246 161 LEQNVEEYDESFRYLVFACEPYENIAFKFE-----NKEIDFLsiyEDWDAETGTYTLQFFFL 217
SF3A2 pfam16835
Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11); SF3A2 is one of the components of the ...
 114-207 1.19e-58

Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11); SF3A2 is one of the components of the SF3a splicing factor complex of the mature U2 snRNP (small nuclear ribonucleoprotein particle). In yeast, SF3a shows a bifurcated assembly structure of three subunits, Prp9 (subunit 3), Prp11 (subunit 2) and Prp21 (subunit 1). with Prp21 wrapping around Prp11.


Pssm-ID: 435601  Cd Length: 95  Bit Score: 181.58  E-value: 1.19e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500   114 GRPGYRVTKQRELSNGQQSLLFQVDYPEITESIVPRHRFMSAYEQKIE-PPDRKWQYLLFAAEPYETIGFKVPSREVEKS 192
Cdd:pfam16835   1 GRPGYKVTKVRDPETGQLGLLFQVDYPEIAEGVKPRHRIMSAYEQKVEtPPDKNYQYLVIAAEPYETIAFKIPNREIDRL 80

                          90
                  ....*....|....*
gi 24663500   193 EGKFWTHWNRDTKQF 207
Cdd:pfam16835  81 EGKFWTYWDPDTKEF 95
CactinC_cactus smart01050
Cactus-binding C-terminus of cactin protein; CactinC_cactus is the C-terminal 200 residues of ...
 100-214 5.11e-35

Cactus-binding C-terminus of cactin protein; CactinC_cactus is the C-terminal 200 residues of the cactin protein which are necessary for the association of cactin with IkappaB-cactus as one of the intracellular members of the Rel complex. The Rel (NF-kappaB) pathway is conserved in invertebrates and vertebrates. In mammals, it controls the activities of the immune and inflammatory response genes as well as viral genes, and is critical for cell growth and survival. In Drosophila, the Rel pathway functions in the innate cellular and humoral immune response, in muscle development, and in the establishment of dorsal-ventral polarity in the early embryo. Most members of the family also have a Cactin_mid domain further upstream.


Pssm-ID: 214996  Cd Length: 129  Bit Score: 122.08  E-value: 5.11e-35
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24663500    100 PEKPRVEPKKFVKIGRPGYRVTK---QRELSNGQQSLLFQVDYPEIT-ESIVPRHRFMSAYEQkiEPPDRKWQYLLF-AA 174
Cdd:smart01050   7 LRKPRYFNRVKTGYDWNKYNQTHydnDNPPPKIVQGYKFNIFYPDLIdKGKAPRYRIESSFEI--EKPDDDTCLLRFsAG 84

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 24663500    175 EPYETIGFKVPSREVEKSE-GKFWTHWNRdtKQFFLQFAFK 214
Cdd:smart01050  85 PPYEDIAFKIVNREWDYSHrGGFKSSFDN--GILQLQFNFK 123
ZnF_U1 smart00451
U1-like zinc finger; Family of C2H2-type zinc fingers, present in matrin, U1 small nuclear ...
 52-85 4.12e-08

U1-like zinc finger; Family of C2H2-type zinc fingers, present in matrin, U1 small nuclear ribonucleoprotein C and other RNA-binding proteins.


Pssm-ID: 197732 [Multi-domain]  Cd Length: 35  Bit Score: 48.02  E-value: 4.12e-08
                           10        20        30
                   ....*....|....*....|....*....|....
gi 24663500     52 GSYECKLCLTLHNNEGSYLAHTQGKKHQDNLARR 85
Cdd:smart00451   2 GGFYCKLCNVTFTDEISVEAHLKGKKHKKNVKKR 35
zf-met pfam12874
Zinc-finger of C2H2 type; This is a zinc-finger domain with the CxxCx(12)Hx(6)H motif, found ...
 54-78 2.72e-06

Zinc-finger of C2H2 type; This is a zinc-finger domain with the CxxCx(12)Hx(6)H motif, found in multiple copies in a wide range of proteins from plants to metazoans. Some member proteins, particularly those from plants, are annotated as being RNA-binding.


Pssm-ID: 463736 [Multi-domain]  Cd Length: 25  Bit Score: 42.87  E-value: 2.72e-06
                          10        20
                  ....*....|....*....|....*
gi 24663500    54 YECKLCLTLHNNEGSYLAHTQGKKH 78
Cdd:pfam12874   1 FYCELCNVTFNSESQLKSHLQGKKH 25
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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