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Conserved domains on  [gi|24650003|ref|NP_651373|]
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uncharacterized protein Dmel_CG31300 [Drosophila melanogaster]

Protein Classification

protein kinase family protein( domain architecture ID 10503049)

protein kinase family protein, similar to Bombyx mori EcKinase that catalyzes the phosphorylation of the steroid hormone and together with ecdysteroid-phosphate phosphatase, plays an important role in ecdysteroid economy of the ovary-egg system

CATH:  1.10.510.10
EC:  2.7.-.-
Gene Ontology:  GO:0016310|GO:0005524|GO:0016301
PubMed:  16244704
SCOP:  4003661

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 52-341 2.88e-112

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


:

Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 331.16  E-value: 2.88e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    52 QGDHYASVMFRTTAEC-TTAKGKFSRPLIIKAMPeQDGHKKDMLSESHLFETEIGMYCQVLPEFERILRESGDDTKLFVP 130
Cdd:pfam02958   1 KGDNYASVMLRVTIEYeKGDGSKRTKSLIVKTMP-DNEERREMFSSLNLFTREINMYEKVLPELEALYREAGDPFKLAPK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   131 CIYHSLEPRK-VMIFEDLVPQGYYVI-RDRPVAQEELKTAFAKLAKWHAISMKYIKEQPDFLKEFKYGLFEMPTVK---- 204
Cdd:pfam02958  80 CYYADLEPEDqVIILEDLSLKGYKNAdRLKGLDLEHTKLVLEKLAKFHAASAALKELQPEVFKQLKKGLFEEDYVNgaik 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   205 --TDPFITTGMQSFIEML-DRLPELRKYKPHFEKIKDKYMQRLQAVMKeyhenRKSDAFYVLCHGDFHLRNMMFKNNKgT 281
Cdd:pfam02958 160 efFEPLMETGLDAAAEALrEQLPEYEKYAEKLEKLKDNYFDRLLRLVE-----PTPGEFNVLNHGDLWVNNIMFKYDD-E 233

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   282 GAHEDTMLVDFQISNLCPITIDLTYSIYMLMEPEQRREMGKDLINHYLTVLVATLKSIGY 341
Cdd:pfam02958 234 GEPEDVILVDFQLSRYGSPAIDLNYFLYTSTELELRLEHFDELLRYYHSSLVETLKKLGY 293
 
Name Accession Description Interval E-value
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 52-341 2.88e-112

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 331.16  E-value: 2.88e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    52 QGDHYASVMFRTTAEC-TTAKGKFSRPLIIKAMPeQDGHKKDMLSESHLFETEIGMYCQVLPEFERILRESGDDTKLFVP 130
Cdd:pfam02958   1 KGDNYASVMLRVTIEYeKGDGSKRTKSLIVKTMP-DNEERREMFSSLNLFTREINMYEKVLPELEALYREAGDPFKLAPK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   131 CIYHSLEPRK-VMIFEDLVPQGYYVI-RDRPVAQEELKTAFAKLAKWHAISMKYIKEQPDFLKEFKYGLFEMPTVK---- 204
Cdd:pfam02958  80 CYYADLEPEDqVIILEDLSLKGYKNAdRLKGLDLEHTKLVLEKLAKFHAASAALKELQPEVFKQLKKGLFEEDYVNgaik 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   205 --TDPFITTGMQSFIEML-DRLPELRKYKPHFEKIKDKYMQRLQAVMKeyhenRKSDAFYVLCHGDFHLRNMMFKNNKgT 281
Cdd:pfam02958 160 efFEPLMETGLDAAAEALrEQLPEYEKYAEKLEKLKDNYFDRLLRLVE-----PTPGEFNVLNHGDLWVNNIMFKYDD-E 233

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   282 GAHEDTMLVDFQISNLCPITIDLTYSIYMLMEPEQRREMGKDLINHYLTVLVATLKSIGY 341
Cdd:pfam02958 234 GEPEDVILVDFQLSRYGSPAIDLNYFLYTSTELELRLEHFDELLRYYHSSLVETLKKLGY 293
CHK smart00587
ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases
 142-338 1.74e-58

ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases


Pssm-ID: 214734  Cd Length: 196  Bit Score: 189.85  E-value: 1.74e-58
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    142 MIFEDLVPQGYYVI-RDRPVAQEELKTAFAKLAKWHAISMKYI-KEQPDFLKEFKYGLFEMPTVKTDPFITTGMQSFIEM 219
Cdd:smart00587   1 IIFEDLSPKGYVNAdRLKGLDLEHTSLVLKKLAKLHAASAVLIeEEKGSYLEEFDEGLFERFKRMFSEEFIGGLENFLRE 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    220 LDRLPELRKyKPHFEKIKDKYMQRLQAVMKEYHENRKSDaFYVLCHGDFHLRNMMFKNNKgTGAHEDTMLVDFQISNLCP 299
Cdd:smart00587  81 LLSQPELLK-VEEYIEKLDKLLDNLEDLKKEDKEPDEGE-FNVLNHGDLWANNIMFKYDD-EGKPEDVALIDFQLSHYGS 157

                          170       180       190
                   ....*....|....*....|....*....|....*....
gi 24650003    300 ITIDLTYSIYMLMEPEQRREMGKDLINHYLTVLVATLKS 338
Cdd:smart00587 158 PAEDLHYFLLTSLSVEIRREHFDELLKFYYETLVETLKK 196
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 213-328 9.03e-05

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 43.95  E-value: 9.03e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003 213 MQSFIEMLDRLPELRkykphfekikDKYMQRLQAVMKEYHENrksdafyVLCHGDFHLRNMMFKNNKGtgahEDTMLVDF 292
Cdd:COG3173 161 LRRALARTDDLPALR----------ERLAAWLAANLPEWGPP-------VLVHGDLRPGNLLVDPDDG----RLTAVIDW 219

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 24650003 293 QISNLCPITIDLTYSIYMLMEPEQRREMGKDLINHY 328
Cdd:COG3173 220 ELATLGDPAADLAYLLLYWRLPDDLLGPRAAFLAAY 255
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 261-306 2.88e-03

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 38.05  E-value: 2.88e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*.
gi 24650003 261 YVLCHGDFHLRNMMFKNNKgtgahEDTMLVDFQISNLCPITIDLTY 306
Cdd:cd05120 111 SVLTHGDLHPGNILVKPDG-----KLSGIIDWEFAGYGPPAFDYAA 151
 
Name Accession Description Interval E-value
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 52-341 2.88e-112

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 331.16  E-value: 2.88e-112
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    52 QGDHYASVMFRTTAEC-TTAKGKFSRPLIIKAMPeQDGHKKDMLSESHLFETEIGMYCQVLPEFERILRESGDDTKLFVP 130
Cdd:pfam02958   1 KGDNYASVMLRVTIEYeKGDGSKRTKSLIVKTMP-DNEERREMFSSLNLFTREINMYEKVLPELEALYREAGDPFKLAPK 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   131 CIYHSLEPRK-VMIFEDLVPQGYYVI-RDRPVAQEELKTAFAKLAKWHAISMKYIKEQPDFLKEFKYGLFEMPTVK---- 204
Cdd:pfam02958  80 CYYADLEPEDqVIILEDLSLKGYKNAdRLKGLDLEHTKLVLEKLAKFHAASAALKELQPEVFKQLKKGLFEEDYVNgaik 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   205 --TDPFITTGMQSFIEML-DRLPELRKYKPHFEKIKDKYMQRLQAVMKeyhenRKSDAFYVLCHGDFHLRNMMFKNNKgT 281
Cdd:pfam02958 160 efFEPLMETGLDAAAEALrEQLPEYEKYAEKLEKLKDNYFDRLLRLVE-----PTPGEFNVLNHGDLWVNNIMFKYDD-E 233

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   282 GAHEDTMLVDFQISNLCPITIDLTYSIYMLMEPEQRREMGKDLINHYLTVLVATLKSIGY 341
Cdd:pfam02958 234 GEPEDVILVDFQLSRYGSPAIDLNYFLYTSTELELRLEHFDELLRYYHSSLVETLKKLGY 293
CHK smart00587
ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases
 142-338 1.74e-58

ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases


Pssm-ID: 214734  Cd Length: 196  Bit Score: 189.85  E-value: 1.74e-58
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    142 MIFEDLVPQGYYVI-RDRPVAQEELKTAFAKLAKWHAISMKYI-KEQPDFLKEFKYGLFEMPTVKTDPFITTGMQSFIEM 219
Cdd:smart00587   1 IIFEDLSPKGYVNAdRLKGLDLEHTSLVLKKLAKLHAASAVLIeEEKGSYLEEFDEGLFERFKRMFSEEFIGGLENFLRE 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003    220 LDRLPELRKyKPHFEKIKDKYMQRLQAVMKEYHENRKSDaFYVLCHGDFHLRNMMFKNNKgTGAHEDTMLVDFQISNLCP 299
Cdd:smart00587  81 LLSQPELLK-VEEYIEKLDKLLDNLEDLKKEDKEPDEGE-FNVLNHGDLWANNIMFKYDD-EGKPEDVALIDFQLSHYGS 157

                          170       180       190
                   ....*....|....*....|....*....|....*....
gi 24650003    300 ITIDLTYSIYMLMEPEQRREMGKDLINHYLTVLVATLKS 338
Cdd:smart00587 158 PAEDLHYFLLTSLSVEIRREHFDELLKFYYETLVETLKK 196
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 109-318 2.31e-08

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 54.43  E-value: 2.31e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   109 QVLPEFE--RILRESGDDT--KLFVPCIYHSLEPRKVMIFEDLVPQGYYVIRDRPVAQEELKTAFAKLAKWHAIsmkyik 184
Cdd:pfam01636  36 ELRRELAllRHLAAAGVPPvpRVLAGCTDAELLGLPFLLMEYLPGEVLARPLLPEERGALLEALGRALARLHAV------ 109

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003   185 eQPDFLKEFKyglFEMPTVKTDPFITTgmqsfIEMLDRLPELRKYKPhfekikdkymQRLQAVMKEYHENRKSDAFYVLC 264
Cdd:pfam01636 110 -DPAALPLAG---RLARLLELLRQLEA-----ALARLLAAELLDRLE----------ELEERLLAALLALLPAELPPVLV 170

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 24650003   265 HGDFHLRNMMF-KNNKGTGahedtmLVDFQISNLCPITIDLTYSIYMLMEPEQRR 318
Cdd:pfam01636 171 HGDLHPGNLLVdPGGRVSG------VIDFEDAGLGDPAYDLAILLNSWGRELGAE 219
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 213-328 9.03e-05

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 43.95  E-value: 9.03e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003 213 MQSFIEMLDRLPELRkykphfekikDKYMQRLQAVMKEYHENrksdafyVLCHGDFHLRNMMFKNNKGtgahEDTMLVDF 292
Cdd:COG3173 161 LRRALARTDDLPALR----------ERLAAWLAANLPEWGPP-------VLVHGDLRPGNLLVDPDDG----RLTAVIDW 219

                        90       100       110
                ....*....|....*....|....*....|....*.
gi 24650003 293 QISNLCPITIDLTYSIYMLMEPEQRREMGKDLINHY 328
Cdd:COG3173 220 ELATLGDPAADLAYLLLYWRLPDDLLGPRAAFLAAY 255
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
220-320 1.10e-04

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 42.46  E-value: 1.10e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650003 220 LDRLPELRKYKPHFEKIKDKYMQRLQAVMKEYhenRKSDAFYVLCHGDFHLRNMMFKNNKgtgaheDTMLVDFQISNLCP 299
Cdd:COG0510  11 FDLFARLERYLALGPRDLPELLRRLEELERAL---AARPLPLVLCHGDLHPGNFLVTDDG------RLYLIDWEYAGLGD 81

                        90       100
                ....*....|....*....|..
gi 24650003 300 ITIDL-TYSIYMLMEPEQRREM 320
Cdd:COG0510  82 PAFDLaALLVEYGLSPEQAEEL 103
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 261-306 2.88e-03

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 38.05  E-value: 2.88e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*.
gi 24650003 261 YVLCHGDFHLRNMMFKNNKgtgahEDTMLVDFQISNLCPITIDLTY 306
Cdd:cd05120 111 SVLTHGDLHPGNILVKPDG-----KLSGIIDWEFAGYGPPAFDYAA 151
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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