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Conserved domains on  [gi|665395316|ref|NP_651656|]
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strictosidine synthase-like 2 [Drosophila melanogaster]

Protein Classification

SMP-30/gluconolactonase/LRE family protein( domain architecture ID 11465288)

SMP-30/gluconolactonase/LRE family protein similar to Staphylococcus lactonase drp35 and Zymomonas mobilis gluconolactonase, which hydrolyzes the gluconolactone formed by glucose-fructose oxidoreductase to form gluconate;

CATH:  2.120.10.30
EC:  3.1.1.-
Gene Ontology:  GO:0005515|GO:0046872|GO:0016787
PubMed:  9868369
SCOP:  4003251

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
YvrE COG3386
Sugar lactone lactonase YvrE [Carbohydrate transport and metabolism]; Sugar lactone lactonase ...
 61-284 3.08e-28

Sugar lactone lactonase YvrE [Carbohydrate transport and metabolism]; Sugar lactone lactonase YvrE is part of the Pathway/BioSystem: Non-phosphorylated Entner-Doudoroff pathway


:

Pssm-ID: 442613 [Multi-domain]  Cd Length: 266  Bit Score: 111.91  E-value: 3.08e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  61 KLWKDQIFGPECLIVLEDK--IYTGIHSGEVIRLNNEEsvqpitkiGQPCDYIFDDelcGYPVGLALDTQGNnLIVSDAY 138
Cdd:COG3386    1 KLADAGFRLGEGPVWDPDGrlYWVDIPGGRIHRYDPDG--------GAVEVFAEPS---GRPNGLAFDPDGR-LLVADHG 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316 139 YGIWQVDLETKKKTVLVPAeqilPGKGANRraklFNSLVISRQGDIFWTDSFSEDfvfaafanPSGRLFRYDRVKKTnEV 218
Cdd:COG3386   69 RGLVRFDPADGEVTVLADE----YGKPLNR----PNDGVVDPDGRLYFTDMGEYL--------PTGALYRVDPDGSL-RV 131

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316 219 LLDELSFANGLALSPSEDFIILAETTAMRLRRYYLKGSRA-GESEVFVE--GLPGCPDNLTADEEG-IWV 284
Cdd:COG3386  132 LADGLTFPNGIAFSPDGRTLYVADTGAGRIYRFDLDADGTlGNRRVFADlpDGPGGPDGLAVDADGnLWV 201
 
Name Accession Description Interval E-value
YvrE COG3386
Sugar lactone lactonase YvrE [Carbohydrate transport and metabolism]; Sugar lactone lactonase ...
 61-284 3.08e-28

Sugar lactone lactonase YvrE [Carbohydrate transport and metabolism]; Sugar lactone lactonase YvrE is part of the Pathway/BioSystem: Non-phosphorylated Entner-Doudoroff pathway


Pssm-ID: 442613 [Multi-domain]  Cd Length: 266  Bit Score: 111.91  E-value: 3.08e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  61 KLWKDQIFGPECLIVLEDK--IYTGIHSGEVIRLNNEEsvqpitkiGQPCDYIFDDelcGYPVGLALDTQGNnLIVSDAY 138
Cdd:COG3386    1 KLADAGFRLGEGPVWDPDGrlYWVDIPGGRIHRYDPDG--------GAVEVFAEPS---GRPNGLAFDPDGR-LLVADHG 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316 139 YGIWQVDLETKKKTVLVPAeqilPGKGANRraklFNSLVISRQGDIFWTDSFSEDfvfaafanPSGRLFRYDRVKKTnEV 218
Cdd:COG3386   69 RGLVRFDPADGEVTVLADE----YGKPLNR----PNDGVVDPDGRLYFTDMGEYL--------PTGALYRVDPDGSL-RV 131

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316 219 LLDELSFANGLALSPSEDFIILAETTAMRLRRYYLKGSRA-GESEVFVE--GLPGCPDNLTADEEG-IWV 284
Cdd:COG3386  132 LADGLTFPNGIAFSPDGRTLYVADTGAGRIYRFDLDADGTlGNRRVFADlpDGPGGPDGLAVDADGnLWV 201
Str_synth pfam03088
Strictosidine synthase; Strictosidine synthase (E.C. 4.3.3.2) is a key enzyme in alkaloid ...
 174-256 9.01e-18

Strictosidine synthase; Strictosidine synthase (E.C. 4.3.3.2) is a key enzyme in alkaloid biosynthesis. It catalyzes the condensation of tryptamine with secologanin to form strictosidine.


Pssm-ID: 281131 [Multi-domain]  Cd Length: 89  Bit Score: 77.87  E-value: 9.01e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  174 NSL-VISRQGDIFWTDSFSE-DF--VFAAF--ANPSGRLFRYDRVKKTNEVLLDELSFANGLALSPSEDFIILAETTAMR 247
Cdd:pfam03088   1 NALdVDPETGVLYFTDSSSRyDRrqVIAAFleGDATGRLMKYDPTTKVTKVLLDDLYFPNGIALSPDGSFVLFCETPMAR 80


                  ....*....
gi 665395316  248 LRRYYLKGS 256
Cdd:pfam03088  81 ISRYWIKEP 89
 
Name Accession Description Interval E-value
YvrE COG3386
Sugar lactone lactonase YvrE [Carbohydrate transport and metabolism]; Sugar lactone lactonase ...
 61-284 3.08e-28

Sugar lactone lactonase YvrE [Carbohydrate transport and metabolism]; Sugar lactone lactonase YvrE is part of the Pathway/BioSystem: Non-phosphorylated Entner-Doudoroff pathway


Pssm-ID: 442613 [Multi-domain]  Cd Length: 266  Bit Score: 111.91  E-value: 3.08e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  61 KLWKDQIFGPECLIVLEDK--IYTGIHSGEVIRLNNEEsvqpitkiGQPCDYIFDDelcGYPVGLALDTQGNnLIVSDAY 138
Cdd:COG3386    1 KLADAGFRLGEGPVWDPDGrlYWVDIPGGRIHRYDPDG--------GAVEVFAEPS---GRPNGLAFDPDGR-LLVADHG 68

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316 139 YGIWQVDLETKKKTVLVPAeqilPGKGANRraklFNSLVISRQGDIFWTDSFSEDfvfaafanPSGRLFRYDRVKKTnEV 218
Cdd:COG3386   69 RGLVRFDPADGEVTVLADE----YGKPLNR----PNDGVVDPDGRLYFTDMGEYL--------PTGALYRVDPDGSL-RV 131

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316 219 LLDELSFANGLALSPSEDFIILAETTAMRLRRYYLKGSRA-GESEVFVE--GLPGCPDNLTADEEG-IWV 284
Cdd:COG3386  132 LADGLTFPNGIAFSPDGRTLYVADTGAGRIYRFDLDADGTlGNRRVFADlpDGPGGPDGLAVDADGnLWV 201
Str_synth pfam03088
Strictosidine synthase; Strictosidine synthase (E.C. 4.3.3.2) is a key enzyme in alkaloid ...
 174-256 9.01e-18

Strictosidine synthase; Strictosidine synthase (E.C. 4.3.3.2) is a key enzyme in alkaloid biosynthesis. It catalyzes the condensation of tryptamine with secologanin to form strictosidine.


Pssm-ID: 281131 [Multi-domain]  Cd Length: 89  Bit Score: 77.87  E-value: 9.01e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  174 NSL-VISRQGDIFWTDSFSE-DF--VFAAF--ANPSGRLFRYDRVKKTNEVLLDELSFANGLALSPSEDFIILAETTAMR 247
Cdd:pfam03088   1 NALdVDPETGVLYFTDSSSRyDRrqVIAAFleGDATGRLMKYDPTTKVTKVLLDDLYFPNGIALSPDGSFVLFCETPMAR 80


                  ....*....
gi 665395316  248 LRRYYLKGS 256
Cdd:pfam03088  81 ISRYWIKEP 89
SGL pfam08450
SMP-30/Gluconolactonase/LRE-like region; This family describes a region that is found in ...
 120-284 5.81e-15

SMP-30/Gluconolactonase/LRE-like region; This family describes a region that is found in proteins expressed by a variety of eukaryotic and prokaryotic species. These proteins include various enzymes, such as senescence marker protein 30 (SMP-30), gluconolactonase and luciferin-regenerating enzyme (LRE). SMP-30 is known to hydrolyse diisopropyl phosphorofluoridate in the liver, and has been noted as having sequence similarity, in the region described in this family, with PON1 and LRE.


Pssm-ID: 462480 [Multi-domain]  Cd Length: 246  Bit Score: 74.22  E-value: 5.81e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  120 PVGLALDTQGNNLIVSDAYyGIWQVDLETKKKTVLVpaeQILPGKGANRRaklFNSLVISRQGDiFWTDSFSEDFvfaAF 199
Cdd:pfam08450  42 PVGAIAPRDDGGLIVALKD-GVALLDLATGELTPLA---DPEDDDWPLNR---FNDGKVDPDGR-FWFGTMGDDE---AP 110

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 665395316  200 ANPSGRLFRYDRVKKTnEVLLDELSFANGLALSPSEDFIILAETTAMRLRRY-Y-LKGSRAGESEVFV--EGLPGCPDNL 275
Cdd:pfam08450 111 GGDPGALYRLDPDGKL-TRVLDGLTISNGLAWSPDGRTLYFADSPARKIWAYdYdLDGGLISNRRVFAdfKPGLGRPDGM 189

                         170
                  ....*....|
gi 665395316  276 TADEEG-IWV 284
Cdd:pfam08450 190 AVDAEGnVWV 199
SSL_N pfam20067
Strictosidine synthase-like, N-terminal; This domain is found at the N-terminal of ...
 53-92 4.51e-04

Strictosidine synthase-like, N-terminal; This domain is found at the N-terminal of strictosidine synthase-like (SSL) proteins including Adipocyte plasma membrane- associated proteins (APMAPs) from animals, Protein STRICTOSIDINE SYNTHASE-LIKE (SSLs) from Arabidopsis and SGL proteins, being also present in bacterial sequences. It is about 50 amino acids in length. It contains residues involved in metal coordination in the active site. This domain is also found in Gluconolactonase and Sugar lactone lactonase. These proteins share a six-bladed beta-propeller fold structure and have similar structural and mechanistic features to SS (strictosidine synthase) that involve nucleophilic attack on an electrophilic substrate, although they do not catalyze the SS reaction as they lack the catalytic glutamate required for SS activity; they catalyze hydrolytic reactions instead. APMAPs shows similarity with paraoxonases (PON) and has a strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. They are involved in adipocyte differentiation.


Pssm-ID: 437899 [Multi-domain]  Cd Length: 46  Bit Score: 37.85  E-value: 4.51e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
gi 665395316   53 NNHLNGARKLWKDQIFGPECLIVLED-KIYTGIHSGEVIRL 92
Cdd:pfam20067   6 NDRLAGAELIALGGEHGPEDIAVDPDgRLYTGLHDGRIVRM 46
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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