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Conserved domains on  [gi|1834199414|ref|NP_729938|]
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nuclear export factor 3, isoform D [Drosophila melanogaster]

Protein Classification

nuclear transport factor 2 family protein( domain architecture ID 233)

nuclear transport factor 2 (NTF2) family protein, similar to Aspergillus flavus scytalone dehydratases, Pseudomonas putida benzene 1,2-dioxygenase subunit beta, and many other members with similar structural details but divergent functions

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
NTF2_like super family cl09109
Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 ...
 338-489 5.02e-17

Nuclear transport factor 2 (NTF2-like) superfamily. This family includes members of the NTF2 family, Delta-5-3-ketosteroid isomerases, Scytalone Dehydratases, and the beta subunit of Ring hydroxylating dioxygenases. This family is a classic example of divergent evolution wherein the proteins have many common structural details but diverge greatly in their function. For example, nuclear transport factor 2 (NTF2) mediates the nuclear import of RanGDP and binds to both RanGDP and FxFG repeat-containing nucleoporins while Ketosteroid isomerases catalyze the isomerization of delta-5-3-ketosteroid to delta-4-3-ketosteroid, by intramolecular transfer of the C4-beta proton to the C6-beta position. While the function of the beta sub-unit of the Ring hydroxylating dioxygenases is not known, Scytalone Dehydratases catalyzes two reactions in the biosynthetic pathway that produces fungal melanin. Members of the NTF2-like superfamily are widely distributed among bacteria, archaea and eukaryotes.


The actual alignment was detected with superfamily member cd00780:

Pssm-ID: 471850  Cd Length: 119  Bit Score: 77.32  E-value: 5.02e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414 338 NGLKFCQDFISSYYTFFDdtEERFKLKKYYDDQAMFSLSVPVQlnyVYGYK------LYNRNQKRQHssfaqnaklqvgr 411
Cdd:cd00780     1 SAEDVAKAFVQQYYSIFD--NNREGLHRLYGDTSMLSREGMKQ---VTGRDaiveklSSLPFQKTKH------------- 62

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1834199414 412 aalllalsrlplmhtDLENVglDIQVFTSSLRIFTLTGYFKEissDTLEPRRFQRTFVLQTSNSpGWLITNDMLCITS 489
Cdd:cd00780    63 ---------------KITTV--DSQPTPSGGVIVMVTGSLKL---DEQPPRKFSQTFVLAPQNG-GYFVLNDIFRFVD 119
LRR super family cl34836
Leucine-rich repeat (LRR) protein [Transcription];
229-300 2.27e-03

Leucine-rich repeat (LRR) protein [Transcription];


The actual alignment was detected with superfamily member COG4886:

Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 40.69  E-value: 2.27e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1834199414 229 FPHVTSLVLSNNYLCSLKAFAGNSqsfaSLERLDISANRIQDLGELNYINKLscKTIFLAGNGLAKLSVDVI 300
Cdd:COG4886   227 LTNLETLDLSNNQLTDLPELGNLT----NLEELDLSNNQLTDLPPLANLTNL--KTLDLSNNQLTDLKLKEL 292
 
Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 338-489 5.02e-17

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


Pssm-ID: 238403  Cd Length: 119  Bit Score: 77.32  E-value: 5.02e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414 338 NGLKFCQDFISSYYTFFDdtEERFKLKKYYDDQAMFSLSVPVQlnyVYGYK------LYNRNQKRQHssfaqnaklqvgr 411
Cdd:cd00780     1 SAEDVAKAFVQQYYSIFD--NNREGLHRLYGDTSMLSREGMKQ---VTGRDaiveklSSLPFQKTKH------------- 62

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1834199414 412 aalllalsrlplmhtDLENVglDIQVFTSSLRIFTLTGYFKEissDTLEPRRFQRTFVLQTSNSpGWLITNDMLCITS 489
Cdd:cd00780    63 ---------------KITTV--DSQPTPSGGVIVMVTGSLKL---DEQPPRKFSQTFVLAPQNG-GYFVLNDIFRFVD 119
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
229-300 2.27e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 40.69  E-value: 2.27e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1834199414 229 FPHVTSLVLSNNYLCSLKAFAGNSqsfaSLERLDISANRIQDLGELNYINKLscKTIFLAGNGLAKLSVDVI 300
Cdd:COG4886   227 LTNLETLDLSNNQLTDLPELGNLT----NLEELDLSNNQLTDLPPLANLTNL--KTLDLSNNQLTDLKLKEL 292
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 192-296 5.05e-03

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 39.00  E-value: 5.05e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414 192 RTLDLSRFHASPELSLHFCPlhmvkllETVLVLSNHLfphvTSLVLSNNYLCSLKAFAGnsqsFASLERLDISANRIQDL 271
Cdd:cd21340    93 EELHIENQRLPPGEKLTFDP-------RSLAALSNSL----RVLNISGNNIDSLEPLAP----LRNLEQLDASNNQISDL 157

                          90       100
                  ....*....|....*....|....*..
gi 1834199414 272 GEL-NYINKLSC-KTIFLAGNGLAKLS 296
Cdd:cd21340   158 EELlDLLSSWPSlRELDLTGNPVCKKP 184
 
Name Accession Description Interval E-value
NTF2 cd00780
Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of ...
 338-489 5.02e-17

Nuclear transport factor 2 (NTF2) domain plays an important role in the trafficking of macromolecules, ions and small molecules between the cytoplasm and nucleus. This bi-directional transport of macromolecules across the nuclear envelope requires many soluble factors that includes GDP-binding protein Ran (RanGDP). RanGDP is required for both import and export of proteins and poly(A) RNA. RanGDP also has been implicated in cell cycle control, specifically in mitotic spindle assembly. In interphase cells, RanGDP is predominately nuclear and thought to be GTP bound, but it is also present in the cytoplasm, probably in the GDP-bound state. NTF2 mediates the nuclear import of RanGDP. NTF2 binds to both RanGDP and FxFG repeat-containing nucleoporins.


Pssm-ID: 238403  Cd Length: 119  Bit Score: 77.32  E-value: 5.02e-17
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414 338 NGLKFCQDFISSYYTFFDdtEERFKLKKYYDDQAMFSLSVPVQlnyVYGYK------LYNRNQKRQHssfaqnaklqvgr 411
Cdd:cd00780     1 SAEDVAKAFVQQYYSIFD--NNREGLHRLYGDTSMLSREGMKQ---VTGRDaiveklSSLPFQKTKH------------- 62

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1834199414 412 aalllalsrlplmhtDLENVglDIQVFTSSLRIFTLTGYFKEissDTLEPRRFQRTFVLQTSNSpGWLITNDMLCITS 489
Cdd:cd00780    63 ---------------KITTV--DSQPTPSGGVIVMVTGSLKL---DEQPPRKFSQTFVLAPQNG-GYFVLNDIFRFVD 119
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
229-300 2.27e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 40.69  E-value: 2.27e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1834199414 229 FPHVTSLVLSNNYLCSLKAFAGNSqsfaSLERLDISANRIQDLGELNYINKLscKTIFLAGNGLAKLSVDVI 300
Cdd:COG4886   227 LTNLETLDLSNNQLTDLPELGNLT----NLEELDLSNNQLTDLPPLANLTNL--KTLDLSNNQLTDLKLKEL 292
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
230-296 3.66e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 39.92  E-value: 3.66e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1834199414 230 PHVTSLVLSNNYLCSLKAFAGNsqsFASLERLDISANRIQDLGELNYINKLscKTIFLAGNGLAKLS 296
Cdd:COG4886   205 TNLEELDLSGNQLTDLPEPLAN---LTNLETLDLSNNQLTDLPELGNLTNL--EELDLSNNQLTDLP 266
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
90-309 3.96e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 39.92  E-value: 3.96e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414  90 VSNSSIFVALKWVMMPIKVEIFNFKRSGPEDNMARGQFLLDNLLSANRLKRLQEEVAVINTCQKIEVRVTPGLPKSMMCA 169
Cdd:COG4886     2 LLLLLSLTLKLLLLLLLELLTTLILLLLLLLLLLALLLLSLLSLLLLLTLLLSLLLRDLLLSSLLLLLSLLLLLLLSLLL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414 170 QITDEFTTAIQEALRSRYEVD-SRTLDLSRFHASPELSLHFCPLhmvklleTVLVLSNHLFPHVTSLVLSNNYLCSLKAF 248
Cdd:COG4886    82 LSLLLLGLTDLGDLTNLTELDlSGNEELSNLTNLESLDLSGNQL-------TDLPEELANLTNLKELDLSNNQLTDLPEP 154

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1834199414 249 AGNsqsFASLERLDISANRIQDLG-ELNYINKLscKTIFLAGNGLAKLSvdvirKMLPQLKN 309
Cdd:COG4886   155 LGN---LTNLKSLDLSNNQLTDLPeELGNLTNL--KELDLSNNQITDLP-----EPLGNLTN 206
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 192-296 5.05e-03

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 39.00  E-value: 5.05e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1834199414 192 RTLDLSRFHASPELSLHFCPlhmvkllETVLVLSNHLfphvTSLVLSNNYLCSLKAFAGnsqsFASLERLDISANRIQDL 271
Cdd:cd21340    93 EELHIENQRLPPGEKLTFDP-------RSLAALSNSL----RVLNISGNNIDSLEPLAP----LRNLEQLDASNNQISDL 157

                          90       100
                  ....*....|....*....|....*..
gi 1834199414 272 GEL-NYINKLSC-KTIFLAGNGLAKLS 296
Cdd:cd21340   158 EELlDLLSSWPSlRELDLTGNPVCKKP 184
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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