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Conserved domains on  [gi|24650150|ref|NP_733121|]
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uncharacterized protein Dmel_CG31380 [Drosophila melanogaster]

Protein Classification

protein kinase family protein( domain architecture ID 10503049)

protein kinase family protein, similar to Bombyx mori EcKinase that catalyzes the phosphorylation of the steroid hormone and together with ecdysteroid-phosphate phosphatase, plays an important role in ecdysteroid economy of the ovary-egg system

CATH:  1.10.510.10
EC:  2.7.-.-
Gene Ontology:  GO:0016310|GO:0005524|GO:0016301
PubMed:  16244704
SCOP:  4003661

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 36-314 3.80e-88

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


:

Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 268.75  E-value: 3.80e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    36 KGENYGAILTRIHLVY----SSIVEEHLIAKTVLEyEDAETKMKKaPYDIYNRELEIYEQVLPKLQELAGE-----QLCP 106
Cdd:pfam02958   1 KGDNYASVMLRVTIEYekgdGSKRTKSLIVKTMPD-NEERREMFS-SLNLFTREINMYEKVLPELEALYREagdpfKLAP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   107 KILHIDRQRGA--LIMEDLSYKGFVMAPRLQRLDEQHVSLVLRKLAKMQAASAVLENnllENNFSLTEYDKGFFNR---- 180
Cdd:pfam02958  79 KCYYADLEPEDqvIILEDLSLKGYKNADRLKGLDLEHTKLVLEKLAKFHAASAALKE---LQPEVFKQLKKGLFEEdyvn 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   181 --YTESFSAYFLGCLKSCANYLKTQ-AGYEHHAKLLDELAPYYMGLGLRCFKQEQTHINVLTHGDLWTNNMMFKY-EAGV 256
Cdd:pfam02958 156 gaIKEFFEPLMETGLDAAAEALREQlPEYEKYAEKLEKLKDNYFDRLLRLVEPTPGEFNVLNHGDLWVNNIMFKYdDEGE 235

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 24650150   257 PSDVLLIDFQYAFWGSPTLDIHHLLNTSAVEQVRSELQMKMRGVYHDVFVGELQRLGF 314
Cdd:pfam02958 236 PEDVILVDFQLSRYGSPAIDLNYFLYTSTELELRLEHFDELLRYYHSSLVETLKKLGY 293
 
Name Accession Description Interval E-value
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 36-314 3.80e-88

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 268.75  E-value: 3.80e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    36 KGENYGAILTRIHLVY----SSIVEEHLIAKTVLEyEDAETKMKKaPYDIYNRELEIYEQVLPKLQELAGE-----QLCP 106
Cdd:pfam02958   1 KGDNYASVMLRVTIEYekgdGSKRTKSLIVKTMPD-NEERREMFS-SLNLFTREINMYEKVLPELEALYREagdpfKLAP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   107 KILHIDRQRGA--LIMEDLSYKGFVMAPRLQRLDEQHVSLVLRKLAKMQAASAVLENnllENNFSLTEYDKGFFNR---- 180
Cdd:pfam02958  79 KCYYADLEPEDqvIILEDLSLKGYKNADRLKGLDLEHTKLVLEKLAKFHAASAALKE---LQPEVFKQLKKGLFEEdyvn 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   181 --YTESFSAYFLGCLKSCANYLKTQ-AGYEHHAKLLDELAPYYMGLGLRCFKQEQTHINVLTHGDLWTNNMMFKY-EAGV 256
Cdd:pfam02958 156 gaIKEFFEPLMETGLDAAAEALREQlPEYEKYAEKLEKLKDNYFDRLLRLVEPTPGEFNVLNHGDLWVNNIMFKYdDEGE 235

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 24650150   257 PSDVLLIDFQYAFWGSPTLDIHHLLNTSAVEQVRSELQMKMRGVYHDVFVGELQRLGF 314
Cdd:pfam02958 236 PEDVILVDFQLSRYGSPAIDLNYFLYTSTELELRLEHFDELLRYYHSSLVETLKKLGY 293
CHK smart00587
ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases
 118-311 6.76e-60

ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases


Pssm-ID: 214734  Cd Length: 196  Bit Score: 192.93  E-value: 6.76e-60
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    118 LIMEDLSYKGFVMAPRLQRLDEQHVSLVLRKLAKMQAASAVLENnlLENNFSLTEYDKGFFNRYTESFSAYFLGCLKSCA 197
Cdd:smart00587   1 IIFEDLSPKGYVNADRLKGLDLEHTSLVLKKLAKLHAASAVLIE--EEKGSYLEEFDEGLFERFKRMFSEEFIGGLENFL 78

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    198 NYLKTQAGY---EHHAKLLDELAPYYMGLGLRCFKQEQTHINVLTHGDLWTNNMMFKYEA-GVPSDVLLIDFQYAFWGSP 273
Cdd:smart00587  79 RELLSQPELlkvEEYIEKLDKLLDNLEDLKKEDKEPDEGEFNVLNHGDLWANNIMFKYDDeGKPEDVALIDFQLSHYGSP 158

                          170       180       190
                   ....*....|....*....|....*....|....*...
gi 24650150    274 TLDIHHLLNTSAVEQVRSELQMKMRGVYHDVFVGELQR 311
Cdd:smart00587 159 AEDLHYFLLTSLSVEIRREHFDELLKFYYETLVETLKK 196
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 206-282 2.11e-04

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 41.52  E-value: 2.11e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 24650150 206 YEHHAKLLDELApyymglglRCFKQEQTH-INVLTHGDLWTNNMMFKYEAGVpsdVLLIDFQYAFWGSPTLDIHHLLN 282
Cdd:cd05120  88 EEEKEKIADQLA--------EILAALHRIdSSVLTHGDLHPGNILVKPDGKL---SGIIDWEFAGYGPPAFDYAAALR 154
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
201-286 2.00e-03

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 38.61  E-value: 2.00e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150 201 KTQAGYEHHAKLLDELAPYYMGLGLRCfkqeqthinVLTHGDLWTNNMMFKYEagvpSDVLLIDFQYAFWGSPTLDIHHL 280
Cdd:COG0510  23 LGPRDLPELLRRLEELERALAARPLPL---------VLCHGDLHPGNFLVTDD----GRLYLIDWEYAGLGDPAFDLAAL 89


                ....*.
gi 24650150 281 LNTSAV 286
Cdd:COG0510  90 LVEYGL 95
 
Name Accession Description Interval E-value
EcKL pfam02958
Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme ...
 36-314 3.80e-88

Ecdysteroid kinase-like family; This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterized substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes.


Pssm-ID: 397213 [Multi-domain]  Cd Length: 293  Bit Score: 268.75  E-value: 3.80e-88
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    36 KGENYGAILTRIHLVY----SSIVEEHLIAKTVLEyEDAETKMKKaPYDIYNRELEIYEQVLPKLQELAGE-----QLCP 106
Cdd:pfam02958   1 KGDNYASVMLRVTIEYekgdGSKRTKSLIVKTMPD-NEERREMFS-SLNLFTREINMYEKVLPELEALYREagdpfKLAP 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   107 KILHIDRQRGA--LIMEDLSYKGFVMAPRLQRLDEQHVSLVLRKLAKMQAASAVLENnllENNFSLTEYDKGFFNR---- 180
Cdd:pfam02958  79 KCYYADLEPEDqvIILEDLSLKGYKNADRLKGLDLEHTKLVLEKLAKFHAASAALKE---LQPEVFKQLKKGLFEEdyvn 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   181 --YTESFSAYFLGCLKSCANYLKTQ-AGYEHHAKLLDELAPYYMGLGLRCFKQEQTHINVLTHGDLWTNNMMFKY-EAGV 256
Cdd:pfam02958 156 gaIKEFFEPLMETGLDAAAEALREQlPEYEKYAEKLEKLKDNYFDRLLRLVEPTPGEFNVLNHGDLWVNNIMFKYdDEGE 235

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 24650150   257 PSDVLLIDFQYAFWGSPTLDIHHLLNTSAVEQVRSELQMKMRGVYHDVFVGELQRLGF 314
Cdd:pfam02958 236 PEDVILVDFQLSRYGSPAIDLNYFLYTSTELELRLEHFDELLRYYHSSLVETLKKLGY 293
CHK smart00587
ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases
 118-311 6.76e-60

ZnF_C4 abd HLH domain containing kinases domain; subfamily of choline kinases


Pssm-ID: 214734  Cd Length: 196  Bit Score: 192.93  E-value: 6.76e-60
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    118 LIMEDLSYKGFVMAPRLQRLDEQHVSLVLRKLAKMQAASAVLENnlLENNFSLTEYDKGFFNRYTESFSAYFLGCLKSCA 197
Cdd:smart00587   1 IIFEDLSPKGYVNADRLKGLDLEHTSLVLKKLAKLHAASAVLIE--EEKGSYLEEFDEGLFERFKRMFSEEFIGGLENFL 78

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150    198 NYLKTQAGY---EHHAKLLDELAPYYMGLGLRCFKQEQTHINVLTHGDLWTNNMMFKYEA-GVPSDVLLIDFQYAFWGSP 273
Cdd:smart00587  79 RELLSQPELlkvEEYIEKLDKLLDNLEDLKKEDKEPDEGEFNVLNHGDLWANNIMFKYDDeGKPEDVALIDFQLSHYGSP 158

                          170       180       190
                   ....*....|....*....|....*....|....*...
gi 24650150    274 TLDIHHLLNTSAVEQVRSELQMKMRGVYHDVFVGELQR 311
Cdd:smart00587 159 AEDLHYFLLTSLSVEIRREHFDELLKFYYETLVETLKK 196
DUF1679 pfam07914
Uncharacterized oxidoreductase dhs-27; The region featured in this family is found in a number ...
 145-310 2.46e-07

Uncharacterized oxidoreductase dhs-27; The region featured in this family is found in a number of C. elegans proteins, in one case as a repeat. In many of the family members, this region is associated with the CHK region described by SMART as being found in ZnF_C4 and HLH domain-containing kinases. In fact, one member of this family is annotated as being a member of the nuclear hormone receptor family, and contains regions typical of such proteins (Interpro:IPR000536, Interpro:IPR008946, and Interpro:IPR001628).


Pssm-ID: 369592  Cd Length: 413  Bit Score: 52.28  E-value: 2.46e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   145 VLRKLAKMQAASavlennllennFSLTEYDKGFFN--RYTESFSAYFLG--CLKSCANYLKTQAGYEHHAK---LLDELA 217
Cdd:pfam07914 177 VLRAIATFQALG-----------ESLSEEEKKSANgaDFLEEMFETFMSeeGLKGIFEQLRNIFGAAYPEKveeLVDIFE 245

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150   218 PYYMGLGLrcFKqEQTHIN-------VLTHGDLWTNNMMFKYEA-GVPSDVLLIDFQYAFWGSPTLDIHHLLNTSAVEQV 289
Cdd:pfam07914 246 HYGPEILI--FK-KYTNLNkvlgikpVLVHGDLWQSNILWTLENdGKLKLKAIIDYQTVHMGNPAEDLVRLLLSCLSGAD 322

                         170       180
                  ....*....|....*....|.
gi 24650150   290 RSELQMKMRGVYHDVFVGELQ 310
Cdd:pfam07914 323 RRAHWEELLEQYYETFTKALG 343
APH pfam01636
Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance ...
 237-299 7.05e-05

Phosphotransferase enzyme family; This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation. This family also includes homoserine kinase. This family is related to fructosamine kinase pfam03881.


Pssm-ID: 426359 [Multi-domain]  Cd Length: 239  Bit Score: 44.03  E-value: 7.05e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 24650150   237 VLTHGDLWTNNMMFKYEAGVpsdVLLIDFQYAFWGSPTLDIHHLLNTSAVEQVRSELQMKMRG 299
Cdd:pfam01636 168 VLVHGDLHPGNLLVDPGGRV---SGVIDFEDAGLGDPAYDLAILLNSWGRELGAELLAAYLAA 227
APH_ChoK_like cd05120
Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ...
 206-282 2.11e-04

Aminoglycoside 3'-phosphotransferase and Choline Kinase family; This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).


Pssm-ID: 270690 [Multi-domain]  Cd Length: 158  Bit Score: 41.52  E-value: 2.11e-04
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 24650150 206 YEHHAKLLDELApyymglglRCFKQEQTH-INVLTHGDLWTNNMMFKYEAGVpsdVLLIDFQYAFWGSPTLDIHHLLN 282
Cdd:cd05120  88 EEEKEKIADQLA--------EILAALHRIdSSVLTHGDLHPGNILVKPDGKL---SGIIDWEFAGYGPPAFDYAAALR 154
CotS COG0510
Thiamine kinase or a related kinase [Coenzyme transport and metabolism];
201-286 2.00e-03

Thiamine kinase or a related kinase [Coenzyme transport and metabolism];


Pssm-ID: 440276 [Multi-domain]  Cd Length: 156  Bit Score: 38.61  E-value: 2.00e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 24650150 201 KTQAGYEHHAKLLDELAPYYMGLGLRCfkqeqthinVLTHGDLWTNNMMFKYEagvpSDVLLIDFQYAFWGSPTLDIHHL 280
Cdd:COG0510  23 LGPRDLPELLRRLEELERALAARPLPL---------VLCHGDLHPGNFLVTDD----GRLYLIDWEYAGLGDPAFDLAAL 89


                ....*.
gi 24650150 281 LNTSAV 286
Cdd:COG0510  90 LVEYGL 95
YcbJ COG3173
Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction ...
 237-281 6.20e-03

Predicted kinase, aminoglycoside phosphotransferase (APT) family [General function prediction only];


Pssm-ID: 442406 [Multi-domain]  Cd Length: 284  Bit Score: 38.17  E-value: 6.20e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 24650150 237 VLTHGDLWTNNMMFKYEAGVPSDVllIDFQYAFWGSPTLDIHHLL 281
Cdd:COG3173 193 VLVHGDLRPGNLLVDPDDGRLTAV--IDWELATLGDPAADLAYLL 235
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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